SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_079649347.1 NCBI__GCF_900167915.1:WP_079649347.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9WYP7 5-keto-L-gluconate epimerase; Bifunctional nonphosphorylated sugar isomerase; D-erythrose/D-threose isomerase; L-ribulose 3-epimerase; R3E; Nonphosphorylated sugar 3-epimerase; Nonphosphorylated sugar aldose-ketose isomerase; EC 5.1.3.-; EC 5.1.3.-; EC 5.3.1.- from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
37% identity, 47% coverage: 130:270/299 of query aligns to 109:245/270 of Q9WYP7

query
sites
Q9WYP7

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E149 (= E174) binding Mn(2+)
E155 (= E180) binding substrate
D182 (= D207) binding Mn(2+)
H185 (= H210) binding substrate
H208 (≠ Q232) binding Mn(2+)
R214 (= R239) binding substrate
E243 (= E268) binding Mn(2+)

5b80A Crystal structure of hyperthermophilic thermotoga maritima l-ketose-3- epimerase with cu2+ (see paper)
37% identity, 47% coverage: 130:270/299 of query aligns to 109:245/269 of 5b80A

query
sites
5b80A

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binding copper (ii) ion: E149 (= E174), D182 (= D207), H208 (≠ Q232), E243 (= E268)

5b7yA Crystal structure of hyperthermophilic thermotoga maritima l-ketose-3- epimerase with co2+ (see paper)
37% identity, 47% coverage: 130:270/299 of query aligns to 109:245/269 of 5b7yA

query
sites
5b7yA

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binding cobalt (ii) ion: E149 (= E174), D182 (= D207), H208 (≠ Q232), E243 (= E268)

5h1wA Crystal structure of hyperthermophilic thermotoga maritima l-ketose-3- epimerase with mn2+ and l(+)-erythrulose (see paper)
37% identity, 47% coverage: 130:270/299 of query aligns to 110:246/270 of 5h1wA

query
sites
5h1wA

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binding L-Erythrulose: E150 (= E174), L152 (= L176), E156 (= E180), H186 (= H210), H209 (≠ Q232), R215 (= R239), E244 (= E268)
binding manganese (ii) ion: E150 (= E174), D183 (= D207), H209 (≠ Q232), E244 (= E268)

3kwsA Crystal structure of putative sugar isomerase (yp_001305149.1) from parabacteroides distasonis atcc 8503 at 1.68 a resolution
29% identity, 46% coverage: 156:292/299 of query aligns to 122:265/265 of 3kwsA

query
sites
3kwsA

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binding magnesium ion: E143 (= E174), D176 (= D207), H202 (≠ Q232), E240 (= E268)

3vylA Structure of l-ribulose 3-epimerase (see paper)
36% identity, 40% coverage: 159:277/299 of query aligns to 132:250/297 of 3vylA

query
sites
3vylA

R

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E

binding manganese (ii) ion: E147 (= E174), D180 (= D207), H206 (≠ Q232), E241 (= E268)

7cj9D Crystal structure of n-terminal his-tagged d-allulose 3-epimerase from methylomonas sp. With additional c-terminal residues (see paper)
33% identity, 40% coverage: 158:276/299 of query aligns to 136:254/289 of 7cj9D

query
sites
7cj9D

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binding D-fructose: E152 (= E174), E158 (= E180), H188 (= H210), H211 (≠ Q232), R217 (= R239), E246 (= E268)
binding manganese (ii) ion: E152 (= E174), D185 (= D207), H211 (≠ Q232), E246 (= E268)

Sites not aligning to the query:

binding D-fructose: 12, 69, 261

A0A172U6X0 L-ribulose 3-epimerase; LRE; MetLRE; EC 5.1.3.31 from Methylomonas sp. (strain DH-1) (see paper)
33% identity, 40% coverage: 158:276/299 of query aligns to 136:254/286 of A0A172U6X0

query
sites
A0A172U6X0

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E152 (= E174) binding D-allulose; binding D-fructose; binding Mn(2+)
E158 (= E180) binding D-allulose; binding D-fructose
D185 (= D207) binding Mn(2+)
H188 (= H210) binding D-allulose; binding D-fructose
H211 (≠ Q232) binding D-allulose; binding D-fructose; binding Mn(2+)
R217 (= R239) binding D-allulose; binding D-fructose
E246 (= E268) binding D-allulose; binding D-fructose; binding Mn(2+)

Sites not aligning to the query:

9 Y→I: Affects thermal stability. Slight increase of the activity with D-allulose as substrate; when associated with F-37 and L-286.
12 binding D-allulose; binding D-fructose
37 Y→F: Affects thermal stability. Slight increase of the activity with D-allulose as substrate; when associated with Y-9 and L-286.
69 binding D-allulose; binding D-fructose
286 Y→L: Affects thermal stability. Slight increase of the activity with D-allulose as substrate; when associated with Y-9 and F-37.

7cj7B Crystal structure of homo dimeric d-allulose 3-epimerase from methylomonas sp. In complex with l-tagatose (see paper)
34% identity, 39% coverage: 158:274/299 of query aligns to 135:251/286 of 7cj7B

query
sites
7cj7B

K

Q

R

R

L

L

G

A

P

D

I

Y

A

A

Q

A

A

D

H

K

G

G

V

I

I

N

L

I

A

D

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E
|
E

T

T

N
|
N

F
x
I

I

M

N

N

R

T

V

G

S

L

E
|
E

G

G

G

L

A

A

I

F

V

L

R

D

A

E

V

V

G

N

H

R

P

P

S

N

V

A

G

F

L

L

V

H

A

L

D
|
D

L

T

F

Y

H
|
H

M

M

R

N

M

I

E

E

D

E

E

N

G

G

P

M

A

A

-

K

S

S

L

V

I

L

E

A

N

A

G

G

P

D

L

R

I

L

R

G

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R
|
R

R

G

A

Y

P

L

G

G

V

T

A

G

G

N

E

V

D

D

F

F

S

A

A

S

Y

F

F

F

E

A

A

A

L

L

R

K

S

Q

I

I

G

D

Y

Y

A

R

R

G

R

P

L

I

S

T

V

F

E
|
E

C

S

L

F

W

S

S

S

D

E

I

I

binding (2R,3R,4R,5S)-2-(hydroxymethyl)oxane-2,3,4,5-tetrol: N159 (= N182), I160 (≠ F183), E166 (= E189)
binding L-tagatose: E151 (= E174), E157 (= E180), H187 (= H210), H210 (≠ Q232), R216 (= R239), E245 (= E268)
binding manganese (ii) ion: E151 (= E174), D184 (= D207), H210 (≠ Q232), E245 (= E268)

Sites not aligning to the query:

7cj7A Crystal structure of homo dimeric d-allulose 3-epimerase from methylomonas sp. In complex with l-tagatose (see paper)
34% identity, 39% coverage: 158:274/299 of query aligns to 135:251/286 of 7cj7A

query
sites
7cj7A

K

Q

R

R

L

L

G

A

P

D

I

Y

A

A

Q

A

A

D

H

K

G

G

V

I

I

N

L

I

A

D

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E
|
E

T

T

N

N

F

I

I

M

N

N

R

T

V

G

S

L

E

E

G

G

G

L

A

A

I

F

V

L

R

D

A

E

V

V

G

N

H

R

P

P

S

N

V

A

G

F

L

L

V

H

A

L

D
|
D

L

T

F

Y

H
|
H

M

M

R

N

M

I

E

E

D

E

E

N

G

G

P

M

A

A

-

K

S

S

L

V

I

L

E

A

N

A

G

G

P

D

L

R

I

L

R

G

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R
|
R

R

G

A

Y

P

L

G

G

V

T

A

G

G

N

E

V

D

D

F

F

S

A

A

S

Y

F

F

F

E

A

A

A

L

L

R

K

S

Q

I

I

G

D

Y

Y

A

R

R

G

R

P

L

I

S

T

V

F

E
|
E

C

S

L

F

W

S

S

S

D

E

I

I

binding manganese (ii) ion: E151 (= E174), D184 (= D207), H210 (≠ Q232), E245 (= E268)
binding L-sorbose: E151 (= E174), E157 (= E180), H187 (= H210), H210 (≠ Q232), R216 (= R239), E245 (= E268)

Sites not aligning to the query:

binding L-sorbose: 11, 68, 69, 260

7cj6A Crystal structure of homo dimeric d-allulose 3-epimerase from methylomonas sp. In complex with d-allulose (see paper)
34% identity, 39% coverage: 158:274/299 of query aligns to 135:251/286 of 7cj6A

query
sites
7cj6A

K

Q

R

R

L

L

G

A

P

D

I

Y

A

A

Q

A

A

D

H

K

G

G

V

I

I

N

L

I

A

D

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E
|
E

T

T

N

N

F

I

I

M

N

N

R

T

V

G

S

L

E

E

G

G

G

L

A

A

I

F

V

L

R

D

A

E

V

V

G

N

H

R

P

P

S

N

V

A

G

F

L

L

V

H

A

L

D
|
D

L

T

F

Y

H
|
H

M

M

R

N

M

I

E

E

D

E

E

N

G

G

P

M

A

A

-

K

S

S

L

V

I

L

E

A

N

A

G

G

P

D

L

R

I

L

R

G

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R
|
R

R

G

A

Y

P

L

G

G

V

T

A

G

G

N

E

V

D

D

F

F

S

A

A

S

Y

F

F

F

E

A

A

A

L

L

R

K

S

Q

I

I

G

D

Y

Y

A

R

R

G

R

P

L

I

S

T

V

F

E
|
E

C

S

L

F

W

S

S

S

D

E

I

I

binding manganese (ii) ion: E151 (= E174), D184 (= D207), H210 (≠ Q232), E245 (= E268)
binding D-psicose: E151 (= E174), E157 (= E180), H187 (= H210), H210 (≠ Q232), R216 (= R239), E245 (= E268)

Sites not aligning to the query:

binding D-psicose: 11, 68, 260

7cj5A Crystal structure of homo dimeric d-allulose 3-epimerase from methylomonas sp. In complex with d-fructose (see paper)
34% identity, 39% coverage: 158:274/299 of query aligns to 135:251/292 of 7cj5A

query
sites
7cj5A

K

Q

R

R

L

L

G

A

P

D

I

Y

A

A

Q

A

A

D

H

K

G

G

V

I

I

N

L

I

A

D

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E
|
E

T

T

N

N

F

I

I

M

N

N

R

T

V

G

S

L

E

E

G

G

G

L

A

A

I

F

V

L

R

D

A

E

V

V

G

N

H

R

P

P

S

N

V

A

G

F

L

L

V

H

A

L

D
|
D

L

T

F

Y

H
|
H

M

M

R

N

M

I

E

E

D

E

E

N

G

G

P

M

A

A

-

K

S

S

L

V

I

L

E

A

N

A

G

G

P

D

L

R

I

L

R

G

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R
|
R

R

G

A

Y

P

L

G

G

V

T

A

G

G

N

E

V

D

D

F

F

S

A

A

S

Y

F

F

F

E

A

A

A

L

L

R

K

S

Q

I

I

G

D

Y

Y

A

R

R

G

R

P

L

I

S

T

V

F

E
|
E

C

S

L

F

W

S

S

S

D

E

I

I

binding D-fructose: E151 (= E174), E157 (= E180), H187 (= H210), H210 (≠ Q232), R216 (= R239), E245 (= E268)
binding manganese (ii) ion: E151 (= E174), D184 (= D207), H210 (≠ Q232), E245 (= E268)

Sites not aligning to the query:

binding beta-D-fructopyranose: 8, 37
binding D-fructose: 11, 68, 69, 260

6wn6B Crystal structure of 3-keto-d-glucoside 4-epimerase, ycjr, from e. Coli, apo form (see paper)
27% identity, 69% coverage: 55:261/299 of query aligns to 28:235/264 of 6wn6B

query
sites
6wn6B

G

G

F

F

V

D

E

G

E

F

E

E

V

I

D

D

R

G

F

K

L

L

P

V

D

N

K

N

P

-

D

-

A

-

F

I

E

E

A

E

A

V

R

K

A

A

A

I

R

K

A

E

A

T

P

G

I

L

P

P

V

V

R

T

T

T

L

A

V

C

R

G

L

G

L

Y

P

D

P

G

S

W

L

I

Q

G

S

D

V

F

G

I

P

E

D

E

T

R

A

R

H

L

D

N

G

G

I

L

V

K

T

Q

F

I

C

E

E

R

T

I

V

L

F

E

A

A

R

L

A

A

R

E

R

V

A

G

G

G

V

K

E

G

T

I

V

V

-

P

-

A

F

W

G

G

S

M

P

F

R

T

S

F

R

R

R

L

I

P

P

P

E

M

G

T

F

S

P

P

R

R

A

S

T

L

A

D

-

G

-

D

Q

R

A

K

Q

M

L

V

I

S

D

D

L

S

C

L

K

R

R

V

L

L

G

E

P

Q

I

V

A

A

Q

A

A

R

H

T

G

G

V

T

I

V

L

V

A

Y

L

L

E
|
E

P

P

L

L

N

N

H

R

T

Y

E

Q

T

D

N

H

F

M

I

I

N

N

R

T

V

L

S

A

E

D

G

A

G

R

A

R

I

Y

V

I

R

V

A

E

V

N

G

D

H

L

P

K

S

H

V
|
V

G

Q

L

I

V

I

A

G

D
|
D

L

F

F

Y

H

H

M

M

R

N

M

I

E

E

D

E

E

D

G

N

P

L

A

A

-

Q

S

A

L

L

I

H

E

D

N

N

G

R

P

D

L

L

I

L

R

G

H

H

V

V

Q
x
H

I

I

A

A

E

D

K

N

Q

H

G

-

R

R

R

Y

A

Q

P

P

G

G

V

S

A

G

G

T

E
x
L

D

D

F

F

S

H

A

A

Y
x
L

F
|
F

E

E

A

Q

L

L

R

R

S

A

I

D

G

N

Y

Y

active site: V176 (= V202), L221 (≠ E247), L226 (≠ Y252), F227 (= F253)
binding manganese (ii) ion: E148 (= E174), D181 (= D207), H207 (≠ Q232)

Sites not aligning to the query:

binding manganese (ii) ion: 242

7ernC Crystal structure of d-allulose 3-epimerase with d-fructose from agrobacterium sp. Sul3
28% identity, 67% coverage: 94:294/299 of query aligns to 66:260/284 of 7ernC

query
sites
7ernC

L

V

L

L

P

P

-

E

P

P

S

A

L

W

Q

A

S

S

V

V

G

R

P

P

D

E

T

A

A

A

H

-

D

-

G

-

I

-

V

V

T

A

F

H

C

L

E

N

T

A

V

A

F

L

A

D

R

K

A

A

R

A

R

E

A

M

G

G

V

A

E

E

T

A

-

L

-

T

-

G

V

V

V

T

F

Y

G

G

S

G

P

T

R

S

S

E

R

R

R

T

I

-

P

-

E

-

G

G

F

F

P

P

R

-

A

-

T

P

A

T

Q

Q

A

A

Q

E

L

Y

I

D

D

N

L

L

C

T

K

R

R

A

L

L

G

S

P

Q

I

S

A

A

-

G

-

H

-

A

Q

K

A

T

H

L

G

G

V

L

I

Q

L

F

A

G

L

I

E
|
E

P

A

L

V

N

N

H

R

T

Y

E
|
E

T

N

N

H

F

L

I

V

N

N

R

S

V

A

S

E

E

Q

G

A

G

V

A

A

I

L

V

V

R

E

A

R

V

I

G

G

H

A

P

D

S

N

V

I

G

F

L

V

V

H

A

L

D
|
D

L

T

F

F

H
|
H

M

M

R

N

M

M

E

E

D

E

E

K

G

G

P

I

A

A

S

N

-

G

L

I

I

I

E

A

N

A

G

H

P

D

L

Y

I

L

R

K

H

Y

V

M

Q
x
H

I

M

A

S

E

E

K

S

Q

D

G

-

R
|
R

R

G

A

T

P

P

G

G

V

F

A

G

G

N

E

V

D

A

F

W

S

D

A

A

Y

V

F

F

E

A

A

A

L

L

R

A

S

A

I

I

G

G

Y

F

A

K

R

G

R

V

L

L

S

T

V

L

E
|
E

C

S

L

F

W

-

S

A

D

A

I

M

A

P

Q

E

E

E

A

M

P

A

V

G

A

A

T

I

R

S

T

T

I

W

R

R

A

-

Q

-

Y

-

P

P

A

V

A

A

A

S

G

G

binding D-fructose: E144 (= E174), E150 (= E180), H180 (= H210), H203 (≠ Q232), R209 (= R239), E238 (= E268)
binding magnesium ion: E144 (= E174), D177 (= D207), H203 (≠ Q232), E238 (= E268)

Sites not aligning to the query:

binding D-fructose: 64

7eroA Crystal structure of d-allulose 3-epimerase with d-allulose from agrobacterium sp. Sul3
28% identity, 67% coverage: 94:294/299 of query aligns to 66:260/283 of 7eroA

query
sites
7eroA

L

V

L

L

P

P

-

E

P

P

S

A

L

W

Q

A

S

S

V

V

G

R

P

P

D

E

T

A

A

A

H

-

D

-

G

-

I

-

V

V

T

A

F

H

C

L

E

N

T

A

V

A

F

L

A

D

R

K

A

A

R

A

R

E

A

M

G

G

V

A

E

E

T

A

-

L

-

T

-

G

V

V

V

T

F

Y

G

G

S

G

P

T

R

S

S

E

R

R

R

T

I

-

P

-

E

-

G

G

F

F

P

P

R

-

A

-

T

P

A

T

Q

Q

A

A

Q

E

L

Y

I

D

D

N

L

L

C

T

K

R

R

A

L

L

G

S

P

Q

I

S

A

A

-

G

-

H

-

A

Q

K

A

T

H

L

G

G

V

L

I

Q

L

F

A

G

L

I

E
|
E

P

A

L

V

N

N

H

R

T

Y

E
|
E

T

N

N

H

F

L

I

V

N

N

R

S

V

A

S

E

E

Q

G

A

G

V

A

A

I

L

V

V

R

E

A

R

V

I

G

G

H

A

P

D

S

N

V

I

G

F

L

V

V

H

A

L

D
|
D

L

T

F

F

H
|
H

M

M

R

N

M

M

E

E

D

E

E

K

G

G

P

I

A

A

S

N

-

G

L

I

I

I

E

A

N

A

G

H

P

D

L

Y

I

L

R

K

H

Y

V

M

Q
x
H

I

M

A

S

E

E

K

S

Q

D

G

-

R
|
R

R

G

A

T

P

P

G

G

V

F

A

G

G

N

E

V

D

A

F

W

S

D

A

A

Y

V

F

F

E

A

A

A

L

L

R

A

S

A

I

I

G

G

Y

F

A

K

R

G

R

V

L

L

S

T

V

L

E
|
E

C

S

L

F

W

-

S

A

D

A

I

M

A

P

Q

E

E

E

A

M

P

A

V

G

A

A

T

I

R

S

T

T

I

W

R

R

A

-

Q

-

Y

-

P

P

A

V

A

A

A

S

G

G

binding magnesium ion: E144 (= E174), D177 (= D207), H203 (≠ Q232), E238 (= E268)
binding D-psicose: E144 (= E174), E150 (= E180), H180 (= H210), H203 (≠ Q232), R209 (= R239), E238 (= E268)

Sites not aligning to the query:

binding D-psicose: 64

8xivA Production of d-psicose
28% identity, 50% coverage: 112:261/299 of query aligns to 87:238/290 of 8xivA

query
sites
8xivA

V

I

T

T

F

F

C

V

E

K

T

K

V

V

F

M

A

D

R

A

A

M

R

H

R

Q

A

A

G

G

V

I

E

H

-

R

-

I

-

G

T

T

V

V

V

Y

F

S

G

Y

S

W

P

P

R

V

S

D

R

Y

R

S

I

G

P

P

E

-

G

-

F

F

P

D

R

K

A

P

T

A

A

A

Q

R

A

K

Q

H

L

S

I

I

D

E

L

S

C

V

K

R

R

E

L

L

G

A

P

E

I

Y

A

A

Q

R

A

Q

H

Y

G

N

V

I

I

T

L

L

A

L

L

I

E
|
E

P

T

L

L

N

N

H

R

T

F

E
|
E

T

Q

N

F

F

L

I

L

N

N

R

D

V

A

S

E

E

E

G

A

G

V

A

A

I

Y

V

V

R

K

A

E

V

V

G

N

H

K

P

P

S

N

V

V

G

K

L

V

V

M

A

L

D
|
D

L

T

F

F

H
|
H

M

M

R

N

M

I

E

E

D

E

E

D

G

H

P

I

A

A

S

D

L

A

I

I

E

R

-

Y

N

T

G

G

P

D

L

H

I

L

R

G

H

Q

V

L

Q
x
H

I

I

A

G

E

E

K

-

Q

A

G

N

R
|
R

R

K

A

V

P

P

G

G

V

K

A

G

G

S

E

M

D

P

F

W

S

T

A

E

Y

I

F

G

E

Q

A

A

L

L

R

K

S

D

I

I

G

R

Y

Y

binding cobalt (ii) ion: E151 (= E174), D184 (= D207), H210 (≠ Q232)
binding D-fructose: E151 (= E174), E157 (= E180), H187 (= H210), H210 (≠ Q232), R216 (= R239)

Sites not aligning to the query:

2hk1A Crystal structure of d-psicose 3-epimerase (dpease) in the presence of d-fructose (see paper)
28% identity, 45% coverage: 146:281/299 of query aligns to 122:270/289 of 2hk1A

query
sites
2hk1A

R

K

A

A

T

G

A

D

Q

Y

A

A

Q

R

L

G

I

V

D

E

L

G

C

I

K

N

R

G

L

I

G

A

P

D

I

F

A

A

Q

N

A

D

H

L

G

G

V

I

I

N

L

L

A

C

L

I

E
|
E

P

V

L

L

N

N

H

R

T

F

E
|
E

T

N

N

H

F

V

I

L

N

N

R

T

V

A

S

A

E

E

G

G

G

V

A

A

I

F

V

V

R

K

A

D

V

V

G

G

H

K

P

N

S

N

V

V

G

K

L

V

V

M

A

L

D
|
D

L

T

F

F

H
|
H

M

M

R

N

M

I

E

E

D

E

E

D

G

S

P

F

A

G

S

D

L

A

I

I

E

R

N

T

-

A

G

G

P

P

L

L

I

L

R

G

H

H

V

F

Q
x
H

I

T

A

G

E

E

K

S

Q

N

G

-

R
|
R

R

R

A

V

P

P

G

G

V

K

A

G

G

R

E

M

D

P

F

W

S

H

A

E

Y

I

F

G

E

L

A

A

L

L

R

R

S

D

I

I

G

N

Y

Y

A

T

R

G

R

A

L

V

S

I

V

M

E
|
E

-

P

-

F

-

V

-

K

-

T

-

G

-

T

-

I

-

G

-

S

-

D

-

I

C

K

L

V

W

W

S

R

D

D

I

L

A

S

Q

G

E

G

A

A

P

D

V

I

A

A

binding D-fructose: E150 (= E174), E156 (= E180), H186 (= H210), H209 (≠ Q232), R215 (= R239), E244 (= E268)
binding manganese (ii) ion: E150 (= E174), D183 (= D207), H209 (≠ Q232), E244 (= E268)

Sites not aligning to the query:

binding D-fructose: 66, 67

A9CH28 D-psicose 3-epimerase; DPEase; EC 5.1.3.30 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
28% identity, 45% coverage: 146:281/299 of query aligns to 122:270/289 of A9CH28

query
sites
A9CH28

R

K

A

A

T

G

A

D

Q

Y

A

A

Q

R

L

G

I

V

D

E

L

G

C

I

K

N

R

G

L

I

G

A

P

D

I

F

A

A

Q

N

A

D

H

L

G

G

V

I

I

N

L

L

A

C

L

I

E
|
E

P

V

L

L

N

N

H

R

T

F

E
|
E

T

N

N

H

F

V

I

L

N

N

R

T

V

A

S

A

E

E

G

G

G

V

A

A

I

F

V

V

R

K

A

D

V

V

G

G

H

K

P

N

S

N

V

V

G

K

L

V

V

M

A

L

D
|
D

L
x
T

F
|
F

H
|
H

M

M

R

N

M

I

E

E

D

E

E

D

G

S

P

F

A

G

S

D

L

A

I

I

E

R

N

T

-

A

G

G

P

P

L

L

I

L

R

G

H

H

V

F

Q
x
H

I

T

A

G

E

E

K

S

Q

N

G

-

R
|
R

R

R

A

V

P

P

G

G

V

K

A

G

G

R

E

M

D

P

F

W

S

H

A

E

Y

I

F

G

E

L

A

A

L

L

R

R

S

D

I

I

G

N

Y

Y

A

T

R

G

R

A

L

V

S

I

V

M

E
|
E

-

P

-

F

-

V

-

K

-

T

-

G

-

T

-

I

-

G

-

S

-

D

-

I

C

K

L

V

W

W

S

R

D

D

I

L

A

S

Q

G

E

G

A

A

P

D

V

I

A

A

E150 (= E174) binding Mn(2+)
E156 (= E180) binding substrate
D183 (= D207) binding Mn(2+)
DTFH 183:186 (≠ DLFH 207:210) binding substrate
H209 (≠ Q232) binding Mn(2+)
R215 (= R239) binding substrate
E244 (= E268) binding Mn(2+)

Sites not aligning to the query:

107 binding substrate

8yecC Crystal structure of l-ribulose 3-epimerase in complex with d-allulose
29% identity, 42% coverage: 159:285/299 of query aligns to 139:263/297 of 8yecC

query
sites
8yecC

R

R

L

V

G

A

P

D

I

R

A

A

Q

S

A

D

H

L

G

G

V

I

I

N

L

V

A

S

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E
|
E

T

T

N

N

F

V

I

L

N

N

R

T

V

G

S

R

E

Q

G

A

G

L

A

A

I

Y

V

L

R

E

A

E

V

L

G

N

H

R

P

P

S

N

V

L

G

G

L

I

V

H

A

L

D
|
D

L

T

F

Y

H
|
H

M

M

R

N

M

I

E

E

D

E

E

S

G

D

P

M

A

F

S

S

-

P

L

I

I

L

E

D

N

T

G

A

P

E

L

A

I

L

R

R

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R
|
R

R

G

A

Y

P

L

G

G

V

T

A

G

G

S

E

V

D

D

F

F

S

D

A

T

Y

F

F

F

E

K

A

A

L

L

R

G

S

R

I

I

G

G

Y

Y

A

D

R

G

R

P

L

V

S

V

V

F

E
|
E

C

S

L

F

W

S

S

S

D

S

I

V

A

V

Q

-

E

-

A

A

P

P

V

D

A

L

T

S

R

R

T

M

I

L

binding D-fructose: E154 (= E174), E160 (= E180), H190 (= H210), H213 (≠ Q232), R219 (= R239), E248 (= E268)
binding magnesium ion: E154 (= E174), D187 (= D207), H213 (≠ Q232), E248 (= E268)
binding D-psicose: E154 (= E174), E160 (= E180), H190 (= H210), H213 (≠ Q232), R219 (= R239), E248 (= E268)

Sites not aligning to the query:

binding D-fructose: 71, 72
binding D-psicose: 14, 71, 118

A0A1L7NQ96 Ketose 3-epimerase; D-allulose 3-epimerase; D-AE; L-ribulose 3-epimerase; EC 5.1.3.- from Arthrobacter globiformis (see paper)
29% identity, 42% coverage: 159:285/299 of query aligns to 131:255/289 of A0A1L7NQ96

query
sites
A0A1L7NQ96

R

R

L

V

G

A

P

D

I

R

A

A

Q

S

A

D

H

L

G

G

V

I

I

N

L

V

A

S

L

L

E
|
E

P

V

L

V

N

N

H

R

T

Y

E

E

T

T

N

N

F

V

I

L

N

N

R

T

V

G

S

R

E

Q

G

A

G

L

A

A

I

Y

V

L

R

E

A

E

V

L

G

N

H

R

P

P

S

N

V

L

G

G

L

I

V

H

A

L

D
|
D

L

T

F

Y

H

H

M

M

R

N

M

I

E

E

D

E

E

S

G

D

P

M

A

F

S

S

-

P

L

I

I

L

E

D

N

T

G

A

P

E

L

A

I

L

R

R

H

Y

V

V

Q
x
H

I

I

A

G

E

E

K

S

Q

H

G

-

R

R

R

G

A

Y

P

L

G

G

V

T

A

G

G

S

E

V

D

D

F

F

S

D

A

T

Y

F

F

F

E

K

A

A

L

L

R

G

S

R

I

I

G

G

Y

Y

A

D

R

G

R

P

L

V

S

V

V

F

E
|
E

C

S

L

F

W

S

S

S

D

S

I

V

A

V

Q

-

E

-

A

A

P

P

V

D

A

L

T

S

R

R

T

M

I

L

E146 (= E174) binding Mn(2+)
D179 (= D207) binding Mn(2+)
H205 (≠ Q232) binding Mn(2+)
E240 (= E268) binding Mn(2+)

Query Sequence

>WP_079649347.1 NCBI__GCF_900167915.1:WP_079649347.1
MTAAALDRRRFLAGTAACLAAGIAGAKTPAAPALPSLGAWTSLDKAALLAASGYGFVEEE
VDRFLLPDKPDAAFEAARAAARAAPIPVRTLVRLLPPSLQSVGPDTAHDGIVTFCETVFA
RARRAGVETVVFGSPRSRRIPEGFPRATAQAQLIDLCKRLGPIAQAHGVILALEPLNHTE
TNFINRVSEGGAIVRAVGHPSVGLVADLFHMRMEDEGPASLIENGPLIRHVQIAEKQGRR
APGVAGEDFSAYFEALRSIGYARRLSVECLWSDIAQEAPVATRTIRAQYPAAAGKAARG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory