SitesBLAST
Comparing WP_078429041.1 NCBI__GCF_002019605.1:WP_078429041.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
35% identity, 98% coverage: 4:253/256 of query aligns to 4:254/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
34% identity, 97% coverage: 4:252/256 of query aligns to 4:253/253 of 1g9xB
4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
32% identity, 95% coverage: 1:242/256 of query aligns to 1:225/285 of 4yerA
- binding adenosine-5'-diphosphate: F14 (= F14), F17 (≠ V17), N39 (= N39), G40 (= G40), G42 (= G42), K43 (= K43), T44 (= T44), T45 (≠ S45), T135 (= T152), F136 (≠ L153), S137 (= S154)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 95% coverage: 3:244/256 of query aligns to 1:224/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 95% coverage: 3:244/256 of query aligns to 2:225/371 of P68187
- A85 (= A89) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ Q123) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V133) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V136) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ D138) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ Q143) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G156) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D177) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 95% coverage: 3:244/256 of query aligns to 1:224/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F14), S37 (≠ N39), G38 (= G40), C39 (≠ A41), G40 (= G42), K41 (= K43), S42 (≠ T44), T43 (≠ S45), Q81 (= Q86), R128 (≠ T148), A132 (≠ T152), S134 (= S154), G136 (= G156), Q137 (≠ L157), E158 (= E178), H191 (= H211)
- binding magnesium ion: S42 (≠ T44), Q81 (= Q86)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 95% coverage: 3:244/256 of query aligns to 1:224/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F14), G38 (= G40), C39 (≠ A41), G40 (= G42), K41 (= K43), S42 (≠ T44), T43 (≠ S45), R128 (≠ T148), S134 (= S154), Q137 (≠ L157)
- binding beryllium trifluoride ion: S37 (≠ N39), G38 (= G40), K41 (= K43), Q81 (= Q86), S134 (= S154), G136 (= G156), H191 (= H211)
- binding magnesium ion: S42 (≠ T44), Q81 (= Q86)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 95% coverage: 3:244/256 of query aligns to 1:224/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F14), V17 (≠ A19), G38 (= G40), C39 (≠ A41), G40 (= G42), K41 (= K43), S42 (≠ T44), T43 (≠ S45), R128 (≠ T148), A132 (≠ T152), S134 (= S154), Q137 (≠ L157)
- binding tetrafluoroaluminate ion: S37 (≠ N39), G38 (= G40), K41 (= K43), Q81 (= Q86), S134 (= S154), G135 (≠ Y155), G136 (= G156), E158 (= E178), H191 (= H211)
- binding magnesium ion: S42 (≠ T44), Q81 (= Q86)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
31% identity, 95% coverage: 3:244/256 of query aligns to 1:224/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F14), V17 (≠ A19), G38 (= G40), C39 (≠ A41), G40 (= G42), K41 (= K43), S42 (≠ T44), T43 (≠ S45), R128 (≠ T148), A132 (≠ T152), S134 (= S154), Q137 (≠ L157)
- binding magnesium ion: S42 (≠ T44), Q81 (= Q86)
6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
29% identity, 98% coverage: 3:254/256 of query aligns to 1:237/240 of 6mjpA
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 94% coverage: 5:244/256 of query aligns to 1:222/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F14), S35 (≠ N39), G36 (= G40), C37 (≠ A41), G38 (= G42), K39 (= K43), S40 (≠ T44), T41 (≠ S45), R126 (≠ T148), A130 (≠ T152), S132 (= S154), G134 (= G156), Q135 (≠ L157)
3d31A Modbc from methanosarcina acetivorans (see paper)
32% identity, 99% coverage: 4:256/256 of query aligns to 1:233/348 of 3d31A
Sites not aligning to the query:
7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
33% identity, 93% coverage: 3:241/256 of query aligns to 3:229/263 of 7d0aB
7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
33% identity, 93% coverage: 3:241/256 of query aligns to 3:229/263 of 7d08B
6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
33% identity, 93% coverage: 3:241/256 of query aligns to 1:227/253 of 6z5uK
- binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ F14), S37 (≠ N39), G38 (= G40), G40 (= G42), K41 (= K43), T42 (= T44), T43 (≠ S45), Q86 (= Q86), E164 (= E178), H197 (= H211)
- binding magnesium ion: Q86 (= Q86), E164 (= E178)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 98% coverage: 3:252/256 of query aligns to 2:235/369 of P19566
- L86 (= L90) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P179) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ N184) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
29% identity, 94% coverage: 15:255/256 of query aligns to 37:267/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
- binding phosphoaminophosphonic acid-adenylate ester: 12
7aheC Opua inhibited inward facing (see paper)
29% identity, 94% coverage: 15:255/256 of query aligns to 37:267/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
28% identity, 97% coverage: 5:252/256 of query aligns to 7:230/353 of 1vciA
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
30% identity, 95% coverage: 9:252/256 of query aligns to 11:244/375 of 2d62A
Query Sequence
>WP_078429041.1 NCBI__GCF_002019605.1:WP_078429041.1
MGAILEVENVSLQFGGVKALNDVSYHIKEGEIFSLIGPNGAGKTSMLNCISGLYRPSYGS
IRFKGKEILNMKPYKRTTLGIARAFQNIALFAHMSVLDNLKLGRHTLMKSGLLKGGLYWG
SAQKEEVEHRRAVEEVIDFLQLQDYRHTPVGTLSYGLQKRVEVGRALALEPELILLDEPM
AGMNSSEKEDMSRFILDMHELKNMTVVLIEHDLGVVMDLSDSIAVLDFGKQIGFGTPAEI
QNNPDVIKAYIGEEAI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory