SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_076581962.1 NCBI__GCF_001971705.1:WP_076581962.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
36% identity, 99% coverage: 2:127/127 of query aligns to 4:132/138 of 3oa4A

query
sites
3oa4A
H
 
K
F
 
L
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
V
E
 
T
D
 
S
A
 
I
Q
 
K
E
 
D
L
 
V
A
 
L
E
 
P
L
 
F
Y
 
Y
S
 
V
D
 
G
L
 
S
F
 
L
G
 
K
L
 
L
A
 
K
V
 
L
A
 
L
H
 
G
E
 
M
E
 
E
E
 
D
F
 
L
-
 
P
-
 
S
D
 
Q
G
 
G
M
 
V
R
 
K
V
x
I
V
 
A
F
 
F
L
 
L
E
 
E
C
 
I
G
 
G
D
 
E
G
 
S
Y
 
K
F
 
I
E
|
E
L
 
L
L
 
L
E
 
E
P
 
P
F
 
L
-
 
S
E
 
E
D
 
E
G
 
S
T
 
P
I
 
I
A
 
A
Q
 
K
Y
 
F
L
 
I
E
 
Q
A
 
K
N
 
R
G
 
G
S
 
E
G
 
G
I
 
I
H
 
H
H
|
H
L
 
I
A
 
A
L
 
I
A
 
G
T
 
V
E
 
K
D
 
S
I
 
I
E
 
E
A
 
E
A
 
R
L
 
I
E
 
Q
T
 
E
A
 
V
R
 
K
E
 
E
H
 
N
D
 
G
V
 
V
S
 
Q
L
 
M
I
 
I
D
 
N
E
 
D
E
 
E
P
 
P
R
 
V
P
 
P
G
 
G
A
 
A
W
 
R
G
 
G
H
 
A
S
 
Q
V
 
V
A
 
A
F
 
F
L
 
L
H
 
H
P
 
P
K
 
R
D
 
S
T
 
A
G
 
R
G
 
G
I
 
V
L
 
L
L
 
Y
E
|
E
L
 
F
V
 
C
E
 
E
H
 
K

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/141 of 6wf6A

query
sites
6wf6A
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
 
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
 
A
I
 
V
A
 
G
Q
 
K
Y
 
W
L
 
L
E
 
A
A
 
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
 
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
 
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
 
G
A
 
S
W
 
M
G
 
G
H
 
S
S
 
R
V
 
I
A
 
T
F
 
F
L
 
L
H
 
H
P
 
P
K
 
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
 
L
L
 
T
E
|
E
L
 
L
V
 
V

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/144 of 6xbqA

query
sites
6xbqA
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
x
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
x
A
I
 
V
A
 
G
Q
x
K
Y
x
W
L
 
L
E
 
A
A
 
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
|
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
x
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
 
G
A
 
S
W
 
M
G
 
G
H
 
S
S
 
R
V
 
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
|
L
L
 
T
E
|
E
L
 
L
V
 
V

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/144 of 6wfiA

query
sites
6wfiA
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
x
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
x
A
I
 
V
A
 
G
Q
x
K
Y
x
W
L
 
L
E
 
A
A
 
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
|
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
x
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
|
G
A
x
S
W
 
M
G
 
G
H
 
S
S
 
R
V
x
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
 
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
|
L
L
 
T
E
|
E
L
 
L
V
 
V

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/144 of 6wf7A

query
sites
6wf7A
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
x
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
x
A
I
 
V
A
 
G
Q
 
K
Y
x
W
L
 
L
E
 
A
A
 
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
|
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
x
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
|
G
A
x
S
W
 
M
G
 
G
H
 
S
S
 
R
V
x
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
|
L
L
 
T
E
|
E
L
 
L
V
 
V

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/139 of 6wfhA

query
sites
6wfhA
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
x
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
x
A
I
 
V
A
 
G
Q
x
K
Y
x
W
L
 
L
E
 
A
A
 
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
|
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
x
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
|
G
A
x
S
W
 
M
G
 
G
H
 
S
S
 
R
V
 
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
|
L
L
 
T
E
|
E
L
 
L
V
 
V

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
38% identity, 98% coverage: 2:125/127 of query aligns to 4:136/140 of 6xbtA

query
sites
6xbtA
H
 
R
F
 
I
E
 
D
H
|
H
A
 
I
G
 
G
I
 
I
A
 
A
T
 
C
E
 
H
D
 
D
A
 
L
Q
 
D
E
 
A
L
 
T
A
 
V
E
 
E
L
 
F
Y
 
Y
S
 
R
D
 
A
L
 
T
F
 
Y
G
 
G
L
 
F
A
 
E
V
 
V
A
 
F
H
 
H
E
 
T
E
 
E
-
 
V
-
 
N
E
 
E
F
 
E
D
x
Q
G
 
G
M
 
V
R
 
R
V
x
E
V
 
A
F
 
M
L
 
L
E
 
K
C
 
I
G
 
N
D
 
D
-
 
T
-
 
S
-
 
D
-
 
G
-
 
G
-
 
A
G
 
S
Y
 
Y
F
 
L
E
x
A
L
 
L
L
 
L
E
 
E
P
 
P
F
 
T
-
 
R
E
 
E
D
 
D
G
 
S
T
x
A
I
 
V
A
 
G
Q
x
K
Y
x
W
L
 
L
E
 
A
A
x
K
N
 
N
G
 
G
S
 
E
G
 
G
I
 
V
H
|
H
H
|
H
L
 
I
A
 
A
L
 
F
A
 
G
T
 
T
E
 
A
D
 
D
I
 
V
E
 
D
A
 
A
A
 
D
L
 
A
E
 
A
T
 
D
A
 
I
R
 
R
E
 
D
H
 
K
D
 
G
V
 
V
S
 
R
L
 
V
I
x
L
D
 
Y
E
 
D
E
 
E
P
 
P
R
 
R
P
 
R
G
|
G
A
x
S
W
 
M
G
 
G
H
 
S
S
 
R
V
 
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
D
 
D
T
 
C
G
 
H
G
 
G
I
 
V
L
|
L
L
 
T
E
|
E
L
 
L
V
 
V

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
35% identity, 97% coverage: 2:124/127 of query aligns to 47:173/176 of Q96PE7

query
sites
Q96PE7
H
 
R
F
 
L
E
 
N
H
|
H
A
 
V
G
 
A
I
 
I
A
 
A
T
 
V
E
 
P
D
 
D
A
 
L
Q
 
E
E
 
K
L
 
A
A
 
A
E
 
A
L
 
F
Y
 
Y
S
 
K
D
 
N
L
 
I
F
 
L
G
 
G
L
 
A
A
 
Q
V
 
V
A
 
S
H
 
E
E
 
A
E
 
V
E
 
P
F
 
L
D
 
P
-
 
E
-
 
H
G
 
G
M
 
V
R
 
S
V
 
V
V
 
V
F
 
F
L
 
V
E
 
N
C
 
L
G
 
G
D
 
N
G
 
T
Y
 
K
F
 
M
E
 
E
L
 
L
L
 
L
E
 
H
P
 
P
F
 
L
-
 
G
E
x
R
D
 
D
G
 
S
T
 
P
I
 
I
A
 
A
Q
 
G
Y
 
F
L
 
L
E
 
Q
A
 
K
N
 
N
G
 
K
S
 
A
G
 
G
-
 
G
I
 
M
H
 
H
H
|
H
L
 
I
A
 
C
L
 
I
A
 
E
T
 
V
E
 
D
D
 
N
I
 
I
E
 
N
A
 
A
A
 
A
L
 
V
E
 
M
T
 
D
A
 
L
R
 
K
E
 
K
H
 
K
D
 
K
V
 
I
S
 
R
L
 
S
I
 
L
D
 
S
E
 
E
E
 
E
P
 
V
R
 
K
P
 
I
G
 
G
A
 
A
W
 
H
G
 
G
H
 
K
S
 
P
V
 
V
A
 
I
F
 
F
L
 
L
H
 
H
P
 
P
K
 
K
D
 
D
T
 
C
G
 
G
G
 
G
I
 
V
L
 
L
L
 
V
E
|
E
L
 
L

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
34% identity, 97% coverage: 2:124/127 of query aligns to 11:135/138 of 6qh4C

query
sites
6qh4C
H
 
R
F
 
L
E
 
N
H
|
H
A
 
V
G
 
A
I
 
I
A
 
A
T
 
V
E
 
P
D
 
D
A
 
L
Q
 
E
E
 
K
L
 
A
A
 
A
E
 
A
L
 
F
Y
 
Y
S
 
K
D
 
N
L
 
I
F
 
L
G
 
G
L
 
A
A
 
Q
V
 
V
A
 
S
H
 
E
E
 
A
E
 
V
E
 
P
F
 
L
D
 
P
-
 
E
-
 
H
G
 
G
M
 
V
R
 
S
V
|
V
V
 
V
F
 
F
L
 
V
E
 
N
C
 
L
G
 
G
D
 
N
G
 
T
Y
 
K
F
 
M
E
|
E
L
 
L
L
 
L
E
 
H
P
 
P
F
 
L
E
 
G
-
 
L
D
 
D
G
 
S
T
 
P
I
 
I
A
 
A
Q
 
G
Y
 
F
L
 
L
E
 
Q
A
 
K
N
 
N
G
 
K
S
 
A
G
 
G
-
 
G
I
 
M
H
 
H
H
|
H
L
 
I
A
 
C
L
 
I
A
 
E
T
 
V
E
 
D
D
 
N
I
 
I
E
 
N
A
 
A
A
 
A
L
 
V
E
 
M
T
 
D
A
 
L
R
 
K
E
 
K
H
 
K
D
 
K
V
 
I
S
 
C
L
 
S
I
 
L
D
 
S
E
 
V
E
 
-
P
 
-
R
 
K
P
 
I
G
 
G
A
 
A
W
 
H
G
 
G
H
 
K
S
 
P
V
 
V
A
 
I
F
 
F
L
 
L
H
 
H
P
 
P
K
 
K
D
 
D
T
 
C
G
 
G
G
 
G
I
 
V
L
 
L
L
 
V
E
|
E
L
 
L

Query Sequence

>WP_076581962.1 NCBI__GCF_001971705.1:WP_076581962.1
MHFEHAGIATEDAQELAELYSDLFGLAVAHEEEFDGMRVVFLECGDGYFELLEPFEDGTI
AQYLEANGSGIHHLALATEDIEAALETAREHDVSLIDEEPRPGAWGHSVAFLHPKDTGGI
LLELVEH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory