SitesBLAST
Comparing WP_077243567.1 NCBI__GCF_001995255.1:WP_077243567.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
66% identity, 99% coverage: 1:1245/1263 of query aligns to 1:1225/1227 of P13009
- M1 (= M1) modified: Initiator methionine, Removed
- C247 (= C247) binding Zn(2+)
- C310 (= C310) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- C311 (= C311) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- E694 (= E692) binding methylcob(III)alamin
- GDVHD 756:760 (= GDVHD 754:758) binding methylcob(III)alamin
- D757 (= D755) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
- H759 (= H757) binding axial binding residue; mutation to G: Loss of catalytic activity.
- S804 (= S802) binding methylcob(III)alamin
- T808 (= T806) binding methylcob(III)alamin
- S810 (= S808) mutation to A: Decreases activity by about 40%.
- A860 (= A858) binding methylcob(III)alamin
- D946 (= D964) binding S-adenosyl-L-methionine
- R1134 (= R1154) binding S-adenosyl-L-methionine
- YY 1189:1190 (≠ YF 1209:1210) binding S-adenosyl-L-methionine
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
54% identity, 98% coverage: 5:1245/1263 of query aligns to 19:1263/1265 of Q99707
- R61 (≠ S47) natural variant: R -> K
- C255 (≠ S242) to Y: in dbSNP:rs1140598
- GSR 382:384 (= GSR 367:369) binding (6S)-5,6,7,8-tetrahydrofolate
- D449 (= D434) binding (6S)-5,6,7,8-tetrahydrofolate
- N470 (= N455) binding (6S)-5,6,7,8-tetrahydrofolate
- D537 (= D522) binding (6S)-5,6,7,8-tetrahydrofolate
- N579 (= N564) binding (6S)-5,6,7,8-tetrahydrofolate
- R585 (= R570) binding (6S)-5,6,7,8-tetrahydrofolate
- R591 (= R576) binding (6S)-5,6,7,8-tetrahydrofolate
- D919 (≠ Q891) to G: in dbSNP:rs1805087
- D963 (= D953) mutation to E: Decreases binding to MTRR; when associated with N-1071.
- K1071 (= K1054) mutation to N: Decreases binding to MTRR; when associated with E-963.
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
60% identity, 47% coverage: 651:1245/1263 of query aligns to 3:575/576 of 3ivaA
- active site: D107 (= D755), H109 (= H757), S160 (= S808)
- binding cobalamin: H109 (= H757), G112 (= G760), V116 (= V764), G152 (= G800), L153 (= L801), S154 (= S802), L156 (= L804), I157 (= I805), T158 (= T806), G183 (= G831), G184 (= G832), Q208 (≠ K856), N209 (≠ D857), T303 (≠ S971), D443 (= D1113), A486 (= A1156), G488 (= G1158), Y489 (= Y1159), H495 (= H1165), A520 (= A1190), M521 (= M1191), G524 (≠ V1194), V527 (= V1197), S528 (= S1198)
- binding s-adenosyl-l-homocysteine: E447 (= E1117), R484 (= R1154), P485 (= P1155), Y489 (= Y1159), A491 (= A1161), Y539 (= Y1209)
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
60% identity, 47% coverage: 651:1245/1263 of query aligns to 3:575/577 of 3bulA
- active site: D107 (= D755), H109 (= H757), S160 (= S808)
- binding cobalamin: H109 (= H757), V116 (= V764), G152 (= G800), L153 (= L801), S154 (= S802), L156 (= L804), I157 (= I805), T158 (= T806), G183 (= G831), G184 (= G832), Q208 (≠ K856), N209 (≠ D857), A210 (= A858), T213 (≠ A861), M302 (≠ H970), D443 (= D1113), A486 (= A1156), P487 (= P1157), G488 (= G1158), Y489 (= Y1159), H495 (= H1165), K498 (= K1168), M521 (= M1191), G524 (≠ V1194), V527 (= V1197), S528 (= S1198)
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
55% identity, 50% coverage: 5:636/1263 of query aligns to 3:611/611 of 4cczA
- binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E361), G342 (= G367), R344 (= R369), N430 (= N455), M458 (= M483), D497 (= D522), G536 (= G561), S538 (= S563), N539 (= N564), F542 (= F567), R545 (= R570), R551 (= R576)
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
35% identity, 66% coverage: 10:840/1263 of query aligns to 7:806/841 of 8g3hA