SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078210708.1 NCBI__GCF_002017945.1:WP_078210708.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6dbbA Crystal structure of a putative aldehyde dehydrogenase family protein burkholderia cenocepacia j2315 in complex with partially reduced nadh
56% identity, 93% coverage: 36:517/517 of query aligns to 20:504/504 of 6dbbA

query
sites
6dbbA

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active site: N152 (= N168), E259 (= E269), C293 (= C303), E471 (= E484)
binding nicotinamide-adenine-dinucleotide: I148 (= I164), S149 (= S165), A150 (= A166), F151 (= F167), N152 (= N168), K175 (= K191), S177 (= S193), R218 (≠ Y228), T236 (= T246), G237 (= G247), S238 (= S248), M241 (= M251), E259 (= E269), L260 (= L270), G261 (= G271), C293 (= C303), E391 (= E403), F393 (= F405)
binding beta-6-hydroxy-1,4,5,6-tetrhydronicotinamide adenine dinucleotide: I148 (= I164), S149 (= S165), A150 (= A166), F151 (= F167), N152 (= N168), K175 (= K191), S177 (= S193), R218 (≠ Y228), T236 (= T246), G237 (= G247), S238 (= S248), M241 (= M251), E259 (= E269), L260 (= L270), G261 (= G271), C293 (= C303), E391 (= E403), F393 (= F405)

2jg7A Crystal structure of seabream antiquitin and elucidation of its substrate specificity (see paper)
53% identity, 97% coverage: 14:515/517 of query aligns to 13:509/509 of 2jg7A

query
sites
2jg7A

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active site: N166 (= N168), K189 (= K191), E267 (= E269), C301 (= C303), E398 (= E403), E478 (= E484)
binding nicotinamide-adenine-dinucleotide: I162 (= I164), T163 (≠ S165), A164 (= A166), F165 (= F167), N166 (= N168), K189 (= K191), P192 (≠ E194), A226 (≠ Y228), G229 (= G231), T230 (≠ E232), F243 (≠ A245), T244 (= T246), G245 (= G247), S246 (= S248), V249 (≠ M251), E267 (= E269), L268 (= L270), C301 (= C303), E398 (= E403), F400 (= F405)

P49419 Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 from Homo sapiens (Human) (see 5 papers)
52% identity, 100% coverage: 1:515/517 of query aligns to 29:538/539 of P49419

query
sites
P49419

M

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A
|
A

F
|
F

N
|
N

F
|
F

P
|
P

V
|
V

A
|
A

V
|
V

W
x
Y

A
x
G

W
|
W

N
|
N

T
x
N

A
|
A

L
x
I

A
|
A

W
x
M

I
|
I

C
|
C

G
|
G

D
x
N

V
|
V

C
|
C

V
x
L

W
|
W

K
|
K

P
x
G

S
x
A

E
x
P

K
x
T

T
|
T

P
x
S

L
|
L

C
x
I

G
x
S

V
|
V

A
|
A

C
x
V

Q
x
T

N
x
K

I
|
I

I
|
I

A
|
A

E
x
K

V
|
V

L
|
L

K
x
E

E
x
D

N
|
N

N
x
K

L
|
L

P
|
P

E
x
G

G
x
A

I
|
I

S
x
C

C
x
S

L
|
L

I
x
T

N
x
C

G
|
G

D
x
G

Y
x
A

T
x
D

I
|
I

G
|
G

E
x
T

M
x
A

L
x
M

T
x
A

K
|
K

D
|
D

T
x
E

R
|
R

V
|
V

P
x
N

L
|
L

I
x
L

S
|
S

A
x
F

T
|
T

G
|
G

S
|
S

T
|
T

R
x
Q

M
x
V

G
|
G

K
|
K

I
x
Q

V
|
V

A
x
G

Q
x
L

T
x
M

V
|
V

A
x
Q

G
x
E

R
|
R

L
x
F

G
|
G

K
x
R

S
|
S

L
|
L

L
|
L

E
|
E

L
|
L

G
|
G

G
|
G

N
|
N

N
|
N

A
|
A

I
|
I

I
|
I

V
x
A

T
x
F

P
x
E

D
|
D

A
|
A

D
|
D

I
x
L

K
x
S

M
x
L

T
x
V

V
|
V

I
x
P

G
x
S

A
|
A

V
x
L

F
|
F

G
x
A

A
|
A

V
|
V

G
|
G

T
|
T

A
|
A

G
|
G

Q
|
Q

R
|
R

C
|
C

T
|
T

S
x
T

T
x
A

R
|
R

R
|
R

L
|
L

I
x
F

I
|
I

H
|
H

E
|
E

S
|
S

M
x
I

Y
x
H

D
|
D

K
x
E

V
|
V

K
x
V

D
x
N

T
x
R

I
x
L

V
x
K

A
x
K

A
|
A

Y
|
Y

G
x
A

Q
|
Q

L
x
I

R
|
R

I
x
V

G
|
G

N
|
N

P
|
P

L
x
W

D
|
D

E
x
P

K
x
N

N
x
V

H
x
L

V
x
Y

G
|
G

P
|
P

L
|
L

I
x
H

D
x
T

K
|
K

Q
|
Q

A
|
A

V
|
V

E
x
S

M
|
M

Y
x
F

N
x
L

Q
x
G

A
|
A

L
x
V

T
x
E

K
x
E

V
x
A

V
x
K

A
x
K

E
|
E

G
|
G

G
|
G

K
x
T

I
x
V

L
x
V

V
x
Y

E
x
G

G
|
G

G
x
K

V
|
V

L
x
M

S
x
D

G

-

E

-

G

-

Y

-

E
x
R

S
x
P

G
|
G

C
x
N

Y
|
Y

V
|
V

K
x
E

P
|
P

A
x
T

I
|
I

A
x
V

E
x
T

A
x
G

Q
x
L

N
x
G

S
x
H

F
x
D

E
x
A

I
x
S

V
x
I

Q
x
A

H
|
H

-
x
T

E
|
E

T
|
T

F
|
F

A
|
A

P
|
P

V
x
I

L
|
L

Y
|
Y

L
x
V

L
x
F

K
|
K

Y
x
F

S
x
K

G
x
N

D
x
E

V

-

D
x
E

N
x
E

A
x
V

I
x
F

K
x
A

L
x
W

Q
x
N

N
|
N

G
x
E

V
|
V

A
x
K

Q
|
Q

G
|
G

L
|
L

S
|
S

S
|
S

A
x
S

I
|
I

M
x
F

T
|
T

N
x
K

N
x
D

L
|
L

R
x
G

E
x
R

A
x
I

E
x
F

R
|
R

F
x
W

L
|
L

S
x
G

V
x
P

A
x
K

G
|
G

S
|
S

D
|
D

C
|
C

G
|
G

I
|
I

A
x
V

N
|
N

V
|
V

N
|
N

I
|
I

G
x
P

T
|
T

S
|
S

G
|
G

A
|
A

E
|
E

I
|
I

G
|
G

G
|
G

A
|
A

F
|
F

G
|
G

G
|
G

E
|
E

K
|
K

E
x
H

T
|
T

G
|
G

G
|
G

G
|
G

R
|
R

E
|
E

S
|
S

G
|
G

S
|
S

D
|
D

A
|
A

W
|
W

K
|
K

V
x
Q

Y
|
Y

M
|
M

R
|
R

R
|
R

Q
x
S

T
|
T

N
x
C

T
|
T

I
|
I

N
|
N

Y
|
Y

T
x
S

T
x
K

N
x
D

L
|
L

P
|
P

L
|
L

A
|
A

Q
|
Q

G
|
G

I
|
I

K
|
K

F
|
F

TAF 192:194 (≠ SAF 165:167) binding NAD(+)
A199 (= A172) to V: in EPEO4; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912709
K218 (= K191) binding NAD(+)
GT 258:259 (≠ GE 231:232) binding NAD(+)
GS 274:275 (= GS 247:248) binding NAD(+)
EL 296:297 (= EL 269:270) binding NAD(+)
C330 (= C303) active site, Nucleophile
E427 (= E403) binding NAD(+); to Q: in EPEO4; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912707
K439 (≠ S415) to Q: in dbSNP:rs12514417

Sites not aligning to the query:

110:539 natural variant: Missing (in EPEO4; loss of alpha-AASA dehydrogenase activity)

4zulA Structure aldh7a1 complexed with alpha-aminoadipate (see paper)
53% identity, 97% coverage: 14:515/517 of query aligns to 12:508/509 of 4zulA

query
sites
4zulA

L

L

A

K

Q

E

L

L

G

G

I

L

K

R

A

E

V

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

T

S

Q

W

H

G

F

-

A

G

S

R

G

G

S

E

L

V

L

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

Q

E

L

P

I

I

A

A

S

R

V

V

T

R

T

Q

S

A

S

S

A

V

A

A

D

D

Y

Y

E

E

K

E

V

T

M

V

Q

K

T

K

A

A

T

R

A

E

A

A

F

W

K

K

T

I

F

W

R

A

L

D

M

I

P

P

A

A

P

P

Q

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

E

D

K

A

L

L

R

R

K

E

N

K

K

I

E

Q

A

V

L

L

G

G

K

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

Y

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

H

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

G

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

S

P

L

V

G

G

V

L

V

V

G

G

I

I

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

Y

A

G

W

W

N

N

T

N

A

A

L

I

A

A

W

M

I

I

C

C

G

G

D

N

V

V

C

C

V

L

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

V

V

A

A

C

V

Q

T

N

K

I

I

I

I

A

A

E

K

V

V

L

L

K

E

E

D

N

N

N

K

L

L

P

P

E

G

G

A

I

I

S

C

C

S

L

L

I

T

N

C

G

G

D

G

Y

A

T

D

I

I

G

G

E

T

M

A

L

M

T

A

K

K

D

D

T

E

R

R

V

V

P

N

L

L

I

L

S

S

A

F

T

T

G

G

S

S

T

T

R

Q

M

V

G

G

K

K

I

Q

V

V

A

G

Q

L

T

M

V

V

A

Q

G

E

R

R

L

F

G

G

K

R

S

S

L

L

L

L

E
|
E

L

L

G

G

G

G

N

N

N

N

A

A

I

I

I

I

V

A

T

F

P

E

D

D

A

A

D

D

I

L

K

S

M

L

T

V

V

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

S

T

T

A

R

R

R

R

L

L

I

F

I

I

H

H

E

E

S

S

M

I

Y

H

D

D

K

E

V

V

K

V

D

N

T

R

I

L

V

K

A

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

N

N

P

P

L

W

D

D

E

P

K

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

Q

Q

A

A

V

V

E

S

M

M

Y

F

N

L

Q

G

A

A

L

V

T

E

K

E

V

A

V

K

A

K

E

E

G

G

G

G

K

T

I

V

L

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

A

T

I

I

A

V

E

T

A

G

Q

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F

F

A

A

P

P

V

I

L

L

Y

Y

L

V

L

F

K

K

Y

F

S

K

G

N

D

E

V

-

D

E

N

E

A

V

I

F

K

A

L

W

Q

N

N

N

G

E

V

V

A

K

Q

Q

G

G

L

L

S

S

S

S

A

S

I

I

M

F

T

T

N

K

N

D

L

L

R

G

E

R

A

I

E

F

R

R

F

W

L

L

S

G

V

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G
|
G

A
|
A

E

E

I

I

G

G

G

G

A

A

F
|
F

G

G

G

G

E

E

K

K

E

H

T

T

G

G

G

G

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

V

Q

Y

Y

M

M

R

R

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

T

S

T

K

N

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

active site: N165 (= N168), K188 (= K191), E266 (= E269), C300 (= C303), E397 (= E403), E477 (= E484)
binding 2-aminohexanedioic acid: E119 (= E122), F166 (= F169), R299 (= R302), C300 (= C303), T301 (= T304), G459 (= G466), A460 (= A467), F466 (= F473)

4x0tA Structure aldh7a1 inactivated by 4-diethylaminobenzaldehyde and complexed with NAD+ (see paper)
53% identity, 97% coverage: 14:515/517 of query aligns to 12:508/509 of 4x0tA

query
sites
4x0tA

L

L

A

K

Q

E

L

L

G

G

I

L

K

R

A

E

V

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

T

S

Q

W

H

G

F

-

A

G

S

R

G

G

S

E

L

V

L

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

Q

E

L

P

I

I

A

A

S

R

V

V

T

R

T

Q

S

A

S

S

A

V

A

A

D

D

Y

Y

E

E

K

E

V

T

M

V

Q

K

T

K

A

A

T

R

A

E

A

A

F

W

K

K

T

I

F

W

R

A

L

D

M

I

P

P

A

A

P

P

Q

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

E

D

K

A

L

L

R

R

K

E

N

K

K

I

E

Q

A

V

L

L

G

G

K

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

Y

V

G

G

E

E

V

V

Q

Q

E

E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

H

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

G

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

S

P

L

V

G

G

V

L

V

V

G

G

I

I

I

I

S
x
T

A
|
A

F
|
F

N
|
N

F
|
F

P

P

V

V

A

A

V
|
V

W

Y

A

G

W
|
W

N

N

T

N

A

A

L

I

A

A

W

M

I

I

C

C

G

G

D

N

V

V

C

C

V

L

W

W

K
|
K

P
x
G

S
x
A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

V

V

A

A

C

V

Q

T

N

K

I

I

I

I

A

A

E

K

V

V

L

L

K

E

E

D

N

N

N

K

L

L

P

P

E

G

G

A

I

I

S

C

C

S

L

L

I

T

N

C

G

G

D

G

Y
x
A

T

D

I

I

G
|
G

E
x
T

M

A

L

M

T

A

K

K

D

D

T

E

R

R

V

V

P

N

L

L

I

L

S

S

A
x
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

M
x
V

G

G

K

K

I

Q

V

V

A

G

Q

L

T

M

V

V

A

Q

G

E

R

R

L

F

G

G

K

R

S

S

L

L

L

L

E
|
E

L
|
L

G

G

G

G

N

N

N

N

A

A

I

I

I

I

V

A

T

F

P

E

D

D

A

A

D

D

I

L

K

S

M

L

T

V

V

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

S

T

T

A

R

R

R

R

L

L

I

F

I

I

H

H

E

E

S

S

M

I

Y

H

D

D

K

E

V

V

K

V

D

N

T

R

I

L

V

K

A

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

N

N

P

P

L

W

D

D

E

P

K

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

Q

Q

A

A

V

V

E

S

M

M

Y

F

N

L

Q

G

A

A

L

V

T

E

K

E

V

A

V

K

A

K

E

E

G

G

G

G

K

T

I

V

L

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

A

T

I

I

A

V

E

T

A

G

Q

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F
|
F

A

A

P

P

V

I

L

L

Y

Y

L

V

L

F

K

K

Y

F

S

K

G

N

D

E

V

-

D

E

N

E

A

V

I

F

K

A

L

W

Q

N

N

N

G

E

V

V

A

K

Q

Q

G

G

L

L

S

S

S

S

A

S

I

I

M

F

T

T

N

K

N

D

L

L

R

G

E

R

A

I

E

F

R

R

F

W

L

L

S

G

V

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G

G

A

A

E

E

I

I

G

G

G

G

A

A

F
|
F

G

G

G

G

E

E

K

K

E

H

T

T

G

G

G

G

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

V

Q

Y

Y

M

M

R

R

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

T

S

T

K

N

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

active site: N165 (= N168), K188 (= K191), E266 (= E269), C300 (= C303), E397 (= E403), E477 (= E484)
binding 4-(diethylamino)benzaldehyde: F166 (= F169), V170 (= V173), W173 (= W176), C300 (= C303), F466 (= F473)
binding nicotinamide-adenine-dinucleotide: T162 (≠ S165), A163 (= A166), F164 (= F167), N165 (= N168), K188 (= K191), G189 (≠ P192), A190 (≠ S193), A225 (≠ Y228), G228 (= G231), T229 (≠ E232), F242 (≠ A245), T243 (= T246), G244 (= G247), S245 (= S248), V248 (≠ M251), E266 (= E269), L267 (= L270), C300 (= C303), E397 (= E403), F399 (= F405)

6o4dB Structure of aldh7a1 mutant w175a complexed with l-pipecolic acid (see paper)
53% identity, 97% coverage: 14:515/517 of query aligns to 13:509/510 of 6o4dB

query
sites
6o4dB

L

L

A

K

Q

E

L

L

G

G

I

L

K

R

A

E

V

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

T

S

Q

W

H

G

F

-

A

G

S

R

G

G

S

E

L

V

L

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

Q

E

L

P

I

I

A

A

S

R

V

V

T

R

T

Q

S

A

S

S

A

V

A

A

D

D

Y

Y

E

E

K

E

V

T

M

V

Q

K

T

K

A

A

T

R

A

E

A

A

F

W

K

K

T

I

F

W

R

A

L

D

M

I

P

P

A

A

P

P

Q

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

E

D

K

A

L

L

R

R

K

E

N

K

K

I

E

Q

A

V

L

L

G

G

K

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

Y

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

H

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

G

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

S

P

L

V

G

G

V

L

V

V

G

G

I

I

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

Y

A

G

W

A

N

N

T

N

A

A

L

I

A

A

W

M

I

I

C

C

G

G

D

N

V

V

C

C

V

L

W

W

K

K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

V

V

A

A

C

V

Q

T

N

K

I

I

I

I

A

A

E

K

V

V

L

L

K

E

E

D

N

N

N

K

L

L

P

P

E

G

G

A

I

I

S

C

C

S

L

L

I

T

N

C

G

G

D

G

Y

A

T

D

I

I

G

G

E

T

M

A

L

M

T

A

K

K

D

D

T

E

R

R

V

V

P

N

L

L

I

L

S

S

A

F

T

T

G

G

S

S

T

T

R

Q

M

V

G

G

K

K

I

Q

V

V

A

G

Q

L

T

M

V

V

A

Q

G

E

R

R

L

F

G

G

K

R

S

S

L

L

L

L

E
|
E

L

L

G

G

G

G

N

N

N

N

A

A

I

I

I

I

V

A

T

F

P

E

D

D

A

A

D

D

I

L

K

S

M

L

T

V

V

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T
|
T

S

T

T

A

R

R

R

R

L

L

I

F

I

I

H

H

E

E

S

S

M

I

Y

H

D

D

K

E

V

V

K

V

D

N

T

R

I

L

V

K

A

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

N

N

P

P

L

W

D

D

E

P

K

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

Q

Q

A

A

V

V

E

S

M

M

Y

F

N

L

Q

G

A

A

L

V

T

E

K

E

V

A

V

K

A

K

E

E

G

G

G

G

K

T

I

V

L

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

A

T

I

I

A

V

E

T

A

G

Q

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E

E

T

T

F

F

A

A

P

P

V

I

L

L

Y

Y

L

V

L

F

K

K

Y

F

S

K

G

N

D

E

V

-

D

E

N

E

A

V

I

F

K

A

L

W

Q

N

N

N

G

E

V

V

A

K

Q

Q

G

G

L

L

S

S

S

S

A

S

I

I

M

F

T

T

N

K

N

D

L

L

R

G

E

R

A

I

E

F

R

R

F

W

L

L

S

G

V

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G
|
G

A
|
A

E

E

I

I

G

G

G

G

A

A

F
|
F

G

G

G

G

E

E

K

K

E

H

T

T

G

G

G

G

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

V

Q

Y

Y

M

M

R

R

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

T

S

T

K

N

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

active site: N166 (= N168), E267 (= E269), C301 (= C303), E478 (= E484)
binding pyridine-2-carboxylic acid: E120 (= E122), F167 (= F169), T302 (= T304), G460 (= G466), A461 (= A467), F467 (= F473)

4pxnA Structure of zm aldh7 in complex with NAD (see paper)
51% identity, 97% coverage: 11:510/517 of query aligns to 4:498/498 of 4pxnA

query
sites
4pxnA

N

H

E

Q

A

F

L

L

A

A

Q

E

L

L

G

G

I

L

K

A

A

Q

V

R

N

N

E

P

G

G

T

A

S

F

T

A

G

C

T

G

Q

A

H

W

F

G

A

G

S

S

G

G

S

P

L

T

L

V

E

T

S

S

Y

T

S

S

P

P

V

T

D

N

G

N

Q

Q

L

V

I

I

A

A

S

E

V

V

T

V

T

E

S

A

S

S

A

V

A

H

D

D

Y

Y

E

E

K

E

V

G

M

M

Q

R

T

A

A

C

T

F

A

D

A

A

F

A

K

K

T

T

F

W

R

M

L

A

M

I

P

P

A

A

P

P

Q

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

E

D

K

A

L

L

R

R

K

A

N

K

K

L

E

H

A

H

L

L

G

G

K

R

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

P

E

E

G

G

Y

I

G

G

E

E

V

V

Q

Q

E

E

M

I

I

I

D

D

I

M

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

H

N

G

G

L

S

T

I

M

I

H

P

S

S

E

E

R

R

P

P

G

N

H

H

R

M

M

M

Y

M

E

E

Q

V

Y

W

H

N

S

P

L

L

G

G

V

V

V

V

G

G

I

V

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F

F

P

P

V

C

A

A

V

V

W

L

A

G

W

W

N

N

T

A

A

C

L

I

A

A

W

L

I

V

C

C

G

G

D

N

V

C

C

V

V

V

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

P

L

L

C

I

G

T

V

I

A

A

C

M

Q

T

N

K

I

I

I

V

A

A

E

S

V

V

L

L

K

E

E

K

N

N

N

N

L

L

P

P

E

G

G

A

I

I

S

F

C

T

L

S

I

F

N

C

G

G

D

G

Y
x
T

T

E

I

I

G
|
G

E
x
Q

M

A

L

I

T

A

K

L

D

D

T

I

R

R

V

I

P

P

L

L

I

V

S

S

A
x
F

T
|
T

G
|
G

S
|
S

T

T

R

R

M
x
A

G

G

K

L

I

M

V
|
V

A

Q

Q

Q

T

Q

V

V

A

S

G

A

R

R

L

F

G

G

K

K

S

C

L

L

L

L

E
|
E

L
|
L

G
x
S

G

G

N

N

N

N

A

A

I

I

I

I

V

V

T

M

P

D

D

D

A

A

D

D

I

I

K

Q

M

L

T

A

V

V

I

R

G

S

A

V

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

S

T

T

C

R

R

R

R

L

L

I

I

I

L

H

H

E

E

S

N

M

I

Y

Y

D

Q

K

T

V

F

K

L

D

D

T

Q

I

L

V

V

A

E

A

V

Y

Y

G

K

Q

Q

L

V

R

R

I

I

G

G

N

D

P

P

L

L

D

E

E

K

K

G

N

T

H

L

V

L

G

G

P

P

L

L

I

H

D

T

K

P

Q

A

A

S

V

K

E

E

M

N

Y

F

N

L

Q

K

A

G

L

I

T

Q

K

T

V

I

V

K

A

S

E

Q

G

G

G

G

K

K

I

I

L

L

V

F

E

G

G

G

G

S

V

A

L

I

S

E

G

S

E

E

G

G

Y

N

E

-

S

-

G

-

C

-

Y

F

V

V

K

Q

P

P

A

T

I

I

A

V

E

E

A

I

Q

T

N

P

S

S

F

A

E

P

I

V

V

V

Q

K

H

E

E
|
E

T

L

F
|
F

A

G

P

P

V

V

L

L

Y

Y

L

V

L

M

K

K

Y

F

S

Q

G

-

D

T

V

L

D

K

N

E

A

A

I

I

K

E

L

I

Q

N

N

N

G

S

V

V

A

P

Q

Q

G

G

L

L

S

S

S

S

A

S

I

I

M

F

T

T

N

K

N

R

L

P

R

D

E

I

A

I

E

F

R

K

F

W

L

L

S

G

V

P

A

H

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

N

G

G

A

A

E

E

I

I

G

G

G

G

A

A

F
|
F

G

G

G

G

E

E

K

K

E

A

T

T

G

G

G

G

G

G

R

R

E
|
E

S

A

G

G

S

S

D

D

A

S

W

W

K

K

V

Q

Y

Y

M

M

R

R

R

R

Q

A

T

T

N

C

T

T

I

I

N

N

Y

Y

T

G

T

S

N

E

L

L

P

P

L

L

A

A

active site: N161 (= N168), K184 (= K191), E262 (= E269), C296 (= C303), E392 (= E403), E472 (= E484)
binding nicotinamide-adenine-dinucleotide: I157 (= I164), T158 (≠ S165), A159 (= A166), F160 (= F167), N161 (= N168), K184 (= K191), T221 (≠ Y228), G224 (= G231), Q225 (≠ E232), F238 (≠ A245), T239 (= T246), G240 (= G247), S241 (= S248), A244 (≠ M251), V248 (= V255), E262 (= E269), L263 (= L270), S264 (≠ G271), C296 (= C303), E392 (= E403), F394 (= F405), F461 (= F473)

2j6lA Structure of aminoadipate-semialdehyde dehydrogenase (see paper)
52% identity, 95% coverage: 14:504/517 of query aligns to 12:497/497 of 2j6lA

query
sites
2j6lA

L

L

A

K

Q

E

L

L

G

G

I

L

K

R

A

E

V

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

T

S

Q

W

H

G

F

-

A

G

S

R

G

G

S

E

L

V

L

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

Q

E

L

P

I

I

A

A

S

R

V

V

T

R

T

Q

S

A

S

S

A

V

A

A

D

D

Y

Y

E

E

K

E

V

T

M

V

Q

K

T

K

A

A

T

R

A

E

A

A

F

W

K

K

T

I

F

W

R

A

L

D

M

I

P

P

A

A

P

P

Q

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

E

D

K

A

L

L

R

R

K

E

N

K

K

I

E

Q

A

V

L

L

G

G

K

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

Y

V

G

G

E

E

V

V

Q

Q

E

E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

H

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

G

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

S

P

L

V

G

G

V

L

V

V

G

G

I

I

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F

F

P

P

V

V

A

A

V

V

W

Y

A

G

W

W

N

N

T

N

A

A

L

I

A

A

W

M

I

I

C

C

G

G

D

N

V

V

C

C

V

L

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

V

V

A

A

C

V

Q

T

N

K

I

I

I

I

A

A

E

K

V

V

L

L

K

E

E

D

N

N

N

K

L

L

P

P

E

G

G

A

I

I

S

C

C

S

L

L

I

T

N

C

G

G

D

G

Y
x
A

T

D

I

I

G
|
G

E
x
T

M

A

L

M

T

A

K

K

D

D

T

E

R

R

V

V

P

N

L

L

I

L

S

S

A
x
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

M
x
V

G

G

K

K

I

Q

V

V

A

G

Q

L

T

M

V

V

A

Q

G

E

R

R

L

F

G

G

K

R

S

S

L

L

L

L

E
|
E

L
|
L

G

G

G

G

N

N

N

N

A

A

I

I

I

I

V

A

T

F

P

E

D

D

A

A

D

D

I

L

K

S

M

L

T

V

V

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

S

T

T

A

R

R

R

R

L

L

I