SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_074201095.1 NCBI__GCF_900141795.1:WP_074201095.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
78% identity, 99% coverage: 3:275/275 of query aligns to 2:273/274 of 3tdtA

query
sites
3tdtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding 2-amino-6-oxopimelic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)
binding coenzyme a: G185 (= G187), A186 (= A188), S203 (= S205), M204 (= M206), R217 (= R219), V234 (= V236), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
78% identity, 99% coverage: 3:275/275 of query aligns to 2:273/274 of 2tdtA

query
sites
2tdtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding coenzyme a: S159 (= S161), F183 (= F185), G185 (= G187), S203 (= S205), M204 (= M206), R217 (= R219), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)
binding (2S)-2-aminoheptanedioic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
78% identity, 99% coverage: 3:275/275 of query aligns to 2:273/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding pimelic acid: R104 (= R106), M124 (= M126), M139 (= M141)
binding succinyl-coenzyme a: D141 (= D143), S159 (= S161), A186 (= A188), S203 (= S205), R217 (= R219), K259 (= K261), T260 (= T262), K263 (= K265)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
78% identity, 99% coverage: 3:275/275 of query aligns to 2:273/274 of 1kgqA

query
sites
1kgqA

Q

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D

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active site: D141 (= D143), G166 (= G168), E189 (= E191)
binding (2S)-2-aminoheptanedioic acid: R104 (= R106), M124 (= M126), M139 (= M141), D141 (= D143)
binding succinamide-coa: F183 (= F185), G185 (= G187), S203 (= S205), M204 (= M206), R217 (= R219), V234 (= V236), K254 (= K256), K259 (= K261), T260 (= T262), K263 (= K265)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
40% identity, 36% coverage: 99:196/275 of query aligns to 70:168/203 of 3r8yA

query
sites
3r8yA

D

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P

P

L

P

Q

S

A

A

A

K

P

P

T

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

I

V

V

L

E

E

G

G

V

V

active site: D115 (= D143), G140 (= G168), V163 (≠ E191)
binding calcium ion: G121 (= G149), G122 (≠ S150)

Query Sequence

>WP_074201095.1 NCBI__GCF_900141795.1:WP_074201095.1
MYQDLQNTIEQAFENRAEYTPTNAPAQVREAVAEAINLLDSGKLRVAEREGVGQWKVNEW
LKKAVLLSFRLEDNHVIEAPDTKYFDKVPSKFADWNEDDFRTAGIRVVPPAMARKGSFIA
KGAVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQAAPTII
EDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVSGSL
PSRDGSYSLYCAVIVKKVDAKTLSKVGINELLRGV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory