SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_075858796.1 NCBI__GCF_001950255.1:WP_075858796.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

1dbfA Chorismate mutase from bacillus subtilis at 1.30 angstrom (see paper)
41% identity, 100% coverage: 1:117/117 of query aligns to 2:120/127 of 1dbfA

query
sites
1dbfA
M
 
M
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I
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R
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G
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A
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E
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E
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E
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K
E
 
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L
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L
A
 
E
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E
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E
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E
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P
D
 
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S

Sites not aligning to the query:

P19080 Chorismate mutase AroH; CM; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see 2 papers)
40% identity, 100% coverage: 1:117/117 of query aligns to 2:120/127 of P19080

query
sites
P19080
M
 
M
L
 
I
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R
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G
I
 
I
R
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R
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G
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A
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T
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E
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R
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D
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T
P
 
E
D
 
E
A
 
E
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I
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L
E
 
Q
A
 
K
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T
V
 
K
E
 
Q
L
 
L
L
 
L
A
 
E
A
 
K
I
 
I
F
 
I
Q
 
E
E
 
E
N
 
N
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H
L
 
T
S
 
K
R
 
P
E
 
E
K
 
D
I
 
V
T
 
V
A
 
Q
V
 
M
F
 
L
F
 
L
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S
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A
T
 
T
A
 
P
D
 
D
L
 
L
V
 
H
T
 
A
A
 
V
Y
 
F
P
 
P
A
 
A
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K
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A
A
 
V
R
 
R
E
 
E
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L
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Y
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x
M
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M
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L
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R
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P
D
 
D
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L
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S

3zp7A Arg90cit chorismate mutase of bacillus subtilis in complex with chorismate and prephenate (see paper)
40% identity, 97% coverage: 1:113/117 of query aligns to 2:116/117 of 3zp7A

query
sites
3zp7A
M
 
M
L
 
I
K
 
R
G
 
G
I
 
I
R
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R
G
 
G
A
 
A
I
 
T
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V
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E
R
 
R
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D
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T
P
 
E
D
 
E
A
 
E
I
 
I
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L
E
 
Q
A
 
K
T
 
T
V
 
K
E
 
Q
L
 
L
L
 
L
A
 
E
A
 
K
I
 
I
F
 
I
Q
 
E
E
 
E
N
 
N
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H
L
 
T
S
 
K
R
 
P
E
 
E
K
 
D
I
 
V
T
 
V
A
 
Q
V
 
M
F
 
L
F
 
L
T
 
S
Q
 
A
T
 
T
A
 
P
D
 
D
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L
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H
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A
A
 
V
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x
F
P
 
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A
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V
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E
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L
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M
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R

2chtA Crystal structures of the monofunctional chorismate mutase from bacillus subtilis and its complex with a transition state analog (see paper)
40% identity, 96% coverage: 1:112/117 of query aligns to 1:114/114 of 2chtA

query
sites
2chtA
M
 
M
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I
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R
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I
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G
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A
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E
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D
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E
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E
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E
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I
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A
 
K
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T
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K
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Q
L
 
L
L
 
L
A
 
E
A
 
K
I
 
I
F
 
I
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E
E
 
E
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N
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H
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E
 
E
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D
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V
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M
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A
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P
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D
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A
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A
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M
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P
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E
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K
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A
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V
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L

1comA The monofunctional chorismate mutase from bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications on the mechanism of enzymatic reaction (see paper)
40% identity, 96% coverage: 1:112/117 of query aligns to 1:114/114 of 1comA

query
sites
1comA
M
 
M
L
 
I
K
 
R
G
 
G
I
 
I
R
|
R
G
 
G
A
 
A
I
 
T
S
 
T
V
 
V
E
 
E
R
 
R
D
 
D
T
 
T
P
 
E
D
 
E
A
 
E
I
 
I
K
 
L
E
 
Q
A
 
K
T
 
T
V
 
K
E
 
Q
L
 
L
L
 
L
A
 
E
A
 
K
I
 
I
F
 
I
Q
 
E
E
 
E
N
 
N
K
 
H
L
 
T
S
 
K
R
 
P
E
 
E
K
 
D
I
 
V
T
 
V
A
 
Q
V
 
M
F
 
L
F
 
L
T
 
S
Q
 
A
T
 
T
A
 
P
D
 
D
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L
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H
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A
A
 
V
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x
F
P
 
P
A
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A
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K
F
 
A
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V
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R
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E
F
 
L
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S
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G
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W
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Q
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Y
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P
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M
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M
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M
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D
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V
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G
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G
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L
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K
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C
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I
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V
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M
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P
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D
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Q
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I
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H
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V
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Y
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L
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E
E
 
K
A
 
A
L
 
V
K
 
V
L
 
L

Query Sequence

>WP_075858796.1 NCBI__GCF_001950255.1:WP_075858796.1
MLKGIRGAISVERDTPDAIKEATVELLAAIFQENKLSREKITAVFFTQTADLVTAYPAKF
AREFGLKDVPLMSAQEPNVVNSLPRVIRVLILAQVNEGDGIKHVYLKEALKLRRDLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory