SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_075859776.1 NCBI__GCF_001950255.1:WP_075859776.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

1dbfA Chorismate mutase from bacillus subtilis at 1.30 angstrom (see paper)
45% identity, 98% coverage: 3:118/118 of query aligns to 2:119/127 of 1dbfA

query
sites
1dbfA
V
 
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T
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x
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E
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E
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Sites not aligning to the query:

P19080 Chorismate mutase AroH; CM; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see 2 papers)
44% identity, 98% coverage: 3:118/118 of query aligns to 2:119/127 of P19080

query
sites
P19080
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E
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E
A
 
E
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L
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Q
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T
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K
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Q
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L
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L
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E
E
 
K
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I
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E
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E
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N
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K
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E
 
E
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D
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V
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L

3zp7A Arg90cit chorismate mutase of bacillus subtilis in complex with chorismate and prephenate (see paper)
44% identity, 97% coverage: 3:116/118 of query aligns to 2:117/117 of 3zp7A

query
sites
3zp7A
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E
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E
A
 
E
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I
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L
E
 
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T
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Q
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L
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L
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E
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K
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I
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E
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E
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N
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H
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V
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2chtA Crystal structures of the monofunctional chorismate mutase from bacillus subtilis and its complex with a transition state analog (see paper)
43% identity, 95% coverage: 3:114/118 of query aligns to 1:114/114 of 2chtA

query
sites
2chtA
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E
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E
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E
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Q
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E
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1comA The monofunctional chorismate mutase from bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications on the mechanism of enzymatic reaction (see paper)
43% identity, 95% coverage: 3:114/118 of query aligns to 1:114/114 of 1comA

query
sites
1comA
V
 
M
V
 
I
K
 
R
G
 
G
I
 
I
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G
 
G
A
 
A
I
 
T
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T
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V
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E
E
 
R
N
 
D
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T
A
 
E
E
 
E
A
 
E
I
 
I
E
 
L
E
 
Q
Q
 
K
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T
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K
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Q
L
 
L
L
 
L
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E
E
 
K
I
 
I
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I
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E
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E
N
 
N
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H
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T
K
 
K
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P
E
 
E
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D
I
 
V
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V
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Q
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M
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L
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A
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P
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H
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A
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P
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K
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A
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L
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E
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K
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A
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V
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V
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L

Query Sequence

>WP_075859776.1 NCBI__GCF_001950255.1:WP_075859776.1
MEVVKGIRGAITVTENSAEAIEEQTKKLLTEIFLQNNLKKEKIISIFFTLTPDLNAQFPA
TAARKAGLTEVPLLCAQEIAVPGSLKKVIRILILTYLPENQKVHHLYLEGAKVLRPDF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory