SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078430671.1 NCBI__GCF_002019605.1:WP_078430671.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9LVW7 Carbamoyl phosphate synthase small chain, chloroplastic; Carbamoyl phosphate synthetase glutamine chain; Protein VENOSA 6; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 98% coverage: 4:358/362 of query aligns to 59:429/430 of Q9LVW7

query
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Q9LVW7

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H410 (≠ E339) mutation to Y: In ven6-1; reticulate leaf phenotype.

P0A6F1 Carbamoyl phosphate synthase small chain; Carbamoyl phosphate synthetase glutamine chain; EC 6.3.5.5 from Escherichia coli (strain K12) (see paper)
45% identity, 98% coverage: 5:358/362 of query aligns to 7:380/382 of P0A6F1

query
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P0A6F1

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S47 (= S45) binding L-glutamine
G241 (= G219) binding L-glutamine
G243 (= G221) binding L-glutamine
L270 (= L247) binding L-glutamine
Q273 (= Q250) binding L-glutamine
N311 (= N288) binding L-glutamine
G313 (= G290) binding L-glutamine
F314 (≠ Y291) binding L-glutamine

1ce8B Carbamoyl phosphate synthetase from escherichis coli with complexed with the allosteric ligand imp (see paper)
45% identity, 98% coverage: 5:358/362 of query aligns to 6:379/379 of 1ce8B

query
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1ce8B

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active site: C268 (= C246), H352 (= H331), E354 (= E333)
binding L-ornithine: R119 (= R118), R122 (= R121)

1c3oB Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
45% identity, 98% coverage: 5:358/362 of query aligns to 6:379/379 of 1c3oB

query
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1c3oB

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G

-

L

-

N

-

G

-

M

D

D

Q

L

V

A

K

K

R

E

V

V

S

T

T

T

K

A

D

E

P

A

Y

Y

H

S

C

W

P

T

G

Q

R

G

G

S

-

W

-

T

-

L

-

T

-

G

-

L

-

P

-

E

-

A

-

K

-

E

-

D

-

E

-

L

-

P

H

F

R

H

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V

L

A

V

Y

D

D

F

F

G

G

A

A

K
|
K

H

R

G

N

I

I

I

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R

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C

C

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A

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T

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S

A

A

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E

E

D

I

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L

L

R

K

L

M

Y

N

P

P

D

D

G

G

I

I

M

F

L

L

S

S

N
|
N

G
|
G

P

P

G
|
G

N

D

P

P

V

A

D

P

V

C

P

D

E

Y

G

A

I

I

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M

A

I

I

K

Q

G

K

L

F

L

L

-

E

G

T

K

D

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P

I

V

F

F

G

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I

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C
x
S

L
|
L

G

G

H

H

Q

Q

L

L

A

A

L

L

A

A

C

S

G

G

A

A

K

K

S

T

A

V

K

K

L

M

T

K

F

F

G

G

H

H

R

H

G

G

S

G

N

N

H

H

P

P

V

V

R

K

E

D

L

V

E

E

T

K

G

N

K

V

I

V

D

M

I

I

T

T

S

A

Q

Q

N
|
N

H
|
H

G
|
G

Y
x
F

T

A

I

V

E

D

K

E

D

A

S

T

L

L

E

P

D

-

T

A

R

N

L

L

I

R

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V

T

T

H

H

I

K

A

S

V

L

N

F

D

D

E

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T

T

V

L

E

Q

G

G

V

I

A

H

H

R

L

T

D

D

Y

K

P

P

A

A

F

F

S

S

V

F

Q

Q

Y

G

H
|
H

P

P

E
|
E

A

A

S

S

P

P

G

G

P

P

E

H

D

D

S

A

N

A

H

P

L

L

F

F

T

D

K

H

F

F

I

I

N

E

L

L

I

I

E

E

E

Q

H

Y

K

R

Q

K

S

T

active site: K201 (= K180), S268 (≠ C246), H352 (= H331), E354 (= E333)
binding glutamine: S46 (= S45), N239 (= N218), G240 (= G219), G242 (= G221), S268 (≠ C246), L269 (= L247), N310 (= N288), H311 (= H289), G312 (= G290), F313 (≠ Y291)

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
42% identity, 97% coverage: 5:354/362 of query aligns to 4:359/2225 of P27708

query
sites
P27708

L

L

I

V

L

L

E

E

D

D

G

G

T

S

V

V

F

L

V

R

G

G

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Q

A

P

F

F

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G

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A

E

A

H

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E

S

K

T

E

A

G

G

E

E

V

V

F

F

N

Q

T

T

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G

M
|
M

T

V

G

G

Y

Y

Q

P

E

E

I

A

L

L

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T

D

D

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P

S

S

Y

Y

C

K

A

A

Q

Q

I

I

V

L

T

V

L

L

T

T

Y

Y

P

P

L

L

I

I

G

G

N

N

Y

Y

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G

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I

N

P

R

P

D

D

D

E

-

M

-

D

-

E

-

F

-

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-

L

-

C

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-

W

F

F

E

E

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P

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A

H

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H

A

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A

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L

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G

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A

Q

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H

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L

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Q

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G

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R

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S

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L

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L

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G

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K

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L

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N

E

G

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T

N

E

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E

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L

V

-

K

-

E

-

L

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N

-

A

P

A

F

E

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D

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P

N

N

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A

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-

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-

L

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D

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-

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N

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A

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R
|
R

V

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L

A

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L

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R

A

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-

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-

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A

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A

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P

E

E

A

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A

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N

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E

M33 (= M34) to R: in DEE50; uncertain significance; dbSNP:rs751610198
R177 (= R171) to Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
42% identity, 97% coverage: 5:354/362 of query aligns to 4:359/2225 of P08955

query
sites
P08955

L

L

I

V

L

L

E

E

D

D

G

G

T

S

V

V

F

L

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G

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A

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F

F

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G

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A

E

A

H

V

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S

K

T

E

A

G

G

E

E

V

V

F

F

N

Q

T

T

G

G

M

M

T

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G

G

Y

Y

Q

P

E

E

I

A

L

L

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T

D

D

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S

Y

Y

C

K

A

A

Q

Q

I

I

V

L

T

V

L

L

T

T

Y

Y

P

P

L

L

I

I

G

G

N

N

Y

Y

G

G

I

I

N

P

R

S

D

D

D

E

-

E

-

D

-

E

-

F

-

G

-

L

-

S

-

K

-

W

F

F

E

E

S

S

I

S

E

E

P

N

A

H

I

V

H

A

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G

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L

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V

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G

E

E

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C

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T

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C

A

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I

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D

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Q

A

Q

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E

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L

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Q

M

S

E

G

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N

E

V

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E

S

E

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V

L

V

-

K

-

E

-

L

-

N

-

A

P

A

F

E

V

E

D

P

P

N

N

D

A

Q

R

-

P

-

L

V

A

K

P

R

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S

S

T

I

K

K

D

T

P

P

-

R

Y

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N

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A

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G

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G

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A

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P

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R

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I

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L

A

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L

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A

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C

L

L

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C

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Q

R

L

D

G

C

A

D

E

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V

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V

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P

Y

W

N

N

T

-

S

-

A

-

E

H

E

E

I

L

L

D

R

S

L

Q

Y

K

P

Y

D

D

G

G

I

L

M

F

L

L

S

S

N

N

G

G

P

P

G

G

N

D

P

P

V

A

D

S

V

Y

P

P

E

G

G

V

I

V

E

A

M

T

I

L

K

N

G

R

L

V

L

L

G

S

K

E

V

P

-

N

-

P

-

R

P

P

I

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

L

L

A

A

L

L

A

A

C

I

G

G

A

A

K

K

S

T

A

Y

K

K

L

M

T

R

F

Y

G

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N

R

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H

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N

H

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L

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E

G

T

T

G

G

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R

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C

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F

I

L

T

T

S

S

Q

Q

N

N

H

H

G

G

Y

F

T

A

I

V

E

D

K

A

D

D

S

S

L

L

E

P

D

A

-

G

-

W

T

T

R

P

L

L

I

F

V

T

T

N

H

-

I

-

A

-

V

A

N

N

D

D

E

C

T

S

V

N

E

E

G

G

V

I

A

V

H

H

L

D

D

S

Y

L

P

P

A

F

F

F

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S

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V

Q

Q

Y

F

H

H

P

P

E

E

A

H

S

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P

A

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G

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P

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D

D

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M

N

E

H

L

L

L

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F

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D

K

V

F

F

I

L

N

E

L

T

I

V

E

R

E

E

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P07258 Carbamoyl phosphate synthase arginine-specific small chain; CPS; CPSase; CPSase-arg; Arginine-specific carbamoyl phosphate synthetase, glutamine chain; Carbamoyl phosphate synthase A; CPS-A; Glutamine-dependent carbamoyl phosphate synthetase; EC 6.3.5.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
39% identity, 99% coverage: 2:361/362 of query aligns to 7:380/411 of P07258

query
sites
P07258

K

K

R

A

Q

T

L

F

I

C

L

I

E

Q

D

N

G

G

T

P

V

S

F

F

V

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G

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K

I

A

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F

F

G

G

S

A

E

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H

K

E

S

K

V

E

A

G

G

E

E

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V

F

F

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T

T

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M

L

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

M

S

T

D

D

P

P

S

S

Y

Y

C

R

A

G

Q

Q

I

I

V

L

T

V

L

F

T

T

Y

Q

P

P

L

L

I

I

G

G

N

N

Y

Y

G

G

I

V

-

P

-

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-

G

-

E

N

A

R

R

D

D

D

E

F

Y

E

N

S

L

I

L

E

K

-

Y

-

F

-

E

-

S

P

P

A

H

I

I

H

H

-

V

G

G

L

I

I

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V

V

K

A

E

E

A

Y

A

A

I

Y

Y

Q

P

Y

S

S

N

H

W

W

R

T

C

A

E

V

E

E

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S

I

L

D

A

D

Q

L

W

L

C

K

Q

A

R

K

E

G

G

I

V

P

A

G

A

L

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T

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G

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D

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T

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R

K

E

L

L

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R

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L

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I

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L

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R

L

G

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R

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L

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A

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H

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D

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V

Y

V

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K

N

E

P

L

M

N

K

A

T

A

-

E

-

P

-

N

-

D

N

Q

L

V

V

K

A

R

Q

V

V

S

T

T

T

K

K

D

K

P

P

Y

F

H

H

C

V

-

S

-

A

-

L

P

P

G

G

R

K

G

A

H

K

-

A

R

N

V

V

V

A

L

L

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I

D

D

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C

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G

A

V

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K

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E

G

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N

C

L

L

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Q

K

R

R

D

G

C

A

D

N

V

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V

V

F

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P

Y

Y

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Y

S

R

A

I

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Q

E

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I

V

L

A

R

S

L

E

Y

F

P

-

D

D

G

G

I

I

M

F

L

L

S

S

N

N

G

G

P

P

G

G

N

N

P

P

V

E

D

L

V

C

P

Q

E

A

G

T

I

I

E

S

M

N

I

V

K

R

G

E

L

L

G

N

K

N

-

P

-

V

-

Y

-

D

-

C

V

I

P

P

I

I

F

F

G

G

I

I

C
|
C

L

L

G

G

H

H

Q

Q

L

L

A

A

L

L

A

A

C

S

G

G

A

A

K

S

S

T

A

H

K

K

L

L

T

K

F

Y

G

G

H

N

R

R

G

A

S

H

N

N

H

I

P

P

V

A

R

M

E

D

L

L

E

T

T

T

G

G

K

Q

I

C

D

H

I

I

T

T

S

S

Q

Q

N

N

H
|
H

G

G

Y

Y

T

A

I

V

E

D

K

P

D

E

S

T

L

L

E

P

D

K

T

D

R

Q

L

W

I

K

V

P

T

Y

H

F

I

V

A

N

V

L

N

N

D

D

E

K

T

S

V

N

E

E

G

G

V

M

A

I

H

H

L

L

D

Q

Y

R

P

P

A

I

F

F

S

S

V

T

Q

Q

Y

F

H
|
H

P

P

E

E

A

A

S

K

P

G

G

G

P

P

E

L

D

D

S

T

N

A

H

I

L

L

F

F

T

D

K

K

F

F

I

F

N

D

L

N

I

I

E

E

E

K

H

Y

K

Q

-

L

Q

Q

S

S

R

Q

V

A

R

K

C264 (= C246) mutation to D: Abolishes glutamine-dependent CPSase activity.
H307 (= H289) mutation to D: Abolishes glutamine-dependent CPSase activity.
H349 (= H331) mutation to D: Abolishes glutamine-dependent CPSase activity.

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
39% identity, 98% coverage: 5:357/362 of query aligns to 11:390/2225 of P20054

query
sites
P20054

L

L

I

V

L

L

E

E

D

D

G

G

T

T

V

K

F

L

V

S

G

G

K

Y

A

S

F

F

G

G

S

S

E

E

H

R

E

S

K

V

E

A

G

G

E

E

V

C

V

V

F

F

N

S

T

T

G

G

M

M

T

V

G

G

Y

Y

Q

N

E

E

I

S

L

I

S

S

D

D

P

P

S

S

Y

Y

C

T

A

G

Q

Q

I

I

V

L

T

V

L

F

T

S

Y

F

P

P

L

L

I

I

G

G

N

N

Y

Y

G

G

I

V

-

P

-

S

-

F

-

R

N

E

R

R

D

D

-

P

-

E

-

S

-

G

-

L

-

A

-

V

D

N

F

F

E

E

S

S

I

D

E

K

P

A

A

H

I

V

H

Q

G

A

L

I

I

I

V

C

K

S

E

E

A

Y

A

C

I

D

Y

E

P

Y

S

S

N

H

W

W

R

A

C

A

E

E

E

K

S

S

I

L

D

S

D

E

L

W

L

L

K

K

A

E

K

S

G

N

I

I

P

P

G

G

L

L

E

Y

G

G

I

I

D

D

T

T

R

R

K

A

L

L

T

I

R

T

L

K

I

I

R

R

K

E

H

K

G

G

T

S

L

L

R

K

G

G

R

K

L

V

C

I

-

I

-

G

-

D

-

F

-

D

S

E

M

S

E

K

V

L

N

E

V

F

E

E

E

D

V

I

V

N

K

L

E

R

L

N

N

L

A

V

A

A

E

E

E

V

P

S

N

T

D

K

Q

E

V

I

K

K

R

E

V

Y

S

K

T

A

K

A

D

E

P

N

Y

N

H

K

C

K

P

T

G

G

-

E

-

K

-

R

R

K

G

N

H

K

R

K

V

V

V

I

L

V

V

L

D

D

F

C

G

G

A

I

K

K

H

N

G

N

I

Q

I

I

Q

R

N

C

L

L

I

L

Q

N

R

R

D

G

C

V

D

D

V

L

I

K

V

V

P

P

-

W

-

D

Y

Y

N

D

T

V

S

V

A

A

E

N

E

E

I

S

L

I

R

N

L

D

Y

Y

P

-

D

D

G

G

I

V

M

F

L

I

S

S

N

N

G

G

P

P

G

G

N

D

P

P

V

S

D

L

V

C

P

G

E

K

G

A

I

I

E

E

M

N

I

I

K

R

G

K

L

V

L

L

G

A

-

L

-

P

-

V

K

A

V

K

P

A

I

V

F

F

G

G

I

V

C

C

L

M

G

G

H

N

Q

Q

L

L

A

G

L

L

A

A

C

A

G

G

A

A

K

Q

S

T

A

H

K

K

L

M

T

A

F

F

G

G

H

N

R

R

G

G

S

L

N

N

H

Q

P

P

V

C

R

V

E

D

L

Q

E

I

T

S

G

G

K

R

I

C

D

H

I

I

T

T

S

S

Q

Q

N

N

H

H

G

G

Y

F

T

V

I

I

E

D

K

S

D

N

S

S

L

L

E

P

D

A

T

G

R

S

L

G

I

W

V

K

T

T

H

Y

-

F

I

I

A

N

V

A

N

N

D

D

E

A

T

S

V

N

E

E

G

G

V

I

A

Y

H

H

L

E

D

S

Y

K

P

P

A

W

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

A

S

M

P

A

G

G

P

P

E

T

D

D

S

T

N

E

H

Y

L

L

F

F

T

D

K

N

F

F

I

V

N

D

L

N

I

V

-

C

-

G

-

E

E

Q

H

H

K

K

Q

S

Sites not aligning to the query:

1 modified: N-acetylmethionine

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 98% coverage: 5:360/362 of query aligns to 24:415/2214 of P07259

query
sites
P07259

L

L

I

E

L

L

E

K

D

D

G

G

T

T

V

V

F

L

V

Q

G

G

K

Y

A

S

F

F

G

G

S

A

E

E

H

K

E

S

K

V

E

A

G

G

E

E

V

L

V

V

F

F

N

Q

T

T

G

G

M

M

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

V

S

T

D

D

P

P

S

S

Y

Y

C

E

A

G

Q

Q

I

I

V

L

T

V

L

I

T

T

Y

Y

P

P

L

L

I

V

G

G

N

N

Y

Y

G

G

I

V

N

P

-

D

-

M

-

H

-

L

R

R

D

D

D

E

-

L

-

V

-

E

-

L

-

P

-

R

-

Y

F

F

E

E

S

S

I

N

E

R

P

I

A

H

I

I

H

A

G

G

L

L

I

V

V

I

K

S

E

H

A

Y

A

T

I

D

Y

E

P

Y

S

S

N

H

W

Y

R

L

C

A

E

K

E

S

S

S

I

L

D

G

D

K

L

W

L

L

K

Q

A

N

K

E

G

G

I

I

P

P

G

A

L

V

E

Y

G

G

I

V

D

D

T

T

R

R

K

S

L

L

T

T

R

K

L

H

I

L

R

R

K

D

H

A

G

G

T

S

L

M

R

L

G

G

R

R

L

L

C

S

S

L

M

E

E

K

V

S

N

G

V

S

E

D

E

R

V

T

V

I

K

S

E

R

L

S

N

S

A

S

A

W

E

R

-

S

-

A

-

F

-

D

-

V

-

P

-

E

-

W

-

V

E

D

P

P

N

N

D

V

Q

Q

-

N

-

L

V

V

K

S

R

K

V

V

S

S

T

I

K

N

D

E

P

P

-

K

-

L

Y

Y

H

V

C

P

P

P

-

A

-

D

-

N

-

K

-

H

-

I

-

E

-

L

-

Q

-

T

-

G

-

P

-

D

G

G

R

K

G

V

H

L

R

R

V

I

V

L

L

A

V

I

D

D

F

V

G

G

A

M

K

K

H

Y

G

N

I

Q

I

I

Q

R

N

C

L

F

I

I

Q

K

R

R

D

G

C

V

D

E

V

L

I

K

V

V

P

P

Y

W

N

N

T

Y

S

-

A

-

E

-

E

D

I

F

L

T

R

K

L

E

Y

D

P

Y

D

D

G

G

I

L

M

F

L

I

S

S

N

N

G

G

P

P

G

G

N

D

P

P

V

S

D

V

V

L

P

D

E

D

G

L

I

S

E

Q

M

R

I

L

K

S

G

N

L

V

L

L

-

E

-

A

G

K

K

K

V

T

P

P

I

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

L

L

L

I

A

A

L

R

A

A

C

A

G

G

A

A

K

S

S

T

A

L

K

K

L

L

T

K

F

F

G

G

H

N

R

R

G

G

S

H

N

N

H

I

P

P

V

C

R

T

E

S

L

T

E

I

T

S

G

G

K

R

I

C

D

Y

I

I

T

T

S

S

Q

Q

N

N

H

H

G

G

Y

F

T

A

I

V

E

D

K

V

D

D

S

T

L

L

E

T

D

-

T

S

R

G

L

W

I

K

V

P

T

L

H

F

I

V

A

N

V

A

N

N

D

D

E

D

T

S

V

N

E

E

G

G

V

I

A

Y

H

H

L

S

D

E

Y

L

P

P

A

Y

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

S

S

T

P

P

G

G

P

P

E

R

D

D

S

T

N

E

H

F

L

L

F

F

T

D

K

V

F

F

I

I

N

Q

L

A

I

V

E

K

E

E

H

F

K

K

Q

Y

S

T

R

Q

V

V

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

P05990 Multifunctional protein r; Protein rudimentary; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Drosophila melanogaster (Fruit fly) (see 2 papers)
39% identity, 97% coverage: 5:354/362 of query aligns to 8:376/2224 of P05990

query
sites
P05990

L

L

I

A

L

L

E

E

D

D

G

G

T

T

V

V

F

L

V

P

G

G

K

Y

A

S

F

F

G

G

-

Y

-

V

-

P

S

S

E

E

H

N

E

E

K

S

E

K

-

V

-

G

-

F

-

G

G

G

E

E

V

V

F

F

N

Q

T

T

G

G

M

M

T

V

G

G

Y

Y

Q

T

E

E

I

A

L

L

S

T

D

D

P

R

S

S

Y

Y

C

S

A

G

Q

Q

I

I

V

L

T

V

L

L

T

T

Y

Y

P

P

L

L

I

I

G

G

N

N

Y

Y

G

G

I

V

N

P

R

A

D

P

D

D

-

E

-

D

-

E

-

H

-

G

-

L

-

P

-

L

-

H

F

F

E

E

S

W

I

M

E

K

P

G

A

V

I

V

H

Q

G

A

-

T

-

A

L

L

I

V

V

V

K

G

E

E

A

V

A

A

I

E

Y

E

P

A

S

F

N

H

W

W

R

R

C

K

E

W

E

K

S

T

I

L

D

P

D

D

L

W

L

L

K

K

A

Q

K

H

G

K

I

V

P

P

G

G

L

I

E

Q

G

D

I

I

D

D

T

T

R

R

K

A

L

L

T

T

R

K

L

K

I

L

R

R

K

E

H

Q

G

G

T

S

L

M

R

L

G

G

R

K

L

I

C

V

S

Y

M

E

E

K

V

P

N

P

V

V

E

E

E

G

V

L

V

P

K

K

E

S

L

S

N

F

A

V

A

D

E

P

E

N

P

V

N

R

D

N

Q

L

V

A

K

K

R

E

V

C

S

S

T

V

K

K

D

E

P

R

-

Q

-

V

Y

Y

H

G

C

N

P

P

-

N

G

G

R

K

G

G

H

P

R

R

V

I

V

A

L

I

V

L

D

D

F

C

G

G

A

L

K

K

H

L

G

N

I

Q

I

L

Q

R

N

C

L

L

I

L

Q

Q

R

R

D

G

C

A

D

S

V

V

I

T

V

L

P

P

Y

W

N

S

T

A

S

R

A

L

E

E

E

D

I

-

L

-

R

-

L

E

Y

Q

P

F

D

D

G

A

I

L

M

F

L

L

S

S

N

N

G

G

P

P

G

G

N

N

P

P

V

E

D

S

V

C

P

D

E

Q

G

I

I

V

E

Q

M

Q

I

V

K

R

G

K

L

V

L

I

-

E

G

G

K

Q

V

K

P

P

I

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

L

L

A

A

L

K

A

A

C

I

G

G

A

C

K

S

S

T

A

Y

K

K

L

M

T

K

F

Y

G

G

H

N

R

R

G

G

S

H

N

N

H

L

P

P

V

C

R

L

E

H

L

R

E

A

T

T

G

G

K

R

I

C

D

L

I

M

T

T

S

S

Q

Q

N

N

H

H

G

G

Y

Y

T

A

I

V

E

D

K

L

D

E

S

Q

L

L

E

P

D

D

-

G

-

W

T

S

R

E

L

L

I

F

V

V

T

N

H

-

I

-

A

-

V

A

N

N

D

D

E

G

T

T

V

N

E

E

G

G

V

I

A

V

H

H

L

A

D

S

Y

K

P

P

A

Y

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

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Sites not aligning to the query:

1167 E→K: Severely diminishes UTP inhibition of CPSase; in Su(b).
1883 modified: Phosphoserine
1885 modified: Phosphoserine
1892 modified: Phosphoserine
1894 modified: Phosphoserine

Q18990 Multifunctional protein pyr-1; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Caenorhabditis elegans (see 3 papers)
41% identity, 99% coverage: 1:357/362 of query aligns to 1:365/2198 of Q18990

query
sites
Q18990

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Sites not aligning to the query:

1602 H→Q: In cu8; probable loss of dihydroorotase activity. Severe late stage embryonic lethality in 50% of animals. The few surviving mutants have a shorter and thicker pharyngeal isthmus, an abnormal knobbed tail, mild egg-laying defects, moderate fluid accumulation in the coelom and a slower growth. Actin and intermediate filaments are disorganized in the pharynx. Moderate reduction in heparan sulfate levels and increased levels of chondroitin sulfate. In an umps-1 zu456 mutant background, prevents the formation of abnormally enlarged gut granules in embryos. Complete embryonic lethality in a rnst-2 qx245 mutant background.

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 98% coverage: 5:359/362 of query aligns to 61:450/2244 of Q09794

query
sites
Q09794

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Sites not aligning to the query:

1119 modified: Phosphoserine
1881 modified: Phosphoserine
1885 modified: Phosphoserine

P07756 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Rattus norvegicus (Rat) (see 2 papers)
37% identity, 98% coverage: 5:360/362 of query aligns to 48:406/1500 of P07756

query
sites
P07756

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