SitesBLAST
Comparing WP_075858559.1 NCBI__GCF_001950255.1:WP_075858559.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
42% identity, 94% coverage: 33:525/525 of query aligns to 33:525/526 of 3dc2A
- active site: N96 (= N96), R230 (= R230), D254 (= D254), E259 (= E259), H277 (= H277)
- binding serine: Y458 (≠ H458), D460 (= D460), R461 (≠ Q461), P462 (= P462), G463 (≠ L463), A464 (≠ M464), L465 (≠ I465), L484 (= L484)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
42% identity, 94% coverage: 33:525/525 of query aligns to 34:524/525 of 3ddnB
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
45% identity, 75% coverage: 2:397/525 of query aligns to 8:404/533 of O43175
- T78 (≠ V72) binding NAD(+)
- R135 (= R129) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 149:150) binding NAD(+)
- D175 (= D169) binding NAD(+)
- T207 (≠ L201) binding NAD(+)
- CAR 234:236 (= CAR 228:230) binding NAD(+)
- D260 (= D254) binding NAD(+)
- V261 (= V255) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (= HLGA 277:280) binding NAD(+)
- A373 (= A367) to T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- G377 (= G371) to S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
50% identity, 58% coverage: 2:303/525 of query aligns to 4:305/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
50% identity, 57% coverage: 2:302/525 of query aligns to 3:303/303 of 6plgA
7dkmA Phgdh covalently linked to oridonin (see paper)
50% identity, 57% coverage: 2:302/525 of query aligns to 4:304/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V72), A102 (= A100), G148 (= G146), R151 (= R149), I152 (= I150), Y170 (= Y168), D171 (= D169), P172 (= P170), I173 (≠ F171), H202 (= H200), T203 (≠ L201), P204 (= P202), T209 (= T207), C230 (= C228), A231 (= A229), R232 (= R230), H279 (= H277), G281 (= G279)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ K12), K17 (≠ N15), I18 (= I16), E293 (≠ I291)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
50% identity, 57% coverage: 2:301/525 of query aligns to 3:302/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I145), G147 (= G146), L148 (= L147), G149 (= G148), R150 (= R149), I151 (= I150), G152 (= G151), D170 (= D169), H201 (= H200), T202 (≠ L201), P203 (= P202)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
50% identity, 57% coverage: 2:301/525 of query aligns to 3:302/302 of 6rihA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
50% identity, 57% coverage: 2:300/525 of query aligns to 3:301/301 of 6rj5A
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
50% identity, 57% coverage: 2:299/525 of query aligns to 2:299/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N96), A100 (= A100), R149 (= R149), I150 (= I150), Y168 (= Y168), D169 (= D169), P170 (= P170), I171 (≠ F171), H200 (= H200), T201 (≠ L201), P202 (= P202), T207 (= T207), C228 (= C228), A229 (= A229), R230 (= R230), H277 (= H277), G279 (= G279)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
51% identity, 56% coverage: 2:294/525 of query aligns to 2:294/297 of 6rj3A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
50% identity, 57% coverage: 3:301/525 of query aligns to 1:299/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G148), I148 (= I150), Y166 (= Y168), D167 (= D169), P168 (= P170), I169 (≠ F171), I170 (= I172), H198 (= H200), T199 (≠ L201), L208 (= L210), R228 (= R230)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
48% identity, 57% coverage: 2:300/525 of query aligns to 2:292/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R149), Y160 (= Y168), D161 (= D169), P162 (= P170), I164 (= I172), L179 (= L187), T193 (≠ L201), P194 (= P202), S198 (≠ E206), L202 (= L210)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
47% identity, 58% coverage: 1:303/525 of query aligns to 1:304/304 of 1wwkA