SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_074201116.1 NCBI__GCF_900141795.1:WP_074201116.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
68% identity, 98% coverage: 1:425/432 of query aligns to 1:420/420 of 5i92F

query
sites
5i92F

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active site: V19 (= V19), Y142 (= Y142), E201 (= E204), D234 (= D237), M237 (= M240), K262 (= K265), A395 (= A400)
binding leucine: A417 (≠ C422), F418 (= F423), A420 (= A425)

P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
66% identity, 98% coverage: 1:423/432 of query aligns to 1:423/426 of P23893

query
sites
P23893

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K265 (= K265) mutation to R: 2% of wild-type activity.

6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
64% identity, 96% coverage: 2:416/432 of query aligns to 9:419/430 of 6w80A

query
sites
6w80A

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L

active site: V26 (= V19), Y149 (= Y142), D241 (= D237), K269 (= K265)
binding pyridoxal-5'-phosphate: S121 (= S114), G122 (= G115), T123 (= T116), Y149 (= Y142), H150 (= H143), E208 (= E204), N213 (= N209), D241 (= D237), V243 (= V239), K269 (= K265)

2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 2gsaB

query
sites
2gsaB

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), E206 (= E204), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding pyridoxal-5'-phosphate: G117 (= G115), T118 (= T116), Y144 (= Y142), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265)

2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 2gsaA

query
sites
2gsaA

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), E206 (= E204), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), G146 (= G144), N211 (= N209), D239 (= D237), V241 (= V239), K267 (= K265)

3usfA Crystal structure of dava-4
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 3usfA

query
sites
3usfA

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S
|
S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E

E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S21), V25 (= V23), S157 (= S155), K267 (= K265), E400 (= E399)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G115), T118 (= T116), Y144 (= Y142), N211 (= N209), D239 (= D237), V241 (= V239), K267 (= K265)

3fq7A Gabaculine complex of gsam (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 3fq7A

query
sites
3fq7A

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S21), V25 (= V23), W61 (= W59), G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), G298 (= G296), T299 (= T297), E400 (= E399)

2hp2A Inter-subunit signaling in gsam (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 2hp2A

query
sites
2hp2A

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V

V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: G298 (= G296), T299 (= T297)
binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S21), G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265)
binding pyridoxal-5'-phosphate: G298 (= G296), T299 (= T297)

2hp1A Inter-subunit signaling in gsam (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 2hp1A

query
sites
2hp1A

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R

N

F

Y

E

E

Q

D

V

A

V

K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S21), V25 (= V23), W61 (= W59), S116 (= S114), G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), E400 (= E399)

2hozA Inter-subunit signaling in gsam (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 5:427/427 of 2hozA

query
sites
2hozA

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E
|
E

G

G

G

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G
|
G

S
|
S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T
x
P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

T

G

L

L

R

R

Q

E

V

I

C

T

D

L

A

E

H

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V

V

M
|
M

T

T

G

G

F

F

R

R

V

I

G

A

L

Y

T

G

G

G

A

V

Q

Q

G

E

R

K

Y

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

F

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

M

L

P

P

V

V

G

G

A

A

L

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

S

Q

Q

L

L

V

A

A

P

P

T

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

L

I

T

K

T

T

L

L

K

E

R

L

I

L

S

R

Q

Q

P

P

G

G

F

T

F

Y

E

E

D

Y

L

L

E

D

A

Q

K

I

T

T

Q

K

K

R

L

L

A

S

M

D

G

G

L

L

E

L

Q

A

V

I

A

A

H

Q

E

E

E

T

G

G

I

H

A

A

L

A

T

C

T

G

N

G

Q

Q

V

V

G

S

G

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

A

-

E

E

A

G

P

P

I

V

T

H

R
x
N

F

Y

E
|
E

Q

D

V

A

V
x
K

R

K

S

S

D

D

I

L

E

Q

H

K

F

F

K

S

R

R

F

F

F

H

H

R

M

G

M

M

L

L

D

E

E

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

V

T

S

S

A

L

A

A

H

H

T

T

D

E

E

E

D

D

L

I

E

D

A

A

T

T

L

L

A

A

A

A

A

A

R

R

R

T

C

V

F

M

Q

S

A

A

F

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: E141 (= E139), G156 (= G154), S157 (= S155), P182 (≠ T180), N368 (≠ R367), E370 (= E369), K373 (≠ V372)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), G298 (= G296), T299 (= T297)

3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
59% identity, 98% coverage: 3:426/432 of query aligns to 4:426/426 of 3fqaA

query
sites
3fqaA

R

K

S

S

H

D

Q

E

L

I

F

F

T

A

H

A

A

A

Q

Q

Q

K

H

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V

V

R
|
R

A

A

F

F

R

K

S

S

V

V

E

G

G

G

D

Q

P

P

V

I

F

V

I

F

D

D

R

R

A

V

K

K

G

D

A

A

Y

Y

V

A

W

W

D

D

V

V

D

D

G

G

K

N

R

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

V

I

E

E

A

A

V

L

Q

K

K

V

Q

A

A

M

E

E

K

K

G

G

L

T

S

S

Y

F

G

G

A

A

P

P

T

C

E

A

L

L

E

E

V

N

E

V

M

L

A

A

D

E

L

M

I

V

C

N

E

D

L

A

I

V

P

P

S

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

T

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

A

Y

T

T

G

G

R

R

D

D

R

K

I

I

V

I

K

K

F

F

E

E

G

G

G

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

H

L

G

G

V

L

P

P

S

S

S

S

P

P

G

G

V

V

P

P

K

K

C

K

L

T

A

T

E

A

Q

N

T

T

L

L

T

T

L

T

T

P

Y

Y

N

N

D

D

A

L

E

E

Q

A

V

V

R

K

N

A

V

L

F

F

D

A

E

E

V

N

G

P

D

G

E

E

I

I

A

A

C

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M