SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_079648441.1 NCBI__GCF_900167915.1:WP_079648441.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
55% identity, 98% coverage: 4:196/196 of query aligns to 3:195/195 of P48636

query
sites
P48636

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E74 (= E75) binding substrate
M89 (= M90) mutation to A: Almost complete loss of activity.
R92 (= R93) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 93:94) binding substrate
109:115 (vs. 110:116, 71% identical) binding substrate
T112 (= T113) mutation to A: Almost complete loss of activity.
E114 (= E115) mutation to A: Almost complete loss of activity.
E115 (= E116) mutation to A: Almost complete loss of activity.
E118 (= E119) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
58% identity, 88% coverage: 4:176/196 of query aligns to 2:174/182 of 5zbkA

query
sites
5zbkA

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binding adenosine monophosphate: A11 (≠ S13), E73 (= E75), M88 (= M90), R91 (= R93), K92 (= K94), G108 (= G110), G110 (= G112), T111 (= T113)

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
48% identity, 90% coverage: 4:180/196 of query aligns to 2:177/181 of 5zblD

query
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binding adenosine monophosphate: S11 (= S13), M47 (= M49), R90 (= R93), K91 (= K94), G107 (= G110), G109 (= G112), T110 (= T113), E112 (= E115), E113 (= E116)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
46% identity, 91% coverage: 4:182/196 of query aligns to 35:213/242 of Q5ZC82

query
sites
Q5ZC82

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G73 (= G42) mutation to D: In LOG-4; decreased activity.

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
40% identity, 92% coverage: 3:183/196 of query aligns to 1:204/217 of 5ajuA

query
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5ajuA

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binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S13), R108 (= R93), G131 (= G110), Y132 (≠ I111), G133 (= G112), T134 (= T113), E137 (= E116)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 85% coverage: 6:171/196 of query aligns to 12:176/187 of O05306

query
sites
O05306

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M54 (= M49) mutation to A: Large decrease in catalytic activity.
K74 (= K69) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E75) mutation to A: Large decrease in catalytic activity.
R98 (= R93) mutation to A: Loss of catalytic activity.
T118 (= T113) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 115:116) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
42% identity, 85% coverage: 6:171/196 of query aligns to 8:172/183 of 7w2iA

query
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7w2iA

A

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L

L

H

I

I

V

V

T

G

D

S

T

M

M

H

W

E

E

R

R

K

K

A

Q

M

I

M

M

A

E

D

D

R

R

S

S

D

D

G

A

F

F

I

I

A

V

L

L

S

P

G

G

I

V

G

G

T

T

F

L

E

D

E

E

L

L

F

F

E
x
D

I

A

W

W

T

T

W
x
D

G

G

Q

Y

L

L

G

G

D

T

H

H

A

D

K

K

P

P

V

I

A

V

L

M

F

V

N

D

V

P

A

W

G

G

F

H

Y

F

D

D

K

G

L

L

A

R

G

A

F

W

L

L

D

N

D

G

V

L

T

D

A

T

G

G

F

Y

L

V

R

S

P

P

A

T

H

A

R

M

A

E

M

R

L

L

M

V

V

V

D

V

D

D

D

N

binding magnesium ion: D120 (≠ E119), D124 (≠ W123)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
41% identity, 67% coverage: 39:170/196 of query aligns to 35:167/177 of 3sbxA

query
sites
3sbxA

L

L

V

V

Y

W

G

G

R

H

V

V

G

S

L

A

M

M

G

G

V

A

V

V

A

S

D

S

S

A

V

A

L

R

A

A

A

H

G

G

R

W

A

T

T

V

G

G

V

V

I

I

P

P

K

K

A

M

L

L

A

V

D

H

L

R

E
|
E

V

L

A

A

H

D

L

H

G

D

L

A

T

D

E

E

L

L

H

V

I

V

V

T

G

E

S

T

M
|
M

H

W

E

E

R
|
R

K
|
K

A

Q

M

V

M

M

A

E

D

D

R

R

S

A

D

N

G

A

F

F

I

I

A

T

L

L

S

P

G

G

G
|
G

I

V

G
|
G

T
|
T

F

L

E
x
D

E
|
E

L

L

F

L

E

D

I

V

W

W

T

T

W

E

G

G

Q

Y

L

L

G

G

D

M

H

H

A

D

K

K

P

S

V

I

A

V

L

V

F

L

N

D

V

P

A

W

G

G

F

H

Y

F

D

D

K

G

L

L

A

R

G

A

F

W

L

L

D

S

D

E

V

L

V

A

T

D

A

T

G

G

F

Y

L

V

-

S

R

R

P

T

A

A

H

M

R

E

A

R

M

L

L

I

M

V

V

V

D

D

D

N

binding adenosine monophosphate: E71 (= E75), M86 (= M90), R89 (= R93), K90 (= K94), G106 (= G110), G108 (= G112), T109 (= T113), D111 (≠ E115), E112 (= E116)

Query Sequence

>WP_079648441.1 NCBI__GCF_900167915.1:WP_079648441.1
MNPIRALCVFCGSSPGVDPVHAAAARALGIALAEAGVDLVYGGGRVGLMGVVADSVLAAG
GRATGVIPKALADLEVAHLGLTELHIVGSMHERKAMMADRSDGFIALSGGIGTFEELFEI
WTWGQLGDHAKPVALFNVAGFYDKLAGFLDDVVTAGFLRPAHRAMLMVDDDPAALVRRMR
DHRAPLVEKWIDRAER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory