SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078211275.1 NCBI__GCF_002017945.1:WP_078211275.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 82% coverage: 29:243/261 of query aligns to 30:245/265 of P07821

query
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P07821

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K50 (= K49) mutation to Q: Lack of activity.
D172 (= D171) mutation to E: Lack of activity.
E173 (= E172) mutation to A: Lack of activity.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 83% coverage: 4:220/261 of query aligns to 2:213/280 of 5x40A

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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y16), V18 (= V21), A20 (≠ T23), N40 (= N45), G41 (= G46), G43 (= G48), K44 (= K49), S45 (= S50), T46 (= T51), Q88 (≠ T91), H139 (≠ Y145), M140 (≠ Q146), L141 (≠ I147), S142 (= S148), G144 (= G150), Q145 (= Q151), Q166 (≠ E172), H198 (= H205)
binding magnesium ion: S45 (= S50), Q88 (≠ T91)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
30% identity, 82% coverage: 5:217/261 of query aligns to 1:213/222 of 7arlD

query
sites
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binding adenosine-5'-diphosphate: G42 (= G46), G44 (= G48), K45 (= K49), S46 (= S50)

7mdyC Lolcde nucleotide-bound
30% identity, 82% coverage: 5:217/261 of query aligns to 1:213/226 of 7mdyC

query
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7mdyC

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binding adp orthovanadate: Y12 (= Y16), G42 (= G46), S43 (≠ I47), G44 (= G48), K45 (= K49), S46 (= S50), T47 (= T51), Q91 (≠ T91), H138 (≠ K142), E142 (≠ Q146), S144 (= S148), G145 (≠ D149), G146 (= G150), E168 (= E172), N172 (≠ H176), H201 (= H205)
binding magnesium ion: S46 (= S50), Q91 (≠ T91)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
30% identity, 82% coverage: 5:217/261 of query aligns to 4:216/233 of P75957

query
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P75957

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G42 (= G43) mutation to D: Loss of lipoprotein release when overexpressed.

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
30% identity, 82% coverage: 5:217/261 of query aligns to 3:215/229 of 7v8iD

query
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P

P

Y

L

T

L

N

I

W

G

I

K

G

K

T

K

L

P

T

A

E

E

E

I

D

N

K

S

I

R

Q

A

V

L

E

E

R

-

A

M

L

L

S

K

L

A

T

V

Q

G

I

L

S

D

H

H

L
x
R

A

A

D

N

K
x
H

K

R

H

P

Y

S

Q
x
E

I

L

S
|
S

D

G

G
|
G

Q
x
E

L

R

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

D

N

T

P

P

R

L

L

I

V

I

L

L

A

D

D

E

Q

P

P

T

T

T

G

H

N

L

L

D

D

L

A

L

R

H

N

K

A

V

D

S

S

L

I

L

F

K

Q

L

L

K

G

K

E

L

L

T

N

H

R

E

L

T

Q

G

G

K

T

C

A

I

F

L

L

F

V

S

V

T

T

H
|
H

D

D

I

L

D

Q

M

L

A

A

I

K

Q

R

L

M

S

S

D

R

E

Q

M

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V25), S43 (≠ N45), G44 (= G46), G46 (= G48), K47 (= K49), S48 (= S50), T49 (= T51), Q93 (≠ T91), R137 (≠ L139), H140 (≠ K142), E144 (≠ Q146), S146 (= S148), G148 (= G150), E149 (≠ Q151), H203 (= H205)
binding magnesium ion: S48 (= S50), Q93 (≠ T91)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 82% coverage: 25:237/261 of query aligns to 32:246/378 of P69874

query
sites
P69874

V

V

A

I

K

P

E

Q

V

L

S

D

I

L

A

T

L

I

K

N

G

G

K

E

L
x
F

T

L

A

T

L

L

I

L

G

G

A

P

N

S

G

G

I
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

K

R

T

L

I

I

T

A

G

G

I

L

Q

E

T

T

P

V

I

D

E

S

G

G

D

R

V

I

L

M

L
|
L

H

D

G

N

K

E

N

D

I

I

A

T

I

H

Y

V

S

P

A

A

S

E

D

N

L

-

S

-

Q

R

E

Y

M

V

S

N

L

T

V

V

L

F

T

Q

E

S

K

Y

L

A

P

L

P

F

S

P

N

H

L

M

T

T

V

V

Y

F

E

E

L

N

V

V

A

A

L

F

G

G

R

L

Q

R

P

M

Y

Q

T

K

N

T

W

P

I

A

G

A

T

E

L

I

T

T

E

P

E

-

D

-

K

-

I

-

Q

R

V

V

E

M

R

E

A

A

L

L

S

R

L

M

T
x
V

Q

Q

I

L

S

E

H

T

L

F

A

A

D

Q

K

R

K

K

H

P

Y

H

Q

Q

I

L

S

S

D

G

G

G

Q

Q

L

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

V

A

V

Q

N

D

K

T

P

P

R

L

L

I

L

L

L

D
|
D

E

E

P

S

T

L

T

S

H

A

L

L

D

D

L

Y

L

K

H

L

K

R

V

K

S

Q

L

M

L

Q

K

N

L

E

L

L

K

K

K

A

L

L

T

Q

H

R

E

K

T

L

G

G

K

I

C

T

I

F

L

V

F

F

S

V

T

T

H

H

D

D

I

Q

D

E

M

E

A

A

I

L

Q

T

L

M

S

S

D

D

E

R

M

I

I

V

M

M

T

R

P

D

E

G

T

R

V

I

V

E

Q

Q

D

D

-

G

-

T

-

P

-

R

-

E

-

I

-

Y

-

E

E

E

P

P

C

K

N

N

L

L

I

F

A

V

K

A

G

G

F45 (≠ L38) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ I47) mutation to T: Loss of ATPase activity and transport.
L60 (= L53) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L69) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ T134) mutation to M: Loss of ATPase activity and transport.
D172 (= D171) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
33% identity, 93% coverage: 9:252/261 of query aligns to 1:246/350 of 3fvqB

query
sites
3fvqB

T

T

E

A

S

A

I

L

S

H

I

I

G

G

Y

H

Q

L

N

S

K

K

K

S

V
x
F

-
x
Q

S

N

T
|
T

L

P

V
|
V

A

L

K

N

E

D

V

I

S

S

I

L

A

S

L

L

K

D

K

P

G

G

K

E

L

I

T

L

A

F

L

I

I

I

G

G

A

A

N
x
S

G
|
G

I
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

K

R

T

C

I

L

T

A

G

G

I

F

Q

E

T

Q

P

P

I

D

E

S

G

G

D

E

V

I

L

S

L

L

H

S

G

G

K

K

N

T

I

I

A

F

I

S

Y

K

S

N

A

T

S

N

D

L

L

P

S

V

Q

R

E

E

M

R

S

R

L

L

-

G

-

Y

V

L

L

V

T
x
Q

E

E

K

G

L

V

P

L

P

F

S

P

N

H

L

L

T

T

V

V

Y

Y

E

R

L

N

V

I

A

A

L

Y

G

G

R

-

Q

-

P

-

Y

L

T

G

N

N

W

G

I

K

G

G

T

R

L

-

T

T

E

A

E

Q

D

E

K

R

I

Q

Q

R

V

I

E

E

R

A

A

M

L

L

S

E

L

L

T

T

Q

G

I

I

S

S

H

E

L

L

A

A

D

G

K
x
R

K

Y

H

P

Y

H

Q
x
E

I

L

S
|
S

D

G

G
|
G

Q
|
Q

L

Q

Q

Q

K

R

V

A

L

A

I

L

A

A

R

R

A

A

L

L

A

A

Q

P

D

D

T

P

P

E

L

L

I

I

I

L

L

L

D

D

E

E

P

P

T

F

T

S

H

A

L

L

D

D

L

E

L

Q

H

L

K

R

V

R

S

Q

L

I

L

R

K

E

L

D

L

M

K

I

K

A

L

A

T

L

H

R

E

A

T

N

G

G

K

K

C

S

I

A

L

V

F

F

S

V

T

S

H

H

D

D

I

R

D

E

M

E

A

A

I

L

Q

Q

L

Y

S

A

D

D

E

R

M

I

I

A

I

V

M

M

T

K

P

Q

E

G

T

R

V

I

V

L

Q

Q

-

T

-

A

-

S

-

P

-

H

-

E

-

L

-

Y

D

R

E

Q

P

P

C

A

N

D

L

L

I

D

A

A

K

-

G

-

S

-

F

-

N

-

T

A

L

L

F

F

K

I

D

G

E

E

H

G

I

I

V

V

F

F

D

P

A

A

binding adenosine-5'-triphosphate: F13 (≠ V21), Q14 (vs. gap), T16 (= T23), V18 (= V25), S38 (≠ N45), G39 (= G46), C40 (≠ I47), G41 (= G48), K42 (= K49), T43 (≠ S50), T44 (= T51), R133 (≠ K142), E137 (≠ Q146), S139 (= S148), G141 (= G150), Q142 (= Q151)
binding calcium ion: T43 (≠ S50), Q86 (≠ T91)

8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
33% identity, 83% coverage: 6:221/261 of query aligns to 4:212/257 of 8wm7D

query
sites
8wm7D

I

F

L

L

Q

V

T

V

E

E

S

G

I

V

S

S

I

K

G

I

Y
|
Y

Q

P

N

T

K

P

K

E

V

G

S

P

T
x
Y

L

T

V
|
V

A

L

K

D

E

G

V

I

S

D

I

L

A

K

L

V

K

R

K

E

G

G

K

E

L

F

T

V

A

C

L

L

I

I

G

G

A

H

N
x
S

G
|
G

I
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

N

T

M

I

I

T

S

G

G

I

F

Q

N

T

T

P

P

I

S

E

E

G

G

D

V

V

V

L

L

H

Q

G

D

K

K

N

P

I

I

A

-

I

-

Y

-

S

-

A

-

S

T

D

E

L

P

S

G

Q

P

E

D

M

R

S

M

L

M

V

V

L

F

T

Q

E

N

K

Y

L

C

P

L

S

P

N

W

L

L

T

N

V

V

Y

F

E

E

L

N

V

V

A

Y

L

L

G

A

R

V

Q

D

P

-

Y

-

T

A

N

V

W

F

I

P

G

N

T

K

L

P

T

Q

E

A

E

E

D

K

K

R

I

A

Q

I

V

V

E

R

R

E

A

H

L

L

S

A

L

M

T

V

Q

G

I

L

S

T

H

E

L

A

A

A

D

E

K

K

H

P

Y

S

Q

Q

I

I

S

S

D

G

G

G

Q

M

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

S

Q

I

D

R

T

P

P

Q

L

V

I

L

I

I

L

L

D

D

E

E

P

P

T

F

T

G

H

A

L

L

D

D

L

A

L

I

H

T

K

K

V

E

S

E

L

L

L

Q

K

E

L

E

L

L

K

L

K

Q

L

I

T

W

H

S

E

D

T

H

G

Q

K

V

C

T

I

V

L

L

F

M

S

I

T

T

H

H

D

D

I

I

D

D

M

E

A

A

I

L

Q

F

L

L

S

A

D

D

E

R

M

V

I

V

I

M

M

M

T

T

binding adenosine-5'-diphosphate: Y14 (= Y16), Y21 (≠ T23), V23 (= V25), S43 (≠ N45), G44 (= G46), C45 (≠ I47), G46 (= G48), K47 (= K49), S48 (= S50), T49 (= T51)

8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
32% identity, 83% coverage: 6:221/261 of query aligns to 2:210/256 of 8w9mD

query
sites
8w9mD

I

F

L

L

Q

V

T

V

E

E

S

G

I

V

S

S

I

K

G

I

Y
|
Y

Q

P

N

T

K

P

K

E

V

G

S

P

T

Y

L

T

V

V

A

L

K

D

E

G

V

I

S

D

I

L

A

K

L

V

K

R

K

E

G

G

K

E

L

F

T

V

A

C

L

L

I

I

G

G

A
x
H

N
x
S

G
|
G

I

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

N

T

M

I

I

T

S

G

G

I

F

Q

N

T

T

P

P

I

S

E

E

G

G

D

V

V

V

L

L

H

Q

G

D

K

K

N

P

I

I

A

-

I

-

Y

-

S

-

A

-

S

T

D

E

L

P

S

G

Q

P

E

D

M

R

S

M

L

M

V

V

L

F

T
x
Q

E

N

K

Y

L

C

P

L

S

P

N

W

L

L

T

N

V

V

Y

F

E

E

L

N

V

V

A

Y

L

L

G

A

R

V

Q

D

P

-

Y

-

T

A

N

V

W

F

I

P

G

N

T

K

L

P

T

Q

E

A

E

E

D

K

K

R

I

A

Q

I

V

V

E

R

R

E

A

H

L

L

S

A

L

M

T

V

Q

G

I

L

S

T

H

E

L

A

A

A

D

E

K

K

H

P

Y

S

Q
|
Q

I

I

S
|
S

D

G

G
|
G

Q
x
M

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

S

Q

I

D

R

T

P

P

Q

L

V

I

L

I

I

L

L

D

D

E

Q

P

P

T

F

T

G

H

A

L

L

D

D

L

A

L

I

H

T

K

K

V

E

S

E

L

L

L

Q

K

E

L

E

L

L

K

L

K

Q

L

I

T

W

H

S

E

D

T

H

G

Q

K

V

C

T

I

V

L

L

F

M

S

I

T

T

H
|
H

D

D

I

I

D

D

M

E

A

A

I

L

Q

F

L

L

S

A

D

D

E

R

M

V

I

V

I

M

M

M

T

T

binding adenosine-5'-triphosphate: Y12 (= Y16), H40 (≠ A44), S41 (≠ N45), G42 (= G46), G44 (= G48), K45 (= K49), S46 (= S50), T47 (= T51), Q82 (≠ T91), Q135 (= Q146), S137 (= S148), G139 (= G150), M140 (≠ Q151), H194 (= H205)
binding magnesium ion: S46 (= S50), Q82 (≠ T91)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
34% identity, 78% coverage: 27:229/261 of query aligns to 22:227/232 of 1f3oA

query
sites
1f3oA

K

K

E

N

V

V

S

N

I

L

A

N

L

I

K

K

K

E

G

G

K

E

L

F

T

V

A

S

L

I

I

M

G

G

A

P

N

S

G
|
G

I

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

K

N

T

I

I

I

T

G

G

C

I

L

Q

D

T

K

P

P

I

T

E

E

G

G

D

E

V

V

L

Y

L

I

H

D

G

-

K

-

N

N

I

I

A

K

I

T

Y

N

S

D

A

L

S

D

D

D

L

-

S

-

Q

D

E

E

M

L

S

T

L

K

V

I

L

R

T

R

E

D

K

K

-

I

-

G

-

F

-

V

-

F

-

Q

-

F

-

N

L

L

P

I

P

P

S

L

N

-

L

L

T

T

V

A

Y

L

E

E

L

N

V

V

A

E

L

L

G

-

R

-

Q

-

P

P

Y

L

T

I

-

F

N

K

W

Y

I

R

G

G

T

A

L

M

T

S

E

G

E

E

D

E

K

R

I

R

Q

K

V

-

E

-

R

R

A

A

L

L

S

E

L

C

T

L

Q

K

I

M

S

A

H

E

L

L

-

E

-

R

-

F

A

A

D

N

K

H

K

K

H

P

Y

N

Q

Q

I

L

S

S

D

G

G

G

Q

Q

L

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

D

N

T

P

P

P

L

I

I

I

I

L

L

A

D

D

E

E

P

P

T

T

T

G

H

A

L

L

D

D

L

S

L

K

H

T

K

G

V

E

S

K

L

I

L

M

K

Q

L

L

K

K

L

L

T

N

H

E

E

E

T

D

G

G

K

K

C

T

I

V

L

V

F

V

S

V

T

T

H

H

D

D

I

I

D

N

M

V

A

A

I

-

Q

R

L

F

S

G

D

E

E

R

M

I

I

I

I

Y

M

L

T

K

P

D

E

G

T

E

V

V

V

E

Q

R

D

E

E

E

binding adenosine-5'-diphosphate: G41 (= G46), G43 (= G48), K44 (= K49), S45 (= S50), T46 (= T51)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 77% coverage: 29:228/261 of query aligns to 24:223/343 of P30750

query
sites
P30750

V

V

S

S

I

L

A

H

L

V

K

P

K

A

G

G

K

Q

L

I

T

Y

A

G

L

V

I

I

G

G

A

A

N
x
S

G
|
G

I
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

K

R

T

C

I

V

T

N

G

L

I

L

Q

E

T

R

P

P

I

T

E

E

G

G

D

S

V

V

L

L

L

V

H

D

G

G

K

Q

N

E

I

L

A

T

I

T

Y

L

S

S

A

E

S

S

D

E

L

L

S

T

-

K

-

A

-

R

Q

R

E

Q

M

I

S

G

L

M

V

I

L

F

T

Q

E

H

K

F

L

N

P

L

S

S

N

S

L

R

T

T

V

V

Y

F

E

G

L

N

V

V

A

A

L

L

G

P

R

L

Q

E

P

-

Y

-

T

-

N

-

W

L

I

D

G

N

T

T

L

P

T

K

E

D

E

E

D

V

K

K

I

R

Q

R

V

V

E

T

R

E

A

L

L

L

S

S

L

L

T

V

Q

G

I

L

S

G

H

D

L

K

A

H

D

D

K

S

K

Y

H

P

Y

S

Q

N

I

L

S

S

D

G

G

G

Q

Q

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

D

N

T

P

P

K

L

V

I

L

L

C

D

D

E
|
E

P

A

T

T

T

S

H

A

L

L

D

D

L

P

L

A

H

T

K

T

V

R

S

S

L

I

L

L

K

E

L

L

K

K

K

D

L

I

T

N

H

R

E

R

T

L

G

G

K

L

C

T

I

I

L

L

F

L

S

I

T

T

H

H

D

E

I

M

D

D

M

V

A

V

I

K

Q

R

L

I

S

C

D

D

E

C

M

V

-

A

I

V

I

I

M

S

T

N

P

G

E

E

T

L

V

I

V

E

Q

Q

D

D

40:46 (vs. 45:51, 71% identical) binding ATP
E166 (= E172) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 78% coverage: 27:229/261 of query aligns to 22:227/230 of 1l2tA

query
sites
1l2tA

K

K

E

N

V

V

S

N

I

L

A

N

L

I

K

K

K

E

G

G

K

E

L

F

T

V

A

S

L

I

I

M

G

G

A

P

N
x
S

G
|
G

I
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

K

N

T

I

I

I

T

G

G

C

I

L

Q

D

T

K

P

P

I

T

E

E

G

G

D

E

V

V

L

Y

L

I

H

D

G

-

K

-

N

N

I

I

A

K

I

T

Y

N

S

D

A

L

S

D

D

D

L

-

S

-

Q

D

E

E

M

L

S

T

L

K

V

I

L

R

T

R

E

D

K

K

-

I

-

G

-

F

-

V

-

F

-

Q

-

F

-

N

L

L

P

I

P

P

S

L

N

-

L

L

T

T

V

A

Y

L

E

E

L

N

V

V

A

E

L

L

G

-

R

-

Q

-

P

P

Y

L

T

I

-

F

N

K

W

Y

I

R

G

G

T

A

L

M

T

S

E

G

E

E

D

E

K

R

I

R

Q

K

V

-

E

-

R

R

A

A

L

L

S

E

L

C

T

L

Q

K

I

M

S

A

H

E

L

L

-

E

-

R

-
x
F

A

A

D

N

K

H

K

K

H

P

Y

N

Q
|
Q

I

L

S
|
S

D

G

G
|
G

Q
|
Q

L

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

D

N

T

P

P

P

L

I

I

I

I

L

L

A

D

D

E

Q

P

P

T

T

T

G

H

A

L

L

D

D

L

S

L

K

H

T

K

G

V

E

S

K

L

I

L

M

K

Q

L

L

K

K

L

L

T

N

H

E

E

E

T

D

G

G

K

K

C

T

I

V

L

V

F

V

S

V

T

T

H
|
H

D

D

I

I

D

N

M

V

A

A

I

-

Q

R

L

F

S

G

D

E

E

R

M

I

I

I

I

Y

M

L

T

K

P

D

E

G

T

E

V

V

V

E

Q

R

D

E

E

E

binding adenosine-5'-triphosphate: S40 (≠ N45), G41 (= G46), S42 (≠ I47), G43 (= G48), K44 (= K49), S45 (= S50), T46 (= T51), F138 (vs. gap), Q145 (= Q146), S147 (= S148), G149 (= G150), Q150 (= Q151), H204 (= H205)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
35% identity, 76% coverage: 27:224/261 of query aligns to 24:216/658 of 8wm7C

query
sites
8wm7C

K

K

E

N

V

I

S

E

I

L

A

K

L

I

K

K

K

Q

G

G

K

E

L

F

T

V

A

S

L

L

I

I

G

G

A

H

N

S

G
|
G

I
x
C

G
|
G

K

K

S
|
S

T

T

L

L

K

N

T

I

I

I

T

A

G

G

I

L

Q

D

T

R

P

A

I

S

E

I

G

G

D

G

V

V

L

T

L

L

H

E

G

G

K

R

N

E

I

I

A

R

I

E

Y

P

S

S

A

P

S

D

D

-

L

-

S

-

Q

-

E

R

M

M

S

V

L

V

V

F

L

Q

T

N

E

Y

K

S

L

L

P

L

P

P

S

W

N

-

L

L

T

T

V

V

Y

R

E

E

L

N

V

V

A

A

L

L

G

A

R

V

Q

D

P

E

Y

V

T

-

N

-

W

Y

I

Q

G

G

T

K

L

S

T

K

E

G

E

E

D

R

K

R

I

A

Q

I

V

I

E

E

R

E

A

H

L

I

S

D

L

M

T

V

Q

G

I

L

S

R

H

L

L

A

A

A

D

N

K

K

K

R

H

P

Y

S

Q

E

I

L

S

S

D

G

G

G

Q

M

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

T

D

R

T

P

P

K

L

L

I

L

L

L

D

D

E

E

P

P

T

F

T

G

H

A

L

L

D

D

L

A

L

L

H

T

K

R

V

G

S

S

L

L

L

Q

K

E

L

Q

L

L

K

M

K

K

L

I

T

C

H

N

E

E

T

H

G

Q

K

I

C

T

I

C

L

V

F

M

S

V

T

T

H

H

D

D

I

V

D

D

M

E

A

A

I

L

Q

L

L

L

S

S

D

D

E

R

M

V

I

V

I

M

M

L

T

T

-

N

-

G

P

P

E

E

T

A

binding adenosine-5'-diphosphate: G43 (= G46), C44 (≠ I47), G45 (= G48), S47 (= S50)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 20

7e7oA Cryo-em structure of human abca4 in nrpe-bound state (see paper)
30% identity, 77% coverage: 19:220/261 of query aligns to 818:1012/2003 of 7e7oA

query
sites
7e7oA

K

K

K

N

V

L

S

V

T

K

L

I

V

A

A

V

K

D

E

R

V

L

S

N

I

I

A

T

L

F

K

Y

K

E

G

N

K

Q

L

I

T

T

A

A

L

F

I

L

G

G

A

H

N

N

G

G

I

A

G

G

K

K

S

T

T

T

L

T

L

L

K

S

T

I

I

L

T

T

G

G

I

L

Q

L

T

P

P

P

I

T

E

S

G

G

D

T

V

V

L

L

L

V

H

G

G

G

K

R

N

D

I

I

A

E

I

T

Y

-

S

S

A

L

S

D

D

A

L

V

S

R

Q

Q

E

S

M

L

S

G

L

M

V

C

L

P

T

Q

E

H

K

N

L

I

P

L

P

F

S

H

N

H

L

L

T

T

V

V

Y

A

E

E

L

H

V

M

A

L

L

F

G

-

R

-

Q

-

P

-

Y

Y

T

A

N

Q

W

L

I

K

G

G

T

K

L

S

T

Q

E

E

D

A

K

Q

I

L

Q

E

V

M

E

E

R

A

A

M

L

L

S

E

L

D

T

T

Q

G

I

L

S

H

H

H

L

K

A

R

D

N

K

E

H

A

Y

Q

Q

D

I

L

S

S

D

G

G

G

Q

M

L

Q

Q

R

K

K

V

L

L

S

I

V

A

A

R

I

A

A

L

F

A

V

Q

G

D

D

T

A

P

K

L

V

I

V

I

I

L

L

D

D

E

E

P

P

T

T

T

S

H

G

L

V

D

D

L

P

L

Y

H

S

K

R

V

R

S

S

L

I

L

W

K

D

L

L

K

-

L

L

T

K

H

Y

E

R

T

S

G

G

K

R

C

T

I

I

L

I

F

M

S

S

T

T

H

H

D

H

I

M

D

D

M

E

A

A

I

D

Q

L

L

L

S

G

D

D

E

R

M

I

I

A

I

I

M

I

Sites not aligning to the query:

binding [(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate: 285, 290, 526, 527, 589, 592, 1454

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 77% coverage: 29:228/261 of query aligns to 25:224/344 of 3tuzC

query
sites
3tuzC

V

V

S

S

I

L

A

H

L

V

K

P

K

A

G

G

K

Q

L

I

T

Y

A

G

L

V

I

I

G

G

A

A

N

S

G
|
G

I

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

K

R

T

C

I

V

T

N

G

L

I

L

Q

E

T

R

P

P

I

T

E

E

G

G

D

S

V

V

L

L

L

V

H

D

G

G

K

Q

N

E

I

L

A

T

I

T

Y

L

S

S

A

E

S

S

D

E

L

L

S

T

-

K

-

A

-

R

Q

R

E

Q

M

I

S

G

L

M

V

I

L

F

T

Q

E

H

K

F

L

N

P

L

S

S

N

S

L

R

T

T

V

V

Y

F

E

G

L

N

V

V

A

A

L

L

G

P

R

L

Q

E

P

-

Y

-

T

-

N

-

W

L

I

D

G

N

T

T

L

P

T

K

E

D

E

E

D

V

K

K

I

R

Q

R

V

V

E

T

R

E

A

L

L

L

S

S

L

L

T

V

Q

G

I

L

S

G

H

D

L

K

A

H

D

D

K

S

K

Y

H

P

Y

S

Q

N

I

L

S

S

D

G

G

G

Q

Q

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

D

N

T

P

P

K

L

V

I

L

L

C

D

D

E

Q

P

A

T

T

T

S

H

A

L

L

D

D

L

P

L

A

H

T

K

T

V

R

S

S

L

I

L

L

K

E

L

L

K

K

K

D

L

I

T

N

H

R

E

R

T

L

G

G

K

L

C

T

I

I

L

L

F

L

S

I

T

T

H

H

D

E

I

M

D

D

M

V

A

V

I

K

Q

R

L

I

S

C

D

D

E

C

M

V

-

A

I

V

I

I

M

S

T

N

P

G

E

E

T

L

V

I

V

E

Q

Q

D

D

binding adenosine-5'-diphosphate: G42 (= G46), G44 (= G48), K45 (= K49), S46 (= S50), T47 (= T51)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 77% coverage: 29:228/261 of query aligns to 25:224/344 of 3tuiC

query
sites
3tuiC

V

V

S

S

I

L

A

H

L

V

K

P

K

A

G

G

K

Q

L

I

T

Y

A

G

L

V

I

I

G

G

A

A

N

S

G
|
G

I
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

K

R

T

C

I

V

T

N

G

L

I

L

Q

E

T

R

P

P

I

T

E

E

G

G

D

S

V

V

L

L

L

V

H

D

G

G

K

Q

N

E

I

L

A

T

I

T

Y

L

S

S

A

E

S

S

D

E

L

L

S

T

-

K

-

A

-

R

Q

R

E

Q

M

I

S

G

L

M

V

I

L

F

T

Q

E

H

K

F

L

N

P

L

S

S

N

S

L

R

T

T

V

V

Y

F

E

G

L

N

V

V

A

A

L

L

G

P

R

L

Q

E

P

-

Y

-

T

-

N

-

W

L

I

D

G

N

T

T

L

P

T

K

E

D

E

E

D

V

K

K

I

R

Q

R

V

V

E

T

R

E

A

L

L

L

S

S

L

L

T

V

Q

G

I

L

S

G

H

D

L

K

A

H

D

D

K

S

K

Y

H

P

Y

S

Q

N

I

L

S

S

D

G

G

G

Q

Q

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

D

N

T

P

P

K

L

V

I

L

L

C

D

D

E

Q

P

A

T

T

T

S

H

A

L

L

D

D

L

P

L

A

H

T

K

T

V

R

S

S

L

I

L

L

K

E

L

L

K

K

K

D

L

I

T

N

H

R

E

R

T

L

G

G

K

L

C

T

I

I

L

L

F

L

S

I

T

T

H

H

D

E

I

M

D

D

M

V

A

V

I

K

Q

R

L

I

S

C

D

D

E

C

M

V

-

A

I

V

I

I

M

S

T

N

P

G

E

E

T

L

V

I

V

E

Q

Q

D

D

binding adenosine-5'-diphosphate: G42 (= G46), A43 (≠ I47), G44 (= G48), K45 (= K49), S46 (= S50), T47 (= T51)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

8w9mC Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
35% identity, 76% coverage: 27:224/261 of query aligns to 24:216/256 of 8w9mC

query
sites
8w9mC

K

K

E

N

V

I

S

E

I

L

A

K

L

I

K

K

K

Q

G

G

K

E

L

F

T

V

A

S

L

L

I

I

G

G

A

H

N
x
S

G
|
G

I

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

N

T

I

I

I

T

A

G

G

I

L

Q

D

T

R

P

A

I

S

E

I

G

G

D

G

V

V

L

T

L

L

H

E

G

G

K

R

N

E

I

I

A

R

I

E

Y

P

S

S

A

P

S

D

D

-

L

-

S

-

Q

-

E

R

M

M

S

V

L

V

V

F

L
x
Q

T

N

E

Y

K

S

L

L

P

L

P

P

S

W

N

-

L

L

T

T

V

V

Y

R

E

E

L

N

V

V

A

A

L

L

G

A

R

V

Q

D

P

E

Y

V

T

-

N

-

W

Y

I

Q

G

G

T

K

L

S

T

K

E

G

E

E

D

R

K

R

I

A

Q

I

V

I

E

E

R

E

A

H

L

I

S

D

L

M

T

V

Q

G

I

L

S

R

H

L

L

A

A

A

D

N

K
|
K

K

R

H

P

Y

S

Q
x
E

I

L

S
|
S

D

G

G
|
G

Q

M

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

T

D

R

T

P

P

K

L

L

I

L

L

L

D

D

E

Q

P

P

T

F

T

G

H

A

L

L

D

D

L

A

L

L

H

T

K

R

V

G

S

S

L

L

L

Q

K

E

L

Q

L

L

K

M

K

K

L

I

T

C

H

N

E

E

T

H

G

Q

K

I

C

T

I

C

L

V

F

M

S

V

T

T

H
|
H

D

D

I

V

D

D

M

E

A

A

I

L

Q

L

L

L

S

S

D

D

E

R

M

V

I

V

I

M

M

L

T

T

-

N

-

G

P

P

E

E

T

A

binding adenosine-5'-triphosphate: S42 (≠ N45), G43 (= G46), G45 (= G48), K46 (= K49), S47 (= S50), T48 (= T51), Q83 (≠ L90), K132 (= K142), E136 (≠ Q146), S138 (= S148), G140 (= G150), H195 (= H205)
binding magnesium ion: S47 (= S50), Q83 (≠ L90)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 20

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 77% coverage: 29:228/261 of query aligns to 25:224/344 of 6cvlD

query
sites
6cvlD

V

V

S

S

I

L

A

H

L

V

K

P

K

A

G

G

K

Q

L

I

T

Y

A

G

L

V

I

I

G

G

A

A

N
x
S

G
|
G

I
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

K

R

T

C

I

V

T

N

G

L

I

L

Q

E

T

R

P

P

I

T

E

E

G

G

D

S

V

V

L

L

L

V

H

D

G

G

K

Q

N

E

I

L

A

T

I

T

Y

L

S

S

A

E

S

S

D

E

L

L

S

T

-

K

-

A

-

R

Q

R

E

Q

M

I

S

G

L

M

V

I

L

F

T

Q

E

H

K

F

L

N

P

L

S

S

N

S

L

R

T

T

V

V

Y

F

E

G

L

N

V

V

A

A

L

L

G

P

R

L

Q

E

P

-

Y

-

T

-

N

-

W

L

I

D

G

N

T

T

L

P

T

K

E

D

E

E

D

V

K

K

I

R

Q

R

V

V

E

T

R

E

A

L

L

L

S

S

L

L

T

V

Q

G

I

L

S

G

H

D

L

K

A

H

D

D

K

S

K

Y

H

P

Y

S

Q
x
N

I

L

S
|
S

D

G

G

G

Q
|
Q

L

K

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

S

D

N

T

P

P

K

L

V

I

L

L

C

D

D

E

Q

P

A

T

T

T

S

H

A

L

L

D

D

L

P

L

A

H

T

K

T

V

R

S

S

L

I

L

L

K

E

L

L

K

K

K

D

L

I

T

N

H

R

E

R

T

L

G

G

K

L

C

T

I

I

L

L

F

L

S

I

T

T

H
|
H

D

E

I

M

D

D

M

V

A

V

I

K

Q

R

L

I

S

C

D

D

E

C

M

V

-

A

I

V

I

I

M

S

T

N

P

G

E

E

T

L

V

I

V

E

Q

Q

D

D

binding phosphothiophosphoric acid-adenylate ester: S41 (≠ N45), G42 (= G46), A43 (≠ I47), G44 (= G48), K45 (= K49), S46 (= S50), T47 (= T51), N141 (≠ Q146), S143 (= S148), Q146 (= Q151), H200 (= H205)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
27% identity, 79% coverage: 23:227/261 of query aligns to 13:211/367 of 1q12A

query
sites
1q12A

T

V

L

V

V

V

A

S

K

K

E

D

V

I

S

N

I

L

A

D

L

I

K

H

K

E

G

G

K

E

L

F

T

V

A

V

L

F

I

V

G

G

A

P

N
x
S

G
|
G

I
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

T

M

I

I

T

A

G

G

I

L

Q

E

T

T

P

I

I

T

E

S

G

G

D

D

V

L

L

F

L

I

H

G

G

E

K

K

N

R

I

M

A

N

I

D

Y

T

S

P

A

P

S

A

D

E

L

R

S

G

Q

V

E

G

M

M

S

V

L

F

V

Q

L

S

T

Y

E

A

K

L

L

Y

P

P

P

-

S

-

N

H

L

L

T

S

V

V

Y

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

I

A

G

G

T

A

L

K

T

K

E

E

E

V

D

I

K

N

I

Q

Q

R

V

V

E

N

R

Q

A

V

L

A

S

E

L

V

T

L

Q

Q

I

L

S

A

H

H

L

L

A

L

D

D

K
x
R

K

K

H

P

Y

K

Q
x
A

I

L

S
|
S

D

G

G
|
G

Q
|
Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

T

P

P

S

L

V

I

F

I

L

L

L

D

D

E

E

P

P

T

L

T

S

H

N

L

L

D

D

L

A

H

L

K

R

V

V

S

Q

L

M

L

R

K

I

L

E

L

I

K

S

K

R

L

L

T

H

H

K

E

R

T

L

G

G

K

R

C

T

I

M

L

I

F

Y

S

V

T

T

H

H

D

D

I

Q

D

V

M

E

A

A

I

M

Q

T

L

L

S

A

D

D

E

K

M

I

I

V

M

L

T

D

P

A

E

G

T

R

V

V

V

A

Q

Q

binding adenosine-5'-triphosphate: S35 (≠ N45), G36 (= G46), C37 (≠ I47), G38 (= G48), K39 (= K49), S40 (= S50), T41 (= T51), R126 (≠ K142), A130 (≠ Q146), S132 (= S148), G134 (= G150), Q135 (= Q151)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

Query Sequence

>WP_078211275.1 NCBI__GCF_002017945.1:WP_078211275.1
MEHTIILQTESISIGYQNKKVSTLVAKEVSIALKKGKLTALIGANGIGKSTLLKTITGIQ
TPIEGDVLLHGKNIAIYSASDLSQEMSLVLTEKLPPSNLTVYELVALGRQPYTNWIGTLT
EEDKIQVERALSLTQISHLADKKHYQISDGQLQKVLIARALAQDTPLIILDEPTTHLDLL
HKVSLLKLLKKLTHETGKCILFSTHDIDMAIQLSDEMIIMTPETVVQDEPCNLIAKGSFN
TLFKDEHIVFDAEKGKFVITN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory