SitesBLAST
Comparing WP_075858773.1 NCBI__GCF_001950255.1:WP_075858773.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
65% identity, 99% coverage: 2:348/349 of query aligns to 1:348/350 of 2zsjA
- active site: K61 (= K60), T85 (= T84), Q218 (≠ E217), A222 (= A221), A240 (= A239), T317 (= T316)
- binding pyridoxal-5'-phosphate: F60 (= F59), K61 (= K60), N87 (= N86), V186 (= V185), G187 (= G186), N188 (= N187), A189 (= A188), G190 (= G189), N191 (= N190), A240 (= A239), T317 (= T316), G318 (= G317)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
62% identity, 99% coverage: 3:348/349 of query aligns to 1:345/345 of 6cgqB
- active site: K58 (= K60), T82 (= T84), E214 (= E217), S218 (≠ A221), A236 (= A239), T313 (= T316)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K60), S81 (= S83), T82 (= T84), N84 (= N86), T85 (= T87), V183 (= V185), G184 (= G186), N185 (= N187), A186 (= A188), N188 (= N190), A236 (= A239), I237 (= I240), E283 (= E286), T313 (= T316)
- binding phosphate ion: K58 (= K60), T85 (= T87), N151 (= N153), S152 (= S154), R157 (= R159), N185 (= N187)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
62% identity, 99% coverage: 3:348/349 of query aligns to 3:347/350 of 6nmxA
- active site: K60 (= K60), T84 (= T84), E216 (= E217), S220 (≠ A221), A238 (= A239), T315 (= T316)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K60), S83 (= S83), T84 (= T84), N86 (= N86), T87 (= T87), F133 (= F133), N153 (= N153), S154 (= S154), R159 (= R159), V185 (= V185), G186 (= G186), N187 (= N187), A188 (= A188), G189 (= G189), N190 (= N190), A238 (= A239), I239 (= I240), E285 (= E286), T315 (= T316)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
61% identity, 99% coverage: 5:348/349 of query aligns to 1:337/339 of 6cgqA
- active site: K56 (= K60), T80 (= T84), E206 (= E217), S210 (≠ A221), A228 (= A239), T305 (= T316)
- binding pyridoxal-5'-phosphate: F55 (= F59), K56 (= K60), N82 (= N86), V175 (= V185), G176 (= G186), N177 (= N187), A178 (= A188), G179 (= G189), N180 (= N190), A228 (= A239), E275 (= E286), T305 (= T316), G306 (= G317)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
60% identity, 92% coverage: 6:326/349 of query aligns to 4:326/350 of 3aeyA
- active site: K60 (= K60), T84 (= T84), P211 (= P211), G215 (= G215), Q217 (≠ E217), A239 (= A239), T316 (= T316)
- binding sulfate ion: K60 (= K60), K60 (= K60), G85 (= G85), N86 (= N86), T87 (= T87), T87 (= T87), S154 (= S154), R159 (= R159), N187 (= N187), R228 (≠ E228), V230 (≠ I230), E231 (≠ S231), R232 (≠ N232), A239 (= A239)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
60% identity, 92% coverage: 6:326/349 of query aligns to 5:327/350 of 1uimA
- active site: K61 (= K60), T85 (= T84), P212 (= P211), G216 (= G215), Q218 (≠ E217), A240 (= A239), T317 (= T316)
- binding pyridoxal-5'-phosphate: F60 (= F59), K61 (= K60), N87 (= N86), G187 (= G186), N188 (= N187), A189 (= A188), G190 (= G189), N191 (= N190), A240 (= A239), E287 (= E286), T317 (= T316), G318 (= G317)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
60% identity, 92% coverage: 6:326/349 of query aligns to 5:327/351 of 3aexA
- active site: K61 (= K60), T85 (= T84), P212 (= P211), G216 (= G215), Q218 (≠ E217), A240 (= A239), T317 (= T316)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K60), S84 (= S83), T85 (= T84), N87 (= N86), T88 (= T87), V186 (= V185), G187 (= G186), N188 (= N187), A189 (= A188), G190 (= G189), N191 (= N190), A240 (= A239), I241 (= I240), E287 (= E286), T317 (= T316)
- binding phosphate ion: K61 (= K60), T88 (= T87), N154 (= N153), S155 (= S154), R160 (= R159), N188 (= N187)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
60% identity, 92% coverage: 6:326/349 of query aligns to 5:327/351 of 1v7cA
- active site: K61 (= K60), T85 (= T84), P212 (= P211), G216 (= G215), Q218 (≠ E217), A240 (= A239), T317 (= T316)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K60), S84 (= S83), T85 (= T84), N87 (= N86), T88 (= T87), F134 (= F133), N154 (= N153), S155 (= S154), R160 (= R159), V186 (= V185), G187 (= G186), N188 (= N187), A189 (= A188), G190 (= G189), N191 (= N190), A240 (= A239), I241 (= I240), E287 (= E286), T317 (= T316)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
58% identity, 97% coverage: 3:339/349 of query aligns to 12:350/360 of A0R220
- K151 (≠ A142) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
59% identity, 94% coverage: 3:329/349 of query aligns to 12:339/360 of P9WG59
- K69 (= K60) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N86) binding pyridoxal 5'-phosphate
- K151 (≠ A142) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 186:190) binding pyridoxal 5'-phosphate
- T326 (= T316) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
59% identity, 94% coverage: 3:329/349 of query aligns to 3:330/349 of 2d1fA
- active site: K60 (= K60), T84 (= T84), D209 (≠ K208), R213 (≠ K212), L215 (≠ M214), A240 (= A239), T317 (= T316)
- binding pyridoxal-5'-phosphate: F59 (= F59), K60 (= K60), N86 (= N86), V186 (= V185), G187 (= G186), N188 (= N187), A189 (= A188), G190 (= G189), N191 (= N190), A240 (= A239), T317 (= T316)
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
33% identity, 99% coverage: 4:348/349 of query aligns to 70:440/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F59), K128 (= K60), D159 (≠ N86), G259 (≠ V185), G260 (= G186), N261 (= N187), L262 (≠ A188), G263 (= G189), N264 (= N190), A321 (= A239), H369 (≠ A288), T397 (= T316)
- binding s-adenosylmethionine: S90 (≠ T24), F92 (≠ N26), N97 (≠ P31), L98 (= L32), W100 (≠ Y34), W115 (≠ Y47), W115 (≠ Y47), Q246 (≠ A173), F247 (≠ L174)
Sites not aligning to the query:
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
33% identity, 99% coverage: 4:348/349 of query aligns to 145:515/526 of Q9S7B5
- N172 (≠ P31) binding S-adenosyl-L-methionine
- L173 (= L32) binding S-adenosyl-L-methionine
- K181 (≠ A44) binding in monomer B; binding in monomer A
- N187 (vs. gap) binding in monomer B
- L205 (≠ R62) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
33% identity, 99% coverage: 5:348/349 of query aligns to 89:442/448 of 2c2gA
8y1jA Structure of the pyridoxal 5'-phosphate-dependent (plp) threonine deaminase ilva1 from pseudomonas aeruginosa pao1 (see paper)
29% identity, 78% coverage: 29:299/349 of query aligns to 19:288/502 of 8y1jA
Sites not aligning to the query:
Q8VBT2 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Mus musculus (Mouse)
27% identity, 81% coverage: 29:309/349 of query aligns to 10:288/327 of Q8VBT2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
1pwhA Rat liver l-serine dehydratase- complex with pyridoxyl-(o-methyl- serine)-5-monophosphate (see paper)
27% identity, 77% coverage: 29:296/349 of query aligns to 10:277/327 of 1pwhA
- active site: K41 (= K60), A65 (≠ T84), E194 (= E217), A198 (= A221), S200 (vs. gap), A222 (≠ R241), A269 (= A288)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-o-methyl-l-serine: F40 (= F59), K41 (= K60), S64 (= S83), A65 (≠ T84), N67 (= N86), A68 (≠ T87), F136 (≠ S154), G168 (= G186), G169 (≠ N187), G170 (≠ A188), G171 (= G189), A222 (≠ R241), G224 (= G243)
Sites not aligning to the query:
8egzB Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate
29% identity, 89% coverage: 30:338/349 of query aligns to 52:385/386 of 8egzB
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H81 (≠ F59), K82 (= K60), T105 (≠ A82), G106 (≠ S83), A107 (≠ T84), Q109 (≠ N86), H110 (≠ T87), S185 (≠ A150), G229 (≠ A188), S230 (≠ G189), N231 (= N190), G297 (≠ A254), E344 (≠ A288), S367 (≠ T316)
8eh0A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with quinoline n-oxide
29% identity, 89% coverage: 30:338/349 of query aligns to 51:384/385 of 8eh0A
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H80 (≠ F59), K81 (= K60), T104 (≠ A82), G105 (≠ S83), A106 (≠ T84), Q108 (≠ N86), H109 (≠ T87), S184 (≠ A150), G228 (≠ A188), S229 (≠ G189), N230 (= N190), G296 (≠ A254), E343 (≠ A288), S366 (≠ T316), G367 (= G317)
- binding 1-oxo-1lambda~5~-quinoline: L160 (vs. gap), I164 (≠ F133), Y180 (vs. gap), P182 (= P148), G183 (≠ I149), S184 (≠ A150), V186 (= V152), Y299 (≠ E257)
8egyA Engineered holo tyrosine synthase (tmtyrs1) derived from t. Maritima trpb
29% identity, 89% coverage: 30:338/349 of query aligns to 51:384/385 of 8egyA
Query Sequence
>WP_075858773.1 NCBI__GCF_001950255.1:WP_075858773.1
MRWEGVIKAYREYLPVTEKTPLLTLNEGNTPLIYASYLSERVGARVYLKFEGANPTGSFK
DRGMVVAVAKAIEEGARAVICASTGNTSAAAAAYAAKAGLKCVVLIPEGNIALGKLAQAL
FYGAKVVAIKGNFDEALNLVRAIGQNYPIAIVNSINPYRIEGQKTASFEVADALGDAPDY
LFIPVGNAGNITAYWQGFKQYYELGKTKKLPKMMGFEAQGAAAIVKGEPISNPETIATAI
RIGNPASWQGAVAAANESGGKISYVTDEEIIEAYRLLAQKEGIMAEPASAASVAGLLKYR
RKHEFNQDDVVVLVLTGHGLKDPDNALKQNANQPVVVEPDYNEVLKIIL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory