SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078212752.1 NCBI__GCF_002017945.1:WP_078212752.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P0AC98 Succinate-acetate/proton symporter SatP; Succinate-acetate transporter protein from Escherichia coli (strain K12) (see paper)
55% identity, 99% coverage: 2:184/185 of query aligns to 3:183/188 of P0AC98

query
sites
P0AC98

E

N

K

T

K

K

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L

A

A

N

N

V

P

A

A

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P

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P

L131 (= L132) mutation to V: Acquires ability to transport lactic acid.
A164 (= A165) mutation to G: Acquires ability to transport lactic acid.

5ys3A 1.8 angstrom crystal structure of succinate-acetate permease from citrobacter koseri (see paper)
55% identity, 98% coverage: 4:184/185 of query aligns to 1:179/188 of 5ys3A

query
sites
5ys3A

K

K

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binding (2s)-2,3-dihydroxypropyl(7z)-pentadec-7-enoate: G103 (= G108), A132 (≠ F137), I139 (≠ F144)

5ys8A 2.8 angstrom crystal structure of succinate-acetate permease from citrobacter koseri (see paper)
55% identity, 97% coverage: 6:184/185 of query aligns to 2:178/182 of 5ys8A

query
sites
5ys8A

A

A

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N

V

P

A

A

P

P

L

L

G

G

L

L

L

M

G

G

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P

binding acetate ion: T16 (= T20), F59 (= F63), F64 (= F68), F70 (= F74), W71 (= W75), F123 (= F129), F130 (= F136)

P41943 Glyoxylate pathway regulator from Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) (see paper)
31% identity, 96% coverage: 2:179/185 of query aligns to 74:255/270 of P41943

query
sites
P41943

E
x
S

K

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K

K

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F

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G

N

N

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P

A

A

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P

L

L

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G

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F

G

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G

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S

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Y

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G

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F

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W

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S

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A

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S

S74 (≠ E2) mutation S->A,Q: No effect on sensitivity of cells to acetic acid.; mutation S->H,D: No effect on sensitivity of cells to acetic acid.

Sites not aligning to the query:

58 mutation Y->E,F: No effect on sensitivity of cells to acetic acid.

Query Sequence

>WP_078212752.1 NCBI__GCF_002017945.1:WP_078212752.1
MEKKQANVAPLGLLGFGMTTILLNIHNMGFFPVSSVIISMGLFYGGMAQIIAGIFSLHQG
KTFAGTAFTSYGFFWISLVGIWLFPNTGFELAGTTPAPFFGCYLVLWGLFTAFMWWGTWG
GSKVQQFVFLSLTILFFVLALSKFLNSEAINEIAGVIGVICGGSAFYLAMAELLEEVKSS
KVLPY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory