SitesBLAST
Comparing WP_079559050.1 NCBI__GCF_002201795.1:WP_079559050.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
47% identity, 99% coverage: 4:339/339 of query aligns to 68:393/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (= L116), R200 (= R145), M281 (≠ Y227), G282 (= G228), R307 (= R253), A308 (= A254), Q320 (= Q266), V321 (= V267), G322 (= G268), Q323 (= Q269), T324 (= T270), G337 (= G283), I338 (= I284), S339 (= S285), Q343 (= Q289), H344 (= H290), N358 (= N304), K359 (≠ N305), L377 (≠ V323)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
48% identity, 98% coverage: 1:331/339 of query aligns to 1:321/331 of 5ol2A
- binding calcium ion: E75 (≠ L75), D188 (= D199)
- binding flavin-adenine dinucleotide: T117 (= T117), R136 (= R145), I147 (= I156), G216 (= G226), R217 (≠ Y227), G218 (= G228), S242 (= S252), R243 (= R253), A244 (= A254), Q256 (= Q266), V257 (= V267), G258 (= G268), T260 (= T270), G273 (= G283), I274 (= I284), S275 (= S285), A277 (≠ Q287), Q279 (= Q289), H280 (= H290), N294 (= N304), K295 (≠ N305), D312 (= D322), V313 (= V323)
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
40% identity, 99% coverage: 3:337/339 of query aligns to 12:338/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (= L116), R144 (= R145), I155 (= I156), G224 (= G226), R225 (≠ Y227), G226 (= G228), S250 (= S252), R251 (= R253), A252 (= A254), Q264 (= Q266), V265 (= V267), G266 (= G268), Q267 (= Q269), S268 (≠ T270), G281 (= G283), I282 (= I284), S283 (= S285), S285 (≠ Q287), Q287 (= Q289), H288 (= H290), N302 (= N304), K303 (≠ N305), D320 (= D322), A321 (≠ V323)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
42% identity, 96% coverage: 4:330/339 of query aligns to 7:323/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (= T117), R140 (= R145), T142 (≠ A147), G219 (= G226), K220 (≠ Y227), G221 (= G228), S245 (= S252), R246 (= R253), A247 (= A254), Q259 (= Q266), V260 (= V267), G261 (= G268), Q262 (= Q269), T263 (= T270), G276 (= G283), S278 (= S285), Q282 (= Q289), H283 (= H290), N297 (= N304), I298 (≠ N305), L316 (≠ V323)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
37% identity, 94% coverage: 12:331/339 of query aligns to 21:328/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (= L116), T126 (= T117), R144 (= R145), I155 (= I156), R224 (≠ Y227), G225 (= G228), T249 (≠ S252), R250 (= R253), Q263 (= Q266), I264 (≠ V267), G265 (= G268), L266 (≠ Q269), S267 (≠ T270), G280 (= G283), I281 (= I284), S282 (= S285), Q286 (= Q289), N301 (= N304), S302 (≠ N305), D303 (= D306), D319 (= D322), L320 (≠ V323)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
37% identity, 71% coverage: 96:337/339 of query aligns to 111:333/333 of P13804
- G116 (= G101) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ G171) to I: decreased protein stability; dbSNP:rs1801591
- 205:333 (vs. 209:337, 52% identical) Domain II
- R223 (≠ Y227) binding FAD
- S248 (= S252) binding FAD
- R249 (= R253) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 267:270) binding FAD
- T266 (= T270) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (≠ SGQIQH 285:290) binding FAD
- N300 (= N304) binding FAD
- DL 318:319 (≠ DV 322:323) binding FAD
Sites not aligning to the query:
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
37% identity, 71% coverage: 96:336/339 of query aligns to 93:314/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G226), R205 (≠ Y227), S230 (= S252), R231 (= R253), A232 (= A254), Q244 (= Q266), V245 (= V267), G246 (= G268), T248 (= T270), G261 (= G283), I262 (= I284), S263 (= S285), A265 (≠ Q287), Q267 (= Q289), H268 (= H290), N282 (= N304), K283 (≠ N305), D300 (= D322), L301 (≠ V323)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
38% identity, 69% coverage: 97:330/339 of query aligns to 90:303/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G226), R200 (≠ Y227), G201 (= G228), S225 (= S252), R226 (= R253), A227 (= A254), Q239 (= Q266), V240 (= V267), G241 (= G268), T243 (= T270), G256 (= G283), I257 (= I284), S258 (= S285), A260 (≠ Q287), Q262 (= Q289), H263 (= H290), N277 (= N304), K278 (≠ N305), D295 (= D322), L296 (≠ V323)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
42% identity, 37% coverage: 211:335/339 of query aligns to 168:292/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G226), R184 (≠ Y227), G185 (= G228), S209 (= S252), R210 (= R253), Q223 (= Q266), I224 (≠ V267), G225 (= G268), T227 (= T270), G240 (= G283), V241 (≠ I284), S242 (= S285), A244 (≠ Q287), Q246 (= Q289), H247 (= H290), N261 (= N304), K262 (≠ N305), D279 (= D322), Y280 (≠ V323)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
28% identity, 96% coverage: 1:326/339 of query aligns to 1:311/321 of P53571
- M1 (= M1) modified: Initiator methionine, Removed
- R211 (≠ Y227) binding FAD
- SR 236:237 (= SR 252:253) binding FAD
- QVGQS 250:254 (≠ QVGQT 266:270) binding FAD
- 268:275 (vs. 283:290, 88% identical) binding FAD
- N289 (= N304) binding FAD
- DI 307:308 (≠ DV 322:323) binding FAD
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
27% identity, 96% coverage: 2:326/339 of query aligns to 1:310/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G226), R210 (≠ Y227), G211 (= G228), S235 (= S252), A236 (≠ R253), P237 (≠ A254), Q249 (= Q266), V250 (= V267), G251 (= G268), Q252 (= Q269), S253 (≠ T270), G267 (= G283), I268 (= I284), S269 (= S285), S271 (≠ Q287), Q273 (= Q289), H274 (= H290), N288 (= N304), T289 (≠ N305), D306 (= D322), I307 (≠ V323)
Query Sequence
>WP_079559050.1 NCBI__GCF_002201795.1:WP_079559050.1
MNNVFVICEMDEGKVADVSLELLTKGRTLANTLKCKLEAVVLGHKISGIEKEIIPYGADV
VHIGDDPKLSPYTTLPHSAVINGLFAETKPQIGLLGASTMGRDLGPRVSSALVSGLTADC
TALEIGDHEDKKAGKNYKDLLYQIRPAFGGNIVATIINPDCRPQMATVREGVMKKEIYSP
NHKGEVKTIDVSKYIKPEDLVVKVIERHMEKSRVNIKNSSIIVSGGYGMGSKENFNVLYD
LAEAIGGEVGASRAAVDAGYADHDRQVGQTGITVRPKLYIACGISGQIQHTAGMEDSAMV
IAINNDANAPINKIADYVITGDVMEIIPKMIKFYKKNSK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory