SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_076582810.1 NCBI__GCF_001971705.1:WP_076582810.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
40% identity, 73% coverage: 72:303/317 of query aligns to 62:295/306 of 5mh6A

query
sites
5mh6A

C

C

S

I

S
x
R

A
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A

G
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G

V

Y

G

D

H

E

L

F

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P

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Y

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E

E

E

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M

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N

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x
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R

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G

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x
E

D

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D

L

L

L

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A

A

A

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L

R

D

N

S

E

G

G
x
D

L

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H

A

A

G

A

A

L

L

D

D

V

V

T

F

D
x
S

P

E

E

E

P

P

L

L

P

P

E

E

D

D

H

S

P

P

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W

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D

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D

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H

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I

binding 2-Ketohexanoic acid: R64 (≠ S74), A65 (= A75), G66 (= G76), H89 (= H99), R224 (= R232), H272 (= H280), Y280 (= Y288)
binding magnesium ion: T130 (≠ D138), A132 (≠ H140), F210 (≠ L218), E211 (= E219), M213 (≠ L221), G225 (= G233), P226 (= P234), V228 (= V236), E230 (≠ T238), D241 (≠ G249), S251 (≠ D259)
binding nicotinamide-adenine-dinucleotide: A65 (= A75), G66 (= G76), T86 (≠ S96), H89 (= H99), G142 (= G150), T143 (≠ A151), L144 (≠ I152), R164 (≠ Y172), P196 (= P204), T201 (= T209), V222 (= V230), A223 (≠ G231), R224 (= R232), H272 (= H280), S274 (= S282)

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
40% identity, 73% coverage: 72:303/317 of query aligns to 64:297/308 of 5mhaB

query
sites
5mhaB

C

C

S

I

S
x
R

A
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A

G
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G

V

Y

G

D

H

E

L

F

D

P

L

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E

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T

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F

Y

R

E

E

E

R

A

G

G

I

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A

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N

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D

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P

L

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T

E

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D

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S

A

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A

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D

N

S

E

G

G
x
D

L

I

H

A

A

G

A

A

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L

D

D

V

V

T

F

D

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E

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P

L

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W

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D

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H

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binding 2-Ketohexanoic acid: R66 (≠ S74), R226 (= R232), H274 (= H280), Y282 (= Y288)
binding (2R)-2-hydroxyhexanoic acid: R66 (≠ S74), A67 (= A75), G68 (= G76), H91 (= H99), Y282 (= Y288)
binding magnesium ion: F212 (≠ L218), E213 (= E219), M215 (≠ L221), D243 (≠ G249)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A75), G68 (= G76), T88 (≠ S96), L143 (= L149), G144 (= G150), T145 (≠ A151), L146 (≠ I152), R165 (= R171), R166 (≠ Y172), S167 (= S173), P180 (≠ F186), T197 (≠ C203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H280), S276 (= S282)

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
40% identity, 73% coverage: 72:303/317 of query aligns to 64:297/308 of 5mhaA

query
sites
5mhaA

C

C

S

I

S
x
R

A
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A

G
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G

V

Y

G

D

H

E

L

F

D

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E

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E

E

R

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G

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H

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binding 2-Ketohexanoic acid: R66 (≠ S74), A67 (= A75), G68 (= G76), H91 (= H99), R226 (= R232), H274 (= H280), Y282 (= Y288)
binding magnesium ion: T132 (≠ D138), A134 (≠ H140)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A75), G68 (= G76), T88 (≠ S96), L143 (= L149), G144 (= G150), T145 (≠ A151), L146 (≠ I152), R165 (= R171), R166 (≠ Y172), S167 (= S173), P180 (≠ F186), T197 (≠ C203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H280), S276 (= S282)

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
40% identity, 73% coverage: 72:303/317 of query aligns to 64:297/308 of 5mh5A

query
sites
5mh5A

C

C

S

I

S
x
R

A
|
A

G
|
G

V

Y

G

D

H

E

L

F

D

P

L

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E

G

T

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F

Y

R

E

E

E

R

A

G

G

I

T

A

Y

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L

T

T

N

N

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S

S
x
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H

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G

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N

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A

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L

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G

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A

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G

G

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A

A

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E

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R

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E

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A

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D

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E

D

L

L

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A

A

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x
D

L

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H

A

A

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A

A

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L

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F

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E

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E

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L

L

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L

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I

I

binding 2-Ketohexanoic acid: R66 (≠ S74), A67 (= A75), G68 (= G76), H91 (= H99), R226 (= R232), H274 (= H280), Y282 (= Y288)
binding magnesium ion: T132 (≠ D138), A134 (≠ H140), F212 (≠ L218), E213 (= E219), M215 (≠ L221), D243 (≠ G249)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A75), G68 (= G76), T88 (≠ S96), G142 (= G148), L143 (= L149), G144 (= G150), T145 (≠ A151), L146 (≠ I152), R165 (= R171), R166 (≠ Y172), S167 (= S173), T197 (≠ C203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H280), S276 (= S282)

3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
36% identity, 77% coverage: 59:301/317 of query aligns to 58:298/311 of 3bazA

query
sites
3bazA

E

E

L

L

L

I

E

D

A

A

A

L

E

P

N

K

L

L

Q

E

L

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C

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V

G

G

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F

C

R

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E

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G

G

I

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A

R

V

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T

N

N

A

T

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V

H
x
L

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D

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A

A

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P

P

T

N

D

T

E

N

V

Y

Y

T

G

Y

F

Y

E

G

E

S

I

V

E

V

Q

E

A

L

L

A

V

S

D

N

V

S

D

D

Y

I

L

L

V

V

L

V

A

A

C
|
C

P
|
P

L

L

T

T

D

P

E

E

T
|
T

R

T

G

H

L

I

I

I

G

N

A

R

A

E

E

V

L

I

E

D

T

A

L

L

P

G

P

P

N

K

A

G

V

V

L

L

V

I

N

N

V
x
I

G
|
G

R
|
R

G

G

P

P

L

H

V

V

D

D

T

E

D

P

E

E

L

L

L

V

S

S

A

A

L

L

R

V

N

E

E

G

G

R

L

L

H

G

A

G

A
|
A

A

G

L

L

D
|
D

V

V

T

F

D

E

P

R

E
|
E

P

P

-

E

L

V

P

P

E

E

D

K

H

-

P

-

L

L

W

F

G

G

L

L

G

E

N

N

V

V

Y

V

I

L

T

L

P

P

H
|
H

N

V

S
x
G

G

S

Y

G

T

T

P

V

A

E

Y

T

W

R

E

K

R

V

V

M

A

A

D

D

I

L

L

V

T

V

R

G

N

N

L

L

E

E

active site: L98 (≠ H99), R230 (= R232), A251 (= A253), D254 (= D256), E259 (= E261), H277 (= H280)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ A75), G149 (= G148), L150 (= L149), G151 (= G150), R152 (≠ A151), I153 (= I152), S172 (≠ P174), R173 (≠ E175), S174 (≠ K176), C201 (= C203), P202 (= P204), T207 (= T209), I228 (≠ V230), G229 (= G231), R230 (= R232), D254 (= D256), H277 (= H280), G279 (≠ S282)

Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
36% identity, 77% coverage: 59:301/317 of query aligns to 60:300/313 of Q65CJ7

query
sites
Q65CJ7

E

E

L

L

L

I

E

D

A

A

A

L

E

P

N

K

L

L

Q

E

L

I

F

V

A

S

C

S

S

F

S

S

A

V

G

G

V

L

G

D

H

K

L

V

D

D

L

L

E

I

T

K

F

C

R

E

E

E

R

K

G

G

I

V

A

R

V

V

T

T

N

N

A

T

S

P

G

D

V

V

H

L

G

T

P

D

N

D

I

V

A

A

E

D

H

L

V

A

I

I

G

G

W

L

I

I

L

L

M

A

I

V

T

L

R

R

L

I

D

C

E

E

G

C

I

D

R

K

R

Y

Q

V

E

R

R

R

R

G

E

A

W

W

R

K

H

F

-

G

-

D

F

F

Q

K

A

L

M

T

S

T

D

K

F

F

H

S

D

G

S

K

R

R

V

V

C

G

V

I

G

G

L
|
L

G
|
G

A
x
R

I
|
I

G

G

Q

L

A

A

I

V

L

A

E

E

R

R

L

A

E

E

G

A

F

F

G

D

V

C

E

P

T

-

V

-

G

-

V

I

R

S

Y

Y

S

F

P
x
S

E
x
R

K
x
S

G

K

P

P

T

N

D

T

E

N

V

Y

Y

T

G

Y

F

Y

E

G

E

S

I

V

E

V

Q

E

A

L

L

A

V

S

D

N

V

S

D

D

Y

I

L

L

V

V

L

V

A

A

C

C

P

P

L

L

T

T

D

P

E

E

T

T

R

T

G

H

L

I

I

I

G

N

A

R

A

E

E

V

L

I

E

D

T

A

L

L

P

G

P

P

N

K

A

G

V

V

L

L

V

I

N

N

V
x
I

G

G

R

R

G

G

P

P

L

H

V

V

D

D

T

E

D

P

E

E

L

L

L

V

S

S

A

A

L

L

R

V

N

E

E

G

G

R

L

L

H

G

A

G

A

A

A

G

L

L

D
|
D

V

V

T

F

D

E

P

R

E

E

P

P

-

E

L

V

P

P

E

E

D

K

H

-

P

-

L

L

W

F

G

G

L

L

G

E

N

N

V

V

Y

V

I

L

T

L

P

P

H

H

N

V

S

G

G

S

Y

G

T

T

P

V

A

E

Y

T

W

R

E

K

R

V

V

M

A

A

D

D

I

L

L

V

T

V

R

G

N

N

L

L

E

E

LGRI 152:155 (≠ LGAI 149:152) binding NADP(+)
SRS 174:176 (≠ PEK 174:176) binding NADP(+)
I230 (≠ V230) binding NADP(+)
D256 (= D256) binding NADP(+)

Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
35% identity, 70% coverage: 63:285/317 of query aligns to 71:298/328 of Q9UBQ7

query
sites
Q9UBQ7

A

A

A

G

E

A

N

N

L

L

Q

K

L

V

F

I

A

S

C

T

S

M

S

S

A
x
V

G
|
G

V

I

G

D

H

H

L

L

D

A

L

L

E

D

T

E

F

I

R

K

E

K

R

R

G

G

I

I

A

R

V

V

T

G

N

Y

A

T

S

P

G

D

V

V

H

L

G

T

P

D

N

T

I

T

A

A

E

E

H

L

V

A

I

V

G

S

W

L

I

L

L

L

M

T

I

T

T

C

R

R

L

L

D

P

E

E

G

A

I

I

R

E

Q

V

E

K

R

N

R

G

E

G

W

W

R

T

H

S

F

W

Q

K

A

P

M

L

-

W

-

L

-

C

-

G

S

Y

D

G

F

L

H

T

D

Q

S

S

R

T

V

V

C

G

V

I

G

G

L

L

G
|
G

A
x
R

I
|
I

G

G

Q

Q

A

A

I

I

L

A

E

R

R

R

L

L

E

K

G

P

F

F

G

G

V

V

E

Q

-

R

-

F

-

L

T

Y

V

T

G

G

V
x
R

R
x
Q

Y
x
P

S
x
R

P

P

E

E

K

E

G

A

P

E

T

F

D

Q

E

A

V

-

Y

-

G

E

F

F

E

V

E

S

I

T

E

P

Q

E

A

L

L

A

V

A

D

Q

V

S

D

D

Y

F

L

I

V

V

L

V

A

A

C

C

P
x
S

L

L

T

T

D

P

E

A

T

T

R

E

G

G

L

L

I

C

G

N

A

K

A

D

E

F

L

F

E

Q

T

K

L

M

P

K

P

E

N

T

A

A

V

V

L

F

V

I

N

N

V
x
I

G

S

R
|
R

G

G

P

D

L

V

V

V

D

N

T

Q

D

D

E

D

L

L

L

Y

S

Q

A

A

L

L

R

A

N

S

E

G

G

K

L

I

H

A

A

A

A

G

L

L

D
|
D

V

V

T

T

D

S

P

P

E

E

P

P

L

L

P

P

E

T

D

N

H

H

P

P

L

L

W

L

G

T

L

L

G

K

N

N

V

C

Y

V

I

I

T

L

P

P

H
|
H

N
x
I

S
x
G

G
x
S

Y

A

T

T

VG 83:84 (≠ AG 75:76) binding substrate
GRI 162:164 (≠ GAI 150:152) binding NADP(+)
RQPR 185:188 (≠ VRYS 170:173) binding NADP(+)
S217 (≠ P204) binding NADP(+)
I243 (≠ V230) binding NADP(+)
R245 (= R232) binding substrate
D269 (= D256) binding substrate
HIGS 293:296 (≠ HNSG 280:283) binding substrate
G295 (≠ S282) binding NADP(+)

2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
35% identity, 70% coverage: 63:285/317 of query aligns to 67:294/324 of 2gcgA

query
sites
2gcgA

A

A

A

G

E

A

N

N

L

L

Q

K

L

V

F

I

A

S

C

T

S

M

S
|
S

A
x
V

G
|
G

V

I

G

D

H

H

L

L

D

A

L

L

E

D

T

E

F

I

R

K

E

K

R

R

G

G

I

I

A

R

V

V

T

G

N

Y

A

T

S

P

G

D

V

V

H
x
L

G

T

P

D

N

T

I
x
T

A

A

E

E

H

L

V

A

I

V

G

S

W

L

I

L

L

L

M

T

I

T

T

C

R

R

L

L

D

P

E

E

G

A

I

I

R

E

Q

V

E

K

R

N

R

G

E

G

W

W

R

T

H

S

F

W

Q

K

A

P

M

L

-

W

-

L

-

C

-

G

S

Y

D

G

F

L

H

T

D

Q

S

S

R

T

V

V

C

G

V

I

G
|
G

L

L

G
|
G

A

R

I
|
I

G

G

Q

Q

A

A

I

I

L

A

E

R

R

R

L

L

E

K

G

P

F

F

G

G

V

V

E

Q

-

R

-

F

-

L

T

Y

V

T

G
|
G

V
x
R

R

Q

Y

P

S
x
R

P

P

E

E

K

E

G

A

P

E

T

F

D

Q

E

A

V

-

Y

-

G

E

F

F

E

V

E

S

I

T

E

P

Q

E

A

L

L

A

V

A

D

Q

V

S

D

D

Y

F

L

I

V

V

L

V

A

A

C
|
C

P
x
S

L

L

T

T

D

P

E

A

T
|
T

R

E

G

G

L

L

I

C

G

N

A

K

A

D

E

F

L

F

E

Q

T

K

L

M

P

K

P

E

N

T

A

A

V

V

L

F

V

I

N

N

V
x
I

G

S

R
|
R

G

G

P

D

L

V

V

V

D

N

T

Q

D

D

E

D

L

L

L

Y

S

Q

A

A

L

L

R

A

N

S

E

G

G

K

L

I

H

A

A

A

A

G

L

L

D
|
D

V

V

T

T

D

S

P

P

E
|
E

P

P

L

L

P

P

E

T

D

N

H

H

P

P

L

L

W

L

G

T

L

L

G

K

N

N

V

C

Y

V

I

I

T

L

P

P

H
|
H

N

I

S
x
G

G

S

Y

A

T

T

active site: L103 (≠ H99), R241 (= R232), D265 (= D256), E270 (= E261), H289 (= H280)
binding (2r)-2,3-dihydroxypropanoic acid: S78 (= S74), V79 (≠ A75), G80 (= G76), R241 (= R232), H289 (= H280)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ A75), T107 (≠ I103), G156 (= G148), G158 (= G150), I160 (= I152), G180 (= G169), R181 (≠ V170), R184 (≠ S173), C212 (= C203), S213 (≠ P204), T218 (= T209), I239 (≠ V230), R241 (= R232), D265 (= D256), H289 (= H280), G291 (≠ S282)

Sites not aligning to the query:

binding (2r)-2,3-dihydroxypropanoic acid: 55

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
32% identity, 76% coverage: 59:300/317 of query aligns to 60:308/334 of 5aovA

query
sites
5aovA

E

E

L

V

L

F

E

E

A

N

A

A

E

P

N

R

L

L

Q

R

L

I

F

V

A

A

C

N

S
x
Y

S
x
A

A
x
V

G
|
G

V

Y

G

D

H

N

L

I

D

D

L

V

E

E

T

E

F

A

R

T

E

R

R

R

G

G

I

I

A

Y

V

V

T

T

N

N

A

T

S

P

G

D

V

V

H
x
L

G

T

P

N

N

A

I
x
T

A

A

E

D

H

H

V

A

I

F

G

A

W

L

I

L

L

L

M

A

I

T

T

A

R

R

R

H

L

V

D

V

E

K

G

G

I

D

R

K

R

F

Q

V

E

R

R

S

R

G

E

E

W

W

R

K

-

R

-

K

-

G

-

I

-

A

-

W

-

H

-

P

H

K

F

W

Q

F

A

L

M

G

S

Y

D

E

F

L

H

Y

D

G

S

K

R

T

V

I

C

G

V

I

V

V

G

G

L
x
F

G
|
G

A
x
R

I
|
I

G

G

Q

Q

A

A

I

I

L

A

E

R

R

R

L

A

E

K

G

G

F

F

G

N

V

M

E

R

T

I

V

L

G

-

V

-

R

Y

Y

Y

S
|
S

P
x
R

E

T

K

R

G

K

G

S

P

Q

T

A

D

E

E

K

V

E

Y

L

G

G

-

A

-

E

F

Y

E

R

E

P

I

L

E

E

Q

E

A

V

L

L

V

K

D

E

V

S

D

D

Y

F

L

V

V

I

L

L

A
|
A

C
x
V

P
|
P

L

L

T

T

D

K

E

E

T
|
T

R

M

G

Y

L

M

I

I

G

N

A

E

E

R

L

L

E

K

T

L

L

M

P

K

P

P

N

T

A

A

V

I

L

L

V

V

N

N

V
x
I

G
x
A

R
|
R

G

G

P

K

L

V

V

V

D

D

T

T

D

K

E

A

L

L

L

I

S

K

A

A

L

L

R

K

N

E

E

G

G

W

L

I

H

A

A

G

A

A

A

G

L

L

D
|
D

V

V

T

F

D

E

P

E

E
|
E

P

P

L

Y

P

Y

E

N

D

E

H

E

P

-

L

L

W

F

G

S

L

L

G

D

N

N

V

V

Y

V

I

L

T

T

P

P

H
|
H

N

I

S
x
G

G

S

Y

A

T

T

P

F

A

E

Y

A

W

R

E

E

R

A

V

M

A

A

D

E

I

L

L

V

T

A

R

R

N

N

L

L

active site: L100 (≠ H99), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H280)
binding glyoxylic acid: Y74 (≠ S73), A75 (≠ S74), V76 (≠ A75), G77 (= G76), R241 (= R232), H288 (= H280)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A75), T104 (≠ I103), F158 (≠ L149), G159 (= G150), R160 (≠ A151), I161 (= I152), S180 (= S173), R181 (≠ P174), A211 (= A202), V212 (≠ C203), P213 (= P204), T218 (= T209), I239 (≠ V230), A240 (≠ G231), R241 (= R232), H288 (= H280), G290 (≠ S282)

Sites not aligning to the query:

binding glyoxylic acid: 52, 53, 53

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 76% coverage: 59:300/317 of query aligns to 59:307/332 of 6biiA

query
sites
6biiA

E

E

L

V

L

F

E

D

A

A

E

P

N

R

L

L

Q

R

L

I

F

V

A

A

C

N

S

Y

S

A

A
x
V

G

G

V

Y

G

D

H

N

L

I

D

D

L

I

E

E

T

E

F

A

R

T

E

K

R

R

G

G

I

I

A

Y

V

V

T

T

N

N

A

T

S

P

G

D

V

V

H
x
L

G

T

P

D

N

A

I
x
T

A

A

E

D

H

L

V

A

I

W

G

A

W

L

I

L

L

L

M

A

I

A

T

A

R

R

R

H

L

V

D

V

E

K

G

G

I

D

R

K

R

F

Q

V

E

R

R

S

R

G

E

E

W

W

R

K

-

R

-

G

-

I

-

A

-

W

H

H

F

P

Q

K

A

M

M

F

-

L

-

G

S

Y

D

D

F

V

H

Y

D

G

S

K

R

T

V

I

C

G

V

I

V

V

G
|
G

L
x
F

G
|
G

A
x
R

I
|
I

G

G

Q

Q

A

A

I

I

L

A

E

K

R

R

L

A

E

K

G

G

F

F

G

G

V

M

E

R

T

I

V

L

G

Y

V

T

R
x
A

Y
x
R

S
|
S

P

R

E
x
K

K

P

G

E

G

A

P

E

T

K

D

E

E

L

V

G

Y

A

G

E

F

F

E

K

E

P

I

L

E

E

Q

E

A

L

L

L

V

R

D

E

V

S

D

D

Y

F

L

V

V

V

L

L

A

A

C
x
V

P
|
P

L

L

T

T

D

K

E
|
E

T
|
T

R

Y

G

H

L

M

I

I

G

N

A

E

E

R

L

L

E

R

T

L

L

M

P

K

P

P

N

T

A

A

V

V

L

L

V

V

N

N

V
|
V

G
x
A

R
|
R

G

G

P

K

L

V

V

V

D

D

T

T

D

K

E

A

L

L

L

I

S

R

A

A

L

L

R

K

N

E

E

G

G

W

L

I

H

A

A

A

A

G

L

L

D
|
D

V

V

T

F

D

E

P

E

E
|
E

P

P

L

Y

P

-

E

Y

D

D

H

E

P

E

L

L

W

F

G

A

L

L

G

D

N

N

V

V

Y

V

I

L

T

T

P

P

H
|
H

N

I

S
x
G

G

S

Y

A

T

T

P

F

A

G

Y

A

W

R

E

E

R

G

V

M

A

A

D

E

I

L

L

V

T

A

R

K

N

N

L

L

active site: L99 (≠ H99), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H280)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A75), T103 (≠ I103), G156 (= G148), F157 (≠ L149), G158 (= G150), R159 (≠ A151), I160 (= I152), A179 (≠ R171), R180 (≠ Y172), S181 (= S173), K183 (≠ E175), V211 (≠ C203), P212 (= P204), E216 (= E208), T217 (= T209), V238 (= V230), A239 (≠ G231), R240 (= R232), D264 (= D256), H287 (= H280), G289 (≠ S282)

6ttbA Crystal structure of NAD-dependent formate dehydrogenase from staphylococcus aureus in complex with NAD
31% identity, 85% coverage: 47:317/317 of query aligns to 54:329/331 of 6ttbA

query
sites
6ttbA

D

D

A

M

A

D

V

V

V

I

G

S

R

A

D

P

L

F

R

Y

P

P

-

A

-

Y

-

M

-

T

-

R

E

E

L

R

L

I

E

E

A

K

A

A

E

P

N

N

L

L

Q

K

L

L

F

A

A

I

C

T

S

A

S

G

A
x
V

G

G

V

S

G

D

H

H

L

V

D

D

L

L

E

A

T

A

F

A

R

S

E

E

R

H

G

N

I

I

A

G

V

V

T

V

N

E

A

V

S

T

G

G

V

S

H
x
N

G

T

P

V

N

S

I
x
V

A

A

E

E

H

H

V

A

I

V

G

M

W

D

I

L

L

L

M

I

I

L

T

L

R

R

R

N

L

Y

D

E

E

E

G

G

I

H

R

R

R

Q

Q

S

E

V

R

E

R

G

E

E

W

W

R

N

H

L

F

S

Q

Q

A

V

M

G

S

N

D

H

F

A

H

H

D

E

-

L

-

Q

-

H

S

K

R

T

V

I

C

G

V

I

V
x
F

G

G

L

F

G
|
G

A
x
R

I
|
I

G

G

Q

Q

A

L

I

V

L

A

E

E

R

R

L

L

E

A

G

P

F

F

G

N

V

V

E

T

T

L

V

Q

G

-

V

-

R

H

Y
|
Y

S
x
D

P
|
P

-

I

-

N

E

Q

K

Q

G

D

G

H

P

K

T

L

D

S

E

K

V

F

Y

V

G

S

F

F

E

D

E

E

I

L

E

-

Q

-

A

-

L

V

V

S

D

S

V

S

D

D

Y

A

L

I

V

T

L

I

A
x
H

C
x
A

P
|
P

L

L

T

T

D

P

E

E

T
|
T

R

D

G

N

L

L

I

F

G

D

A

K

A

D

E

V

L

L

E

S

T

R

L

M

P

K

N

H

A

S

V

Y

L

L

V

V

N

N

V
x
T

G
x
A

R
|
R

G

G

P

K

L

I

V

V

D

N

T

R

D

D

E

A

L

L

L

V

S

E

A

A

L

L

R

A

N

S

E

E

G

H

L

L

H

Q

A

G

A

Y

A

A

L

G

D

D

V

V

T

W

D

Y

P

P

E

Q

P

P

L

A

P

P

E

A

D

D

H

H

P

P

L

W

W

R

G

T

L

M

G

P

N

R

V

N

Y

A

I

M

T

T

P

V

H
|
H

N

Y

S
|
S

G
|
G

Y

M

T

T

P

L

A

E

Y

A

W

Q

E

K

R

R

V

I

A

E

D

D

I

G

L

V

T

K

R

D

N

I

L

L

E

E

R

R

I

F

D

F

E

N

D

H

G

E

P

P

D

F

A

Q

D

D

L

K

E

D

N

I

Q

I

V

V

A

A

binding nicotinamide-adenine-dinucleotide: V87 (≠ A75), N111 (≠ H99), V115 (≠ I103), F162 (≠ V147), G165 (= G150), R166 (≠ A151), I167 (= I152), Y185 (= Y172), D186 (≠ S173), P187 (= P174), H214 (≠ A202), A215 (≠ C203), P216 (= P204), T221 (= T209), T242 (≠ V230), A243 (≠ G231), R244 (= R232), H292 (= H280), S294 (= S282), G295 (= G283)

1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
30% identity, 79% coverage: 43:291/317 of query aligns to 37:289/304 of 1wwkA

query
sites
1wwkA

E

E

L

L

V

V

R

K

D

D

A

V

A

E

V

A

V

I

G

V

R

R

D

S

L

-

R

K

P

P

E

K

L

V

-

T

-

R

-

V

L

I

E

E

A

S

A

A

E

P

N

K

L

L

Q

K

L

V

F

I

A

A

C

R

S

A

S

G

A

V

G

G

V

L

G

D

H

N

L

I

D

D

L

V

E

E

T

A

F

A

R

K

E

E

R

K

G

G

I

I

A

E

V

V

T

V

N

N

A

A

S

P

G

A

V

A

H
x
S

G

S

P

R

N

S

I
x
V

A

A

E

E

H

L

V

A

I

V

G

G

W

L

I

M

L

F

M

S

I

V

T

A

R

R

R

K

L

I

D

A

E

F

G

A

I

D

R

R

R

K

Q

M

E

R

R

E

R

G

E

V

W

W

R

A

H

K

F

K

Q

E

A

A

M

M

S

G

-

I

D

E

F

L

H

E

D

G

S

K

R

T

V

I

C

G

V

I

G
|
G

L
x
F

G
|
G

A
x
R

I
|
I

G

G

Q

Y

A

Q

I

V

L

A

E

K

R

I

L

A

E

N

G

A

F

L

G

G

V

M

E

N

T

I

V

L

G

L

V
x
Y

R
x
D

Y
x
P

S

Y

P

P

E

N

K

E

G

E

G

R

P

A

T

K

D

E

E

V

V

N

Y

G

G

K

F

F

E

V

E

D

I

L

E

E

Q

T

A

L

L

L

V

K

D

E

V

S

D

D

Y

V

L

V

V

T

L

I

A

H

C
x
V

P
|
P

L

L

T

V

D

E

E

S

T
|
T

R

Y

G

H

L

L

I

I

G

N

A

E

E

R

L

L

E

K

T

L

L

M

P

K

N

T

A

A

V

I

L

L

V

I

N

N

V
x
T

G
x
S

R
|
R

G

G

P

P

L

V

V

V

D

D

T

T

D

N

E

A

L

L

L

V

S

K

A

A

L

L

R

K

N

E

E

G

G

W

L

I

H

A

A

G

A

A

A

G

L

L

D
|
D

V

V

T

F

D

E

P

E

E
|
E

P

P

L

L

P

P

E

K

D

D

H

H

P

P

L

L

W

T

G

K

L

F

G

D

N

N

V

V

Y

V

I

L

T

T

P

P

H
|
H

N

I

S
x
G

G

A

Y

S

T

T

P

V

A

E

Y

A

W

Q

E

E

R

R

active site: S96 (≠ H99), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H280)
binding nicotinamide-adenine-dinucleotide: V100 (≠ I103), G146 (= G148), F147 (≠ L149), G148 (= G150), R149 (≠ A151), I150 (= I152), Y168 (≠ V170), D169 (≠ R171), P170 (≠ Y172), V201 (≠ C203), P202 (= P204), T207 (= T209), T228 (≠ V230), S229 (≠ G231), D254 (= D256), H278 (= H280), G280 (≠ S282)

5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
31% identity, 88% coverage: 29:308/317 of query aligns to 19:330/336 of 5z20F

query
sites
5z20F

D

D

A

S

D

E

V

S

R

F

Q

Q

A

A

S

S

S

N

R

H

R

R

-

H

-

G

-

F

-

E

-

L

-

H

-

F

E

Q

R

A

E

H

L

L

V

Q

R

A

D

D

A

T

A

A

V

V

V

L

-

A

-

Q

-

G

-

F

-

E

-

V

-

C

-

A

-

F

V

V

G

N

R

D

D

D

L

L

R

S

P

R

E

P

L

V

L

L

E

E

-

R

-

L

A

A

E

G

N

G

L

T

Q

R

L

L

F

V

A

A

C

L

S

R

S

S

A

A

G

G

V

Y

G

N

H

H

L

V

D

D

L

L

E

A

T

A

F

A

R

E

E

A

R

L

G

G

I

L

A

P

V

V

T

V

N

H

A

V

S

P

G

A

V
x
Y

H
x
S

G

P

P

H

N

A

I

V

A

A

E

E

H

H

V

A

I

V

G

G

W

L

I

I

L

L

M

T

I

L

T

N

R

R

L

L

D

H

E

R

G

A

I

Y

R

N

R

R

Q

T

E

R

R

E

R

G

E

D

W

F

R

S

-

L

H

H

F

G

Q

L

A

T

M

G

S

F

D

D

F

L

H

H

D

G

S

K

R

R

V

V

C

G

V

V

V

I

G

G

L

T

G
|
G

A
x
Q

I
|
I

G

G

Q

E

A

T

I

F

L

A

E

R

R

I

L

M

E

A

G

G

F

F

G

G

V

C

E

E

T

L

V

L

G

A

V

-

R

-

Y
|
Y

S
x
D

P
|
P

E

Y

K

P

G

N

G

-

P

P

T

R

D

I

E

Q

V

A

Y

L

G

G

-

G

-

R

F

Y

E

L

E

A

I

L

E

D

Q

A

A

L

L

L

V

A

D

E

V

S

D

D

Y

I

L

V

V

S

L

L

A

H

C
|
C

P
|
P

L

L

T

T

D

A

E

D

T
|
T

R

R

G

H

L

L

I

I

G

D

A

A

A

Q

E

R

L

L

E

A

T

T

L

M

P

K

P

P

N

G

A

A

V

M

L

L

V

I

N

N

V
x
T

G
|
G

R
|
R

G

G

P

A

L

L

V

V

D

N

T

A

D

A

E

A

L

L

L

I

S

E

A

A

L

L

R

K

N

S

E

G

G

Q

L

L

H

G

A

Y

A

L

A

G

L

L

D
|
D

V

V

T

Y

D

E

P

E

E
|
E

-

A

-

D

-

I

-

F

-

E

-

D

-

R

-

S

-

D

-

Q

P

P

L

L

P

Q

E

D

D

D

-

V

-

L

H

A

P

R

L

L

W

L

G

S

L

F

G

P

N

N

V

V

Y

V

I

V

T

T

P

A

H
|
H

N

Q

S
x
A

G

F

Y

L

T

T

P

R

A

E

Y

A

W

L

E

A

R

A

V

I

A

A

D

D

I

T

L

T

T

L

R

D

N

N

L

I

E

A

R

A

I

W

D

Q

E

D

D

G

G

T

P

P

active site: S108 (≠ H99), R241 (= R232), D265 (= D256), E270 (= E261), H302 (= H280)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (≠ V98), G160 (= G150), Q161 (≠ A151), I162 (= I152), Y180 (= Y172), D181 (≠ S173), P182 (= P174), C212 (= C203), P213 (= P204), T218 (= T209), T239 (≠ V230), G240 (= G231), R241 (= R232), H302 (= H280), A304 (≠ S282)

7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
29% identity, 82% coverage: 41:301/317 of query aligns to 66:340/361 of 7arzA

query
sites
7arzA

E

D

R

K

E

E

L

L

V

A

R

-

D

D

A

A

A

H

V

I

V

L

V

I

G
x
T

R

T

D
x
P

L
x
F

R

H

P

P

-

A

-

Y

-

M

-

T

-

K

E

E

L

R

L

L

E

A

A

K

A

A

E

K

N

N

L

L

Q

E

L

L

F
x
L

A
x
V

C
x
T

S

A

S
x
G

A
x
V

G

G

V

S

G

D

H

H

L

I

D

D

L

L

E

H

T

A

F

A

R

A

E

E

R

K

G

G

I

L

A

T

V

V

T

S

N

E

A

V

S

T

G

G

V

S

H
x
N

G

V

P

T

N

S

I
x
V

A

A

E

E

H

D

V

E

I

V

G

L

W

R

I

I

L

L

M

V

I

L

T

V

R

R

R

N

L

F

D

A

E

P

G

G

I

W

R

K

R

Q

Q

V

E

S

R

E

R

G

E

G

W

W

R

N

H

V

F

A

Q

A

A

V

M

V

S

H

-

H

-

A

-

Y

D

D

F

L

H

I

D

D

S

R

R

T

V

V

C

G

V

T

V

V

G

G

L

G

G
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G

A
x
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I
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I

G

G

Q

Q

A

E

I

L

L

M

E

K

R

R

L

L

E

K

G

G

F

F

G

G

V

L

E

K

T

E

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M

G

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V

Y

R

Y

Y
x
D

S
x
R

P

N

E

S

K

L

G

G

G

A

P

E

T

R

D

E

E

K

V

E

Y

L

G

G

F

C

E

K

-

R

-

E

-

T

E

D

I

L

E

D

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T

A

M

L

L

V

S

D

K

V

C

D

D

Y

V

L

V

V

V

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V

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N

C
x
T

P
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P

L

L

T

T

D

D

E
x
Q

T

T

R

R

G

G

L

L

I

F

G

N

A

K

A

E

E

R

L

I

E

A

T

K

L

M

P

K

N

G

A

A

V

Y

L

L

V

V

N

N

V
x
N

G
x
A

R
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R

G

G

P

A

L

I

V

A

D

D

T

T

D

E

E

A

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L

K

S

E

A

A

L

C

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E

N

S

E

G

G

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H

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A

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Y

A

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D

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P

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H

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H

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S

G
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G

Y

T

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T

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L

A

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Y

A

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K

R

R

V

F

A

A

-

A

-

G

-

T

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K

D

D

I

M

L

I

T

D

R

R

N

W

L

L

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K

binding azide ion: T77 (≠ G53), P79 (≠ D55), F80 (≠ L56), L99 (≠ F70), V100 (≠ A71), T101 (≠ C72), G103 (≠ S74), V104 (≠ A75), R267 (= R232), H315 (= H280)
binding nicotinamide-adenine-dinucleotide: V104 (≠ A75), N128 (≠ H99), V132 (≠ I103), G182 (= G150), R183 (≠ A151), I184 (= I152), D204 (≠ Y172), R205 (≠ S173), T238 (≠ C203), P239 (= P204), Q243 (≠ E208), N265 (≠ V230), A266 (≠ G231), R267 (= R232), D291 (= D256), H315 (= H280), S317 (= S282), G318 (= G283)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 359

Q08911 Formate dehydrogenase 1; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 83% coverage: 41:302/317 of query aligns to 59:351/376 of Q08911

query
sites
Q08911

E

D

R

R

E

E

L

L

V

-

R

K

D

D

A

A

A

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T

D

P

L

F

R

F

P

P

E

A

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Y

L

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S

-

R

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N

-

R

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I

A

A

E

E

N

A

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Q

N

L

L

F

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A

L

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S

T

A

A

G

G

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V

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G

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D

H

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D

D

L

L

E

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A

F

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E

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I

A

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N

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A

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T

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I

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Y

D

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Y

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E

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R

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L

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F

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K

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x
D

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x
Y

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E

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A

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A

A

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Y

L

L

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N

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A

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A

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L

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A

A

L

V

R

K

N

S

E

G

G

K

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L

H

A

A

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Y

A

G

L

G

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D

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W

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P

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K

D

D

H

H

P

P

L

W

W

R

G

T

L

M

G

D

N

N

V

K

-

D

-

H

-

V

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G

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N

Y

A

I

M

T

T

P

V

H

H

N

I

S

S

G

G

Y

T

T

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L

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-

A

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Q

P

K

A

R

Y

Y

W

A

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L

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N

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K

DY 197:198 (vs. gap) mutation to AR: Shifts the coenzyme preference of the enzyme from NAD(+) to NADP(+).

P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 84% coverage: 42:307/317 of query aligns to 48:319/410 of P0A9T0

query
sites
P0A9T0

R

K

E

E

L

S

V

I

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R

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D

A

A

A

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A

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N

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E

E

G

A

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R

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F

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x
H

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I

G

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T

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Q

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L

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E

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R

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L

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E

-

G

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G

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V

A

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E

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V

L

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G

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D

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I

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E

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Q

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Y

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H

C

V

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P

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L

M

I

M

G

G

A

A

A

K

E

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S

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L

L

M

P

K

P

P

N

G

A

S

V

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L

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I

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x
A

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x
S

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R

G

G

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T

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V

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D

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P

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A

L

L