SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_077278677.1 NCBI__GCF_002000365.1:WP_077278677.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
30% identity, 11% coverage: 172:313/1288 of query aligns to 205:341/658 of O00116

query
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O00116

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Sites not aligning to the query:

182 R → Q: in RCDP3; severely reduced protein levels
419 Important for enzyme activity; R → H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411
471 E → K: in RCDP3; severely reduced protein levels
568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214

5adzA Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 113:249/566 of 5adzA

query
sites
5adzA

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binding flavin-adenine dinucleotide: P142 (= P201), I143 (≠ R202), G144 (= G203), G145 (= G204), G146 (= G205), T147 (= T206), S148 (≠ G207), G152 (= G210), P210 (= P274), D211 (≠ T275), S212 (= S276), S216 (= S280), T217 (= T281), G220 (= G284), W221 (≠ N285), S223 (≠ A287), T224 (≠ M288), A226 (= A290), S227 (≠ G291)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D211 (≠ T275)

Sites not aligning to the query:

5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 111:247/539 of 5ae3A

query
sites
5ae3A

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binding flavin-adenine dinucleotide: P140 (= P201), I141 (≠ R202), G142 (= G203), G143 (= G204), G144 (= G205), T145 (= T206), S146 (≠ G207), V147 (≠ Y208), L151 (≠ A211), P208 (= P274), D209 (≠ T275), S210 (= S276), S214 (= S280), T215 (= T281), G218 (= G284), W219 (≠ N285), S221 (≠ A287), T222 (≠ M288), A224 (= A290), S225 (≠ G291)

Sites not aligning to the query:

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 116:252/561 of 5ae2A

query
sites
5ae2A

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binding flavin-adenine dinucleotide: P145 (= P201), I146 (≠ R202), G147 (= G203), G148 (= G204), G149 (= G205), T150 (= T206), S151 (≠ G207), G155 (= G210), L156 (≠ A211), P213 (= P274), D214 (≠ T275), S215 (= S276), S219 (= S280), T220 (= T281), G223 (= G284), W224 (≠ N285), S226 (≠ A287), T227 (≠ M288), A229 (= A290), S230 (≠ G291)

Sites not aligning to the query:

5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 125:261/557 of 5adzC

query
sites
5adzC

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binding flavin-adenine dinucleotide: P154 (= P201), I155 (≠ R202), G156 (= G203), G157 (= G204), G158 (= G205), T159 (= T206), S160 (≠ G207), P222 (= P274), D223 (≠ T275), S224 (= S276), S228 (= S280), T229 (= T281), G232 (= G284), W233 (≠ N285), S235 (≠ A287), T236 (≠ M288), A238 (= A290), S239 (≠ G291)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ T275)

Sites not aligning to the query:

6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 125:261/554 of 6gouA

query
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6gouA

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binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ T275)
binding flavin-adenine dinucleotide: P154 (= P201), I155 (≠ R202), G156 (= G203), G157 (= G204), G158 (= G205), T159 (= T206), S160 (≠ G207), V161 (≠ Y208), G164 (= G210), P222 (= P274), D223 (≠ T275), S224 (= S276), S228 (= S280), T229 (= T281), G232 (= G284), W233 (≠ N285), S235 (≠ A287), T236 (≠ M288), A238 (= A290), S239 (≠ G291)

Sites not aligning to the query:

5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 110:246/560 of 5ae1A

query
sites
5ae1A

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binding flavin-adenine dinucleotide: P139 (= P201), I140 (≠ R202), G141 (= G203), G142 (= G204), G143 (= G205), T144 (= T206), S145 (≠ G207), V146 (≠ Y208), G149 (= G210), L150 (≠ A211), P207 (= P274), D208 (≠ T275), S209 (= S276), S213 (= S280), T214 (= T281), G216 (= G283), G217 (= G284), W218 (≠ N285), S220 (≠ A287), T221 (≠ M288), A223 (= A290), S224 (≠ G291)

Sites not aligning to the query:

4bc7B Mammalian alkyldihydroxyacetonephosphate synthase: arg419his mutant (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 111:247/543 of 4bc7B

query
sites
4bc7B

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W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R

I

G
|
G

T
|
T

G
x
S

Y
x
V

T

S

-

Y

G
|
G

A
x
L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V

H

D

E

P
|
P

T
x
D

S
|
S

Q

L

D

E

A

F

S
|
S

T
|
T

I

V

G

G

G
|
G

N

W

I

I

A
x
S

M
x
T

N

R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

binding flavin-adenine dinucleotide: P140 (= P201), G142 (= G203), G143 (= G204), G144 (= G205), T145 (= T206), S146 (≠ G207), V147 (≠ Y208), G150 (= G210), L151 (≠ A211), P208 (= P274), D209 (≠ T275), S210 (= S276), S214 (= S280), T215 (= T281), G218 (= G284), S221 (≠ A287), T222 (≠ M288), A224 (= A290), S225 (≠ G291)

Sites not aligning to the query:

4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 116:252/555 of 4bc9A

query
sites
4bc9A

L

I

P

P

F

D

V

I

V

V

V

L

T

W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R
x
I

G
|
G

T
|
T

G
x
S

Y
x
V

T

S

-

Y

G
|
G

A
x
L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V

H

D

E

P
|
P

T
x
D

S
|
S

Q

L

D

E

A

F

S
|
S

T
|
T

I

V

G

G

G
|
G

N
x
W

I

I

A
x
S

M
x
T

N

R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

binding propanenitrile: D214 (≠ T275)
binding flavin-adenine dinucleotide: P145 (= P201), I146 (≠ R202), G147 (= G203), G148 (= G204), G149 (= G205), T150 (= T206), S151 (≠ G207), V152 (≠ Y208), G155 (= G210), L156 (≠ A211), P213 (= P274), D214 (≠ T275), S215 (= S276), S219 (= S280), T220 (= T281), G223 (= G284), W224 (≠ N285), S226 (≠ A287), T227 (≠ M288), A229 (= A290), S230 (≠ G291)

Sites not aligning to the query:

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 111:247/542 of 5ae2B

query
sites
5ae2B

L

I

P

P

F

D

V

I

V

V

V

L

T

W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R

I

G
|
G

T
|
T

G
x
S

Y

V

T

S

-

Y

G
|
G

A
x
L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V

H

D

E

P
|
P

T
x
D

S
|
S

Q

L

D

E

A

F

S
|
S

T
|
T

I

V

G

G

G
|
G

N
x
W

I

I

A
x
S

M
x
T

N

R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

binding flavin-adenine dinucleotide: P140 (= P201), G142 (= G203), G143 (= G204), G144 (= G205), T145 (= T206), S146 (≠ G207), G150 (= G210), L151 (≠ A211), P208 (= P274), D209 (≠ T275), S210 (= S276), S214 (= S280), T215 (= T281), G218 (= G284), W219 (≠ N285), S221 (≠ A287), T222 (≠ M288), A224 (= A290), S225 (≠ G291)

Sites not aligning to the query:

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 111:247/542 of 4bc9B

query
sites
4bc9B

L

I

P

P

F

D

V

I

V

V

V

L

T

W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R

I

G
|
G

T
|
T

G
x
S

Y
x
V

T

S

-

Y

G
|
G

A
x
L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V

H

D

E

P
|
P

T
x
D

S
|
S

Q

L

D

E

A

F

S
|
S

T
|
T

I

V

G

G

G
|
G

N
x
W

I

I

A
x
S

M
x
T

N

R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

binding propanenitrile: D209 (≠ T275)
binding flavin-adenine dinucleotide: P140 (= P201), G142 (= G203), G143 (= G204), G144 (= G205), T145 (= T206), S146 (≠ G207), V147 (≠ Y208), G150 (= G210), L151 (≠ A211), P208 (= P274), D209 (≠ T275), S210 (= S276), S214 (= S280), T215 (= T281), G218 (= G284), W219 (≠ N285), S221 (≠ A287), T222 (≠ M288), A224 (= A290), S225 (≠ G291)

Sites not aligning to the query:

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
30% identity, 11% coverage: 172:313/1288 of query aligns to 205:341/658 of P97275

query
sites
P97275

L

I

P

P

F

D

V

I

V

V

V

L

T

W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R
x
I

G
|
G

T
|
T

G
x
S

Y

V

T

S

-

Y

G

G

A

L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V
x
H

D

E

P

P

T
x
D

S
|
S

Q
x
L

D
x
E

A
x
F

S
|
S

T
|
T

I

V

G

G

N

W

I

I

A

S

M
x
T

N
x
R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

234:240 (vs. 201:207, 71% identical) binding FAD
H300 (≠ V272) mutation to A: Loss of activity.
303:309 (vs. 275:281, 43% identical) binding FAD
T309 (= T281) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
TRAS 316:319 (≠ MNAG 288:291) binding FAD

Sites not aligning to the query:

1:58 modified: transit peptide, Peroxisome
367 S→A: Strongly reduced activity.
368:374 binding FAD
419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
469 L→P: Impaired FAD binding and protein stability. Loss of activity.
515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
576 C→A: No effect on activity.
578 active site, Proton donor/acceptor; Y→F: Loss of activity.
615 H→A: Loss of activity.
615:617 Important for enzyme activity
616 H→A: Loss of activity.
617 H→A: Loss of activity.
654:658 Important for enzyme activity

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
30% identity, 11% coverage: 172:313/1288 of query aligns to 111:247/539 of 5ae1B

query
sites
5ae1B

L

I

P

P

F

D

V

I

V

V

V

L

T

W

P

P

D

T

S

C

E

H

A

D

E

D

M

V

A

V

G

K

V

I

V

V

A

N

A

L

C

A

I

C

E

K

L

Y

G

N

L

L

T

C

V

I

I

I

P
|
P

R
x
I

G
|
G

T
|
T

G
x
S

Y

V

T

S

-

Y

G
|
G

A
x
L

A

M

V

C

P

P

L

A

D

D

E

E

R

T

S

R

A

T

V

I

I

I

N

S

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

D

S

T

V

S

E

Q

M

M

R

N

R

R

I

I

P

L

G

W

V

V

E

D

E

E

S

N

V

N

P

L

T

T

V

A

R

H

A

V

E

E

A

A

G

G

V

I

V

T

T

G

R

Q

R

E

V

L

S

E

E

R

R

Q

A

L

A

K

E

E

E

S

G

G

L

Y

V

C

F

T

A

G

V

H

D

E

P
|
P

T
x
D

S
|
S

Q

L

D

E

A

F

S
|
S

T
|
T

I

V

G

G

G
|
G

N
x
W

I

I

A
x
S

M
x
T

N

R

A
|
A

G
x
S

G

G

K

M

K

K

A

K

V

N

L

I

W

Y

G

G

T

N

T

I

I

E

D

D

N

L

L

V

A

V

S

H

W

M

R

K

M

V

V

V

T

T

P

P

binding flavin-adenine dinucleotide: P140 (= P201), I141 (≠ R202), G142 (= G203), G143 (= G204), G144 (= G205), T145 (= T206), S146 (≠ G207), G150 (= G210), L151 (≠ A211), P208 (= P274), D209 (≠ T275), S210 (= S276), S214 (= S280), T215 (= T281), G218 (= G284), W219 (≠ N285), S221 (≠ A287), T222 (≠ M288), A224 (= A290), S225 (≠ G291)

Sites not aligning to the query:

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
24% identity, 29% coverage: 175:548/1288 of query aligns to 43:357/465 of 3pm9A

query
sites
3pm9A

V

L

V

V

V

L

T

R

P

P

D

G

S

S

E

T

A

E

E

E

M

V

A

V

G

A

V

I

V

C

A

K

A

L

C

A

I

N

E

E

L

A

G

R

L

V

T

A

V

L

I

V

P
|
P

R
x
Q

G
|
G

G
x
N

T
|
T

G
|
G

Y
x
L

T

V

G

G

A
x
G

A
x
Q

V

T

P

P

L

H

D

N

E

G

R

E

S

-

A

V

V

V

I

I

N

S

T
x
L

E

K

K

R

L

-

E

-

G

-

L

-

G

-

S

-

V

-

E

-

M

M

R

D

R

K

I

I

P

R

G

E

V

I

E

D

E

T

S

S

V

S

P

N

T

T

V

I

R

T

A

V

E

E

A

A

G

G

V

A

V

I

T

L

R

Q

R

R

V

V

S

Q

E

E

R

K

A

A

E

E

E

V

G

D

L

R

V

L

F

F

A

P

V

L

D

S

P
x
L

T
x
G

S
x
A

Q

Q

D

G

A

S

S
x
C

T
|
T

I

I

G
|
G

N

N

I

L

A
x
S

M
x
T

N

N

A
|
A

G
|
G

G

G

K

T

K

A

A

A

V

L

L

A

W

Y

G

G

T
x
L

T

A

I

R

D

D

N

M

L

A

A

L

S

G

W

V

R

E

M

V

V

V

T

L

P

A

D

D

A

G

K

-

W

-

L

-

E

-

V

-

E

-

R

R

V

V

G

M

H

N

N

L

L

L

G

S

K

K

I

L

H

K

D

K

Q

D

E

N

T

T

V

-

R

-

F

-

R

-

I

-

T

-

R

-

Y

-

S

-

A

-

D

-

G

-

V

-

T

-

P

-

E

-

G

-

A

-

P

-

E

-

I

-

S

-

A

-

P

-

G

-

Q

-

S

-

F

-

R

-

K

-

L

-

G

-

L

-

G

G

K

Y

D

D

V

L

T

R

D

D

K

L

H

F

L

I

G

G

G

A

L

-

P

-

G

-

V

-

Q

-

K

-

E
|
E

G
|
G

C

T

D

L

G

G

L
x
I

I
|
I

T

T

S

A

A

A

V

T

F

L

V

K

L

L

H

F

R

P

M

K

P

P

K

R

H

A

I

V

R

E

T

T

V

A

C

F

L

V

E

G

F

L

Y

Q

G

S

D

P

D

D

L

D

H

A

E

L

A

K

V

L

P

L

A

G

I

I

V

A

E

Q

I

-

K

-

D

-

R

-

L

-

D

-

G

G

D

E

P

A

Q

A

V

G

R

N

L

L

S

T

G

S

L

F

E

E

H

L

L

I

D

A

E

E

R

T

Y

P

V

L

R

D

-

F

A

S

V

V

R

R

Y

H

T

A

-

N

-

R

-

D

P

P

K

L

G

E

A

A

R

R

G

-

E

-

R

Y

P

P

K

W

M

Y

V

V

L

L

L

I

A

-

D

E

V

L

C

S

S

S

D

P

D

R

E

D

D

D

A

A

V

-

G

-

R

R

A

A

S

L

A

E

M

S

V

I

R

L

L

E

A

R

N

G

A

F

R

E

N

D

G

G

-

I

-

V

E

D

G

A

F

A

V

I

A

A

V

N

S

S

A

V

E

Q

A

Q

R

Q

K

Q

R

A

F

F

W

W

S

K

E

L

R

R

A

E

R

E

T

I

A

S

A

P

I

-

A

A

A

Q

H

K

T

P

N

E

A

G

F

G

K

S

I

I

N

K

E

H

D

D

V

I

V

S

I

V

P

P

L

V

D

A

R

A

L

V

A

P

E

Q

Y

F

S

I

E

E

active site: A149 (= A290), L159 (≠ T300)
binding flavin-adenine dinucleotide: P69 (= P201), Q70 (≠ R202), G71 (= G203), G72 (= G204), N73 (≠ G205), T74 (= T206), G75 (= G207), L76 (≠ Y208), G79 (≠ A211), Q80 (≠ A212), L91 (≠ T224), L133 (≠ P274), G134 (≠ T275), A135 (≠ S276), C139 (≠ S280), T140 (= T281), G142 (= G283), G143 (= G284), S146 (≠ A287), T147 (≠ M288), A149 (= A290), G150 (= G291), E200 (= E387), G201 (= G388), I205 (≠ L392), I206 (= I393)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 423

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
24% identity, 29% coverage: 173:548/1288 of query aligns to 45:359/467 of 7qh2C

query
sites
7qh2C

P

P

F

E

V

V

V

L

V

I

T

K

P

V

D

T

S

S

E

T

A

E

E

E

M

V

A

S

G

K

V

I

V

M

A

K

A

Y

C

A

I

Y

E

E

L

H

G

N

L

I

T

P

V

V

I

V

P
x
V

R

R

G
|
G

G
x
S

G
|
G

T
|
T

G
|
G

Y
x
L

T

V

G

G

A
|
A

A
x
C

V

V

P

P

L

L

D

-

E

-

R

F

S

G

A

G

V

I

I

M

N

-

T

-

E

-

K

-

L

-

E

-

G

-

L

L

G

E

S

T

V

T

E

L

M

M

R

N

I

I

P

L

G

E

V

L

E

D

E

T

S

E

V

N

P

L

T

T

V

V

R

T

A

V

E

E

A

P

G

G

V

V

V

L

T

L

R

M

R

E

V

L

S

S

E

K

R

F

A

V

A

E

E

E

E

N

G

D

L

L

V

F

F

Y

A

P

V

P

D

D

P
|
P

T
x
G

S
x
E

Q

K

D

S

A
|
A

S

-

T
|
T

I

I

G

A

G
|
G

N
|
N

I

I

A
x
S

M
x
T

N

N

A
|
A

G
|
G

G

G

K

M

K

R

A

A

V

V

L

K

W

Y

G

G

T

V

T

T

I

R

D

D

N

Y

L

V

A

R

S

G

W

L

R

T

M

V

V

V

T

L

P

A

D

N

A

G

K

E

W

I

L

I

E

E

V

L

E

G

R

-

V

-

G

-

H

-

N

-

L

-

G

G

K

K

I

I

H

V

D

K

Q

N

E

S

T

S

V

-

R

-

F

-

R

-

I

-

T

-

R

-

Y

-

S

-

A

-

D

-

G

-

V

-

T

-

P

-

E

-

G

-

A

-

P

-

E

-

I

-

S

-

A

-

P

-

G

G

Q

Y

S

S

F

L

R

K

K

D

L

L

G

V

L

I

G

G

K

-

D

-

V

-

T

-

D

-

K

-

H

-

L

-

G

-

L

-

P

-

G

-

V

-

Q

-

K

S

E
|
E

G
|
G

C

T

D

L

G

C

L

V

I
|
I

T

T

S

K

A

A

V

I

F

L

V

K

L

L

H

L

R

P

M

L

P

P

K

K

H

M

I

T

R

L

T

S

V

L

C

L

L

I

E

P

F

F

Y

E

G

N

-

I

D

S

D

D

L

A

H

A

E

G

A

I

V

V

P

P

A

K

I

I

V

I

E

K

I

S

K

K

D

-

R

-

L

-

D

-

G

A

D

I

P

P

Q

-

V

-

R

-

L

-

S

T

G

A

L

I

E

E

H

F

L

M

D

E

E

R

R

Q

Y

T

V

I

R

L

-

F

A

A

V

E

R

D

Y

F

T

L

P

G

K

K

G

K

A

F

R

P

G

D

E

S

R

S

P

S

K

N

M

A

V

Y

L

I

L

L

A

L

D

T

V

F

C

D

S

G

D

N

D

T

E

K

D

E

A

Q

V

V

G

E

R

A

A

E

A

Y

S

E

A

T

M

V

V

A

R

N

L

L

A

C

N

L

A

A

R

E

N

G

G

A

E

K

G

D

-

V

F

Y

V

I

A

V

V

D

S

T

A

V

E

E

A

R

R

K

K

D

R

S

F

V

W

W

S

S

E

A

R

R

-

G

A

A

R

F

T

L

A

E

A

A

I

I

A

K

A

A

H

S

T

T

N

T

A

-

F

-

K

E

I

M

N

D

E

E

-

C

D

D

V

V

I

V

P

P

L

R

D

N

R

R

L

I

A

A

E

E

Y

F

S

I

E

E

binding flavin-adenine dinucleotide: V73 (≠ P201), G75 (= G203), S76 (≠ G204), G77 (= G205), T78 (= T206), G79 (= G207), L80 (≠ Y208), A83 (= A211), C84 (≠ A212), P137 (= P274), G138 (≠ T275), E139 (≠ S276), A142 (= A279), T143 (= T281), G146 (= G284), N147 (= N285), S149 (≠ A287), T150 (≠ M288), A152 (= A290), G153 (= G291), E203 (= E387), G204 (= G388), I209 (= I393)

Sites not aligning to the query:

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
25% identity, 30% coverage: 158:545/1288 of query aligns to 25:348/455 of 8jduA

query
sites
8jduA

R

R

V

E

S

Q

H

H

V

G

T

H

D

D

A

E

T

S

D

M

W

H

R

R

V

C

E

Q

L

P

P

P

F

D

V

A

V

V

T

W

P

P

D

Q

S

N

E

V

A

D

E

Q

M

V

A

S

G

R

V

V

V

A

A

S

A

L

C

C

I

Y

E

N

L

Q

G

G

L

V

T

P

V

I

I

I

P
|
P

R

F

G
|
G

G
x
T

G
|
G

T
|
T

G
|
G

Y

V

T

E

G

G

A
x
G

A
x
V

V

C

P

A

L

V

D

-

E

Q

R

G

S

G

A

V

V

C

I

I

N

N

T
x
L

E

T

K

H

L

M

E

D

G

Q

L

I

G

T

S

E

V

L

E

N

M

T

R

E

R

D

I

F

P

-

G

-

V

-

E

-

S

-

V

-

P

-

T

S

V

V

R

V

A

V

E

E

A

P

G

G

V

V

V

T

T

R

R

K

R

A

V

L

S

N

E

T

R

H

A

L

A

R

E

D

E

S

G

G

L

L

V

W

F

F

A

P

V

V

D

D

P
|
P

T
x
G

S
x
A

Q

-

D

D

A

A

S

S

T

-

I

L

G

C

G
|
G

N
x
M

I

A

A
|
A

M
x
T

N

G

A
|
A

G
x
S

G

G

K

T

K

N

A

A

V

V

L

R

W

Y

G

G

T

T

T

M

I

R

D

D

N

N

L

V

A

I

S

N

W

L

R

E

M

V

V

V

T

L

P

P

D

D

A

G

K

R

W

L

L

L

E

-

V

-

E

-

R

-

V

-

G

-

H

H

N

T

L

A

G

G

K

R

I

-

H

-

D

-

Q

-

E

-

T

-

V

-

R

-

F

-

R

-

I

-

T

-

R

-

Y

-

S

-

A

-

D

-

G

-

V

-

T

-

P

-

E

-

G

-

A

-

P

-

E

-

I

-

S

-

A

-

P

-

G

G

Q

R

S

H

F

Y

R

R

K

K

L

S

G

A

L

A

G

G

K

Y

D

N

V

L

T

T

D

G

K

L

H

F

L

V

G

G

G

-

L

-

P

-

G

-

V

-

Q

-

K

S

E
|
E

G
|
G

C

T

D

L

G

G

L

I

I
|
I

T

T

S

S

A

T

V

T

F

L

V

R

L

L

H

H

R

P

M

A

P

P

K

E

H

A

I

T

R

V

T

A

V

A

C

T

L

C

E

A

F

F

Y

-

G

-

D

P

D

S

L

V

H

Q

E

A

A

A

V

V

P

D

A

S

I

T

V

V

E

Q

I

I

K

L

D

-

R

-

L

-

D

-

G

-

D

Q

P

A

Q

A

V

V

R

P

L

V

S

A

G

R

L

I

E

E

H

F

L

L

D

D

E

D

R

V

Y

M

V

M

R

D

A

A

V

C

R

N

Y

R

T

H

P

S

K

K

G

L

A

N

R

C

G

P

E

V

R

A

P

P

K

T

M

L

V

F

L

L

L

-

A

-

D

-

V

-

C

-

S

-

D

E

D

F

E

H

D

G

A

S

V

Q

G

Q

R

T

A

L

A

A

S

E

A

Q

M

L

V

Q

R

R

L

T

-

E

A

A

N

I

A

T

R

Q

N

N

G

G

E

G

G

S

F

H

V

F

A

S

V

W

S

A

A

A

E

E

A

K

R

R

K

N

R

E

F

L

W

W

S

A

E

A

R
|
R

-

H

-

N

A

A

R
x
W

T

Y

A

A

I

L

A

A

A

L

H

S

T

P

N

G

A

S

F

K

K

A

I

Y

N

S

E

T

D

D

V

V

V

C

I

V

P

P

L

I

D

S

R

R

L

L

A

P

E

E

binding 2-oxopentanoic acid: R318 (= R517), W322 (≠ R519)
binding flavin-adenine dinucleotide: P68 (= P201), G70 (= G203), T71 (≠ G204), G72 (= G205), T73 (= T206), G74 (= G207), G78 (≠ A211), V79 (≠ A212), L90 (≠ T224), P132 (= P274), G133 (≠ T275), A134 (≠ S276), G140 (= G284), M141 (≠ N285), A143 (= A287), T144 (≠ M288), A146 (= A290), S147 (≠ G291), E200 (= E387), G201 (= G388), I206 (= I393), W322 (≠ R519)

Sites not aligning to the query:

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
25% identity, 30% coverage: 158:545/1288 of query aligns to 25:347/454 of 8jdvA

query
sites
8jdvA

R

R

V

E

S

Q

H

H

V

G

T

H

D

D

A

E

T

S

D

M

W

H

R

R

V

C

E

Q

L

P

P

P

F

D

V

A

V

V

T

W

P

P

D

Q

S

N

E

V

A

D

E

Q

M

V

A

S

G

R

V

V

V

A

A

S

A

L

C

C

I

Y

E

N

L

Q

G

G

L

V

T

P

V

I

I

I

P
|
P

R

F

G
|
G

G
x
T

G
|
G

T
|
T

G
|
G

Y
x
V

T

E

G

G

A
x
G

A
x
V

V

C

P

A

L

V

D

-

E

Q

R

G

S

G

A

V

V

C

I

I

N

N

T
x
L

E

T

K

H

L

M

E

D

G

Q

L

I

G

T

S

E

V

L

E

N

M

T

R

E

R

D

I

F

P

-

G

-

V

-

E

-

S

-

V

-

P

-

T

S

V

V

R

V

A

V

E

E

A

P

G

G

V

V

V

T

T

R

R

K

R

A

V

L

S

N

E

T

R

H

A

L

A

R

E

D

E

S

G

G

L

L

V

W

F

F

A

P

V

V

D

D

P
|
P

T
x
G

S
x
A

Q

-

D

D

A

A

S

S

T

-

I

L

G

C

G
|
G

N
x
M

I

A

A
|
A

M
x
T

N

G

A
|
A

G
x
S

G

G

K

T

K

N

A

A

V

V

L

R

W

Y

G

G

T

T

T

M

I

R

D

D

N

N

L

V

A

I

S

N

W

L

R

E

M

V

V

V

T

L

P

P

D

D

A

G

K

R

W

L

L

L

E

-

V

-

E

-

R

-

V

-

G

-

H

H

N

T

L

A

G

G

K

R

I

-

H

-

D

-

Q

-

E

-

T

-

V

-

R

-

F

-

R

-

I

-

T

-

R

-

Y

-

S

-

A

-

D

-

G

-

V

-

T

-

P

-

E

-

G

-

A

-

P

-

E

-

I

-

S

-

A

-

P

-

G

G

Q

R

S

H

F

Y

R

R

K

K

L

S

G

A

L

A

G

G

K

Y

D

N

V

L

T

T

D

G

K

L

H

F

L

V

G

G

G

-

L

-

P

-

G

-

V

-

Q

-

K

S

E
|
E

G
|
G

C

T

D

L

G

G

L

I

I
|
I

T

T

S

S

A

T

V

T

F

L

V

R

L

L

H

H

R

P

M

A

P

P

K

E

H

A

I

T

R

V

T

A

V

A

C

T

L

C

E

A

F

F

Y

-

G

-

D

P

D

S

L

V

H

Q

E

A

A

A

V

V

P

D

A

S

I

T

V

V

E

Q

I

I

K

L

D

Q

R

A

L

-

D

-

G

-

D

-

P

-

Q

A

V

V

R

P

L

V

S

A

G

R

L

I

E

E

H

F

L

L

D

D

E

D

R

V

Y

M

V

M

R

D

A

A

V

C

R

N

Y

R

T

H

P

S

K

K

G

L

A

N

R

C

G

P

E

V

R

A

P

P

K

T

M

L

V

F

L

L

L

-

A

-

D

-

V

-

C

-

S

-

D

E

D

F

E

H

D

G

A

S

V

Q

G

Q

R

T

A

L

A

A

S

E

A

Q

M

L

V

Q

R

R

L

T

A

E

N

A

A

I

R

T

N

Q

G

D

E

N

G

G

F

G

V

S

A

H

V

F

S

S

A

A

E

E

A

K

R

R

K

N

R

E

F

L

W

W

S

A

E

A

R
|
R

-

H

-

N

A

A

R
x
W

T
x
Y

A

A

I

L

A

A

A

L

H

S

T

P

N

G

A

S

F

K

K

A

I

Y

N

S

E

T

D

D

V

V

V

C

I

V

P

P

L

I

D

S

R

R

L

L

A

P

E

E

binding 2-Ketohexanoic acid: V75 (≠ Y208), R317 (= R517), W321 (≠ R519)
binding flavin-adenine dinucleotide: P68 (= P201), G70 (= G203), T71 (≠ G204), G72 (= G205), T73 (= T206), G74 (= G207), G78 (≠ A211), V79 (≠ A212), L90 (≠ T224), P132 (= P274), G133 (≠ T275), A134 (≠ S276), G140 (= G284), M141 (≠ N285), A143 (= A287), T144 (≠ M288), A146 (= A290), S147 (≠ G291), E200 (= E387), G201 (= G388), I206 (= I393), W321 (≠ R519), Y322 (≠ T520)

Sites not aligning to the query:

2uuvC Alkyldihydroxyacetonephosphate synthase in p1 (see paper)
26% identity, 16% coverage: 119:330/1288 of query aligns to 73:265/491 of 2uuvC

query
sites
2uuvC

E

E

A

F

V

V

R

R

R

E

F

L

E

K

D

A

W

F

F

F

-

L

P

P

E

D

Q

Q

R

-

A

-

L

L

R

K

K

D

R

D

T

K

L

L

R

A

R

R

L

I

S

T

K

-

H

H

T

T

R

F

R

G

D

K

N

S

I

L

H

R

F

D

D

L

G

I

F

R

A

V

R

R

V

I

S

G

H

Q

V

V

T

K

D

N

A

A

T

P

D

D

W

-

R

-

V

-

E

-

L

-

P

-

F

-

V

L

V

I

V

V

T

L

P

P

D

H

S

S

E

H

A

E

E

E

M

V

A

E

G

R

V

L

V

V

A

Q

A

L

C

A

I

H

E

K

L

Y

G

N

L

V

T

V

V

I

I

I

P
|
P

R

M

G
|
G

T
x
S

G
x
N

Y
x
I

T

V

G

G

A
|
A

A
x
I

V

E

P

P

L

V

D

E

E

R

R

F

S

T

A

V

V

S

I

I

N

D

T

M

E

R

K

R

L

M

E

N

G

K

L

V

G

L

S

W

V

V

E

D

M

R

R

R

R

E

I

M

P

-

G

-

V

-

E

-

S

-

V

-

P

-

T

T

V

A

R

C

A

I

E

Q

A

V

G

G

V

I

V

M

T

G

R

P

R

E

V

L

S

E

E

K

R

Q

A

L

A

H

E

K

E

Q

G

G

L

V

V

S

F

L

A

G

V

H

D

D

P
|
P

T
x
D

S
|
S

Q

F

D

E

A

F

S
|
S

T
|
T

I

L

G

G

G
|
G

N

W

I

L

A
|
A

M
x
T

N

C

A
x
S

G
x
S

G

G

K

H

K

Q

A

S

V

D

L

K

W

Y

G

G

T

D

T

I

I

E

D

D

N

M

L

A

A

V

S

S

W

F

R

R

M

T

V

V

T

T

P

P

D

T

A

G

K

T

W

-

L

L

E

E

V

L

E

-

R

R

V

A

G

G

H

I

N

N

L

Y

G

K

K

H

I

I

binding flavin-adenine dinucleotide: P146 (= P201), G148 (= G203), G149 (= G204), G150 (= G205), S151 (≠ T206), N152 (≠ G207), I153 (≠ Y208), A156 (= A211), I157 (≠ A212), P211 (= P274), D212 (≠ T275), S213 (= S276), S217 (= S280), T218 (= T281), G221 (= G284), A224 (= A287), T225 (≠ M288), S227 (≠ A290), S228 (≠ G291)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 270, 271, 276, 402

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
24% identity, 30% coverage: 158:545/1288 of query aligns to 25:349/456 of 8jdrA

query
sites
8jdrA

R

R

V

E

S

Q

H

H

V

G

T

H

D

D

A

E

T

S

D

M

W

H

R

R

V

C

E

Q

L

P

P

P

F

D

V

A

V

V

T

W

P

P

D

Q

S

N

E

V

A

D

E

Q

M

V

A

S

G

R

V

V

V

A

A

S

A

L

C

C

I

Y

E

N

L

Q

G

G

L

V

T

P

V

I

I

I

P
|
P

R

F

G
|
G

G
x
T

G
|
G

T
|
T

G
|
G

Y

V

T

E

G

G

A
x
G

A
x
V

V

C

P

A

L

V

D

-

E

Q

R

G

S

G

A

V

V

C

I

I

N

N

T
x
L

E

T

K

H

L

M

E

D

G

Q

L

I

G

T

S

E

V

L

E

N

M

T

R

E

R

D

I

F

P

-

G

-

V

-

E

-

S

-

V

-

P

-

T

S

V

V

R

V

A

V

E

E

A

P

G

G

V

V

V

T

T

R

R

K

R

A

V

L

S

N

E

T

R

H

A

L

A

R

E

D

E

S

G

G

L

L

V

W

F

F

A

P

V

V

D

D

P
|
P

T
x
G

S
x
A

Q

-

D

D

A

A

S

S

T

-

I

L

G

C

G
|
G

N
x
M

I

A

A
|
A

M
x
T

N

G

A
|
A

G
x
S

G

G

K

T

K

N

A

A

V

V

L

R

W

Y

G

G

T

T

T

M

I

R

D

D

N

N

L

V

A

I

S

N

W

L

R

E

M

V

V

V

T

L

P

P

D

D

A

G

K

R

W

L

L

L

E

-

V

-

E

-

R

-

V

-

G

-

H

H

N

T

L

A

G

G

K

R

I

-

H

-

D

-

Q

-

E

-

T

-

V

-

R

-

F

-

R

-

I

-

T

-

R

-

Y

-

S

-

A

-

D

-

G

-

V

-

T

-

P

-

E

-

G

-

A

-

P

-

E

-

I

-

S

-

A

-

P

-

G

G

Q

R

S

H

F

Y

R

R

K

K

L

S

G

A

L

A

G

G

K

Y

D

N

V

L

T

T

D

G

K

L

H

F

L

V

G

G

G

-

L

-

P

-

G

-

V

-

Q

-

K

S

E
|
E

G
|
G

C

T

D

L

G

G

L

I

I
|
I

T

T

S

S

A

T

V

T

F

L

V

R

L

L

H

H

R

P

M

A

P

P

K

E

H

A

I

T

R

V

T

A

V

A

C

T

L

C

E

A

F

F

Y

-

G

-

D

P

D

S

L

V

H

Q

E

A

A

A

V

V

P

D

A

S

I

T

V

V

E

Q

I

I

K

L

D

-

R

-

L

-

D

-

G

-

D

Q

P

A

Q

A

V

V

R

P

L

V

S

A

G

R

L

I

E

E

H

F

L

L

D

D

E

D