SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078430740.1 NCBI__GCF_002019605.1:WP_078430740.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26512 Aspartokinase; Aspartate kinase; EC 2.7.2.4 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see 2 papers)
37% identity, 97% coverage: 1:403/414 of query aligns to 1:410/421 of P26512

query
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P26512

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G277 (= G279) mutation to A: Change in the inhibitory profile upon addition of threonine.
A279 (≠ Q284) mutation to V: Absence of inhibition upon addition of threonine and lysine or lysine alone.
Q298 (vs. gap) mutation to A: Change in the inhibitory profile and absence of dimerization upon addition of threonine.
S301 (≠ N304) mutation to F: Absence of inhibition upon addition of threonine and lysine or lysine alone.; mutation to Y: Feedback-resistant and enhanced expression of the asd gene.
V360 (= V353) mutation to A: Change in the inhibitory profile and shows an different oligomer state upon addition of threonine.
T361 (= T354) mutation to A: Change in the inhibitory profile and absence of dimerization upon addition of threonine.
E363 (≠ K356) mutation to A: Change in the inhibitory profile and absence of dimerization upon addition of threonine.
F364 (≠ I357) mutation to A: Change in the inhibitory profile and shows an different oligomer state upon addition of threonine.

P41398 Aspartokinase; Aspartate kinase; EC 2.7.2.4 from Corynebacterium flavescens (see paper)
37% identity, 97% coverage: 1:403/414 of query aligns to 1:410/421 of P41398

query
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P41398

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D345 (≠ T334) mutation to G: Decreased sensitivity of AK activity to concerted feedback inhibition by lysine and threonine.

P61489 Aspartokinase; Aspartate kinase; AK; ASK; Threonine-sensitive AK; ThrA; EC 2.7.2.4 from Thermus thermophilus (see paper)
37% identity, 97% coverage: 1:402/414 of query aligns to 1:404/405 of P61489

query
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P61489

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K7 (= K7) mutation to A: Loss of aspartokinase activity.; mutation to M: Loss of aspartokinase activity.
G9 (= G9) mutation to M: Loss of aspartokinase activity.
G10 (= G10) mutation to A: Significant decrease in the catalytic efficiency.
S41 (= S41) mutation to A: Significant decrease in the catalytic efficiency. Requires higher concentration of magnesium ion than wild-type.
A42 (= A42) mutation to S: Loss of aspartokinase activity.
T47 (= T52) mutation to A: Significant decrease in the affinity for aspartic acid. Requires higher concentration of magnesium ion than wild-type.
E74 (= E77) mutation to A: Loss of aspartokinase activity.; mutation to Q: Loss of aspartokinase activity.
G135 (= G138) mutation to A: Very low catalytic efficiency.; mutation to S: Loss of aspartokinase activity.
R150 (= R153) mutation to A: Significant decrease in the catalytic efficiency.
D154 (= D157) mutation to A: Significant decrease in the catalytic efficiency. Requires higher concentration of magnesium ion than wild-type.; mutation to N: Significant decrease in the catalytic efficiency. Requires higher concentration of magnesium ion than wild-type.
D174 (= D177) mutation to A: Significant decrease in the catalytic efficiency.
D182 (= D185) mutation to A: Significant decrease in the catalytic efficiency.Requires higher concentration of magnesium ion than wild-type.

3l76A Crystal structure of aspartate kinase from synechocystis (see paper)
36% identity, 96% coverage: 3:398/414 of query aligns to 2:404/585 of 3l76A

query
sites
3l76A

V

L

I

I

V

V

Q

Q

K

K

F

F

G

G

T

T

S

S

V

V

K

G

N

T

E

V

E

E

V

R

R

I

R

Q

M

A

A

V

A

A

R

Q

H

R

V

I

Q

K

K

R

S

T

V

V

N

Q

D

G

G

G

Y

N

K

S

V

L

V

V

S

S

A

A

M

M

G

G

R

K

S

S

G

-

D

-

P

-

Y

-

A

-

T

T

D

D

T

V

L

L

L

V

N

D

L

L

I

A

N

Q

K

Q

-

I

-

S

K

P

T

N

V

P

P

C

K

R

R

R

E

E

Q

M

D

D

L

M

L

L

S

S

C

T

G

G

E

E

I

Q

I

V

S

S

S

I

V

A

V

L

F

L

T

S

D

L

L

A

L

L

S

Q

S

E

Y

I

G

D

V

Q

T

P

A

A

K

I

A

S

M

L

T

T

G

G

A

A

Q

Q

A

V

G

G

F

I

R

V

T

T

N

-

E

-

D

-

F

-

S

-

A

-

K

E

I

I

I

L

E

E

M

I

K

R

C

P

E

D

K

R

L

L

M

E

K

H

T

H

L

L

E

R

K

E

V

G

D

K

A

V

V

V

A

A

G

G

F

F

Q

Q

G

G

Q

I

S

H

K

L

T

-

G

-

E

E

I

I

T

T

L

L

G

G

R

R

G

G

S

S

D

D

T

T

S

S

A

A

T

V

A

A

I

L

G

A

A

A

L

L

G

K

A

A

E

D

W

F

V

C

D

E

I

I

F

Y

T

T

D

D

V

V

E

P

G

G

V

I

M

L

T

T

A

T

D

D

P

P

R

R

I

L

V

V

E

P

G

E

A

A

R

Q

P

L

L

M

S

A

V

E

V

I

T

T

Y

C

N

D

E

E

I

M

C

L

N

E

M

L

A

A

Y

S

Q

L

G

G

A

A

K

K

V

V

I

L

H

H

P

P

R

R

A

A

V

V

E

E

I

I

A

A

M

R

Q

N

A

Y

K

G

I

I

P

P

I

L

H

V

I

V

R

R

S

S

T

S

Y

W

S

S

D

D

S

E

K

P

G

G

T

T

L

K

V

V

T

V

S

A

M

-

V

-

G

-

D

-

Q

-

G

-

Q

P

D

P

V

V

Q

Q

D

N

R

R

L

S

I

L

T

V

G

G

I

L

-

E

-

I

-

A

-

K

-

A

-

V

-

D

-

G

-

V

-

E

-

Y

-

D

A

A

H

D

L

Q

A

A

K

K

V

V

T

A

Q

L

I

L

K

R

V

V

L

P

A

D

K
x
R

E

P

G

G

Q

-

Y

-

D

-

L
x
V

Q
x
A

E

S

K

K

V

L

F

F

K

R

A

D

M

I

A

A

N

Q

E

Q

R

Q

I

V

S
x
D

V
x
I

D
|
D

F

L

I

I

-

I

-
x
Q

-

S

-

I

-

H

N

D

I

G

N

N

P

S

L

N

G

D

V

I

V

A

Y

F

T

T

V

V

M

V

E

K

E

D

V

L

A

L

D

N

R

T

A

A

V

E

E

A

V

V

L

T

-

S

-

A

-

I

-

A

E

P

Q

A

M

L

N

R

Y

S

E

Y

P

P

Q

E

-

A

-

D

-

Q

-

E

-

A

-

E

-

I

V

V

T

E

R

K

R

G

C

I

A

A

K

K

I

I

S

A

A

I

V

A

G

G

A

A

G

G

M
|
M

T

I

G

G

V
x
R

P

P

G

G

V
x
I

T

A

A

A

K

K

I

M

V

F

A

K

A

T

L

L

S

A

K

D

E

V

N

G

V

V

Q
x
N

I
|
I

L

E

Q

M

S

I

A

S

D

T

S
|
S

H
x
E

T

V

T

K

I

V

W

S

V

C

L

V

V

I

K

D

G

Q

E

R

D

D

M

A

V

D

K

R

A

A

V

I

N

A

A

A

L

L

H

S

D

N

M

A

F

F

binding lysine: D286 (≠ S297), I287 (≠ V298), D288 (= D299), M353 (= M347), R356 (≠ V350), I359 (≠ V353), S380 (= S374), E381 (≠ H375)
binding threonine: R269 (≠ K277), V272 (≠ L283), A273 (≠ Q284), Q292 (vs. gap), N373 (≠ Q367), I374 (= I368)

Sites not aligning to the query:

binding lysine: 457, 458, 459, 522, 525, 528, 548, 549, 553
binding threonine: 440, 443, 463, 542, 543

3aawC Crystal structure of aspartate kinase from corynebacterium glutamicum in complex with lysine and threonine (see paper)
36% identity, 97% coverage: 1:400/414 of query aligns to 1:390/392 of 3aawC

query
sites
3aawC

M

M

K

A

V

L

I

V

V

V

Q

Q

K
|
K

F

Y

G

G

T

S

S

S

V

L

K

E

N

S

E

A

E

E

V

R

R

I

R

R

M

N

A

V

A

A

R

E

H

R

V

I

Q

V

K

A

S

T

V

K

N

K

D

A

G

G

Y

N

K

D

V

V

V

C

S
|
S

A

A

M

M

G

G

R

D

S

T

G

-

D

-

P

-

Y

-

A

-

T

T

D

D

T

E

L

L

N

E

L

L

I

A

N

A

-

A

-

V

K

N

K

P

T

V

V

P

P

P

K

A

R

R

E

E

Q

M

D

D

L

M

L

L

S

T

C

A

G

G

E

E

I

R

I

I

S

S

S

N

V

A

V

L

F

V

T

A

D

M

L

A

L

I

S

E

S

S

Y

L

G

G

V

A

T

E

A

A

K

Q

A

S

M

F

T

T

G

G

A

S

Q

Q

A

-

G

-

F

-

R

-

T

-

N

-

E

-

D

D

F

V

S

T

S

P

A

G

K

R

I

V

I

R

E

E

M

-

K

-

C

-

E

-

K

-

L

-

M

-

K

-

T

A

L

L

E

D

K

E

V

G

D

K

A

I

V

C

V

I

V

V

A

A

G

G

F

F

Q

Q

G

G

Q

V

S

N

K

K

T

R

G

-

E

D

I

V

T

T

L

L

G

G

R

R

G

G

G
|
G

S
|
S

D
|
D

T

T

S

T

A

A

T

V

A

A

I

L

G

A

A

A

L

L

G

N

A

A

E

D

W

V

V

C

D

E

I

I

F

Y

T

S

D

D

V

V

E

D

G

G

V

V

M

Y

T

T

A

A

D

D

P

P

R

R

I

I

V

V

E

P

G

N

A

A

R

Q

P

K

L

L

S

E

V

K

V

L

T

S

Y

F

N

E

E

E

I

M

C

L

N

E

M

L

A

A

Y

A

Q

V

G

G

A

S

K

K

V

I

I

L

H

V

P

L

R

R

A

S

V

V

E

E

I

Y

A

A

M

R

Q

A

A

F

K

N

I

V

P

P

I

L

H

R

I

V

R

R

S

S

T

S

Y

Y

S

S

D

N

S

D

K

P

G

G

T

T

L

L

V

I

T

A

S

-

M

-

V

-

G

G

D

S

Q

M

Q

E

G

D

Q

I

D

P

V

V

Q

E

D

E

R

A

L

V

I

L

T

T

G

G

I

V

A

A

-

T

-

D

-

K

H

S

L

E

A

A

K

K

V

V

T

T

Q

V

I

L

K

G

V

I

L

S

A

D

K
|
K

E

P

G
|
G

Q
x
E

Y

-

D

-

L

-

Q
x
A

E

A

K

K

V

V

F

F

K

R

A

A

M

L

A

A

N

D

E

A

R

E

I

I

S

N

V

I

D

D

F

M

I

V

-

L

-
x
Q

N

N

I

V

N

S

P

S

L

V

-

E

-

D

-

G

-

T

G

D

V

I

V

T

Y

F

T

T

V

C

M

P

E

R

E

S

V

D

A

G

D

R

R

R

A

A

V

M

E

E

V

I

L

L

E

K

Q

K

M

L

N

Q

Y

V

E

Q

P

G

Q

N

V

W

T

T

R

N

-

V

-

L

-

Y

-

D

R

Q

C

V

A

G

K

K

I

V

S

S

A

L

V

V

G

G

A

A

G

G

M
|
M

T

K

G

S

V
x
H

P

P

G

G

V

V

T
|
T

A

A

K

E

I

F

V

M

A

E

A

A

L

L

S

R

K

D

E

V

N

N

V

V

Q
x
N

I
|
I

L

E

Q

L

S

I

A

S

D

T

S
|
S

H

E

T

I

T

R

I

I

W

S

V

V

L

L

V

I

K

R

G

E

E

D

D

D

M

L

V

D

K

A

A

A

V

A

N

R

A

A

L

L

H

H

D

E

M

Q

F

F

Q

Q

L

L

binding lysine: K7 (= K7), S41 (= S41), G136 (= G155), S137 (= S156), D138 (= D157), M337 (= M347), H340 (≠ V350), T344 (= T354), S364 (= S374)
binding threonine: K258 (= K277), G260 (= G279), E261 (≠ Q280), A262 (≠ Q284), Q281 (vs. gap), N357 (≠ Q367), I358 (= I368)

5yeiC Mechanistic insight into the regulation of pseudomonas aeruginosa aspartate kinase (see paper)
35% identity, 96% coverage: 3:401/414 of query aligns to 2:396/397 of 5yeiC

query
sites
5yeiC

V

L

I

I

V

V

Q

Q

K

K

F

F

G

G

T

T

S

S

V

V

K

G

N

T

E

V

E

E

V

R

R

I

R

E

M

Q

A

V

A

A

R

E

H

K

V

V

Q

K

K

K

S

F

V

R

N

E

D

A

G

G

Y

D

K

D

V

V

S

S

A

A

M

M

G

-

R

-

S

S

G

G

D

E

P

T

Y

N

A

R

T

L

D

I

T

G

L

L

L

A

N

N

L

Q

I

I

N

M

K

E

K

Q

T

P

V

V

P

P

K

-

R

R

E

E

Q

L

D

D

L

V

L

M

L

V

S

S

C

T

G

G

E

E

I

Q

I

V

S

T

S

I

V

A

V

L

F

L

T

S

D

M

L

A

L

L

S

I

S

K

Y

R

G

G

V

V

T

P

A

A

K

V

A

S

M

Y

T

T

G

G

A

N

Q

Q

A

V

G

R

F

I

R

L

T

T

N

D

E

S

D

A

F

H

S

T

S

K

A

A

K

R

I

I

I

L

E

H

M

I

K

D

C

D

E

T

K

H

L

I

M

R

K

A

T

D

L

L

E

K

K

A

V

G

D

R

A

V

V

V

A

A

G

G

F

F

Q

Q

G

G

Q

V

S

D

K

G

T

N

G

G

E

N

I

I

T

T

L

L

G

G

R

R

G

G

S

S

D

D

T

T

S

T

A

G

T

V

A

A

I

L

G

A

A

A

L

L

G

K

A

A

E

D

W

E

V

C

D

Q

I

I

F

Y

T

T

D

D

V

V

E

D

G

G

V

V

M

Y

T

T

A

T

D

D

P

P

R

R

I

V

V

V

E

P

G

Q

A

A

R

R

P

R

L

L

S

D

V

K

V

I

T

T

Y

F

N

E

E

E

I

M

C

L

N

E

M

M

A

A

Y

S

Q

L

G

G

A

S

K

K

V

V

I

L

H

Q

P

I

R

R

A

A

V

V

E

E

I

F

A

A

M

G

Q

K

A

Y

K

N

I

V

P

P

I

L

H

R

I

V

R

L

S

H

T

S

Y

F

S

Q

D

E

S

G

K

P

G

G

T

T

L

L

V

I

T

T

S

I

M

-

V

-

G

-

D

-

Q

-

G

-

Q

-

D

-

V

-

Q

-

D

D

R

P

L

I

I

I

T

S

G

G

I

I

A

A

H

F

L

N

A

R

K

D

V

E

T

A

Q

K

I

L

K

T

V

I

L

R

A

G

K

V

E

P

G

D

Q
x
T

Y
x
P

D

G

L

V

Q
x
A

E

F

K

K

V

I

F

L

K

G

A

P

M

I

A

S

N

A

E

A

R

N

I

V

S

E

V

V

D

D

F

M

I

I

-

V

-
x
Q

N

N

I

V

-

A

-

H

-

D

N

N

P

T

L

T

G

D

V

F

V

T

Y

F

T

T

V

V

M

H

E

R

E

N

V

D

A

Y

D

L

R

N

A

A

V

L

E

E

V

I

L

L

E

K

Q

Q

M

T

-

A

-

N

-

I

-

G

N

A

Y

R

E

E

P

A

Q

I

V

G

T

D

R

T

R

N

C

I

A

A

K

K

I

V

S

S

A

I

V

V

G

G

A

V

G

G

M
|
M

T

R

G

S

V
x
H

P
x
A

G
|
G

V
|
V

T
x
A

A
x
S

K

R

I

M

V

F

A

E

A

A

L

L

S

A

K

K

E

E

N

S

V

I

Q
x
N

I
|
I

L

Q

Q

M

S

I

A

S

D

T

S

S

H

E

T

I

T

K

I

V

W

S

V

V

L

V

V

I

K

E

G

E

E

K

D

Y

M

L

V

E

K

L

A

A

V

V

N

R

A

A

L

L

H

H

D

T

M

A

F

F

Q

E

L

L

E

D

binding lysine: M342 (= M347), H345 (≠ V350), A346 (≠ P351), G347 (= G352), V348 (= V353), A349 (≠ T354), S350 (≠ A355)
binding threonine: T265 (≠ Q280), P266 (≠ Y281), A269 (≠ Q284), Q288 (vs. gap), N362 (≠ Q367), I363 (= I368)

2hmfA Structure of a threonine sensitive aspartokinase from methanococcus jannaschii complexed with mg-adp and aspartate (see paper)
31% identity, 93% coverage: 12:398/414 of query aligns to 82:461/464 of 2hmfA

query
sites
2hmfA

S

A

V

I

K

K

N

S

E

E

V

I

R

K

R

E

M

E

A

V

A

K

R

K

H

I

V

I

Q

D

K

S

S

R

V

I

N

E

D

E

G

L

Y

E

K

K

V

V

V

L

V

I

V

G

V

V

S

A

A

Y

M

L

G

G

R

-

S

-

G

-

D

-

P

-

Y

-

A

-

T

-

D

-

T

-

L

-

N

-

L

-

I

-

N

-

K

-

K

E

T

L

V

T

P

P

K

K

R

-

E

S

Q

R

D

D

L

Y

L

I

L

L

S

S

C

F

G

G

E

E

I

R

I

L

S

S

V

P

V

I

F

L

T

S

D

G

L

A

L

I

S

R

S

D

Y

L

G

G

V

E

T

K

A

S

K

I

A

A

M

L

T

E

G

G

A

G

Q

E

A

A

G

G

F

I

R

I

T

T

N

D

E

N

D

N

F

F

S

G

S

S

A

A

K

R

I

V

I

K

E

R

M

L

K

E

C

V

-

K

E

E

K

R

L

L

M

L

K

P

T

L

L

L

E

K

K

E

V

G

D

I

A

I

V

P

V

V

A

T

G

G

F
|
F

Q

I

G

G

Q

T

S

T

K

E

T

E

G

G

E

Y

I

I

T

T

L

L

G

G

R
|
R

G
|
G

G

G

S
|
S

D

D

T

Y

S

S

A

A

T

A

A

L

I

I

G

G

A

Y

A

G

L

L

G

D

A

A

E

D

W

I

V

I

D

E

I

I

F

W

T
|
T

D
|
D

V
|
V

E

S

G

G

V

V

M
x
Y

T

T

A
x
T

D
|
D

P
|
P

R
|
R

I

L

V

V

E

P

G

T

A

A

R

R

P

R

L

I

S

P

V

K

V

L

T

S

Y

Y

N

I

E

E

I

A

C

M

N

E

M

L

A

A

Y

Y

Q

F

G

G

A

A

K
|
K

V
|
V

I

L

H

H

P

P

R

R

A

T

V

I

E

E

I

P

A

A

M

M

Q

E

A

K

K

G

I

I

P

P

I

I

H

L

I

V

R

K

S

N

T

T

Y

F

S

E

-

P

D

E

S

S

K

E

G

G

T

T

L

L

V

I

T

T

S

N

M

-

V

-

G

-

D

-

Q

-

G

D

Q

M

D

E

V

M

Q

S

D

D

R

S

L

I

I

V

T

K

G

A

I

I

A

S

H

T

L

I

A

K

K

N

V

V

T

A

Q

L

I

I

K

N

V

I

L

F

A

G

K

A

E

G

-

M

-

V

G

G

Q

V

Y

S

D

G

L

T

Q

A

E

A

K

R

V

I

F

F

K

K

A

A

M

L

A

G

N

E

E

E

R

E

I

V

S

N

V

V

D

I

F

L

I

I

N

S

-

Q

-

G

I

S

N

S

P

E

L

T

G

N

V

I

V

S

Y

L

T

V

V

V

M

S

E

E

V

D

A

V

D

D

R

K

A

A

V

L

E

K

V

A

L

L

E

K

Q

R

-

E

-

F

-

G

-

D

-

F

-

L

-

N

M

L

N

I

Y

R

E

D

P

V

Q

S

V

V

T

D

R

K

R

D

C

V

A

C

K

V

I

I

S

S

A

V

V

V

G

G

A

A

G

G

M

M

T

R

G

G

V

A

P

K

G

G

V

I

T

A

A

G

K

K

I

I

V

F

A

T

A

A

L

V

S

S

K

E

E

S

-

G

-

A

N

N

V

I

Q

K

I

M

L

I

Q

A

S

Q

A

G

D

S

S

S

H

E

T

V

T

N

I

I

W

S

V

F

L

V

V

I

K

D

G

E

E

K

D

D

M

L

V

L

K

N

A

C

V

V

N

R

A

K

L

L

H

H

D

E

M

K

F

F

binding adenosine-5'-diphosphate: T229 (= T176), D230 (= D177), V231 (= V178), Y235 (≠ M182), T237 (≠ A184), D238 (= D185), P239 (= P186), R240 (= R187), K265 (= K212), V266 (= V213)
binding aspartic acid: F192 (= F139), R206 (= R153), G207 (= G154), S209 (= S156)

Sites not aligning to the query:

3c1mC Cyrstal structure of threonine-sensitive aspartokinase from methanococcus jannaschii with mgamp-pnp and l-aspartate (see paper)
31% identity, 93% coverage: 12:398/414 of query aligns to 82:465/468 of 3c1mC

query
sites
3c1mC

S

A

V

I

K

K

N

S

E

E

V

I

R

K

R

E

M

E

A

V

A

K

R

K

H

I

V

I

Q

D

K

S

S

R

V

I

N

E

D

E

G

L

Y

E

K

K

V

V

V

L

V

I

V

G

V

V

S

A

A

Y

M

L

G

G

R

-

S

-

G

-

D

-

P

-

Y

-

A

-

T

-

D

-

T

-

L

-

N

-

L

-

I

-

N

-

K

-

K

E

T

L

V

T

P

P

K

K

R

-

E

S

Q

R

D

D

L

Y

L

I

L

L

S

S

C

F

G

G

E
|
E

I

R

I

L

S

S

V

P

V

I

F

L

T

S

D

G

L

A

L

I

S

R

S

D

Y

L

G

G

V

E

T

K

A

S

K

I

A

A

M

L

T

E

G

G

A

G

Q

E

A

A

G

G

F

I

R

I

T

T

N

D

E

N

D

N

F

F

S

G

S

S

A

A

K

R

I

V

I

K

E

R

M

L

K

E

C

V

-

K

E

E

K

R

L

L

M

L

K

P

T

L

L

L

E

K

K

E

V

G

D

I

A

I

V

P

V

V

A

T

G

G

F
|
F

Q

I

G

G

Q

T

S

T

K

E

T

E

G

G

E

Y

I

I

T

T

L

L

G

G

R
|
R

G
|
G

G

G

S
|
S

D

D

T

Y

S

S

A

A

T

A

A

L

I

I

G

G

A

Y

A

G

L

L

G

D

A

A

E

D

W

I

V

I

D

E

I

I

F

W

T
|
T

D
|
D

V

V

E

S

G

G

V

V

M
x
Y

T

T

A

T

D
|
D

P
|
P

R
|
R

I

L

V

V

E

P

G

T

A

A

R

R

P

R

L

I

S

P

V

K

V

L

T

S

Y

Y

N

I

E

E

I

A

C

M

N

E

M

L

A

A

Y

Y

Q

F

G

G

A

A

K
|
K

V
|
V

I

L

H

H

P

P

R

R

A

T

V

I

E

E

I

P

A

A

M

M

Q

E

A

K

K

G

I

I

P

P

I

I

H

L

I

V

R

K

S

N

T

T

Y

F

S

E

-

P

D

E

S

S

K

E

G

G

T

T

L

L

V

I

T

T

S

N

M

-

V

-

G

-

D

-

Q

-

G

D

Q

M

D

E

V

M

Q

S

D

D

R

S

L

I

I

V

T

K

G

A

I

I

A

S

H

T

L

I

A

K

K

N

V

V

T

A

Q

L

I

I

K

N

V

I

L

F

A

G

K

A

E

G

-

M

-

V

G

G

Q

V

Y

S

D

G

L

T

Q

A

E

A

K

R

V

I

F

F

K

K

A

A

M

L

A

G

N

E

E

E

R

E

I

V

S

N

V

V

D

I

F

L

I

I

N

S

-

Q

-

G

I

S

N

S

P

E

L

T

G

N

V

I

V

S

Y

L

T

V

V

V

M

S

E

E

V

D

A

V

D

D

R

K

A

A

V

L

E

K

V

A

L

L

E

K

Q

R

-

E

-

F

-

G

-

D

-

G

-

K

-

S

-

F

-

L

-

N

M

L

N

I

Y

R

E

D

P

V

Q

S

V

V

T

D

R

K

R

D

C

V

A

C

K

V

I

I

S

S

A

V

V

V

G

G

A

A

G

G

M

M

T

R

G

G

V

A

P

K

G

G

V

I

T

A

A

G

K

K

I

I

V

F

A

T

A

A

L

V

S

S

K

E

E

S

-

G

-

A

N

N

V

I

Q

K

I

M

L

I

Q

A

S

Q

A

G

D

S

S

S

H

E

T

V

T

N

I

I

W

S

V

F

L

V

V

I

K

D

G

E

E

K

D

D

M

L

V

L

K

N

A

C

V

V

N

R

A

K

L

L

H

H

D

E

M

K

F

F

binding phosphoaminophosphonic acid-adenylate ester: T229 (= T176), D230 (= D177), Y235 (≠ M182), D238 (= D185), P239 (= P186), R240 (= R187), K265 (= K212), V266 (= V213)
binding aspartic acid: E129 (= E77), F192 (= F139), R206 (= R153), G207 (= G154), S209 (= S156)

Sites not aligning to the query:

3c1nA Crystal structure of allosteric inhibition threonine-sensitive aspartokinase from methanococcus jannaschii with l-threonine (see paper)
33% identity, 80% coverage: 67:398/414 of query aligns to 118:456/458 of 3c1nA

query
sites
3c1nA

R

K

E

S

Q

R

D

D

L

Y

L

I

L

L

S

S

C

F

G

G

E

E

I

R

I

L

S

S

V

P

V

I

F

L

T

S

D

G

L

A

L

I

S

R

S

D

Y

L

G

G

V

E

T

K

A

S

K

I

A

A

M

L

T

E

G

G

A

G

Q

E

A

A

G

G

F

I

R

I

T

T

N

D

E

N

D

N

F

F

S

G

S

S

A

A

K

R

I

V

I

K

E

R

M

L

K

E

C

V

-

K

E

E

K

R

L

L

M

L

K

P

T

L

L

L

E

K

K

E

V

G

D

I

A

I

V

P

V

V

A

T

G

G

F

F

Q

I

G

G

Q

T

S

T

K

E

T

E

G

G

E

Y

I

I

T

T

L

L

G

G

R

R

G

G

S

S

D

D

T

Y

S

S

A

A

T

A

A

L

I

I

G

G

A

Y

A

G

L

L

G

D

A

A

E

D

W

I

V

I

D

E

I

I

F
x
W

T
|
T

D
|
D

V

V

E

S

G

G

V

V

M

Y

T

T

A

T

D

D

P

P

R

R

I

L

V

V

E

P

G

T

A

A

R

R

P

R

L

I

S

P

V

K

V

L

T

S

Y

Y

N

I

E

E

I

A

C

M

N

E

M

L

A

A

Y

Y

Q

F

G

G

A

A

K

K

V

V

I

L

H

H

P

P

R

R

A

T

V

I

E

E

I

P

A

A

M

M

Q

E

A

K

K

G

I

I

P

P

I

I

H

L

I

V

R

K

S

N

T

T

Y

F

S

E

-

P

D

E

S

S

K

E

G

G

T

T

L

L

V

I

T

T

S

N

M

D

V

M

G

-

D

-

Q

-

G

-

Q

-

D

-

V

-

Q

-

D

D

R

S

L

I

I

V

T

K

G

A

I

I

A

S

H

T

L

I

A

K

K

N

V

V

T

A

Q

L

I

I

K

N

V

I

L

F

A

G

K

A

E

G

-

M

-

V

G

G

Q

V

Y

S

D

G

L

T

Q

A

E

A

K

R

V

I

F

F

K

K

A

A

M

L

A

G

N

E

E

E

R

E

I

V

S

N

V

V

D

I

F

L

I

I

N

S

-

Q

-

G

I

S

N

S

P

E

L

T

G

N

V

I

V

S

Y

L

T

V

V

V

M

S

E

E

V

D

A

V

D

D

R

K

A

A

V

L

E

K

V

A

L

L

E

K

Q

R

-

E

-

F

-

G

-

D

-

L

-

N

M

L

N

I

Y

R

E

D

P

V

Q

S

V

V

T

D

R

K

R

D

C

V

A

C

K

V

I

I

S

S

A

V

V

V

G

G

A

A

G

G

M

M

T

R

G

G

V
x
A

P

K

G

G

V
x
I

T
x
A

A

G

K

K

I

I

V

F

A

T

A

A

L

V

S

S

K

E

E

S

-

G

-

A

N
|
N

V
x
I

Q

K

I

M

L

I

Q

A

S
x
Q

A

G

D

S

S

S

H
x
E

T

V

T

N

I

I

W

S

V

F

L

V

V

I

K

D

G

E

E

K

D

D

M

L

V

L

K

N

A

C

V

V

N

R

A

K

L

L

H

H

D

E

M

K

F

F

binding threonine: W227 (≠ F175), T228 (= T176), D229 (= D177), A406 (≠ V350), I409 (≠ V353), A410 (≠ T354), N423 (= N365), I424 (≠ V366), Q429 (≠ S371), E433 (≠ H375)

Sites not aligning to the query:

binding threonine: 7, 8, 9, 10

3ab4A Crystal structure of feedback inhibition resistant mutant of aspartate kinase from corynebacterium glutamicum in complex with lysine and threonine (see paper)
33% identity, 96% coverage: 3:400/414 of query aligns to 2:369/370 of 3ab4A

query
sites
3ab4A

V

L

I

V

V

V

Q

Q

K

K

F

Y

G

G

T

S

S

S

V

L

K

E

N

S

E

A

E

E

V

R

R

I

R

R

M

N

A

V

A

A

R

E

H

R

V

I

Q

V

K

A

S

T

V

K

N

K

D

A

G

G

Y

N

K

D

V

V

V

C

S

S

A

A

M

M

G

G

R

D

S

T

G

-

D

-

P

-

Y

-

A

-

T

T

D

D

T

E

L

L

N

E

L

L

I

A

N

A

-

A

-

V

K

N

K

P

T

V

V

P

P

P

K

A

R

R

E

E

Q

M

D

D

L

M

L

L

S

T

C

A

G

G

E

E

I

R

I

I

S

S

S

N

V

A

V

L

F

V

T

A

D

M

L

A

L

I

S

E

S

S

Y

L

G

G

V

A

T

E

A

A

K

Q

A

S

M

F

T

T

G

G

A

S

Q

V

A

T

G

P

F

G

R

R

T

V

N

R

E

E

D

A

F

L

S

D

S

E

A

G

K

K

I

I

I

C

E

I

M

V

K

-

C

-

E

-

K

-

L

-

M

-

K

-

T

-

L

-

E

-

K

-

V

-

D

-

A

-

V

-

A

-

G

-

F

-

Q

-

G

-

Q

-

S

-

K

-

T

-

G

-

E

-

I

-

T

-

L

-

G

-

R

-

G

G

S

S

D

D

T

T

S

T

A

A

T

V

A

A

I

L

G

A

A

A

L

L

G

N

A

A

E

D

W

V

V

C

D

E

I

I

F

Y

T

S

D

D

V

V

E

D

G

G

V

V

M

Y

T

T

A

A

D

D

P

P

R

R

I

I

V

V

E

P

G

N

A

A

R

Q

P

K

L

L

S

E

V

K

V

L

T

S

Y

F

N

E

E

E

I

M

C

L

N

E

M

L

A

A

Y

A

Q

V

G

G

A

S

K

K

V

I

I

L

H

V

P

L

R

R

A

S

V

V

E

E

I

Y

A

A

M

R

Q

A

A

F

K

N

I

V

P

P

I

L

H

R

I

V

R

R

S

S

T

S

Y

Y

S

S

D

N

S

D

K

P

G

G

T

T

L

L

V

I

T

A

S

-

M

-

V

-

G

G

D

S

Q

M

Q

E

G

D

Q

I

D

P

V

V

Q

E

D

E

R

A

L

V

I

L

T

T

G

G

I

V

A

A

-

T

-

D

-

K

H

S

L

E

A

A

K

K

V

V

T

T

Q

V

I

L

K

G

V

I

L

S

A

D

K
|
K

E

P

G
|
G

Q
x
E

Y

-

D

-

L

-

Q
x
A

E

A

K

K

V

V

F

F

K

R

A

A

M

L

A

A

N

D

E

A

R

E

I

I

S

N

V

I

D

D

F

M

I

V

N

L

I
x
Q

N

N

P

V

L

F

G

S

-

D

-

G

-

T

-

D

V

I

V

T

Y

F

T

T

V

C

M

P

E

R

E

S

V

D

A

G

D

R

R

R

A

A

V

M

E

E

V

I

L

L

E

K

Q

K

M

L

N

Q

Y

V

E

Q

P

G

Q

N

V

W

T

T

R

N

-

V

-

L

-

Y

-

D

R

Q

C

V

A

G

K

K

I

V

S

S

A

L

V

V

G

G

A

A

G

G

M
|
M

T

K

G

S

V
x
H

P
|
P

G

G

V
|
V

T
|
T

A

A

K

E

I

F

V

M

A

E

A

A

L

L

S

R

K

D

E

V

N

N

V

V

Q
x
N

I
|
I

L

E

Q

L

S

I

A

S

D

T

S
|
S

H
x
E

T

I

T

R

I

I

W

S

V

V

L

L

V

I

K

R

G

E

E

D

D

D

M

L

V

D

K

A

A

A

V

A

N

R

A

A

L

L

H

H

D

E

M

Q

F

F

Q

Q

L

L

binding lysine: M316 (= M347), H319 (≠ V350), P320 (= P351), V322 (= V353), T323 (= T354), S343 (= S374), E344 (≠ H375)
binding threonine: K239 (= K277), G241 (= G279), E242 (≠ Q280), A243 (≠ Q284), Q262 (≠ I303), N336 (≠ Q367), I337 (= I368)

2cdqA Crystal structure of arabidopsis thaliana aspartate kinase complexed with lysine and s- adenosylmethionine (see paper)
30% identity, 81% coverage: 62:398/414 of query aligns to 109:458/470 of 2cdqA

query
sites
2cdqA

K

K

T

E

V

L

P

T

K

L

R

R

E

T

Q

R

D

D

L

Y

L

L

L

V

S

S

C

F

G

G

E
|
E

I

C

I

L

S

S

S

T

V

R

V

I

F

F

T

A

D

A

L

Y

L

L

S

N

S

T

Y

I

G

G

V

V

T

K

A

A

K

R

A

Q

M

Y

T

D

G

A

A

F

Q

E

A

I

G

G

F

F

R

I

T

T

N

T

E

D

D

D

F

F

S

T

S

N

A

G

K

D

I

I

I

L

E

E

M

A

K

T

C

Y

E

P

K

A

L

V

M

A

K

K

T

R

L

L

-

Y

-

D

-

W

-

M

E

H

K

D

V

P

D

A

A

V

V

P

V

I

V

V

A

T

G

G

F

F

Q

L

G

G

Q

K

S

G

-

W

K

K

T

T

G

G

E

A

I

V

T

T

L

L

G

G

R

R

G

G

S

S

D

D

T

L

S

T

A

A

T

T

A

T

I

I

G

G

A

K

A

A

L

L

G

G

A

L

E

K

W

E

V

I

D

Q

I

V

F

W

T

K

D

D

V

V

E

D

G

G

V

V

M

L

T

T

A

C

D

D

P

P

R

T

I

I

V

Y

E

K

G

R

A

A

R

T

P

P

L

V

S

P

V

Y

V

L

T

T

Y

F

N

D

E

E

I

A

C

A

N

E

M

L

A

A

Y

Y

Q

F

G

G

A

A

K

Q

V

V

I

L

H

H

P

P

R

Q

A

S

V

M

E

R

I

P

A

A

M

R

Q

E

A

G

K

E

I

I

P

P

I

V

H

R

I

V

R

K

S

N

T

S

Y

Y

S

N

-

P

D

K

S

A

K

P

G

G

T

T

L

I

V

I

T

T

S

-

M

-

V

-

G

-

D

-

Q

-

Q

K

G

T

Q

R

D

D

V

M

Q

T

D

K

R

S

L

I

I

L

T

T

G

S

I

I

A

V

H

L

L

K

A

R

K

N

V

V

T

T

Q

M

I

L

K

D

V

I

L

A

A

S

K

T

E

R

-
x
M

-

L

G

G

Q
|
Q

Y

V

D

G

L
x
F

Q
x
L

E

A

K

K

V

V

F

F

K

S

A

I

M

F

A

E

N

E

E

L

R
x
G

I
|
I

S
|
S

V
|
V

D
|
D

F

V

I

V

N

A

I

T

N

S

P

E

L

V

G

S

V

I

V

S

Y

L

T

T

-

L

-

D

-

P

-

S

-

K

-

L

-
x
W

-
x
S

-
x
R

-

E

V
x
L

M

I

E

Q

V
x
E

A

L

D

D

R

H

A

V

V

V

E

E

V

E

L

L

E

E

Q

K

M

I

N

A

Y

V

E

V

P

N

Q

L

V

L

T

K

R

G

R

R

C

-

A

A

K

I

I

I

S

S

A

L

V

I

G

G

A

-

G

N

M

V

T

Q

G

H

V

S

P

S

G

L

V

I

T

L

A

E

K

R

I

A

V

F

A

H

A

V

L

L

S

Y

K

T

E

K

-

G

-

V

N

N

V

V

Q

Q

I

M

L

I

Q

S

S

Q

A

G

D

A

S

S

H

K

T

V

T

N

I

I

W

S

V

F

L

I

V

V

K

N

G

E

E

A

D

E

M

A

V

E

K

G

A

C

V

V

N

Q

A

A

L

L

H

H

D

K

M

S

F

F

binding lysine: E124 (= E77), M327 (vs. gap), Q330 (= Q280), F333 (≠ L283), L334 (≠ Q284), S347 (= S297), V348 (= V298), D349 (= D299)
binding s-adenosylmethionine: G345 (≠ R295), I346 (= I296), S347 (= S297), W368 (vs. gap), S369 (vs. gap), R370 (vs. gap), L372 (≠ V312), E376 (≠ V316)

Sites not aligning to the query:

binding lysine: 40, 41, 46

3tviE Crystal structure of clostridium acetobutylicum aspartate kinase (caak): an important allosteric enzyme for industrial amino acids production (see paper)
28% identity, 96% coverage: 1:399/414 of query aligns to 1:437/439 of 3tviE

query
sites
3tviE

M

L

K

K

V

I

I

V

V

V

Q

T

K

K

F

F

G

G

T

S

S

S

V

L

K

A

N

D

E

S

E

N

V

Q

R

F

R

K

M

K

A

V

A

K

R

G

H

I

V

I

Q

D

K

S

S

D

V

A

N

N

D

R

G

K

Y

Y

K

-

V

-

V

I

V

P

S

S

A

A

M

P

G

G

-

K

R

R

S

T

G

N

D

K

P

D

Y

Y

A

K

T

I

D
x
T

T

D

L

L

N

Y

L

L

I

C

N

N

-

A

-

H

-

V

K

K

K

N

T

G

V

I

P

P

K

F

R

D

E

D

Q

V

D

F

L

K

L

L

L

I

S

S

-

Q

-

R

C

Y

G

T

E

E

I

I

I

V

S

S

S

E

V

L

V

N

F

I

T

D

D

M

L

D

L

I

S

A

S

Y

Y

Y

-

L

-

E

G

K

V

V

T

K

A

K

K

N

A

I

M

E

T

N

G

G

A

A

Q

S

A

S

G

D

F

Y

R

A

T

A

-

S

-

R

-

G

-
x
E

-

Y

-

L

-

N

-

G

-

V

-

I

-

L

-

A

-

K

-

Y

-

L

N

N

E

A

D

E

F

F

-

I

S

D

S

A

A

A

K

E

I

V

I

I

E

F

M

F

K

D