SitesBLAST

Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
70% identity, 98% coverage: 6:260/260 of query aligns to 2:256/256 of Q48436

query
sites
Q48436

Q

K

H

K

V

V

A

A

F
x
L

V
|
V

T
|
T

G
|
G

G
x
A

G
|
G

Q
|
Q

G
|
G

I
|
I

G
|
G

K
|
K

A
|
A

I
|
I

C
x
A

L
|
L

R
|
R

L
|
L

A
x
V

Q
x
K

D
|
D

G
|
G

F
|
F

K
x
A

I
x
V

A
|
A

V
x
I

A
|
A

D
|
D

Y

Y

N

N

F

D

D

A

T

T

A

A

T

K

E

A

V

V

A

A

N

S

E

E

I

I

N

N

N

Q

K

A

E

G

G

G

Q

R

A

A

V

M

A

A

I

V

K

K

V

V

D
|
D

V

V

S

S

D

D

R

R

E

D

A

Q

V

V

F

F

A

A

V

V

E

E

E

Q

A

A

K

R

E

K

K

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

A

A

G

G

L

V

G

A

P

P

Q

S

T

T

P

P

I

I

E

E

E

S

I

I

T

T

Y

P

D

E

T

I

Y

V

R

D

R

K

V

V

F

Y

D

N

I

I

N

N

V

V

G

K

G

G

T

V

I

I

W

W

G

G

M

I

Q

Q

A

A

I

V

K

E

A

A

F

F

K

K

S

K

L

E

G

G

H

H

G

G

K

K

I

I

N

N

A

A

S

C

S

S

Q

Q

A

A

G

G

Q

H

V

V

G

G

N

N

P

P

G

E

L

L

A

A

V

V

Y

Y

G

S

S

S

K
|
K

F

F

A

A

I

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

K

R

D

D

L

L

A

A

N

P

L

L

G

G

I

I

T

T

V

V

N

N

A

G

Y

Y

C

C

P

P

G

G

I

I

V

V

K

K

T

T

P

P

M

M

M

W

M

A

G

E

I

I

A

D

E

R

Q

Q

T

V

A

S

K

E

E

A

A

A

G

G

K

K

P

P

F

L

E

G

W

Y

G

G

L

T

E

A

Q

E

F

F

S

A

Q

K

N

R

I

I

T

T

L

L

K

G

R

R

L

L

S

S

E

E

P

P

E

E

D

D

V

V

A

A

C

C

V

V

S

S

Y

Y

L

L

A

A

G

S

P

P

D

D

S

S

D

D

Y

Y

M

M

T

T

G

G

Q

Q

A

S

L

L

I

L

I

I

D

D

G

G

M

M

T

V

F

F

N

N

6:33 (vs. 10:37, 71% identical) binding NAD(+)
D59 (= D63) binding NAD(+)
K156 (= K160) binding NAD(+)

3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
60% identity, 98% coverage: 6:260/260 of query aligns to 1:255/255 of 3wyeA

query
sites
3wyeA

Q

K

H

K

V

V

A

A

F

L

V

V

T

T

G
|
G

G

A

G

G

Q
|
Q

G
|
G

I
|
I

G

G

K

K

A

A

I

I

C

A

L

L

R

R

L

L

A

V

Q

K

D

D

G

G

F

F

K

A

I

V

A

A

V

I

A

A

D
|
D

Y
|
Y

N

N

F

D

D

A

T

T

A

A

T

K

E

A

V

V

A

A

N

S

E

E

I

I

N

N

N

Q

K

A

E

G

G

G

Q

H

A

A

V

V

A

A

I

V

K

K

V
|
V

D
|
D

V
|
V

S

S

D

D

R

R

E

D

A

Q

V

V

F

F

A

A

V

V

E

E

E

Q

A

A

K

R

E

K

K

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

N
|
N

A
|
A

G

G

L

I

G

A

P

Q

Q

I

T

K

P

P

I

L

E

L

E

E

I

V

T

T

Y

E

D

E

T

D

Y

L

R

K

R

Q

V

I

F

Y

D

S

I

V

N

N

V

V

G

F

G

S

T

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

V

K

E

A

A

F

F

K

K

S

K

L

E

G

G

H

H

G

G

K

K

I

I

N

N

A

A

S

A

S
|
S

Q

I

A

A

G

A

Q

I

V

Q

G

G

N

F

P

P

G

I

L

L

A

S

V

A

Y
|
Y

G

S

S

T

K
|
K

F

F

A

A

I

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

K

R

D

D

L

L

A

A

N

P

L

L

G

G

I

I

T

T

V

V

N

N

A

G

Y

Y

C

C

P
|
P

G
|
G

I

I

V
|
V

K

G

T
|
T

P

G

M
|
M

M
x
W

M

E

G

Q

I

I

A

D

E

A

Q

E

T
x
L

A

S

K

K

E

I

A

N

G

G

K

K

P

P

F

I

E

G

W

E

G

N

L

F

E

K

Q

E

F

Y

S

S

Q

S

N

S

I

I

T

A

L

L

K

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

P

D

D

S

S

D

D

Y

Y

M

M

T

T

G

G

Q

Q

A

S

L

L

I

L

I

I

D

D

G

G

M

M

T

V

F

F

N

N

active site: G12 (= G17), S138 (= S143), Y151 (= Y156), K155 (= K160), L196 (≠ T201)
binding nicotinamide-adenine-dinucleotide: G8 (= G13), Q11 (= Q16), G12 (= G17), I13 (= I18), D32 (= D37), Y33 (= Y38), V57 (= V62), D58 (= D63), V59 (= V64), N85 (= N90), A86 (= A91), S138 (= S143), Y151 (= Y156), K155 (= K160), P181 (= P186), G182 (= G187), V184 (= V189), T186 (= T191), M188 (= M193), W189 (≠ M194)

Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
48% identity, 98% coverage: 7:260/260 of query aligns to 3:258/258 of Q9ZNN8

query
sites
Q9ZNN8

H

K

V

V

A

A

F

M

V

V

T

T

G

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

C

S

L

E

R

K

L

L

A

A

Q

A

D

D

G

G

F

F

K

D

I

I

A

A

V

V

A

A

D
|
D

Y

L

-

P

-

Q

N
x
Q

F

E

D

E

T

Q

A

A

T

A

E

E

V

T

A

I

N

K

E

L

I

I

N

E

N

A

K

A

E

D

G

Q

Q

K

A

A

V

V

A

F

I

V

K

G

V

L

D
|
D

V
|
V

S

T

D

D

R

K

E

A

A

N

V

F

F

D

A

S

A

A

V

I

E

D

E

E

A

A

K

A

E

E

K

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

L

I

G

A

P

Q

Q

I

T

K

P

P

I

L

E

L

E

E

I

V

T

T

Y

E

D

E

T

D

Y

L

R

K

R

Q

V

I

F

Y

D

S

I

V

N

N

V

V

G

F

G

S

T

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

S

K

R

A

K

F

F

K

D

S

E

L

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A

A

S

A

S

S

Q
x
I

A

A

G

A

Q

I

V

Q

G

G

N
x
F

P

P

G

I

L

L

A

S

V

A

Y
|
Y

G

S

S

T

K
|
K

F

F

A

A

I

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

K

Q

D

E

L

L

A

A

N

P

L

K

G

G

I

H

T

T

V

V

N

N

A

A

Y

Y

C

A

P
|
P

G
|
G

I
|
I

V
|
V

K
x
G

T
|
T

P

G

M

M

M

W

M

E

G

Q

I

I

A

D

E

A

Q

E

T

L

A

S

K

K

E

I

A

N

G

G

K

K

P

P

F

I

E

G

W

E

G

N

L

F

E

K

Q

E

F

Y

S

S

Q

S

N

S

I

I

T

A

L

L

K

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

E

D

N

S

S

D

N

Y

Y

M

V

T

T

G

G

Q

Q

A

V

L

M

I

L

I

V

D

D

G

G

M

M

T

L

F

Y

N

N

QGI 12:14 (= QGI 16:18) binding NAD(+)
D33 (= D37) binding NAD(+)
Q37 (≠ N39) binding NAD(+)
DV 61:62 (= DV 63:64) binding NAD(+)
N88 (= N90) binding NAD(+)
I142 (≠ Q144) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
F148 (≠ N150) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
Y154 (= Y156) binding NAD(+)
K158 (= K160) binding NAD(+)
PGIVGT 184:189 (≠ PGIVKT 186:191) binding NAD(+)

3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
48% identity, 98% coverage: 7:260/260 of query aligns to 2:257/257 of 3a28C

query
sites
3a28C

H

K

V

V

A

A

F

M

V

V

T

T

G
|
G

G

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

C

S

L

E

R

K

L

L

A

A

Q

A

D

D

G

G

F

F

K

D

I

I

A

A

V

V

A

A

D
|
D

Y
x
L

-

P

-

Q

N
x
Q

F

E

D

E

T

Q

A

A

T

A

E

E

V

T

A

I

N

K

E

L

I

I

N

E

N

A

K

A

E

D

G

Q

Q

K

A

A

V

V

A

F

I

V

K

G

V
x
L

D
|
D

V
|
V

S

T

D

D

R

K

E

A

A

N

V

F

F

D

A

S

A

A

V

I

E

D

E

E

A

A

K

A

E

E

K

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

L

I

G

A

P

Q

Q

I

T

K

P

P

I

L

E

L

E

E

I

V

T

T

Y

E

D

E

T

D

Y

L

R

K

R

Q

V

I

F

Y

D

S

I

V

N

N

V

V

G

F

G

S

T

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

S

K

R

A

K

F

F

K

D

S

E

L

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A

A

S

A

S
|
S

Q

I

A

A

G

A

Q

I

V

Q

G

G

N

F

P

P

G

I

L

L

A

S

V

A

Y
|
Y

G

S

S

T

K
|
K

F

F

A

A

I

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

K

Q

D

E

L

L

A

A

N

P

L

K

G

G

I

H

T

T

V

V

N

N

A

A

Y

Y

C

A

P
|
P

G

G

I

I

V
|
V

K

G

T
|
T

P

G

M
|
M

M
x
W

M

E

G

Q

I

I

A

D

E

A

Q

E

T
x
L

A

S

K

K

E

I

A

N

G

G

K

K

P

P

F

I

E

G

W

E

G

N

L

F

E

K

Q

E

F

Y

S

S

Q

S

N

S

I

I

T

A

L

L

K

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

Y

F

L

L

A

A

G

S

P

E

D

N

S

S

D

N

Y

Y

M

V

T

T

G

G

Q

Q

A

V

L

M

I

L

I

V

D

D

G

G

M

M

T

L

F

Y

N

N

active site: G12 (= G17), S140 (= S143), Y153 (= Y156), K157 (= K160), L198 (≠ T201)
binding nicotinamide-adenine-dinucleotide: G8 (= G13), Q11 (= Q16), I13 (= I18), D32 (= D37), L33 (≠ Y38), Q36 (≠ N39), L59 (≠ V62), D60 (= D63), V61 (= V64), N87 (= N90), S140 (= S143), Y153 (= Y156), K157 (= K160), P183 (= P186), V186 (= V189), T188 (= T191), M190 (= M193), W191 (≠ M194)

6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
36% identity, 95% coverage: 9:256/260 of query aligns to 9:253/257 of 6pejA

query
sites
6pejA

A

A

F

L

V

I

T

T

G

G

G

S

G

A

Q

R

G
|
G

I

I

G

G

K

R

A

A

I

F

C

A

L

E

R

A

L

Y

A

V

Q

R

D

E

G

G

F

A

K

T

I

V

A

A

V

I

A

A

D

D

Y

I

N

D

F

I

D

E

T

R

A

A

T

R

E

Q

V

A

A

A

N

A

E

E

I

I

N

G

N

P

K

A

E

-

G

-

Q

-

A

A

V

Y

A

A

I

V

K

Q

V

M

D

D

V

V

S

T

D

R

R

Q

E

D

A

S

V

I

F

D

A

A

V

I

E

A

A

T

K

V

E

E

K

H

L

A

G

G

F

L

D

D

V

I

I

L

V

V

N

N

A

A

G

A

L

L

G

F

P

D

Q

L

T

A

P

P

I

I

E

V

E

E

I

I

T

T

Y

R

D

E

T

S

Y

Y

R

E

R

K

V

L

F

F

D

A

I

I

N

N

V

V

G

A

G

G

T

T

I

L

W

F

G

T

M

L

Q

Q

A

A

I

A

K

R

A

Q

F

M

K

I

S

A

L

Q

G

G

H

R

G

G

K

K

I

I

N

N

A

M

S

A

S
|
S

Q
|
Q

A

A

G

G

Q

R

V

R

G

G

N
x
E

P

A

G

L

L

V

A

A

V

I

Y
|
Y

G

C

S

A

S

T

K

K

F

A

A

A

I

V

R

I

G

S

L

L

T

T

Q

Q

T

S

A

A

A

G

K

L

D

D

L

L

A

I

N

K

L

H

G

R

I

I

T

N

V

V

N

N

A

A

Y

I

C

A

P

P

G

G

I

V

V

V

K

D

T

G

P

E

M
x
H

M
x
W

M

D

G

G

I

V

A

D

E

A

Q

L

T
x
F

A

A

K

R

E

Y

A

E

G

N

K

R

P

P

F

R

E

G

W

E

G

K

L

K

E

R

Q

L

F

V

S

G

Q

E

N

A

I

V

T

P

L

F

K

G

R

R

L

M

S

G

E

T

P

A

E

E

D

D

V

L

A

T

A

G

C

M

V

A

S

I

Y

F

L

L

A

A

G

S

P

A

D

E

S

S

D

D

Y

Y

M

I

T

V

G

S

Q

Q

A

T

L

Y

I

N

I

V

D

D

G

G

active site: G17 (= G17), S140 (= S143), Y153 (= Y156)
binding sorbitol: Q141 (= Q144), E147 (≠ N150), H190 (≠ M193), W191 (≠ M194), F198 (≠ T201)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 100% coverage: 1:259/260 of query aligns to 2:244/244 of 4nbuB

query
sites
4nbuB

M

M

V

S

E

R

S

L

K

Q

Q

D

H

K

V

V

A

A

F

I

V

I

T

T

G
|
G

G

A

Q
x
N

G
|
G

I
|
I

G

G

K

L

A

E

I

A

C

A

L

R

R

V

L

F

A

M

Q

K

D

E

G

G

F

A

K

K

I

V

A

V

V

I

A

A

D
|
D

Y
x
F

N

N

F

-

D

E

T

A

A

A

T

G

E

K

V

E

A

A

N

V

E

E

I

A

N

N

N

P

K

G

E

-

G

-

Q

-

A

V

V

V

A

F

I

I

K

R

V
|
V

D
|
D

V
|
V

S

S

D

D

R

R

E

E

A

S

V

V

F

H

A

R

A

L

V

V

E

E

E

N

A

V

K

A

E

E

K

R

L

F

G

G

G

K

F

I

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

L
x
I

G

T

P

R

Q
x
D

T

S

P

M

I

L

E

S

E
x
K

I

M

T

T

Y

V

D

D

T

Q

Y

F

R

Q

V

V

F

I

D

N

I

V

N
|
N

V

L

G

T

G

G

T

V

I

F

W

H

G

C

M

T

Q

Q

A

A

A

V

I

L

K

P

A

Y

F

M

K

A

S

E

L

Q

G

G

H

K

G

-

G

G

K

K

I

I

N

N

A
x
T

S

S

S
|
S

Q

V

A

T

G

G

Q

T

V

Y

G

G

N
|
N

P
x
V

G

G

L
x
Q

A

T

V

N

Y
|
Y

G

A

S

A

K
|
K

F

A

A

G

I

V

R

I

G

G

L

M

T

T

Q

K

T

T

A

W

A

A

K

K

D

E

L

L

A

A

N

R

L

K

G

G

I

I

T

N

V

V

N

N

A

A

Y

V

C

A

P
|
P

G

G

I
x
F

V
x
T

K

E

T
|
T

P

A

M
|
M

M

V

M

A

G

E

I

V

A

P

E

E

Q

K

T

V

A

-

K

-

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L

I

E

E

Q
x
K

F

M

S

K

Q

A

N

Q

I

V

T

P

L

M

K

G

R

R

L

L

S

G

E

K

P

P

E

E

D

D

V

I

A

A

A

N

C

A

V

Y

S

L

Y

F

L

L

A

A

G

S

P

H

D

E

S

S

D

D

Y

Y

M

V

T

N

G

G

Q

H

A

V

L

L

I

H

I

V

D

D

G

G

M

I

T

M

F

M

active site: G18 (= G17), N111 (= N114), S139 (= S143), Q149 (≠ L153), Y152 (= Y156), K156 (= K160)
binding acetoacetyl-coenzyme a: D93 (≠ Q96), K98 (≠ E101), S139 (= S143), N146 (= N150), V147 (≠ P151), Q149 (≠ L153), Y152 (= Y156), F184 (≠ I188), M189 (= M193), K200 (≠ Q215)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ Q16), G18 (= G17), I19 (= I18), D38 (= D37), F39 (≠ Y38), V59 (= V62), D60 (= D63), V61 (= V64), N87 (= N90), A88 (= A91), G89 (= G92), I90 (≠ L93), T137 (≠ A141), S139 (= S143), Y152 (= Y156), K156 (= K160), P182 (= P186), F184 (≠ I188), T185 (≠ V189), T187 (= T191), M189 (= M193)

3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
38% identity, 97% coverage: 9:260/260 of query aligns to 10:258/258 of 3ak4A

query
sites
3ak4A

A

A

F

I

V

V

T

T

G

G

G

S

Q
x
K

G
|
G

I
|
I

G

G

K

A

A

A

I

I

C

A

L

R

R

A

L

L

A

D

Q

K

D

A

G

G

F

A

K

T

I

V

A

A

V

I

A

A

D
|
D

Y
x
L

N

D

F

V

D

M

T

A

A

A

T

Q

E

A

V

V

A

V

N

A

E

G

I

L

N

E

N

N

K

G

E

-

G

-

Q

-

A

G

V

F

A

A

I

V

K

E

V
|
V

D
|
D

V
|
V

S

T

D

K

R

R

E

A

A

S

V

V

F

D

A

A

V

M

E

Q

E

K

A

A

K

I

E

D

K

A

L

L

G

G

F

F

D

D

V

L

I

L

V

C

N

A

N
|
N

A
|
A

G
|
G

L

V

G

S

P

T

Q

M

T

R

P

P

I

A

E

V

E

D

I

I

T

T

Y

D

D

E

T

E

Y

W

R

D

R

F

V

N

F

F

D

D

I

V

N

N

V

A

G

R

G

G

T

V

I

F

W

L

G

A

M

N

Q

Q

A

I

A

A

I

C

K

R

A

H

F

F

K

L

S

A

L

S

G

N

H

T

G

K

G

G

K

V

I

I

I

V

N

N

A
x
T

S

A

S
|
S

Q

L

A

A

G

A

Q

K

V

V

G

G

N

A

P

P

G

L

L
|
L

A

A

V

H

Y
|
Y

G

S

S

A

S

S

K
|
K

F

F

A

A

I

V

R

F

G

G

L

W

T

T

Q

Q

T

A

A

L

A

A

K

R

D

E

L

M

A

A

N

P

L

K

G

N

I

I

T

R

V

V

N

N

A

C

Y

V

C

C

P

P

G
|
G

I

F

V
|
V

K

K

T
|
T

P

A

M
|
M

M

Q

M

E

G

R

I

E

A

I

E

I

Q

W

T
x
E

A

A

K

E

E

L

A

R

G

G

K

M

P

T

F

P

E

E

W

A

G

V

L

R

E

A

Q

E

F

Y

S

V

Q

S

N

L

I

T

T

P

L

L

K

G

R

R

L

I

S

E

E

E

P

P

E

E

D

D

V

V

A

A

A

D

C

V

V

V

S

V

Y

F

L

L

A

A

G

S

P

D

D

A

S

A

D

R

Y

F

M

M

T

T

G

G

Q

Q

A

G

L

I

I

N

I

V

D

T

G

G

M

V

T

R

F

M

N

D

active site: G18 (= G17), S141 (= S143), L151 (= L153), Y154 (= Y156), K158 (= K160), E199 (≠ T201)
binding nicotinamide-adenine-dinucleotide: K17 (≠ Q16), G18 (= G17), I19 (= I18), D38 (= D37), L39 (≠ Y38), V60 (= V62), D61 (= D63), V62 (= V64), N88 (= N90), A89 (= A91), G90 (= G92), T139 (≠ A141), S141 (= S143), Y154 (= Y156), K158 (= K160), G185 (= G187), V187 (= V189), T189 (= T191), M191 (= M193)

6j7uA Crystal structure of blue fluorescent protein from metagenomic library in complex with NADPH (see paper)
40% identity, 96% coverage: 8:256/260 of query aligns to 7:244/247 of 6j7uA

query
sites
6j7uA

V

V

A

A

F

F

V

V

T

T

G
|
G

G

G

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

A

A

A

I

I

C

V

L

R

R

R

L

L

A

A

Q

A

D

D

G

G

F

A

K

D

I

I

A

A

-

F

-

T

-
x
Y

V
|
V

A
x
S

D

A

Y

S

N
x
S

F

K

D

N

T

V

A

A

T

T

E

A

V

L

A

V

N

Q

E

E

I

L

N

E

N

A

K

K

E

G

G

R

Q

R

A

A

V

R

A

A

I

I

K

Q

V

A

D
|
D

V
x
S

S

A

D

D

R

P

E

A

A

Q

V

V

F

R

A

Q

A

A

V

V

E

E

E

Q

A

A

K

I

E

V

K

Q

L

L

G

G

G

P

F

V

D

D

V

V

I

L

V

V

N

N

N
|
N

A
|
A

G
|
G

L

I

G

F

P

L

Q

A

T

G

P

P

I

L

E

G

E

E

I

V

T

T

Y

L

D

D

T

D

Y

Y

R

E

R

R

V

T

F

M

D

N

I
|
I

N

N

V

V

G

R

G

A

T

P

I

F

W

V

G

A

M

I

Q

Q

A

A

A

-

I

-

K

-

A

A

F

Q

K

A

S

S

L

M

G

P

H

D

G

G

K

R

I

I

N

N

-

I

A
x
G

S
|
S

S

C

Q

L

A

A

G

E

Q

R

V

A

G

G

N

R

P

A

G

G

L

V

A

T

V

L

Y
|
Y

G

A

S

A

S

S

K
|
K

F

S

A

A

I

L

R

L

G

G

L

M

T

T

Q

R

T

G

A

L

A

A

K

R

D

D

L

L

A

G

N

A

L

R

G

G

I

I

T

T

V

A

N

N

A

V

Y

V

C

H

P
|
P

G

G

I

P

V

I

K

D

T
|
T

P

D

M
|
M

M
x
N

M

P

G

A

I

D

A

G

E

E

Q

R

T

S

A

G

K

-

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L

-

E

-

Q

E

F

L

S

V

Q

A

N

V

I

L

T

S

L

L

K

P

R

H

L

Y

S

G

E

E

P

V

E

R

D

D

V

I

A

A

A

G

C

M

V

V

S

A

Y

F

L

L

A

A

G

G

P

P

D

D

S

G

D

R

Y

Y

M

V

T

T

G

G

Q

A

A

S

L

L

I

A

I

V

D

D

G

G

active site: G16 (= G17), S142 (= S142), Y156 (= Y156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ G15), R15 (≠ Q16), I17 (= I18), Y36 (vs. gap), V37 (= V35), S38 (≠ A36), S41 (≠ N39), D65 (= D63), S66 (≠ V64), N92 (= N90), A93 (= A91), G94 (= G92), I115 (= I113), G141 (≠ A141), S142 (= S142), Y156 (= Y156), K160 (= K160), P186 (= P186), T191 (= T191), M193 (= M193), N194 (≠ M194)

6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
35% identity, 97% coverage: 8:258/260 of query aligns to 8:258/260 of 6zzqA

query
sites
6zzqA

V

V

A

A

F

F

V

I

T

T

G
|
G

G

S

G

A

Q
x
S

G
|
G

I
|
I

G

G

K

L

A

E

I

I

C

A

L

K

R

K

L

F

A

A

Q

Q

D

E

G

G

F

A

K

K

I

V

A

V

V

I

A

S

D
|
D

Y
x
M

N

N

F

A

D

E

T

K

A

C

T

Q

E

E

V

T

A

A

N

N

E

S

I

L

N

K

N

E

K

Q

E

G

G

F

Q

D

A

A

V

L

A

S

I

A

K

P

V

C

D
|
D

V
|
V

S

T

D

D

R

E

E

D

A

A

V

Y

F

K

A

Q

A

A

V

I

E

E

E

L

A

T

K

Q

E

K

K

T

L

F

G

G

G

T

F

V

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

L

F

G
x
Q

P

H

Q

V

T

A

P

P

I

I

E

E

I

F

T

P

Y

T

D

A

T

V

Y

F

R

Q

R

K

V

L

F

V

D

Q

I

V

N

M

V

L

G

T

G

G

T

A

I

F

W

I

G

G

M

I

Q

K

A

H

A

V

I

L

K

P

A

I

F

M

K

K

S

A

L

Q

G

K

H

Y

G

-

G

G

K

R

I

I

N

N

A
x
M

S
x
A

S
|
S

Q

I

A

N

G

G

Q

L

V

I

G

G

N

F

P

A

G

G

L
x
K

A

A

V

G

Y
|
Y

G

N

S

S

S

A

K
|
K

F

H

A

G

I

V

R

I

G

G

L

L

T

T

Q

K

T

V

A

A

K

L

D

E

L

C

A

A

N

R

L

D

G

G

I

I

T

T

V

V

N

N

A

A

Y

L

C

C

P

P

G

G

I
x
Y

V
|
V

K

D

T
|
T

P

P

M

L

M

V

M

R

G

G

I
x
Q

A

I

E

A

Q

D

T

L

A

A

K

K

E

T

A

R

G

N

K

V

P

S

F

L

E

D

W

S

G

A

L

L

E

E

Q

D

-

V

F

I

S

L

Q

A

N

M

I

V

T

P

L

Q

K

K

R

R

L

L

S

L

E

S

P

V

E

E

D

E

V

I

A

A

A

D

C

Y

V

A

S

I

Y

F

L

L

A

A

G

S

P

S

D

K

S

A

D

G

Y

G

M

V

T

T

G

G

Q

Q

A

A

L

V

I

V

I

M

D

D

G

G

M

Y

T

T

active site: G17 (= G17), S142 (= S143), Y155 (= Y156)
binding acetoacetic acid: Q94 (≠ G94), S142 (= S143), K152 (≠ L153), Y155 (= Y156), Q196 (≠ I197)
binding nicotinamide-adenine-dinucleotide: G13 (= G13), S16 (≠ Q16), G17 (= G17), I18 (= I18), D37 (= D37), M38 (≠ Y38), D63 (= D63), V64 (= V64), N90 (= N90), A91 (= A91), G92 (= G92), M140 (≠ A141), A141 (≠ S142), S142 (= S143), Y155 (= Y156), K159 (= K160), Y187 (≠ I188), V188 (= V189), T190 (= T191)

6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
35% identity, 97% coverage: 8:258/260 of query aligns to 9:259/261 of 6zzsD

query
sites
6zzsD

V

V

A

A

F

F

V

I

T

T

G
|
G

G

S

G

A

Q
x
S

G
|
G

I
|
I

G

G

K

L

A

E

I

I

C

A

L

K

R

K

L

F

A

A

Q

Q

D

E

G

G

F

A

K

K

I

V

A

V

V

I

A

S

D
|
D

Y
x
M

N

N

F

A

D

E

T

K

A

C

T

Q

E

E

V

T

A

A

N

N

E

S

I

L

N

K

N

E

K

Q

E

G

G

F

Q

D

A

A

V

L

A

S

I

A

K

P

V

C

D
|
D

V
|
V

S

T

D

D

R

E

E

D

A

A

V

Y

F

K

A

Q

A

A

V

I

E

E

E

L

A

T

K

Q

E

K

K

T

L

F

G

G

G

T

F

V

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

L

F

G
x
Q

P

H

Q

V

T

A

P

P

I

I

E

E

I

F

T

P

Y

T

D

A

T

V

Y

F

R

Q

R

K

V

L

F

V

D

Q

I

V

N

M

V

L

G

T

G

G

T

A

I

F

W

I

G

G

M

I

Q

K

A

H

A

V

I

L

K

P

A

I

F

M

K

K

S

A

L

Q

G

K

H

Y

G

-

G

G

K

R

I

I

N

N

A
x
M

S
x
A

S
|
S

Q

I

A
x
N

G

G

Q

L

V

I

G

G

N

F

P

A

G

G

L
x
K

A

A

V

G

Y
|
Y

G

N

S

S

S

A

K
|
K

F

H

A

G

I

V

R

I

G

G

L

L

T

T

Q

K

T

V

A

A

K

L

D

E

L

C

A

A

N

R

L

D

G

G

I

I

T

T

V

V

N

N

A

A

Y

L

C

C

P
|
P

G
|
G

I

Y

V
|
V

K

D

T
|
T

P

P

M
x
L

M

V

M

R

G

G

I
x
Q

A

I

E

A

Q

D

T

L

A

A

K

K

E

T

A

R

G

N

K

V

P

S

F

L

E

D

W

S

G

A

L

L

E

E

Q

D

-

V

F

I

S

L

Q

A

N

M

I

V

T

P

L

Q

K

K

R

R

L

L

S

L

E

S

P

V

E

E

D

E

V

I

A

A

A

D

C

Y

V

A

S

I

Y

F

L

L

A

A

G

S

P

S

D

K

S

A

D

G

Y

G

M

V

T

T

G

G

Q

Q

A

A

L

V

I

V

I

M

D

D

G

G

M

Y

T

T

active site: G18 (= G17), S143 (= S143), Y156 (= Y156)
binding nicotinamide-adenine-dinucleotide: G14 (= G13), S17 (≠ Q16), I19 (= I18), D38 (= D37), M39 (≠ Y38), D64 (= D63), V65 (= V64), N91 (= N90), A92 (= A91), G93 (= G92), M141 (≠ A141), A142 (≠ S142), S143 (= S143), Y156 (= Y156), K160 (= K160), P186 (= P186), G187 (= G187), V189 (= V189), T191 (= T191), L193 (≠ M193)
binding 3-oxidanylidenepentanoic acid: Q95 (≠ G94), S143 (= S143), N145 (≠ A145), K153 (≠ L153), Y156 (= Y156), Q197 (≠ I197)

8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
37% identity, 99% coverage: 2:259/260 of query aligns to 1:250/251 of 8cxaA

query
sites
8cxaA

V

V

E

R

S

L

K

A

Q

N

H

R

V

V

A

A

F

I

V

I

T

T

G
|
G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

I

I

Y

C

A

L

E

R

R

L

F

A

A

Q

E

D

E

G

G

F

A

K

A

I

V

A

V

V

V

A

A

D
|
D

Y

I

N

N

F

E

D

P

T

K

A

A

T

T

E

E

V

V

A

A

N

S

E

S

I

I

N

T

N

S

K

L

E

G

G

G

Q

R

A

A

V

I

A

A

I

A

K

A

V

V

D

D

V
|
V

S

S

D

D

R

V

E

E

A

S

V

V

F

N

A

R

A

M

V

V

E

D

E

S

A

A

K

V

E

E

K

A

L

F

G

G

G

T

F

V

D

D

V

I

I

L

V

V

N

N

N
|
N

A

A

G
x
A

L

I

G

F

P
x
S

-

T

-

L

-

K

Q

M

T

K

P

P

I

F

E

E

I

I

T

S

Y

G

D

D

T

E

Y

W

R

D

R

K

V

L

F

F

D

A

I

V

N

N

V

A

G

K

G

G

T

T

I

F

W

L

G

C

M

C

Q

Q

A

A

A

V

I

S

K

P

A

V

F

M

K

R

S

K

L

-

G

N

H

Q

G

Y

G

G

K

R

I

I

N

N

A
x
I

S
|
S

Q

S

A

V

G

V

Q

V

V

T

G

G

N

R

P

P

G

N

L

Y

A

A

V

H

Y
|
Y

G

I

S

A

S

S

K
|
K

F

G

A

A

I

V

R

W

G

A

L

L

T

T

Q

H

T

A

A

L

A

A

K

T

D

E

L

M

A

G

N

T

L

D

G

G

I

I

T

T

V

V

N

N

A

T

Y

V

C

S

P
|
P

-
x
H

G

G

I
|
I

V

I

K

T

T

E

P

-

M

-

G

-

I
|
I

A

P

E

R

Q

E

T

T

A

I

K

S

E

E

A

E

G

G

K

-

P

-

F

W

E

R

W

R

G

N

L

L

E

E

Q

E

F

Q

S

A

Q

-

N

-

I

-

T

-

L

L

K

K

R

R

L

K

S

G

E

D

P

A

E

S

D

D

V

L

A

V

A

G

C

I

V

L

S

L

Y

Y

L

L

A

A

G

S

P

D

D

E

S

S

D

K

Y

F

M

M

T

T

G

G

Q

Q

A

T

L

V

I

A

I

L

D

D

G

A

G

G

M

L

T

R

F

F

binding nicotinamide-adenine-dinucleotide: G12 (= G13), Q15 (= Q16), G16 (= G17), I17 (= I18), D36 (= D37), V63 (= V64), N89 (= N90), A91 (≠ G92), S94 (≠ P95), I142 (≠ A141), S143 (= S142), S144 (= S143), Y157 (= Y156), K161 (= K160), P187 (= P186), H188 (vs. gap), I190 (= I188), I194 (= I197)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
35% identity, 96% coverage: 9:257/260 of query aligns to 7:244/246 of 3osuA

query
sites
3osuA

A

A

F

L

V

V

T

T

G

G

G

A

G

S

Q

R

G
|
G

I

I

G

G

K

R

A

S

I

I

C

A

L

L

R

Q

L

L

A

A

Q

E

D

E

G

G

F

Y

K

N

I

V

A

A

V

V

A

-

D

N

Y

Y

-

A

-

G

N

S

F

K

D

E

T

K

A

A

T

E

E

A

V

V

A

V

N

E

E

E

I

I

N

K

N

A

K

K

E

G

G

V

Q

D

A

S

V

F

A

A

I

I

K

Q

V

A

D

N

V

V

S

A

D

D

R

A

E

D

A

E

V

V

F

K

A

A

A

M

V

I

E

K

E

E

A

V

K

V

E

S

K

Q

L

F

G

G

G

S

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

L

I

G

T

P

R

Q

D

T

N

P

L

I

L

E

M

E

R

I

M

T

K

Y

E

D

Q

T

E

Y

W

R

D

V

V

F

I

D

D

I

T

N

N

V

L

G

K

G

G

T

V

I

F

W

N

G

C

M

I

Q

Q

A

K

A

A

I

T

K

P

A

Q

F

M

K

L

S

R

L

-

G

Q

H

R

G

S

G

G

K

A

I

I

N

N

A

L

S

S

S
|
S

Q

V

A

V

G

G

Q

A

V

V

G

G

N

N

P

P

G

G

L
x
Q

A

A

V

N

Y
|
Y

G

V

S

A

S

T

K
|
K

F

A

A

G

I

V

R

I

G

G

L

L

T

T

Q

K

T

S

A

A

K

R

D

E

L

L

A

A

N

S

L

R

G

G

I

I

T

T

V

V

N

N

A

A

Y

V

C

A

P

P

G

G

I

F

V

I

K

V

T

S

P

D

M

M

M

T

M

D

G

A

I

L

A

S

E

D

Q

E

T

L

A

K

K

-

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L

-

E

E

Q

Q

F

M

S

L

Q

T

N

Q

I

I

T

P

L

L

K

A

R

R

L

F

S

G

E

Q

P

D

E

T

D

D

V

I

A

A

A

N

C

T

V

V

S

A

Y

F

L

L

A

A

G

S

P

D

D

K

S

A

D

K

Y

Y

M

I

T

T

G

G

Q

Q

A

T

L

I

I

H

I

V

D

N

G

G

M

M

active site: G15 (= G17), S141 (= S143), Q151 (≠ L153), Y154 (= Y156), K158 (= K160)
binding magnesium ion: N89 (= N90), K158 (= K160)

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
33% identity, 98% coverage: 5:260/260 of query aligns to 5:252/252 of 1vl8B

query
sites
1vl8B

K

R

Q

G

H

R

V

V

A

A

F

L

V

V

T

T

G
|
G

G

G

G

S

Q
x
R

G
|
G

I
x
L

G

G

K

F

A

G

I

I

C

A

L

Q

R

G

L

L

A

A

Q

E

D

A

G

G

F

C

K

S

I

V

A

V

V

V

A

A

D
x
S

Y
x
R

N

N

F

L

D

E

T

E

A

A

T

S

E

E

V

A

A

A

N

Q

E

K

I

L

N

T

N

E

K

K

E

Y

G

G

-

V

Q

E

A

T

V

M

A

A

I

F

K

R

V
x
C

D
|
D

V
|
V

S

S

D

N

R

Y

E

E

A

E

V

V

F

K

A

K

A

L

V

L

E

E

E

A

A

V

K

K

E

E

K

K

L

F

G

G

G

K

F

L

D

D

V

T

I

V

V

V

N

N

N
x
A

A
|
A

G
|
G

L
x
I

G

N

P

R

Q

R

T

H

P

P

I

A

E

E

I

F

T

P

Y

L

D

D

T

E

Y

F

R

R

R

Q

V

V

F

I

D

E

I
x
V

N

N

V

L

G

F

G

G

T

T

I

Y

W

Y

G

V

M

C

Q

R

A

E

A

A

I

F

K

S

A

L

F

L

K

R

S

E

L

S

G

D

H

N

G

P

G

S

K

I

I

I

I

N

N
x
I

A

G

S
|
S

S

L

Q

T

A

V

G

E

Q

E

V

V

G

T

N

M

P

P

G

N

L
x
I

A

S

V

A

Y
|
Y

G

A

S

A

S

S

K
|
K

F

G

A

G

I

V

R

A

G

S

L

L

T

T

Q

K

T

A

A

L

A

A

K

K

D

E

L

W

A

G

N

R

L

Y

G

G

I

I

T

R

V

V

N

N

A

V

Y

I

C

A

P
|
P

G
|
G

I

W

V
x
Y

K

R

T
|
T

P

K

M
|
M

M
x
T

M

E

G

A

I

V

A

F

E

S

Q

D

T

P

A

E

K

K

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L

L

E

D

Q

Y

F

M

S

L

Q

K

N

R

I

I

T

P

L

L

K

G

R

R

L

T

S

G

E

V

P

P

E

E

D

D

V

L

A

K

A

G

C

V

V

A

S

V

Y

F

L

L

A

A

G

S

P

E

D

E

S

A

D

K

Y

Y

M

V

T

T

G

G

Q

Q

A

I

L

I

I

F

I

V

D

D

G

G

M

W

T

T

F

A

N

N

active site: G17 (= G17), S143 (= S142), I154 (≠ L153), Y157 (= Y156), K161 (= K160)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), R16 (≠ Q16), G17 (= G17), L18 (≠ I18), S37 (≠ D37), R38 (≠ Y38), C63 (≠ V62), D64 (= D63), V65 (= V64), A91 (≠ N90), A92 (= A91), G93 (= G92), I94 (≠ L93), V114 (≠ I113), I141 (≠ N140), S143 (= S142), Y157 (= Y156), K161 (= K160), P187 (= P186), G188 (= G187), Y190 (≠ V189), T192 (= T191), M194 (= M193), T195 (≠ M194)

H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
33% identity, 97% coverage: 8:258/260 of query aligns to 7:242/251 of H9XP47

query
sites
H9XP47

V

V

A

V

F

V

V

I

T

T

G

G

G

A

G

G

Q
x
N

G

G

I
x
M

G

G

K

E

A

A

I

A

C

A

L

R

R

R

L

F

A

S

Q

A

D

E

G

G

F

A

K

I

I

V

A

V

V

L

A

A

D
|
D

Y

W

N

A

F

K

D

E

T

A

A

V

T

D

E

K

V

V

A

A

N

A

E

S

I

L

N

-

K

P

E

K

G

G

Q

R

A

A

V

M

A

A

I

V

K

H

V

I

D
|
D

V
|
V

S

S

D

D

R

H

E

V

A

A

V

V

F

E

A

K

A

M

V

M

E

N

E

E

A

V

K

A

E

E

K

K

L

L

G

G

G

R

F

I

D

D

V

V

I

L

V

L

N

N

N
|
N

A

A

G

G

L

V

G

H

P

V

Q

A

T

G

P

S

I

V

E

L

E

E

I

T

T

S

Y

I

D

D

T

D

Y

W

R

R

V

I

F

A

D

G

I

V

N

D

V

I

G

D

G

G

T

V

I

V

W

F

G

C

M

S

Q

K

A

F

A

A

I

L

K

P

A

H

F

L

K

-

S

-

L

L

G

K

H

T

G

K

G

G

K

C

I

I

I

V

N

N

A

T

S

A

S
|
S

Q
x
V

A
x
S

G

G

Q

L

V

G

G

G

N

D

P

W

G

G

L

A

A

A

V

Y

Y
|
Y

G

C

S

A

K
|
K

F

G

A

A

I

V

R

V

G

N

L

L

T

T

Q

R

T

A

A

M

A

A

K

L

D

D

L

H

A

G

N

G

L

D

G

G

I

V

T

R

V

I

N

N

A

S

Y

V

C

C

P

P

G

S

I

L

V
|
V

K

K

T
|
T

P

N

M

M

M

T

M

N

G

G

I

W

A

P

E

Q

Q

E

T

I

A

-

K

-

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L
x
R

E
x
D

Q
x
K

F

F

S

N

Q

E

N

R

I

I

T

A

L

L

K

G

R

R

L

A

S

A

E

E

P

P

E

E

D

E

V

V

A

A

C

V

V

M

S

A

Y

F

L

L

A

A

G

S

P

D

D

D

S

A

D

S

Y

F

M

I

T

N

G

G

Q

A

A

N

L

I

I

P

I

V

D

D

G

G

M

A

T

T

N15 (≠ Q16) binding NAD(+)
M17 (≠ I18) binding NAD(+)
D36 (= D37) binding NAD(+)
D60 (= D63) binding NAD(+)
V61 (= V64) binding NAD(+)
N87 (= N90) binding NAD(+)
S138 (= S143) binding (R)-acetoin; binding (S)-acetoin
V139 (≠ Q144) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
S140 (≠ A145) binding (R)-acetoin
Y151 (= Y156) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
K155 (= K160) binding NAD(+)
V184 (= V189) binding NAD(+)
T186 (= T191) binding NAD(+)
RDK 197:199 (≠ LEQ 213:215) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.

Sites not aligning to the query:

247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.

6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
33% identity, 97% coverage: 8:258/260 of query aligns to 9:244/252 of 6vspB

query
sites
6vspB

V

V

A

V

F

V

V

I

T

T

G
|
G

G

A

G

G

Q
x
N

G
|
G

I

M

G

G

K

E

A

A

I

A

C

A

L

R

R

R

L

F

A

S

Q

A

D

E

G

G

F

A

K

I

I

V

A

V

V

L

A

A

D
|
D

Y
x
W

N

A

F

K

D

E

T

A

A

V

T

D

E

K

V

V

A

A

N

A

E

S

I

L

N

-

K

P

E

K

G

G

Q

R

A

A

V

M

A

A

I

V

K

H

V
x
I

D
|
D

V
|
V

S

S

D

D

R

H

E

V

A

A

V

V

F

E

A

K

A

M

V

M

E

N

E

E

A

V

K

A

E

E

K

K

L

L

G

G

G

R

F

I

D

D

V

V

I

L

V

L

N

N

A

A

G
|
G

L

V

G

H

P

V

Q

A

T

G

P

S

I

V

E

L

E

E

I

T

T

S

Y

I

D

D

T

D

Y

W

R

R

V

I

F

A

D

G

I

V

N

D

V

I

G

D

G

G

T

V

I

V

W

F

G

C

M

S

Q

K

A

F

A

A

I

L

K

P

A

H

F

L

K

-

S

-

L

L

G

K

H

T

G

K

G

G

K

C

I

I

I

V

N

N

A

T

S

A

S
|
S

Q

V

A

S

G

G

Q

L

V

G

G

G

N

D

P

W

G

G

L

A

A

A

V

Y

Y
|
Y

G

C

S

A

K

K

F

G

A

A

I

V

R

V

G

N

L

L

T

T

Q

R

T

A

A

M

A

A

K

L

D

D

L

H

A

G

N

G

L

D

G

G

I

V

T

R

V

I

N

N

A

S

Y

V

C

C

P

P

G

S

I

L

V

V

K

K

T

T

P

N

M

M

M

T

M

N

G

G

I

W

A

P

E

Q

Q

E

T

I

A

-

K

-

E

-

A

-

G

-

K

-

P

-

F

-

E

-

W

-

G

-

L

R

E

D

Q

K

F

F

S

N

Q

E

N

R

I

I

T

A

L

L

K

G

R

R

L

A

S

A

E

E

P

P

E

E

D

E

V

V

A

A

C

V

V

M

S

A

Y

F

L

L

A

A

G

S

P

D

D

D

S

A

D

S

Y

F

M

I

T

N

G

G

Q

A

A

N

L

I

I

P

I

V

D

D

G

G

M

A