SitesBLAST
Comparing WP_077275495.1 NCBI__GCF_001998885.1:WP_077275495.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 17 hits to proteins with known functional sites (download)
2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
50% identity, 100% coverage: 1:311/312 of query aligns to 2:312/319 of 2f02A
- binding adenosine-5'-triphosphate: N186 (= N186), S224 (= S223), G226 (= G225), I243 (= I242), I246 (= I245), G253 (= G252), S254 (= S253), G255 (= G254), T258 (= T257), M280 (= M279), G283 (= G282), M284 (= M283)
2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
50% identity, 100% coverage: 1:311/312 of query aligns to 6:315/315 of 2jg1C
- active site: G256 (= G252), S257 (= S253), G258 (= G254), D259 (= D255)
- binding phosphoaminophosphonic acid-adenylate ester: S227 (= S223), G229 (= G225), A230 (≠ S226), G232 (= G228), I246 (= I242), I249 (= I245), V251 (= V247), V255 (= V251), G256 (= G252), S257 (= S253), G258 (= G254), D259 (= D255), T261 (= T257), N283 (≠ M279), G286 (= G282), M287 (= M283)
- binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D12), G42 (= G37), K43 (= K38), R93 (= R88), C95 (= C90), L108 (= L104), G140 (= G136), S141 (= S137), D259 (= D255)
2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
50% identity, 100% coverage: 1:311/312 of query aligns to 9:318/318 of 2jg1A
- active site: G259 (= G252), S260 (= S253), G261 (= G254), D262 (= D255)
- binding phosphoaminophosphonic acid-adenylate ester: K46 (= K38), N193 (= N186), S230 (= S223), G232 (= G225), G235 (= G228), I252 (= I245), V254 (= V247), G259 (= G252), S260 (= S253), G261 (= G254), D262 (= D255), T264 (= T257), N286 (≠ M279), G289 (= G282), M290 (= M283)
2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
50% identity, 100% coverage: 1:311/312 of query aligns to 5:314/314 of 2jgvB
- active site: G255 (= G252), S256 (= S253), G257 (= G254), D258 (= D255)
- binding adenosine-5'-diphosphate: S226 (= S223), A229 (≠ S226), I248 (= I245), P253 (= P250), S256 (= S253), G257 (= G254), N282 (≠ M279), G285 (= G282), M286 (= M283)
P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
28% identity, 96% coverage: 2:302/312 of query aligns to 14:312/339 of P9WID3
- K283 (= K273) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
26% identity, 96% coverage: 2:302/312 of query aligns to 4:303/309 of 3uqdB
- active site: G253 (= G252), A254 (≠ S253), G255 (= G254), D256 (= D255)
- binding adenosine-5'-diphosphate: K185 (= K184), N187 (= N186), S224 (= S223), G226 (= G225), P227 (≠ S226), G229 (= G228), S248 (≠ V247), M258 (≠ T257), V280 (≠ M279), G283 (= G282), S284 (≠ M283)
- binding adenosine-5'-triphosphate: Y23 (≠ N21), K27 (≠ V25)
- binding 6-O-phosphono-beta-D-fructofuranose: K27 (≠ V25), R29 (= R27)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D12), G39 (= G37), N43 (= N41), R90 (= R88), R105 (≠ E102), S139 (= S137), G253 (= G252)
3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
26% identity, 96% coverage: 2:302/312 of query aligns to 4:303/309 of 3uqdA
- active site: G253 (= G252), A254 (≠ S253), G255 (= G254), D256 (= D255)
- binding adenosine-5'-triphosphate: K185 (= K184), S224 (= S223), G226 (= G225), P227 (≠ S226), G229 (= G228), T251 (≠ P250), G255 (= G254), M258 (≠ T257), V280 (≠ M279), G283 (= G282), S284 (≠ M283), T287 (≠ A286)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), G38 (= G36), G39 (= G37), N43 (= N41), R90 (= R88), S139 (= S137), D256 (= D255)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (≠ V25), R29 (= R27)
3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
26% identity, 96% coverage: 2:302/312 of query aligns to 4:303/309 of 3n1cA
- active site: G253 (= G252), A254 (≠ S253), G255 (= G254), D256 (= D255)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), K27 (≠ V25), R29 (= R27), G39 (= G37), N43 (= N41), R90 (= R88), G138 (= G136), S139 (= S137), D256 (= D255)
P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
26% identity, 96% coverage: 2:302/312 of query aligns to 4:303/309 of P06999
- K27 (≠ V25) binding ATP; binding ATP
- KPN 185:187 (= KPN 184:186) binding in other chain
- NQK 187:189 (≠ NLD 186:188) binding in other chain
- E190 (= E189) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
- SLGPQG 224:229 (≠ SLGSDG 223:228) binding in other chain
- S248 (≠ V247) binding in other chain
- S250 (≠ N249) binding K(+)
- V252 (= V251) binding K(+)
- V280 (≠ M279) binding in other chain
- S284 (≠ M283) binding in other chain
- A286 (≠ N285) binding K(+)
- N289 (≠ H288) binding K(+)
- G291 (≠ A290) binding K(+)
- R293 (≠ G292) binding K(+)
3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
27% identity, 92% coverage: 2:289/312 of query aligns to 4:290/307 of 3uqeA
- active site: G253 (= G252), A254 (≠ S253), G255 (= G254), D256 (= D255)
- binding adenosine-5'-triphosphate: K185 (= K184), N187 (= N186), S224 (= S223), G226 (= G225), P227 (≠ S226), G229 (= G228), S248 (≠ V247), A254 (≠ S253), G255 (= G254), M258 (≠ T257), V280 (≠ M279), G283 (= G282), S284 (≠ M283), T287 (≠ A286)
- binding pyrophosphate 2-: N187 (= N186), K189 (≠ D188)
3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
27% identity, 90% coverage: 2:282/312 of query aligns to 4:283/304 of 3cqdA
- active site: G253 (= G252), A254 (≠ S253), G255 (= G254), D256 (= D255)
- binding adenosine-5'-triphosphate: Y23 (≠ N21), G26 (≠ Q24), K27 (≠ V25), K185 (= K184), N187 (= N186), N187 (= N186), K189 (≠ D188), S224 (= S223), G226 (= G225), P227 (≠ S226), G229 (= G228), S248 (≠ V247), T251 (≠ P250), A254 (≠ S253), G255 (= G254), M258 (≠ T257), V280 (≠ M279), G283 (= G282)
Sites not aligning to the query:
3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
25% identity, 100% coverage: 1:312/312 of query aligns to 2:309/309 of 3ie7A
- binding adenosine-5'-triphosphate: N188 (= N186), S220 (= S223), G222 (= G225), A223 (≠ S226), G225 (= G228), V242 (≠ I245), G249 (= G252), A250 (≠ S253), G251 (= G254), D252 (= D255), S279 (≠ G282), V283 (≠ A286)
3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
27% identity, 93% coverage: 1:289/312 of query aligns to 2:279/298 of 3julA
2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
29% identity, 60% coverage: 1:187/312 of query aligns to 2:194/320 of 2ajrA
Sites not aligning to the query:
3kzhA Crystal structure of a putative sugar kinase from clostridium perfringens
26% identity, 66% coverage: 85:291/312 of query aligns to 91:290/316 of 3kzhA
3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
24% identity, 80% coverage: 30:280/312 of query aligns to 33:283/312 of 3in1A
- active site: R106 (≠ Q100), G255 (= G252), A256 (≠ S253), G257 (= G254), D258 (= D255)
- binding adenosine-5'-diphosphate: N194 (= N186), K225 (≠ S223), G227 (= G225), G230 (= G228), A244 (≠ T241), T253 (≠ P250), N282 (≠ M279)
Sites not aligning to the query:
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
25% identity, 77% coverage: 36:276/312 of query aligns to 38:273/306 of 4xckA
- active site: A249 (≠ G252), A250 (≠ S253), G251 (= G254), D252 (= D255)
- binding adenosine-5'-diphosphate: T220 (≠ S223), G222 (= G225), S223 (= S226), V242 (≠ I245), T247 (≠ P250), A250 (≠ S253), F254 (≠ T257)
- binding alpha-D-ribofuranose: G39 (= G37), K40 (= K38), N43 (= N41), A95 (= A92), I107 (≠ E102), I109 (≠ L104), E140 (≠ P139), T248 (≠ V251), D252 (= D255)
Sites not aligning to the query:
Query Sequence
>WP_077275495.1 NCBI__GCF_001998885.1:WP_077275495.1
MIVTVTMNPSVDIAYTLPHLNLDQVNRCNQVSKTAGGKGLNVTRVIHQMNQDVVSTGLLG
GALGDFIRESLTKDGIKHNFSDIDGQTRNCLAILHDNGAQTEILEAGPTITAAELKRFEQ
VMANLLPESDVMTLSGSLPKGIPTNYYVRLLELMSKDSIKVVLDTSGQSLIEVLQSSVKP
YAIKPNLDELSDLTNQILTADETALKQALTADIFEGIPLIVVSLGSDGAFVKYTDRFYRV
TIPKITVVNPVGSGDSTVAGIAIGLQKKESIEKVIKRAMALGMSNATHQATGYIDQDSYQ
EFEKQILVQEIK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory