SitesBLAST
Comparing WP_078718056.1 NCBI__GCF_900167125.1:WP_078718056.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
A0A0H3JRU9 Pyruvate carboxylase; EC 6.4.1.1 from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see 2 papers)
30% identity, 96% coverage: 16:1204/1233 of query aligns to 5:1145/1150 of A0A0H3JRU9
- R21 (= R32) mutation to A: Complete loss of catalytic activity.
- K119 (= K128) binding ATP
- K161 (= K169) binding ATP
- H211 (= H219) binding ATP
- E278 (= E288) binding ATP
- K411 (≠ L415) mutation to A: Complete loss of catalytic activity.
- RDAHQ 541:545 (≠ RDITQ 552:556) binding substrate
- D542 (= D553) binding Mn(2+)
- A580 (= A589) mutation to T: Complete loss of catalytic activity.
- R614 (= R623) mutation to A: Complete loss of catalytic activity.
- Y621 (= Y630) mutation to A: Complete loss of catalytic activity.
- K712 (= K727) binding Mn(2+)
- H741 (= H757) binding Mn(2+)
- H743 (= H759) binding Mn(2+)
- Q838 (≠ A856) mutation to A: About 2.5-fold loss of catalytic activity.
- T876 (= T908) mutation to A: Complete loss of catalytic activity.
- S879 (≠ H911) mutation to A: About 2-fold loss of catalytic activity.
- K880 (= K912) mutation to T: Complete loss of catalytic activity.
7wtdC Cryo-em structure of human pyruvate carboxylase with acetyl-coa in the intermediate state 1 (see paper)
30% identity, 96% coverage: 16:1204/1233 of query aligns to 6:1145/1146 of 7wtdC
- binding adenosine-5'-triphosphate: K162 (= K169), G167 (= G174), G168 (= G175), F206 (≠ L213), Q236 (= Q243), H239 (= H246), E292 (= E301)
- binding coenzyme a: F21 (≠ C31), R22 (= R32), T25 (≠ S35), R45 (≠ P54), Q46 (≠ A55), K47 (≠ T56), A48 (≠ S57), D49 (≠ G58), E50 (≠ A59), R366 (≠ F372), R413 (= R418), A416 (≠ R421), R419 (≠ V424), Q462 (≠ A468), R464 (≠ D470), A465 (≠ S471), Q466 (≠ F472), K1024 (= K1075), R1053 (≠ L1104)
7wtbB Cryo-em structure of human pyruvate carboxylase with acetyl-coa (see paper)
30% identity, 96% coverage: 16:1204/1233 of query aligns to 7:1146/1147 of 7wtbB
- binding acetyl coenzyme *a: F22 (≠ C31), T26 (≠ S35), R46 (≠ P54), Q47 (≠ A55), K48 (≠ T56), A49 (≠ S57), D50 (≠ G58), R367 (≠ F372), R414 (= R418), E418 (= E422), R420 (≠ V424), R422 (≠ G426), A462 (≠ E467), Q463 (≠ A468), R465 (≠ D470), K1025 (= K1075)
- binding phosphoaminophosphonic acid-adenylate ester: K163 (= K169), G168 (= G174), G169 (= G175), M173 (≠ I179), F207 (≠ L213), I208 (≠ L214), P211 (≠ V217), H240 (= H246)
- binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: D582 (≠ H592), Q839 (≠ A856), T877 (= T893), S880 (= S896), K881 (≠ Q897)
P11498 Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; EC 6.4.1.1 from Homo sapiens (Human) (see 6 papers)
30% identity, 96% coverage: 16:1204/1233 of query aligns to 38:1177/1178 of P11498