SitesBLAST

Q96XT2 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
30% identity, 99% coverage: 6:383/383 of query aligns to 225:614/628 of Q96XT2

query
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Q96XT2

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T257 (= T38) binding substrate
R344 (= R115) binding substrate

Q96Y66 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
31% identity, 98% coverage: 7:383/383 of query aligns to 225:613/627 of Q96Y66

query
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Q96Y66

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T349 (= T120) mutation to L: Same oxidoreductase activity as the wild-type.
D468 (≠ L238) mutation to A: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.

Sites not aligning to the query:

41 S→A: Same oxidoreductase activity as the wild-type.

P72578 2-oxoacid:ferredoxin oxidoreductase subunit alpha; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see 2 papers)
31% identity, 98% coverage: 7:383/383 of query aligns to 225:613/632 of P72578

query
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P72578

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Y253 (= Y35) mutation Y->A,W: Loss of oxidoreductase activity.; mutation to F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
YPITP 253:257 (= YPITP 35:39) YPITP motif
P254 (= P36) mutation to G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
I255 (= I37) mutation I->L,M,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
T256 (= T38) mutation T->A,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
P257 (= P39) mutation P->A,G,V: Loss of oxidoreductase activity.
R344 (= R115) mutation R->A,K: Loss of oxidoreductase activity.
T353 (= T124) Plays a key role in the broad substrate specificity; mutation T->I,V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.

9bt4A Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
25% identity, 88% coverage: 8:344/383 of query aligns to 7:331/402 of 9bt4A

query
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9bt4A

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Q

Q

R

F

L

M

P

A

K

D

T

G

E

E

-

I

-

P

T

N

G

C

T

E

F

Y

L

I

Q

N

M

V

E

E

D

S

E
|
E

I

F

A

S

S

A

M

I

G

S

A

A

V

L

I

V

G

G

A

A

S

S

L

A

A

V

G

G

C

A

K

R

A

T

M

Y

T

S

A

A

T

T

S

T

G

S

P

Q

G

G

F

L

S

L

L

L

M

M

Q

H

E

E

H

V

L

L

G

F

Y

N

A

A

C

A

M

G

V

M

E

R

A

L

P

P

L

I

V

V

L

M

V

T

N

V

V

A

M

N

R

R

G

A

G

V

P

S

S

A

T

P

G

I

L

N

P

I

T

W

S

N

P

D

A

H

Q

Q

G

D

D

S

V

I

Q

A

Q

Q

A

R

R

-

W

-

G

-

T

-

H

-

G

-

D

D

H

T

A

G

I

W

L

L

V

Q

L

L

S

Y

A

A

S

E

N

D

V

V

Q

Q

E

E

C

A

L

A

E

D

M

M

T

V

I

P

T

Q

A

I

F

F

N

K

L

I

A

A

E

E

-

D

-

K

K

D

F

V

R

L

T

L

P

P

V

G

I

M

L

A

L

C

L

M

D

D

E

G

-

F

I

I

T

L

A

S

H

H

T

V

R

Y

E

E

K

P

I

V

V

V

I

L

P

L

R

E

E

Q

D

D

E

L

Y

T

E

D

V

E

F

F

N

-

R

-

V

-

V

L

P

P

T

K

M

Y

P

E

P

P

E

E

W

Y

-

V

Y

L

K

D

P

P

F

K

E

N

E

P

T

L

V

T

R

F

G

G

V

A

P

F

P

A

M

D

P

P

P

S

I

T

G

Y

S

T

G

E

Y

F

R

R

F

Y

H

-

V

-

T

-

G

-

L

L

T

Q

H

E

D

K

P

A

N

M

G

Q

F

A

P

A

T

L

S

P

R

K

P

I

E

E

E

A

V

V

A

S

D

K

L

E

M

F

D

A

R

E

M

I

F

Y

R

G

K

R

V

-

D

-

Q

-

F

-

L

-

P

D

Q

H

V

G

S

G

L

L

H

I

E

D

E

G

F

Y

R

Q

C

L

E

E

D

D

A

A

E

E

V

V

C

V

V

I

V

M

A

A

Y

M

G

G

S

S

V

L

A

V

R

G

S

T

A

L

E

K

Y

D

A

V

V

V

E

D

Q

R

A

Y

R

R

E

A

R

K

G

G

V

E

K

K

A

I

G

G

L

I

L

L

K

K

L

V

K

R

T

S

L

F

Y

R

P

P

Y

F

P

P

R

K

A

M

A

Q

T

I

E

R

K

K

V

A

M

L

G

A

H

N

A

A

R

N

A

A

L

V

I

V

V

V

P

L

E

D

M

K

N

N

M

I

G

S

binding thiamine diphosphate: P35 (= P36), I36 (= I37), E66 (= E65)

5exeA Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
24% identity, 96% coverage: 16:383/383 of query aligns to 8:374/394 of 5exeA

query
sites
5exeA

G

G

N

C

E

V

A

A

V

V

V

A

E

H

G

G

A

V

L

R

L

L

A

A

G

D

C

V

S

D

F

V

Y

I

G

C

G

S

Y
|
Y

P
|
P

I
|
I

T
x
R

P

P

S

Y

S

T

E

G

I

I

M

M

E

S

G

E

M

L

A

A

Q

R

R

M

L

V

P

A

K

D

T

G

E

E

-

L

T

D

G

A

T

E

F

F

L

V

Q

H

M

G

E

E

D

G

E
|
E

I

H

A

A

S

Q

M

L

G

S

A

V

V

V

I

Y

G

G

A

A

S

S

L

A

A

A

G

G

C

A

K

R

A

V

M

F

T

T

A

G

T

S

S

S

G

G

P
x
V

G

G

F

V

S

T

L

-

M

-

Q

-

E

-

H

-

L

-

G

-

Y

Y

A

A

C

M

M

E

V

V

E

Y

A

S

P

P

L

I

V

-

L

-

V

-

N

-

V

-

M

-

R

-

G

-

P

S

S

G

T

E

G

R

L

L

P

P

T

V

S

Q

P

M

A

A

Q

I

G

A

D

D

V
x
R

Q

T

Q

L

A

D

R

P

W

P

G

G

T

D

H
x
F

G

G

-

E

-

H

-

T

-

D

-

A

-

E

-

C

-

R

D

D

H

Q

A

G

I

W

L

I

V

Q

L

G

S

W

A

A

S

S

N

T

V

P

Q

Q

E

E

C

A

L

L

E

D

M

N

T

T

I

L

T

I

A

Y

F

Y

N

R

L

V

A

G

E

E

K

D

F

Q

R

R

T

V

-

L

P

P

V

Q

I

Y

L

A

L

C

L

L

D

D

-

G

E

Y

I

F

T

V

A

S

H

H

T

I

R

L

E

G

K

P

I

V

V

D

I

I

P

P

R

D

E

E

D

A

E

Q

Y

V

E

K

V

E

F

F

-

L

-

P

-

Y

-

K

-

N

N

H

R

H

V

V

V

L

P

D

T

P

M

R

P

K

P

P

E

Q

W

I

Y

I

K

G

P

P

F

Q

E

I

E

E

T

-

V

-

R

-

G

-

V

-

P

P

P

A

M

M

P

G

P

P

I

P

G

L

S

Q

G

Y

Y

Q

R

R

F

Y

H

Q

V

A

T

V

G

K

L

G

T

V

H

H

D

-

P

-

N

-

G

-

F

-

P

-

T

-

S

K

R

V

P

L

E

E

V

A

A

C

D

D

L

E

M

F

D

A

R

R

M

I

F

F

-

G

R

R

K

K

V

Y

D

D

Q

P

F

Y

L

L

P

-

Q

-

V

-

S

-

L

-

H

-

E

D

E

E

F

Y

R

L

C

T

E

D

D

D

A

A

E

E

V

V

C

I

V

I

V

F

A

G

Y

Q

G

G

S

A

V

H

A

M

R

E

S

T

A

A

E

K

Y

A

A

V

V

A

E

R

Q

R

A

L

R

R

E

N

R

L

G

G

V

E

K

K

A

V

G

G

L

V

L

A

K

R

L

L

K

R

T

T

L

F

Y

R

P

P

Y

F

P

P

R

T

A

E

A

Q

T

I

E

K

K

E

V

R

M

L

G

S

H

K

A

F

R

K

A

A

L

I

I

G

V

V

P

L

E

D

M

V

N

S

-

A

-

N

-

F

-

G

-

I

-

S

-

C

-

S

-

G

-

V

-

L

M

L

G

S

Q

E

I

L

S

R

R

A

E

A

V

L

K

Y

R

D

V

Y

N

G

N

D

G

K

R

V

T

K

T

T

V

V

R

G

A

F

V

V

N

A

R

G

V

L

D

G

G

G

Q

E

I

V

T

T

P

H

S

D

E

E

I

F

L

Y

K

R

A

M

I

F

M

Q

Q

K

V

L

active site: R30 (≠ T38), E58 (= E65), R108 (≠ V131)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y27 (= Y35), P28 (= P36), I29 (= I37), E58 (= E65), V82 (≠ P89), R108 (≠ V131), F116 (≠ H139)

5exdD Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
24% identity, 96% coverage: 16:383/383 of query aligns to 8:374/394 of 5exdD

query
sites
5exdD

G

G

N

C

E

V

A

A

V

V

V

A

E

H

G

G

A

V

L

R

L

L

A

A

G

D

C

V

S

D

F

V

Y

I

G

C

G

S

Y
|
Y

P
|
P

I
|
I

T
x
R

P

P

S

Y

S

T

E

G

I

I

M

M

E

S

G

E

M

L

A

A

Q

R

R

M

L

V

P

A

K

D

T

G

E

E

-

L

T

D

G

A

T

E

F

F

L

V

Q

H

M

G

E

E

D

G

E
|
E

I

H

A

A

S

Q

M

L

G

S

A

V

V

V

I

Y

G

G

A

A

S

S

L

A

A

A

G

G

C

A

K

R

A

V

M

F

T

T

A

G

T

S

S

S

G

G

P

V

G

G

F

V

S

T

L

-

M

-

Q

-

E

-

H

-

L

-

G

-

Y

Y

A

A

C

M

M

E

V

V

E

Y

A

S

P

P

L

I

V

-

L

-

V

-

N

-

V

-

M

-

R

-

G

-

P

S

S

G

T

E

G

R

L

L

P

P

T

V

S

Q

P

M

A

A

Q

I

G

A

D

D

V
x
R

Q

T

Q

L

A

D

R

P

W

P

G

G

T
x
D

H

F

G

G

-

E

-

H

-

T

-

D

-

A

-

E

-

C

-

R

D

D

H

Q

A

G

I

W

L

I

V

Q

L

G

S

W

A

A

S

S

N

T

V

P

Q

Q

E

E

C

A

L

L

E

D

M

N

T

T

I

L

T

I

A

Y

F

Y

N

R

L

V

A

G

E

E

K

D

F

Q

R

R

T

V

-

L

P

P

V

Q

I

Y

L

A

L

C

L

L

D

D

-

G

E

Y

I

F

T

V

A

S

H

H

T

I

R

L

E

G

K

P

I

V

V

D

I

I

P

P

R

D

E

E

D

A

E

Q

Y

V

E

K

V

E

F

F

-

L

-

P

-

Y

-

K

-

N

N

H

R

H

V

V

V

L

P

D

T

P

M

R

P

K

P

P

E

Q

W

I

Y

I

K

G

P

P

F

Q

E

I

E

E

T

-

V

-

R

-

G

-

V

-

P

P

P

A

M

M

P

G

P

P

I

P

G

L

S

Q

G

Y

Y

Q

R

R

F

Y

H

Q

V

A

T

V

G

K

L

G

T

V

H

H

D

-

P

-

N

-

G

-

F

-

P

-

T

-

S

K

R

V

P

L

E

E

V

A

A

C

D

D

L

E

M

F

D

A

R

R

M

I

F

F

-

G

R

R

K

K

V

Y

D

D

Q

P

F

Y

L

L

P

-

Q

-

V

-

S

-

L

-

H

-

E

D

E

E

F

Y

R

L

C

T

E

D

D

D

A

A

E

E

V

V

C

I

V

I

V

F

A

G

Y

Q

G

G

S

A

V

H

A

M

R

E

S

T

A

A

E

K

Y

A

A

V

V

A

E

R

Q

R

A

L

R

R

E

N

R

L

G

G

V

E

K

K

A

V

G

G

L

V

L

A

K

R

L

L

K

R

T

T

L

F

Y

R

P

P

Y

F

P

P

R

T

A

E

A

Q

T

I

E

K

K

E

V

R

M

L

G

S

H

K

A

F

R

K

A

A

L

I

I

G

V

V

P

L

E

D

M

V

N

S

-

A

-

N

-

F

-

G

-

I

-

S

-

C

-

S

-

G

-

V

-

L

M

L

G

S

Q

E

I

L

S

R

R

A

E

A

V

L

K

Y

R

D

V

Y

N

G

N

D

G

K

R

V

T

K

T

T

V

V

R

G

A

F

V

V

N

A

R

G

V

L

D

G

G

G

Q

E

I

V

T

T

P

H

S

D

E

E

I

F

L

Y

K

R

A

M

I

F

M

Q

Q

K

V

L

active site: R30 (≠ T38), E58 (= E65), R108 (≠ V131)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y27 (= Y35), P28 (= P36), I29 (= I37), R30 (≠ T38), E58 (= E65), R108 (≠ V131), D115 (≠ T138)

5c4iA Structure of an oxalate oxidoreductase (see paper)
24% identity, 96% coverage: 16:383/383 of query aligns to 8:374/394 of 5c4iA

query
sites
5c4iA

G

G

N

C

E

V

A

A

V

V

V

A

E

H

G

G

A

V

L

R

L

L

A

A

G

D

C

V

S

D

F

V

Y

I

G

C

G

S

Y
|
Y

P

P

I
|
I

T
x
R

P

P

S

Y

S

T

E

G

I

I

M

M

E

S

G

E

M

L

A

A

Q

R

R

M

L

V

P

A

K

D

T

G

E

E

-

L

T

D

G

A

T

E

F

F

L

V

Q

H

M

G

E

E

D

G

E
|
E

I

H

A

A

S

Q

M

L

G

S

A

V

V

V

I

Y

G

G

A

A

S

S

L

A

A

A

G

G

C

A

K

R

A

V

M

F

T

T

A

G

T

S

S

S

G

G

P
x
V

G

G

F

V

S

T

L

-

M

-

Q

-

E

-

H

-

L

-

G

-

Y

Y

A

A

C

M

M

E

V

V

E

Y

A

S

P

P

L

I

V

-

L

-

V

-

N

-

V

-

M

-

R

-

G

-

P

S

S

G

T

E

G

R

L

L

P

P

T

V

S

Q

P

M

A

A

Q

I

G

A

D

D

V
x
R

Q

T

Q

L

A

D

R

P

W

P

G

G

T

D

H

F

G

G

-

E

-

H

-

T

-

D

-

A

-

E

-

C

-

R

D

D

H

Q

A

G

I

W

L

I

V

Q

L

G

S

W

A

A

S

S

N

T

V

P

Q

Q

E

E

C

A

L

L

E

D

M

N

T

T

I

L

T

I

A

Y

F

Y

N

R

L

V

A

G

E

E

K

D

F

Q

R

R

T

V

-

L

P

P

V

Q

I

Y

L

A

L

C

L

L

D

D

-

G

E

Y

I

F

T

V

A

S

H

H

T

I

R

L

E

G

K

P

I

V

V

D

I

I

P

P

R

D

E

E

D

A

E

Q

Y

V

E

K

V

E

F

F

-

L

-

P

-

Y

-

K

-

N

N

H

R

H

V

V

V

L

P

D

T

P

M

R

P

K

P

P

E

Q

W

I

Y

I

K

G

P

P

F

Q

E

I

E

E

T

-

V

-

R

-

G

-

V

-

P

P

P

A

M

M

P

G

P

P

I

P

G

L

S

Q

G

Y

Y

Q

R

R

F

Y

H

Q

V

A

T

V

G

K

L

G

T

V

H

H

D

-

P

-

N

-

G

-

F

-

P

-

T

-

S

K

R

V

P

L

E

E

V

A

A

C

D

D

L

E

M

F

D

A

R

R

M

I

F

F

-

G

R

R

K

K

V

Y

D

D

Q

P

F

Y

L

L

P

-

Q

-

V

-

S

-

L

-

H

-

E

D

E

E

F

Y

R

L

C

T

E

D

D

D

A

A

E

E

V

V

C

I

V

I

V

F

A

G

Y

Q

G

G

S

A

V

H

A

M

R

E

S

T

A

A

E

K

Y

A

A

V

V

A

E

R

Q

R

A

L

R

R

E

N

R

L

G

G

V

E

K

K

A

V

G

G

L

V

L

A

K

R

L

L

K

R

T

T

L

F

Y

R

P

P

Y

F

P

P

R

T

A

E

A

Q

T

I

E

K

K

E

V

R

M

L

G

S

H

K

A

F

R

K

A

A

L

I

I

G

V

V

P

L

E

D

M

V

N

S

-

A

-

N

-

F

-

G

-

I

-

S

-

C

-

S

-

G

-

V

-

L

M

L

G

S

Q

E

I

L

S

R

R

A

E

A

V

L

K

Y

R

D

V

Y

N

G

N

D

G

K

R

V

T

K

T

T

V

V

R

G

A

F

V

V

N

A

R

G

V

L

D

G

G

G

Q

E

I

V

T

T

P

H

S

D

E

E

I

F

L

Y

K

R

A

M

I

F

M

Q

Q

K

V

L

active site: R30 (≠ T38), E58 (= E65), R108 (≠ V131)
binding thiamine diphosphate: Y27 (= Y35), I29 (= I37), E58 (= E65), V82 (≠ P89)

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 77% coverage: 35:330/383 of query aligns to 25:314/1165 of 6cipA

query
sites
6cipA

Y

Y

P

P

I
|
I

T
|
T

P

P

S

S

-

P

-

M

-

A

E

E

I

I

M

A

E

D

G

E

M

W

A

A

Q

A

R

H

L

G

P

R

K

K

T

N

E

I

T

F

G

G

T

K

F

T

L

L

Q

Q

-

V

-

A

-

E

M

M

E

Q

D

S

E
|
E

I

A

A

G

S

A

M

A

G

G

A

A

V

V

I

H

G

G

A

S

S

L

L

A

A

A

G

G

C

A

K

L

A

T

M

T

T

T

A

F

T

T

S

A

G

S

P
x
Q

G

G

F

L

S

L

L

L

M

M

Q

I

E

P

H

N

L

M

G

Y

Y

K

A

I

C

A

M

G

V

E

E

L

A

L

P

P

L

C

V

V

L

F

V

H

N

V

V

A

M

A

R
|
R

G

A

G

L

P

S

S

T

T

H

G

A

L

L

P

S

T

I

S

F

P

G

A

D

Q

H

G

A

D

D

V

V

Q

M

Q

A

A

A

R

R

W

-

G

-

T

-

H

-

G

-

D

Q

H

T

A

G

I

F

L

A

V

M

L

L

S

S

A

S

S

A

N

S

V

V

Q

Q

E

E

C

V

L

M

E

D

M

L

T

A

I

L

T

V

A

A

F

H

N

L

L

A

A

T

E

L

K

K

F

A

R

R

T

V

P

P

V

F

I

V

L

H

L

F

D

D

E

G

I

F

-

R

T

T

A

S

H

H

T

E

R

V

E

Q

K

K

I

I

V

D

I

V

P

I

R

-

E

-

D

-

E

E

Y

Y

E

E

V

D

F

M

N

A

R

K

V

L

V

V

P

-

T

-

M

-

P

-

E

D

W

W

-

D

-

A

Y

I

K

R

P

A

F

F

E

R

E

Q

T

-

V

-

R

R

G

A

V

L

P

N

P

P

M

E

P

H

P

P

I

-

G

-

S

-

G

-

Y

-

R

-

F

-

H

H

V

Q

T

R

G

G

L

T

T

A

H

Q

D

N

P

P

N

D

G

I

F

Y

P

F

T

Q

S

S

R

R

-

E

-

A

-

N

-

P

-

Y

-

L

-

A

-

T

P

P

E

G

E

I

V

V

A

A

D

Q

L

V

M

M

D

E

R

Q

M

V

F

A

R

G

K

L

V

T

D

G

Q

R

F

-

L

-

P

-

Q

H

V

Y

S

H

L

L

H

F

E

D

E

Y

F

A

R

G

C

A

E

P

D

D

A

A

E

E

V

R

C

V

V

I

V

V

A

S

Y

M

G

G

S

S

V

S

A

C

R

E

S

V

A

I

E

E

Y

E

A

T

V

V

E

N

Q

Y

A

L

R

V

E

E

R

K

G

G

V

E

K

K

A

V

G

G

L

L

L

I

K

K

L

V

K

R

T

L

L

F

Y

R

P

P

Y

F

P

S

R

A

A

E

A

H

T

F

E

L

K

K

V

V

M

L

active site: T28 (= T38), E61 (= E65), R111 (= R115)
binding 2-acetyl-thiamine diphosphate: I27 (= I37), E61 (= E65), Q85 (≠ P89), R111 (= R115)

Sites not aligning to the query:

active site: 999
binding 2-acetyl-thiamine diphosphate: 813, 834, 835, 836, 865, 965, 966, 967, 996, 998, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 998, 1074

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 77% coverage: 35:330/383 of query aligns to 26:315/1171 of Q2RMD6

query
sites
Q2RMD6

Y

Y

P

P

I

I

T

T

P

P

S

S

-

P

-

M

-

A

E

E

I

I

M

A

E

D

G

E

M

W

A

A

Q

A

R

H

L

G

P

R

K

K

T

N

E

I

T

F

G

G

T

K

F

T

L

L

Q

Q

-

V

-

A

-

E

M

M

E

Q

D

S

E

E

I

A

A

G

S

A

M

A

G

G

A

A

V

V

I

H

G

G

A

S

S

L

L

A

A

A

G

G

C

A

K

L

A

T

M

T

T

T

A

F

T

T

S

A

G

S

P

Q

G

G

F

L

S

L

L

L

M

M

Q

I

E

P

H

N

L

M

G

Y

Y

K

A

I

C

A

M

G

V

E

E

L

A

L

P

P

L

C

V

V

L

F

V

H

N

V

V

A

M

A

R

R

G

A

G

L

P

S

S

T

T

H

G

A

L

L

P

S

T

I

S

F

P

G

A

D

Q

H

G

A

D

D

V

V

Q

M

Q

A

A

A

R

R

W

-

G

-

T

-

H

-

G

-

D

Q

H

T

A

G

I

F

L

A

V

M

L

L

S

S

A

S

S

A

N

S

V

V

Q

Q

E

E

C

V

L

M

E

D

M

L

T

A

I

L

T

V

A

A

F

H

N

L

L

A

A

T

E

L

K

K

F

A

R

R

T

V

P

P

V

F

I

V

L

H

L

F

D

D

E

G

I

F

-

R

T

T

A

S

H

H

T

E

R

V

E

Q

K

K

I

I

V

D

I

V

P

I

R

-

E

-

D

-

E

E

Y

Y

E

E

V

D

F

M

N

A

R

K

V

L

V

V

P

-

T

-

M

-

P

-

E

D

W

W

Y

D

K

A

P

I

F

R

E

A

E

F

T

R

V

Q

R

R

G

A

V

L

P

N

P

P

M

E

P

H

P

P

I

-

G

-

S

-

G

-

Y

-

R

-

F

-

H

H

V

Q

T

R

G

G

L

T

T

A

H

Q

D

N

P

P

N

D

G

I

F

Y

P

F

T

Q

S

S

R

R

-

E

-

A

-

N

-

P

-

Y

-

L

-

A

-

T

P

P

E

G

E

I

V

V

A

A

D

Q

L

V

M

M

D

E

R

Q

M

V

F

A

R

G

K

L

V

T

D

G

Q

R

F

-

L

-

P

-

Q

H

V

Y

S

H

L

L

H

F

E

D

E

Y

F

A

R

G

C

A

E

P

D

D

A

A

E

E

V

R

C

V

V

I

V

V

A

S

Y

M

G

G

S

S

V

S

A

C

R

E

S

V

A

I

E

E

Y

E

A

T

V

V

E

N

Q

Y

A

L

R

V

E

E

R

K

G

G

V

E

K

K

A

V

G

G

L

L

L

I

K

K

L

V

K

R

T

L

L

F

Y

R

P

P

Y

F

P

S

R

A

A

E

A

H

T

F

E

L

K

K

V

V

M

L

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate
967 binding Mg(2+)
967:969 binding thiamine diphosphate
995 binding Mg(2+)
995:1000 binding thiamine diphosphate
997 binding Mg(2+)
1075 binding [4Fe-4S] cluster

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 77% coverage: 35:330/383 of query aligns to 25:314/1164 of 6cioA

query
sites
6cioA

Y

Y

P
|
P

I
|
I

T
|
T

P

P

S

S

-

P

-

M

-

A

E

E

I

I

M

A

E

D

G

E

M

W

A

A

Q

A

R

H

L

G

P

R

K

K

T

N

E

I

T

F

G

G

T

K

F

T

L

L

Q

Q

-

V

-

A

-

E

M

M

E

Q

D

S

E
|
E

I

A

A

G

S

A

M

A

G

G

A

A

V

V

I

H

G

G

A

S

S

L

L

A

A

A

G

G

C

A

K

L

A

T

M

T

T

T

A

F

T

T

S

A

G

S

P
x
Q

G

G

F

L

S

L

L

L

M

M

Q

I

E

P

H

N

L

M

G

Y

Y

K

A

I

C

A

M

G

V

E

E

L

A

L

P

P

L

C

V

V

L

F

V

H

N

V

V

A

M

A

R
|
R

G

A

G

L

P

S

S

T

T

H

G

A

L

L

P

S

T

I

S

F

P

G

A

D

Q

H

G

A

D

D

V

V

Q

M

Q

A

A

A

R

R

W

-

G

-

T

-

H

-

G

-

D

Q

H

T

A

G

I

F

L

A

V

M

L

L

S

S

A

S

S

A

N

S

V

V

Q

Q

E

E

C

V

L

M

E

D

M

L

T

A

I

L

T

V

A

A

F

H

N

L

L

A

A

T

E

L

K

K

F

A

R

R

T

V

P

P

V

F

I

V

L

H

L

F

D

D

E

G

I

F

-

R

T

T

A

S

H

H

T

E

R

V

E

Q

K

K

I

I

V

D

I

V

P

I

R

-

E

-

D

-

E

E

Y

Y

E

E

V

D

F

M

N

A

R

K

V

L

V

V

P

-

T

-

M

-

P

-

E

D

W

W

-

D

-

A

Y

I

K

R

P

A

F

F

E

R

E

Q

T

-

V

-

R

R

G

A

V

L

P

N

P

P

M

E

P

H

P

P

I

-

G

-

S

-

G

-

Y

-

R

-

F

-

H

H

V

Q

T

R

G

G

L

T

T

A

H

Q

D

N

P

P

N

D

G

I

F

Y

P

F

T

Q

S

S

R

R

-

E

-

A

-

N

-

P

-

Y

-

L

-

A

-

T

P

P

E

G

E

I

V

V

A

A

D

Q

L

V

M

M

D

E

R

Q

M

V

F

A

R

G

K

L

V

T

D

G

Q

R

F

-

L

-

P

-

Q

H

V

Y

S

H

L

L

H

F

E

D

E

Y

F

A

R

G

C

A

E

P

D

D

A

A

E

E

V

R

C

V

V

I

V

V

A

S

Y

M

G

G

S

S

V

S

A

C

R

E

S

V

A

I

E

E

Y

E

A

T

V

V

E

N

Q

Y

A

L

R

V

E

E

R

K

G

G

V

E

K

K

A

V

G

G

L

L

L

I

K

K

L

V

K

R

T

L

L

F

Y

R

P

P

Y

F

P

S

R

A

A

E

A

H

T

F

E

L

K

K

V

V

M

L

active site: T28 (= T38), E61 (= E65), R111 (= R115)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: P26 (= P36), I27 (= I37), T28 (= T38), E61 (= E65), Q85 (≠ P89), R111 (= R115)

Sites not aligning to the query:

active site: 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 1074, 1075
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 813, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 77% coverage: 35:330/383 of query aligns to 25:314/1169 of 6ciqA

query
sites
6ciqA

Y

Y

P
|
P

I
|
I

T
|
T

P

P

S

S

-

P

-

M

-

A

E

E

I

I

M

A

E

D

G

E

M

W

A

A

Q

A

R

H

L

G

P

R

K

K

T

N

E

I

T

F

G

G

T

K

F

T

L

L

Q

Q

-

V

-

A

-

E

M

M

E

Q

D

S

E
|
E

I

A

A

G

S

A

M

A

G

G

A

A

V

V

I

H

G

G

A

S

S

L

L

A

A

A

G

G

C

A

K

L

A

T

M

T

T

T

A

F

T

T

S

A

G

S

P
x
Q

G

G

F

L

S

L

L

L

M

M

Q

I

E

P

H

N

L

M

G

Y

Y

K

A

I

C

A

M

G

V

E

E

L

A

L

P

P

L

C

V

V

L

F

V

H

N

V

V

A

M

A

R
|
R

G

A

G

L

P

S

S

T

T

H

G

A

L

L

P

S

T

I

S

F

P

G

A

D

Q

H

G

A

D

D

V

V

Q

M

Q

A

A

A

R

R

W

-

G

-

T

-

H

-

G

-

D

Q

H

T

A

G

I

F

L

A

V

M

L

L

S

S

A

S

S

A

N

S

V

V

Q

Q

E

E

C

V

L

M

E

D

M

L

T

A

I

L

T

V

A

A

F

H

N

L

L

A

A

T

E

L

K

K

F

A

R

R

T

V

P

P

V

F

I

V

L

H

L

F

D

D

E

G

I

F

-

R

T

T

A

S

H

H

T

E

R

V

E

Q

K

K

I

I

V

D

I

V

P

I

R

-

E

-

D

-

E

E

Y

Y

E

E

V

D

F

M

N

A

R

K

V

L

V

V

P

-

T

-

M

-

P

-

E

D

W

W

Y

D

K

A

P

I

F

R

E

A

E

F

T

R

V

Q

R

R

G

A

V

L

P

N

P

P

M

E

P

H

P

P

I

-

G

-

S

-

G

-

Y

-

R

-

F

-

H

H

V

Q

T

R

G

G

L

T

T

A

H

Q

D

N

P

P

N

D

G

I

F

Y

P

F

T

Q

S

S

R

R

-

E

-

A

-

N

-

P

-

Y

-

L

-

A

-

T

P

P

E

G

E

I

V

V

A

A

D

Q

L

V

M

M

D

E

R

Q

M

V

F

A

R

G

K

L

V

T

D

G

Q

R

F

-

L

-

P

-

Q

H

V

Y

S

H

L

L

H

F

E

D

E

Y

F

A

R

G

C

A

E

P

D

D

A

A

E

E

V

R

C

V

V

I

V

V

A

S

Y

M

G

G

S

S

V

S

A

C

R

E

S

V

A

I

E

E

Y

E

A

T

V

V

E

N

Q

Y

A

L

R

V

E

E

R

K

G

G

V

E

K

K

A

V

G

G

L

L

L

I

K

K

L

V

K

R

T

L

L

F

Y

R

P

P

Y

F

P

S

R

A

A

E

A

H

T

F

E

L

K

K

V

V

M

L

active site: T28 (= T38), E61 (= E65), R111 (= R115)
binding coenzyme a: T28 (= T38)
binding thiamine diphosphate: P26 (= P36), I27 (= I37), E61 (= E65), Q85 (≠ P89)

Sites not aligning to the query:

active site: 999
binding coenzyme a: 423, 424, 427, 428, 553, 586, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 813, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 77% coverage: 35:330/383 of query aligns to 25:314/1169 of 6cinB

query
sites
6cinB

Y

Y

P
|
P

I
|
I

T
|
T

P

P

S

S

-

P

-

M

-

A

E

E

I

I

M

A

E

D

G

E

M

W

A

A

Q

A

R

H

L

G

P

R

K

K

T

N

E

I

T

F

G

G

T

K

F

T

L

L

Q

Q

-

V

-

A

-

E

M

M

E

Q

D

S

E
|
E

I

A

A

G

S

A

M

A

G

G

A

A

V

V

I

H

G

G

A

S

S

L

L

A

A

A

G

G

C

A

K

L

A

T

M

T

T

T

A

F

T

T

S

A

G

S

P

Q

G

G

F

L

S

L

L

L

M

M

Q

I

E

P

H

N

L

M

G

Y

Y

K

A

I

C

A

M

G

V

E

E

L

A

L

P

P

L

C

V

V

L

F

V

H

N

V

V

A

M

A

R
|
R

G

A

G

L

P

S

S

T

T

H

G

A

L

L

P

S

T

I

S

F

P

G

A

D

Q

H

G

A

D

D

V

V

Q

M

Q

A

A

A

R

R

W

-

G

-

T

-

H

-

G

-

D

Q

H

T

A

G

I

F

L

A

V

M

L

L

S

S

A

S

S

A

N

S

V

V

Q

Q

E

E

C

V

L

M

E

D

M

L

T

A

I

L

T

V

A

A

F

H

N

L

L

A

A

T

E

L

K

K

F

A

R

R

T

V

P

P

V

F

I

V

L

H

L

F

D

D

E

G

I

F

-

R

T

T

A

S

H

H

T

E

R

V

E

Q

K

K

I

I

V

D

I

V

P

I

R

-

E

-

D

-

E

E

Y

Y

E

E

V

D

F

M

N

A

R

K

V

L

V

V

P

-

T

-

M

-

P

-

E

D

W

W

Y

D

K

A

P

I

F

R

E

A

E

F

T

R

V

Q

R

R

G

A

V

L

P

N

P

P

M

E

P

H

P

P

I

-

G

-

S

-

G

-

Y

-

R

-

F

-

H

H

V

Q

T

R

G

G

L

T

T

A

H

Q

D

N

P

P

N

D

G

I

F

Y

P

F

T

Q

S

S

R

R

-

E

-

A

-

N

-

P

-

Y

-

L

-

A

-

T

P

P

E

G

E

I

V

V

A

A

D

Q

L

V

M

M

D

E

R

Q

M

V

F

A

R

G

K

L

V

T

D

G

Q

R

F

-

L

-

P

-

Q

H

V

Y

S

H

L

L

H

F

E

D

E

Y

F

A

R

G

C

A

E

P

D

D

A

A

E

E

V

R

C

V

V

I

V

V

A

S

Y

M

G

G

S

S

V

S

A

C

R

E

S

V

A

I

E

E

Y

E

A

T

V

V

E

N

Q

Y

A

L

R

V

E

E

R

K

G

G

V

E

K

K

A

V

G

G

L

L

L

I

K

K

L

V

K

R

T

L

L

F

Y

R

P

P

Y

F

P

S

R

A

A

E

A

H

T

F

E

L

K

K

V

V

M

L

active site: T28 (= T38), E61 (= E65), R111 (= R115)
binding thiamine diphosphate: P26 (= P36), I27 (= I37), E61 (= E65)

Sites not aligning to the query:

active site: 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

Query Sequence

>WP_078716536.1 NCBI__GCF_900167125.1:WP_078716536.1
MAAGRKKKRKELFAQGNEAVVEGALLAGCSFYGGYPITPSSEIMEGMAQRLPKTETGTFL
QMEDEIASMGAVIGASLAGCKAMTATSGPGFSLMQEHLGYACMVEAPLVLVNVMRGGPST
GLPTSPAQGDVQQARWGTHGDHAILVLSASNVQECLEMTITAFNLAEKFRTPVILLLDEI
TAHTREKIVIPREDEYEVFNRVVPTMPPEWYKPFEETVRGVPPMPPIGSGYRFHVTGLTH
DPNGFPTSRPEEVADLMDRMFRKVDQFLPQVSLHEEFRCEDAEVCVVAYGSVARSAEYAV
EQARERGVKAGLLKLKTLYPYPRAATEKVMGHARALIVPEMNMGQISREVKRVNNGRTTV
RAVNRVDGQIITPSEILKAIMQV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory