SitesBLAST
Comparing WP_079557079.1 NCBI__GCF_002201795.1:WP_079557079.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3rc1A Crystal structure of kijd10, a 3-ketoreductase from actinomadura kijaniata incomplex with NADP and tdp-benzene (see paper)
28% identity, 97% coverage: 4:321/328 of query aligns to 3:320/325 of 3rc1A
- active site: K95 (= K97), Y179 (= Y181)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C10 (≠ V11), A11 (≠ S12), D12 (≠ N13), I13 (≠ H14), S35 (= S36), R36 (= R37), Y56 (= Y58), P71 (= P73), L72 (= L74), P73 (= P75), H77 (= H79), E94 (= E96), K95 (= K97), I162 (= I164), R163 (= R165), V168 (≠ Y170), D175 (= D177), Y179 (= Y181)
- binding phosphate ion: H278 (≠ S279), K283 (≠ L284)
- binding 5'-O-[(S)-hydroxy{[(S)-hydroxy(phenoxy)phosphoryl]oxy}phosphoryl]thymidine: R16 (≠ K17), R17 (= R18), F152 (= F154), I154 (≠ Y156), P155 (≠ N157), Y233 (≠ F235), T253 (≠ N254)
B3TMR8 dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase; 3-ketoreductase; NADPH-dependent C3-ketoreductase; EC 1.1.1.384 from Actinomadura kijaniata (see paper)
28% identity, 97% coverage: 4:321/328 of query aligns to 10:327/332 of B3TMR8
- 17:23 (vs. 11:17, 0% identical) binding NADP(+)
- SR 42:43 (= SR 36:37) binding NADP(+)
- Y63 (= Y58) binding NADP(+)
- L79 (= L74) binding NADP(+)
- H84 (= H79) binding NADP(+)
- K102 (= K97) active site, Proton donor; mutation K->A,M,Q: Loss of reductase activity.; mutation to E: Retains some activity, but the catalytic efficiency is strongly reduced.
- R170 (= R165) binding NADP(+)
- D182 (= D177) binding NADP(+)
- Y186 (= Y181) mutation to F: Same affinity for dTDP-glucose and NADPH compared to the wild-type. Small reduction of the catalytic efficiency resulting from the conformational flexibility of the nicotinamide ring.
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
25% identity, 98% coverage: 3:323/328 of query aligns to 33:368/383 of 1h6dA
- active site: K131 (= K97), Y219 (= Y181)
- binding glycerol: K131 (= K97), R202 (≠ I164), D215 (= D177), Y219 (= Y181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G10), L41 (≠ V11), G42 (≠ S12), K43 (≠ N13), Y44 (≠ H14), S66 (= S36), G67 (≠ R37), K71 (≠ Q41), Y89 (≠ H55), I107 (≠ P73), L108 (= L74), P109 (= P75), N110 (= N76), H113 (= H79), E130 (= E96), K131 (= K97), R159 (≠ M125), A198 (≠ S160), W201 (≠ N163), R202 (≠ I164), Y219 (= Y181), Y298 (= Y256)
Sites not aligning to the query:
1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
25% identity, 98% coverage: 3:323/328 of query aligns to 31:366/381 of 1rydA
- active site: K129 (= K97), Y217 (= Y181)
- binding alpha-D-glucopyranose: Y236 (≠ M200), I254 (= I216), Q256 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ V11), G40 (≠ S12), K41 (≠ N13), Y42 (≠ H14), S64 (= S36), G65 (≠ R37), K69 (≠ Q41), Y87 (≠ H55), L106 (= L74), P107 (= P75), N108 (= N76), L110 (= L78), H111 (= H79), E128 (= E96), K129 (= K97), R157 (≠ M125), A196 (≠ S160), W199 (≠ N163), R200 (≠ I164), Y217 (= Y181), Y296 (= Y256)
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
24% identity, 98% coverage: 3:323/328 of query aligns to 2:325/340 of 1evjA
- active site: K100 (= K97), Y188 (= Y181)
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), L10 (≠ V11), G11 (≠ S12), K12 (≠ N13), Y13 (≠ H14), D35 (≠ S36), L77 (= L74), P78 (= P75), N79 (= N76), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M125), W170 (≠ N163), R171 (≠ I164), Y188 (= Y181), Y267 (≠ T255)
5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 2:335/335 of 5a05A
- active site: K100 (= K97), Y185 (= Y181)
- binding beta-D-glucopyranose: K100 (= K97), F159 (≠ Y156), D181 (= D177), Y185 (= Y181)
- binding alpha-D-glucopyranose: P259 (= P252), S262 (≠ T255)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), L10 (≠ V11), G11 (≠ N13), Y12 (≠ H14), Y13 (≠ L15), S35 (= S36), G36 (≠ R37), T37 (≠ K38), K40 (≠ Q41), Y58 (vs. gap), I76 (≠ P73), T77 (≠ L74), P78 (= P75), N79 (= N76), L81 (= L78), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M125), W167 (≠ I164), R168 (= R165), Y185 (= Y181), Y263 (= Y256)
5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 2:335/335 of 5a04A
- active site: K100 (= K97), Y185 (= Y181)
- binding beta-D-glucopyranose: K100 (= K97), F159 (≠ Y156), R168 (= R165), D181 (= D177), Y185 (= Y181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L10 (≠ V11), G11 (≠ N13), Y12 (≠ H14), Y13 (≠ L15), S35 (= S36), G36 (≠ R37), T37 (≠ K38), K40 (≠ Q41), Y58 (vs. gap), I76 (≠ P73), T77 (≠ L74), P78 (= P75), N79 (= N76), L81 (= L78), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M125), W167 (≠ I164), R168 (= R165), Y185 (= Y181), Y263 (= Y256)
5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 2:335/335 of 5a03E
- active site: K100 (= K97), Y185 (= Y181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), L10 (≠ V11), G11 (≠ N13), Y12 (≠ H14), Y13 (≠ L15), S35 (= S36), G36 (≠ R37), T37 (≠ K38), K40 (≠ Q41), Y58 (vs. gap), I76 (≠ P73), T77 (≠ L74), P78 (= P75), N79 (= N76), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M125), W167 (≠ I164), R168 (= R165), Y185 (= Y181), Y263 (= Y256)
- binding beta-D-xylopyranose: K100 (= K97), F159 (≠ Y156), R168 (= R165), D181 (= D177), Y185 (= Y181), E205 (≠ L198), T207 (≠ M200), R209 (≠ K202)
- binding alpha-D-xylopyranose: H134 (≠ M131), M268 (≠ A261), R279 (= R272), E280 (≠ V273)
5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 2:335/335 of 5a02A
- active site: K100 (= K97), Y185 (= Y181)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L10 (≠ V11), G11 (≠ N13), Y12 (≠ H14), Y13 (≠ L15), S35 (= S36), G36 (≠ R37), T37 (≠ K38), K40 (≠ Q41), Y58 (vs. gap), I76 (≠ P73), T77 (≠ L74), P78 (= P75), L81 (= L78), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M125), W167 (≠ I164), R168 (= R165), Y185 (= Y181), Y263 (= Y256)
5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 3:336/336 of 5a06A
- active site: K101 (= K97), Y186 (= Y181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L11 (≠ V11), G12 (≠ N13), Y13 (≠ H14), Y14 (≠ L15), S36 (= S36), G37 (≠ R37), T38 (≠ K38), K41 (≠ Q41), Y59 (vs. gap), I77 (≠ P73), T78 (≠ L74), P79 (= P75), N80 (= N76), L82 (= L78), H83 (= H79), E100 (= E96), K101 (= K97), R129 (≠ M125), W168 (≠ I164), R169 (= R165), Y186 (= Y181), Y264 (= Y256)
- binding sorbitol: D72 (= D68), H96 (≠ P92), K101 (= K97), R122 (≠ I118), R122 (≠ I118), L124 (≠ F120), F160 (≠ Y156), R169 (= R165), D182 (= D177), Y186 (= Y181), K287 (≠ S279), H296 (≠ A288), E299 (= E291), E306 (≠ P298), G310 (≠ E302), G311 (≠ T303)
Sites not aligning to the query:
5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
24% identity, 99% coverage: 3:328/328 of query aligns to 3:336/336 of 5a03C
- active site: K101 (= K97), Y186 (= Y181)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), L11 (≠ V11), G12 (≠ N13), Y13 (≠ H14), Y14 (≠ L15), S36 (= S36), G37 (≠ R37), T38 (≠ K38), K41 (≠ Q41), Y59 (vs. gap), I77 (≠ P73), T78 (≠ L74), P79 (= P75), N80 (= N76), L82 (= L78), H83 (= H79), E100 (= E96), K101 (= K97), R129 (≠ M125), W168 (≠ I164), R169 (= R165), Y186 (= Y181), Y264 (= Y256)
- binding beta-D-xylopyranose: K101 (= K97), F160 (≠ Y156), R169 (= R165), D182 (= D177), Y186 (= Y181)
2poqX Dimeric dihydrodiol dehydrogenase complexed with inhibitor, isoascorbic acid (see paper)
26% identity, 66% coverage: 31:245/328 of query aligns to 30:244/331 of 2poqX
Sites not aligning to the query:
2o4uX Crystal structure of mammalian dimeric dihydrodiol dehydrogenase (see paper)
26% identity, 66% coverage: 31:245/328 of query aligns to 30:244/331 of 2o4uX