SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_079558836.1 NCBI__GCF_002201795.1:WP_079558836.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 98% coverage: 3:605/613 of query aligns to 17:573/575 of P9WKJ5

query
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P9WKJ5

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C64 (≠ G50) binding [2Fe-2S] cluster
D96 (= D82) binding Mg(2+)
C137 (= C123) binding [2Fe-2S] cluster
D138 (= D124) binding Mg(2+)
K139 (= K125) binding via carbamate group; modified: N6-carboxylysine
C214 (= C192) binding [2Fe-2S] cluster
E465 (= E488) binding Mg(2+)
S491 (= S514) mutation to A: Loss of catalytic activity.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
36% identity, 98% coverage: 3:605/613 of query aligns to 4:560/562 of 6ovtA

query
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6ovtA

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binding fe2/s2 (inorganic) cluster: C51 (≠ G50), G84 (= G83), C124 (= C123), D125 (= D124), A200 (≠ S191), C201 (= C192)
binding magnesium ion: D83 (= D82), D125 (= D124), K126 (= K125), E452 (= E488)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 91% coverage: 7:566/613 of query aligns to 57:575/608 of Q9LIR4

query
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Q9LIR4

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E

F

G

V

N

P

T

G
x
C

H

N

A

M

H

H

L

L

H

L

Q

K

I

L

G

S

Q

E

D

A

V

V

K

K

R

E

M

G

I

V

E

E

K

N

Q

A

G

G

C

M

F

V

A

G

A

F

E

R

F

F

N

N

T

T

I

I

A

G

I

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

Q

W

A

Y

D

D

A

G

M

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

V

F

G

Q

D

D

K

K

A

T

L

Y

D

D

L

I

V

V

D

S

S

A

M

F

V

Q

M

S

A

Y

A

G

D

E

-

F

-

V

-

S

D

G

S

S

V

I

S

S

D

D

E

E

E

Q

L

R

L

K

A

T

V

V

E

L

Q

H

N

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

N

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

V

P

A

A

T

E

H

-

V

-

N

D

R

P

K

L

K

K

L

L

F

D

E

E

K

-

A

-

A

-

N

-

Q

-

I

-

V

C

R

R

L

L

A

A

Y

G

K

K

Y

Y

Y

L

Q

L

D

E

G

L

D

L

E

K

S

M

V

D

L

L

-

K

P

P

R

R

N

D

I

I

A

I

T

T

K

P

A

K

A

S

F

L

N

R

N

N

S

A

M

M

T

V

L

S

D

V

V

M

A

A

M

L

G

G

G

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

L

L

L

I

A

A

I

I

A

A

H

R

E

S

A

V

G

G

V

L

D

E

F

L

T

T

M

L

Q

D

D

D

I

F

D

Q

R

K

I

V

S

S

R

D

K

A

T

V

P

P

V

F

L

L

C

A

K

D

V

L

A

K

P

P

N

S

S

G

D

K

Y

Y

H

V

I

M

E

E

D

D

V

I

N

H

R

K

A

I

G

G

G

G

I

T

M

P

G

A

I

V

L

L

A

R

E

Y

L

L

N

L

R

E

G

L

G

G

L

L

L

M

D

D

A

G

T

D

V

C

G

M

R

T

V

V

D

T

Y

G

S

Q

S

T

L

L

S

A

E

Q

A

N

L

L

D

E

A

-

Y

-

D

-

M

-

A

-

S

N

K

V

S

P

A

S

I

L

A

T

E

E

A

G

K

Q

E

E

I

I

Y

-

Q

-

S

-

A

-

P

-

G

-

G

-

F

-

V

-

N

-

L

-

V

-

M

-

G

-

S

-

Q

-

K

-

A

-

I

-

Y

-

G

-

E

-

L

-

D

-

L

-

D

-

R

-

E

-

K

-

G

-

C

-

I

I

R

R

N

P

I

L

S

S

H

N

A

P

Y

I

S

K

Q

E

D

T

G

G

G

H

L

I

A

Q

V

I

L

L

F

R

G

G

N

D

I

L

A

A

E

P

N

D

G

G

C

S

I

V

V

A

K

K

T

I

A

T

G

G

V

K

D

E

E

G

S

-

I

-

F

L

H

Y

F

F

E

S

G

G

T

P

A

A

R

L

V

V

Y

F

E

E

S

G

Q

E

D

E

D

S

A

M

C

L

D

A

G

A

I

I

L

S

S

A

G

D

E

P

V

M

K

S

-

F

A

K

G

G

D

T

V

V

V

V

I

V

I

I

K

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E
|
E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

L

I

K

M

S

G

R

A

N

G

L

L

G

G

A

K

K

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

A

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

A

G

L

L

I

I

K

K

N

N

G

G

D

D

K

I

V

I

I

T

I

I

D

D

I

I

P

G

Q

K

R

K

S

R

I

I

N

D

I

T

D

Q

V

V

T

S

E

P

D

E

E

E

L

M

N

N

Q

D

R

R

R

R

C100 (≠ G50) binding [2Fe-2S] cluster
C173 (= C123) binding [2Fe-2S] cluster
D174 (= D124) binding Mg(2+)
C245 (= C192) binding [2Fe-2S] cluster
E497 (= E488) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
36% identity, 91% coverage: 7:566/613 of query aligns to 19:537/570 of 8hs0A

query
sites
8hs0A

S

S

N

S

T

R

T

I

T

T

Q

E

G

P

R

K

R

S

M

Q

A

G

G

G

A

S

R

Q

S

A

L

I

W

L

R

H

A

G

N

V

G

G

M

L

K

S

D

D

E

D

Q

D

M

L

G

L

R

K

P

P

L

Q

I

I

A

G

V

I

V

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V

N

P

T

G
x
C

H

N

A

M

H

H

L

L

H

L

Q

K

I

L

G

S

Q

E

D

A

V

V

K

K

R

E

M

G

I

V

E

E

K

N

Q

A

G

G

C

M

F

V

A

G

A

F

E

R

F

F

N

N

T

T

I

I

A

G

I

V

D

S

D
|
D

G
x
A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

Q

W

A

Y

D

D

A

G

M

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K
|
K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

V

F

G

Q

D

D

K

K

A

T

L

Y

D

D

L

I

V

W

D

S

S

A

M

F

V

Q

M

S

A

Y

A

G

D

E

-

F

-

V

-

S

D

G

S

S

V

I

S

S

D

D

E

E

E

Q

L

R

L

K

A

T

V

V

E

L

Q

H

N

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

N

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

V

P

A

A

T

E

H

-

V

-

N

D

R

P

K

L

K

K

L

L

F

D

E

E

K

-

A

-

A

-

N

-

Q

-

I

-

V

C

R

R

L

L

A

A

Y

G

K

K

Y

Y

Y

L

Q

L

D

E

G

L

D

L

E

K

S

M

V

D

L

L

-

K

P

P

R

R

N

D

I

I

A

I

T

T

K

P

A

K

A

S

F

L

N

R

N

N

S

A

M

M

T

V

L

S

D

V

V

M

A

A

M

L

G

G

G

G

S

S

T

T

N
|
N

T

A

V

V

L

L

H
|
H

L

L

L

I

A

A

I

I

A

A

H

R

E

S

A

V

G

G

V

L

D

E

F

L

T

T

M

L

Q

D

D

D

I

F

D

Q

R

K

I

V

S

S

R

D

K

A

T

V

P

P

V

F

L

L

C

A

K

D

V

L

A

K

P

P

N

S

S

G

D

K

Y

Y

H

V

I

M

E

E

D

D

V

I

N

H

R

K

A

I

G

G

G

G

I

T

M

P

G

A

I

V

L

L

A

R

E

Y

L

L

N

L

R

E

G

L

G

G

L

L

L

M

D

D

A

G

T

D

V

C

G

M

R

T

V

V

D

T

Y

G

S

Q

S

T

L

L

S

A

E

Q

A

N

L

L

D

E

A

-

Y

-

D

-

M

-

A

-

S

N

K

V

S

P

A

S

I

L

A

T

E

E

A

G

K

Q

E

E

I

I

Y

-

Q

-

S

-

A

-

P

-

G

-

G

-

F

-

V

-

N

-

L

-

V

-

M

-

G

-

S

-

Q

-

K

-

A

-

I

-

Y

-

G

-

E

-

L

-

D

-

L

-

D

-

R

-

E

-

K

-

G

-

C

-

I

I

R

R

N

P

I

L

S

S

H

N

A

P

Y

I

S

K

Q

E

D

T

G

G

G

H

L

I

A

Q

V

I

L

L

F

R

G

G

N

D

I

L

A

A

E

P

N

D

G

G

C

S

I

V

V

A

K

K

T

I

A

T

G

G

V

K

D

E

E

G

S

-

I

-

F

L

H

Y

F

F

E

S

G

G

T

P

A

A

R

L

V

V

Y

F

E

E

S

G

Q

E

D

E

D

S

A

M

C

L

D

A

G

A

I

I

L

S

S

A

G

D

E

P

V

M

K

S

-

F

A

K

G

G

D

T

V

V

V

V

I

V

I

I

K

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

L

I

K

M

S

G

R

A

N

G

L

L

G

G

A

K

K

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

A

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

A

G

L

L

I

I

K

K

N

N

G

G

D

D

K

I

V

I

I

T

I

I

D

D

I

I

P

G

Q

A

R

A

S

R

I

I

N

D

I

T

D

Q

V

V

T

S

E

P

D

E

E

E

L

M

N

N

Q

D

R

R

R

R

binding fe2/s2 (inorganic) cluster: C62 (≠ G50), D94 (= D82), A95 (≠ G83), C135 (= C123), D136 (= D124), C207 (= C192)
binding magnesium ion: D136 (= D124), K137 (= K125), N285 (= N277), H289 (= H281)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
34% identity, 91% coverage: 7:566/613 of query aligns to 16:490/523 of 8imuA

query
sites
8imuA

S

S

N

S

T

R

T

I

T

T

Q

E

G

P

R

K

R

S

M

Q

A

G

G

G

A

S

R

Q

S

A

L

I

W

L

R

H

A

G

N

V

G

G

M

L

K

S

D

D

E

D

Q

D

M

L

G

L

R

K

P

P

L

Q

I

I

A

G

V

I

V

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V
x
N

P

T

G
x
C

H

N

A

M

H

H

L

L

H

L

Q

K

I

L

G

S

Q

E

D

A

V

V

K

K

R

E

M

G

I

V

E

E

K

N

Q

A

G

G

C

M

F

V

A

G

A

F

E

R

F

F

N

N

T

T

I

I

A

G

I

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

D

D

L

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

Q

W

A

Y

D

D

A

G

M

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

M

G

R

R

I

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

-

V

-

G

-

D

-

K

-

A

-

L

-

D

-

L

-

V

-

D

-

S

-

M

-

V

-

M

-

A

-

A

-

D

-

D

-

S

-

V

-

S

-

D

-

E

-

E

-

L

-

L

-

A

-

V

-

E

-

Q

-

N

-

A

-

C

-

P

-

T

-

C

-

G

-

S

-

C

-

S

-

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

N

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

T

S

I

I

V

P

A

A

T

E

H

-

V

-

N

D

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A

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C

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Y

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binding fe2/s2 (inorganic) cluster: N57 (≠ V48), C59 (≠ G50), C132 (= C123), D133 (= D124)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
32% identity, 89% coverage: 20:565/613 of query aligns to 25:529/583 of Q1JUQ1

query
sites
Q1JUQ1

R

R

S

S

L

W

W

M

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A

N

N

Q

G

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C47 (≠ V41) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (≠ G50) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C123) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C192) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ E478) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

9evvD His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
30% identity, 93% coverage: 34:604/613 of query aligns to 39:557/575 of 9evvD

query
sites
9evvD

G

G

R

R

P

P

L

V

I

I

A

G

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|
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I

A

V

V

V

L

L

F

R

G

G

N

N

I

L

A

A

E

P

N

K

G

G

C

A

I

V

V

L

K

K

T

P

A

S

G

A

V

A

D

S

E

P

S

H

I

L

F

L

H

V

F

H

E

K

G

G

T

R

A

A

R

V

V

V

Y

F

E

E

S

D

Q

I

D

D

D

D

A

Y

C

K

D

A

G

K

I

I

L

N

S

D

G

D

E

N

V

L

K

D

A

I

G

D

D

E

-

N

-

C

V

I

V

M

I

V

I

M

K

K

Y

N

E

C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

-

V

-

G

-

N

M

M

L

G

Y

L

P

P

T

P

S

K

Y

V

L

L

K

K

S

K

R

G

N

I

L

L

G

-

A

-

K

D

C

M

A

V

L

R

L

I

T

S

D

D

G

A

R

R

F

M

S
|
S

G
|
G

G

T

T

A

S

Y

G

G

L

T

S

V

I

V

G

L

H

H

A

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

A

P

I

L

A

A

L

V

I

V

K

K

N

N

G

G

D

D

K

M

V

I

I

E

I

L

D

D

I

V

P

P

Q

N

R

R

S

R

I

L

N

H

I

L

D

D

V

I

T

S

E

D

D

E

E

E

L

L

N

-

Q

A

R

R

R

R

A

L

A

A

E

E

D

-

A

-

K

-

G

-

D

-

N

-

A

-

W

W

K

Q

P

P

S

N

A

-

R

H

E

D

R

L

F

P

V

T

S

S

K

G

A

Y

L

A

R

F

A

L

Y

H

A

Q

G

Q

M

H

V

V

T

E

S

G

A

A

D

D

T

T

G

G

A

A

binding 2-ketobutyric acid: E87 (≠ D82), D124 (= D124), T201 (= T197), E449 (= E488), S476 (= S514), G477 (= G515)
binding fe2/s2 (inorganic) cluster: C55 (≠ G50), C123 (= C123), D124 (= D124), T195 (≠ S191), C196 (= C192)
binding magnesium ion: E87 (≠ D82), D124 (= D124), K125 (= K125), E449 (= E488)

9evvA His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
30% identity, 93% coverage: 34:604/613 of query aligns to 39:557/575 of 9evvA

query
sites
9evvA

G

G

R

R

P

P

L

V

I

I

A

G

V

I

V

L

N

N

S

T

F

W

T

S

Q

D

F

M

V

T

P

P

G
x
C

H

N

A

G

H

H

L

L

H

R

Q

E

I

L

G

A

Q

E

D

K

V

V

K

K

R

A

M

G

I

V

E

W

K

E

Q

A

G

G

C

G

F

F

A

P

A

L

E

E

F

V

N

P

T

V

I

F

A

S

I

A

D

S

D
x
E

G
x
N

I

-

A

T

M

F

G

R

H

P

D

T

G

A

M

M

L

M

Y

Y

S

-

L

-

P

-

S

-

R

R

D

N

L

L

I

A

A

A

D

L

S

A

V

V

E

E

Y

E

M

A

V

I

N

R

A

G

H

Q

Q

P

A

M

D

D

A

G

M

C

V

V

C

L

I

L

S

V

N

G

C
|
C

D
|
D

K
|
K

I

T

T

T

P

P

G

S

M

L

L

L

M

M

A

G

A

A

M

A

R

S

I

C

N

D

I

L

P

P

V

S

I

I

F

V

V

V

S

T

G

G

G

G

P

P

M

M

E

L

A

N

G

G

-

Y

-

F

-

R

-

G

-

E

K

R

V

V

G

G

D

S

K

G

A

T

-

H

L

L

D

W

L

K

V

F

D

S

S

E

M

M

V

V

M

K

A

A

A

G

D

E

D

-

S

-

V

M

S

T

D

Q

E

A

E

E

L

F

L

L

A

E

V

A

E

E

Q

A

N

S

A

M

C

S

P

R

T

S

C

S

G

G

S
x
T

C
|
C

S

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

N

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

G

G

N

N

G

A

T

A

I

I

V

P

A

G

T

V

H

D

V

S

N

R

R

R

K

K

K

V

L

M

F

A

E

Q

K

L

A

T

A

G

N

R

Q

R

I

I

V

V

R

Q

L

M

A

V

Y

-

K

-

Y

-

Y

-

Q

-

D

-

G

-

D

K

E

D

S

D

V

L

L

K

P

P

R

S

N

E

I

I

A

M

T

T

K

K

A

Q

A

A

F

F

N

E

N

N

S

A

M

I

T

R

L

T

D

N

V

A

A

A

M

I

G

G

G

G

S

S

T

T

N

N

T

A

V

V

L

I

H

H

L

L

L

L

A

A

I

I

A

A

H

G

E

R

A

V

G

G

V

I

D

D

F

L

T

S

M

L

Q

D

D

D

I

W

D

D

R

R

I

C

S

G

R

R

K

D

T

V

P

P

V

T

L

I

C

V

K

N

V

L

A

M

P

P

N

S

S

G

D

K

Y

Y

H

L

I

M

E

E

D

E

V

F

N

F

R

Y

A

A

G

G

G

G

I

L

M

P

G

V

I

V

L

L

A

K

E

R

L

L

N

G

R

E

G

A

G

G

L

L

L

L

D

H

A

K

T

-

V

-

G

-

R

-

V

-

D

-

Y

-

S

-

S

-

L

-

S

-

E

-

A

-

L

-

D

D

A

A

Y

L

D

T

M

V

A

S

S

G

K

E

S

T

A

V

I

W

A

D

E

E

A

V

K

K

E

D

I

V

Y

V

Q

N

S

W

A

N

P

E

G

-

G

-

F

-

V

-

N

D

L

V

V

I

M

L

G

P

S

A

Q

E

K

K

A

A

I

L

Y

-

G

-

E

-

L

-

D

-

L

-

D

-

R

-

E

-

K

-

G

-

C

-

I

-

R

-

N

-

I

-

S

-

H

-

A

-

Y

-

S

T

Q

S

D

S

G

G

G

G

L

I

A

V

V

V

L

L

F

R

G

G

N

N

I

L

A

A

E

P

N

K

G

G

C

A

I

V

V

L

K

K

T

P

A

S

G

A

V

A

D

S

E

P

S

H

I

L

F

L

H

V

F

H

E

K

G

G

T

R

A

A

R

V

V

V

Y

F

E

E

S

D

Q

I

D

D

D

D

A

Y

C

K

D

A

G

K

I

I

L

N

S

D

G

D

E

N

V

L

K

D

A

I

G

D

D

E

-

N

-

C

V

I

V

M

I

V

I

M

K

K

Y

N

E

C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

Q

A

E
|
E

-

V

-

G

-

N

M

M

L

G

Y

L

P

P

T

P

S

K

Y

V

L

L

K

K

S

K

R

G

N

I

L

L

G

-

A

-

K

D

C

M

A

V

L

R

L

I

T

S

D

D

G

A

R

R

F

M

S

S

G

G

G

T

T

A

S

Y

G

G

L

T

S

V

I

V

G

L

H

H

A

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

A

P

I

L

A

A

L

V

I

V

K

K

N

N

G

G

D

D

K

M

V

I

I

E

I

L

D

D

I

V

P

P

Q

N

R

R

S

R

I

L

N

H

I

L

D

D

V

I

T

S

E

D

D

E

E

E

L

L

N

-

Q

A

R

R

R

R

A

L

A

A

E

E

D

-

A

-

K

-

G

-

D

-

N

-

A

-

W

W

K

Q

P

P

S

N

A

-

R

H

E

D

R

L

F

P

V

T

S

S

K

G

A

Y

L

A

R

F

A

L

Y

H

A

Q

G

Q

M

H

V

V

T

E

S

G

A

A

D

D

T

T

G

G

A

A

binding fe2/s2 (inorganic) cluster: C55 (≠ G50), N88 (≠ G83), C123 (= C123), D124 (= D124), T195 (≠ S191), C196 (= C192)
binding magnesium ion: E87 (≠ D82), D124 (= D124), K125 (= K125), E449 (= E488)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
30% identity, 93% coverage: 34:604/613 of query aligns to 43:561/579 of B5ZZ34

query
sites
B5ZZ34

G

G

R

R

P

P

L

V

I

I

A

G

V

I

V

L

N

N

S

T

F

W

T

S

Q

D

F

M

V

T

P

P

G
x
C

H

N

A

G

H

H

L

L

H

R

Q

E

I

L

G

A

Q

E

D

K

V

V

K

K

R

A

M

G

I

V

E

W

K

E

Q

A

G

G

C

G

F

F

A

P

A

L

E

E

F

V

N

P

T

V

I

F

A

S

I

A

D

S

D
x
E

G

N

I

-

A

T

M

F

G

R

H

P

D

T

G

A

M

M

L

M

Y

Y

S

-

L

-

P

-

S

-

R

R

D

N

L

L

I

A

A

A

D

L

S

A

V

V

E

E

Y

E

M

A

V

I

N

R

A

G

H

Q

Q

P

A

M

D

D

A

G

M

C

V

V

C

L

I

L

S

V

N

G

C
|
C

D
|
D

K

K

I

T

T

T

P

P

G

S

M

L

L

L

M

M

A

G

A

A

M

A

R

S

I

C

N

D

I

L

P

P

V

S

I

I

F

V

V

V

S

T

G

G

G

G

P

P

M

M

E

L

A

N

G

G

-

Y

-

F

-

R

-

G

-

E

K

R

V

V

G

G

D

S

K

G

A

T

-

H

L

L

D

W

L

K

V

F

D

S

S

E

M

M

V

V

M

K

A

A

A

G

D

E

D

-

S

-

V

M

S

T

D

Q

E

A

E

E

L

F

L

L

A