SitesBLAST
Comparing WP_076478048.1 NCBI__GCF_900156495.1:WP_076478048.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6jixA The cyrstal structure of taurine:2-oxoglutarate aminotransferase from bifidobacterium kashiwanohense, in complex with plp and glutamate (see paper)
42% identity, 94% coverage: 29:460/462 of query aligns to 12:445/447 of 6jixA
- active site: W21 (= W38), Y141 (= Y158), D250 (= D264), K279 (= K293)
- binding glutamic acid: S20 (= S37), W21 (= W38), P82 (= P100), A83 (≠ Q101), A83 (≠ Q101), Y84 (= Y102), L310 (= L324)
- binding pyridoxal-5'-phosphate: G114 (= G131), A115 (= A132), Y141 (= Y158), H142 (= H159), E217 (= E231), D250 (= D264), V252 (= V266), M253 (= M267), K279 (= K293), L310 (= L324), T311 (= T325)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
37% identity, 89% coverage: 46:454/462 of query aligns to 31:444/448 of 6io1B
- active site: Y151 (= Y158), D257 (= D264), K286 (= K293)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G130), G118 (= G131), A119 (= A132), N122 (= N135), Y151 (= Y158), H152 (= H159), D257 (= D264), V259 (= V266), I260 (≠ M267), K286 (= K293)
Sites not aligning to the query:
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
33% identity, 92% coverage: 28:453/462 of query aligns to 5:435/443 of 6fyqA
- active site: F15 (≠ W38), Y147 (= Y158), D243 (= D264), K272 (= K293)
- binding pyridoxal-5'-phosphate: G114 (= G131), S115 (≠ A132), Y147 (= Y158), H148 (= H159), G149 (= G160), E210 (= E231), D243 (= D264), V245 (= V266), I246 (≠ M267), K272 (= K293)
5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
36% identity, 87% coverage: 54:453/462 of query aligns to 35:445/449 of 5lh9D
- active site: Y148 (= Y158), D255 (= D264), K284 (= K293), T321 (= T325)
- binding pyridoxal-5'-phosphate: G115 (= G131), S116 (≠ A132), Y148 (= Y158), H149 (= H159), G150 (= G160), E222 (= E231), D255 (= D264), V257 (= V266), K284 (= K293)
5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
36% identity, 87% coverage: 54:453/462 of query aligns to 33:443/447 of 5lhaA
- active site: Y146 (= Y158), D253 (= D264), K282 (= K293), T319 (= T325)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G131), S114 (≠ A132), Y146 (= Y158), H147 (= H159), G148 (= G160), E220 (= E231), D253 (= D264), K282 (= K293), Y318 (≠ L324), T319 (= T325)
6s54A Transaminase from pseudomonas fluorescens (see paper)
33% identity, 91% coverage: 33:454/462 of query aligns to 18:449/453 of 6s54A
- active site: M20 (≠ F35), Y152 (= Y158), D258 (= D264), K287 (= K293)
- binding pyridoxal-5'-phosphate: G119 (= G131), S120 (≠ A132), Y152 (= Y158), H153 (= H159), G154 (= G160), E225 (= E231), D258 (= D264), V260 (= V266), I261 (≠ M267), K287 (= K293)
3fcrA Crystal structure of putative aminotransferase (yp_614685.1) from silicibacter sp. Tm1040 at 1.80 a resolution
32% identity, 90% coverage: 30:445/462 of query aligns to 12:448/458 of 3fcrA
- active site: S20 (vs. gap), Y153 (= Y158), E227 (= E231), D260 (= D264), V263 (≠ M267), K289 (= K293), T326 (= T325), G430 (≠ P422)
- binding pyridoxal-5'-phosphate: G120 (= G131), S121 (≠ A132), Y153 (= Y158), H154 (= H159), E227 (= E231), D260 (= D264), V262 (= V266), K289 (= K293)
Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 90% coverage: 38:453/462 of query aligns to 63:488/504 of Q94CE5
- C98 (≠ N74) mutation to Y: In her1-1; loss of E2-hexenal responses.
- G103 (≠ H79) mutation to N: In her1-2; loss of E2-hexenal responses.
- G271 (≠ A237) mutation to E: In pop2-6; Loss of activity and complete sterility.
- G312 (≠ S278) mutation to D: In pop2-7; loss of activity.
D6R3B6 Vanillin aminotransferase; Putative aminotransferase; pAMT; EC 2.6.1.119 from Capsicum frutescens (Cayenne pepper) (Tabasco pepper) (see paper)
30% identity, 89% coverage: 41:453/462 of query aligns to 22:444/459 of D6R3B6
- SAGL 155:158 (≠ AINL 165:168) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.
3gjuA Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 a resolution
34% identity, 89% coverage: 30:441/462 of query aligns to 13:441/458 of 3gjuA
- active site: S21 (≠ W38), Y154 (= Y158), E228 (= E231), D261 (= D264), V264 (≠ M267), K290 (= K293), T327 (= T325), G430 (≠ P422)
- binding pyridoxal-5'-phosphate: S120 (≠ G130), G121 (= G131), S122 (≠ A132), Y154 (= Y158), H155 (= H159), E228 (= E231), D261 (= D264), V263 (= V266), K290 (= K293)
5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
33% identity, 93% coverage: 28:458/462 of query aligns to 11:455/460 of 5kr6B