SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_079649071.1 NCBI__GCF_900167915.1:WP_079649071.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
51% identity, 94% coverage: 2:134/141 of query aligns to 44:176/176 of Q96PE7

query
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Q96PE7

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H50 (= H8) binding Co(2+)
R104 (≠ E63) to L: in dbSNP:rs6748672
H122 (= H81) binding Co(2+)
E172 (= E130) binding Co(2+)

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
52% identity, 91% coverage: 3:131/141 of query aligns to 9:135/138 of 6qh4C

query
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6qh4C

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active site: H14 (= H8), V50 (= V45), E61 (= E56), H86 (= H81), E134 (= E130)
binding cobalt (ii) ion: H14 (= H8), H86 (= H81), E134 (= E130)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
39% identity, 94% coverage: 2:134/141 of query aligns to 1:132/138 of 3oa4A

query
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3oa4A

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active site: H7 (= H8), I43 (≠ V45), E54 (= E56), H78 (= H81), E128 (= E130)
binding zinc ion: H7 (= H8), E54 (= E56), H78 (= H81), E128 (= E130)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
38% identity, 94% coverage: 3:135/141 of query aligns to 2:139/141 of 6wf6A

query
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6wf6A

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active site: H7 (= H8), E43 (≠ V45), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E56), H84 (= H81), E134 (= E130)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
38% identity, 94% coverage: 3:135/141 of query aligns to 2:139/139 of 6wfhA

query
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active site: H7 (= H8), E43 (≠ V45), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H8), Q39 (= Q41), Q60 (≠ E56), A70 (≠ P66), K73 (≠ G69), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (≠ R122), L132 (= L128), E134 (= E130)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
38% identity, 94% coverage: 2:134/141 of query aligns to 1:138/144 of 6xbqA

query
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6xbqA

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active site: H7 (= H8), E43 (≠ V45), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding carboxymethyldethia coenzyme *a: Q39 (= Q41), Q60 (≠ E56), A70 (≠ P66), K73 (≠ G69), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), F122 (= F118), P125 (= P121), K126 (≠ R122), L132 (= L128)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E56), H84 (= H81), E134 (= E130)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
38% identity, 94% coverage: 2:134/141 of query aligns to 1:138/144 of 6wfiA

query
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6wfiA

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active site: H7 (= H8), E43 (≠ V45), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q41), Q60 (≠ E56), A70 (≠ P66), K73 (≠ G69), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), G114 (= G110), S115 (≠ A111), I120 (= I116), F122 (= F118), P125 (= P121), L132 (= L128), E134 (= E130)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
38% identity, 94% coverage: 2:134/141 of query aligns to 1:138/144 of 6wf7A

query
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6wf7A

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I

V

A

G

G

K

F
x
W

L

L

A

A

K

K

N

N

P

G

D

E

G

G

G

-

Q

V

H
|
H

H
|
H

I

I

C

A

Y

F

E

G

V

T

P

A

D

D

I

V

H

D

A

A

A

D

K

A

A

A

W

D

F

I

E

R

G

D

K

K

G

G

A

V

R

R

V

V

L
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L

-

Y

G

D

E

E

P

P

R

R

I

R

G
|
G

A
x
S

H

M

G

G

T

S

L

R

I
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I

F

T

F
|
F

V

L

H

H

P
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P

R
x
K

D

D

M

C

G

H

G

G

V

V

L
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L

T

T

E
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E

I

L

M

V

E

T

T

S

active site: H7 (= H8), E43 (≠ V45), Q60 (≠ E56), H84 (= H81), E134 (= E130)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (= Q41), E43 (≠ V45), Q60 (≠ E56), A70 (≠ P66), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (≠ R122), L132 (= L128), E134 (= E130)
binding methylmalonyl-coenzyme a: Q39 (= Q41), Q60 (≠ E56), A70 (≠ P66), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), G114 (= G110), S115 (≠ A111), I120 (= I116), F122 (= F118), P125 (= P121), K126 (≠ R122), L132 (= L128), E134 (= E130)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
38% identity, 95% coverage: 2:135/141 of query aligns to 1:139/140 of 6xbtA

query
sites
6xbtA

K

S

L

L

G

T

R

R

L

I

N

D

H
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H

V

I

G

G

V

I

A

A

T

C

P

H

S

D

I

L

E

D

K

A

S

T

I

V

A

E

F

F

Y

Y

R

R

D

A

V

T

M

Y

G

G

A

F

E

E

V

V

I

F

R

H

E

T

P

E

F

V

D

N

L

-

P

E

A

E

Q
|
Q

G

G

V

V

K

R

V
x
E

C

A

F

M

V

L

-

K

-

I

-

N

D

D

T

T

P

S

N

D

-

G

-

G

-

A

S

S

Q

Y

I

L

E
x
A

L

L

I

L

E

E

P

P

L

T

G

R

E

E

S

D

S

S

P
x
A

I

V

A

G

G
x
K

F
x
W

L

L

A

A

K
|
K

N

N

P

G

D

E

G

G

G

-

Q

V

H
|
H

H
|
H

I

I

C

A

Y

F

E

G

V

T

P

A

D

D

I

V

H

D

A

A

A

D

K

A

A

A

W

D

F

I

E

R

G

D

K

K

G

G

A

V

R

R

V

V

L
|
L

-

Y

G

D

E

E

P

P

R

R

I

R

G
|
G

A
x
S

H

M

G

G

T

S

L

R

I

I

F

T

F
|
F

V

L

H

H

P
|
P

R
x
K

D

D

M

C

G

H

G

G

V

V

L
|
L

T

T

E
|
E

I

L

M

V

E

T

T

S

P

A

active site: H7 (= H8), E43 (≠ V45), A60 (≠ E56), H84 (= H81), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), H84 (= H81), E134 (= E130)
binding coenzyme a: Q39 (= Q41), A70 (≠ P66), K73 (≠ G69), W74 (≠ F70), K77 (= K73), H83 (= H80), L107 (= L104), F122 (= F118), P125 (= P121), K126 (≠ R122), L132 (= L128)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q41), A70 (≠ P66), K73 (≠ G69), W74 (≠ F70), H83 (= H80), H84 (= H81), L107 (= L104), G114 (= G110), S115 (≠ A111), P125 (= P121), K126 (≠ R122), L132 (= L128), E134 (= E130)

2qh0A Crystal structure of a glyoxalase from clostridium acetobutylicum
27% identity, 91% coverage: 5:133/141 of query aligns to 3:128/129 of 2qh0A

query
sites
2qh0A

R

K

L

V

N

H

H
|
H

V

I

G

G

V

Y

A

A

T

V

P

K

S

N

I

I

E

D

K

S

S

A

I

L

A

K

F

K

Y

F

R

K

D

R

-

L

-

G

-

Y

V

V

M

E

G

E

A

S

E

E

V

V

I

V

R

R

E

D

P

E

F

-

D

-

L

-

P

-

A

V

Q

R

G

K

V

V

K

Y

V

I

C

Q

F

F

V

V

D

I

T

N

P

G

N

G

S

Y

Q

R

I

V

E
|
E

L

L

I

V

E

A

P

P

L

D

G

G

E

E

S

D

S

S

P

P

I

I

A

N

G

K

F

T

L

I

A

K

K

K

N

G

P

-

D

-

G

S

G

T

Q

P

H

Y

H
|
H

I

I

C

C

Y

Y

E

E

V

V

P

E

D

D

I

I

H

Q

A

K

A

S

K

I

A

E

W

E

F

M

E

S

G

Q

K

I

G

G

A

Y

R

T

V

L

L

F

G

K

E

K

P

A

R

E

I

I

-

A

-

P

G

A

A

I

H

D

G

N

T

R

L

K

I

V

F

A

F

F

V

L

H

F

P

S

R

T

D

D

M

I

G

G

G

-

V

-

L

L

T

I

E
|
E

I

L

M

L

E

E

binding zinc ion: H6 (= H8), E53 (= E56), H76 (= H81), E125 (= E130)

Query Sequence

>WP_079649071.1 NCBI__GCF_900167915.1:WP_079649071.1
MKLGRLNHVGVATPSIEKSIAFYRDVMGAEVIREPFDLPAQGVKVCFVDTPNSQIELIEP
LGESSPIAGFLAKNPDGGQHHICYEVPDIHAAKAWFEGKGARVLGEPRIGAHGTLIFFVH
PRDMGGVLTEIMETPRPGVSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory