SitesBLAST
Comparing WP_076578503.1 NCBI__GCF_001971705.1:WP_076578503.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
I3VE77 2-hydroxyisobutanoyl-CoA mutase large subunit; 2-hydroxyisobutyryl-CoA mutase large subunit; HCM large subunit; EC 5.4.99.64 from Aquincola tertiaricarbonis (see 2 papers)
52% identity, 96% coverage: 17:561/566 of query aligns to 19:558/562 of I3VE77
- YPTM 76:79 (= YPTM 75:78) binding (3S)-3-hydroxybutanoyl-CoA
- TMR 86:88 (= TMR 85:87) binding (3S)-3-hydroxybutanoyl-CoA
- I90 (≠ F89) mutation to A: 6-fold decrease in catalytic efficiency with 2-hydroxyisobutyryl-CoA as substrate. 320-fold decrease in catalytic efficiency with (S)-3-hydroxybuytryl-CoA as substrate. 6-fold increase in catalytic efficiency with (R)-3-hydroxybutyryl-CoA as substrate. No change in catalytic efficiencies with pivalyl-CoA and isovaleryl-CoA as substrates.; mutation I->F,Y: Loss of activity.; mutation to L: 37-fold decrease in catalytic efficiency with 2-hydroxyisobutyryl-CoA as substrate. 290-fold decrease in catalytic efficiency with (S)-3-hydroxybuytryl-CoA as substrate. Does not show any significant activities with pivalyl-CoA and isovaleryl-CoA.; mutation to V: 100-fold decrease in catalytic efficiency with (S)-3-hydroxybuytryl-CoA as substrate. No change in catalytic efficiencies with pivalyl-CoA and isovaleryl-CoA as substrates.
- D117 (≠ A116) binding (3S)-3-hydroxybutanoyl-CoA; mutation to A: 2-fold increase in catalytic efficiency with 2-hydroxyisobutyryl-CoA as substrate. Small increase in catalytic efficiency with (S)-3-hydroxybuytryl-CoA as substrate. 1800-fold increase in catalytic efficiency with (R)-3-hydroxybutyryl-CoA as substrate.; mutation to V: 1.5-fold increase in catalytic efficiency with 2-hydroxyisobutyryl-CoA as substrate. 3-fold decrease in catalytic efficiency with (S)-3-hydroxybuytryl-CoA as substrate. 1300-fold increase in catalytic efficiency with (R)-3-hydroxybutyryl-CoA as substrate. 74-fold increase in catalytic efficiency with pivalyl-CoA as substrate.
- TVQ 196:198 (≠ TLQ 195:197) binding (3S)-3-hydroxybutanoyl-CoA
- R235 (≠ Q234) binding (3S)-3-hydroxybutanoyl-CoA
- N240 (≠ S239) binding (3S)-3-hydroxybutanoyl-CoA
- H245 (= H244) binding (3S)-3-hydroxybutanoyl-CoA
- R284 (= R283) binding (3S)-3-hydroxybutanoyl-CoA
4r3uA Crystal structure of 2-hydroxyisobutyryl-coa mutase (see paper)
52% identity, 96% coverage: 17:561/566 of query aligns to 18:557/557 of 4r3uA
- active site: I89 (≠ F89), Y243 (= Y243), H244 (= H244)
- binding 3-hydroxybutanoyl-coenzyme a: Y75 (= Y75), T77 (= T77), M78 (= M78), R82 (= R82), T85 (= T85), R87 (= R87), I89 (≠ F89), D116 (≠ A116), S164 (= S164), T166 (= T166), T195 (= T195), Q197 (= Q197), R234 (≠ Q234), N236 (≠ H236), N239 (≠ S239), Y243 (= Y243), H244 (= H244), R283 (= R283), F287 (= F287), R327 (≠ K327), F328 (= F328), H329 (= H329), Q331 (= Q331), Q362 (= Q362)
- binding S-{(3R,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} 2-hydroxy-2-methylpropanethioate: Y75 (= Y75), T77 (= T77), M78 (= M78), R82 (= R82), T85 (= T85), R87 (= R87), I89 (≠ F89), D116 (≠ A116), S164 (= S164), T166 (= T166), T195 (= T195), Q197 (= Q197), R234 (≠ Q234), N236 (≠ H236), N239 (≠ S239), H244 (= H244), R283 (= R283), F287 (= F287), R327 (≠ K327), F328 (= F328), H329 (= H329), Q331 (= Q331), Q362 (= Q362)
- binding cobalamin: D116 (≠ A116), M119 (= M119), E139 (= E139), Q207 (= Q207), E209 (= E209), E247 (= E247), A334 (≠ G334), E371 (= E371), A372 (= A372), A374 (= A374)
P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
44% identity, 95% coverage: 30:564/566 of query aligns to 53:592/750 of P22033
- I69 (≠ V44) to V: in MMAM; likely benign; dbSNP:rs115923556
- P86 (= P65) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
- G87 (= G66) to E: in MMAM; mut0; dbSNP:rs1554160986
- R93 (= R72) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
- G94 (= G73) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
- P95 (= P74) to R: in MMAM; mut0; dbSNP:rs190834116
- YPTM 96:99 (= YPTM 75:78) binding malonyl-CoA
- Y100 (= Y79) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
- W105 (= W84) to R: in MMAM; mut0; dbSNP:rs121918249
- TIRQY 106:110 (≠ TMRQF 85:89) binding malonyl-CoA
- R108 (= R87) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; to H: in MMAM; mut0; dbSNP:rs483352778
- Q109 (= Q88) to R: in MMAM; mut0; dbSNP:rs1461110052
- G133 (= G112) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
- A137 (= A116) to V: in MMAM; mut0; dbSNP:rs941483851
- D139 (= D118) to N: in MMAM; uncertain significance; dbSNP:rs879253829
- L140 (≠ M119) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- A141 (≠ P120) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
- H143 (≠ L122) to Y: in MMAM; mut0
- G145 (= G124) to S: in MMAM; mut0
- S148 (= S127) to L: in MMAM; mut0; dbSNP:rs1300547552
- D156 (≠ E135) to N: in MMAM; mut-
- G158 (= G137) to V: in MMAM; mut0
- G161 (= G140) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; to V: in MMAM; decreased protein expression
- F174 (= F153) to S: in MMAM; mut0; dbSNP:rs864309733
- M186 (≠ F165) to V: in MMAM; mut-; dbSNP:rs148331800
- T187 (= T166) to S: in MMAM; mut0; dbSNP:rs879253830
- N189 (= N168) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
- A191 (≠ S170) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
- A197 (= A176) to E: in MMAM; mut0
- G203 (≠ A182) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
- E205 (≠ Q184) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
- G215 (= G194) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
- TIQ 216:218 (≠ TLQ 195:197) binding malonyl-CoA
- Q218 (= Q197) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
- N219 (= N198) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
- R228 (≠ Q207) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
- T230 (≠ E209) to I: in MMAM; mut-; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
- Y231 (≠ W210) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
- K255 (≠ Q234) binding malonyl-CoA
- S262 (= S241) to N: in MMAM; mut0
- H265 (= H244) binding malonyl-CoA; to Y: in MMAM; mut-
- E276 (= E255) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
- L281 (= L260) to S: in MMAM; mut0; dbSNP:rs796052007
- G284 (= G263) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
- S288 (≠ V267) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
- G291 (≠ A270) to E: in MMAM; mut0
- Q293 (≠ E272) to P: in MMAM; mut0
- RLS 304:306 (= RLS 283:285) binding malonyl-CoA
- L305 (= L284) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
- S306 (= S285) to F: in MMAM; mut0; dbSNP:rs1085307929
- W309 (≠ F288) to G: in MMAM; decreased protein expression
- G312 (≠ H291) to V: in MMAM; mut0; dbSNP:rs864309734
- Y316 (≠ F295) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
- A324 (= A303) to T: in MMAM; mut-; dbSNP:rs780387525
- R326 (= R305) to K: in MMAM; uncertain significance; dbSNP:rs758577372
- L328 (≠ I307) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
- S344 (= S323) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
- L346 (≠ R325) natural variant: Missing (in MMAM; mut0)
- L347 (= L326) to R: in MMAM; mut0; dbSNP:rs1026703654
- H350 (= H329) to Y: in MMAM; mut0; dbSNP:rs1407914109
- L358 (= L337) to P: in MMAM; mut0
- N366 (= N345) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
- R369 (= R348) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
- T370 (≠ V349) to P: in MMAM; mut0; dbSNP:rs368790885
- A377 (≠ G356) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
- Q383 (= Q362) to H: in MMAM; mut0; to P: in MMAM; mut0
- H386 (= H365) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
- T387 (= T366) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability
- N388 (= N367) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
- S389 (= S368) natural variant: Missing (in MMAM; mut0)
- I412 (= I391) natural variant: Missing (in MMAM; mut0)
- P424 (= P403) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
- G426 (= G405) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
- G427 (= G406) to D: in MMAM; mut0; dbSNP:rs753288303
- G454 (= G439) to E: in MMAM; mut0
- A499 (≠ S484) to T: in dbSNP:rs2229385
- I505 (= I490) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- Q514 (= Q498) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression
- L518 (= L502) to P: in MMAM; mut0; dbSNP:rs864309738
- R532 (≠ D516) to H: in dbSNP:rs1141321
- A535 (≠ E519) to P: in MMAM; mut0; dbSNP:rs760183775
- A552 (≠ L535) to V: in MMAM; uncertain significance; dbSNP:rs879253845
- C560 (≠ A543) to Y: in MMAM; mut0; dbSNP:rs1238333040
- T566 (≠ M549) to R: in MMAM; mut0
- F573 (vs. gap) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
- Y587 (= Y559) to C: in MMAM; mut-
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 7:750 natural variant: Missing (in MMAM; mut-)
- 50 binding malonyl-CoA
- 152:750 natural variant: Missing (in MMAM; mut0)
- 228:750 natural variant: Missing (in MMAM; mut0)
- 597 I → R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
- 615 P → L: in MMAM; mut0; affects proper folding; reduced strongly protein level; P → R: in MMAM; mut0; dbSNP:rs1554158777; P → T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
- 616 R → C: in MMAM; mut0; dbSNP:rs765284825
- 617 L → R: in MMAM; mut0; dbSNP:rs1554158775
- 621 K → N: in MMAM; mut0
- 623 G → R: in MMAM; mut0; dbSNP:rs121918254
- 624 Q → R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
- 625 D → G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; D → V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
- 626 G → C: in MMAM; mut-; dbSNP:rs982110849
- 627 binding axial binding residue; H → R: in MMAM; mut0; dbSNP:rs372486357
- 630 G → E: in MMAM; mut0; dbSNP:rs143023066
- 633 V → G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
- 637 G → E: in MMAM; mut-; G → R: in MMAM; mut0; dbSNP:rs781501004
- 638 F → I: in MMAM; mut0
- 640 D → Y: in MMAM; mut0; dbSNP:rs865815395
- 642 G → R: in MMAM; mut-; dbSNP:rs747897332
- 648 G → D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
- 669 V → E: in MMAM; mut0; dbSNP:rs1360470463
- 671 I → V: in dbSNP:rs8589
- 674 L → F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
- 678 H → R: in MMAM; mut-; dbSNP:rs147094927
- 684 natural variant: E -> EL (in MMAM; mut-)
- 685 L → R: in MMAM; mut-; dbSNP:rs864309739
- 694 R → L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; R → W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
- 700 M → K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
- 703 G → R: in MMAM; mut0; dbSNP:rs121918255
- 717 G → V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
- 723 G → D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681
8gjuJ Crystal structure of human methylmalonyl-coa mutase (mmut) in complex with methylmalonic acidemia type a protein (mmaa), coenzyme a, and gdp (see paper)
44% identity, 94% coverage: 30:561/566 of query aligns to 18:543/689 of 8gjuJ
- binding coenzyme a: Y61 (= Y75), T63 (= T77), R68 (= R82), T71 (= T85), R73 (= R87), S150 (= S164), T152 (= T166), T181 (= T195), Q183 (= Q197), N222 (≠ H236), R269 (= R283), S271 (= S285), R313 (≠ K327), A314 (≠ F328), H315 (= H329), Q348 (= Q362)
2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
44% identity, 95% coverage: 30:564/566 of query aligns to 18:557/714 of 2xiqA
- active site: Y75 (≠ F89), Y229 (= Y243), H230 (= H244)
- binding cobalamin: Y75 (≠ F89), L105 (≠ M119), H108 (≠ L122), A125 (≠ E139), R193 (≠ Q207), E233 (= E247), G320 (= G334), W321 (≠ Q335), E357 (= E371), G360 (≠ A374), L361 (= L375)
- binding malonyl-coenzyme a: Y61 (= Y75), T63 (= T77), M64 (= M78), R68 (= R82), T71 (= T85), R73 (= R87), Y75 (≠ F89), S150 (= S164), T152 (= T166), T181 (= T195), R193 (≠ Q207), K220 (≠ Q234), H230 (= H244), R269 (= R283), S271 (= S285), F273 (= F287), R313 (≠ K327), A314 (≠ F328), H315 (= H329), Q317 (= Q331), Q348 (= Q362)
Sites not aligning to the query:
- active site: 586, 590, 592
- binding cobalamin: 591, 592, 593, 594, 595, 599, 635, 637, 639, 641, 667, 668, 687, 688, 691
8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
44% identity, 95% coverage: 30:564/566 of query aligns to 17:556/708 of 8dyjB