SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_075618579.1 NCBI__GCF_001939075.1:WP_075618579.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 86% coverage: 19:253/274 of query aligns to 26:260/265 of P07821

query
sites
P07821

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K50 (= K43) mutation to Q: Lack of activity.
D172 (= D165) mutation to E: Lack of activity.
E173 (= E166) mutation to A: Lack of activity.

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
33% identity, 76% coverage: 24:230/274 of query aligns to 46:252/382 of 7ahhC

query
sites
7ahhC

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binding phosphoaminophosphonic acid-adenylate ester: G62 (= G40), G64 (= G42), K65 (= K43), D187 (= D165), E188 (= E166)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
33% identity, 76% coverage: 24:230/274 of query aligns to 46:252/382 of 7aheC

query
sites
7aheC

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Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
32% identity, 76% coverage: 24:230/274 of query aligns to 46:252/260 of 7ahdC

query
sites
7ahdC

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binding adenosine-5'-triphosphate: S61 (≠ N39), G62 (= G40), G64 (= G42), K65 (= K43), S66 (= S44), T67 (= T45), Q111 (= Q85), K161 (≠ D139), Q162 (≠ T140), S164 (= S142), G166 (= G144), M167 (≠ Q145), Q188 (≠ E166), H221 (= H199)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

5x40A Structure of a cbio dimer bound with amppcp (see paper)
37% identity, 77% coverage: 20:230/274 of query aligns to 21:229/280 of 5x40A

query
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5x40A

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binding phosphomethylphosphonic acid adenylate ester: N40 (= N39), G41 (= G40), G43 (= G42), K44 (= K43), S45 (= S44), T46 (= T45), Q88 (= Q85), H139 (≠ D139), M140 (≠ T140), L141 (= L141), S142 (= S142), G144 (= G144), Q145 (= Q145), Q166 (≠ E166), H198 (= H199)
binding magnesium ion: S45 (= S44), Q88 (= Q85)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14, 18, 20

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
34% identity, 80% coverage: 7:224/274 of query aligns to 8:228/232 of 1f3oA

query
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1f3oA

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K

K

-

I

-

R

-

D

K

K

M

I

G

G

I

F

L

V

P

F

Q

Q

S

Q

P

F

V

N

S

L

P

I

E

P

G

L

L

L

T

T

V

A

H

L

E

E

L

N

V

V

K

E

Q

-

G

-

R

-

Y

L

P

P

H

L

Q

-

T

I

W

F

L

K

K

Y

R

R

W

G

S

A

A

M

E

S

D

G

T

E

E

E

K

R

V

R

E

K

A

R

A

A

M

L

E

E

A

C

T

L

R

K

I

M

S

A

D

E

L

L

R

E

D

E

R

R

A

F

V

A

D

N

-

H

-

K

-

P

-

N

T

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

N

D

N

T

P

D

P

V

I

I

I

L

L

L

A

D

D

E

E

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

T

Q

G

I

E

E

K

I

I

L

M

D

Q

L

L

F

K

E

K

L

L

N

N

E

E

K

E

K

D

E

G

R

K

T

T

I

V

V

V

M

V

V

V

L

T

H

H

D

D

L

I

N

N

L

V

A

A

S

-

R

R

Y

F

A

G

H

E

N

R

I

I

A

Y

I

L

K

K

N

D

G

G

E

E

V

V

H

E

A

R

Q

E

G

E

K

K

binding adenosine-5'-diphosphate: Y11 (≠ L10), G41 (= G40), G43 (= G42), K44 (= K43), S45 (= S44), T46 (= T45)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
30% identity, 83% coverage: 2:228/274 of query aligns to 2:228/263 of 7d0aB

query
sites
7d0aB

Q

Q

T

S

A

L

V

I

R

E

T

V

E

K

S

N

L

L

S

S

I

F

G

N

Y

R

T

G

D

E

H

R

L

V

L

I

F

Y

E

D

N

N

L

I

D

S

L

L

H

N

I

I

P

R

R

R

G

G

E

Q

I

I

S

T

V

A

F

I

V

M

G

G

S

P

N
x
S

G

G

C
x
T

G
|
G

K
|
K

S

T

T

T

L

L

R

R

S

L

I

I

A

G

R

G

L

Q

L

L

K

V

P

P

T

D

T

Q

G

G

S

E

V

V

L

L

E

D

G

G

K

K

D

D

V

I

H

A

H

Q

M

M

S

S

S

R

R

Q

D

E

V

L

A

F

-

A

-

R

K

A

K

R

M

M

G

G

I

M

L

L

P

F

Q

Q

S

S

P

G

V

A

S

L

P

F

E

T

G

D

L

M

T

S

V

V

H

Y

E

E

L

N

V

V

K

A

Q

-

G

-

R

-

Y

F

P

P

H

I

Q

R

T

A

W

H

L

T

K

K

R

L

W

S

S

E

A

N

E

L

D

I

T

A

E

E

K

L

V

V

E

A

A

L

A

K

M

L

E

E

A

S

T

V

R

G

I

L

S

R

D

G

L

T

R

E

D

Q

R

L

A

M

V

P

D

T

T
x
E

L

L

S
|
S

G

G

Q
x
M

R

N

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

T

A

L

I

A

A

Q

L

D

D

T

P

D

D

V

L

I

I

L

M

L

Y

D

D

E

E

P

P

T

F

T

A

Y

G

L

Q

D

D

M

P

T

I

H

V

Q

K

I

G

E

V

I

L

L

T

D

R

L

L

L

I

F

R

E

S

L

L

N

R

E

E

K

A

K

L

E

D

R

L

T

T

I

T

V

I

M

I

V

V

L

S

H

H

D

D

L

V

N

P

L

E

A

T

S

L

R

S

Y

I

A

A

H

D

N

Y

I

I

I

Y

A

V

I

V

K

A

N

E

G

G

E

K

V

I

H

Q

A

G

Q

E

G

G

K

T

P

P

E

E

N

E

I

L

binding adenosine-5'-diphosphate: S39 (≠ N39), T41 (≠ C41), G42 (= G42), K43 (= K43), E140 (≠ T140), S142 (= S142), M145 (≠ Q145)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
30% identity, 83% coverage: 2:228/274 of query aligns to 2:228/263 of 7d08B

query
sites
7d08B

Q

Q

T

S

A

L

V

I

R

E

T

V

E

K

S

N

L

L

S

S

I

F

G

N

Y
x
R

T

G

D

E

H
x
R

L

V

L

I

F

Y

E

D

N

N

L

I

D

S

L

L

H

N

I

I

P

R

R

R

G

G

E

Q

I

I

S

T

V

A

F

I

V

M

G

G

S

P

N

S

G
|
G

C
x
T

G

G

K
|
K

S
x
T

T
|
T

L

L

R

R

S

L

I

I

A

G

R

G

L

Q

L

L

K

V

P

P

T

D

T

Q

G

G

S

E

V

V

L

L

E

D

G

G

K

K

D

D

V

I

H

A

H

Q

M

M

S

S

S

R

R

Q

D

E

V

L

A

F

-

A

-

R

K

A

K

R

M

M

G

G

I

M

L

L

P

F

Q

Q

S

S

P

G

V

A

S

L

P

F

E

T

G

D

L

M

T

S

V

V

H

Y

E

E

L

N

V

V

K

A

Q

-

G

-

R

-

Y

F

P

P

H

I

Q

R

T

A

W

H

L

T

K

K

R

L

W

S

S

E

A

N

E

L

D

I

T

A

E

E

K

L

V

V

E

A

A

L

A

K

M

L

E

E

A

S

T

V

R

G

I

L

S

R

D

G

L

T

R

E

D

Q

R

L

A

M

V

P

D

T

T

E

L

L

S

S

G

G

Q

M

R

N

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

T

A

L

I

A

A

Q

L

D

D

T

P

D

D

V

L

I

I

L

M

L

Y

D

D

E
|
E

P

P

T

F

T

A

Y

G

L

Q

D

D

M

P

T

I

H

V

Q

K

I

G

E

V

I

L

L

T

D

R

L

L

L

I

F

R

E

S

L

L

N

R

E

E

K

A

K

L

E

D

R

L

T

T

I

T

V

I

M

I

V

V

L

S

H

H

D

D

L

V

N

P

L

E

A

T

S

L

R

S

Y

I

A

A

H

D

N

Y

I

I

I

Y

A

V

I

V

K

A

N

E

G

G

E

K

V

I

H

Q

A

G

Q

E

G

G

K

T

P

P

E

E

N

E

I

L

binding adenosine-5'-triphosphate: R14 (≠ Y14), R17 (≠ H17), G40 (= G40), T41 (≠ C41), K43 (= K43), T44 (≠ S44), T45 (= T45), E166 (= E166)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 80% coverage: 7:224/274 of query aligns to 8:228/230 of 1l2tA

query
sites
1l2tA

T

T

E

K

S

T

L
x
Y

S

K

I

M

G

G

Y

E

T

E

D

I

H

I

L

Y

L

A

F

L

E

K

N

N

L

V

D

N

L

L

H

N

I

I

P

K

R

E

G

G

E

E

I

F

S

V

V

S

F

I

V

M

G

G

S

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

S

I

I

I

A

G

R

C

L

L

L

D

K

K

P

P

T

T

T

E

G

G

S

E

V

V

L

Y

L

I

E

D

G

N

K

I

D

K

V

T

H

N

H

D

M

L

S

D

R

D

D

E

V

L

A

T

K

K

-

I

-

R

-

D

K

K

M

I

G

G

I

F

L

V

P

F

Q

Q

S

Q

P

F

V

N

S

L

P

I

E

P

G

L

L

L

T

T

V

A

H

L

E

E

L

N

V

V

K

E

Q

-

G

-

R

-

Y

L

P

P

H

L

Q

-

T

I

W

F

L

K

K

Y

R

R

W

G

S

A

A

M

E

S

D

G

T

E

E

E

K

R

V

R

E

K

A

R

A

A

M

L

E

E

A

C

T

L

R

K

I

M

S

A

D

E

L

L

R

E

D

E

R

R

A
x
F

V

A

D

N

-

H

-

K

-

P

-

N

T
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

N

D

N

T

P

D

P

V

I

I

I

L

L

L

A

D

D

E

Q

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

T

Q

G

I

E

E

K

I

I

L

M

D

Q

L

L

F

K

E

K

L

L

N

N

E

E

K

E

K

D

E

G

R

K

T

T

I

V

V

V

M

V

V

V

L

T

H
|
H

D

D

L

I

N

N

L

V

A

A

S

-

R

R

Y

F

A

G

H

E

N

R

I

I

A

Y

I

L

K

K

N

D

G

G

E

E

V

V

H

E

A

R

Q

E

G

E

K

K

binding adenosine-5'-triphosphate: Y11 (≠ L10), S40 (≠ N39), G41 (= G40), S42 (≠ C41), G43 (= G42), K44 (= K43), S45 (= S44), T46 (= T45), F138 (≠ A137), Q145 (≠ T140), S147 (= S142), G149 (= G144), Q150 (= Q145), H204 (= H199)

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
30% identity, 82% coverage: 5:228/274 of query aligns to 3:226/253 of 6z5uK

query
sites
6z5uK

V

I

R

E

T

V

E

K

S

N

L

L

S

S

I

F

G

N

Y
x
R

T

G

D

E

H

R

L

V

L

I

F

Y

E

D

N

N

L

I

D

S

L

L

H

N

I

I

P

R

R

R

G

G

E

Q

I

I

S

T

V

A

F

I

V

M

G

G

S

P

N
x
S

G
|
G

C

T

G
|
G

K
|
K

S
x
T

T
|
T

L

L

R

R

S

L

I

I

A

G

R

G

L

Q

L

L

K

V

P

P

T

D

T

Q

G

G

S

E

V

V

L

L

E

D

G

G

K

K

D

D

V

I

H

A

H

Q

M

M

S

S

S

R

R

Q

D

E

V

L

A

F

-

A

-

R

K

A

K

R

M

M

G

G

I

M

L

L

P

F

Q
|
Q

S

S

P

G

V

A

S

L

P

F

E

T

G

D

L

M

T

S

V

V

H

Y

E

E

L

N

V

V

K

A

Q

-

G

-

R

-

Y

F

P

P

H

I

Q

R

T

A

W

H

L

T

K

K

R

L

W

S

S

E

A

N

E

L

D

I

T

A

E

E

K

L

V

V

E

A

A

L

A

K

M

L

E

E

A

S

T

V

R

G

I

L

S

R

D

G

L

T

R

E

D

Q

R

L

A

M

V

P

D

T

T

E

L

L

S

S

G

G

Q

M

R

N

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

T

A

L

I

A

A

Q

L

D

D

T

P

D

D

V

L

I

I

L

M

L

Y

D

D

E
|
E

P

P

T

F

T

A

Y

G

L

Q

D

D

M

P

T

I

H

V

Q

K

I

G

E

V

I

L

L

T

D

R

L

L

L

I

F

R

E

S

L

L

N

R

E

E

K

A

K

L

E

D

R

L

T

T

I

T

V

I

M

I

V

V

L

S

H
|
H

D

D

L

V

N

P

L

E

A

T

S

L

R

S

Y

I

A

A

H

D

N

Y

I

I

I

Y

A

V

I

V

K

A

N

E

G

G

E

K

V

I

H

Q

A

G

Q

E

G

G

K

T

P

P

E

E

N

E

I

L

binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ Y14), S37 (≠ N39), G38 (= G40), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45), Q86 (= Q85), E164 (= E166), H197 (= H199)
binding magnesium ion: Q86 (= Q85), E164 (= E166)

8g4cB Bceabs atpgs high res tm (see paper)
31% identity, 74% coverage: 19:222/274 of query aligns to 22:224/248 of 8g4cB

query
sites
8g4cB

L

V

F

L

E

K

N

G

L

I

D

D

L

I

H

H

I

I

P

E

R

K

G

G

E

E

I

F

S

V

V

S

F

I

V

M

G

G

S

A

N
x
S

G

G

C
x
S

G

G

K

K

S
x
T

T

T

L

L

R

N

S

V

I

L

A

S

R

S

L

I

L

D

K

Q

P

V

T

S

T

H

G

G

S

T

V

I

L

H

L

I

E

N

G

G

K

N

D

D

V

M

H

T

H

A

M

M

S

K

S

E

R

K

D

Q

V

L

A

A

-

E

-

F

-

R

-

K

K

Q

K

H

M

L

G

G

I

F

L

I

P

F

Q

Q

S

D

P

Y

V

N

S

L

P

L

E

D

G

T

L

L

T

T

V

V

H

K

E

E

L

N

V

I

K

-

Q

-

G

-

R

L

Y

L

P

P

H

-

Q

L

T

S

W

I

L

T

K

K

R

L

W

S

S

K

A

K

E

E

D

A

T

N

E

R

K

K

V

F

E

E

A

E

A

V

M

A

E

K

A

E

T

L

R

G

I

I

S

Y

D

E

L

L

R

R

D

D

R

K

A

Y

V

P

D

N

T

E

L

I

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

T

W

S

I

A

A

G

M

R

T

A

L

F

A

I

Q

H

D

D

T

P

D

S

V

I

I

I

L

F

L

A

D
|
D

E

E

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

S

Q

A

I

S

E

D

I

L

L

L

D

N

L

K

L

L

F

S

E

Q

L

L

N

N

E

Q

K

K

K

R

E

N

R

A

T

T

I

I

V

I

M

M

V

V

L

T

H

H

D

D

L

-

N

P

L

V

A

A

S

A

R

S

Y

Y

A

C

H

G

N

R

I

V

I

I

A

F

I

I

K

K

N

D

G

G

E

Q

V

M

H

Y

A

T

Q

Q

binding phosphothiophosphoric acid-adenylate ester: S42 (≠ N39), S44 (≠ C41), T47 (≠ S44), D168 (= D165)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

7tchB Bceab e169q variant atp-bound conformation (see paper)
30% identity, 74% coverage: 19:222/274 of query aligns to 21:223/245 of 7tchB

query
sites
7tchB

L
x
V

F

L

E

K

N

G

L

I

D

D

L

I

H

H

I

I

P

E

R

K

G

G

E

E

I

F

S

V

V

S

F

I

V

M

G

G

S

A

N
x
S

G
|
G

C
x
S

G

G

K
|
K

S
x
T

T

T

L

L

R

N

S

V

I

L

A

S

R

S

L

I

L

D

K

Q

P

V

T

S

T

H

G

G

S

T

V

I

L

H

L

I

E

N

G

G

K

N

D

D

V

M

H

T

H

A

M

M

S

K

S

E

R

K

D

Q

V

L

A

A

-

E

-

F

-

R

-

K

K

Q

K

H

M

L

G

G

I

F

L

I

P

F

Q

Q

S

D

P

Y

V

N

S

L

P

L

E

D

G

T

L

L

T

T

V

V

H

K

E

E

L

N

V

I

K

-

Q

-

G

-

R

L

Y

L

P

P

H

-

Q

L

T

S

W

I

L

T

K

K

R

L

W

S

S

K

A

K

E

E

D

A

T

N

E

R

K

K

V

F

E

E

A

E

A

V

M

A

E

K

A

E

T

L

R

G

I

I

S

Y

D

E

L

L

R

R

D

D

R

K

A

Y

V

P

D

N

T
x
E

L

I

S

S

G
|
G

Q

Q

R

K

Q

Q

R

R

A

T

W

S

I

A

A

G

M

R

T

A

L

F

A

I

Q

H

D

D

T

P

D

S

V

I

I

I

L

F

L

A

D

D

E

Q

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

S

Q

A

I

S

E

D

I

L

L

L

D

N

L

K

L

L

F

S

E

Q

L

L

N

N

E

Q

K

K

K

R

E

N

R

A

T

T

I

I

V

I

M

M

V

V

L

T

H

H

D

D

L

-

N

P

L

V

A

A

S

A

R

S

Y

Y

A

C

H

G

N

R

I

V

I

I

A

F

I

I

K

K

N

D

G

G

E

Q

V

M

H

Y

A

T

Q

Q

binding adenosine-5'-triphosphate: V21 (≠ L19), S41 (≠ N39), G42 (= G40), S43 (≠ C41), K45 (= K43), T46 (≠ S44), E142 (≠ T140), G145 (= G143), G146 (= G144)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 19

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
32% identity, 81% coverage: 3:225/274 of query aligns to 1:217/374 of 2awnB

query
sites
2awnB

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

L
x
V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N
x
S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q

Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V

V

E

N

A

Q

A

V

M

A

E

E

A

V

T

L

R

Q

I

L

S

A

D

H

L

L

R

L

D

D

R

R

A

K

V

P

D

K

T

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H

H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y14), V17 (≠ L19), S37 (≠ N39), G38 (= G40), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 81% coverage: 3:225/274 of query aligns to 1:217/371 of 3puyA

query
sites
3puyA

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q
|
Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V

V

E

N

A

Q

A

V

M

A

E

E

A

V

T

L

R

Q

I

L

S

A

D

H

L

L

R

L

D

D

R
|
R

A

K

V

P

D

K

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y14), S37 (≠ N39), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), Q81 (= Q85), R128 (= R136), A132 (≠ T140), S134 (= S142), G136 (= G144), Q137 (= Q145), E158 (= E166), H191 (= H199)
binding magnesium ion: S42 (= S44), Q81 (= Q85)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 81% coverage: 3:225/274 of query aligns to 1:217/371 of 3puxA

query
sites
3puxA

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q
|
Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V

V

E

N

A

Q

A

V

M

A

E

E

A

V

T

L

R

Q

I

L

S

A

D

H

L

L

R

L

D

D

R
|
R

A

K

V

P

D

K

T

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y14), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R136), S134 (= S142), Q137 (= Q145)
binding beryllium trifluoride ion: S37 (≠ N39), G38 (= G40), K41 (= K43), Q81 (= Q85), S134 (= S142), G136 (= G144), H191 (= H199)
binding magnesium ion: S42 (= S44), Q81 (= Q85)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
32% identity, 81% coverage: 3:225/274 of query aligns to 1:217/371 of 3puwA

query
sites
3puwA

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

L
x
V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q
|
Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V

V

E

N

A

Q

A

V

M

A

E

E

A

V

T

L

R

Q

I

L

S

A

D

H

L

L

R

L

D

D

R
|
R

A

K

V

P

D

K

T
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y14), V17 (≠ L19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R136), A132 (≠ T140), S134 (= S142), Q137 (= Q145)
binding tetrafluoroaluminate ion: S37 (≠ N39), G38 (= G40), K41 (= K43), Q81 (= Q85), S134 (= S142), G135 (= G143), G136 (= G144), E158 (= E166), H191 (= H199)
binding magnesium ion: S42 (= S44), Q81 (= Q85)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
32% identity, 81% coverage: 3:225/274 of query aligns to 1:217/371 of 3puvA

query
sites
3puvA

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

L
x
V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q
|
Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V

V

E

N

A

Q

A

V

M

A

E

E

A

V

T

L

R

Q

I

L

S

A

D

H

L

L

R

L

D

D

R
|
R

A

K

V

P

D

K

T
x
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H

H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y14), V17 (≠ L19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R136), A132 (≠ T140), S134 (= S142), Q137 (= Q145)
binding magnesium ion: S42 (= S44), Q81 (= Q85)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 81% coverage: 3:225/274 of query aligns to 2:218/371 of P68187

query
sites
P68187

T

A

A

S

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y

W

T

G

D

E

H

V

L

V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q

Q

S

S

P

Y

V
x
A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E
x
K

D

K

-

E

-

V

-

I

T

N

E

Q

K

R

V
|
V

E

N

A

Q

A
x
V

M

A

E
|
E

A

V

T

L

R

Q

I

L

S
x
A

D

H

L

L

R

L

D

D

R

R

A

K

V

P

D

K

T

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

L

L

A

V

Q

A

D

E

T

P

D

S

V

V

I

F

L

L

D
|
D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

M

A

T

A

H

L

Q

R

I

V

E

Q

I

M

L

R

D

I

L

E

L

I

F

S

E

R

L

L

N

H

E

K

K

R

K

L

E

G

R

R

T

T

I

M

V

I

M

Y

V

V

L

T

H

H

D

D

L

Q

N

V

L

E

A

A

S

M

R

T

Y

L

A

A

H

D

N

K

I

I

I

V

A

V

I

L

K

D

N

A

G

G

E

R

V

V

H

A

A

Q

Q

V

G

G

K

K

P

P

A85 (≠ V88) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ E116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V121) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A124) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E126) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ S131) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G144) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D165) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
30% identity, 84% coverage: 4:232/274 of query aligns to 3:231/262 of 7chaI

query
sites
7chaI

A

A

V

V

R

E

T

L

E

K

S

G

L

L

S

T

I

F

G

K

Y
x
R

T

G

D

S

H

R

L

A

L

I

F

F

E

D

N

N

L

I

D

D

L

V

H

R

I

I

P

P

R

R

G

G

E

K

I

V

S

T

V

G

F

I

V

M

G

G

S

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

R

R

S

L

I

I

A

A

R

S

L

Q

L

L

K

R

P

P

T

S

T

K

G

G

S

E

V

V

L

W

L

V

E

N

G

G

K

Q

D

N

V

L

H

P

H

Q

M

L

S

S

S

R

R

G

D

D

V

L

A

F

-

D

-

M

-

R

K

K

K

Q

M

F

G

G

I

V

L

L

P

F

Q

Q

S

S

P

G

V

A

S

L

P

F

E

T

G

D

L

L

T

D

V

V

H

F

E

E

L

N

V

V

K

A

Q

-

G

-

R

-

Y

F

P

P

H

L

Q

R

T

V

W

H

L

T

K

Q

R

L

W

P

S

E

A

E

E

M

D

I

T

R

E

D

K

I

V

V

E

L

A

M

A

K

M

L

E

Q

A

A

T

V

R

G

I

L

S

R

D

G

L

A

R

V

D

E

R

L

A

M

V

P

D

D

T

E

L

L

S

S

G

G

Q

M

R

K

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

T

A

L

I

A

A

Q

L

D

D

T

P

D

Q

V

I

I

L

L

L

L

Y

D

D

E

E

P

P

T

F

T

V

Y

G

L

Q

D

D

M

P

T

I

H

A

Q

M

I

G

E

V

I

L

L

V

D

R

L

L

L

I

F

R

E

L

L

L

N

N

E

D

K

A

K

L

E

G

R

I

T

T

I

S

V

I

M

V

V

V

L

S

H

H

D

D

L

L

N

A

L

E

A

T

S

A

R

S

Y

I

A

A

H

D

N

Y

I

I

I

Y

A

I

I

V

K

G

N

D

G

G

E

R

V

V

H

L

A

G

Q

H

G

G

K

T

P

P

E

D

N

V

I

L

I

K

T

E

C

T

D

D

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ Y14), S38 (≠ N39), G39 (= G40), C40 (= C41), G41 (= G42), K42 (= K43), T43 (≠ S44), T44 (= T45)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
32% identity, 81% coverage: 5:225/274 of query aligns to 1:215/367 of 1q12A

query
sites
1q12A

V

V

R

Q

T

L

E

Q

S

N

L

V

S

T

I

K

G

A

Y
x
W

T

G

D

E

H

V

L

V

F

S

E

K

N

D

L

I

D

N

L

L

H

D

I

I

P

H

R

E

G

G

E

E

I

F

S

V

V

V

F

F

V

V

G

G

S

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

M

I

I

A

A

R

G

L

L

L

E

K

T

P

I

T

T

T

S

G

G

S

D

V

L

L

F

L

I

E

G

G

E

K

K

D

R

V

M

H

N

H

D

M

T

S

P

R

A

D

E

V

-

A

-

K

R

K

G

M

V

G

G

I

M

L

V

P

F

Q

Q

S

S

P

Y

V

A

S

L

P

Y

E

P

G

H

L

L

T

S

V

V

H

A

E

E

L

N

V

M

K

S

Q

F

G

G

R

-

Y

-

P

-

H

-

Q

-

T

-

W

-

L

L

K

K

R

L

W

A

S

G

A

A

E

K

D

K

-

E

-

V

-

I

T

N