SitesBLAST
Comparing WP_078429421.1 NCBI__GCF_002019605.1:WP_078429421.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
46% identity, 80% coverage: 1:282/351 of query aligns to 4:295/375 of 2d62A
1g291 Malk (see paper)
47% identity, 72% coverage: 1:251/351 of query aligns to 1:255/372 of 1g291
- binding magnesium ion: D69 (= D71), E71 (≠ R73), K72 (≠ R74), K79 (≠ E79), D80 (≠ K80)
- binding pyrophosphate 2-: S38 (= S40), G39 (= G41), C40 (= C42), G41 (= G43), K42 (= K44), T43 (= T45), T44 (= T46)
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
39% identity, 97% coverage: 1:342/351 of query aligns to 4:340/353 of 1vciA
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 80% coverage: 1:282/351 of query aligns to 1:285/393 of P9WQI3
- H193 (= H196) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
8hplC Lpqy-sugabc in state 1 (see paper)
44% identity, 79% coverage: 4:282/351 of query aligns to 3:282/384 of 8hplC
8hprC Lpqy-sugabc in state 4 (see paper)
44% identity, 79% coverage: 4:282/351 of query aligns to 3:284/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (= Y13), S38 (= S40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (≠ T45), Q82 (= Q88), Q133 (≠ E137), G136 (= G140), G137 (= G141), Q138 (= Q142), H192 (= H196)
- binding magnesium ion: S43 (≠ T45), Q82 (= Q88)
8hprD Lpqy-sugabc in state 4 (see paper)
44% identity, 79% coverage: 4:282/351 of query aligns to 3:284/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (= Y13), S38 (= S40), C40 (= C42), G41 (= G43), K42 (= K44), S43 (≠ T45), T44 (= T46), Q82 (= Q88), R129 (= R133), Q133 (≠ E137), S135 (= S139), G136 (= G140), G137 (= G141), Q159 (≠ E163), H192 (= H196)
- binding magnesium ion: S43 (≠ T45), Q82 (= Q88)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 89% coverage: 1:314/351 of query aligns to 1:322/371 of P68187
- A85 (= A91) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ R112) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A118) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V121) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E123) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ G128) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G141) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D162) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ C232) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ L243) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ C263) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (≠ I272) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ M276) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ S278) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G294) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ T300) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (= S314) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 89% coverage: 1:312/351 of query aligns to 1:318/369 of P19566
- L86 (= L92) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P164) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D169) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ T300) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 3:321/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 3:321/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), S37 (= S40), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), Q81 (= Q88), R128 (= R133), A132 (≠ E137), S134 (= S139), G136 (= G141), Q137 (= Q142), E158 (= E163), H191 (= H196)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q88)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 3:321/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R133), S134 (= S139), Q137 (= Q142)
- binding beryllium trifluoride ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q88), S134 (= S139), G136 (= G141), H191 (= H196)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q88)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 3:321/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ T18), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R133), A132 (≠ E137), S134 (= S139), Q137 (= Q142)
- binding tetrafluoroaluminate ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q88), S134 (= S139), G135 (= G140), G136 (= G141), E158 (= E163), H191 (= H196)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q88)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 3:321/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ T18), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R133), A132 (≠ E137), S134 (= S139), Q137 (= Q142)
- binding magnesium ion: S42 (≠ T45), Q81 (= Q88)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
38% identity, 89% coverage: 4:314/351 of query aligns to 1:319/367 of 1q12A