SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_075859204.1 NCBI__GCF_001950255.1:WP_075859204.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
71% identity, 100% coverage: 1:306/306 of query aligns to 1:306/310 of P9WP55

query
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P9WP55

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K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S266) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
71% identity, 100% coverage: 1:306/306 of query aligns to 1:306/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), N74 (= N74), T75 (= T75), Q144 (= Q144), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), I223 (= I223), S266 (= S266), P293 (= P293), D294 (= D294)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
70% identity, 98% coverage: 1:300/306 of query aligns to 1:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), I126 (≠ V126), Q144 (= Q144), F145 (= F145), K215 (≠ P215), G222 (= G222), A225 (= A225), F227 (= F227)
binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), S266 (= S266), P293 (= P293), D294 (= D294)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
70% identity, 98% coverage: 1:300/306 of query aligns to 1:300/300 of 2q3cA

query
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2q3cA

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active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding : T71 (= T71), S72 (= S72), G73 (= G73), T75 (= T75), M122 (= M122), Q144 (= Q144), K215 (≠ P215), G222 (= G222), A225 (= A225)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
61% identity, 100% coverage: 1:306/306 of query aligns to 1:309/318 of 4lmaA

query
sites
4lmaA

M

M

R

R

I

I

Y

A

Q

H

D

D

I

V

T

T

E

E

L

L

I

V

G

G

K

R

T

T

P

P

L

L

L

V

K

Q

L

L

K

N

R

R

V

I

-

P

-

Q

S

A

K

E

D

G

A

C

K

L

A

G

T

R

V

I

V

V

A

M

K

K

L

L

E

E

Y

G

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

Y

T

N

H

M

M

I

I

K

N

A

S

A

A

E

E

E

K

K

A

G

G

L

L

L

I

D

N

K

P

D

E

T

T

-

T

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

V

T

A

A

R

K

G

G

Y

Y

R

R

L

L

I

I

I

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

M

A

L

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

T

I

L

V

E

L

L

T

T

P

P

G

G

E

S

L

Q

G

G

M

M

T

K

G

G

A

A

V

I

N

L

K

R

A

A

L

Q

E

Q

L

I

A

V

K

D

E

S

Y

I

P

P

K

G

S

A

F

Y

I

M

P

L

Q

Q

F

F

E

R

N

N

P

P

A

S

N

N

P

P

E

E

I

I

H

H

R

R

Q

L

T

T

A

A

L

E

E

E

I

I

W

W

E

Q

D

D

T

T

D

E

G

G

K

Q

V

V

D

D

I

F

V

I

V

V

G

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

G

A

E

E

V

V

L

I

K

K

A

S

K

R

K

K

P

P

E

S

V

F

K

Q

I

V

A

A

V

V

E

E

P

P

A

F

A

N

S

S

P

P

V

V

L

I

S

S

G

G

K

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

E

V

V

L

L

N

R

L

T

D

D

V

L

I

I

D

D

E

E

I

V

I

I

R

T

V

V

E

S

N

D

E

E

D

E

A

A

F

F

A

Q

T

F

A

G

K

R

E

R

L

L

A

A

R

K

E

E

G

G

V

L

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

A

N

L

L

W

C

A

A

L

I

E

Q

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

E

E

G

G

K

K

L

L

I

I

V

V

V

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

L

L

M

F

F

S

Q

active site: K46 (= K44), S269 (= S266)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
63% identity, 100% coverage: 1:305/306 of query aligns to 1:308/310 of 4lmbA

query
sites
4lmbA

M

M

R

L

I

I

Y

A

Q

R

D

D

I

I

T

T

E

Q

L

L

I

V

G

G

K

R

T

T

P

P

L

L

L

V

K

Q

L

L

K

N

R

R

-

I

-

P

V

V

S

A

K

E

D

G

A

V

K

K

A

A

T

R

V

I

V

V

A

V

K

K

L

L

E

E

Y

S

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

Y

V

N

S

M

M

I

V

K

E

A

D

A

A

E

E

E

A

K

A

G

G

L

L

L

I

D

H

K

P

D

D

-

K

T

T

V

I

I

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

V

V

A

A

R

K

G

G

Y

Y

R

R

L

L

I

V

I

L

T

T

M

M

P

P

E

E

T

T

M
|
M

S

S

V

L

E

E

R

R

M

A

L

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

Q

I

L

V

E

L

L

T

T

P

P

G

G

E

S

L

Q

G

G

M
|
M

T

E

G

G

A

A

V

I

N

T

K

R

A

A

L

E

E

E

L

I

A

V

K

E

E

N

Y

T

P

P

K

N

S

A

F

Y

I

S

P

L

Q

Q

Q
|
Q

F
|
F

E

R

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

R

Q

E

T

T

A

A

L

E

E

E

I

I

W

W

E

A

D

D

T

T

D

D

G

G

K

L

V

V

D

D

I

I

V

V

V

I

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

G

A

E

E

V

T

L

I

K

K

A

P

K

R

P

P

E

Q

V

F

K

Q

I

A

I

I

A

A

V

V

E

E

P

P

A

S

A

N

S

S

P

P

V

V

L

L

S

S

G

G

K

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

K

A

V

I

L

F

N

R

L

P

D

E

V

L

I

I

D

D

E

E

I

V

I

I

R

I

V

V

E

D

N

D

E

T

D

E

A

A

F

F

A

A

T

Y

A

A

K

R

E

R

L

L

A

A

R

R

E

Q

E

E

G

G

V

L

L

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

L

L

W

W

A

A

L

I

E

Q

V

V

A

G

K

K

R

R

P

P

E

E

N

N

E

E

G

D

K

K

L

L

I

I

V

V

V

M

V

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

active site: K46 (= K44), S269 (= S266)
binding cysteine: K46 (= K44), T74 (= T71), S75 (= S72), N77 (= N74), T78 (= T75), M101 (= M98), M125 (= M122), M125 (= M122), Q147 (= Q144), F148 (= F145), Q224 (= Q221), G225 (= G222), G225 (= G222), I226 (= I223), A228 (= A225)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
62% identity, 100% coverage: 1:305/306 of query aligns to 73:378/392 of P47999

query
sites
P47999

M

L

R

N

I

I

Y

A

Q

D

D

N

I

A

T

A

E

Q

L

L

I

I

G

G

K

K

T

T

P

P

L

M

L

V

K

Y

L

L

K

N

R

N

V

V

S

V

K

K

D

G

A

C

K

V

A

A

T

S

V

V

V

A

A

A

K

K

L

L

E

E

Y

I

F

M

N

E

P

P

G

C

S

S

V

V

K

K

D

D

R

R

I

I

A

G

Y

Y

N

S

M

M

I

I

K

T

A

D

A

A

E

E

K

K

G

G

L

L

L

I

D

T

K

P

-

G

D

K

T

S

V

V

I

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

A

S

R

K

G

G

Y

Y

R

K

L

L

I

I

I

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

M

V

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

E

G

P

E

A

L

K

G

G

M

M

T

T

G

G

A

A

V

I

N

Q

K

K

A

A

L

E

E

E

L

I

A

L

K

K

E

K

Y

T

P

P

K

N

S

S

F

Y

I

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

E

D

D

T

T

D

R

G

G

K

K

V

I

D

D

I

I

V

L

V

V

G

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

A

E

K

R

K

K

P

P

E

E

V

L

K

K

I

V

I

I

A

G

V

V

E

E

P

P

A

T

A

E

S

S

P

A

V

I

L

L

S

S

G

G

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

K

K

V

N

L

L

N

D

L

L

D

A

V

I

I

V

D

D

E

E

I

Y

I

I

R

A

V

I

E

S

N

S

E

E

D

E

A

A

F

I

A

E

T

T

A

S

K

K

E

Q

L

L

A

A

R

L

E

Q

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E

Q

V

V

A

A

K

K

R

R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

I

V

A

V

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
61% identity, 100% coverage: 1:305/306 of query aligns to 11:316/323 of 4aecA

query
sites
4aecA

M

L

R

N

I

I

Y

A

Q

D

D

N

I

V

T

S

E

Q

L

L

I

I

G

G

K

K

T

T

P

P

L

M

L

V

K

Y

L

L

K

N

R

S

V

I

S

A

K

K

D

G

A

C

K

V

A

A

T

N

V

I

V

A

A

A

K

K

L

L

E

E

Y

I

F

M

N

E

P

P

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

Y

Y

N

S

M

M

I

V

K

T

A

D

A

A

E

E

E

Q

K

K

G

G

L

F

L

I

D

S

K

P

-

G

D

K

T

S

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

A

S

R

R

G

G

Y

Y

R

R

L

L

I

I

I

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

M

E

E

R

R

M

V

L

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

E

A

L

K

G

G

M

M

T

T

G

G

A

A

V

V

N

Q

K

K

A

A

L

E

E

E

L

I

A

L

K

K

E

N

Y

T

P

P

K

D

S

A

F

Y

I

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

D

D

D

T

T

D

K

G

G

K

K

V

V

D

D

I

I

V

F

V

V

G

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

A

E

K

K

K

N

P

P

E

K

V

T

K

Q

I

V

I

I

A

G

V

V

E

E

P

P

A

T

A

E

S

S

P

D

V

I

L

L

S

S

G

G

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

K

V

N

L

L

N

D

L

Q

D

K

V

I

I

M

D

D

E

E

I

V

I

I

R

A

V

I

E

S

N

S

E

E

D

E

A

A

F

I

A

E

T

T

A

A

K

K

E

Q

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E

K

V

V

A

A

K

K

R

R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

I

V

A

V

V

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

active site: K54 (= K44), S277 (= S266)
binding pyridoxal-5'-phosphate: K54 (= K44), N85 (= N74), I188 (≠ V177), G189 (= G178), T190 (= T179), G191 (= G180), G192 (= G181), T193 (= T182), G233 (= G222), S277 (= S266), P304 (= P293)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
62% identity, 99% coverage: 3:305/306 of query aligns to 3:306/309 of 7n2tA

query
sites
7n2tA

I

I

Y

A

Q

K

D

D

I

V

T

T

E

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

R

V

V

S

V

K

D

D

G

A

C

K

V

A

A

T

R

V

V

V

A

A

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

A

G

Y

Y

N

S

M

M

I

I

K

S

A

D

A

A

E

E

E

N

K

K

G

G

L

L

L

I

D

T

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

V

I

A

A

R

K

G

G

Y

Y

R

R

L

L

I

I

T

C

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

M

T

L

I

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

P

D

G

P

E

A

L

R

G

G

M

M

T

K

G

G

A

A

V

V

N

Q

K

K

A

A

L

E

E

E

L

I

A

K

K

A

E

K

Y

T

P

P

K

N

S

S

F

Y

I

I

P

L

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

R

D

G

T

S

D

G

G

G

K

K

V

I

D

D

I

A

V

L

V

V

G

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

A

E

K

Q

K

N

P

P

E

N

V

I

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

G

V

V

L

L

N

D

L

V

D

N

V

L

I

L

D

D

E

E

I

V

I

I

R

Q

V

V

E

S

N

S

E

E

D

E

A

S

F

I

A

E

T

T

A

A

K

K

E

L

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E
x
R

V

I

A

A

K

K

R
|
R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

I

V

V

V

A

V

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

binding phosphate ion: R277 (≠ E276), R281 (= R280)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
59% identity, 100% coverage: 1:305/306 of query aligns to 2:306/310 of 5xoqA

query
sites
5xoqA

M

M

R

P

I

I

Y

F

Q

K

D

D

I

N

T

S

E

E

L

S

I

I

G

G

K

R

T

T

P

P

L

L

L

V

K

Q

L

I

K

N

R

R

V

L

S

T

K

A

D

G

A

L

K

S

A

S

T

R

V

V

V

L

A

A

K

K

L

I

E

E

Y

G

F

R

N

N

P

P

G

A

G

Y

S

S

V

V

K

K

D

C

R

R

I

I

A

G

Y

A

N

A

M

M

I

I

K

W

A

D

A

A

E

E

E

Q

K

S

G

G

L

K

L

L

D

K

K

P

D

G

T

M

V

H

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

F

V

V

A

C

A

A

R

R

G

G

Y

Y

R

K

L

L

I

T

I

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

M

M

L

M

L

L

K

K

A

S

Y

F

G

G

A

A

E

D

I

L

V

V

L

L

T

T

P

P

G

G

E

A

L

D

G

G

M
|
M

T

K

G

G

A

A

V

I

N

S

K

K

A

A

L

E

E

E

L

L

A

A

K

A

E

Q

Y

-

P

P

K

G

S

W

F

F

I

I

P

P

Q

Q

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

P

E

A

I

I

H

H

R

V

Q

K

T

T

A

G

L

P

E

E

I

I

W

W

E

N

D

D

T

T

D

E

G

G

K

Q

V

V

D

D

I

V

V

F

V

V

G

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

A

E

R

V

F

L

L

K

K

A

H

-

E

K

K

P

H

E

P

V

V

K

H

I

V

A

A

V

V

E

E

P

P

A

A

S

S

P

P

V

V

L

L

S

A

G

G

K

P

P

A

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

K

D

V

T

L

F

N

D

L

R

D

S

V

V

I

V

D

D

E

E

I

I

I

L

R

S

V

V

E

T

N

D

E

D

D

E

A

A

F

I

A

E

T

T

A

A

K

R

E

K

L

L

A

A

R

M

E

E

G

G

V

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

L

M

W

A

A

G

A

A

L

L

E

K

V

V

A

A

K

A

R

R

P

P

E

E

N

F

E

A

G

G

K

K

L

T

I

I

V

V

V

T

V

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (= M122), Q144 (= Q144), R218 (≠ P217), H219 (= H218), Q222 (= Q221), G223 (= G222), A226 (= A225)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
58% identity, 99% coverage: 2:305/306 of query aligns to 4:308/322 of P47998

query
sites
P47998

R

R

I

I

Y

A

Q

K

D

D

I

V

T

T

E

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

N

V

V

S

A

K

E

D

G

A

C

K

V

A

G

T

R

V

V

V

A

A

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

G

C

G

S

S

S

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V

K
|
K

D

D

R

R

I

I

A

G

Y

F

N

S

M

M

I

I

K

S

A

D

A

A

E

E

E

K

K

K

G

G

L

L

L

I

D

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

M

F

V

T

A

A

R

K

G

G

Y

Y

R

K

L

L

I

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

M

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

P

D

G

P

E

A

L

K

G

G

M

M

T

K

G

G

A

A

V

I

N

A

K

K

A

A

L

E

E

E

L

I

A

L

K

A

E

K

Y

T

P

P

K

N

S

G

F

Y

I

M

P

L

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H
|
H

R

Y

Q

E

T

T

A
x
G

L

P

E

E

I

I

W

W

E

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

I

G

V

F

V

V

G

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

A

E

K

Q

K

N

P

A

E

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

K
|
K

P

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

S

V

V

L

L

N

N

L

V

D

D

V

L

I

I

D

D

E

E

I

V

R

Q

V

V

E

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

E

Q

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E

K

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

F

V

V

V

A

V

I

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

K46 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T71) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S72) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N74) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T75) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q144) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H154) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A159) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
T182 (= T179) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T182) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (= K214) mutation to A: Impaired interaction with SAT1.
H221 (= H218) mutation to A: Impaired interaction with SAT1.
K222 (= K219) mutation to A: Impaired interaction with SAT1.
S269 (= S266) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
58% identity, 99% coverage: 2:305/306 of query aligns to 2:306/320 of 2isqA

query
sites
2isqA

R

R

I

I

Y

A

Q

K

D

D

I

V

T

T

E

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

N

V

V

S

A

K

E

D

G

A

C

K

V

A

G

T

R

V

V

V

A

A

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

Y

F

N

S

M

M

I

I

K

S

A

D

A

A

E

E

E

K

K

K

G

G

L

L

L

I

D

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

M

F

V

T

A

A

R

K

G

G

Y

Y

R

K

L

L

I

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

M

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

P

D

G

P

E

A

L

K

G
|
G

M
|
M

T
x
K

G

G

A

A

V

I

N

A

K

K

A

A

L

E

E

E

L

I

A

L

K

A

E

K

Y

T

P

P

K

N

S

G

F

Y

I

M

P

L

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

I

G

V

F

V

V

G

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

A

E

K

Q

K

N

P

A

E

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

K

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

S

V

V

L

L

N

N

L

V

D

D

V

L

I

I

D

D

E

E

I

V

R

Q

V

V

E

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

E

Q

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E

K

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

F

V

V

V

A

V

I

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

active site: K44 (= K44), S267 (= S266)
binding pyridoxal-5'-phosphate: K44 (= K44), N75 (= N74), G177 (= G176), G179 (= G178), T180 (= T179), G181 (= G180), T183 (= T182), G223 (= G222), S267 (= S266), P294 (= P293)
binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), G122 (= G121), M123 (= M122), K124 (≠ T123), G217 (= G216), P218 (= P217), H219 (= H218), Q222 (= Q221), G223 (= G222)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
58% identity, 99% coverage: 2:305/306 of query aligns to 2:306/320 of 1z7yA

query
sites
1z7yA

R

R

I

I

Y

A

Q

K

D

D

I

V

T

T

E

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

N

V

V

S

A

K

E

D

G

A

C

K

V

A

G

T

R

V

V

V

A

A

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

A

G

Y

F

N

S

M

M

I

I

K

S

A

D

A

A

E

E

E

K

K

K

G

G

L

L

L

I

D

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

M

F

V

T

A

A

R

K

G

G

Y

Y

R

K

L

L

I

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

M

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

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V

L

L

T

T

P

D

G

P

E

A

L

K

G

G

M

M

T

K

G

G

A

A

V

I

N

A

K

K

A

A

L

E

E

E

L

I

A

L

K

A

E

K

Y

T

P

P

K

N

S

G

F

Y

I

M

P

L

Q

Q

Q
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Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

I

G

V

F

V

V

G

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

A

E

K

Q

K

N

P

A

E

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

A

V

A

E

S

S

P

A

V

I

L

L

S

S

G

G

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

K

S

V

V

L

L

N

N

L

V

D

D

V

L

I

I

D

D

E

E

I

V

R

Q

V

V

E

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

E

Q

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

L

A

W

A

A

A

L

I

E

K

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

E

A

G

G

K

K

L

L

I

F

V

V

V

A

V

I

L

F

P
|
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

active site: A44 (≠ K44), S267 (= S266)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G73), N75 (= N74), T76 (= T75), Q145 (= Q144), I178 (≠ V177), G179 (= G178), T180 (= T179), G181 (= G180), T183 (= T182), G223 (= G222), S267 (= S266), P294 (= P293), S295 (≠ D294)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
53% identity, 99% coverage: 2:305/306 of query aligns to 10:314/329 of 3vbeC

query
sites
3vbeC

R

N

I

I

Y

K

Q

K

D

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T

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E

Q

L

L

I

I

G

G

K

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T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

K

V

V

S

T

K

E

D

G

A

C

K

G

A

A

T

Y

V

V

V

A

A

V

K

K

L

Q

E

E

Y

M

F

M

N

Q

P

P

G

T

G

A

S

S

V

I

K
|
K

D

D

R

R

I

P

A

A

Y

Y

N

A

M

M

I

I

K

T

A

D

A

A

E

E

K

K

G

N

L

L

L

I

D

T

K

P

-

G

D

K

T

T

V

T

I

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

G

S

L

M

A

A

M

F

V

M

A

A

A

M

R

K

G

G

Y

Y

R

K

L

M

I

V

I

L

T

T

M

M

P

P

E

S

T

Y

M

T

S

S

V

L

E

E

R

R

M

V

L

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

I

L

L

T

T

P

D

G

P

E

A

L

K

G

G

M

M

T

G

G

G

A

T

V

V

N

K

K

K

A

A

L

Y

E

E

L

L

A

L

K

E

E

N

Y

T

P

P

K

N

S

A

F

H

I

M

P

L

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

T

E

Q

I

V

H

H

R

F

Q

E

T

T

A

G

L

P

E

E

I

I

W

W

E

E

D

D

T

T

D

N

G

G

K

Q

V

V

D

D

I

I

V

F

V

V

G
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

I

V

T

S

G

G

V

V

G

G

E

Q

V

Y

L

L

K

K

A

S

K

K

K

N

P

P

E

N

V

V

K

K

I

I

I

Y

A

G

V

V

E
|
E

P

P

A

S

A

E

S
|
S

P

N

V

V

L

L

S

N

G

G

K

K

P

P

G

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

I

K

P

P

K

D

V

I

L

L

N

D

L

L

D

D

V

V

I

M

D

E

E

K

I

V

I

L

R

E

V

V

E

S

N

S

E

E

D

D

A

A

F

V

A

N

T

M

A

A

K

R

E

V

L

L

A

A

R

L

E

K

E

E

G

G

V

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

A

N

L

T

W

V

A

A

L

L

E

R

V

L

A

A

K

Q

R

L

P

P

E

E

N

N

E

K

G

G

K

K

L

L

I

I

V

V

V

T

V

V

L

H

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

P

V

L

L

F

F

active site: K52 (= K44), S81 (= S72), E212 (= E203), S216 (= S207), S275 (= S266), P302 (= P293)
binding pyridoxal-5'-phosphate: K52 (= K44), N83 (= N74), M184 (≠ G175), G187 (= G178), S188 (≠ T179), G189 (= G180), T191 (= T182), G231 (= G222), S275 (= S266), P302 (= P293)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
52% identity, 99% coverage: 2:305/306 of query aligns to 3:307/322 of 3vc3A

query
sites
3vc3A

R

N

I

I

Y

K

Q

K

D

H

I

V

T

S

E

Q

L

L

I

I

G

G

K

R

T

T

P

P

L

L

L

V

K

Y

L

L

K

N

R

K

V

V

S

T

K

E

D

G

A

C

K

G

A

A

T

Y

V

V

V

A

A

V

K

K

L

Q

E

E

Y

M

F

M

N

Q

P

P

G

T

G

A

S

S

V

I

K
x
A

D

D

R

R

I

P

A

A

Y

Y

N

A

M

M

I

I

K

T

A

D

A

A

E

E

K

K

G

N

L

L

L

I

D

T

K

P

-

G

D

K

T

T

V

T

I

L

I

I

E

E

P

P

T
|
T