SitesBLAST
Comparing WP_078212859.1 NCBI__GCF_002017945.1:WP_078212859.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 13 hits to proteins with known functional sites (download)
5ur2B Crystal structure of proline utilization a (puta) from bdellovibrio bacteriovorus inactivated by n-propargylglycine (see paper)
28% identity, 53% coverage: 161:367/391 of query aligns to 196:401/959 of 5ur2B
- binding N-propargylglycine-modified flavin adenine dinucleotide: D215 (= D180), M216 (≠ A181), Q249 (= Q213), V278 (= V242), K279 (≠ R243), G280 (= G244), A281 (= A245), W283 (≠ M247), Y300 (≠ C264), T301 (≠ A265), N302 (≠ S266), K303 (= K267), S306 (≠ T270), A329 (≠ G293), S330 (≠ T294), H331 (= H295), N332 (= N296), Q356 (≠ G322), M357 (≠ Q323), L358 (= L324), Y379 (= Y345), E398 (= E364)
Sites not aligning to the query:
4nmaA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca in complex with l-tetrahydro-2-furoic acid (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 205:410/977 of 4nmaA
- binding flavin-adenine dinucleotide: D226 (= D180), M227 (≠ A181), Q258 (= Q213), R285 (≠ K240), V287 (= V242), K288 (≠ R243), G289 (= G244), A290 (= A245), Y291 (= Y246), W292 (≠ M247), W309 (≠ C264), T310 (≠ A265), I311 (≠ S266), K312 (= K267), S315 (≠ T270), A338 (≠ G293), S339 (≠ T294), H340 (= H295), N341 (= N296), Q365 (≠ G322), L367 (= L324), E407 (= E364)
- binding tetrahydrofuran-2-carboxylic acid: Y388 (= Y345), Y400 (= Y357), R403 (= R360), R404 (= R361)
Sites not aligning to the query:
4nmfA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 201:406/973 of 4nmfA
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: Y287 (= Y246), Y384 (= Y345), Y396 (= Y357), R399 (= R360), R400 (= R361)
- binding N-propargylglycine-modified flavin adenine dinucleotide: D222 (= D180), M223 (≠ A181), V252 (≠ T211), Q254 (= Q213), R281 (≠ K240), V283 (= V242), K284 (≠ R243), G285 (= G244), A286 (= A245), W288 (≠ M247), W305 (≠ C264), T306 (≠ A265), I307 (≠ S266), K308 (= K267), S311 (≠ T270), A334 (≠ G293), S335 (≠ T294), H336 (= H295), N337 (= N296), Q361 (≠ G322), V362 (≠ Q323), L363 (= L324), Y384 (= Y345), E403 (= E364)
Sites not aligning to the query:
- active site: 625, 648, 729, 763, 859, 939
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: 181
- binding N-propargylglycine-modified flavin adenine dinucleotide: 181, 408, 410
4nmdA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca reduced with dithionite (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 205:410/979 of 4nmdA
- binding dihydroflavine-adenine dinucleotide: D226 (= D180), M227 (≠ A181), V256 (≠ T211), Q258 (= Q213), R285 (≠ K240), V287 (= V242), K288 (≠ R243), G289 (= G244), A290 (= A245), W292 (≠ M247), W309 (≠ C264), T310 (≠ A265), I311 (≠ S266), K312 (= K267), S315 (≠ T270), A338 (≠ G293), S339 (≠ T294), H340 (= H295), N341 (= N296), Q365 (≠ G322), V366 (≠ Q323), L367 (= L324), Y388 (= Y345)
Sites not aligning to the query:
4nmfB Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 204:409/979 of 4nmfB
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: Y290 (= Y246), Y387 (= Y345), Y399 (= Y357), R402 (= R360), R403 (= R361)
- binding (2S)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: L366 (= L324), Y399 (= Y357), R402 (= R360)
- binding N-propargylglycine-modified flavin adenine dinucleotide: D225 (= D180), M226 (≠ A181), V255 (≠ T211), Q257 (= Q213), R284 (≠ K240), V286 (= V242), K287 (≠ R243), G288 (= G244), A289 (= A245), W291 (≠ M247), W308 (≠ C264), T309 (≠ A265), I310 (≠ S266), K311 (= K267), S314 (≠ T270), A337 (≠ G293), S338 (≠ T294), H339 (= H295), N340 (= N296), Q364 (≠ G322), L366 (= L324), Y387 (= Y345), E406 (= E364)
Sites not aligning to the query:
- active site: 631, 654, 735, 769, 865, 945
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: 184
- binding (2S)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: 184
- binding N-propargylglycine-modified flavin adenine dinucleotide: 184, 411, 412, 413
7na0A Structure of geobacter sulfurreducens proline utilization a (puta) variant a206w (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 205:410/981 of 7na0A
- binding flavin-adenine dinucleotide: D226 (= D180), M227 (≠ A181), V256 (≠ T211), Q258 (= Q213), R285 (≠ K240), V287 (= V242), K288 (≠ R243), G289 (= G244), A290 (= A245), Y291 (= Y246), W292 (≠ M247), W309 (≠ C264), T310 (≠ A265), I311 (≠ S266), K312 (= K267), S315 (≠ T270), A338 (≠ G293), S339 (≠ T294), H340 (= H295), N341 (= N296), L367 (= L324), Y388 (= Y345), E407 (= E364)
Sites not aligning to the query:
6bsnA Structure of proline utilization a (puta) with proline bound in remote sites (see paper)
25% identity, 54% coverage: 158:367/391 of query aligns to 247:454/973 of 6bsnA
- binding dihydroflavine-adenine dinucleotide: D269 (= D180), A270 (= A181), Q303 (= Q213), R330 (≠ K240), V332 (= V242), K333 (≠ R243), G334 (= G244), A335 (= A245), Y336 (= Y246), W337 (≠ M247), F355 (≠ C264), T356 (≠ A265), R357 (≠ S266), K358 (= K267), T361 (= T270), A384 (= A292), T385 (= T294), H386 (= H295), N387 (= N296), Y432 (= Y345)
Sites not aligning to the query:
- active site: 643, 743, 777, 951
- binding dihydroflavine-adenine dinucleotide: 457, 458
- binding proline: 630, 642, 644, 718, 776, 778, 871, 930, 931, 932, 939, 958, 959, 961
3hazA Crystal structure of bifunctional proline utilization a (puta) protein (see paper)
25% identity, 54% coverage: 158:367/391 of query aligns to 250:457/983 of 3hazA
- binding flavin-adenine dinucleotide: D272 (= D180), A273 (= A181), Q306 (= Q213), R333 (≠ K240), V335 (= V242), K336 (≠ R243), G337 (= G244), A338 (= A245), Y339 (= Y246), W340 (≠ M247), F358 (≠ C264), T359 (≠ A265), R360 (≠ S266), K361 (= K267), T364 (= T270), A387 (= A292), T388 (= T294), H389 (= H295), N390 (= N296), Y435 (= Y345)
Sites not aligning to the query:
- active site: 652, 675, 752, 786, 878, 960
- binding flavin-adenine dinucleotide: 460, 461
- binding nicotinamide-adenine-dinucleotide: 648, 649, 650, 651, 652, 657, 675, 676, 677, 708, 711, 712, 726, 727, 728, 731, 735, 752, 753, 786, 878, 880, 948
4nmeA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine (see paper)
25% identity, 53% coverage: 159:367/391 of query aligns to 203:405/972 of 4nmeA
- binding N-propargylglycine-modified flavin adenine dinucleotide: D224 (= D180), M225 (≠ A181), V254 (≠ T211), Q256 (= Q213), R283 (≠ K240), V285 (= V242), K286 (≠ R243), G287 (= G244), A288 (= A245), W290 (≠ M247), W307 (≠ C264), T308 (≠ A265), I309 (≠ S266), K310 (= K267), S313 (≠ T270), A336 (≠ G293), S337 (≠ T294), H338 (= H295), N339 (= N296), Q363 (≠ G322), L365 (= L324), Y383 (= Y345), E402 (= E364)
Sites not aligning to the query:
4nmcA Crystal structure of oxidized proline utilization a (puta) from geobacter sulfurreducens pca complexed with zwittergent 3-12 (see paper)
24% identity, 53% coverage: 159:364/391 of query aligns to 194:396/941 of 4nmcA
- binding flavin-adenine dinucleotide: D215 (= D180), M216 (≠ A181), V245 (≠ T211), Q247 (= Q213), R274 (≠ K240), V276 (= V242), K277 (≠ R243), G278 (= G244), A279 (= A245), W281 (≠ M247), W298 (≠ C264), T299 (≠ A265), I300 (≠ S266), K301 (= K267), S304 (≠ T270), A327 (≠ G293), S328 (≠ T294), H329 (= H295), N330 (= N296), L356 (= L324), Y377 (= Y345)
Sites not aligning to the query:
Q9RW55 Proline dehydrogenase; PRODH; DrPRODH; Proline oxidase; EC 1.5.5.2 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see paper)
24% identity, 80% coverage: 53:365/391 of query aligns to 19:296/310 of Q9RW55
- G63 (≠ V94) mutation to A: 140-fold decrease in catalytic efficiency for proline.
- E64 (= E95) mutation to A: 27-fold decrease in catalytic efficiency for proline.
- K98 (= K129) binding substrate
- M136 (≠ A181) binding FAD
- Q166 (= Q213) binding FAD
- RMVKGA 187:192 (≠ KLVRGA 240:245) binding FAD
- TH 229:230 (≠ TY 300:301) binding FAD
- RR 291:292 (= RR 360:361) binding substrate
- RIAE 292:295 (≠ RAEE 361:364) binding FAD
4h6qA Structure of oxidized deinococcus radiodurans proline dehydrogenase complexed with l-tetrahydrofuroic acid (see paper)
24% identity, 80% coverage: 53:365/391 of query aligns to 3:280/281 of 4h6qA
- binding flavin-adenine dinucleotide: D119 (= D180), M120 (≠ A181), Q150 (= Q213), R171 (≠ K240), V173 (= V242), K174 (≠ R243), G175 (= G244), A176 (= A245), A212 (≠ S299), T213 (= T300), H214 (≠ Y301), M240 (≠ Q323), L241 (= L324), I244 (≠ M327), R276 (= R361), E279 (= E364)
- binding tetrahydrofuran-2-carboxylic acid: K82 (= K129), Y262 (= Y345), R275 (= R360), R276 (= R361)
Sites not aligning to the query:
4h6rA Structure of reduced deinococcus radiodurans proline dehydrogenase (see paper)
23% identity, 77% coverage: 63:365/391 of query aligns to 7:271/272 of 4h6rA
- binding dihydroflavine-adenine dinucleotide: D110 (= D180), M111 (≠ A181), V139 (≠ T211), Q141 (= Q213), R162 (≠ K240), V164 (= V242), K165 (≠ R243), G166 (= G244), A167 (= A245), A203 (≠ S299), T204 (= T300), H205 (≠ Y301), Q230 (≠ G322), L232 (= L324), I235 (≠ M327), Y253 (= Y345), R267 (= R361), E270 (= E364)
Sites not aligning to the query:
Query Sequence
>WP_078212859.1 NCBI__GCF_002017945.1:WP_078212859.1
MNNLFNNTQVAFALKSDTELDRAYFLFKMIASEPLVRIGTAVTNFAIKANLPVESLIRAT
VFDHFCGGINENDCLSVVDKMFTKGVSSVLDYSVEGKEEEAQFDAALKMTLKTIEFAKER
LAIPFAVFKPTGLGRLALYEKLGEKKELTAEETIEWNKIVERFDTVCKEAYIKDVALLID
AEESWMQDVADELVTTMMRKYNKEKAIIFNTLQMYRWDRLDYLKKLHEEAKRDQFFIGMK
LVRGAYMEKENLRAQEMNYPTPICASKEATDANYDAAVTYMMEHLEFMSIFAGTHNELST
YKLMDLMEKNGVKNNDPRIYFGQLYGMSDNISYNLAENGYNVAKYLPFGPVKDVMPYLIR
RAEENTSVAGQTSRELLLIKTERKRRKEKNA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory