SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_079646907.1 NCBI__GCF_900167915.1:WP_079646907.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

3e5zA X-ray structure of the putative gluconolactonase in protein family pf08450. Northeast structural genomics consortium target drr130.
30% identity, 93% coverage: 13:300/310 of query aligns to 12:288/290 of 3e5zA

query
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binding magnesium ion: E30 (= E35), N117 (= N121), N174 (= N177), D220 (= D232)

3dr2A Structural and functional analyses of xc5397 from xanthomonas campestris: a gluconolactonase important in glucose secondary metabolic pathways (see paper)
36% identity, 85% coverage: 34:295/310 of query aligns to 44:298/299 of 3dr2A

query
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binding calcium ion: E45 (= E35), N131 (= N121), N188 (= N177), D236 (= D232)

7pldB Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
29% identity, 82% coverage: 35:289/310 of query aligns to 17:255/289 of 7pldB

query
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7pldB

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binding (R)-4-hydroxy-2-pyrrolidone: E17 (= E35), I31 (= I51), A64 (≠ R82), G66 (= G84), F67 (≠ R85), P80 (≠ K101), R98 (= R119), N100 (= N121), E119 (≠ P149), D138 (≠ V170), N145 (= N177), K180 (≠ A216), D195 (= D232), W210 (≠ P247), W210 (≠ P247)
binding fe (ii) ion: E17 (= E35), N145 (= N177), D195 (= D232)

Sites not aligning to the query:

binding (R)-4-hydroxy-2-pyrrolidone: 15

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
29% identity, 82% coverage: 35:289/310 of query aligns to 17:255/289 of 7plbB

query
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7plbB

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binding fe (ii) ion: E17 (= E35), N145 (= N177), D195 (= D232)
binding beta-D-xylopyranose: E17 (= E35), E89 (≠ D110), V90 (≠ K111), D92 (≠ R113), R98 (= R119), N100 (= N121), R109 (≠ A130), D130 (= D162), N145 (= N177), D174 (= D208), D195 (= D232)
binding alpha-D-xylopyranose: A64 (≠ R82), G66 (= G84), F67 (≠ R85), P80 (≠ K101)

Sites not aligning to the query:

binding beta-D-xylopyranose: 15

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
29% identity, 82% coverage: 35:289/310 of query aligns to 17:255/289 of Q9A9Z1

query
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Q9A9Z1

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D

N

V

S

A

G

G

S

V

L

Y

Y

R

R

V

M

S

-

P

-

D

D

L

L

Q

T

R

G

I

V

N

A

M

R

V

M

V

D

R

R

D

D

L

I

I

C

N

I

P

T

N
|
N

G

G

L

P

C

C

F

V

S

S

P

P

D

D

E

G

T

K

I

T

L

F

Y

Y

V

H

N

T

D

D

S

T

N

L

R

E

H

K

R

T

M

-

L

-

I

I

M

Y

A

A

Y

F

D

D

V

L

E

A

A

E

D

D

G

G

M

L

L

L

D

S

L

-

G

-

S

N

A

K

R

R

L

V

F

F

C

V

D

Q

M

F

R

A

-

L

G

G

D

D

R

V

P

Y

G

-

P

P

D
|
D

G

G

M

S

K

V

V

V

D

D

V

S

D

E

G

G

N

Y

V

L

Y

W

C

T

T

A

G

L

P

W

G

G

I

F

W

G

V

A

-

V

-

R

L

F

N

S

P

P

Q

Q

G

G

E

D

H

-

L

-

G

A

T

V

I

T

L

R

I

I

E

E

G

L

R

P

A

A

S

P

N

N

L

V

N

T

-

K

-

P

-

C

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

L

Y

Y

F

F

T

T

G

T

A

A

R

R

S

K

E17 (= E35) binding Fe(2+)
N145 (= N177) binding Fe(2+)
D195 (= D232) binding Fe(2+)

2dsoC Crystal structure of d138n mutant of drp35, a 35kda drug responsive protein from staphylococcus aureus (see paper)
27% identity, 73% coverage: 43:268/310 of query aligns to 52:271/323 of 2dsoC

query
sites
2dsoC

H

Q

G

G

H

Q

L

L

L

F

F

L

S

L

D

D

I

V

H

F

N

E

S

G

R

N

R

I

M

F

R

K

W

I

H

N

P

P

E

E

Q

T

G

K

I

-

S

E

I

I

D

K

K

R

-

P

-

F

-

V

E

S

D

H

T

K

Q

A

N

N

G

P

N

A

G

A

Q

I

T

K

L

I

D

H

R

K

E

D

G

G

R

R

L

L

V

F

V

V

C

C

H

Y

H

L

F

G

S

D

R

F

R

K

-
x
S

-
x
T

-
x
G

-

G

-

I

-

F

V

A

D

A

R

T

E

E

E

N

K

G

D

D

G

N

S

L

V

Q

T

D

V

I

A

E

D
|
D

K

L

Y

S

R
x
T

G
x
A

L
x
Y

K

C

L

I

N
|
N

R

-

P

-

N

-

D

D

V

M

V

V

V

F

K

D

S

S

D

K

G

G

A

G

I

F

Y

Y

F

F

T

T

D

D

P

-

K

-

V

-

P

-

F

F

T

R

P

G

I

Y

D

S

H

T

P

N

P

P

E

-

L

-

D

-

V

L

A

G

G

G

V

V

Y

Y

R

Y

V

V

S

S

P

P

D

D

L

F

Q

R

R

T

I

V

N

T

M

P

V

I

R

Q

D

N

L

I

I

S

N

V

P

A

N
|
N

G

G

L

I

C

A

F

L

S

S

P

T

D

D

E

E

T

K

I

V

L

L

Y

W

V

V

N

T

D

E

S

T

N

T

R

A

H

N

R

R

M

L

L

H

I

R

M

I

A

A

Y

-

D

-

V

L

E

E

A

D

D

D

G

G

M

V

-

T

L

I

D

Q

L

P

G

F

S

G

A

A

R

T

L

I

F

P

C

Y

D

Y

M

F

R

T

G

G

D

H

D

E

R

-

P

-

G

-

G

G

P

P

D
|
D

G
x
S

M

C

K

C

V

I

D

D

V

S

D

D

G

D

N

N

V

L

Y

Y

C

V

T

A

-

M

-

Y

G

G

P

Q

G

G

G

R

I

V

W

L

V

V

L

F

N

N

P

K

Q

R

G

G

E

Y

H

P

L

I

G

G

T

Q

I

I

L

L

I

I

E

P

G

G

R

R

binding calcium ion: S107 (vs. gap), T108 (vs. gap), G109 (vs. gap), D127 (= D110), T130 (≠ R113), A131 (≠ G114), Y132 (≠ L115), N135 (= N118), N182 (= N177), D233 (= D232), S234 (≠ G233)

Sites not aligning to the query:

binding calcium ion: 45

Q99QV3 Lactonase drp35; EC 3.1.1.- from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see paper)
27% identity, 73% coverage: 43:268/310 of query aligns to 54:273/324 of Q99QV3

query
sites
Q99QV3

H

Q

G

G

H

Q

L

L

L

F

F

L

S

L

D

D

I

V

H
x
F

N

E

S

G

R

N

R

I

M

F

R

K

W

I

H

N

P

P

E

E

Q

T

G

K

I

-

S

E

I

I

D

K

K

R

-

P

-

F

-

V

E

S

D

H

T

K

Q

A

N

N

G

P

N

A

G

A

Q

I

T
x
K

L

I

D

H

R

K

E

D

G

G

R

R

L

L

V

F

V

V

C

C

H

Y

H

L

F

G

S

D

R

F

R

K

-

S

-

T

-

G

-

I

-

F

V

A

D

A

R

T

E

E

E

N

K

G

D

D

G

N

S

L

V

Q

T

D

V

I

A

E

D

D

K

L

Y

S

R

T

G

A

L

Y

K

C

L

I

N
x
D

R

-

P

-

N

-

D
|
D

V

M

V

V

V

F

K

D

S

S

D

K

G

G

A

G

I

F

Y

Y

F

F

T

T

D
|
D

P

-

K

-

V

-

P

-

F

F

T

R

P

G

I

Y

D

S

H

T

P

N

P

P

E

-

L

-

D

-

V

L

A

G

G

G

V

V

Y

Y

R

Y

V

V

S

S

P

P

D

D

L

F

Q

R

R

T

I

V

N

T

M

P

V

I

R

Q

D

N

L

I

I

S

N

V

P

A

N
|
N

G
|
G

L

I

C

A

F

L

S

S

P

T

D

D

E

E

T

K

I

V

L

L

Y

W

V

V

N

T

D

E

S

T

N

T

R

A

H

N

R

R

M

L

L

H

I

R

M

I

A

A

Y

-

D

-

V

L

E

E

A

D

D

D

G

G

M

V

-

T

L

I

D

Q

L

P

G

F

S

G

A

A

R

T

L

I

F

P

C

Y

D

Y

M

F

R

T

G

G

D

H

D

E

R

-

P

-

G

-

G

G

P

P

D
|
D

G
x
S

M
x
C

K

C

V

I

D

D

V

S

D

D

G

D

N

N

V

L

Y

Y

C

V

T

A

-

M

-

Y

G

G

P

Q

G

G

G

R

I

V

W

L

V

V

L

F

N

N

P

K

Q

R

G

G

E

Y

H

P

L

I

G

G

T

Q

I

I

L

L

I

I

E

P

G

G

R

R

F63 (≠ H52) mutation to A: 2.5-fold increase in lactonase activity.
K92 (≠ T79) mutation to A: Increase in lactonase activity.
D137 (≠ N118) mutation to A: Loss of activity.; mutation to N: Loss of activity but still able to bind calcium ion.; mutation to S: Loss of activity.
D138 (= D122) mutation to A: Decrease in lactonase activity.
D151 (= D135) mutation to A: Decrease in lactonase activity.
N184 (= N177) mutation to A: Loss of activity.; mutation to D: Decrease in lactonase activity.; mutation to S: Loss of activity.
G185 (= G178) mutation to A: Decrease in lactonase activity.
D235 (= D232) mutation to A: Loss of activity.; mutation to N: Loss of activity due to inability to bind calcium ion.; mutation to S: Loss of activity.
S236 (≠ G233) mutation to A: Decrease in lactonase activity.
C237 (≠ M234) mutation to A: Increase in lactonase activity.

Sites not aligning to the query:

47 E→A: Loss of activity.; E→Q: Loss of activity due to inability to bind calcium ion.; E→S: Loss of activity.
280 R→A: Increase in lactonase activity.
301 E→A: Increase in lactonase activity.

7risA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound phosphate (see paper)
25% identity, 86% coverage: 35:302/310 of query aligns to 12:288/293 of 7risA

query
sites
7risA

E
|
E

G

G

P

P

V

V

W

-

I

V

A

M

E

P

H

D

G

G

H

S

L

V

F

L

S

V

D

E

I

I

H

R

N

A

S

Q

R

Q

R

L

M

T

R

R

W

V

H

W

P

P

E

D

Q

G

G

R

I

K

S

E

I

V

D

V

K

A

E

K

D

V

T

P

Q

G

N

G

G

P

N

N

G

G

Q

A

T

A

L

L

D

G

R

P

E

D

G

G

R

K

L

M

V

Y

V

I

C

C

H

N

F

G

S

G

-

F

-

G

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

-

S

-

I

R

Q

R

R

V

V

D

D

R

L

E

Q

E

S

K

-

D

-

G

G

S

E

V

V

T

E

V

T

V

L

A

F

D

D

K

K

Y

C

R

G

G

E

L

H

K

P

L

L

N

K

R

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

A

G

I

L

Y

W

F

F

T

T

D

D

P

L

P

G

P

K

K

R

V

-

P

-

F

-

T

-

P

-

I

-

D

-

H

R

P

A

P

R

E

D

L

M

D

D

V

V

A

G

G

A

V

A

Y

Y

R

Y

V

I

S

K

P

P

D

G

L

M

Q

T

R

E

I

I

N

T

M

E

V

Q

V

V

R

F

D

G

L

T

I

L

N

P

P

L

N
|
N

G

G

L

I

C

G

F

L

S

S

P

P

D

D

E

E

T

A

I

T

L

M

Y

Y

V

A

N

A

D

E

S

T

N

P

R

T

H

G

R

R

M

L

L

-

I

-

M

W

A

A

Y

F

D

D

V

L

E

S

A

G

D

P

G

G

M

E

L

V

-

K

-

P

-

R

D

D

L

G

G

E

S

K

A

G

R

K

L

P

F

I

C

C

D

G

M

L

R

G

G

G

D

Y

D

Q

R

M

P

-

G

-

P

F

D
|
D

G
x
S

M

L

K

A

V

V

D

E

V

A

D

S

G

G

N

N

V

V

Y

-

C

C

T

V

G

A

-

T

-

L

-

V

P

S

G

G

G

C

I

I

W

S

V

V

L

I

N

A

P

P

Q

D

G

G

E

T

H

L

L

V

G

E

T

Q

I

V

L

P

I

T

E

G

G

D

R

R

-

V

A

T

S
x
T

N

N

L

I

N

A

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

A

Y

Y

F

I

T

T

-

L

-

S

G

G

A

K

R

G

S

E

L

L

H

I

R

A

I

M

R

D

L

W

S

S

T

R

T

P

G

G

I

L

P

P

V

L

binding calcium ion: E12 (= E35), N111 (= N121), N160 (= N177), D213 (= D232), S214 (≠ G233)
binding phosphate ion: E12 (= E35), N111 (= N121), N160 (= N177), D213 (= D232), T254 (≠ S270)

8dk0A Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound (s)gamma- valerolactone (see paper)
24% identity, 86% coverage: 35:302/310 of query aligns to 14:288/293 of 8dk0A

query
sites
8dk0A

E
|
E

G

G

P

P

V

V

W

-

I

V

A

M

E

P

H

D

G

G

H

S

L

V

F

L

S

V

D

E

I

I

H

R

N

A

S

Q

R

Q

R

L

M

T

R

R

W

V

H

W

P

P

E

D

Q

G

G

R

I

K

S

E

I

V

D

V

K

A

E

K

D

V

T

P

Q

G

N

G

G

P

N

N

G

G

Q

A

T

A

L

L

D

G

R

P

E

D

G

G

R

K

L

M

V

Y

V

I

C

C

H

N

F

G

S

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

-

S

-

I

R

Q

R

R

V

V

D

D

R

L

E

Q

E

S

K

-

D

-

G

G

S

E

V

V

T

E

V

T

V

L

A

F

D

D

K

K

Y

C

R

G

G

E

L

H

K

P

L

L

N

K

R

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

A

G

I

L

Y

W

F

F

T

T

D

D

P

L

P

G

P

K

K

R

V

-

P

-

F

-

T

-

P

-

I

-

D

-

H

R

P

A

P

R

E

D

L

M

D

D

V

V

A

G

G

A

V

A

Y

Y

R

Y

V

I

S

K

P

P

D

G

L

M

Q

T

R

E

I

I

N

T

M

E

V

Q

V

V

R

F

D

G

L

T

I

L

N

P

P

L

N
|
N

G

G

L

I

C

G

F

L

S

S

P

P

D

D

E

E

T

A

I

T

L

M

Y

Y

V

A

N

A

D

E

S

T

N

P

R

T

H

G

R

R

M

L

L

-

I

-

M

W

A

A

Y

F

D

D

V

L

E

S

A

G

D

P

G

G

M

E

L

V

D

K

L

P

G

-

S

K

A

G

R

K

L

P

F

I

C

C

D

G

M

L

R

G

G

G

D

Y

D

Q

R

M

P

-

G

-

P

F

D
|
D

G
x
S

M

L

K

A

V

V

D

E

V

A

D

S

G

G

N

N

V

V

Y

-

C

C

T

V

G

A

-

T

-

L

-

V

P

S

G

G

G

C

I

I

W

S

V

V

L

I

N

A

P

P

Q

D

G

G

E

T

H

L

L

V

G

E

T

Q

I

V

L

P

I

T

E

G

G

D

R

R

-

V

A

T

S
x
T

N

N

L

I

N

A

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

A

Y

Y

F

I

T

T

-

L

-

S

G

G

A

K

R

G

S

E

L

L

H

I

R

A

I

M

R

D

L

W

S

S

T

R

T

P

G

G

I

L

P

P

V

L

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N177), D213 (= D232), S214 (≠ G233)
binding gamma-Valerolactone: E14 (= E35), F72 (vs. gap), D213 (= D232), T254 (≠ S270)

Sites not aligning to the query:

binding gamma-Valerolactone: 12

8djzA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound product (see paper)
24% identity, 86% coverage: 35:302/310 of query aligns to 14:288/293 of 8djzA

query
sites
8djzA

E
|
E

G

G

P

P

V

V

W

-

I

V

A

M

E

P

H

D

G

G

H

S

L

V

F

L

S

V

D

E

I

I

H

R

N

A

S

Q

R

Q

R

L

M

T

R

R

W

V

H

W

P

P

E

D

Q

G

G

R

I

K

S

E

I

V

D

V

K

A

E

K

D

V

T

P

Q

G

N

G

G

P

N

N

G

G

Q

A

T

A

L

L

D

G

R

P

E

D

G

G

R

K

L

M

V

Y

V

I

C

C

H

N

F

G

S

G

-

F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

-

S

-

I

R

Q

R

R

V

V

D

D

R

L

E

Q

E

S

K

-

D

-

G

G

S

E

V

V

T

E

V

T

V

L

A

F

D

D

K

K

Y

C

R

G

G

E

L

H

K

P

L

L

N

K

R

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

A

G

I

L

Y

W

F

F

T

T

D

D

P

L

P

G

P

K

K

R

V

-

P

-

F

-

T

-

P

-

I

-

D

-

H

R

P

A

P

R

E

D

L

M

D

D

V

V

A

G

G

A

V

A

Y

Y

R

Y

V

I

S

K

P

P

D

G

L

M

Q

T

R

E

I

I

N

T

M

E

V

Q

V

V

R

F

D

G

L

T

I

L

N

P

P

L

N
|
N

G

G

L

I

C

G

F

L

S

S

P

P

D

D

E

E

T

A

I

T

L

M

Y

Y

V

A

N

A

D

E

S

T

N

P

R

T

H

G

R

R

M

L

L

-

I

-

M

W

A

A

Y

F

D

D

V

L

E

S

A

G

D

P

G

G

M

E

L

V

D

K

L

P

G

-

S

K

A

G

R

K

L

P

F

I

C

C

D

G

M

L

R

G

G

G

D

Y

D

Q

R

M

P

-

G

-

P

F

D
|
D

G
x
S

M

L

K

A

V

V

D

E

V

A

D

S

G

G

N

N

V

V

Y

-

C

C

T

V

G

A

-

T

-

L

-

V

P

S

G

G

G

C

I

I

W

S

V

V

L

I

N

A

P

P

Q

D

G

G

E

T

H

L

L

V

G

E

T

Q

I

V

L

P

I

T

E

G

G

D

R

R

-

V

A

T

S

T

N

N

L

I

N

A

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

A

Y

Y

F

I

T

T

-

L

-

S

G

G

A

K

R

G

S

E

L

L

H

I

R

A

I

M

R

D

L

W

S

S

T

R

T

P

G

G

I

L

P

P

V

L

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N177), D213 (= D232), S214 (≠ G233)
binding (4R)-4-hydroxypentanoic acid: E14 (= E35), N115 (= N121), D213 (= D232)

8djfA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound tetrahedral intermediate (see paper)
24% identity, 86% coverage: 35:302/310 of query aligns to 14:288/293 of 8djfA

query
sites
8djfA

E
|
E

G

G

P

P

V

V

W

-

I

V

A

M

E

P

H

D

G

G

H

S

L

V

F

L

S

V

D

E

I

I

H

R

N

A

S

Q

R

Q

R

L

M

T

R

R

W

V

H

W

P

P

E

D

Q

G

G

R

I

K

S

E

I

V

D

V

K

A

E

K

D

V

T

P

Q

G

N

G

G

P

N
|
N

G

G

Q

A

T

A

L

L

D

G

R

P

E

D

G

G

R

K

L

M

V

Y

V

I

C

C

H

N

F

G

S

G

-
x
F

-

G

-

W

-

M

-

P

-

G

-

A

-

P

-

A

-

P

-

H

-

E

-

Y

-

I

-

G

-

S

-

I

R

Q

R

R

V

V

D

D

R

L

E

Q

E

S

K

-

D

-

G

G

S

E

V

V

T

E

V

T

V

L

A

F

D

D

K

K

Y

C

R

G

G

E

L

H

K

P

L

L

N

K

R

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

A

G

I

L

Y

W

F

F

T

T

D

D

P

L

P

G

P

K

K

R

V

-

P

-

F

-

T

-

P

-

I

-

D

-

H

R

P

A

P

R

E

D

L

M

D

D

V

V

A

G

G

A

V

A

Y

Y

R

Y

V

I

S

K

P

P

D

G

L

M

Q

T

R

E

I

I

N

T

M

E

V

Q

V

V

R

F

D

G

L

T

I

L

N

P

P

L

N
|
N

G

G

L

I

C

G

F

L

S

S

P

P

D

D

E

E

T

A

I

T

L

M

Y

Y

V

A

N

A

D

E

S

T

N

P

R

T

H

G

R

R

M

L

L

-

I

-

M

W

A

A

Y

F

D

D

V

L

E

S

A

G

D

P

G

G

M

E

L

V

D

K

L

P

G

-

S

K

A

G

R

K

L

P

F

I

C

C

D

G

M

L

R

G

G

G

D

Y

D

Q

R

M

P

-

G

-

P

F

D
|
D

G
x
S

M

L

K

A

V

V

D

E

V

A

D

S

G

G

N

N

V

V

Y

-

C

C

T

V

G

A

-

T

-

L

-

V

P

S

G

G

G

C

I

I

W

S

V

V

L

I

N

A

P

P

Q

D

G

G

E

T

H

L

L

V

G

E

T

Q

I

V

L

P

I

T

E

G

G

D

R

R

-

V

A

T

S

T

N

N

L

I

N

A

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

A

Y

Y

F

I

T

T

-

L

-

S

G

G

A

K

R

G

S

E

L

L

H

I

R

A

I

M

R

D

L

W

S

S

T

R

T

P

G

G

I

L

P

P

V

L

binding calcium ion: E14 (= E35), N115 (= N121), N164 (= N177), D213 (= D232), S214 (≠ G233)
binding (5S)-5-methyloxolane-2,2-diol: E14 (= E35), N54 (= N76), F72 (vs. gap), N115 (= N121), D213 (= D232)

7rizA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound 2-hydroxyquinoline (see paper)
23% identity, 86% coverage: 35:302/310 of query aligns to 12:301/306 of 7rizA

query
sites
7rizA

E
|
E

G

G

P

P

V

V

W

-

I

V

A

M

E

P

H

D

G

G

H

S

L

V

F

L

S

V

D

E

I

I

H

R

N

A

S

Q

R

Q

R

L

M

T

R

R

W

V

H

W

P

P

E

D

Q

G

G

R

I

K

S

E

I

V

D

V

K

A

E

K

D

V

T

P

Q

G

N

G

G

P

N
|
N

G

G

Q

A

T

A

L

L

D

G

R

P

E

D

G

G

R

K

L

M

V

Y

V

I

C

C

-

N

-

G

-
x
F

-

G

-

W

-

F

-

P

-

S

-

R

-

G

-

T

-

M

-
x
P

-

G

-

A

-

P

-

A

-

P

H

H

H

E

F

Y

S

I

-

G

-

S

-

I

R

Q

R

R

V

V

D

D

R

L

E

Q

E

S

K

-

D

-

G

G

S

E

V

V

T

E

V

T

V

L

A

F

D

D

K

K

Y

C

R

G

G

E

L

H

K

P

L

L

N

K

R

G

P

P

N
|
N

D

D

V

L

V

V

V

F

K

D

S

K

D

H

G

G

A

G

I

L

Y

W

F

F

T

T

D

D

P

L

P

G

P

K

K

R

V

-

P

-

F

-

T

-

P

-

I

-

D

-

H

R

P

A

P

R

E

D

L

M

D

D

V

V

A

G

G

A

V

A

Y

Y

R

Y

V

I

S

K

P

P

D

G

L

M

Q

T

R

E

I

I

N

T

M

E

V

Q

V

V

R

F

D

G

L

T

I

L

N

P

P

L

N
|
N

G

G

L

I

C

G

F

L

S

S

P

P

D

D

E

E

T

A

I

T

L

M

Y

Y

V

A

N

A

D

E

S

T

N

P

R

T

H

G

R

R

M

L

L

-

I

-

M

W

A

A

Y

F

D

D

V

L

E

S

A

G

D

P

G

G

-

E

-

V

-

K

-

P

-

R

-

D

-

V

-

I

-

Y

-

R

-

G

-

E

-

K

-

G

-

K

-

P

M

I

L

C

D

G

L

L

G

G

S

G

A

Y

R

Q

L

M

F

F

C

-

D

-

M

-

R

-

G

-

D

-

R

-

P

-

G

-

P

-

D
|
D

G
x
S

M

L

K

A

V

V

D

E

V

A

D

S

G

G

N

N

V

V

Y

-

C

C

T

V

G

A

-

T

-

L

-

V

P

S

G

G

G

C

I

I

W

S

V

V

L

I

N

A

P

P

Q

D

G

G

E

T

H

L

L

V

G

E

T

Q

I

V

L

P

I

T

E

G

G

D

R

R

-

V

A

T

S

T

N

N

L

I

N

A

W

F

G

G

D

G

A

P

D

D

W

L

R

K

T

T

L

A

Y

Y

F

I

T

T

-

L

-

S

G

G

A

K

R

G

S

E

L

L

H

I

R

A

I

M

R

D

L

W

S

S

T

R

T

P

G

G

I

L

P

P

V

L

binding calcium ion: E12 (= E35), N120 (= N121), N169 (= N177), D226 (= D232), S227 (≠ G233)
binding quinolin-2(1h)-one: E12 (= E35), N52 (= N76), F70 (vs. gap), P81 (vs. gap), N120 (= N121), D226 (= D232)

Q2Q1W2 E3 ubiquitin-protein ligase TRIM71; Protein lin-41 homolog; RING-type E3 ubiquitin transferase TRIM71; Tripartite motif-containing protein 71; EC 2.3.2.27 from Homo sapiens (Human) (see paper)
29% identity, 36% coverage: 23:135/310 of query aligns to 743:859/868 of Q2Q1W2

query
sites
Q2Q1W2

L

L

G

W

R

K

G

H

F

F

G

D

G

S

P

P

T
x
R

G

G

P

-

A

-

E

-

G

-

P

-

V

V

W

A

I

F

A

N

E

H

H

E

G

G

H

H

L

L

L

V

F

V

S

T

D

D

I

F

H

N

N

N

S

H

R

R

R

L

M

L

R

V

W

I

H

H

P

P

-

D

-

C

E

Q

Q

S

G

A

I

R

S

F

I

L

D

G

K

S

E

E

D

G

T

T

Q

G

N

N

G

G

N

Q

-

F

-

L

-

R

-

P

-

Q

G

G

Q

V

T

A

L

V

D

D

R

Q

E

E

G

G

R

R

L

I

V

I

V

V

C

A

H

D

H

S

F

R

S

N

R

H

R

R

V

V

D

Q

R

M

E

F

E

E

K

S

D

N

G

G

S

S

-

F

V

L

T

C

V

K

V

F

A

G

D

A

K

Q

Y

G

R

S

G

G

L

F

-

G

K

Q

L

M

N

D

R

R

P

P

N

S

D

G

V

I

V

A

V

I

K

T

S

P

D

D

G

G

A

M

I

I

Y

V

F

V

T

V

D

D

R751 (≠ T31) mutation to A: Decreases repression on mRNA. Abolishes interaction with AGO2, HSP90AA1, PABPC1 and PUM2.

Sites not aligning to the query:

647 G→D: Strongly decreases repression on mRNA.
675 H→L: Abolishes repression on mRNA. Abolishes interaction with AGO2, PABPC1 and PUM2. Increases interaction with HSP90AA1.
702 Y→A: Decreases repression on mRNA. Decreases interaction with HSP90AA1. Abolishes interaction with PABPC1 and PUM2. Increases interaction with AGO2.

Q1PSW8 E3 ubiquitin-protein ligase TRIM71; Protein lin-41 homolog; mLin41; RING-type E3 ubiquitin transferase TRIM71; Tripartite motif-containing protein 71; EC 2.3.2.27 from Mus musculus (Mouse) (see paper)
30% identity, 36% coverage: 23:135/310 of query aligns to 730:846/855 of Q1PSW8

query
sites
Q1PSW8

L

L

G

W

R

K

G

H

F

F

G

D

G

S

P

P

T

R

G

G

P

-

A

-

E

-

G

-

P

-

V

V

W

A

I

F

A

N

E

N

H

E

G

G

H

H

L

L

L

V

F

V

S

T

D

D

I

F

H

N

N

N

S

H

R

R

R

L

M

L

R

V

W

I

H

H

P

P

E

D

Q

C

G

Q

I

S

S

A

I

R

D

F

K

L

E

G

D

S

T

E

Q

G

N

S

G

G

N

N

G

G

Q

Q

T

F

L

L

-

R

-

P

-

Q

-

G

-

V

-

A

-

V

D

D

R

Q

E

E

G

G

R

R

L

I

V

I

V

V

C

A

H

D

H

S

F

R

S

N

R

H

R

R

V

V

D

Q

R

M

E

F

E

E

K

A

D

N

G

G

S

S

-

F

V

L

T

C

V

K

V

F

A

G

D

A

K

Q

Y

G

R

S

G

G

L

F

-

G

K

Q

L

M

N

D

R

R

P

P

N

S

D

G

V

I

V

A

V

V

K

T

S

P

D

D

G

G

A

L

I

I

Y

V

F

V

T

V

D

D

Sites not aligning to the query:

12 C→L: Abolishes E3 ubiquitin-protein ligase activity; when associated with A-15.
15 C→A: Abolishes E3 ubiquitin-protein ligase activity; when associated with L-12.

Query Sequence

>WP_079646907.1 NCBI__GCF_900167915.1:WP_079646907.1
MPIERLSAALDAIIPPGTDSEELGRGFGGPTGPAEGPVWIAEHGHLLFSDIHNSRRMRWH
PEQGISIDKEDTQNGNGQTLDREGRLVVCHHFSRRVDREEKDGSVTVVADKYRGLKLNRP
NDVVVKSDGAIYFTDPPPKVPFTPIDHPPELDVAGVYRVSPDLQRINMVVRDLINPNGLC
FSPDETILYVNDSNRHRMLIMAYDVEADGMLDLGSARLFCDMRGDDRPGGPDGMKVDVDG
NVYCTGPGGIWVLNPQGEHLGTILIEGRASNLNWGDADWRTLYFTGARSLHRIRLSTTGI
PVPRGALPTS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory