SitesBLAST
Comparing WP_078717838.1 NCBI__GCF_900167125.1:WP_078717838.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 13 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 82% coverage: 20:228/256 of query aligns to 21:218/291 of 6n2oD
- binding magnesium ion: D101 (= D99), N129 (= N127), I131 (≠ V129)
- binding succinyl-coenzyme a: I57 (= I55), R62 (≠ F60), L134 (≠ M132), K136 (≠ G134)
- binding iron/sulfur cluster: W24 (≠ Y23), C25 (= C24), C28 (= C27), C59 (= C57), C208 (= C218), T210 (= T220), F211 (≠ N221)
- binding thiamine diphosphate: I57 (= I55), G58 (= G56), C59 (= C57), S60 (= S58), H76 (= H74), G102 (= G100), D103 (= D101), N129 (= N127), I131 (≠ V129), G133 (= G131), L134 (≠ M132), T135 (= T133)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
33% identity, 82% coverage: 20:228/256 of query aligns to 21:218/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (≠ F60), L134 (≠ M132)
- binding coenzyme a: K136 (≠ G134), Y150 (≠ T148)
- binding magnesium ion: D101 (= D99), N129 (= N127), I131 (≠ V129)
- binding iron/sulfur cluster: W24 (≠ Y23), C25 (= C24), C28 (= C27), C59 (= C57), C208 (= C218), T210 (= T220), F211 (≠ N221)
- binding thiamine diphosphate: I57 (= I55), G58 (= G56), C59 (= C57), S60 (= S58), H76 (= H74), G102 (= G100), D103 (= D101), N129 (= N127), I131 (≠ V129), Y132 (= Y130), G133 (= G131), L134 (≠ M132), T135 (= T133)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
33% identity, 82% coverage: 20:228/256 of query aligns to 21:218/291 of 6n2nB
- binding magnesium ion: D101 (= D99), N129 (= N127), I131 (≠ V129)
- binding iron/sulfur cluster: W24 (≠ Y23), C25 (= C24), C28 (= C27), H30 (= H28), C59 (= C57), C208 (= C218), T210 (= T220), F211 (≠ N221)
- binding thiamine diphosphate: I57 (= I55), G58 (= G56), C59 (= C57), S60 (= S58), H76 (= H74), G100 (= G98), D101 (= D99), G102 (= G100), D103 (= D101), N129 (= N127), I131 (≠ V129), Y132 (= Y130), G133 (= G131), L134 (≠ M132), T135 (= T133)
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
31% identity, 77% coverage: 23:220/256 of query aligns to 8:196/301 of 5b46B
- binding magnesium ion: D87 (= D99), N115 (= N127), V117 (= V129)
- binding iron/sulfur cluster: W8 (≠ Y23), C9 (= C24), C12 (= C27), C43 (= C57), C194 (= C218), T196 (= T220)
- binding thiamine diphosphate: I41 (= I55), G42 (= G56), C43 (= C57), S44 (= S58), H62 (= H74), G86 (= G98), G88 (= G100), D89 (= D101), N115 (= N127), V117 (= V129), Y118 (= Y130), G119 (= G131), L120 (≠ M132), T121 (= T133)
Sites not aligning to the query:
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
31% identity, 77% coverage: 23:220/256 of query aligns to 11:199/304 of Q96XT4
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 55:58) binding thiamine diphosphate
- C46 (= C57) binding [4Fe-4S] cluster
- D90 (= D99) binding Mg(2+)
- GD 91:92 (= GD 100:101) binding thiamine diphosphate
- N118 (= N127) binding Mg(2+)
- V120 (= V129) binding Mg(2+)
- GL 122:123 (≠ GM 131:132) binding thiamine diphosphate
- C197 (= C218) binding [4Fe-4S] cluster
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
29% identity, 77% coverage: 23:220/256 of query aligns to 11:199/305 of Q96Y68
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- C46 (= C57) binding [4Fe-4S] cluster
- K49 (≠ F60) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D99) binding Mg(2+)
- GD 91:92 (= GD 100:101) binding thiamine diphosphate
- N118 (= N127) binding Mg(2+)
- V120 (= V129) binding Mg(2+)
- GL 122:123 (≠ GM 131:132) binding thiamine diphosphate
- K125 (≠ G134) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (= K194) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C218) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
29% identity, 77% coverage: 23:220/256 of query aligns to 11:199/305 of P72579
- K49 (≠ F60) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (≠ M132) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
29% identity, 77% coverage: 23:220/256 of query aligns to 4:181/275 of 5b47B
- binding magnesium ion: D83 (= D99), N111 (= N127)
- binding iron/sulfur cluster: C5 (= C24), C8 (= C27), C39 (= C57), C179 (= C218)
- binding thiamine diphosphate: I37 (= I55), G38 (= G56), C39 (= C57), S40 (= S58), H58 (= H74), G84 (= G100), D85 (= D101), N111 (= N127), V113 (= V129), Y114 (= Y130), L116 (≠ M132)
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
29% identity, 77% coverage: 23:220/256 of query aligns to 7:185/286 of 5b48B
- binding magnesium ion: D84 (= D99), N112 (= N127), V114 (= V129)
- binding iron/sulfur cluster: C8 (= C24), C11 (= C27), C42 (= C57), C183 (= C218), T185 (= T220)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I55), G41 (= G56), C42 (= C57), S43 (= S58), H59 (= H74), G85 (= G100), D86 (= D101), N112 (= N127), V114 (= V129), Y115 (= Y130), G116 (= G131), L117 (≠ M132)
9bt4C Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
24% identity, 78% coverage: 24:222/256 of query aligns to 13:210/291 of 9bt4C
- binding magnesium ion: D94 (= D99), N122 (= N127), A124 (≠ V129)
- binding iron/sulfur cluster: C13 (= C24), C16 (= C27), C17 (≠ H28), D18 (≠ H29), C41 (= C57), M126 (≠ G131), C206 (= C218), T208 (= T220)
- binding thiamine diphosphate: D18 (≠ H29), T39 (≠ I55), G40 (= G56), C41 (= C57), F63 (≠ H74), E64 (≠ G75), G93 (= G98), D94 (= D99), G95 (= G100), S96 (≠ D101), N122 (= N127), A124 (≠ V129), Y125 (= Y130), M126 (≠ G131), N127 (≠ M132), T128 (= T133)
5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
23% identity, 79% coverage: 24:225/256 of query aligns to 24:232/310 of 5exdF
- active site: N143 (≠ M132)
- binding magnesium ion: D110 (= D99), N138 (= N127), S140 (≠ V129)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I55), G51 (= G56), C52 (= C57), I74 (≠ A79), D110 (= D99), G111 (= G100), Y136 (≠ V125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ H29), C52 (= C57), A142 (≠ G131), C225 (= C218), K227 (≠ T220)
5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
23% identity, 79% coverage: 24:225/256 of query aligns to 24:232/314 of 5exeC
- active site: N143 (≠ M132)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I55), G51 (= G56), C52 (= C57), I74 (≠ A79), G109 (= G98), D110 (= D99), G111 (= G100), Y136 (≠ V125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)
- binding magnesium ion: D110 (= D99), D130 (≠ R119), N138 (= N127), S140 (≠ V129), L211 (≠ Q203), Q213 (= Q205)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ H29), C52 (= C57), C225 (= C218), P226 (= P219), K227 (≠ T220)
5c4iC Structure of an oxalate oxidoreductase (see paper)
23% identity, 79% coverage: 24:225/256 of query aligns to 24:232/312 of 5c4iC
- active site: N143 (≠ M132)
- binding magnesium ion: D110 (= D99), N138 (= N127), S140 (≠ V129)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ H29), C52 (= C57), A142 (≠ G131), C225 (= C218), P226 (= P219), K227 (≠ T220)
- binding thiamine diphosphate: T50 (≠ I55), G51 (= G56), C52 (= C57), I74 (≠ A79), G109 (= G98), D110 (= D99), G111 (= G100), Y136 (≠ V125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)
Query Sequence
>WP_078717838.1 NCBI__GCF_900167125.1:WP_078717838.1
MSEMQEKLVFDRPESVIDRPTHYCPGCHHGVAHRLVGELLDEMKLREESILIGSIGCSVF
LYNYLDIDAVEAPHGRAPAVGTGAKRARPDKFVLTYQGDGDLASIGMAEIMHAANRGERI
CVVFVNNTVYGMTGGQMAPTTLVGQRTTTCPGGRCRDKEGLPIKMTEIISQLGGVAFAGR
ASLDSVKNIRTAKKYLRKAFECQTQDRGFGFVELLSACPTNWKMPPVQANERIREEMIPY
FPLGVYKDVTEEGGAC
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory