SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_077275372.1 NCBI__GCF_001998885.1:WP_077275372.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AB74 D-tagatose-1,6-bisphosphate aldolase subunit KbaY; TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Ketose 1,6-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase; EC 4.1.2.40 from Escherichia coli (strain K12) (see paper)
37% identity, 89% coverage: 1:248/278 of query aligns to 1:251/286 of P0AB74

query
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P0AB74

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D82 (= D82) active site, Proton donor
H83 (= H83) binding Zn(2+)
H180 (= H180) binding Zn(2+)
H208 (= H208) binding Zn(2+)

1gvfB Structure of tagatose-1,6-bisphosphate aldolase (see paper)
37% identity, 88% coverage: 3:248/278 of query aligns to 2:242/275 of 1gvfB

query
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1gvfB

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active site: D81 (= D82), H82 (= H83), H171 (= H180), H199 (= H208), N221 (= N230)
binding phosphoglycolohydroxamic acid: D81 (= D82), H82 (= H83), H171 (= H180), G172 (= G181), H199 (= H208), G200 (= G209), S202 (= S211), N221 (= N230), V222 (≠ I231), A223 (≠ F232), T224 (= T233)
binding zinc ion: H82 (= H83), H171 (= H180), H199 (= H208)

3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
38% identity, 89% coverage: 1:247/278 of query aligns to 1:252/285 of 3q94A

query
sites
3q94A

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active site: D85 (= D82), H86 (= H83), E145 (= E145), H181 (= H180), H209 (= H208), N231 (= N230)
binding zinc ion: H86 (= H83), E114 (≠ A111), H163 (≠ T162), H181 (= H180), H209 (= H208), E235 (vs. gap), E239 (≠ D234)

3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
37% identity, 84% coverage: 2:235/278 of query aligns to 1:258/307 of 3n9sA

query
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3n9sA

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active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H180 (= H180), A226 (≠ S212), N253 (= N230)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (≠ A50), D82 (= D82), H83 (= H83), H180 (= H180), G181 (= G181), K184 (vs. gap), H210 (= H208), G211 (= G209), S213 (= S211), N253 (= N230), D255 (≠ F232), T256 (= T233)
binding zinc ion: H83 (= H83), H180 (= H180), H210 (= H208)

P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
37% identity, 96% coverage: 1:266/278 of query aligns to 1:272/285 of P13243

query
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P13243

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D

V

P

S

K

D

E

A

C

V

Q

T

E

F

L

V

V

E

E

E

R

T

T

Q

G

V

I

D

D

S

C

L

L

A

A

I

P

S

A

I

L

G

G

T

S

A

V

H

H

G

G

A

P

Y

Y

K

K

G

G

E

E

P

P

K

N

I

L

N

G

F

F

D

K

R

E

L

M

I

E

D

E

I

I

A

G

K

K

Q

S

V

T

D

G

I

L

P

P

L

L

V

V

L

L

H

H

G

G

S
x
T

S

G

S

I

G

P

D

T

E

A

N

D

L

I

K

K

R

K

C

S

A

I

V

S

T

L

G

G

I

T

E

A

K

K

I

I

N

N

I

V

F

N

T
|
T

D

E

F

N

I

Q

N

I

S

S

A

S

F

A

E

K

A

A

I

V

T

R

-

E

-

T

-

L

S

A

E

A

Q

K

P

P

T

D

D

E

Y

Y

L

D

Q

P

V

R

K

K

-

Y

-

L

A

G

V

P

A

A

N

R

Q

E

A

A

I

I

E

K

K

E

T

T

L

V

T212 (≠ S211) modified: Phosphothreonine
T234 (= T233) modified: Phosphothreonine

1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
36% identity, 98% coverage: 2:273/278 of query aligns to 1:299/305 of 1rv8B

query
sites
1rv8B

V

M

L

L

V

V

N

T

S

G

K

L

E

E

L

I

F

L

K

K

A

A

R

R

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

V

F

N

D

N

L

M

D

E

S

F

A

L

R

Q

S

A

Y

V

V

L

A

E

V

A

A

A

E

E

R

E

E

Q

N

R

K

S

P

P

L

V

I

I

L

L

A

A

L

L

A

S

E

E

A

G

H

-

L

-

D

-

M

-

I

-

S

A

L

M

E

K

E

Y

G

G

A

G

-

R

-

A

-

L

-

T

L

L

I

M

G

A

K

V

Y

E

L

L

A

A

E

K

K

E

A

A

T

R

V

V

P

P

V

V

V

A

L

V

H

H

L

L

D
|
D

H
|
H

G

G

Q

S

T

S

L

Y

S

E

V

S

I

V

E

L

R

R

A

A

I

L

D

R

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

K

S

S

E

H

S

E

T

D

F

F

A

E

E

T

N

N

V

V

A

R

L

E

T

T

K
x
R

K

R

V

V

A

E

M

A

A

A

K
x
H

G

A

K

V

N

G

V

V

A

T

V

V

E

E

A

A

E
|
E

I

L

G

G

H

R

V

L

G

A

S

G

G

I

V
x
E

N

E

Y

H

E

V

N

A

H

V

E

D

V

E

K

K

D

D

S

A

I

L

Y

L

T

T

E

N

V

P

S

E

D

E

A

A

V

R

T

I

F

F

V

M

E

E

E

R

T

T

Q

G

V

A

D

D

S

Y

L

L

A

A

I

V

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

A

Y

Y

K

K

G

G

E

K

-

G

-

R

P

P

K

F

I

I

N

D

F

H

D

A

R

R

L

L

I

E

D

R

I

I

A

A

K

R

Q

L

V

V

D

P

I

A

P

P

L

L

V

V

L

L

H
|
H

G

G

G

A

S
|
S

-

A

-

V

-

P

-

E

-

L

-

V

-

E

-

R

-

F

-

R

-

A

S

S

S

G

G

G

D

E

-

I

-

G

-

E

-

A

-

G

-

I

-

H

-

P

E

E

N

D

L

I

K

K

R

K

C

A

A

I

V

S

T

L

G

G

I

I

E

A

K

K

I

I

N
|
N

I

T

F
x
D

T
|
T

D

D

F

-

I

L

N

R

S

L

A

A

F

F

E

T

A

A

I

L

T

I

S

R

E

E

-

A

-

L

-

N

-

K

-

N

-

P

-

K

-

E

-

F

Q

D

P

P

T

R

D

K

Y

Y

L

L

Q

-

V

-

K

-

A

G

V

P

A

A

N

R

Q

E

A

A

I

V

E

K

K

E

T

V

L

V

S

K

H

S

Y

R

Y

M

Q

E

V

L

F

F

active site: D80 (= D82), H81 (= H83), E140 (≠ V142), H178 (= H180), H208 (= H208), N251 (= N230)
binding cobalt (ii) ion: H81 (= H83), E132 (= E134), H178 (= H180), H208 (= H208)
binding sulfate ion: R116 (≠ K118), H123 (≠ K125), S211 (= S211), D253 (≠ F232), T254 (= T233)

8q59A Crystal structure of metal-dependent class ii sulfofructose phosphate aldolase from yersinia aldovae in complex with sulfofructose phosphate (yasqia-zn-sfp) (see paper)
35% identity, 91% coverage: 1:252/278 of query aligns to 1:247/280 of 8q59A

query
sites
8q59A

M

M

V

S

L

Y

V

V

N

S

S

G

K

N

E

T

L

L

F

I

K

Q

K

H

A

A

R

W

E

Q

E

H

Q

G

Y

Y

A

A

I

I

P

G

A

A

C

F

N

S

F

V

F

H

D

N

L

A

D

E

S

T

A

I

R

R

S

A

Y

I

V

L

A

L

V

A

A

A

E

E

R

Q

E

E

N

Q

K

A

P

P

L

V

I

M

L

L

A

Q

L

I

A
x
G

E
x
Q

A
x
K

H

V

L

I

D

S

M

V

I

M

S

G

L

L

E

K

E

P

G

M

A

K

L

E

I

M

G

I

K

D

Y

A

L

F

A

M

E

H

K

D

A

I

T

T

V

V

P

P

V

V

V

C

L

I

H

H

L

L

D
|
D

H
|
H

G

S

Q

R

T

S

L

F

S

E

V

Q

I

T

E

M

R

E

A

A

I

V

D

Q

L

A

D

G

F

F

T

Q

S

S

V

V

M

M

I

F

D

D

K

G

S

S

E

H

S

L

T

S

F

F

A

D

E

E

N

N

V

V

A

R

L

I

T

T

K

R

K

A

V

V

V

A

A

D

M

V

A

A

K

H

G

A

K

L

N

N

V

L

A

G

V

V

E

E

A

G

E

E

I

I

G

G

H

K

V

I

G

G

S

G

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

T

K

E

D

D

S

A

I

L

Y

I

T

T

E

S

V

C

S

A

D

E

A

A

V

L

T

K

F

F

V

S

E

E

E

L

T

T

Q

T

V

V

D

D

S

Y

L

L

A

A

I

V

S

S

I

I

G

G

T

T

A

A

H
|
H

G
|
G

A

M

Y

Y

K

K

G

Q

E

E

P

P

K

K

I

L

N

A

F

F

D

E

R

R

L

L

I

Q

D

E

I

M

A

R

K

E

Q

I

V

V

D

K

I

K

P

P

L

I

V

V

L

L

H
|
H

G
|
G

G

G

S
|
S

S

G

S

V

G

P

D

D

E

E

N

Q

L

I

K

R

R

R

C

A

A

I

V

T

T

L

G

G

I

V

E

A

K

K

I

V

N
|
N

I

V

F
x
D

T
|
T

D

E

F

-

I

L

N

R

S

Q

A

A

F

F

E

T

A

Q

I

G

T

V

S

S

E

E

-

V

-

L

-

A

Q

S

P

P

T

D

D

E

Y

Y

L

V

binding (3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-oxidanylidene-6-phosphonooxy-hexane-1-sulfonic acid: G50 (≠ A50), Q51 (≠ E51), K52 (≠ A52), D82 (= D82), H83 (= H83), H172 (= H180), G173 (= G181), H200 (= H208), G201 (= G209), S203 (= S211), N222 (= N230), D224 (≠ F232), T225 (= T233)
binding zinc ion: H83 (= H83), H172 (= H180), H200 (= H208)

8q5aA Crystal structure of metal-dependent class ii sulfofructosephosphate aldolase from hafnia paralvei hpsqia-zn in complex with dihydroxyacetone phosphate (dhap) (see paper)
34% identity, 90% coverage: 1:251/278 of query aligns to 1:246/276 of 8q5aA

query
sites
8q5aA

M

M

V

P

L

Y

V

V

N

S

S

G

K

K

E

T

L

M

F

I

K

Q

A

A

R

W

E

K

E

H

Q

G

Y

Y

A

A

I

I

P

G

A

A

C

F

N

S

F

A

F

H

D

N

L

A

D

E

S

T

A

V

R

R

S

A

Y

I

V

L

A

L

V

A

A

A

E

Q

R

E

E

E

N

Q

K

S

P

P

L

V

I

M

L

I

A

Q

L

V

A

G

E

Q

A

K

H

V

L

I

D

S

M

V

I

M

S

G

L

L

E

K

E

P

G

M

A

K

L

E

I

I

G

I

K

D

Y

A

L

F

A

M

E

H

K

D

A

I

T

T

V

V

P

P

V

V

V

C

L

I

H

H

L

L

D

D

H
|
H

G

S

Q

R

T

S

L

F

S

E

V

Q

I

T

E

M

R

Q

A

A

I

I

D

Q

L

C

D

G

F

F

T

Q

S

S

V

V

M

M

I

F

D

D

K

G

S

S

E

H

S

L

T

S

F

F

A

E

E

E

N

N

V

V

A

R

L

I

T

T

K

R

K

A

V

A

V

S

A

E

M

V

A

A

K

H

G

A

K

L

N

N

V

I

A

G

V

M

E

E

A

G

E

E

I

I

G

G

H

K

V

I

G

G

S

G

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

T

K

K

D

D

S

A

I

L

Y

I

T

T

E

S

V

C

S

D

D

E

A

A

V

L

T

R

F

F

V

S

E

E

E

G

T

T

Q

D

V

V

D

D

S

Y

L

L

A

A

I

V

S

S

I

I

G

G

T

T

A

A

H
|
H

G

G

A

V

Y

Y

K

K

G

Q

E

E

P

P

K

K

I

L

N

A

F

F

D

G

R

R

L

L

I

Q

D

E

I

I

A

R

K

E

Q

I

V

V

D

K

I

K

P

P

L

I

V

V

L

L

H
|
H

G

G

S

S

S

G

S

V

G

P

D

D

E

D

N

Q

L

I

K

R

R

Q

C

A

A

I

V

A

T

L

G

G

I

V

E

A

K

K

I

V

N

N

I

V

F

D

T

T

D

E

-

L

-

R

-

Q

-

A

F

F

I

T

N

Q

S

G

A

L

F

C

E

E

A

V

I

L

T

T

S

N

E

D

Q

-

P

P

T

E

D

E

Y

Y

binding zinc ion: H83 (= H83), H83 (= H83), H172 (= H180), H200 (= H208), H200 (= H208)

3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
36% identity, 84% coverage: 2:235/278 of query aligns to 1:248/297 of 3n9rA

query
sites
3n9rA

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A

S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A
x
C

E
|
E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D

D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G
|
G

H

R

V

L

G

-

S

M

G

G

V

I

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

A

I

V

Y

L

T

V

E

N

V

P

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G
|
G

A

A

-

F

-
x
K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G
|
G

G

A

S
|
S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S
x
A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N
|
N

I

T

F
x
D

T
|
T

D

D

F

L

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H170 (= H180), A216 (≠ S212), N243 (= N230)
binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H83), H170 (= H180), G171 (= G181), K174 (vs. gap), H200 (= H208), G201 (= G209), S203 (= S211), N243 (= N230), D245 (≠ F232), T246 (= T233)
binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H208)

3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
36% identity, 84% coverage: 2:235/278 of query aligns to 1:248/297 of 3c56A

query
sites
3c56A

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N
|
N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A
x
S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A
x
C

E
|
E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G
|
G

H

R

V

L

G

-

S

M

G

G

V

I

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

A

I

V

Y

L

T

V

E

N

V

P

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

A

-

F

-
x
K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G
|
G

G

A

S
|
S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S
x
A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N
|
N

I

T

F
x
D

T
|
T

D

D

F

L

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H170 (= H180), A216 (≠ S212), N243 (= N230)
binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (≠ A50), D82 (= D82), H170 (= H180), K174 (vs. gap), G201 (= G209), S203 (= S211), N243 (= N230), D245 (≠ F232), T246 (= T233)
binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H208)

Sites not aligning to the query:

binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: 249

3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
36% identity, 84% coverage: 2:235/278 of query aligns to 1:247/296 of 3c52A

query
sites
3c52A

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A

S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A
x
C

E
|
E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D
|
D

K

A

S
|
S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E
|
E

I

L

G
|
G

H

R

V

L

G

-

S

M

G

G

V

I

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

V

Y

L

T

V

E

N

V

P

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

A

-

F

-
x
K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G
|
G

G

A

S
|
S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S
x
A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N
|
N

I

T

F
x
D

T
|
T

D

D

F

L

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H169 (= H180), A215 (≠ S212), N242 (= N230)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding phosphoglycolohydroxamic acid: D82 (= D82), H83 (= H83), H169 (= H180), K173 (vs. gap), H199 (= H208), G200 (= G209), S202 (= S211), N242 (= N230), D244 (≠ F232), T245 (= T233)
binding zinc ion: H83 (= H83), H169 (= H180), H199 (= H208)

5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
36% identity, 84% coverage: 2:235/278 of query aligns to 1:243/292 of 5ucpA

query
sites
5ucpA

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A
x
S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A

C

E

E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E
|
E

I

L

G

G

H

R

V

V

G

-

S

-

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

-

Y

L

T

V

E

N

V

P

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

A

-

F

-
x
K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G
|
G

G

A

S
|
S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S

A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N
|
N

I

T

F
x
D

T
|
T

D

D

F

L

binding 1,6-di-O-phosphono-D-fructose: S49 (≠ A50), D82 (= D82), H83 (= H83), H165 (= H180), K169 (vs. gap), G196 (= G209), S198 (= S211), N238 (= N230), D240 (≠ F232), T241 (= T233)
binding zinc ion: H83 (= H83), H83 (= H83), H83 (= H83), E134 (= E134), H165 (= H180), H165 (= H180), H165 (= H180), H195 (= H208), H195 (= H208)

Sites not aligning to the query:

binding 1,6-di-O-phosphono-D-fructose: 244

5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
37% identity, 84% coverage: 2:235/278 of query aligns to 1:242/291 of 5uckA

query
sites
5uckA

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A
x
S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A

C

E

E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E
|
E

I

L

G

G

H

R

V

L

G

-

S

-

G

-

V

V

N

N

Y

P

E

K

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

-

Y

-

T

-

E

-

V

-

S

-

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

A

-

F

-

K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G

G

G

A

S

S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S

A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N

N

I

T

F
x
D

T

T

D

D

F

L

binding glyceraldehyde-3-phosphate: S49 (≠ A50), D82 (= D82), H83 (= H83), H164 (= H180), D239 (≠ F232)
binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H164 (= H180), H194 (= H208), H194 (= H208)

Sites not aligning to the query:

binding glyceraldehyde-3-phosphate: 243

5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
36% identity, 84% coverage: 2:235/278 of query aligns to 1:244/293 of 5ud4A

query
sites
5ud4A

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A
x
S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A

C

E

E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E
|
E

I

L

G

G

H

R

V

L

G

-

S

-

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

V

Y

L

T

V

E

N

V

P

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
x
Q

G
|
G

A

A

-

F

-
x
K

Y

F

K

K

G

G

E

E

P

P

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G
|
G

G

A

S
|
S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S

A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N
|
N

I

T

F
x
D

T
|
T

D

D

F

L

binding 1,6-di-O-phosphono-D-tagatose: S49 (≠ A50), D82 (= D82), Q166 (≠ H180), G167 (= G181), K170 (vs. gap), G197 (= G209), S199 (= S211), N239 (= N230), D241 (≠ F232), T242 (= T233)
binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H196 (= H208), H196 (= H208)

Sites not aligning to the query:

binding 1,6-di-O-phosphono-D-tagatose: 245

6ofuB X-ray crystal structure of the ydji aldolase from escherichia coli k12 (see paper)
34% identity, 91% coverage: 13:264/278 of query aligns to 12:253/264 of 6ofuB

query
sites
6ofuB

A

A

R

T

E

N

E

K

Q

H

Y

Y

A

A

I

I

P

A

A

H

C

F

N

N

F

V

F

W

D

N

L

A

D

E

S

M

A

L

R

M

S

G

Y

V

V

I

A

D

V

A

A

A

E

E

R

E

E

A

N

K

K

S

P

P

L

V

I

I

L

I

A

S

L

F

A

G

E

T

A

G

H

F

L

V

D

G

M

N

I

T

S

S

L

F

E

E

E

D

G

F

A

S

L

H

I

M

G

M

K

V

Y

S

L

M

A

A

E

Q

K

K

A

A

T

T

V

V

P

P

V

V

V

I

L

T

H

H

L

W

D

D

H
|
H

G

G

Q

R

T

S

L

M

S

E

V

I

I

I

E

H

R

N

A

A

I

W

D

T

L

H

D

G

F

M

T

N

S

S

V

L

M

M

I

R

D

D

K

A

S

S

E

A

S

F

T

D

F

F

A

E

E

E

N

N

V

I

A

R

L

L

T

T

K

K

K

E

V

A

V

V

A

D

M

F

A

F

K

H

G

P

K

L

N

G

V

I

A

P

V

V

E

E

A

A

E
|
E

I

L

G

G

H

Y

V

H

G

-

S

-

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

-

Y

Y

T

T

E

D

V

P

S

D

D

Q

A

A

V

A

T

E

F

F

V

V

E

E

E

R

T

T

Q

G

V

C

D

D

S

S

L

L

A

A

I

V

S

A

I

I

G

G

T

N

A

Q

H
|
H

G

G

A

V

Y

Y

K

T

G

S

E

E

P

P

K

Q

I

L

N

N

F

F

D

E

R

V

L

V

I

K

D

R

I

V

A

R

K

D

Q

A

V

V

D

S

I

V

P

P

L

L

V

V

L

L

H
|
H

G

G

G

A

S

S

S

G

S

I

G

S

D

D

E

A

N

D

L

I

K

K

R

T

C

A

A

I

V

S

T

L

G

G

I

I

E

A

K

K

I

I

N

N

I

I

F

H

T

T

D

E

F

L

I

C

N

Q

S

A

A

A

F

M

E

V

A

A

I

V

T

K

S

E

E

N

Q

Q

P

D

T

Q

D

P

Y

F

L

L

Q

H

V

L

-

E

-

R

-

E

-

V

-

R

K

K

A

A

V

V

A

K

N

E

Q

R

A

A

I

L

E

E

K

K

binding zinc ion: H82 (= H83), E133 (= E134), H164 (= H180), H192 (= H208)

A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
39% identity, 76% coverage: 1:212/278 of query aligns to 1:214/323 of A8B2U2

query
sites
A8B2U2

M

M

V

P

L

L

V

C

N

T

S

L

K

R

E

Q

L

M

F

L

K

G

K

E

A

A

R

R

E

K

E

H

Q

K

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

V

F

N

D

N

L

M

D

E

S

Q

A

I

R

Q

S

G

Y

I

V

M

A

K

V

A

A

V

E

V

R

Q

E

L

N

K

K

S

P

P

L

V

I

I

L

L

A

Q

L

C

A
x
S

E

R

A

G

H

A

L

L

-

K

-

Y

-

S

D

D

M

M

I

I

S

Y

L

L

E

K

G

-

A

-

L

L

I

C

G

E

K

A

Y

A

L

L

A

E

E

K

K

H

A

P

T

D

V

I

P

P

V

I

V

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

Q

D

T

T

L

L

S

E

V

S

I

V

E

K

R

M

A

A

I

I

D

D

L

L

D

G

F

F

T

S

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

P

F

F

A

D

E

E

N

N

V

V

A

R

L

I

T

T

K

K

K

E

V

V

A

A

M

Y

A

A

K

H

G

A

K

R

N

G

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G

G

H

T

V

L

G

G

S

-

G

G

V

I

N

-

Y

-

E

-

N

E

H

E

E

D

V

V

K

Q

D

N

S

T

I

V

-

Q

Y

L

T

T

E

E

V

P

S

Q

D

D

A

A

V

K

T

K

F

F

V

V

E

E

E

L

T

T

Q

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

A

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

-

F

-

K

-

S

-

E

G

S

E

D

P

I

K

R

I

L

N

A

F

I

D

D

R

R

L

V

I

K

D

T

I

I

A

S

K

D

Q

L

V

T

D

G

I

I

P

P

L

L

V

V

L

M

H
|
H

G
|
G

G

S

S
|
S

S

S

S50 (≠ A50) binding beta-D-fructose 1,6-bisphosphate
D83 (= D82) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
H84 (= H83) binding Zn(2+)
H178 (= H180) binding beta-D-fructose 1,6-bisphosphate; binding Zn(2+)
G179 (= G181) binding beta-D-fructose 1,6-bisphosphate
K182 (= K184) binding beta-D-fructose 1,6-bisphosphate
H210 (= H208) binding Zn(2+)
G211 (= G209) binding beta-D-fructose 1,6-bisphosphate
S213 (= S211) binding beta-D-fructose 1,6-bisphosphate

Sites not aligning to the query:

253 binding beta-D-fructose 1,6-bisphosphate
255 binding beta-D-fructose 1,6-bisphosphate; D→A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
256 binding beta-D-fructose 1,6-bisphosphate
259 binding beta-D-fructose 1,6-bisphosphate; R→A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
278 D→A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
280 binding beta-D-fructose 1,6-bisphosphate

4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
34% identity, 98% coverage: 3:275/278 of query aligns to 2:273/279 of 4to8A

query
sites
4to8A

L

L

V

V

N

S

S

M

K

K

E

E

L

M

F

L

K

I

K

D

A

A

R

K

E

E

E

N

Q

G

Y

Y

A

A

I

V

P

G

A

Q

C

Y

N

N

F

I

F

N

D

N

L

L

D

E

S

F

A

T

R

Q

S

A

Y

I

V

L

A

E

V

A

A

S

E

Q

R

E

E

E

N

N

K

A

P

P

L

V

I

I

L

L

A

G

L

V

A

S

E

E

A

G

-

A

-

R

-

Y

-

M

-

S

-

G

H

F

L

Y

D

T

M

I

I

V

S

K

L

M

E

V

E

E

G

G

A

L

L

M

I

H

G

D

K

L

Y

N

L

I

A

-

E

-

K

-

A

-

T

T

V

I

P

P

V

V

V

A

L

I

H

H

L

L

D
|
D

H
|
H

G

G

Q

S

T

S

L

F

S

E

V

K

I

C

E

K

R

E

A

A

I

I

D

D

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

S

T

P

F

F

A

E

E

E

N

N

V

V

A

A

L

T

T

T

K

K

V

V

A

E

M

Y

A

A

K

H

G

E

K

K

N

G

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G

G

H

T

V

V

G

G

S

G

G

-

V

-

N

-

Y

-

E

-

N

D

H

D

E

V

V

V

K

A

D

D

S

G

-

I

I

I

Y

Y

T

A

E

D

V

P

S

K

D

E

A

C

V

Q

T

E

F

L

V

V

E

E

E

K

T

T

Q

G

V

I

D

D

S

A

L

L

A

A

I

P

S

A

I

L

G

G

T

S

A

V

H
|
H

G

G

A

-

Y

-

K

-

G

-

E

-

P

P

K

K

I

L

N

G

F

F

D

K

R

E

L

M

I

E

D

E

I

I

A

G

K

L

Q

S

V

T

D

G

I

L

P

P

L

L

V

V

L

L

H
|
H

G

G

S

T

S

G

S

I

G

P

D

T

E

K

N

D

L

I

K

Q

R

K

C

A

A

I

V

P

T

F

G

G

I

T

E

A

K

K

I

I

N
|
N

I

V

F

N

T

T

-

E

-

N

-

Q

-

I

-

A

-

S

-

A

-

K

-

A

-

V

-

R

D

D

F

V

I

L

N

N

S

N

A

D

F

K

E

E

A

V

I

Y

T

-

S

-

E

-

Q

D

P

P

T

R

D

K

Y

Y

L

L

Q

-

V

-

K

-

A

G

V

P

A

A

N

R

Q

E

A

A

I

I

E

K

K

E

T

T

L

V

S

K

H

G

Y

K

Y

I

Q

K

V

E

F

F

E

G

T

T

active site: D84 (= D82), H85 (= H83), H178 (= H180), H201 (= H208), N223 (= N230)
binding zinc ion: H85 (= H83), H201 (= H208)

3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
39% identity, 76% coverage: 3:212/278 of query aligns to 2:210/319 of 3ohiA

query
sites
3ohiA

L

L

V

C

N

T

S

L

K

R

E

Q

L

M

F

L

K

G

K

E

A

A

R

R

E

K

E

H

Q

K

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

V

F

N

D

N

L

M

D

E

S

Q

A

I

R

Q

S

G

Y

I

V

M

A

K

V

A

A

V

E

V

R

Q

E

L

N

K

K

S

P

P

L

V

I

I

L

L

A

Q

L

C

A
x
S

E

R

A

G

H

A

L

L

-

K

-

Y

-

S

D

D

M

M

I

I

S

Y

L

L

E

K

G

-

A

-

L

L

I

C

G

E

K

A

Y

A

L

L

A

E

E

K

K

H

A

P

T

D

V

I

P

P

V

I

V

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

Q

D

T

T

L

L

S

E

V

S

I

V

E

K

R

M

A

A

I

I

D

D

L

L

D

G

F

F

T

S

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

P

F

F

A

D

E

E

N

N

V

V

A

R

L

I

T

T

K

K

K

E

V

V

A

A

M

Y

A

A

K

H

G

A

K

R

N

S

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G

G

H

T

V

L

G

G

S

-

G

-

V

-

N

G

Y

I

E

E

N

Q

H

N

E

T

V

V

K

Q

D

-

S

-

I

-

Y

L

T

T

E

E

V

P

S

Q

D

D

A

A

V

K

T

K

F

F

V

V

E

E

E

L

T

T

Q

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

A

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

-

F

-

K

-

S

-

E

G

S

E

D

P

I

K

R

I

L

N

A

F

I

D

D

R

R

L

V

I

K

D

T

I

I

A

S

K

D

Q

L

V

T

D

G

I

I

P

P

L

L

V

V

L

M

H
|
H

G
|
G

G

S

S
|
S

S

S

binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (≠ A50), D82 (= D82), H83 (= H83), H174 (= H180), G175 (= G181), K178 (= K184), G207 (= G209), S209 (= S211)
binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H208)

Sites not aligning to the query:

binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: 249, 251, 252, 255

3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
39% identity, 76% coverage: 3:212/278 of query aligns to 2:210/319 of 3gayA

query
sites
3gayA

L

L

V

C

N

T

S

L

K

R

E

Q

L

M

F

L

K

G

K

E

A

A

R

R

E

K

E

H

Q

K

Y

Y

A

G

I

V

P

G

A

A

C

F

N
|
N

F

V

F

N

D

N

L

M

D

E

S

Q

A

I

R

Q

S

G

Y

I

V

M

A

K

V

A

A

V

E

V

R

Q

E

L

N

K

K

S

P

P

L

V

I

I

L

L

A

Q

L

C

A
x
S

E

R

A

G

H

A

L

L

-

K

-

Y

-

S

D

D

M

M

I

I

S

Y

L

L

E

K

G

-

A

-

L

L

I

C

G

E

K

A

Y

A

L

L

A

E

E

K

K

H

A

P

T

D

V

I

P

P

V

I

V

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

Q

D

T

T

L

L

S

E

V

S

I

V

E

K

R

M

A

A

I

I

D

D

L

L

D

G

F

F

T

S

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

P

F

F

A

D

E

E

N

N

V

V

A

R

L

I

T

T

K

K

K

E

V

V

A

A

M

Y

A

A

K

H

G

A

K

R

N

G

V

V

A

S

V

V

E

E

A

A

E

E

I

L

G

G

H

T

V

L

G

G

S

-

G

-

V

-

N

G

Y

I

E

E

N

Q

H

N

E

T

V

V

K

Q

D

-

S

-

I

-

Y

L

T

T

E

E

V

P

S

Q

D

D

A

A

V

K

T

K

F

F

V

V

E

E

E

L

T

T

Q

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

A

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

-

F

-

K

-

S

-

E

G

S

E

D

P

I

K

R

I

L

N

A

F

I

D

D

R

R

L

V

I

K

D

T

I

I

A

S

K

D

Q

L

V

T

D

G

I

I

P

P

L

L

V

V

L

M

H
|
H

G
|
G

G

S

S
|
S

S

S

binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), S49 (≠ A50), D82 (= D82), H174 (= H180), G175 (= G181), K178 (= K184), H206 (= H208), G207 (= G209), S209 (= S211)
binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H208)

Sites not aligning to the query:

binding 1,6-di-O-phosphono-D-tagatose: 249, 251, 252, 255

5ud0A Class ii fructose-1,6-bisphosphate aldolase e149a variant of helicobacter pylori with cleavage products (see paper)
35% identity, 84% coverage: 2:235/278 of query aligns to 1:233/282 of 5ud0A

query
sites
5ud0A

V

M

L

L

V

V

N

K

S

G

K

N

E

E

L

I

F

L

K

L

K

K

A

A

R

H

E

K

E

E

Q

G

Y

Y

A

G

I

V

P

G

A

A

C

F

N

N

F

F

F

V

D

N

L

F

D

E

S

M

A

L

R

N

S

A

Y

I

V

F

A

E

V

A

A

G

E

N

R

E

E

E

N

N

K

S

P

P

L

L

I

F

L

I

A

Q

L

A

A

S

E

E

A

G

H

A

L

I

D

K

M

Y

I

M

S

G

L

I

E

D

E

M

G

A

A

V

L

G

I

M

G

V

K

K

Y

I

L

M

A

C

E

E

K

R

-

Y

A

P

T

H

V

I

P

P

V

V

V

A

L

L

H

H

L

L

D

D

H
|
H

G

G

Q

T

T

T

L

F

S

E

V

S

I

C

E

E

R

K

A

A

I

V

D

K

L

A

D

G

F

F

T

T

S

S

V

V

M

M

I

I

D

D

K

A

S

S

E

H

S

H

T

A

F

F

A

E

E

E

N

N

V

L

A

E

L

L

T

T

K

S

K

K

V

V

A

K

M

M

A

A

K

H

G

N

K

A

N

G

V

V

A

S

V

V

E

E

A

A

E
|
E

I

L

G

G

H

L

V

V

G

N

S

P

G

-

V

-

N

-

Y

-

E

-

N

-

H

-

E

-

V

-

K

-

D

-

S

-

I

-

Y

-

T

-

E

-

V

-

S

K

D

E

A

A

V

E

T

Q

F

F

V

V

E

K

E

E

T

S

Q

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

A

S

H
|
H

G

G

A

-

Y

-

K

-

G

-

E

-

P

-

K

K

I

L

N

D

F

F

D

E

R

R

L

L

I

Q

D

E

I

V

A

K

K

R

Q

L

V

T

D

N

I

I

P

P

L

L

V

V

L

L

H
|
H

G

G

G

A

S

S

-

A

-

I

-

P

-

D

-

N

-

V

-

R

-

K

-

S

-

Y

-

L

-

D

S

A

S

G

G

G

D

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

E

E

N

F

L

L

K

Q

R

E

C

S

A

V

V

K

T

G

G

G

I

I

E

N

K

K

I

V

N

N

I

T

F

D

T

T

D

D

F

L

binding zinc ion: H83 (= H83), E134 (= E134), H163 (= H180), H185 (= H208)

Query Sequence

>WP_077275372.1 NCBI__GCF_001998885.1:WP_077275372.1
MVLVNSKELFKKAREEQYAIPACNFFDLDSARSYVAVAERENKPLILALAEAHLDMISLE
EGALIGKYLAEKATVPVVLHLDHGQTLSVIERAIDLDFTSVMIDKSESTFAENVALTKKV
VAMAKGKNVAVEAEIGHVGSGVNYENHEVKDSIYTEVSDAVTFVEETQVDSLAISIGTAH
GAYKGEPKINFDRLIDIAKQVDIPLVLHGGSSSGDENLKRCAVTGIEKINIFTDFINSAF
EAITSEQPTDYLQVKAVANQAIEKTLSHYYQVFETKEV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory