SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_077276850.1 NCBI__GCF_001998885.1:WP_077276850.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26297 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) (see 2 papers)
41% identity, 98% coverage: 5:326/327 of query aligns to 10:331/333 of P26297

query
sites
P26297

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HI 156:157 (≠ RI 151:152) binding NAD(+)
D176 (= D171) binding NAD(+)
H206 (= H201) mutation to Q: Increase of activity.
VP 207:208 (≠ MP 202:203) binding NAD(+)
N213 (= N208) binding NAD(+)
R236 (= R231) mutation to K: Decrease of activity.
D260 (= D255) binding NAD(+); mutation to N: Decrease of activity.
E265 (= E260) mutation to Q: Decrease of activity.
H297 (= H292) mutation to Q: 90% loss of activity.

1j49A Insights into domain closure, substrate specificity and catalysis of d-lactate dehydrogenase from lactobacillus bulgaricus (see paper)
41% identity, 98% coverage: 5:326/327 of query aligns to 10:331/332 of 1j49A

query
sites
1j49A

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active site: S103 (= S97), R236 (= R231), D260 (= D255), E265 (= E260), H297 (= H292)
binding nicotinamide-adenine-dinucleotide: Y102 (= Y96), I107 (= I101), G153 (= G148), G155 (= G150), I157 (= I152), Y175 (= Y170), D176 (= D171), I177 (≠ V172), V207 (≠ M202), P208 (= P203), N213 (= N208), V234 (≠ P229), S235 (≠ A230), R236 (= R231), H297 (= H292), A299 (= A294), F300 (= F295)

2dldA D-lactate dehydrogenase complexed with nadh and oxamate
38% identity, 98% coverage: 5:325/327 of query aligns to 10:330/337 of 2dldA

query
sites
2dldA

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active site: S103 (= S97), R236 (= R231), D260 (= D255), E265 (= E260), H297 (= H292)
binding 1,4-dihydronicotinamide adenine dinucleotide: T154 (= T149), G155 (= G150), H156 (≠ R151), I157 (= I152), D176 (= D171), I177 (≠ V172), V207 (≠ M202), P208 (= P203), N213 (= N208), C234 (≠ P229), S235 (≠ A230), H297 (= H292)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 337

P30901 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
38% identity, 98% coverage: 5:325/327 of query aligns to 10:330/337 of P30901

query
sites
P30901

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S

F

I

N

A

K

E

M

M

K

K

P

D

G

G

A

V

I

V

L

I

L

V

N

N

P

C

A

S

R

R

G

G

A

R

I

L

I

V

D

D

T

T

K

D

D

A

L

V

I

I

A

R

A

G

L

L

D

D

S

S

G

G

Q

K

I

I

S

F

C

G

C

F

A

V

L

M

D
|
D

T

T

Y

Y

E

E

N

D

E

E

M

V

P

G

Y

V

V

F

T

N

K

K

D

D

W

W

S

E

G

G

K

K

T

E

L

F

N

P

D

D

P

K

I

R

L

L

E

A

E

D

L

L

I

I

N

D

R

R

E

P

D

N

V

V

L

L

Y

V

T

T

P

P

H

H

I

T

A

A

F

F

Y

Y

T

T

E

T

T

H

A

A

V

V

E

R

N

N

L

M

V

V

F

V

G

K

G

A

L

F

D

N

A

N

C

N

L

L

S

K

I

L

L

I

T

N

N

G

G

E

T

K

A

P

D

D

T

S

I

P

V

V

D176 (= D171) binding NAD(+)
VP 207:208 (≠ MP 202:203) binding NAD(+)
N213 (= N208) binding NAD(+)
D260 (= D255) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
38% identity, 99% coverage: 3:325/327 of query aligns to 7:328/330 of 1dxyA

query
sites
1dxyA

G

G

A

A

R

R

E

V

D

D

E

E

R

I

R

Q

F

Y

A

F

E

K

K

Q

W

W

A

A

K

K

D

D

H

T

N

G

V

N

T

T

I

L

E

E

V

Y

S

H

T

T

D

E

I

F

L

L

S

D

D

E

D

N

T

T

Y

V

H

E

L

W

L

A

N

K

G

G

Y

F

D

D

G

G

L

I

S

N

L

S

Q

L

Q

Q

T

T

M

T

G

P

I

Y

P

A

A

A

E

G

M

V

Y

F

E

E

K

K

L

M

S

H

Q

A

D

Y

G

G

F

I

R

K

Q

F

I

L

A

T

Q

I

R

R

S

N

A
x
V

G

G

V

T

D

D

M

N

Y

I

D

D

L

M

K

T

E

A

A

M

K

K

K

Q

H

Y

G

G

I

I

S

R

I

L

T

S

N

N

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

N

A

A

A

I

I

A

A

E

E

F

F

T

A

V

L

A

T

S

D

A

T

L

L

N

Y

C

L

L

L

R

R

H

N

M

M

Q

G

A

K

I

V

Q

Q

K

A

R

Q

V

L

K

Q

N

A

H

G

N

D

F

Y

S

E

W

K

D

A

K

G

S

T

I

F

L

I

A

G

R

K

E

E

V

L

R

G

S

Q

L

Q

T

T

I

V

G

G

V

V

M

M

G
|
G

T

T

G
|
G

R
x
H

I
|
I

G

G

Q

Q

I

V

T

A

A

I

Q

K

L

L

F

F

A

K

A

G

F

F

G

G

A

A

K

K

V

V

I

I

G

A

Y
|
Y

D
|
D

V
x
P

Y

Y

Q

P

N

M

P

K

N

G

A

D

E

H

N

P

Y

D

L

F

T

D

Y

Y

I

V

D

-

S

S

F

L

D

E

E

D

F

L

L

F

A

K

Q

Q

S

S

D

D

L

V

L

I

T

D

I

L

H
|
H

M
x
V

P
|
P

L

G

T

I

D

E

D

Q

N
|
N

Y

T

H

H

Q

I

F

I

N

N

T

E

E

A

T

A

F

F

N

N

K

L

M

M

K

K

P

P

G

G

A

A

I

I

L

V

L

I

N

N

P
x
T

A
|
A

R
|
R

G

P

A

N

I

L

I

I

D

D

T

T

K

Q

D

A

L

M

I

L

A

S

A

N

L

L

D

K

S

S

G

G

Q

K

I

L

S

A

C

G

C

V

A

G

L

I

D
|
D

T

T

Y

Y

E

E

N

Y

E
|
E

M

T

P

E

Y

D

V

L

T

L

K

N

D

L

W

A

S

K

G

H

K

G

T

S

L

F

N

K

D

D

P

P

I

L

L

W

E

D

E

E

L

L

I

L

N

G

R

M

E

P

D

N

V

V

L

V

Y

L

T

S

P

P

H
|
H

I

I

A

A

F
x
Y

Y

Y

T

T

E

E

T

T

A

A

V

V

E

H

N

N

L

M

V

V

F

Y

G

F

G

S

L

L

D

Q

A

H

C

L

L

V

S

D

I

F

L

L

T

T

N

K

G

G

T

E

A

T

D

S

T

T

I

E

V

V

active site: S101 (= S97), R234 (= R231), D258 (= D255), E263 (= E260), H295 (= H292)
binding 2-oxo-4-methylpentanoic acid: V77 (≠ A73), Y100 (= Y96), Y298 (≠ F295)
binding nicotinamide-adenine-dinucleotide: Y100 (= Y96), G152 (= G148), G154 (= G150), H155 (≠ R151), I156 (= I152), Y174 (= Y170), D175 (= D171), P176 (≠ V172), H204 (= H201), V205 (≠ M202), P206 (= P203), N211 (= N208), T232 (≠ P229), A233 (= A230), R234 (= R231), H295 (= H292), Y298 (≠ F295)

P17584 D-2-hydroxyacid dehydrogenase (NAD+); D-2-hydroxyisocaproate dehydrogenase; D-HicDH; EC 1.1.1.345 from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
38% identity, 99% coverage: 3:325/327 of query aligns to 7:328/333 of P17584

query
sites
P17584

G

G

A

A

R

R

E

V

D

D

E

E

R

I

R

Q

F

Y

A

F

E

K

K

Q

W

W

A

A

K

K

D

D

H

T

N

G

V

N

T

T

I

L

E

E

V

Y

S

H

T

T

D

E

I

F

L

L

S

D

D

E

D

N

T

T

Y

V

H

E

L

W

L

A

N

K

G

G

Y

F

D

D

G

G

L

I

S

N

L

S

Q

L

Q

Q

T

T

M

T

G

P

I

Y

P

A

A

A

E

G

M

V

Y

F

E

E

K

K

L

M

S

H

Q

A

D

Y

G

G

F

I

R

K

Q

F

I

L

A

T

Q

I

R

R

S

N

A

V

G

G

V

T

D

D

M

N

Y

I

D

D

L

M

K

T

E

A

A

M

K

K

K

Q

H

Y

G

G

I

I

S

R

I

L

T

S

N

N

V

V

P

P

A

A

Y
|
Y

S

S

P

P

N

A

A

A

I

I

A

A

E

E

F

F

T

A

V

L

A

T

S

D

A

T

L

L

N

Y

C

L

L

L

R

R

H

N

M

M

Q

G

A

K

I

V

Q

Q

K

A

R

Q

V

L

K

Q

N

A

H

G

N

D

F

Y

S

E

W

K

D

A

K

G

S

T

I

F

L

I

A

G

R

K

E

E

V

L

R

G

S

Q

L

Q

T

T

I

V

G

G

V

V

M

M

G

G

T

T

G

G

R
x
H

I
|
I

G

G

Q

Q

I

V

T

A

A

I

Q

K

L

L

F

F

A

K

A

G

F

F

G

G

A

A

K

K

V

V

I

I

G

A

Y

Y

D
|
D

V

P

Y

Y

Q

P

N

M

P

K

N

G

A

D

E

H

N

P

Y

D

L

F

T

D

Y

Y

I

V

D

-

S

S

F

L

D

E

E

D

F

L

L

F

A

K

Q

Q

S

S

D

D

L

V

L

I

T

D

I

L

H

H

M
x
V

P

P

L

G

T

I

D

E

D

Q

N
|
N

Y

T

H

H

Q

I

F

I

N

N

T

E

E

A

T

A

F

F

N

N

K

L

M

M

K

K

P

P

G

G

A

A

I

I

L

V

L

I

N

N

P
x
T

A

A

R
|
R

G

P

A

N

I

L

I

I

D

D

T

T

K

Q

D

A

L

M

I

L

A

S

A

N

L

L

D

K

S

S

G

G

Q

K

I

L

S

A

C

G

C

V

A

G

L

I

D
|
D

T

T

Y

Y

E

E

N

Y

E

E

M

T

P

E

Y

D

V

L

T

L

K

N

D

L

W

A

S

K

G

H

K

G

T

S

L

F

N

K

D

D

P

P

I

L

L

W

E

D

E

E

L

L

I

L

N

G

R

M

E

P

D

N

V

V

L

V

Y

L

T

S

P

P

H
|
H

I

I

A

A

F

Y

Y

Y

T

T

E

E

T

T

A

A

V

V

E

H

N

N

L

M

V

V

F

Y

G

F

G

S

L

L

D

Q

A

H

C

L

L

V

S

D

I

F

L

L

T

T

N

K

G

G

T

E

A

T

D

S

T

T

I

E

V

V

Y100 (= Y96) binding 4-methyl-2-oxopentanoate
H155 (≠ R151) binding NAD(+)
I156 (= I152) binding NAD(+)
D175 (= D171) binding NAD(+)
V205 (≠ M202) binding NAD(+)
N211 (= N208) binding NAD(+)
T232 (≠ P229) binding NAD(+)
R234 (= R231) binding NAD(+)
D258 (= D255) binding NAD(+)
H295 (= H292) binding 4-methyl-2-oxopentanoate

4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
39% identity, 100% coverage: 1:326/327 of query aligns to 5:329/332 of 4xkjA

query
sites
4xkjA

M

M

M

F

G

S

A

V

R

R

E

D

D

D

E

E

R

E

R

A

F

A

A

I

E

R

K

E

W

W

A

E

K

K

D

K

H

T

N

G

V

V

T

Q

I

V

E

D

V

I

S

N

T

R

D

L

I

E

L

L

S

D

D

A

D

E

T

T

Y

A

H

Q

L

L

L

T

N

K

G

G

Y

Y

D

D

G

G

L

I

S

V

L

I

Q

Q

T

R

M

S

G

H

I

I

-

S

-

N

P

P

A

A

E

-

M

V

Y

Y

E

E

K

T

L

L

S

Q

Q

K

D

N

G

G

F

L

R

R

Q

Q

I

L

A

T

Q

S

R

R

S

T

A

A

G

G

V

Y

D

D

M

M

Y

I

D

D

L

L

K

E

E

Q

A

A

K

S

K

E

H

R

G

G

I

L

S

V

I

V

T

T

N

N

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

N

N

A

S

I
x
V

A

A

E

E

F

L

T

A

V

L

A

T

S

Q

A

T

L

M

N

R

C

L

L

I

R

R

H

N

M

L

Q

P

A

L

I

F

Q

D

K

A

R

R

V

G

K

A

N

E

H

Q

N

D

F

F

S

R

W

W

D

-

K

A

S

G

I

L

L

M

A

A

R

R

E

E

V

I

R

R

S

S

L

L

T

T

I

V

G

G

V

I

M

I

G
|
G

T

A

G
|
G

R
|
R

I
|
I

G

G

Q

G

I

T

T

V

A

A

Q

R

L

L

F

F

A

K

A

A

F

L

G

G

A

A

K

T

V

V

I

I

G

A

Y

N

D
|
D

V
x
I

Y

V

Q

E

N
x
R

P

V

N

E

A

L

E

K

N

D

Y

I

L

V

T

T

Y

Y

I

V

D

-

S

S

F

K

D

E

E

E

F

L

L

L

A

Q

Q

A

S

A

D

D

L

V

T

T

I

L

H
|
H

M
x
V

P
|
P

L

L

T

M

D

D

D

S

N
x
T

Y

T

H

Q

Q

L

F

I

N

D

T

A

E

D

T

A

F

L

N

A

K

L

M

M

K

K

P

N

G

D

A

A

I

V

L

L

L

I

N

N

P
x
A

A

S

R
|
R

G

G

A

P

I

V

D

D

T

T

K

D

D

A

L

L

I

I

A

A

L

L

D

Q

S

N

G

K

Q

Q

I

I

S

A

C

G

C

A

A

A

L

L

D
|
D

T

T

Y

L

E

N

N

G

E
|
E

M

E

P

H

Y

F

V

F

T

N

K

Q

D

D

W

L

S

C

G

G

K

K

T

E

L

L

N

P

D

S

P

E

I

Q

L

L

E

K

E

V

L

L

I

R

N

T

R

L

E

P

D

N

V

V

L

L

Y

I

T

T

P

P

H
|
H

I

I

A
x
G

F
|
F

Y

Y

T

T

E

N

T

K

A

A

V

V

E

Q

N

N

L

M

V

V

F

E

G

I

G

S

L

L

D

N

A

D

C

V

L

L

S

A

I

I

L

L

T

K

N

T

G

G

T

T

A

S

D

E

T

H

I

Q

V

L

N

N

active site: S102 (= S97), R234 (= R231), D258 (= D255), E263 (= E260), H295 (= H292)
binding nicotinamide-adenine-dinucleotide: Y101 (= Y96), V106 (≠ I101), G152 (= G148), G154 (= G150), R155 (= R151), I156 (= I152), D175 (= D171), I176 (≠ V172), R179 (≠ N175), H204 (= H201), V205 (≠ M202), P206 (= P203), T211 (≠ N208), A232 (≠ P229), R234 (= R231), H295 (= H292), G297 (≠ A294), F298 (= F295)

2yq5C Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from lactobacillus delbrueckii ssp. Bulgaricus: NAD complexed form (see paper)
37% identity, 98% coverage: 8:326/327 of query aligns to 12:331/331 of 2yq5C

query
sites
2yq5C

E

E

R

V

R

P

F

Y

A

I

E

E

K

D

W

W

A

A

K

K

D

K

H

N

N

D

V

V

T

E

I

I

E

K

V

T

S

T

T

D

D

Q

I

A

L

L

S

T

D

S

D

A

T

T

Y

V

H

D

L

L

L

A

N

E

G

G

Y

C

D

S

G

S

L

V

S

S

L

L

Q

K

Q

P

T

L

M

G

G

P

I

V

P

D

A

E

E

E

M

V

-

V

Y

Y

E

Q

K

K

L

L

S

S

Q

E

D

Y

G

G

F

V

R

K

Q

C

I

I

A

G

Q

L

R

R

S

I

A

V

G

G

V

F

D

N

M

T

Y

I

D

N

L

F

K

D

E

W

A

T

K

K

H

Y

G

N

I

L

S

L

I

V

T

T

N

N

V

V

P

P

A

V

Y
|
Y

S
|
S

P

P

N

R

A

A

I
|
I

A

A

E

E

F

M

T

T

V

V

A

T

S

Q

A

A

L

M

N

Y

C

L

L

L

R

R

H

K

M

I

Q

G

A

E

I

F

Q

R

K

Y

R

R

V

M

-

D

K

H

N

D

H

H

N

D

F

F

S

T

W

W

D

P

K

S

S

N

I

L

L

I

A

S

R

N

E

E

V

I

R

Y

S

N

L

L

T

T

I

V

G

G

V

L

M

I

G

G

T
x
V

G
|
G

R
x
H

I
|
I

G

G

Q

S

I

A

T

V

A

A

Q

E

L

I

F

F

A

S

A

A

F

M

G

G

A

A

K

K

V

V

I

I

G

A

Y
|
Y

D
|
D

V
|
V

Y

A

Q

Y

N

N

P

P

N

E

A

F

E

E

N

P

Y

F

L

L

T

T

Y

Y

I

T

D

D

S

-

F

F

D

D

E

T

F

V

L

L

A

K

Q

E

S

A

D

D

L

I

L

V

T

S

I

L

H
|
H

M
x
T

P
|
P

L

L

T

F

D

P

D

S

N

T

Y

E

H

N

Q

M

F

I

N

G

T

E

E

K

T

Q

F

L

N

K

K

E

M

M

K

K

P

K

G

S

A

A

I

Y

L

L

L

I

N

N

P

C

A
|
A

R
|
R

G

G

A

E

I

L

I

V

D

D

T

T

K

G

D

A

L

L

I

I

A

K

A

A

L

L

D

Q

S

D

G

G

Q

E

I

I

S

A

C

G

C

A

A

G

L

L

D
|
D

T

T

Y

L

E

A

N

G

E
|
E

M

S

P

S

Y

Y

V

-

T

-

K

-

D

-

W

F

S

G

G

H

K

T

T

G

L

L

N

T

D

D

P

S

I

E

L

I

E

P

E

E

-

D

-

Y

-

K

-

T

L

L

I

A

N

K

R

M

E

P

D

N

V

V

L

V

Y

I

T

T

P

P

H
|
H

I

S

A

A

F
|
F

Y

Y

T

T

E

E

T

T

A

S

V

I

E

R

N

N

L

M

V

V

F

Q

G

I

G

C

L

L

D

T

A

D

C

Q

L

L

S

T

I

I

L

A

T

K

N

G

G

G

T

R

A

P

D

R

T

S

I

I

V

V

N

N

active site: S102 (= S97), R236 (= R231), D260 (= D255), E265 (= E260), H297 (= H292)
binding nicotinamide-adenine-dinucleotide: Y101 (= Y96), I106 (= I101), V155 (≠ T149), G156 (= G150), H157 (≠ R151), I158 (= I152), Y176 (= Y170), D177 (= D171), V178 (= V172), H206 (= H201), T207 (≠ M202), P208 (= P203), A235 (= A230), R236 (= R231), H297 (= H292), F300 (= F295)

3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
39% identity, 79% coverage: 46:304/327 of query aligns to 40:306/334 of 3kb6B

query
sites
3kb6B

L

L

Q

K

T

A

M

E

G

L

I

I

P

S

A

V

E
x
F

M

V

Y

Y

E

D

K

K

L

L

S

T

Q

E

D

E

-

L

-

S

-

K

-

M

-

P

G

R

F

L

R

K

Q

L

I

I

A

H

Q

T

R

R

S
|
S

A
x
V

G
|
G

V

F

D

D

M

H

Y

I

D

D

L

L

K

D

E

Y

A

C

K

K

H

K

G

G

I

I

S

L

I

V

T

T

N

H

V

I

P

P

A

A

Y
|
Y

S
|
S

P

P

N

E

A

S

I
x
V

A

A

E

E

F

H

T

T

V

F

A

A

S

M

A

I

L

L

N

T

C

L

L

V

R

K

H

R

M

L

Q

K

A

R

I

I

Q

E

K

D

R

R

V

V

K

K

N

K

H

L

N

N

F

F

S

S

W

Q

D

D

K

S

S

E

I

I

L

L

A

A

R

R

E

E

V

L

R

N

S

R

L

L

T

T

I

L

G

G

V

V

M

I

G

G

T

T

G
|
G

R
|
R

I
|
I

G

G

Q

S

I

R

T

V

A

A

Q

M

L

Y

F

G

A

L

A

A

F

F

G

G

A

M

K

K

V

V

I

L

G

C

Y

Y

D
|
D

V
|
V

Y

V

Q

K

N

R

P

E

N

D

A

L

E

K

N

E

Y

K

L

G

T

C

Y

V

I

Y

D

T

S

S

F

L

D

D

E

E

F

L

L

L

A

K

Q

E

S

S

D

D

L

V

L

I

T

S

I

L

H

H

M

V

P
|
P

L

Y

T

T

D

K

D

E

N

T

Y

H

H

H

Q

M

F

I

N

N

T

E

E

E

T

R

F

I

N

S

K

L

M

M

K

K

P

D

G

G

A

V

I

Y

L

L

L

I

N

N

P
x
T

A
|
A

R
|
R

G

G

A

K

I

V

D

D

T

T

K

D

D

A

L

L

I

Y

A

R

A

A

L

Y

D

Q

S

R

G

G

Q

K

I

F

S

S

C

G

C

L

A

G

L

L

D
|
D

T

V

Y

F

E

E

N

D

E
|
E

M

E

P

I

Y

L

V

I

T

L

K

K

D

K

W

Y

S

T

G

E

K

G

T

K

L

A

N

T

D

D

P

K

I

N

L

L

E

K

-

I

-

L

E

E

L

L

I

A

N

C

R

K

E

D

D

N

V

V

L

I

Y

I

T

T

P

P

H
|
H

I

I

A
|
A

F
x
Y

Y

Y

T

T

E

D

T

K

A

S

V

L

E

E

N

R

L

I

active site: S97 (= S97), R231 (= R231), D255 (= D255), E260 (= E260), H294 (= H292)
binding lactic acid: F49 (≠ E55), S72 (= S72), V73 (≠ A73), G74 (= G74), Y96 (= Y96), R231 (= R231), H294 (= H292)
binding nicotinamide-adenine-dinucleotide: V73 (≠ A73), Y96 (= Y96), V101 (≠ I101), G150 (= G150), R151 (= R151), I152 (= I152), D171 (= D171), V172 (= V172), P203 (= P203), T229 (≠ P229), A230 (= A230), R231 (= R231), H294 (= H292), A296 (= A294), Y297 (≠ F295)

4prlA Crystal structure of d-lactate dehydrogenase with NAD+ from lactobacillus jensenii (see paper)
34% identity, 96% coverage: 6:319/327 of query aligns to 10:321/330 of 4prlA

query
sites
4prlA

E

E

D

S

E

E

R

R

R

K

F

L

A

V

E

A

K

A

W

T

A

E

K

K

D

K

H

F

N

G

V

C

T

E

I

L

E

K

V

L

S

I

T

D

D

E

I

S

L

L

S

S

D

A

D

E

T

N

Y

V

H

D

L

Q

L

V

N

A

G

D

Y

C

D

D

G

G

L

V

S

L

L

L

Q

K

Q

P

T

L

M

G

G

N

I

L

P

D

A

D

E

E

M

I

-

V

Y

Y

E

K

K

K

L

L

S

A

Q

D

D

Y

G

G

F

I

R

K

Q

S

I

I

A

G

Q

L

R

R

S

I

A

V

G

G

V

T

D

N

M

T

Y

I

D

D

L

F

K

D

E

L

A

A

K

K

H

Y

G

H

I

L

S

T

I

V

T

T

N

N

V

V

P

P

A

V

Y
|
Y

S

S

P

P

N

R

A

A

I
|
I

A

A

E

E

F

M

T

A

V

V

A

T

S

Q

A

A

L

M

N

Y

C

L

L

N

R

R

H

K

M

I

Q

G

A

E

I

F

Q

K

K

A

R

N

V

M

K

D

N

K

H

G

N

D

F

F

S

T

W

N

D

P

K

D

S

S

I

L

L

I

A

S

R

N

E

E

V

I

R

Y

S

N

L

K

T

T

I

I

G

G

V

L

M

I

G

G

T
x
V

G
|
G

R
x
H

I
|
I

G

G

Q

S

I

A

T

V

A

A

Q

Q

L

I

F

F

A

S

A

A

F

M

G

G

A

A

K

K

V

V

I

L

G

A

Y
|
Y

D
|
D

V

V

Y

I

Q

Y

N

N

P

P

N

E

A

V

E

E

N

P

Y

Y

L

L

T

T

Y

Y

I

A

D

D

S

-

F

F

D

D

E

T

F

V

L

L

A

K

Q

E

S

A

D

D

L

I

T

S

I

L

H
|
H

M
x
T

P
|
P

L

L

T

L

D

K

D

S

N

T

Y

E

H

N

Q

M

F

I

N

G

T

K

E

K

T

Q

F

F

N

A

K

E

M

M

K

K

P

N

G

D

A

A

I

I

L

L

L

I

N

N

P
x
A

A
|
A

R

R

G

G

A

E

I

L

I

V

D

D

T

T

K

A

D

A

L

L

I

I

A

E

A

A

L

L

D

E

S

K

G

H

Q

E

I

I

S

A

C

A

A

G

L

L

D
|
D

T

T

Y

L

E

A

N

H

E

E

M

S

P

S

Y

Y

V

F

T

F

K

K

D

K

W

V

S

D

G

D

K

A

T

Q

L

I

N

P

D

A

P

D

I

-

L

Y

E

K

L

L

I

A

N

A

R

M

E

P

D

N

V

V

L

I

Y

V

T

T

P

P

H
|
H

I

S

A
|
A

F

Y

Y

F

T

T

E

K

T

T

A

S

V

V

E

R

N

N

L

M

V

I

F

E

G

I

G

S

L

L

D

R

A

D

C

T

L

I

S

A

I

-

L

L

T

A

N

N

G

G

binding nicotinamide-adenine-dinucleotide: Y101 (= Y96), I106 (= I101), V154 (≠ T149), G155 (= G150), H156 (≠ R151), I157 (= I152), Y175 (= Y170), D176 (= D171), H205 (= H201), T206 (≠ M202), P207 (= P203), A233 (≠ P229), A234 (= A230), D259 (= D255), H295 (= H292), A297 (= A294)

4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
38% identity, 84% coverage: 30:304/327 of query aligns to 44:323/346 of 4zgsA

query
sites
4zgsA

L

L

S

D

D

K

D

D

T

T

Y

A

H

Q

L

L

L

A

N

R

G

G

Y

Y

D

D

G

V

L

A

S

V

L

L

Q

F

Q

V

T

N

M

D

G

R

I

A

P

D

A

A

E

S

M

V

Y

I

E

K

K

E

L

L

S

A

Q

K

D

A

G

G

F

V

R

K

Q

L

I

I

A

A

Q

L

R

R

S

C

A

A

G

G

V

F

D

D

M

R

Y

V

D

D

L

L

K

H

E

A

A

C

K

A

K

E

H

H

G

G

I

V

S

R

I

V

T

V

N

R

V

V

P

P

A

T

Y
|
Y

S
|
S

P

P

N

E

A

S

I

V

A

A

E

E

F

H

T

A

V

V

A

A

S

L

A

I

L

F

N

A

C

L

L

N

R

R

H

H

M

L

Q

T

A

D

I

A

Q

Y

K

I

R

R

V

V

K

R

N

M

H

G

N

N

F

Y

S

S

W

L

D

S

K

-

S

G

I

L

L

V

A

G

R

V

E

E

V

M

R

R

S

H

L

K

T

V

I

V

G

G

V

V

M

V

G

G

T

T

G
|
G

R
x
A

I
|
I

G

G

Q

Q

I

Q

T

A

A

A

Q

R

L

I

F

L

A

K

A

G

F

I

G

G

A

C

K

K

V

V

I

F

G

A

Y

Y

D
|
D

V

I

Y

K

Q

P

N

N

P

P

N

A

A

V

E

E

N

A

Y

M

L

G

T

I

Y

P

I

Y

D

V

S

S

F

L

D

D

E

E

F

L

L

L

A

A

Q

M

S

S

D

D

L

I

L

V

T

T

I

L

H

H

M
x
C

P
|
P

L

L

T
x
L

D

P

D
x
S

N
x
T

Y

R

H

Q

Q

L

F

I

N

N

T

K

E

E

T

S

F

I

N

Q

K

K

M

M

K

K

P

K

G

G

A

V

I

M

L

L

L

I

N

N

P

V

A
x
S

R
|
R

G

G

A

G

I

L

I

I

D

D

T

S

K

A

D

A

L

L

I

F

A

D

A

A

L

L

D

E

S

S

G

G

Q

Q

I

I

S

G

C

A

C

L

A

G

L

L

D
|
D

T

V

Y

Y

E

E

N

N

E
|
E

-

G

-

L

-

F

M

V

P

D

Y

H

V

T

T

K

K

F

D

D

W

P

S

S

G

V

K

R

T

M

L

Q

N

K

-

W

D

D

P

R

I

Q

L

F

E

R

E

T

L

L

I

L

N

S

R

Y

E

P

D

Q

V

V

L

L

Y

V

T

T

P

P

H
|
H

I

T

A

A

F
|
F

Y

L

T

T

E

E

T

E

A

A

V

L

E

N

N

N

L

I

active site: S111 (= S97), R244 (= R231), D268 (= D255), E273 (= E260), H311 (= H292)
binding nicotinamide-adenine-dinucleotide: Y110 (= Y96), G163 (= G150), A164 (≠ R151), I165 (= I152), D184 (= D171), C215 (≠ M202), P216 (= P203), L218 (≠ T205), S220 (≠ D207), T221 (≠ N208), S243 (≠ A230), H311 (= H292), F314 (= F295)

5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
34% identity, 89% coverage: 19:310/327 of query aligns to 30:320/336 of 5z20F

query
sites
5z20F

H

H

N

G

V

F

T

E

I

L

E

H

V

F

S

Q

T

Q

D

A

I

H

L

L

S

Q

D

A

D

D

T

T

Y

A

H

V

L

L

L

A

N

Q

G

G

Y

F

D

E

G

V

L

V

S

C

L

A

Q

F

Q

V

T

N

M

D

G

D

I

L

P

S

A

R

E

P

M

V

Y

L

E

E

K

R

L

L

S

A

Q

A

D

G

G

G

F

T

R

R

Q

L

I

V

A

A

Q

L

R

R

S

S

A

A

G

G

V

Y

D

N

M

H

Y

V

D

D

L

L

K

A

E

A

A

A

K

E

K

A

H

L

G

G

I

L

S

P

I

V

T

V

N

H

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

N

H

A

A

I

V

A

A

E

E

F

H

T

A

V

V

A

G

S

L

A

I

L

L

N

T

C

L

L

N

R

R

H

R

M

L

Q

H

A

R

I

A

Q

Y

K

N

R

R

V

T

K

R

N

E

H

G

N

D

F

F

S

S

W

L

D

-

K

H

S

G

I

L

L

T

A

G

R

F

E

D

V

L

R

H

S

G

L

K

T

R

I

V

G

G

V

V

M

I

G

G

T

T

G
|
G

R
x
Q

I
|
I

G

G

Q

E

I

T

T

F

A

A

Q

R

L

I

F

M

A

A

A

G

F

F

G

G

A

C

K

E

V

L

I

L

G

A

Y
|
Y

D
|
D

V
x
P

Y

Y

Q

P

N

N

P

P

N

R

A

I

E

Q

N

A

Y

L

L

G

T

G

Y

R

I

Y

D

L

S

A

F

L

D

D

E

A

F

L

L

L

A

A

Q

E

S

S

D

D

L

I

L

V

T

S

I

L

H

H

M
x
C

P
|
P

L

L

T

T

D

A

D

D

N
x
T

Y

R

H

H

Q

L

F

I

N

D

T

A

E

Q

T

R

F

L

N

A

K

T

M

M

K

K

P

P

G

G

A

A

I

M

L

L

L

I

N

N

P
x
T

A
x
G

R
|
R

G

G

A

A

I

L

I

V

D

N

T

A

K

A

D

A

L

L

I

I

A

E

A

A

L

L

D

K

S

S

G

G

Q

Q

I

L

S

G

C

Y

C

L

A

G

L

L

D
|
D

T

V

Y

Y

E

E

N

E

E
|
E

M

A

P

D

Y

I

V

F

T

F

K

E

D

D

W

R

S

S

G

D

K

Q

T

P

L

L

N

Q

D

D

P

D

I

V

L

L

E

A

E

R

L

L

I

L

N

S

R

F

E

P

D

N

V

V

L

V

Y

V

T

T

P

A

H
|
H

I

Q

A
|
A

F

F

Y

L

T

T

E

R

T

E

A

A

V

L

E

A

N

A

L

I

V

A

F

D

G

T

L

L

D

D

active site: S108 (= S97), R241 (= R231), D265 (= D255), E270 (= E260), H302 (= H292)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y96), G160 (= G150), Q161 (≠ R151), I162 (= I152), Y180 (= Y170), D181 (= D171), P182 (≠ V172), C212 (≠ M202), P213 (= P203), T218 (≠ N208), T239 (≠ P229), G240 (≠ A230), R241 (= R231), H302 (= H292), A304 (= A294)

5z21B The ternary structure of d-lactate dehydrogenase from fusobacterium nucleatum with nadh and oxamate (see paper)
34% identity, 91% coverage: 5:301/327 of query aligns to 10:305/331 of 5z21B

query
sites
5z21B

R

K

E

D

D

Y

E

D

R

K

R

E

F

F

A

F

E

K

K

K

W

Y

A

G

K

A

D

D

H

Y

N

N

V

F

T

E

I

M

E

T

V

F

S

L

T

K

D

V

I

R

L

L

S

T

D

E

T

T

Y

A

H

N

L

L

L

T

N

K

G

G

Y

Y

D

D

G

V

L

V

S

C

L

G

Q

F

Q

A

T

N

M

D

G

N

I

I

P

N

A

K

E

E

M

T

Y

I

E

D

K

I

L

M

S

A

Q

E

D

N

G

G

F

I

R

K

Q

L

I

L

A

A

Q

M

R

R

S

C

A

A

G

G

V

F

D

N

M

N

Y

V

D

S

L

L

K

K

E

D

A

V

K

N

K

E

H

R

G

-

I

F

S

K

I

V

T

V

N

R

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

N

H

A

A

I
|
I

A

A

E

E

F

Y

T

T

V

V

A

G

S

L

A

I

L

L

N

A

C

V

L

N

R

R

H

K

M

I

Q

N

A

K

I

A

Q

Y

K

V

R

R

V

T

K

R

N

E

H

G

N

N

F

F

S

S

W

I

D

N

K

-

S

G

I

L

L

M

A

G

R

I

E

D

V

L

R

Y

S

E

L

K

T

T

I

A

G

G

V

I

M

I

G

G

T

T

G
|
G

R
x
K

I
|
I

G

G

Q

Q

I

I

T

L

A

I

Q

K

L

I

F

L

A

R

A

G

F

F

G

D

A

M

K

K

V

V

I

I

G

A

Y

Y

D
|
D

V
x
L

Y

F

Q

P

N

N

P

Q

N

K

A

V

E

A

N

D

Y

E

L

L

T

G

Y

F

-

E

I

Y

D

V

S

S

F

L

D

D

E

E

F

L

L

Y

A

A

Q

N

S

S

D

D

L

I

T

S

I

L

H

N

M

C

P
|
P

L

L

T

T

D

K

D

D

N
x
T

Y

K

H

Y

Q

M

F

I

N

N

T

R

E

R

T

S

F

M

N

L

K

K

M

M

K

K

P

D

G

G

A

V

I

I

L

L

L

V

N

N

P
x
T

A
x
G

R
|
R

G

G

A

M

I

L

I

I

D

D

T

S

K

A

D

D

L

L

I

V

A

E

A

A

L

L

D

K

S

D

G

K

Q

K

I

I

S

G

C

A

C

V

A

A

L

L

D
|
D

T

V

Y

Y

E

E

N

E

E
|
E

M

E

P

N

Y

Y

V

F

T

F

K

E

D

D

W

K

S

S

G

T

K

Q

T

V

L

I

N

E

D

D

P

D

I

I

L

L

E

G

E

R

L

L

I

L

N

S

R

F

E

Y

D

N

V

V

L

L

Y

I

T

T

P

S

H
|
H

I

Q

A

A

F
x
Y

Y

F

T

T

E

K

T

E

A

A

V

V

active site: S101 (= S97), R235 (= R231), D259 (= D255), E264 (= E260), H296 (= H292)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y96), I105 (= I101), G153 (= G150), K154 (≠ R151), I155 (= I152), D174 (= D171), L175 (≠ V172), P207 (= P203), T212 (≠ N208), T233 (≠ P229), G234 (≠ A230), R235 (= R231), H296 (= H292), Y299 (≠ F295)

8grvA Dictyostelium discoideum lactate dehydrogenase (dicldha)with NAD
35% identity, 76% coverage: 52:301/327 of query aligns to 57:307/336 of 8grvA

query
sites
8grvA

I

L

P

S

A

K

E

E

M

V

Y

I

E

E

K

T

L

L

S

H

Q

S

D

N

G

G

F

T

R

K

Q

V

I

I

A

L

Q

M

R

R

S

C

A

A

G

G

V

F

D

N

M

K

Y

V

D

D

L

L

K

D

E

T

A

A

K

N

K

K

H

L

G

G

I

I

S

P

I

V

T

L

N

R

V

V

P

P

A

A

Y

Y

S

S

P

P

N

N

A

A

I
x
V

A

S

E

E

F

Y

T

A

V

L

A

S

S

L

A

I

L

M

N

A

C

L

L

N

R

R

H

K

M

T

Q

H

A

K

I

A

Q

H

K

D

R

R

V

V

K

R

N

D

H

A

N

N

F

F

S

E

W

I

D

N

K

-

S

G

I

M

L

E

A

G

R

F

E

N

V

M

R

V

S

S

L

K

T

V

I

Y

G

G

V

I

M

V

G

G

T

T

G
|
G

R
x
N

I
|
I

G

G

-

E

Q

Q

I

L

T

C

A

R

Q

V

L

L

F

K

A

L

A

G

F

F

G

G

A

A

K

K

V

V

I

I

G

A

Y

Y

D
|
D

V
x
I

Y
x
I

Q

E

N

N

P

K

N

A

A

V

E

T

N

D

Y

I

-

G

L

I

T

E

Y

Y

I

V

D

K

S

T

F

L

D

D

E

E

F

I

L

W

A

K

Q

Q

S

C

D

D

L

V

L

I

T

S

I

L

H

H

M
x
T

P
|
P

L

L

T

N

D

S

D

Q

N
x
T

Y

K

H

Y

Q

M

F

V

N

N

T

S

E

E

T

S

F

I

N

E

K

K

M

M

K

R

P

D

G

G

A

V

I

M

L

I

N

N

P
x
V

A

S

R

R

G

G

A

A

I

L

I

V

D

N

T

A

K

S

D

D

L

A

I

I

A

V

A

G

L

L

D

K

S

S

G

G

Q

K

I

I

S

S

C

S

C

L

A

G

L

M

D

D

T

V

Y

Y

E

E

N

N

E

E

M

T

P

D

Y

Y

V

F

T

Y

K

Q

D

D

W

H

S

N

G

G

K

S

T

I

L

I

N

K

D

D

P

D

I

N

L

L

E

S

E

L

L

L

I

I

N

S

R

Y

E

P

D

N

V

V

L

M

Y

I

T

T

P

S

H
|
H

I

Q

A
|
A

F
x
W

Y

Y

T

T

E

K

T

E

A

A

V

I

binding nicotinamide-adenine-dinucleotide: V106 (≠ I101), G154 (= G150), N155 (≠ R151), I156 (= I152), D176 (= D171), I177 (≠ V172), I178 (≠ Y173), T208 (≠ M202), P209 (= P203), T214 (≠ N208), V235 (≠ P229), H298 (= H292), A300 (= A294), W301 (≠ F295)

4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
30% identity, 92% coverage: 5:304/327 of query aligns to 9:315/330 of 4cukA

query
sites
4cukA

R

K

E

Q

D

Y

E

D

R

K

F

Y

A

L

E

Q

K

Q

W

V

A

N

K

E

D

A

H

F

N

G

V

F

T

E

I

L

E

E

V

F

S

F

T

D

D

F

I

L

L

L

S

T

D

E

D

K

T

T

Y

A

H

K

L

T

L

A

N

N

G

G

Y

C

D

E

G

A

L

V

S

C

L

I

Q

F

Q

V

T

N

M

D

G

D

I

G

P

S

A

R

E

P

M

V

Y

L

E

E

K

E

L

L

S

K

Q

K

D

H

G

G

F

V

R

K

Q

Y

I

I

A

A

Q

L

R

R

S

C

A

A

G

G

V

F

D

N

M

N

Y

V

D

D

L

L

K

D

E

A

A

A

K

K

K

E

H

L

G

G

I

L

S

Q

I

V

T

V

N

R

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

N

E

A

A

I

V

A

A

E

E

F

H

T

A

V

I

A

G

S

M

A

M

L

M

N

T

C

L

L

N

R

R

H

R

M

I

Q

H

A

R

I

A

Q

Y

K

Q

R

R

V

T

K

R

N

D

H

A

N

N

F

F

S

S

W

L

D

E

K

-

S

G

I

L

L

T

A

G

R

F

E

T

V

M

R

H

S

G

L

K

T

T

I

A

G

G

V

V

M

I

G

G

T

T

G
|
G

R
x
K

I
|
I

G

G

Q

V

I

A

T

A

A

L

Q

R

L

I

F

L

A

K

A

G

F

F

G

G

A

M

K

R

V

L

I

L

G

A

Y
x
F

D
|
D

V
x
P

Y

Y

Q

P

N

S

P

T

N

A

A

A

E

L

N

D

Y

L

-

G

L

V

T

E

Y

Y

I

V

D

D

S

-

F

L

D

Q

E

T

F

L

L

F

A

A

Q

E

S

S

D

D

L

V

L

I

T

S

I

L

H
|
H

M
x
C

P
|
P

L

L

T

T

D

P

D

E

N
|
N

Y

Y

H

H

Q

L

F

L

N

N

T

H

E

A

T

A

F

F

N

D

K

Q

M

M

K

K

P

N

G

G

A

V

I

M

L

I

N

N

P
x
T

A

S

R
|
R

G

G

A

A

I

L

I

I

D

D

T

S

K

Q

D

A

L

A

I

I

A

E

A

A

L

L

D

K

S

N

G

Q

Q

K

I

I

S

G

C

S

C

L

A

G

L

M

D
|
D

T

V

Y
|
Y

E

E

N

N

E
|
E

M

R

P

D

Y

L

V

F

T

F

K

E

D

D

W

K

S

S

G

V

K

D

T

V

L

I

N

Q

D

D

P

D

I

V

L

F

E

R

L

L

I

S

N

A

R

C

E

H

D

N

V

V

L

L

Y

F

T

T

P

G

H
|
H

I

Q

A
|
A

F

F

Y

L

T

T

-

A

-

E

-

A

-

L

-

I

-

S

-

I

-

S

E

E

T

T

A

T

V

L

E

Q

N

N

L

L

active site: S101 (= S97), R234 (= R231), D258 (= D255), E263 (= E260), H295 (= H292)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y96), G153 (= G150), K154 (≠ R151), I155 (= I152), F173 (≠ Y170), D174 (= D171), P175 (≠ V172), H204 (= H201), C205 (≠ M202), P206 (= P203), N211 (= N208), T232 (≠ P229), Y260 (= Y257), H295 (= H292), A297 (= A294)

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
35% identity, 76% coverage: 56:304/327 of query aligns to 52:299/332 of 6biiA

query
sites
6biiA

M

L

Y

S

E

E

K

K

L

I

S

D

Q

R

D

E

G

V

F

F

-

D

-

A

-

P

-

R

-

L

R

R

Q

I

I

V

A

A

Q

N

R

Y

S

A

A
x
V

G

G

V

Y

D

D

M

N

Y

I

D

D

L

I

K

E

E

E

A

A

K

T

K

K

H

R

G

G

I

I

S

Y

I

V

T

T

N

N

V

T

P

P

A

D

Y

V

S
x
L

P

T

N

D

A

A

I
x
T

A

A

E

D

F

L

T

A

V

W

A

A

S

L

A

L

L

L

N

A

C

A

L

A

R

R

H

H

M

V

Q

V

A

K

I

G

Q

D

K

K

R

F

V

V

-

R

-

S

-

G

-

E

-

W

K

K

N

R

H

R

N

G

F

I

S

A

W

W

-

H

D

P

K

K

S

M

I

F

L

L

A

G

R

Y

E

D

V

V

R

Y

S

G

L

K

T

T

I

I

G

G

V

I

M

V

G
|
G

T
x
F

G
|
G

R
|
R

I
|
I

G

G

Q

Q

I

A

T

I

A

A

Q

K

L

R

F

A

A

K

A

G

F

F

G

G

A

M

K

R

V

I

I

L

G

Y

Y

T

D
x
A

V
x
R

Y
x
S

Q

R

N
x
K

P

P

N

E

A

A

E

E

N

K

Y

E

L

L

-

G

T

A

Y

E

I

F

D

K

S

P

F

L

D

E

E

E

F

L

L

L

A

R

Q

E

S

S

D

D

L

F

L

V

T

V

I

L

H

A

M
x
V

P
|
P

L

L

T

T

D

K

D
x
E

N
x
T

Y

Y

H

H

Q

M

F

I

N

N

T

E

E

E

T

R

F

L

N

R

K

L

M

M

K

K

P

P

G

T

A

A

I

V

L

L

L

V

N

N

P
x
V

A
|
A

R
|
R

G

G

A

K

I

V

D

D

T

T

K

K

D

A

L

L

I

I

A

R

A

A

L

L

D

K

S

E

G

G

Q

W

I

I

S

A

C

A

A

G

L

L

D
|
D

T

V

Y

F

E

E

N

E

E
|
E

M

-

P

P

Y

Y

V

Y

T

D

K

-

D

-

W

-

S

-

G

-

K

-

T

-

L

-

N

-

D

-

P

-

I

-

L

-

E

E

L

L

I

F

N

A

R

L

E

D

D

N

V

V

L

V

Y

L

T

T

P

P

H
|
H

I

I

A
x
G

F

S

Y

A

T

T

E

F

T

G

A

A

V

R

E

E

N

G

L

M

active site: L99 (≠ S97), R240 (= R231), D264 (= D255), E269 (= E260), H287 (= H292)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A73), T103 (≠ I101), G156 (= G148), F157 (≠ T149), G158 (= G150), R159 (= R151), I160 (= I152), A179 (≠ D171), R180 (≠ V172), S181 (≠ Y173), K183 (≠ N175), V211 (≠ M202), P212 (= P203), E216 (≠ D207), T217 (≠ N208), V238 (≠ P229), A239 (= A230), R240 (= R231), D264 (= D255), H287 (= H292), G289 (≠ A294)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
34% identity, 76% coverage: 56:304/327 of query aligns to 53:300/334 of 5aovA

query
sites
5aovA

M
x
L

Y

S

E

E

K

R

L

I

S

D

Q

Q

D

E

G

V

F

F

-

E

-

N

-

A

-

P

-

R

-

L

R

R

Q

I

I

V

A

A

Q

N

R
x
Y

S
x
A

A
x
V

G
|
G

V

Y

D

D

M

N

Y

I

D

D

L

V

K

E

E

E

A

A

K

T

K

R

H

R

G

G

I

I

S

Y

I

V

T

T

N

N

V

T

P

P

A

D

Y

V

S
x
L

P

T

N

N

A

A

I
x
T

A

A

E

D

F

H

T

A

V

F

A

A

S

L

A

L

L

L

N

A

C

T

L

A

R

R

H

H

M

V

Q

V

A

K

I

G

Q

D

K

K

R

F

V

V

-

R

-

S

-

G

-

E

-

W

K

K

N

R

H

K

N

G

F

I

S

A

W

W

-

H

D

P

K

K

S

W

I

F

L

L

A

G

R

Y

E

E

V

L

R

Y

S

G

L

K

T

T

I

I

G

G

V

I

M

V

G

G

T
x
F

G
|
G

R
|
R

I
|
I

G

G

Q

Q

I

A

T

I

A

A

Q

R

L

R

F

A

A

K

A

G

F

F

G

N

A

M

K

R

V

I

I

L

G

Y

Y

Y

D
x
S

V
x
R

Y

T

Q

R

N

K

P

S

N

Q

A

A

E

E

N

K

Y

E

L

L

-

G

T

A

Y

E

I

Y

D

R

S

P

F

L

D

E

E

E

F

V

L

L

A

K

Q

E

S

S

D

D

L

F

L

V

T

I

I

L

H
x
A

M
x
V

P
|
P

L

L

T

T

D

K

D

E

N
x
T

Y

M

H

Y

Q

M

F

I

N

N

T

E

E

E

T

R

F

L

N

K

K

L

M

M

K

K

P

P

G

T

A

A

I

I

L

L

L

V

N

N

P
x
I

A
|
A

R
|
R

G

G

A

K

I

V

D

D

T

T

K

K

D

A

L

L

I

I

A

K

A

A

L

L

D

K

S

E

G

G

Q

W

I

I

S

A

C

G

C

A

A

G

L

L

D
|
D

T

V

Y

F

E

E

N

E

E
|
E

M

-

P

P

Y

Y

V

Y

T

N

K

-

D

-

W

-

S

-

G

-

K

-

T

-

L

-

N

-

D

-

P

-

I

-

L

-

E

E

L

L

I

F

N

S

R

L

E

D

D

N

V

V

L

V

Y

L

T

T

P

P

H
|
H

I

I

A
x
G

F

S

Y

A

T

T

E

F

T

E

A

A

V

R

E

E

N

A

L

M

active site: L100 (≠ S97), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H292)
binding glyoxylic acid: L53 (≠ M56), L53 (≠ M56), Y74 (≠ R71), A75 (≠ S72), V76 (≠ A73), G77 (= G74), R241 (= R231), H288 (= H292)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A73), T104 (≠ I101), F158 (≠ T149), G159 (= G150), R160 (= R151), I161 (= I152), S180 (≠ D171), R181 (≠ V172), A211 (≠ H201), V212 (≠ M202), P213 (= P203), T218 (≠ N208), I239 (≠ P229), A240 (= A230), R241 (= R231), H288 (= H292), G290 (≠ A294)

Sites not aligning to the query:

binding glyoxylic acid: 52

4u6sA Ctbp1 in complex with substrate phenylpyruvate (see paper)
32% identity, 81% coverage: 37:300/327 of query aligns to 41:298/328 of 4u6sA

query
sites
4u6sA

L

V

L

L

N

N

G

E

Y

A

D

V

G

G

L

A

S

L

L

M

Q
x
Y

Q
x
H

T

T

M

I

G

T

I

L

P

T

A

R

E

E

M

D

Y

L

E

E

K

K

L

F

S

K

Q

-

D

-

G

A

F

L

R

R

Q

I

I

I

A

V

Q

R

R
x
I

S
x
G

A
x
S

G
|
G

V

F

D

D

M

N

Y

I

D

D

L

I

K

K

E

S

A

A

K

G

K

D

H

L

G

G

I

I

S

A

I

V

T

C

N

N

V

V

P

P

A

A

Y

A

S
|
S

P

V

N

E

A

E

I
x
T

A

A

E

D

F

S

T

T

V

L

A

C

S

H

A

I

L

L

N

N

C

L

L

Y

R

R

H

R

M

A

-

T

-

W

-

L

-

H

Q

Q

A

A

I

L

Q

R

K

E

R

G

V

T

K

R

N

V

H

Q

N

S

F

V

S

E

W

Q

D

I

K

R

S

E

I

V

L

A

-

S

-

G

A

A

R

A

E

R

V

I

R

R

S

G

L

E

T

T

I

L

G

G

V

I

M

I

G
|
G

T

L

G
|
G

R
|
R

I
x
V

G

G

Q

Q

I

A

T

V

A

A

Q

L

L

R

F

A

A

K

A

A

F

F

G

G

A

F

K

N

V

V

I

L

G

F

Y
|
Y

D
|
D

V
x
P

Y
|
Y

Q

L

N

S

P

D

N

G

A

V

E

E

N

R

Y

A

L

L

-

G

-

L

T

Q

Y

R

I

V

D

S

S

T

F

L

D

Q

E

D

F

L

L

L

A

F

Q

H

S

S

D

D

L

C

L

V

T

T

I

L

H
|
H

M
x
C

P
x
G

L

L

T

N

D

E

D

H

N
|
N

Y

H

H

H

Q

L

F

I

N

N

T

D

E

F

T

T

F

V

N

K

K

Q

M

M

K

R

P

Q

G

G

A

A

I

F

L

L

L

V

N

N

P
x
T

A
|
A

R
|
R

G

G

A

G

I

L

I

V

D

D

T

E

K

K

D

A

L

L

I

A

A

Q

A

A

L

L

D

K

S

E

G

G

Q

R

I

I

S

R

C

G

C

A

A

A

L

L

D
|
D

T

V

Y

H

E

E

N

S

E
|
E

M

-

P

P

Y

F

V

-

T

-

K

-

D

S

W

F

S

S

G

Q

K

G

T

P

L

L

N

K

D

D

P

A

I

-

L

-

E

-

L

-

I

-

N

-

R

-

E

P

D

N

V

L

L

I

Y

C

T

T

P

P

H
|
H

I

A

A

A

F
x
W

Y

Y

T

S

E

E

T

Q

A

A

active site: S99 (= S97), R241 (= R231), D265 (= D255), E270 (= E260), H290 (= H292)
binding nicotinamide-adenine-dinucleotide: T103 (≠ I101), G156 (= G148), G158 (= G150), R159 (= R151), V160 (≠ I152), Y178 (= Y170), D179 (= D171), P180 (≠ V172), Y181 (= Y173), H211 (= H201), C212 (≠ M202), G213 (≠ P203), N218 (= N208), T239 (≠ P229), A240 (= A230), R241 (= R231), H290 (= H292), W293 (≠ F295)
binding 3-phenylpyruvic acid: Y51 (≠ Q47), H52 (≠ Q48), I73 (≠ R71), G74 (≠ S72), S75 (≠ A73), G76 (= G74), R241 (= R231), W293 (≠ F295)

Sites not aligning to the query:

binding 3-phenylpyruvic acid: 302

4u6qA Ctbp1 bound to inhibitor 2-(hydroxyimino)-3-phenylpropanoic acid (see paper)
32% identity, 81% coverage: 37:300/327 of query aligns to 41:298/328 of 4u6qA

query
sites
4u6qA

L

V

L

L

N

N

G

E

Y

A

D

V

G

G

L

A

S

L

L

M

Q
x
Y

Q

H

T

T

M

I

G

T

I

L

P

T

A

R

E

E

M

D

Y

L

E

E

K

K

L

F

S

K

Q

-

D

-

G

A

F

L

R

R

Q

I

I

I

A

V

Q

R

R
x
I

S
x
G

A
x
S

G
|
G

V

F

D

D

M

N

Y

I

D

D

L

I

K

K

E

S

A

A

K

G

K

D

H

L

G

G

I

I

S

A

I

V

T

C

N

N

V

V

P

P

A

A

Y

A

S
|
S

P

V

N

E

A

E

I
x
T

A

A

E

D

F

S

T

T

V

L

A

C

S

H

A

I

L

L

N

N

C

L

L

Y

R

R

H

R

M

A

-

T

-

W

-

L

-

H

Q

Q

A

A

I

L

Q

R

K

E

R

G

V

T

K

R

N

V

H

Q

N

S

F

V

S

E

W

Q

D

I

K

R

S

E

I

V

L

A

-

S

-

G

A

A

R

A

E

R

V

I

R

R

S

G

L

E

T

T

I

L

G

G

V

I

M

I

G
|
G

T

L

G

G

R
|
R

I
x
V

G

G

Q

Q

I

A

T

V

A

A

Q

L

L

R

F

A

A

K

A

A

F

F

G

G

A

F

K

N

V

V

I

L

G

F

Y
|
Y

D
|
D

V
x
P

Y
|
Y

Q

L

N

S

P

D

N

G

A

V

E

E

N

R

Y

A

L

L

-

G

-

L

T

Q

Y

R

I

V

D

S

S

T

F

L

D

Q

E

D

F

L

L

L

A

F

Q

H

S

S

D

D

L

C

L

V

T

T

I

L

H
|
H

M
x
C

P
x
G

L

L

T

N

D

E

D

H

N
|
N

Y

H

H

H

Q

L

F

I

N

N

T

D

E

F

T

T

F

V

N

K

K

Q

M

M

K

R

P

Q

G

G

A

A

I

F

L

L

L

V

N

N

P
x
T

A
|
A

R
|
R

G

G

A

G

I

L

I

V

D

D

T

E

K

K

D

A

L

L

I

A

A

Q

A

A

L

L

D

K

S

E

G

G

Q

R

I

I

S

R

C

G

C

A

A

A

L

L

D
|
D

T

V

Y

H

E

E

N

S

E
|
E

M

-

P

P

Y

F

V

-

T

-

K

-

D

S

W

F

S

S

G

Q

K

G

T

P

L

L

N

K

D

D

P

A

I

-

L

-

E

-

L

-

I

-

N

-

R

-

E

P

D

N

V

L

L

I

Y

C

T

T

P

P

H
|
H

I

A

A

A

F
x
W

Y

Y

T

S

E

E

T

Q

A

A

active site: S99 (= S97), R241 (= R231), D265 (= D255), E270 (= E260), H290 (= H292)
binding (2E)-2-(hydroxyimino)-3-phenylpropanoic acid: Y51 (≠ Q47), I73 (≠ R71), G74 (≠ S72), S75 (≠ A73), G76 (= G74), R241 (= R231), H290 (= H292), W293 (≠ F295)
binding 1,4-dihydronicotinamide adenine dinucleotide: S75 (≠ A73), T103 (≠ I101), G156 (= G148), R159 (= R151), V160 (≠ I152), Y178 (= Y170), D179 (= D171), P180 (≠ V172), Y181 (= Y173), H211 (= H201), C212 (≠ M202), G213 (≠ P203), N218 (= N208), T239 (≠ P229), A240 (= A230), R241 (= R231), H290 (= H292), W293 (≠ F295)

Sites not aligning to the query:

binding (2E)-2-(hydroxyimino)-3-phenylpropanoic acid: 302

6cdfA Human ctbp1 (28-378) (see paper)
32% identity, 81% coverage: 37:300/327 of query aligns to 42:299/333 of 6cdfA

query
sites
6cdfA

L

V

L

L

N

N

G

E

Y

A

D

V

G

G

L

A

S

L

L

M

Q

Y

Q

H

T

T

M

I

G

T

I

L

P

T

A

R

E

E

M

D

Y

L

E

E

K

K

L

F

S

K

Q

-

D

-

G

A

F

L

R

R

Q

I

I

I

A

V

Q

R

R

I

S

G

A

S

G

G

V

F

D

D

M

N

Y

I

D

D

L

I

K

K

E

S

A

A

K

G

K

D

H

L

G

G

I

I

S

A

I

V

T

C

N

N

V

V

P

P

A

A

Y

A

S

S

P

V

N

E

A

E

I
x
T

A

A

E

D

F

S

T

T

V

L

A

C

S

H

A

I

L

L

N

N

C

L

L

Y

R

R

H

R

M

A

-

T

-

W

-

L

-

H

Q

Q

A

A

I

L

Q

R

K

E

R

G

V

T

K

R

N

V

H

Q

N

S

F

V

S

E

W

Q

D

I

K

R

S

E

I

V

L

A

-

S

-

G

A

A

R

A

E

R

V

I

R

R

S

G

L

E

T

T

I

L

G

G

V

I

M

I

G
|
G

T

L

G

G

R
|
R

I
x
V

G

G

Q

Q

I

A

T

V

A

A

Q

L

L

R

F

A

A

K

A

A

F

F

G

G

A

F

K

N

V

V

I

L

G

F

Y
|
Y

D
|
D

V
x
P

Y
|
Y

Q

L

N

S

P

D

N

G

A

V

E

E

N

R

Y

A

L

L

-

G

-

L

T

Q

Y

R

I

V

D

S

S

T

F

L

D

Q

E

D

F

L

L

L

A

F

Q

H

S

S

D

D

L

C

L

V

T

T

I

L

H
|
H

M
x
C

P

G

L

L

T

N

D

E

D

H

N
|
N

Y

H

H

H

Q

L

F

I

N

N

T

D

E

F

T

T

F

V

N

K

K

Q

M

M

K

R

P

Q

G

G

A

A

I

F

L

L

L

V

N

N

P
x
T

A
|
A

R
|
R

G

G

A

G

I

L

I

V

D

D

T

E

K

K

D

A

L

L

I

A

A

Q

A

A

L

L

D

K

S

E

G

G

Q

R

I

I

S

R

C

G

C

A

A

A

L

L

D

D

T

V

Y

H

E

E

N

S

E

E

M

-

P

P

Y

F

V

-

T

-

K

-

D

S

W

F

S

S

G

Q

K

G

T

P

L

L

N

K

D

D

P

A

I

-

L

-

E

-

L

-

I

-

N

-

R

-

E

P

D

N

V

L

L

I

Y

C

T

T

P

P

H
|
H

I

A

A

A

F
x
W

Y

Y

T

S

E

E

T

Q

A

A

binding 1,4-dihydronicotinamide adenine dinucleotide: T104 (≠ I101), G157 (= G148), R160 (= R151), V161 (≠ I152), Y179 (= Y170), D180 (= D171), P181 (≠ V172), Y182 (= Y173), H212 (= H201), C213 (≠ M202), N219 (= N208), T240 (≠ P229), A241 (= A230), R242 (= R231), H291 (= H292), W294 (≠ F295)

Query Sequence

>WP_077276850.1 NCBI__GCF_001998885.1:WP_077276850.1
MMGAREDERRFAEKWAKDHNVTIEVSTDILSDDTYHLLNGYDGLSLQQTMGIPAEMYEKL
SQDGFRQIAQRSAGVDMYDLKEAKKHGISITNVPAYSPNAIAEFTVASALNCLRHMQAIQ
KRVKNHNFSWDKSILAREVRSLTIGVMGTGRIGQITAQLFAAFGAKVIGYDVYQNPNAEN
YLTYIDSFDEFLAQSDLLTIHMPLTDDNYHQFNTETFNKMKPGAILLNPARGAIIDTKDL
IAALDSGQISCCALDTYENEMPYVTKDWSGKTLNDPILEELINREDVLYTPHIAFYTETA
VENLVFGGLDACLSILTNGTADTIVNP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory