SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_074200794.1 NCBI__GCF_900141795.1:WP_074200794.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
66% identity, 98% coverage: 4:308/310 of query aligns to 1:306/306 of 4whxA

query
sites
4whxA

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active site: F37 (= F40), K160 (= K162), E194 (= E196), L217 (= L219)
binding alanine: Y97 (= Y99), K160 (= K162), A258 (= A260)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
53% identity, 97% coverage: 8:308/310 of query aligns to 3:304/305 of 2ej0B

query
sites
2ej0B

R

K

D

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active site: F35 (= F40), G37 (= G42), K158 (= K162), E192 (= E196), L215 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R63), Y163 (= Y167), E192 (= E196), G195 (= G199), E196 (= E200), L215 (= L219), G217 (= G221), I218 (= I222), T219 (= T223), G254 (= G258), T255 (= T259)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
51% identity, 97% coverage: 8:308/310 of query aligns to 3:296/297 of 2ej3A

query
sites
2ej3A

R

K

D

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active site: F35 (= F40), G37 (= G42), K150 (= K162), E184 (= E196), L207 (= L219)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G199), G246 (= G258), T247 (= T259), A248 (= A260)
binding pyridoxal-5'-phosphate: R58 (= R63), K150 (= K162), Y155 (= Y167), E184 (= E196), G187 (= G199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G258), T247 (= T259)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
51% identity, 97% coverage: 8:308/310 of query aligns to 3:296/297 of 2eiyA

query
sites
2eiyA

R

K

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A

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A

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A

A

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A

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A

A

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R
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active site: F35 (= F40), G37 (= G42), K150 (= K162), E184 (= E196), L207 (= L219)
binding 4-methyl valeric acid: F35 (= F40), Y94 (= Y99), T247 (= T259), A248 (= A260)
binding pyridoxal-5'-phosphate: R58 (= R63), K150 (= K162), Y155 (= Y167), E184 (= E196), G187 (= G199), E188 (= E200), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G258), T247 (= T259)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
51% identity, 97% coverage: 8:308/310 of query aligns to 3:296/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

L

L

I

I

W

W

F

M

D

N

G

G

E

A

M

F

V

V

P

P

W

Q

R

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

M

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

E

E

S

T

E

A

L

K

G

G

T

P

A

A

I

I

F

F

R

R

L

L

E

K

E

E

H

H

T

V

Q

K

R
|
R

L

F

I

Y

N

N

S

S

A

A

K

K

I

V

M

L

Q

R

M

M

P

E

L

I

N

P

Y

F

T

A

A

P

E

E

L

L

N

E

E

E

A

A

Q

I

R

K

A

E

A

V

V

V

R

R

E

R

N

N

G

G

L

Y

R

R

S

S

A

C

Y

Y

I

I

R

R

P

P

M

L

V

A

F

W

Y

M

G

G

S

A

E

K

G

A

M

L

G

G

L

V

R

N

A

P

-

L

D

P

N

N

L

N

K

P

A

A

H

E

V

V

I

M

V

V

A

A

W

W

E

E

W

W

G

-

A

-

Y

-

M

-

G

-

E

-

N

-

L

V

K

R

R

K

G

G

I

A

K

R

V

L

A

I

T

T

S

S

F

W

T

A

R

R

H

F

H

P

V

A

N

N

I

V

T

M

M

P

T

G

R

K

A

A

K
|
K

A

V

N

G

G

G

N

N

Y
|
Y

I

V

N

N

S

S

M

A

L

L

A

A

L

K

Q

M

E

E

A

A

I

V

S

A

H

A

G

G

A

A

D

D

E

E

A

A

L

L

D

D

P

E

E

E

G

G

Y

Y

V

V

A

A

E
|
E

G

G

S

S

G
|
G

E

E

N

N

F

L

F

F

M

F

I

V

K

R

D

D

G

G

V

V

I

I

Y

Y

T

A

P

L

E

E

L

H

T

S

S

V

C

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

K

D

T

S

V

V

M

I

Q

R

L

I

A

A

R

K

E

D

M

L

G

G

Y

Y

E

E

I

V

V

Q

E

V

K

V

R

R

I

A

T

T

R

R

D

D

E

Q

V

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

T

T

G

G

T
|
T

A

A

E

E

V

V

T

T

P

P

I

V

R

S

E

M

L

I

D

D

G

W

R

R

V

P

I

I

G

G

A

K

G

G

S

T

R

A

G

G

P

P

I

V

T

A

E

L

K

R

L

L

Q

R

Q

E

R

V

Y

Y

F

L

D

E

V

A

V

V

H

T

G

G

R

R

A

R

P

P

E

E

H

Y

R

E

R

G

W

W

L

L

T

T

P

Y

V

V

active site: F35 (= F40), G37 (= G42), K150 (= K162), E184 (= E196), L207 (= L219)
binding pyridoxal-5'-phosphate: R58 (= R63), K150 (= K162), Y155 (= Y167), E184 (= E196), G187 (= G199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), T247 (= T259)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
52% identity, 97% coverage: 8:308/310 of query aligns to 3:293/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

L

L

I

I

W

W

F

M

D

N

G

G

E

A

M

F

V

V

P

P

W

Q

R

E

E

E

A

A

K

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

M

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

E

E

S

T

E

A

L

K

G

G

T

P

A

A

I

I

F

F

R

R

L

L

E

K

E

E

H

H

T

V

Q

K

R
|
R

L

F

I

Y

N

N

S

S

A

A

K

K

I

V

M

L

Q

R

M

M

P

E

L

I

N

P

Y

F

T

A

A

P

E

E

L

L

N

E

E

E

A

A

Q

I

R

K

A

E

A

V

V

V

R

R

E

R

N

N

G

G

L

Y

R

R

S

S

A

C

Y
|
Y

I

I

R

R

P

P

M

L

V

A

F

W

Y

M

G

G

S

A

E

K

G

A

M

L

G

G

L

V

R

N

A

P

-

L

D

P

N

N

L

N

K

P

A

A

H

E

V

V

I

M

V

V

A

A

W

W

E

E

W

W

G

G

A

A

Y

-

M

-

G

-

E

-

N

-

L

-

K

-

R

-

G

-

I

-

K

R

V

L

A

I

T

T

S

S

F

W

T

A

R

R

H

F

H

P

V

A

N

N

I

V

T

M

M

P

T

G

R

K

A

A

K
|
K

A

V

N

G

G

G

N

N

Y
|
Y

I

V

N

N

S

S

M

A

L

L

A

A

L

K

Q

M

E

E

A

A

I

V

S

A

H

A

G

G

A

A

D

D

E

E

A

A

L

L

D

D

P

E

E

E

G

G

Y

Y

V

V

A

A

E
|
E

G

G

S

S

G
|
G

E
|
E

N

N

F

L

F

F

M

F

I

V

K

R

D

D

G

G

V

V

I

I

Y

Y

T

A

P

L

E

E

L

H

T

S

S

V

C

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

K

D

T

S

V

V

M

I

Q

R

L

I

A

A

R

K

E

D

M

L

G

G

Y

Y

E

E

I

V

V

Q

E

V

K

V

R

R

I

A

T

T

R

R

D

D

E

Q

V

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

I

V

R

S

E

M

L

I

D

D

G

W

R

R

V

P

I

I

G

G

A

K

G

G

S

T

R

A

G

G

P

P

I

V

T

A

E

L

K

R

L

L

Q

R

Q

E

R

V

Y

Y

F

L

D

E

V

A

V

V

H

T

G

G

R

R

A

R

P

P

E

E

H

Y

R

E

R

G

W

W

L

L

T

T

P

Y

V

V

active site: F35 (= F40), G37 (= G42), K147 (= K162), E181 (= E196), L204 (= L219)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R63), Y94 (= Y99), Y152 (= Y167), E181 (= E196), G184 (= G199), E185 (= E200), L204 (= L219), G206 (= G221), I207 (= I222), T208 (= T223), T244 (= T259), A245 (= A260)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
48% identity, 96% coverage: 12:308/310 of query aligns to 5:303/304 of 1iyeA

query
sites
1iyeA

I

I

W

W

F

F

D

N

G

G

E

E

M

M

V

V

P

R

W

W

R

E

E

D

A

A

K

K

V

V

H

H

V

V

L

M

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

M

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

C

Y

Y

E

D

S

S

E

H

L

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

E

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

I

H

N

D

S

S

A

A

K

K

I

I

M

Y

Q

R

M

F

P

P

L

V

N

S

Y

Q

T

S

A

I

E

D

E

E

L

L

N

M

E

E

A

A

Q

C

R

R

A

D

A

V

V

I

R

R

E

K

N

N

G

N

L

L

R

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

M

L

V

I

F

F

Y

V

G

G

S

D

E

V

G

G

M

M

G

G

L

V

R

N

A

P

D

P

-

A

N

G

L

Y

K

S

A

T

H

D

V

V

I

I

V

I

A

A

W

F

E

P

W

W

G

G

A

A

Y
|
Y

M

L

G

G

E

A

E

E

N

A

L

L

K

E

R

Q

G

G

I

I

K

D

V

A

A

M

T

V

S

S

F

W

T

N

R

R

H

A

V

P

N

N

I

T

T

I

M

P

T

T

R

A

A

A

K
|
K

A
|
A

N

G

G

G

N

N

Y
|
Y

I

L

N

S

S

S

M

L

L

L

A

V

L

G

Q

S

E

E

A

A

I

R

S

R

H

H

G

G

A

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

P

V

E

N

G

G

Y

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E
|
E

N
|
N

F

L

F

F

M

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

T

T

P

P

E

P

L

F

T

T

-

S

S

S

C

A

L
|
L

D

P

G
|
G

I
|
I

T
|
T

R

R

K

D

T

A

V

I

M

I

Q

K

L

L

A

A

R

K

E

E

M

L

G

G

Y

I

E

E

I

V

V

R

E

E

K

Q

R

V

I

L

T

S

R

R

D

E

E

S

V

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

G

G

R

I

V

Q

I

V

G

G

A

E

G

G

S

R

R

C

G

G

P

P

I

V

T

T

E

K

K

R

L

I

Q

Q

R

A

Y

F

F

F

D

G

V

L

V

F

H

T

G

G

R

E

A

T

P

E

E

D

H

K

R

W

R

G

W

W

L

L

T

D

P

Q

V

V

active site: F33 (= F40), G35 (= G42), K156 (= K162), A157 (= A163), E190 (= E196), L214 (= L219)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R63), Y92 (= Y99), Y126 (= Y132), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), E194 (= E200), N195 (= N201), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (= T259), A255 (= A260)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
48% identity, 96% coverage: 12:308/310 of query aligns to 5:303/304 of 1iydA

query
sites
1iydA

I

I

W

W

F

F

D

N

G

G

E

E

M

M

V

V

P

R

W

W

R

E

E

D

A

A

K

K

V

V

H

H

V

V

L

M

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

M

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

C

Y

Y

E

D

S

S

E

H

L

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

E

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

I

H

N

D

S

S

A

A

K

K

I

I

M

Y

Q

R

M

F

P

P

L

V

N

S

Y

Q

T

S

A

I

E

D

E

E

L

L

N

M

E

E

A

A

Q

C

R

R

A

D

A

V

V

I

R

R

E

K

N

N

G

N

L

L

R

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

M

L

V

I

F

F

Y

V

G

G

S

D

E

V

G

G

M

M

G

G

L

V

R

N

A

P

D

P

-

A

N

G

L

Y

K

S

A

T

H

D

V

V

I

I

V

I

A

A

W

F

E

P

W

W

G

G

A

A

Y
|
Y

M

L

G

G

E

A

E

E

N

A

L

L

K

E

R

Q

G

G

I

I

K

D

V

A

A

M

T

V

S

S

F

W

T

N

R

R

H

A

V

P

N

N

I

T

T

I

M

P

T

T

R

A

A

A

K
|
K

A
|
A

N

G

G

G

N

N

Y
|
Y

I

L

N

S

S

S

M

L

L

L

A

V

L

G

Q

S

E

E

A

A

I

R

S

R

H

H

G

G

A

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

P

V

E

N

G

G

Y

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E
|
E

N

N

F

L

F

F

M

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

T

T

P

P

E

P

L

F

T

T

-

S

S

S

C

A

L
|
L

D

P

G
|
G

I
|
I

T
|
T

R

R

K

D

T

A

V

I

M

I

Q

K

L

L

A

A

R

K

E

E

M

L

G

G

Y

I

E

E

I

V

V

R

E

E

K

Q

R

V

I

L

T

S

R

R

D

E

E

S

V

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

G

G

R

I

V

Q

I

V

G

G

A

E

G

G

S

R

R

C

G

G

P

P

I

V

T

T

E

K

K

R

L

I

Q

Q

R

A

Y

F

F

F

D

G

V

L

V

F

H

T

G

G

R

E

A

T

P

E

E

D

H

K

R

W

R

G

W

W

L

L

T

D

P

Q

V

V

active site: F33 (= F40), G35 (= G42), K156 (= K162), A157 (= A163), E190 (= E196), L214 (= L219)
binding glutaric acid: Y92 (= Y99), Y126 (= Y132), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R63), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), E194 (= E200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T259)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
48% identity, 96% coverage: 12:308/310 of query aligns to 5:303/304 of 1i1mA

query
sites
1i1mA

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active site: K156 (= K162)
binding 4-methyl valeric acid: Y92 (= Y99), K156 (= K162), T254 (= T259), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R63), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), E194 (= E200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (= T259)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
48% identity, 96% coverage: 12:308/310 of query aligns to 5:303/304 of 1i1lA

query
sites
1i1lA

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I

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active site: K156 (= K162)
binding 2-methylleucine: Y92 (= Y99), K156 (= K162), T254 (= T259), A255 (= A260)
binding pyridoxal-5'-phosphate: R56 (= R63), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T259)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
46% identity, 96% coverage: 12:308/310 of query aligns to 9:300/301 of 6thqB

query
sites
6thqB

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active site: F37 (= F40), K156 (= K162), E190 (= E196), L214 (= L219)
binding pyridoxal-5'-phosphate: R60 (= R63), K156 (= K162), Y161 (= Y167), E190 (= E196), N195 (= N201), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T259)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R63), Y97 (= Y99), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), E194 (= E200), N195 (= N201), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G258), T254 (= T259), A255 (= A260)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
41% identity, 90% coverage: 12:290/310 of query aligns to 4:277/290 of 5mr0D

query
sites
5mr0D

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E

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W

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A

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K

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M

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I

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A

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A

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K

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A

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D

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A

A

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L

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G

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Y

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x
D

N

N

F

I

F

F

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I

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K

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N

G

G

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A

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L

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T

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C

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R

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N

I

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D

Y

E

D

V

L

Y

Y

I

T

A

A

D

D

E

E

A

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F

F

F

V

T

T

G
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A

A

E

E

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A

P

P

I

I

R

V

E

V

L

I

D

D

G

G

R

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K

I

I

G

G

A

D

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G

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K

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F

active site: F32 (= F40), G34 (= G42), K150 (= K162), E183 (= E196), L206 (= L219)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R63), G100 (= G112), L101 (= L113), K150 (= K162), Y154 (= Y167), E183 (= E196), G186 (= G199), D187 (≠ E200), L206 (= L219), I209 (= I222), T210 (= T223), G245 (= G258), T246 (= T259)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
39% identity, 90% coverage: 11:290/310 of query aligns to 4:278/290 of 5e25A

query
sites
5e25A

L

L

I

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Y

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N

G

G

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E

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A

A

K

K

V

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H

H

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G

L

F

H

L

Y

Y

G

G

M

D

G

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

E

N

S

G

E

K

L

-

G

-

T

-

A

-

I

V

F

F

R

K

L

L

E

Y

E

E

H

H

T

I

Q

D

R
|
R

L

L

I

Y

N

D

S

C

A

A

K

R

I

V

M

I

Q

D

M

L

P

K

L

I

N

P

Y

L

T

S

A

K

E

E

L

F

N

A

E

E

A

A

Q

I

R

L

A

E

A

T

V

L

R

R

E

R

N

N

G

N

L

L

R

R

S

D

A

A

Y
|
Y

I

I

R

R

P

P

M

I

V

V

F

T

Y

R

G

G

S

A

E

G

G

D

M

L

G

G

L

L

R

D

A

P

D

R

N

K

L

C

K

P

A

S

-

P

H

N

V

V

I

I

V

I

A

I

A

T

W

K

E

P

W

W

G

G

A

K

Y

L

M

Y

G

G

E

D

E

L

N

-

L

Y

K

E

R

K

G

G

I

L

K

K

V

A

A

I

T

T

S

V

S

A

F

I

T

R

R

R

H

N

H

A

V

I

N

D

I

S

T

L

M

P

T

P

R

N

A

I

K
|
K

A

S

N

-

G

L

N

N

Y
|
Y

I

L

N

N

S

N

M

I

L

L

A

A

L

K

Q

I

E

E

A

A

I

N

S

A

H

K

G

G

A

G

D

D

E

E

A

A

L

I

L

F

L

L

D

D

P

H

E

N

G

G

Y

Y

V

I

A

S

E
|
E

G

G

S

S

G
|
G

E
x
D

N

N

F

I

F

F

M

I

I

V

K

K

D

N

G

G

V

T

I

I

Y

T

T

T

P

P

E

P

L

T

T

L

S

N

C

N

L
|
L

D

K

G
|
G

I
|
I

T
|
T

R

R

K

Q

T

V

V

V

M

I

Q

E

L

L

A

I

R

N

E

E

M

L

G

E

Y

I

E

P

I

F

V

R

E

E

K

A

R

N

I

I

T

G

R

L

D

F

E

D

V

L

Y

Y

I

S

A

A

D

D

E

E

A

I

F

F

F

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

I

V

R

T

E

Y

L

I

D

D

G

G

R

R

V

T

I

V

G

G

A

N

G

G

S

K

R

P

G

G

P

K

I

V

T

T

E

K

K

M

L

L

Q

M

Q

E

R

K

Y

F

active site: F33 (= F40), G35 (= G42), K151 (= K162), E184 (= E196), L207 (= L219)
binding 2-oxoglutaric acid: Y88 (= Y99), K151 (= K162), T247 (= T259), A248 (= A260)
binding pyridoxal-5'-phosphate: R52 (= R63), K151 (= K162), Y155 (= Y167), E184 (= E196), G187 (= G199), D188 (≠ E200), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G258), T247 (= T259)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
40% identity, 96% coverage: 12:308/310 of query aligns to 6:304/307 of 6q8eA

query
sites
6q8eA

I

L

W

W

F

W

D

N

G

H

E

R

M

I

V

I

P

P

W

W

R

E

E

E

A

A

K

T

V

V

H

H

V

L

L

T

T

D

H

Y

T

W

L

W

H

A

Y

S

G

V

M

T

G

A

V

V

F
|
F

E

E

G

G

V

I

R

R

A

G

Y

Y

-

W

-

N

E

N

S

A

E

E

L

G

G

E

T

M

A

Y

I

I

F

F

R

R

L

L

E

E

E

D

H

H

T

A

Q

R

R
|
R

L

L

I

E

N

Q

S

S

A

M

K

Q

I

L

M

I

Q

R

M

M

P

P

L

K

N

E

Y

F

T

T

A

V

E

D

E

E

L

I

N

C

E

Q

A

A

Q

T

R

I

A

D

A

L

V

V

R

R

E

A

N

N

G

D

L

Y

R

R

-

G

S

D

A

V

Y

Y

I

I

R

M

P

P

M

L

V

A

F

Y

-

A

Y

V

G

G

S

N

E

K

G

A

M

F

G

S

L

V

R

V

A

G

D

D

N

R

L

T

K

T

A

E

H

M

V

F

I

I

V

Y

A

S

A

-

W

-

E

R

W

P

G

A

A

V

Y

S

M

R

G

L

E

E

N

D

L

F

K

-

R

-

G

S

I

L

K

H

V

A

A

C

T

Y

S

S

F

W

T

T

R

R

H

I

H

N

V

E

N

R

I

V

T

L

M

P

T

P

R

R

A

I

K
|
K

A

A

N

L

G

A

N

N

Y
|
Y

I

R

N

N

S

S

M

Q

L

L

A

A

L

S

Q

S

E

E

A

A

I

A

S

M

H

N

G

G

A

Y

D

D

E

T

A

A

L

L

L

F

L

L

D

N

P

P

E

E

G

G

Y

K

V

V

A

A

E
|
E

G

G

S

T

G
|
G

E
x
S

N
x
C

F

V

F

F

M

F

I

V

K

R

D

K

G

G

V

K

I

L

Y

I

T

T

P

P

E

D

L

I

T

T

S

S

-

G

C

I

L
|
L

D

E

G
x
S

I
|
I

T
|
T

R

R

K

D

T

T

V

V

M

I

Q

H

L

L

A

A

R

R

E

E

-

V

M

L

G

G

Y

L

E

E

I

V

V

E

E

E

K

R

R

V

I

V

T

D

R

R

D

T

E

E

V

T

Y

Y

I

L

A

A

D

D

E

E

A

A

F

F

F

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

I

I

R

A

E

S

L

I

D

D

G

R

R

H

V

E

I

M

G

K

A

H

G

G

S

A

R

P

G

G

P

P

I

I

T

T

E

R

K

Q

L

L

Q

R

Q

D

R

I

Y

Y

F

R

D

E

V

V

H

Y

G

G

R

R

A

D

P

F

E

R

H

Y

R

R

R

N

W

W

L

L

T

T

P

P

V

V

active site: F34 (= F40), K156 (= K162), E190 (= E196), L214 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R63), K156 (= K162), Y161 (= Y167), E190 (= E196), G193 (= G199), S194 (≠ E200), C195 (≠ N201), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), G254 (= G258), T255 (= T259)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
39% identity, 96% coverage: 12:308/310 of query aligns to 6:304/309 of 7neaA

query
sites
7neaA

I

L

W

W

F

W

D

N

G

H

E

R

M

I

V

I

P

P

W

W

R

E

E

E

A

A

K

T

V

V

H

H

V

L

L

T

T

D

H

Y

T

W

L

W

H

A

Y

S

G

V

M

T

G

A

V

V

F
|
F

E

E

G

V

V

I

R

S

A

G

Y

Y

-

W

-

N

E

N

S

A

E

E

L

G

G

E

T

M

A

Y

I

I

F

F

R

R

L

L

E

E

E

D

H

H

T

A

Q

R

R
|
R

L

L

I

E

N

Q

S

S

A

M

K

Q

I

L

M

I

Q

R

M

M

P

P

L

K

N

E

Y

F

T

T

A

V

E

D

E

E

L

I

N

C

E

Q

A

A

Q

T

R

I

A

D

A

L

V

V

R

R

E

A

N

N

G

D

L

Y

R

R

-

G

S

D

A

V