SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078427294.1 NCBI__GCF_002019605.1:WP_078427294.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
45% identity, 96% coverage: 2:501/520 of query aligns to 3:502/517 of Q9JZG1

query
sites
Q9JZG1

K

Q

K

T

D

N

Q

R

V

V

I

I

L

I

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q

Q

T

S

P

P

G

G

V

A

N

A

L

M

N

T

E

K

N

E

D

E

K

K

V

I

K

R

I

V

A

A

L

R

R

Q

L

L

E

E

K

K

L

L

G

G

I

V

D

D

I

I

E

E

A

A

G

G

F

F

A

A

S

S

P

P

G

G

D

D

A

F

K

E

A

A

V

V

R

N

A

A

V

I

A

A

E

K

A

T

V

I

H

T

K

K

P

S

L

T

V

V

V

C

S

S

L

L

A

S

R

R

T

A

N

I

K

E

N

R

D

D

I

I

D

R

E

Q

V

A

V

G

K

E

A

A

F

V

K

A

G

P

I

A

T

P

N

K

V

K

G

R

I

I

H

H

I

T

F

F

I

I

A

A

T

T

S

S

P

P

I

I

H

H

R

M

E

E

R

Y

K

K

L

L

G

K

M

M

T

K

K

P

Q

K

E

Q

V

V

V

I

D

E

K

A

A

A

V

V

E

K

S

A

V

V

A

K

Y

I

A

A

S

R

Q

E

F

Y

F

T

D

D

H

D

I

V

E

E

F

F

S

S

C

C

E

E

D

D

S

A

T

L

R

R

T

S

E

E

L

I

E

D

F

F

L

L

R

A

E

E

I

I

S

C

E

G

K

A

V

V

I

I

E

E

A

A

G

G

A

A

T

T

V

T

L

I

N

N

F

I

P

P

D

D

T

T

V

V

G

G

Y

Y

T

S

T

I

P

P

E

Y

E

K

Y

T

T

E

E

E

L

F

F

F

Q

R

Y

E

I

L

L

I

N

A

H

K

T

T

R

P

G

N

I

G

E

G

K

K

V

V

I

V

L

W

S

S

C

A

H
|
H

C

C

H
|
H

D

N

D

D

L

L

G

G

L

L

A

A

L

V

S

A

N

N

S

S

I

L

A

A

M

L

K

K

A

G

G

G

A

A

L

R

Q

Q

I

V

E

E

G

C

C

T

I

V

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N
|
N

V

A

A

S

L

V

E

E

V

I

A

V

A

M

L

A

I

L

E

K

T

V

R

R

S

H

E

D

H

L

Y

F

Q

G

R

L

K

E

T

T

G

G

I

I

N

D

L

T

K

T

E

Q

I

I

A

V

K

P

T

S

S

S

N

K

L

L

V

V

S

S

R

T

L

I

T

T

G

G

I

Y

S

P

I

V

P

Q

P

P

N

N

K

K

A

A

V

I

V

V

G

G

G

A

N

N

A

A

Y

F

A

S

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

V

I

L

K

K

D

H

K

R

S

E

T

T

Y

Y

E

E

I

I

L

M

K

S

P

A

E

E

S

S

V

V

G

G

F

W

S

A

E

T

T

N

K

R

L

L

V

S

L

L

G

G

K

K

L

L

S

S

G

G

R

R

H

N

A

A

L

F

K

K

K

T

R

K

Y

L

E

A

E

D

L

L

G

G

Y

I

H

E

V

L

D

E

D

S

Q

E

-

A

L

L

K

N

E

A

I

A

F

F

T

A

K

R

Y

F

K

K

E

E

L
|
L

T
x
A

D
|
D

R
x
K

K
|
K

K
x
R

E
|
E

I
|
I

F
|
F

D
|
D

N
x
E

D
|
D

I
x
L

I
x
H

A
|
A

L
|
L

L
x
V

E
x
S

S
x
D

E
|
E

Q
x
M

P
x
G

N
x
S

E
x
M

G
x
N

T
x
A

N
x
E

Y
x
S

L
x
Y

S
x
K

F
|
F

-
x
I

-
x
S

Q
|
Q

D
x
K

V
x
I

S
|
S

L
x
T

Q
x
E

V
x
T

L
x
G

N
x
E

K
x
P

Q
x
R

Y
x
A

R
x
D

A
x
I

F
x
V

V
x
F

N
x
S

I
|
I

N
x
K

G
|
G

K
x
E

D
x
E

Q
x
K

Q
x
R

V
x
A

I

-

E

-

G

-

I
x
S

A
|
A

K
x
T

G
|
G

N
x
S

G
|
G

P
|
P

I
x
V

D
|
D

A
|
A

I
|
I

F
|
F

N
x
K

A
|
A

I
|
I

D
x
E

T
x
S

L
x
V

I
x
A

N
x
Q

L
x
S

D
x
G

V
x
A

E
x
A

L
|
L

V
x
Q

D
x
I

Y
|
Y

K
x
S

I
x
V

T
x
N

S
x
A

V
|
V

S
x
T

Q
|
Q

G
|
G

K
x
T

D
x
E

S
|
S

L
x
Q

G
|
G

E
|
E

V
x
T

F
x
S

V
|
V

K
x
R

V
x
L

S
x
A

S
x
R

G
|
G

N
|
N

S
x
R

I
x
V

F
x
V

Q
x
N

G
|
G

R
x
Q

G
|
G

M
x
A

D
|
D

V
x
T

D
|
D

I
x
V

I
x
L

V
|
V

A
|
A

S
x
T

T
x
A

T
x
K

A
|
A

Y
|
Y

I
x
L

D
x
S

A
|
A

I
x
L

N
x
S

R
x
K

I
x
L

D16 (= D15) binding Mn(2+)
H204 (= H203) binding Mn(2+)
H206 (= H205) binding Mn(2+)
N240 (= N239) binding Mn(2+)

Sites not aligning to the query:

366:517 Required for the condensation reaction. Not required to bind substrate

Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 73% coverage: 3:380/520 of query aligns to 82:474/503 of Q9FN52

query
sites
Q9FN52

K

K

D

N

Q

Y

V

V

I

R

L

V

F

L

D

D

T

T

L

L

R

R

D

D

G

G

E

E

Q

Q

T

S

P

P

G

G

V

A

N

A

L

L

N

T

E

P

N

P

D

Q

K

K

V

L

K

E

I

I

A

A

L

R

R

Q

L

L

E

A

K

K

L

L

G

R

I

V

D

D

I

I

I

M

E

E

A

V

G

G

F

F

A

P

A

V

A

S

S

S

P

E

G

E

D

E

A

F

K

E

A

A

V

I

R

K

A

T

V

I

A

A

E

K

A

T

V

V

-

G

-

N

-

E

-

V

-

D

-

E

-

T

-

G

H

Y

K

V

P

P

L

V

V

I

V

C

S

G

L

I

A

A

R

R

T

C

N

K

K

K

N

R

D

D

I

I

D

E

E

A

V

T

V

W

K

E

A

A

F

L

K

K

G

Y

I

A

T

K

N

R

V

P

G

R

I

V

H

M

I

L

F

F

I

T

A

S

T

T

S

S

P

E

I

I

H

H

R

M

E

K

R

Y

K

K

L

L

G

K

M

K

T

T

K

K

Q

E

E

E

V

V

V

I

D

E

K

M

A

A

V

V

E

N

S

S

V

V

A

K

Y

Y

A

A

S

K

Q

S

F

L

-

G

F

F

D

K

H

D

I

I

E

Q

F

F

S

G

C

C

E

E

D

D

S

G

T

G

R

R

T

T

E

E

L

K

E

D

F

F

L

I

R

C

E

K

I

I

S

L

E

G

K

E

V

S

I

I

E

K

A

A

G

G

A

A

T

T

V

T

L

V

N

G

F

F

P

A

D

D

T

T

V

V

G
|
G

Y

I

T

N

T

M

P

P

E

Q

E

E

Y

F

T

G

E

E

L

L

F

V

Q

A

Y

Y

I

V

L

I

N

E

H

N

T

T

R

P

G

G

I

A

E

D

K

D

V

I

I

V

L

F

S

A

C

I

H

H

C

C

H

H

D

N

D

D

L

L

G

G

L

V

A

A

L

T

S

A

N

N

S

T

I

I

A

S

A

G

M

I

K

C

A

A

G

G

A

A

L

R

Q

Q

I

V

E

E

G

V

C

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

V

A

A

P

L

L

E

E

V

V

A

V

A

M

L

A

I

L

E

K

T

C

R

R

S

G

E

E

H

S

Y

L

Q

M

R

D

K

G

-

V

-

Y

T

T

G

K

I

I

N

D

L

S

K

R

E

Q

I

I

A

M

K

A

T

T

S

S

N

K

L

M

V

V

S

Q

R

E

L

H

T

T

G

G

I

M

S

Y

I

V

P

Q

P

P

N

H

K

K

A

P

V

I

V

V

G

G

G

D

N

N

A

C

Y

F

A

V

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

I

I

L

K

K

D

N

K

R

S

S

T

T

Y

Y

E

E

I

I

L

L

K

S

P

P

E

E

S

D

V

V

G

G

F

I

S

V

E

K

T

S

K

E

-

N

-

S

-

G

L

I

V

V

L

L

G

G

K

K

L

L

S

S

G

G

R

R

H

H

A

A

L

V

K

K

K

D

R

R

Y

L

E

K

E

E

L

L

G

G

Y

Y

H

E

V

I

D

S

D

D

Q

E

Q

K

L

F

K

N

E

D

I

I

F

F

T

S

K

R

Y

Y

K

R

E

E

L

L

T

T

D

K

R

D

K

K

E

R

I

I

F

T

D

D

N

A

D

D

I

L

I

K

A

A

L

L

L

V

G263 (= G174) mutation to E: In gsm2-1; loss of activity and lack of C6, C7 and C8 aliphatic glucosinolates.

6e1jA Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
44% identity, 73% coverage: 3:380/520 of query aligns to 15:407/409 of 6e1jA

query
sites
6e1jA

K

K

D

N

Q

Y

V

V

I

R

L

V

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q
|
Q

T

S

P

P

G

G

V

A

N

A

L

L

N

T

E

P

N

P

D

Q

K

K

V

I

K

E

I

I

A

A

L

R

R

Q

L

L

E

A

K

K

L

L

G

R

I

V

D

D

I

I

I

M

E

E

A

V

G

G

F
|
F

A

P

A

V

A
x
S

S

S

P

E

G

E

D

E

A

F

K

E

A

T

V

I

R

Q

A

T

V

I

A

A

E

K

A

T

V

V

-

G

-

N

-

E

-
x
V

-

D

-
x
E

-

E

-

T

-

G

H

Y

K

I

P

P

L

V

V

I

V

C

S

V

L
x
I

A

A

R
|
R

T

S

N

K

K

E

N

R

D

D

I

I

D

K

E

A

V

A

V

W

K

E

A

S

F

V

K

K

G

Y

I

A

T

K

N

R

V

P

G

R

I

I

H

V

I

I

F
|
F

I

T

A

S

T

T

S

S

P

D

I

I

H

H

R

L

E

K

R

Y

K
|
K

L
|
L

G

K

M

M

T

T

K

R

Q

E

E

E

V

V

D

D

K

M

A

V

V

A

E

S

S

S

V

I

A

R

Y

F

A

A

S

K

Q

S

F

L

-

G

F

F

D

E

H

D

I

I

E

E

F

F

S

G

C

C

E

E

D

D

S

G

T

G

R

R

T

S

E

D

L

K

E

D

F

Y

L

I

R

C

E

T

I

V

S

F

E

E

K

E

V

A

I

I

E

K

A

A

G

G

A

A

T

T

V

T

L

L

N

A

F

C

P
|
P

D

D

T
|
T

V

V

G

G

Y

I

T

N

T

M

P

P

E

H

E

E

Y

Y

T

G

E

K

L

L

F

V

Q

R

Y

Y

I

I

L

K

N

A

H

N

T

T

R

P

G

G

I

I

E

D

K

D

V

V

I

I

L

F

S

S

C

A

H
|
H

C

C

H
|
H

D

N

D

D

L

L

G

G

L

V

A

A

L

T

S

A

N

N

S

T

I

I

A

A

A

G

M

I

K

C

A

A

G

G

A

A

L

R

Q

Q

I

V

E

E

G

V

C

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

V

A

A

P

L

L

E

E

V

V

A

V

A

M

L

A

I

L

E

K

T

C

R

R

S

G

E

A

H

F

Y

V

Q

M

R

G

K

G

-

V

-

Y

T

T

G

R

I

I

N

D

L

T

K

R

E

Q

I

I

A

M

K

A

T

T

S

S

N

K

L

M

V

V

S

Q

R

E

L

Y

T

T

G

G

I

L

S

Y

I

V

P

Q

P

P

N

H

K

K

A

P

V

I

V

V

G

G

G

A

N

N

A

C

Y

F

A

V

H

H

S

E

S
|
S

G
|
G

I
|
I

H

H

Q

Q

D
|
D

G

G

V

I

I

L

K
|
K

D

N

K

R

S

S

T

T

Y

Y

E

E

I

I

L

I

K

S

P

P

E

E

S

D

V

V

G

G

F

V

S

V

E

K

T

S

K

Q

-

N

-

S

-

G

L

I

V

V

L

L

G

G

K

K

L
|
L

S

S

G

G

R
|
R

H
|
H

A

A

L

V

K

K

K

G

R

R

Y

L

E

K

E

E

L

L

G

G

Y

Y

H

E

V

I

D

S

D

D

Q

E

Q

K

L

L

K

N

E

E

I

V

F

F

T

S

K

R

Y

F

K

R

E

D

L

L

T

T

D

K

R

Q

K

K

E

R

I

V

F

T

D

D

N

D

D

D

I

L

I

K

A

A

L

L

L

V

binding coenzyme a: Q30 (= Q18), F60 (= F48), S63 (≠ A51), I95 (≠ L74), R97 (= R76), F121 (= F100), K132 (= K111), L133 (= L112), S322 (= S298), G323 (= G299), I324 (= I300), D327 (= D303), K331 (= K307), L359 (= L332), R362 (= R335), H363 (= H336)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: P192 (= P170), T194 (= T172), H225 (= H203), H227 (= H205)
binding manganese (ii) ion: D27 (= D15), V82 (vs. gap), E84 (vs. gap), H225 (= H203), H227 (= H205)

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 73% coverage: 6:382/520 of query aligns to 85:476/506 of Q9FG67

query
sites
Q9FG67

V

V

I

R

L

V

F

F

D

D

T

T

L

L

R

R

D

D

G

G

E

E

Q

Q

T

S

P

P

G

G

V

G

N
x
S

L

L

N

T

E

P

N

P

D

Q

K

K

V

L

K

E

I

I

A

A

L

R

R

Q

L

L

E

A

K

K

L

L

G

R

I

V

D

D

I

I

I

M

E

E

A

V

G

G

F

F

A

P

A

G

A

S

S

S

P

E

G

E

D

E

A

L

K

E

A

T

V

I

R

K

A

T

V

I

A

A

E

K

A

T

V

V

-

G

-

N

-

E

-

V

-

D

-

E

-

T

-

G

H

Y

K

V

P

P

L

V

V

I

V

C

S

A

L

I

A

A

R

R

T

C

N

K

K

H

N

R

D

D

I

I

D

E

E

A

V

T

V

W

K

E

A

A

F

L

K

K

G

Y

I

A

T

K

N

R

V

P

G

R

I

I

H

L

I

V

F

F

I

T

A

S

T

T

S

S

P

D

I

I

H

H

R

M

E

K

R

Y

K

K

L

L

G

K

M

K

T

T

K

Q

Q

E

E

E

V

V

V

I

D

E

K

M

A

A

V

V

E

S

S

S

V

I

A

R

Y

F

A

A

S

K

Q

S

F

L

-

G

F

F

D

N

H

D

I

I

E

Q

F

F

S

G

C

C

E

E

D

D

S

G

T

G

R

R

T

S

E

D

L

K

E

D

F

F

L

L

R

C

E

K

I

I

S

L

E

G

K

E

V

A

I

I

E

K

A

A

G

G

A

V

T

T

V

V

L

V

N

T

F

I

P

G

D

D

T

T

V

V

G

G

Y

I

T

N

T

M

P

P

E

H

E

E

Y

Y

T

G

E

E

L

L

F

V

Q

T

Y

Y

I

L

L

K

N

A

H

N

T

T

R

P

G

G

I

I

E

D

K

D

V

V

I

V

L

V

S
x
A

C

V

H

H

C

C

H

H

D

N

D

D

L

L

G

G

L

L

A

A

L

T

S

A

N

N

S

S

I

I

A

A

A

G

M

I

K

R

A

A

G

G

A

A

L

R

Q

Q

I

V

E

E

G

V

C

T

I

I

N

N

G

G

I

I

G

G

E

E

R

R

A

S

G

G

N

N

V

A

A

S

L

L

E

E

V

V

A

V

A

M

L

A

I

L

E

K

T

C

R

R

S

G

E

A

H

Y

Y

V

Q

I

R

N

K

G

-

V

-

Y

T

T

G

K

I

I

N

D

L

T

K

R

E

Q

I

I

A

M

K

A

T

T

S

S

N

K

L

M

V

V

S

Q

R

E

L

Y

T

T

G

G

I

L

S

Y

I

V

P

Q

P

A

N

H

K

K

A

P

V

I

V

V

G

G

G

A

N

N

A

C

Y

F

A

V

H

H

S

E

S

S

G

G

I

I

H

H

Q

Q

D

D

G

G

V

I

I

L

K

K

D

N

K

R

S

S

T

T

Y

Y

E

E

I

I

L

L

K

S

P

P

E

E

S

D

V

I

G

G

F

I

S

V

E

K

T

S

K

Q

-

N

-

S

-

G

L

L

V

V

L

L

G

G

K

K

L

L

S

S

G

G

R

R

H

H

A

A

L

V

K

K

K

D

R

R

Y

L

E

K

E

E

L

L

G

G

Y

Y

H

E

V

L

D

D

Q

E

Q

K

L

L

K

N

E

A

I

V

F

F

T

S

K

L

Y

F

K

R

E

D

L

L

T

T

D

K

R

N

K

K

E

R

I

I

F

T

D

D

N

A

D

D

I

L

I

K

A

A

L

L

L

V

E

T

S

S

S102 (≠ N23) mutation to F: In gsm1-1; loss of conversion of C3 to C4 glucosinolates.
A290 (≠ S201) mutation to T: In gsm1-2; loss of conversion of C3 to C4 glucosinolates.

3rmjB Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
48% identity, 62% coverage: 4:323/520 of query aligns to 2:308/308 of 3rmjB

query
sites
3rmjB

D

N

Q

R

V

V

I

I

L

I

F

F

D

D

T

T

L

L

R

R

D
|
D

G

G

E

E

Q
|
Q

T

S

P

P

G

G

V

A

N

A

L

M

N

T

E

K

N

E

D

E

K

K

V

I

K

R

I

V

A

A

L

R

R

Q

L

L

E

E

K

K

L

L

G

G

I

V

D

D

I

I

E

E

A

A

G

G

F

F

A

A

S

S

P

P

G

G

D

D

A

F

K

E

A

A

V

V

R

N

A

A

V

I

A

A

E

K

A

T

V

I

H

T

K

K

P

S

L

T

V

V

V

C

S

S

L

L

A

S

R

R

T

A

N

I

K

E

N

R

D

D

I

I

D

R

E

Q

V

A

V

G

K

E

A

A

F

V

K

A

G

P

I

A

T

P

N

K

V

K

G

R

I

I

H

H

I

T

F

F

I

I

A

A

T

T

S

S

P

P

I

I

H

H

R

M

E

E

R

Y

K

K

L

L

G

K

M

M

T

K

K

P

Q

K

E

Q

V

V

V

I

D

E

K

A

A

A

V

V

E

K

S

A

V

V

A

K

Y

I

A

A

S

R

Q

E

F

Y

F

T

D

D

H

D

I

V

E

E

F

F

S

S

C

C

E

E

D

D

S

A

T

L

R

R

T

S

E

E

L

I

E

D

F

F

L

L

R

A

E

E

I

I

S

C

E

G

K

A

V

V

I

I

E

E

A

A

G

G

A

A

T

T

V

T

L

I

N

N

F

I

P

P

D

D

T

T

V

V

G

G

Y

Y

T

S

T

I

P

P

E

Y

E

K

Y

T

T

E

E

E

L

F

F

F

Q

R

Y

E

I

L

L

I

N

A

H

K

T

T

R

P

G

N

I

G

E

G

K

K

V

V

I

V

L

W

S

S

C

A

H
|
H

C

C

H
|
H

D

N

D

D

L

L

G

G

L

L

A

A

L

V

S

A

N

N

S

S

I

L

A

A

M

L

K

K

A

G

G

G

A

A

L

R

Q

Q

I

V

E

E

G

C

C

T

I

V

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N
|
N

V

A

A

S

L

V

E

E

V

I

A

V

A

M

L

A

I

L

E

K

T

V

R

R

S

H

E

D

H

L

Y

F

Q

G

R

L

K

E

T

T

G

G

I

I

N

D

L

T

K

T

E

Q

I

I

A

V

K

P

T

S

S

S

N

K

L

L

V

V

S

S

R

T

L

I

T

T

G

G

I

Y

S

P

I

V

P

Q

P

P

N

N

K

K

A

A

V

I

V

V

G

G

G

A

N

N

A

A

Y

F

A

S

H

H

S

-

G

-

I

-

H

-

Q

-

D

-

G

-

V

-

I

-

K

-

D

-

K

-

S

E

T

T

Y

Y

E

E

I

I

L

M

K

S

P

A

E

E

S

S

V

V

G

G

F

W

S

A

active site: Q16 (= Q18)
binding manganese (ii) ion: D13 (= D15), H201 (= H203), H203 (= H205), N237 (= N239)

6ktqA Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
35% identity, 73% coverage: 5:382/520 of query aligns to 21:389/399 of 6ktqA

query
sites
6ktqA

Q

K

V

V

I

G

L

I

F

L

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q

Q

T

T

P

P

G

G

V

V

N

V

L

F

N

T

E

T

N

D

D

Q

K

R

V

V

K

E

I

I

A

A

L

K

R

A

L

L

E

S

K

D

L

I

G

G

I

V

D

Q

I

M

I

I

E

E

A

A

G

G

F

H

A

P

A

A

A
x
V

S

S

P

P

G

D

D

I

A

Y

K

E

A

G

V

I

R

R

A

R

V

I

-

I

-

K

-

L

-

K

A

R

E

E

A

G

V

V

H

I

K

K

P

S

L

E

V

I

V

V

S

A

L

H

A

S

R
|
R

T
x
A

N

V

K

K

N

R

D

D

I

I

D

E

E

V

V

G

V

A

K

E

A

-

F

-

K

-

G

-

I

I

T

E

N

A

V

D

G

R

I

I

H

A

I

I

F
|
F

I

Y

A

G

T

I

S

S

P

D

I

T

H

H

R

L

E

K

R

A

K

K

L
x
H

G

H

M

T

T

T

K

R

Q

D

E

E

V

A

V

L

D

R

K

S

A

I

V

A

E

E

S

T

V

V

A

S

Y

Y

A

A

S

K

Q

S

F

H

F

G

D

V

H

K

I

V

E
x
R

F

F

S
x
T

C

A

E
|
E

D

D

S

A

T

T

R

R

T

A

E

D

L

Y

E

Q

F

Y

L

L

R

L

E

E

I

V

S

I

E

K

K

T

V

V

I

R

E

D

A

A

G

G

A

A

T

D

V

R

L

V

N
x
S

F

I

P

A

D

D

T
|
T

V

V

G

G

Y

V

T

L

T

Y

P

P

E

S

E

R

Y

T

T

R

E

E

L

L

F

F

Q

K

Y

D

I

L

L

T

N

S

H

R

T

F

R

P

G

D

I

I

E

E

K

-

V

-

I

-

L

F

S

D

C

I

H
|
H

C

A

H
|
H

D

N

D

D

L

L

G

G

L

M

A

A

L

V

S

A

N

N

S

V

I

L

A

A

M

A

K

E

A

G

G

G

A

A

L

T

Q

I

I

I

E

H

G

T

C

T

I

L

N

N

G

G

I

L

G

G

E

E

R

R

A

V

G

G

N

I

V

A

A

P

L

L

E

Q

E

V

V

V

A

A

L

A

I

L

E

K

T

Y

R

-

S

-

E

-

H

H

Y

F

Q

G

R

I

K

E

T

V

G

-

I

V

N

D

L

L

K

K

E

K

I

L

A

S

K

E

T

V

S

A

N

S

L

L

V

V

S

E

R

K

L

Y

T

S

G

G

I

I

S

A

I

L

P

P

N

N

K

F

A

P

V

I

V

T

G

G

G

D

N

Y

A

A

Y

F

A

V

H

H

S

K

S

A

G

G

I

V

H

H

Q

V

D

A

G

G

V

V

I

L

K

N

D

D

K

P

S

K

T

T

Y

Y

E

E

I

F

L

L

K

P

P

P

E

E

S

T

V

F

G

G

F

R

S

S

E

R

T

-

K

D

L

Y

V

V

L

I

G

D

K

K

L

Y

S

T

G

G

R

K

H

H

A

A

L

V

K

K

K

D

R

R

Y

F

E

D

E

R

L

L

G

G

Y

V

H

K

V

L

D

T

D

D

Q

S

Q

E

L

I

K

D

E

Q

I

V

F

L

T

A

K

K

Y

I

K

K

E

S

L

-

T

N

D

P

R

N

K

V

K

R

E

F

I

Y

F

R

D

D

N

V

D

D

I

L

A

E

L

L

L

A

E

E

S

S

binding 2-oxoglutaric acid: R30 (= R14), R154 (≠ E138), T156 (≠ S140), E158 (= E142), S184 (≠ N168), T188 (= T172), H216 (= H203), H218 (= H205)
binding coenzyme a: V67 (≠ A51), R96 (= R76), A97 (≠ T77), F116 (= F100), H128 (≠ L112), E158 (= E142)
binding zinc ion: E31 (≠ D15), H216 (= H203), H218 (= H205)

3ivtB Homocitrate synthase lys4 bound to 2-og (see paper)
31% identity, 71% coverage: 8:374/520 of query aligns to 32:389/400 of 3ivtB

query
sites
3ivtB

L

I

F

I

D

E

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

T

F

P

A

G

N

V

A

N

F

L

F

N

D

E

T

N

E

D

K

K

K

V

I

K

Q

I

I

A

A

L

K

R

A

L

L

E

D

K

N

L

F

G

G

I

V

D

D

I

Y

I

I

E

E

A

L

G

T

F

S

A

P

A

V

A

A

S

S

P

E

G

Q

D

S

A

R

K

Q

A

D

V

C

R

E

A

A

V

I

A

C

E

K

A

L

V

G

H

L

K

K

P

C

L

K

V

I

V

L

S

T

L
x
H

A

I

R

R

T

C

N

H

K

M

N

D

D

D

I

A

D

R

E

V

V

A

V

V

K

E

A

T

F

-

K

-

G

G

I

V

T

D

N

-

V

-

G

G

I

V

H

D

I

V

F

V

I

I

A

G

T

T

S

S

P

Q

I

Y

H

L

R

R

E

K

R

Y

K

S

L

H

G

G

M

K

T

D

K

M

Q

T

E

Y

V

I

D

D

K

S

A

A

V

T

E

E

S

V

V

I

A

N

Y

F

A

V

S

K

Q

S

F

K

F

G

D

I

H

E

I

V

E
x
R

F

F

S
|
S

C

S

E

E

D

D

S

S

T

F

R

R

T

S

E

D

L

L

E

V

F

D

L

L

R

L

E

S

I

L

S

Y

E

K

K

A

V

V

I

D

E

K

A

I

G

G

A

V

T

N

V

R

L

V

N

G

F

I

P

A

D

D

T
|
T

V

V

G

G

Y

C

T

A

T

T

P

P

E

R

E

Q

Y

V

T

Y

E

D

L

L

F

I

Q

R

Y

T

I

L

L

-

N

-

H

-

T

-

R

R

G

G

I

V

E

V

K

S

V

C

I

D

L

I

S

E

C

C

H
|
H

C

F

H
|
H

D

N

D

D

L

T

G

G

L

M

A

A

L

I

S

A

N

N

S

A

I

Y

A

C

A

A

M

L

K

E

A

A

G

G

A

A

L

T

Q

H

I

I

E

D

G

T

C

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

V

T

A

P

L

L

E

G

E

A

V

L

A

L

A

A

-

R

L

M

I

Y

E

V

T

T

R

D

S

R

E

E

H

Y

Y

I

Q

T

R

H

K

K

T

Y

G

K

I

L

N

N

L

-

K

-

E

Q

I

L

A

R

K

E

T

L

S

E

N

N

L

L

V

V

S

A

R

D

L

A

T

V

G

E

I

V

S

Q

I

I

P

P

P

F

N

N

K

N

A

Y

V

I

V

T

G

G

G

M

N

C

A

A

Y

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

I

L

K

A

D

N

K

P

S

S

T

T

Y

Y

E

E

I

I

L

L

K

K

P

P

E

E

S

D

V

F

G

G

F

M

S

S

E

R

T

Y

K

V

L

H

V

V

L

G

G

S

K

R

L

L

S

T

G

G

R

W

H

N

A

A

L

I

K

K

K

S

R

R

Y

A

E

E

E

Q

L

L

G

N

Y

L

H

H

V

L

D

T

D

D

Q

A

Q

Q

L

A

K

K

E

E

I

L

F

T

T

V

K

R

Y

I

K

K

E

K

L

L

T

A

D

D

R

V

K

R

K

T

E

L

I

A

F

M

D

D

N

D

active site: Q42 (= Q18)
binding 2-oxoglutaric acid: R38 (= R14), H98 (≠ L74), R158 (≠ E138), S160 (= S140), T192 (= T172), H219 (= H203), H221 (= H205)
binding zinc ion: E39 (≠ D15), H219 (= H203), H221 (= H205)

Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
31% identity, 71% coverage: 8:374/520 of query aligns to 37:394/418 of Q9Y823

query
sites
Q9Y823

L

I

F

I

D

E

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

T

F

P

A

G

N

V

A

N

F

L

F

N

D

E

T

N

E

D

K

K

K

V

I

K

Q

I

I

A

A

L

K

R

A

L

L

E

D

K

N

L

F

G

G

I

V

D

D

I

Y

I

I

E
|
E

A

L

G

T

F

S

A

P

A

V

A

A

S

S

P

E

G

Q

D

S

A

R

K

Q

A

D

V

C

R

E

A

A

V

I

A

C

E

K

A

L

V

G

H

L

K

K

P

C

L

K

V

I

V

L

S

T

L
x
H

A

I

R

R

T

C

N

H

K

M

N

D

D

D

I

A

D

R

E

V

V

A

V

V

K

E

A

T

F

-

K

-

G

G

I

V

T

D

N

-

V

-

G

G

I

V

H
x
D

I

V

F

V

I

I

A

G

T

T

S

S

P

Q

I

Y

H

L

R

R

E

K

R

Y

K

S

L

H

G

G

M

K

T

D

K

M

Q

T

E

Y

V

I

D

D

K

S

A

A

V

T

E

E

S

V

V

I

A

N

Y

F

A

V

S

K

Q

S

F

K

F

G

D

I

H

E

I

V

E
x
R

F

F

S
|
S

C

S

E
|
E

D

D

S

S

T

F

R

R

T

S

E

D

L

L

E

V

F

D

L

L

R

L

E

S

I

L

S

Y

E

K

K

A

V

V

I

D

E

K

A

I

G

G

A

V

T

N

V

R

L

V

N

G

F

I

P

A

D

D

T
|
T

V

V

G

G

Y

C

T

A

T

T

P

P

E

R

E

Q

Y

V

T

Y

E

D

L

L

F

I

Q

R

Y

T

I

L

L

-

N

-

H

-

T

-

R

R

G

G

I

V

E

V

K

S

V

C

I

D

L

I

S
x
E

C

C

H
|
H

C

F

H
|
H

D

N

D

D

L

T

G

G

L

M

A

A

L

I

S

A

N

N

S

A

I

Y

A

C

A

A

M

L

K

E

A

A

G

G

A

A

L

T

Q

H

I

I

E

D

G

T

C

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

V

T

A

P

L

L

E

G

E

A

V

L

-

L

A

A

A

R

L

M

I

Y

E

V

T

T

R

D

S

R

E

E

H

Y

Y

I

Q

T

R

H

K

K

T

Y

G

K

I

L

N

N

L

-

K

-

E

Q

I

L

A
x
R

K

E

T

L

S

E

N

N

L

L

V

V

S

A

R

D

L

A

T

V

G

E

I

V

S

Q

I

I

P

P

P

F

N

N

K

N

A

Y

V

I

V

T

G

G

G

M

N

C

A

A

Y

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

I

L

K

A

D

N

K

P

S

S

T

T

Y
|
Y

E

E

I

I

L

L

K

K

P

P

E

E

S

D

V

F

G

G

F

M

S

S

E

R

T

Y

K

V

L

H

V

V

L

G

G

S

K

R

L

L

S

T

G

G

R

W

H

N

A

A

L

I

K

K

K

S

R

R

Y

A

E

E

E
x
Q

L

L

G

N

Y

L

H

H

V

L

D

T

D

D

Q

A

Q

Q

L

A

K

K

E

E

I

L

F

T

T

V

K

R

Y

I

K

K

E

K

L

L

T

A

D

D

R

V

K

R

K

T

E

L

I

A

F

M

D

D

N

D

R43 (= R14) binding 2-oxoglutarate; mutation R->A,K,Q: Abolishes the catalytic activity.
E44 (≠ D15) binding 2-oxoglutarate; binding L-lysine; binding Zn(2+)
Q47 (= Q18) mutation to A: Abolishes the catalytic activity.
E74 (= E45) mutation to A: Abolishes the catalytic activity.; mutation to Q: Results in a moderate decrease in the turnover number and a slight increase in the Km value for each substrate.
H103 (≠ L74) binding 2-oxoglutarate; mutation to A: Substantially impairs catalytic efficiency.
D123 (≠ H98) binding L-lysine; mutation to N: Does not affect the catalytic activity but impairs L-lysine inhibition.
R163 (≠ E138) binding 2-oxoglutarate; mutation R->A,Q: Abolishes the catalytic activity.; mutation to K: Severely diminishes affinity for 2-oxoglutarate and substantially impairs catalytic efficiency.
S165 (= S140) binding 2-oxoglutarate; mutation to A: Results in a moderate decrease in catalytic efficiency.
E167 (= E142) mutation E->A,Q: Abolishes the catalytic activity.
T197 (= T172) binding 2-oxoglutarate; binding L-lysine; mutation to A: Exhibits a 25-fold decrease in catalytic efficiency.; mutation to S: Results in a modest decrease in catalytic efficiency.; mutation to V: Abolishes the catalytic activity.
E222 (≠ S201) mutation to Q: Does not affect the catalytic activity but impairs L-lysine inhibition.
H224 (= H203) binding 2-oxoglutarate; binding Zn(2+)
H226 (= H205) binding 2-oxoglutarate; binding Zn(2+)
R288 (≠ A268) mutation to K: Does not affect the catalytic activity but impairs L-lysine inhibition.
Y332 (= Y312) mutation to A: Abolishes the catalytic activity.; mutation to F: Results in a decrease in catalytic efficiency.
Q364 (≠ E344) mutation to R: Does not affect the catalytic activity but impairs L-lysine inhibition.

3mi3A Homocitrate synthase lys4 bound to lysine (see paper)
31% identity, 71% coverage: 8:374/520 of query aligns to 14:360/370 of 3mi3A

query
sites
3mi3A

L

I

F

I

D

E

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

T

F

P

A

G

N

V

A

N

F

L

F

N

D

E

T

N

E

D

K

K

K

V

I

K

Q

I

I

A

A

L

K

R

A

L

L

E

D

K

N

L

F

G

G

I

V

D

D

I

Y

I

I

E

E

A

L

G

T

F

S

A

P

A

V

A

A

S

S

P

E

G

Q

D

S

A

R

K

Q

A

D

V

C

R

E

A

A

V

I

A

C

E

K

A

L

V

G

H

L

K

K

P

C

L

K

V

I

V

L

S

T

L

H

A

I

R

R

T

C

N

H

K

M

N

D

D

D

I

A

D

R

E

V

V

A

V

V

K

E

A

T

F

-

K

-

G

G

I

V

T

D

N

-

V

-

G

G

I

V

H
x
D

I

V

F

V

I

I

A

G

T

T

S

S

P

M

I

T

H

Y

R

-

E

-

R

-

K

-

L

-

G

-

M

-

T

-

K

-

Q

-

E

-

V

I

D

D

K

S

A

A

V

T

E

E

S

V

V

I

A

N

Y

F

A

V

S

K

Q

S

F

K

F

G

D

I

H

E

I

V

E

R

F

F

S

S

C

S

E

E

D

D

S

S

T

F

R

R

T

S

E

D

L

L

E

V

F

D

L

L

R

L

E

S

I

L

S

Y

E

K

K

A

V

V

I

D

E

K

A

I

G

G

A

V

T

N

V

R

L

V

N

G

F

I

P

A

D

D

T
|
T

V

V

G

G

Y

C

T

A

T

T

P

P

E

R

E

Q

Y

V

T

Y

E

D

L

L

F

I

Q

R

Y

T

I

L

L

-

N

-

H

-

T

-

R

R

G

G

I

V

E

V

K

S

V

C

I

D

L

I

S

E

C

C

H
|
H

C

F

H
|
H

D

N

D

D

L

T

G

G

L

M

A

A

L

I

S

A

N

N

S

A

I

Y

A

C

A

A

M

L

K

E

A

A

G

G

A

A

L

T

Q

H

I

I

E

D

G

T

C

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

V

T

A

P

L

L

E

G

E

A

V

L

A

L

A

A

-

R

L

M

I

Y

E

V

T

T

R

D

S

R

E

E

H

Y

Y

I

Q

T

R

H

K

K

T

Y

G

K

I

L

N

N

L

-

K

-

E

Q

I

L

A

R

K

E

T

L

S

E

N

N

L

L

V

V

S

A

R

D

L

A

T

V

G

E

I

V

S

Q

I

I

P

P

P

F

N

N

K

N

A

Y

V

I

V

T

G

G

G

M

N

C

A

A

Y

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

I

L

K

A

D

N

K

P

S

S

T

T

Y

Y

E

E

I

I

L

L

K

K

P

P

E

E

S

D

V

F

G

G

F

M

S

S

E

R

T

Y

K

V

L

H

V

V

L

G

G

S

K

R

L

L

S

T

G

G

R

W

H

N

A

A

L

I

K

K

K

S

R

R

Y

A

E

E

E

Q

L

L

G

N

Y

L

H

H

V

L

D

T

D

D

Q

A

Q

Q

L

A

K

K

E

E

I

L

F

T

T

V

K

R

Y

I

K

K

E

K

L

L

T

A

D

D

R

V

K

R

K

T

E

L

I

A

F

M

D

D

N

D

active site: Q24 (= Q18)
binding lysine: R20 (= R14), D100 (≠ H98), T163 (= T172), H190 (= H203), H192 (= H205)
binding zinc ion: E21 (≠ D15), H190 (= H203), H192 (= H205)

O87198 Homocitrate synthase; HCS; EC 2.3.3.14 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
32% identity, 71% coverage: 1:367/520 of query aligns to 1:358/376 of O87198

query
sites
O87198

M

M

K

R

K

E

D

W

Q

K

V

I

I

I

L

-

F

-

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q

Q

T

F

P

E

G

K

V

A

N

N

L

F

N

S

E

T

N

Q

D

D

K

K

V

V

K

E

I

I

A

A

L

K

R

A

L

L

E

D

K

E

L

F

G

G

I

I

D

E

I

Y

I

I

E

E

A

V

G

T

F

T

A

P

A

V

A

A

S

S

P

P

G

Q

D

S

A

R

K

K

A

D

V

A

R

E

A

V

V

L

A

A

E

S

A

L

V

G

H

L

K

K

P

A

L

K

V

V

S

T

L
x
H

A

I

R

Q

T

C

N

R

K

L

N

D

D

A

I

A

D

K

E

V

V

A

V

V

K

E

A

T

F

-

K

-

G

G

I

V

T

Q

N

-

V

-

G

G

I

I

H
x
D

I

L

F

L

I

F

A

G

T

T

S

S

P

K

I

Y

H

L

R

R

E

A

R

A

K

H

L

-

G

G

M

R

T

D

K

I

Q

P

E

R

V

I

D

E

K

E

A

A

V

K

E

E

S

V

V

I

A

A

Y

Y

A

I

S

R

Q

E

F

A

D

P

H

H

I

V

E

E

-

V

-
x
R

F

F

S
|
S

C

A

E

E

D

D

S

T

T

F

R

R

T

S

E

E

L

E

E

Q

F

D

L

L

R

L

E

A

I

V

S

Y

E

E

K

A

V

V

I

A

E

P

A

Y

G

V

A

D

T

R

V

V

L

-

N

G

F

L

P

A

D

D

T
|
T

V

V

G

G

Y

V

T

A

T

T

P

P

E

R

E

Q

Y

V

T

Y

E

A

L

L

F

V

Q

R

Y

E

I

V

L

-

N

R

H

R

T

V

R

V

G

G

I

-

E

P

K

R

V

V

I

D

L

I

S

E

C

F

H
|
H

C

G

H
|
H

D

N

D

D

L

T

G

G

L

C

A

A

L

I

S

A

N

N

S

A

I

Y

A

E

A

A

M

I

K

E

A

A

G

G

A

A

L

T

Q

H

I

V

E

D

G

T

C

T

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

V

T

A

P

L

L

E

G

V

F

A

L

A

A

L

R

I

M

E

Y

T

T

R

L

S

Q

E

P

H

E

Y

Y

Q

V

R

R

K

R

T

K

G

-

I

Y

N

K

L

L

K

E

E

M

I

L

A

P

K

E

T

L

S

D

N

R

L

M

V

V

S

A

R

R

L

M

T

V

G

G

I

V

S

E

I

I

P

P

P

F

N

N

K

N

A

Y

V

I

V

T

G

G

G

E

N

T

A

A

Y

F

A

S

H

H

S

K

S

A

G

G

I

M

H

H

Q

L

D

K

G

A

V

I

I

Y

K

I

D

N

K

P

S

E

T

A

Y

Y

E

E

I

P

L

Y

K

P

P

P

E

E

S

V

V

F

G

G

F

V

S

K

E

R

T

K

K

L

L

I

V

I

L

A

G

S

K

R

L

L

S

T

G

G

R

R

H

H

A

A

L

I

K

K

K

A

R

R

Y

A

E

E

L

L

G

G

Y

L

H

H

V

Y

D

G

D

E

Q

E

L

L

K

H

E

R

I

V

F

T

T

Q

K

H

Y

I

K

K

E

A

L

L

T

A

D

D

R

R

R12 (= R14) binding 2-oxoglutarate
E13 (≠ D15) binding Mg(2+)
H72 (≠ L74) binding 2-oxoglutarate; mutation to L: Significant decrease in sensitivity to lysine inhibition. Large decrease in affinity for 2-oxoglutarate. Almost no effect on affinity for acetyl-CoA and on turnover number.
D92 (≠ H98) binding L-lysine
R133 (vs. gap) binding 2-oxoglutarate
S135 (= S140) binding L-lysine
T166 (= T172) binding 2-oxoglutarate; binding L-lysine
H195 (= H203) binding Mg(2+)
H197 (= H205) binding Mg(2+)

3ivsA Homocitrate synthase lys4 (see paper)
31% identity, 71% coverage: 8:375/520 of query aligns to 14:356/364 of 3ivsA

query
sites
3ivsA

L

I

F

I

D

E

T

S

T

T

L

L

R

R

D
x
E

G

G

E

E

Q
|
Q

T

F

P

A

G

N

V

A

N

F

L

F

N

D

E

T

N

E

D

K

K

K

V

I

K

Q

I

I

A

A

L

K

R

A

L

L

E

D

K

N

L

F

G

G

I

V

D

D

I

Y

I

I

E

E

A

L

G

T

F

S

A

P

A

V

A

A

S

S

P

E

G

Q

D

S

A

R

K

Q

A

D

V

C

R

E

A

A

V

I

A

C

E

K

A

L

V

G

H

L

K

K

P

C

L

K

V

I

V

L

S

T

L

H

A

I

R

R

T

C

N

H

K

M

N

D

D

D

I

A

D

R

E

V

V

A

V

V

K

E

A

T

F

-

K

-

G

G

I

V

T

D

N

-

V

-

G

G

I

V

H

D

I

V

F

V

I

I

A

G

T

T

S

T

P

Y

I

I

H

-

R

-

E

-

R

-

K

-

L

-

G

-

M

-

T

-

K

-

Q

-

E

-

V

-

V

I

D

D

K

S

A

A

V

T

E

E

S

V

V

I

A

N

Y

F

A

V

S

K

Q

S

F

K

F

G

D

I

H

E

I

V

E

R

F

F

S

S

C

S

E

E

D

D

S

S

T

F

R

R

T

S

E

D

L

L

E

V

F

D

L

L

R

L

E

S

I

L

S

Y

E

K

K

A

V

V

I

D

E

K

A

I

G

G

A

V

T

N

V

R

L

V

N

G

F

I

P

A

D

D

T

T

V

V

G

G

Y

C

T

A

T

T

P

P

E

R

E

Q

Y

V

T

Y

E

D

L

L

F

I

Q

R

Y

T

I

L

L

-

N

-

H

-

T

-

R

R

G

G

I

V

E

V

K

S

V

C

I

D

L

I

S

E

C

C

H
|
H

C

F

H
|
H

D

N

D

D

L

T

G

G

L

M

A

A

L

I

S

A

N

N

S

A

I

Y

A

C

A

A

M

L

K

E

A

A

G

G

A

A

L

T

Q

H

I

I

E

D

G

T

C

S

I

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

V

T

A

P

L

L

E

G

E

A

V

L

A

L

A

A

-

R

L

M

I

Y

E

V

T

T

R

D

S

R

E

E

H

Y

Y

I

Q

T

R

H

K

K

T

Y

G

K

I

L

N

N

L

-

K

-

E

Q

I

L

A

R

K

E

T

L

S

E

N

N

L

L

V

V

S

A

R

D

L

A

T

V

G

E

I

V

S

Q

I

I

P

P

P

F

N

N

K

N

A

Y

V

I

V

T

G

G

G

M

N

C

A

A

Y

F

A

T

H

H

S

K

S

A

G

G

I

I

H

H

Q

A

D

K

G

A

V

I

I

L

K

A

D

N

K

P

S

S

T

T

Y

Y

E

E

I

I

L

L

K

K

P

P

E

E

S

D

V

F

G

G

F

M

S

S

E

R

T

Y

K

V

L

H

V

V

L

G

G

S

K

R

L

L

S

T

G

G

R

W

H

N

A

A

L

I

K

K

K

S

R

R

Y

A

E

E

E

Q

L

L

G

N

Y

L

H

H

V

L

D

-

Q

-

Q

Q

L

A

K

K

E

E

I

L

F

T

T

V

K

R

Y

I

K

K

E

K

L

L

T

A

D

V

R

R

K

T

K

L

E

A

I

M

F

D

D

D

N

V

D

D

active site: Q24 (= Q18)
binding cobalt (ii) ion: E21 (≠ D15), H188 (= H203), H190 (= H205)

Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
27% identity, 95% coverage: 8:502/520 of query aligns to 10:511/516 of Q8F3Q1

query
sites
Q8F3Q1

L

I

F

L

D

D

T

V

T

T

L

L

R
|
R

D
|
D

G

G

E

E

Q

Q

T

T

P

R

G

G

V

V

N

S

L

F

N

S

E

T

N

S

D

E

K

K

V

L

K

N

I

I

A

A

-

K

L

F

R

L

L

L

E

Q

K

K

L

L

G

N

I

V

D

D

I

R

I

V

E

E

A

I

G

A

F

S

A

A

A

R

A

V

S

S

P

K

G

G

D

E

A

L

K

E

A

T

V

V

R

Q

A

K

V

I

A

M

E

E

-

W

A

A

V

A

H

T

K

E

P

Q

L

L

V

T

V

E

S

R

L

I

A

E

R

I

T
x
L

N

G

K
x
F

N

V

D

D

I

G

D

N

E

K

V

T

V

V

K

D

A

W

F

I

K

K

G

D

I

S

T

G

N

A

V

K

G

V

I

L

H

N

I

L

F
x
L

I

T

A

K

T

G

S

S

P

L

I

H

H

H

R

L

E

E

R

K

K

Q

L

L

G

G

M

K

T

T

K

P

Q

K

E

E

V

F

D

T

K

D

A

V

V

S

E

F

S

V

V

I

A

E

Y

Y

A

A

S

I

Q

K

F

S

F

G

D

L

H

K

I

I

E

N

F

V

S
x
Y

C

L

E
|
E

D

D

S

W

T

S

-

N

-

G

-

F

R

R

T

N