SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
44% identity, 70% coverage: 16:203/267 of query aligns to 11:200/304 of Q96XT4

query
sites
Q96XT4

W

W

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C

P

P

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G

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C

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S

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D

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G

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A

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I

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N

G

H

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F

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H

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G

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R

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N

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L

H

N

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N
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I
x
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x
L

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K

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G

Q

Q

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A

A

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G

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V

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C

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Y

C12 (= C17) binding [4Fe-4S] cluster
C15 (= C20) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 49:52) binding thiamine diphosphate
C46 (= C51) binding [4Fe-4S] cluster
D90 (= D93) binding Mg(2+)
GD 91:92 (= GD 94:95) binding thiamine diphosphate
N118 (= N121) binding Mg(2+)
V120 (≠ I123) binding Mg(2+)
GL 122:123 (≠ GM 125:126) binding thiamine diphosphate
C197 (= C200) binding [4Fe-4S] cluster

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
39% identity, 90% coverage: 16:254/267 of query aligns to 11:266/305 of Q96Y68

query
sites
Q96Y68

W

W

C
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C

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P

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G

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C

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N

G

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E

C12 (= C17) binding [4Fe-4S] cluster
C15 (= C20) binding [4Fe-4S] cluster
C46 (= C51) binding [4Fe-4S] cluster
K49 (≠ R54) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D93) binding Mg(2+)
GD 91:92 (= GD 94:95) binding thiamine diphosphate
N118 (= N121) binding Mg(2+)
V120 (≠ I123) binding Mg(2+)
GL 122:123 (≠ GM 125:126) binding thiamine diphosphate
K125 (≠ G128) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K176) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C200) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
39% identity, 90% coverage: 16:254/267 of query aligns to 11:266/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

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N

G

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N

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E

K49 (≠ R54) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (≠ M126) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
39% identity, 90% coverage: 16:254/267 of query aligns to 7:248/286 of 5b48B

query
sites
5b48B

W

W

C
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C

P

P

G

G

C
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C

G

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N

G

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N

G

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A

A

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R

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N

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D

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G

I
x
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Y
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Y

G
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G

M
x
L

T

T

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G

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Q

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R

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N

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C

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T
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T

Y

Y

F

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G

N

R

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K

I

N

N

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M

K

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G

W

A

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L

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W

A

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K

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N

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K

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E

binding magnesium ion: D84 (= D93), N112 (= N121), V114 (≠ I123)
binding iron/sulfur cluster: C8 (= C17), C11 (= C20), C42 (= C51), C183 (= C200), T185 (= T202)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I49), G41 (= G50), C42 (= C51), S43 (= S52), H59 (= H68), G85 (= G94), D86 (= D95), N112 (= N121), V114 (≠ I123), Y115 (= Y124), G116 (= G125), L117 (≠ M126)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
43% identity, 70% coverage: 16:203/267 of query aligns to 4:182/275 of 5b47B

query
sites
5b47B

W

W

C
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C

P

P

G

G

C
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C

G

G

H

D

G

F

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G

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I

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S

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A

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G

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K

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K

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L

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G

G

D
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D

G
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G

D
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D

A

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M

L

A

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I

G

G

G

M

N

G

H

H

F

F

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H

H

A

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C

G

R

R

N

N

I

I

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D

M

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A

A

V

I

L

V

V

L

H

N

N

N
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N

R

G

I
x
V

Y
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Y

G

G

M
x
L

T

-

G

-

Q

Q

Y

A

S

S

P

P

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T

S

L

G

H

R

R

G

G

V

-

L

-

A

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-

A

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Y

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N

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I

D

M

D

D

D

A

F

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D

N

A

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L

E

A

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A

A

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L

G

A

A

A

G

G

A

Y

T

T

F

F

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A

A

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R

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T

Y

A

A

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K

K

E

I

L

L

I

T

K

K

K

A

A

I

I

E

L

H

H

K

K

G

G

F

S

S

A

V

L

V

V

E

D

V

I

M

L

V

Q

Q

P

C
|
C

P

P

T

T

Y

Y

binding magnesium ion: D83 (= D93), N111 (= N121)
binding iron/sulfur cluster: C5 (= C17), C8 (= C20), C39 (= C51), C179 (= C200)
binding thiamine diphosphate: I37 (= I49), G38 (= G50), C39 (= C51), S40 (= S52), H58 (= H68), G84 (= G94), D85 (= D95), N111 (= N121), V113 (≠ I123), Y114 (= Y124), L116 (≠ M126)

6a50A Structure of benzoylformate decarboxylases in complex with cofactor tpp
31% identity, 50% coverage: 69:201/267 of query aligns to 404:526/527 of 6a50A

query
sites
6a50A

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
|
M

T
x
L

G

R

G

W

Q

F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

M

S

V

T

Q

V

C

S

P

P

binding magnesium ion: D428 (= D93), N455 (= N121), T457 (≠ I123)
binding thiamine diphosphate: G427 (= G92), D428 (= D93), G429 (= G94), S430 (≠ D95), Y433 (≠ H103), N455 (= N121), T457 (≠ I123), Y458 (= Y124), G459 (= G125), M460 (= M126), L461 (≠ T127)

Sites not aligning to the query:

binding magnesium ion: 117, 118, 120
binding thiamine diphosphate: 23, 24, 47, 70, 377, 378, 401, 403

3f6bX Crystal structure of benzoylformate decarboxylase in complex with the pyridyl inhibitor paa (see paper)
31% identity, 50% coverage: 69:201/267 of query aligns to 403:525/525 of 3f6bX

query
sites
3f6bX

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G

G

D
|
D

G

G

D

S

A

A

M

-

A

-

I

-

G

-

N

N

H

Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G

G

M
x
A

T
x
L

G

R

G

W

Q

F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

M

S

V

T

Q

V

C
x
S

P

P

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), S524 (≠ C200)

Sites not aligning to the query:

3fznA Intermediate analogue in benzoylformate decarboxylase (see paper)
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/523 of 3fznA

query
sites
3fznA

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G426 (= G92), D427 (= D93), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), N454 (= N121), T456 (≠ I123), Y457 (= Y124), G458 (= G125), A459 (≠ M126)
binding magnesium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)

Sites not aligning to the query:

active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280, 375, 400, 402
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 22, 23, 24, 25, 46, 69, 280, 376, 377, 400, 402
binding phosphate ion: 286, 288, 289, 290

1bfdA Benzoylformate decarboxylase from pseudomonas putida (see paper)
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/523 of 1bfdA

query
sites
1bfdA

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)
binding calcium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)
binding thiamine diphosphate: G426 (= G92), D427 (= D93), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), T456 (≠ I123), Y457 (= Y124), G458 (= G125), A459 (≠ M126), L460 (≠ T127)

Sites not aligning to the query:

active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280, 375, 400, 402
binding magnesium ion: 116, 117, 119
binding thiamine diphosphate: 376, 377, 402

1pi3A E28q mutant benzoylformate decarboxylase from pseudomonas putida
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/523 of 1pi3A

query
sites
1pi3A

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)
binding calcium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: G426 (= G92), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), N454 (= N121), T456 (≠ I123), Y457 (= Y124), G458 (= G125), A459 (≠ M126), L460 (≠ T127)

Sites not aligning to the query:

active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280, 375, 400, 402
binding magnesium ion: 116, 117, 119
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: 376, 377, 402

5deiA Benzoylformate decarboxylase from pseudomonas putida
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/524 of 5deiA

query
sites
5deiA

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)
binding calcium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)
binding thiamine diphosphate: G426 (= G92), D427 (= D93), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), T456 (≠ I123), Y457 (= Y124), G458 (= G125), A459 (≠ M126), L460 (≠ T127)

Sites not aligning to the query:

active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280, 375, 400, 402, 524
binding bicarbonate ion: 25, 69, 109
binding calcium ion: 185, 186
binding magnesium ion: 116, 117, 119
binding thiamine diphosphate: 22, 23, 46, 69, 376, 377, 402

3fsjX Crystal structure of benzoylformate decarboxylase in complex with the inhibitor mbp (see paper)
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/524 of 3fsjX

query
sites
3fsjX

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127)
binding calcium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G426 (= G92), D427 (= D93), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), T456 (≠ I123), Y457 (= Y124), G458 (= G125), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)

Sites not aligning to the query:

active site: 24, 25, 27, 46, 69, 108, 109, 110, 111, 280, 400, 524
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 280, 376, 377, 396, 400, 402, 523, 524

1mczA Benzoylformate decarboxylase from pseudomonas putida complexed with an inhibitor, r-mandelate (see paper)
31% identity, 48% coverage: 69:196/267 of query aligns to 403:520/524 of 1mczA

query
sites
1mczA

G

G

R

F

A

A

L

L

P

P

C

A

A

A

T

I

G

G

I

V

K

Q

M

L

T

A

K

E

P

P

E

E

L

R

N

Q

V

V

V

I

V

A

P

V

M

I

G
|
G

D
|
D

G
|
G

D
x
S

A

A

M

-

A

-

I

-

G

-

N

N

H
x
Y

F

S

I

I

H

S

-

A

-

L

-

W

-

T

A

A

C

A

R

Q

R

Y

N

N

I

I

D

P

M

T

T

I

A

F

I

V

V

I

L

M

N

N

N
|
N

R

G

I
x
T

Y
|
Y

G
|
G

M
x
A

T
x
L

G

R

G

W

Q
x
F

Y

A

S

G

P

V

L

L

S

E

G

A

R

E

G

N

V

V

L

P

A

G

T

L

T

D

A

V

P

P

Y

-

R

-

S

-

I

-

D

G

D

I

D

D

F

F

D

R

A

A

V

-

E

-

L

L

A

A

R

K

G

G

A

Y

G

G

A

-

T

-

F

-

V

V

A

Q

R

A

T

L

T

K

A

A

F

D

H

N

V

L

K

E

E

Q

L

L

S

K

K

G

I

S

L

L

T

Q

K

E

A

A

I

L

E

S

H

A

K

K

G

G

F

P

S

V

V

L

V

I

E

E

V

V

active site: D427 (= D93), N454 (= N121), T456 (≠ I123), Y457 (= Y124), A459 (≠ M126), L460 (≠ T127), F463 (≠ Q130)
binding magnesium ion: D427 (= D93), N454 (= N121), T456 (≠ I123)
binding (r)-mandelic acid: F463 (≠ Q130)
binding thiamine diphosphate: G426 (= G92), D427 (= D93), G428 (= G94), S429 (≠ D95), Y432 (≠ H103), Y457 (= Y124), G458 (= G125), A459 (≠ M126), L460 (≠ T127)

Sites not aligning to the query:

active site: 22, 24, 25, 26, 27, 46, 69, 108, 109, 110, 111, 159, 253, 280, 375, 400, 402, 524
binding magnesium ion: 116, 117, 119
binding (r)-mandelic acid: 25, 69, 109, 280, 376
binding thiamine diphosphate: 22, 23, 46, 69, 376, 377, 400, 402

Query Sequence

>WP_078717693.1 NCBI__GCF_900167125.1:WP_078717693.1
MAFRDLIRERFFPHIWCPGCGHGTVLNSLLHVVDKLGLDPTSMCMVSGIGCSARISGYVD
FHTMHTMHGRALPCATGIKMTKPELNVVVPMGDGDAMAIGGNHFIHACRRNIDMTAIVLN
NRIYGMTGGQYSPLSGRGVLATTAPYRSIDDDFDAVELARGAGATFVARTTAFHVKELSK
ILTKAIEHKGFSVVEVMVQCPTYFGRKNKMGGAVQLLEYYRDNTAPLGSKKLEENQNLIP
RGVFVERERPEYCEEYQNVIALAEGKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory