SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_078717333.1 NCBI__GCF_900167125.1:WP_078717333.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
48% identity, 88% coverage: 1:152/172 of query aligns to 1:152/173 of P10880

query
sites
P10880

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C13 (≠ S13) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K15) mutation to M: Loss of activity. Increased thermostability.
D34 (= D34) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
47% identity, 88% coverage: 1:152/172 of query aligns to 1:152/170 of 1e6cA

query
sites
1e6cA

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binding phosphate ion: R11 (= R11), G12 (≠ A12), M15 (≠ K15), T16 (= T16)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
46% identity, 88% coverage: 1:152/172 of query aligns to 1:142/162 of 2shkB

query
sites
2shkB

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binding adenosine-5'-diphosphate: G12 (≠ A12), C13 (≠ S13), G14 (= G14), K15 (= K15), T16 (= T16), T17 (= T17), R110 (= R110)
binding magnesium ion: Q134 (≠ C144), A137 (= A147)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 145, 147

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
43% identity, 88% coverage: 1:152/172 of query aligns to 1:137/158 of 1shkA

query
sites
1shkA

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binding magnesium ion: R92 (≠ Q93), A93 (vs. gap), G95 (= G95), Q129 (≠ C144), A132 (= A147)

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
32% identity, 97% coverage: 3:168/172 of query aligns to 2:162/168 of 3n2eA

query
sites
3n2eA

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A

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ A10), R45 (≠ E46), F48 (≠ V49), R57 (= R58), G80 (= G80), G81 (= G81), M84 (≠ L84), R116 (= R120), R132 (= R139), L135 (= L142), Y136 (= Y143)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
32% identity, 96% coverage: 3:167/172 of query aligns to 1:160/160 of 3mufA

query
sites
3mufA

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binding adenosine-5'-diphosphate: G10 (≠ A12), S11 (= S13), G12 (= G14), K13 (= K15), S14 (≠ T16), S15 (≠ T17), M98 (≠ V102), R106 (= R110), G147 (= G154), G148 (≠ T155)
binding shikimate-3-phosphate: M9 (≠ R11), D32 (= D34), R56 (= R58), G78 (= G79), G79 (= G80), G80 (= G81), R115 (= R120), R131 (= R139)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
32% identity, 98% coverage: 4:171/172 of query aligns to 10:178/179 of 4y0aA

query
sites
4y0aA

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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R11), D40 (= D34), F63 (= F57), R64 (= R58), G86 (= G80), G87 (= G81), L126 (≠ S122), R143 (= R139)
binding sulfate ion: G18 (≠ A12), A19 (≠ S13), G20 (= G14), K21 (= K15), T22 (= T16), E112 (= E105), R124 (= R120), R137 (= R133)

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
32% identity, 96% coverage: 3:167/172 of query aligns to 1:158/158 of 1zuiA

query
sites
1zuiA

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A

G

R

L

E

A

L

L

N

K

E

L

G

E

F

V

T

L

D

D

T

T

D
|
D

A

M

I

I

V

I

V

S

E

E

R

R

I

V

G

G

M

L

V

S

V

V

E

R

K

E

Y

I

V
x
F

E

E

Q

E

N

L

G

G

W

E

A

D

A

N

F

F

R
|
R

R

M

A

F

E

E

S

K

Q

N

A

L

L

I

R

D

D

E

A

L

A

K

D

T

-

L

Q

K

P

T

P

P

H

H

I

V

V

I

A

S

C

T

G
|
G

G

G

I

I

V

V

L

M

S

H

R

E

T

N

N

L

R

K

D

G

I

L

L

-

Q

-

G

G

V

T

V

F

Y

Y

L

L

R

K

V

M

P

D

E

F

E

E

E

T

L

L

I

I

R

K

R

R

L

L

E

N

N

Q

D

R

P
x
E

K

K

S

-

G

-

Q

-

R
|
R

P

P

S

L

L

L

T

N

G

N

R

-

G

-

L

-

V

L

E

T

E

Q

V

A

R

K

E

E

V

L

L

F

G

E

E

K

R
|
R

E

Q

P

A

L

L

Y

Y

C

E

S

K

C

N

A

A

S

S

L

F

T

I

V

I

E

D

-

A

H

R

G

G

T

G

L

L

A

N

Q

N

Q

S

V

L

R

K

V

Q

V

V

Q

L

D

Q

F

F

L

I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R11), D32 (= D34), F47 (≠ V49), R56 (= R58), G78 (= G79), G79 (= G80), E111 (≠ P115), R113 (= R120), R129 (= R139)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
35% identity, 85% coverage: 7:152/172 of query aligns to 6:146/163 of 1u8aA

query
sites
1u8aA

L

L

I

V

G

G

A

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V
x
F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R
|
R

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

G
|
G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

-

E

-

N

-

D

-

P

-

K

T

S

G

G

G

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-diphosphate: G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), F48 (≠ V49), R57 (= R58), G79 (= G80), G80 (= G81), L116 (≠ S122), R133 (= R139)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/165 of 3bafA

query
sites
3bafA

L

L

I

V

G

G

A

L

R
x
P

A

G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R11), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R135 (= R139)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/165 of 2dfnA

query
sites
2dfnA

L

L

I

V

G

G

A

L

R
x
P

A
x
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V
x
I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F
|
F

R
|
R

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G
|
G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-diphosphate: G11 (≠ A12), S12 (= S13), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding chloride ion: P10 (≠ R11), K14 (= K15), G79 (= G80)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), I44 (≠ V45), F56 (= F57), R57 (= R58), G78 (= G79), G79 (= G80), G80 (= G81), R135 (= R139)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/165 of 1we2A

query
sites
1we2A

L

L

I

V

G

G

A

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V
x
I

E

A

K

D

Y

I

V
x
F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G
|
G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R
|
R

P

P

S
x
L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding 3-dehydroshikimate: D33 (= D34), I44 (≠ V45), F48 (≠ V49), G78 (= G79), G79 (= G80), G80 (= G81), L118 (≠ S122), R135 (= R139)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/179 of 2iyvA

query
sites
2iyvA

L

L

I

V

G

G

A

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D

D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R

R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-diphosphate: G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/178 of 2iywA

query
sites
2iywA

L

L

I

V

G

G

A

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D

D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R

R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-triphosphate: G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/169 of 4bqsA

query
sites
4bqsA

L

L

I

V

G

G

A

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D34), R135 (= R139)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/169 of 2iyyA

query
sites
2iyyA

L

L

I

V

G

G

A

L

R

P

A

G

S

S

G

G

K
|
K

T

S

T

T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V
x
E

E

Q

R

R

I

T

G

G

M

R

V
x
S

V
x
I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R
|
R

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

G
|
G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding magnesium ion: E37 (≠ V38), S43 (≠ V44), I44 (≠ V45)
binding phosphate ion: K14 (= K15), G79 (= G80)
binding shikimate-3-phosphate: K14 (= K15), D33 (= D34), R57 (= R58), G79 (= G80), G80 (= G81), L118 (≠ S122), R135 (= R139)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
34% identity, 85% coverage: 7:152/172 of query aligns to 6:148/168 of 1zyuA

query
sites
1zyuA

L

L

I

V

G

G

A

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

I

G

G

R

R

E

R

L

L

A

A

R

K

E

A

L

L

N

G

E

V

G

G

F

L

T

L

D

D

T

T

D
|
D

A

V

I

A

V

I

V

E

E

Q

R

R

I

T

G

G

M

R

V

S

V

I

E

A

K

D

Y

I

V

F

E

A

Q

T

N

D

G

G

W

E

A

Q

A

E

F

F

R
|
R

R

R

A

I

E

E

S

E

Q

D

A

V

L

V

R

R

D

A

A

A

A

L

D

A

Q

D

P

H

P

D

H

G

I

V

V

L

A

S

C

L

G

G

G
|
G

I

A

V

V

L

T

S

S

R

P

T

G

N

V

R

R

D

A

I

A

L

L

Q

A

Q

G

G

H

V

T

V

V

Y

Y

L

L

R

E

V

I

P

S

E

A

E

E

L

G

I

V

R

R

R
|
R

L

T

E

G

N

G

D

N

P

T

K

-

S

-

G

-

Q

V

R

R

P
|
P

S
x
L

L

L

T

A

G

G

R

P

G

D

L

R

V

A

E

E

E

K

V

Y

R

R

E

A

V

L

L

M

G

A

E

K

R
|
R

E

A

P

P

L

L

Y

Y

C

R

S

R

C

V

A

A

S

T

L

M

T

R

V

V

E

D

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R11), G11 (≠ A12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R57 (= R58), G80 (= G81), P117 (= P121), L118 (≠ S122), R135 (= R139)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 149, 152, 154

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
29% identity, 86% coverage: 2:149/172 of query aligns to 842:989/1555 of 6hqvA

query
sites
6hqvA

E

D

Q

R

H

S

V

I

F

F

L

I

I

V

G

G

A

M

R

R

A

G

S

A

G

G

K

K

T

S

T

T

L

A

G

G

R

R

E

W

L

M

A

S

R

E

E

L

L

L

N

K

E

R

G

P

F

L

T

V

D

D

T

L

D
|
D

A

A

I

E

V

L

V

E

E

R

R

R

I

E

G

G

M

M

V

T

V

I

E

P

K

E

Y

I

V

I

E

R

-

G

Q

E

N

R

G

G

W

W

A

E

A

G

F

F

R

R

R

Q

A

A

E

E

S

L

Q

E

A

L

L

L

R

Q

D

D

A

V

A

I

D

K

Q

N

P

Q

P

S

-

K

-

G

H

Y

I

I

V

F

A

S

C

C

G

G

G
|
G

I

I

V

V

L

E

S

T

R

E

T

A

N

A

R

R

D

K

I

L

L

L

-

I

-

D

-

Y

-

H

-

K

Q

N

Q

G

G

G

V

P

V

V

V

L

Y

L

L

V

R

H

V

R

P

D

E

T

E

D

E

Q

L

V

I

V

R

E

R

Y

L

L

E

M

N

R

D

D

P

-

K

-

S

-

G

K

Q

T

R

R

P

P

S

A

L

Y

T

S

G

-

R

-

G

-

L

-

V

-

E

E

E

N

V

I

R

R

E

E

V

V

L

Y

G

E

E

R

R
|
R

E

K

P

P

L

W

Y

F

C

Y

S

E

C

C

A

S

S

N

L

L

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D34), G923 (= G80), G924 (= G81), R979 (= R139)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
28% identity, 84% coverage: 5:149/172 of query aligns to 866:1009/1583 of P07547

query
sites
P07547

V

I

F

Y

L

I

I

I

G

G

A

M

R

R

A

G

S

A

G

G

K

K

T

S

T

T

L

A

G

G

R

N

E

W

L

V

A

S

R

K

E

A

L

L

N

N

E

R

G

P

F

F

T

V

D

D

T

L

D

D

A

T

I

E

V

L

V

E

E

T

R

V

I

E

G

G

M

M

V

T

V

I

E

P

K

D

Y

I

V

I

E

K

Q

T

N

R

G

G

W

W

A

Q

A

G

F

F

R

R

R

N

A

A

E

E

S

L

Q

E

A

I

L

L

R

K

D

R

A

T

A

L

D

K

Q

E

P

R

P

S

-

R

-

G

H

Y

I

V

V

F

A

A

C

C

G

G

I

V

V

V

L

E

S

M

R

P

T

E

N

A

R

R

D

K

I

L

L

L

-

T

-

D

-

Y

-

H

-

K

Q

T

Q

K

G

G

V

N

V

V

V

L

Y

L

L

L

R

M

V

R

P

D

E

I

E

K

L

I

I

M

R

D

R

F

L

L

E

S

N

I

D

D

P

-

K

-

S

-

G

K

Q

T

R

R

P

P

S

A

L

-

T

-

G

-

R

-

G

-

L

Y

V

V

E

E

E

D

V

M

R

M

E

G

V

V

L

W

G

L

E

R

R

R

E

K

P

P

L

W

Y

F

C

Q

S

E

C

C

A

S

S

N

L

I

Sites not aligning to the query:

44:46 binding NAD(+)
81:84 binding NAD(+)
114:116 binding NAD(+)
119 binding NAD(+)
139:140 binding NAD(+)
161 binding NAD(+)
179:182 binding NAD(+)
190 binding NAD(+)
194 binding Zn(2+)
271 binding Zn(2+)
287 binding Zn(2+)

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
25% identity, 85% coverage: 2:148/172 of query aligns to 3:134/682 of 7tbvA

query
sites
7tbvA

E

D

Q

K

H

S

V

I

F

I

L

V

I

I

G

G

A

M

R

R

A

G

S

T

G

G

K

K

T

S

T

T

L

L

G

S

R

E

E

W

L

L

A

A

R

S

E

F

L

L

N

G

E

F

G

K

F

M

T

L

D

D

T

M

D

D

A

K

I

Y

V

L

V

E

E

E

R

K

I

L

G

G

M

T

V

G

V

I

E

K

K

S

Y

L

V

I

E

K

Q

A

N

K

G

G

W

W

A

E

A

Y

F

F

R

R

R

Q

A

E

E

E

S

A

Q

I

A

V

L

A

R

K

D

E

A

C

A

F

D

T

Q

K

-

F

-

S

P

K

P

G

H

Y

I

V

V

L

A

S

C

T

G

G

I

I

V

V

L

E

S

G

R

E

T

D

N

A

R

R

D

Q

I

Q

L

L

Q

K

Q

S

-

Y

-

A

-

D

-

N

-

G

G

G

V

I

V

V

V

L

Y

H

L

L

R

H

V

-

P

-

E

-

L

-

I

-

R

R

R

D

L

L

E

D

N

E

D

S

P

-

K

-

S

-

G

-

Q

-

R

-

P

-

S

-

L

-

T

-

G

-

R

-

G

-

L

-

V

-

E

S

E

E

V

V

R

Q

E

E

V

V

L

W

G

L

E

R

R

R

E

E

P

K

L

W

Y

Y

C

H

S

E

C

C

A

S

S

N

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

Query Sequence

>WP_078717333.1 NCBI__GCF_900167125.1:WP_078717333.1
MEQHVFLIGARASGKTTLGRELARELNEGFTDTDAIVVERIGMVVEKYVEQNGWAAFRRA
ESQALRDAADQPPHIVACGGGIVLSRTNRDILQQGVVVYLRVPEEELIRRLENDPKSGQR
PSLTGRGLVEEVREVLGEREPLYCSCASLTVEHGTLAQQVRVVQDFLSSSMG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory