SitesBLAST
Comparing WP_078715956.1 NCBI__GCF_900167125.1:WP_078715956.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
55% identity, 99% coverage: 3:247/247 of query aligns to 2:246/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
56% identity, 97% coverage: 8:247/247 of query aligns to 4:239/239 of 3sj7A
- active site: G12 (= G16), S138 (= S142), Q148 (= Q152), Y151 (= Y155), K155 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (= S14), R11 (= R15), I13 (= I17), N31 (≠ T35), Y32 (= Y36), A33 (≠ V37), G34 (≠ S38), S35 (≠ K39), A58 (≠ L62), N59 (≠ D63), V60 (≠ S64), N86 (= N90), A87 (= A91), T109 (≠ I113), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
55% identity, 98% coverage: 7:247/247 of query aligns to 7:247/247 of 4jroC
- active site: G16 (= G16), S142 (= S142), Q152 (= Q152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (= S14), R15 (= R15), G16 (= G16), I17 (= I17), N35 (≠ T35), Y36 (= Y36), N37 (≠ V37), G38 (≠ S38), S39 (≠ K39), N63 (≠ D63), V64 (≠ S64), N90 (= N90), A91 (= A91), I93 (= I93), I113 (= I113), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (= I188), T190 (= T190)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
53% identity, 98% coverage: 7:247/247 of query aligns to 8:246/247 of P73574
- A14 (≠ G13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ A150) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K159) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (= F187) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ T200) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
52% identity, 98% coverage: 7:247/247 of query aligns to 10:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (= S14), R18 (= R15), I20 (= I17), T40 (≠ V37), N62 (≠ D63), V63 (≠ S64), N89 (= N90), A90 (= A91), I92 (= I93), V139 (≠ I140), S141 (= S142), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), I187 (= I188), T189 (= T190), M191 (= M192)
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
57% identity, 98% coverage: 7:247/247 of query aligns to 7:239/240 of 4dmmB
- active site: G16 (= G16), S142 (= S142), Q152 (= Q152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (= S14), R15 (= R15), G16 (= G16), I17 (= I17), A37 (≠ V37), S38 (= S38), S39 (≠ K39), A62 (≠ L62), D63 (= D63), V64 (≠ S64), N90 (= N90), A91 (= A91), L113 (≠ I113), I140 (= I140), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), G186 (= G186), I188 (= I188), T190 (= T190), M192 (= M192)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
49% identity, 100% coverage: 1:247/247 of query aligns to 1:243/244 of 6t77A
- active site: G16 (= G16), S138 (= S142), Y151 (= Y155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (= S14), R15 (= R15), T37 (≠ V37), L58 (= L62), N59 (≠ D63), V60 (≠ S64), A87 (= A91), G88 (= G92), I89 (= I93)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
50% identity, 98% coverage: 7:247/247 of query aligns to 7:243/244 of P0AEK2
- GASR 12:15 (≠ GGSR 12:15) binding NADP(+)
- T37 (≠ V37) binding NADP(+)
- NV 59:60 (≠ DS 63:64) binding NADP(+)
- N86 (= N90) binding NADP(+)
- Y151 (= Y155) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YVAAK 155:159) binding NADP(+)
- A154 (= A158) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K159) mutation to A: Defect in the affinity for NADPH.
- I184 (= I188) binding NADP(+)
- E233 (≠ Q237) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
50% identity, 98% coverage: 7:247/247 of query aligns to 6:242/243 of 1q7bA
- active site: G15 (= G16), E101 (≠ D106), S137 (= S142), Q147 (= Q152), Y150 (= Y155), K154 (= K159)
- binding calcium ion: E232 (≠ Q237), T233 (≠ V238)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (= S14), R14 (= R15), T36 (≠ V37), N58 (≠ D63), V59 (≠ S64), N85 (= N90), A86 (= A91), G87 (= G92), I88 (= I93), S137 (= S142), Y150 (= Y155), K154 (= K159), P180 (= P185), G181 (= G186), I183 (= I188)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
51% identity, 98% coverage: 7:247/247 of query aligns to 10:242/243 of 4i08A
- active site: G19 (= G16), N113 (= N114), S141 (= S142), Q151 (= Q152), Y154 (= Y155), K158 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (= S14), R18 (= R15), I20 (= I17), T40 (≠ V37), N62 (≠ D63), V63 (≠ S64), N89 (= N90), A90 (= A91), G140 (≠ S141), S141 (= S142), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), T189 (= T190)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
50% identity, 97% coverage: 7:245/247 of query aligns to 3:242/244 of 1edoA
- active site: G12 (= G16), S138 (= S142), Y151 (= Y155), K155 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (= S14), R11 (= R15), I13 (= I17), N31 (≠ T35), Y32 (= Y36), A33 (≠ V37), R34 (≠ S38), S35 (≠ K39), D59 (= D63), V60 (≠ S64), N86 (= N90), A87 (= A91), S138 (= S142), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186), I184 (= I188), S186 (≠ T190), M188 (= M192)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
49% identity, 100% coverage: 1:247/247 of query aligns to 1:243/244 of P0A2C9
- M125 (= M129) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A227) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (≠ G228) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
49% identity, 98% coverage: 7:247/247 of query aligns to 6:242/243 of 1q7cA
- active site: G15 (= G16), S137 (= S142), Q147 (= Q152), F150 (≠ Y155), K154 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (= S14), R14 (= R15), A35 (≠ Y36), T36 (≠ V37), L57 (= L62), N58 (≠ D63), V59 (≠ S64), G87 (= G92), I88 (= I93)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
49% identity, 98% coverage: 7:247/247 of query aligns to 6:242/243 of 7emgB
4ag3A Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with NADPH at 1.8a resolution (see paper)
49% identity, 100% coverage: 1:247/247 of query aligns to 8:253/254 of 4ag3A
- active site: G23 (= G16), S148 (= S142), Y161 (= Y155), K165 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G12), S21 (= S14), R22 (= R15), G23 (= G16), I24 (= I17), T44 (≠ V37), L68 (= L62), D69 (= D63), V70 (≠ S64), N96 (= N90), A97 (= A91), I146 (= I140), S148 (= S142), Y161 (= Y155), K165 (= K159), P191 (= P185), G192 (= G186), F193 (= F187), I194 (= I188), T196 (= T190), M198 (= M192), T199 (= T193)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
45% identity, 100% coverage: 1:247/247 of query aligns to 1:243/244 of 6wprA
- active site: G16 (= G16), S138 (= S142), Y151 (= Y155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (= S14), R15 (= R15), T37 (≠ A42), L58 (= L62), D59 (= D63), V60 (≠ S64), N86 (= N90), A87 (= A91), G88 (= G92), I89 (= I93), I136 (= I140), Y151 (= Y155), K155 (= K159), P181 (= P185)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
45% identity, 100% coverage: 1:247/247 of query aligns to 2:244/244 of 4nbuB