SitesBLAST
Comparing WP_081433906.1 NCBI__GCF_000010525.1:WP_081433906.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 66% coverage: 94:385/440 of query aligns to 63:376/446 of A0A0H2VG78
- R102 (= R141) mutation to A: Loss of transport activity.
- I105 (≠ Q144) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E161) mutation to A: Loss of transport activity.
- Q137 (≠ V176) mutation to A: Loss of transport activity.
- Q250 (≠ E264) mutation to A: Loss of transport activity.
- Q251 (≠ G265) mutation to A: Loss of transport activity.
- N256 (≠ L270) mutation to A: Loss of transport activity.
- W357 (≠ P366) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 22 D→N: Affects symport activity. May function as an uniporter.
Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
26% identity, 39% coverage: 46:216/440 of query aligns to 56:207/444 of Q8NLB7
- D57 (= D47) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- R103 (= R99) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
- 309 W→V: Loss of transport activity.
- 312 D→A: Loss of transport activity.
- 313 R→A: Loss of transport activity.
- 317 mutation I->H,Y: Loss of transport activity.
- 386 R→A: Loss of transport activity.
Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
24% identity, 52% coverage: 28:256/440 of query aligns to 36:274/583 of Q9Y7Q9
- S267 (≠ A249) modified: Phosphoserine
- S269 (≠ G251) modified: Phosphoserine
Sites not aligning to the query:
- 289 modified: Phosphoserine
- 290 modified: Phosphoserine
- 292 modified: Phosphoserine
- 330 modified: Phosphoserine
9g11A Sugar/h+ symporter stp6 in inward occluded conformation with glucose bound
24% identity, 73% coverage: 38:357/440 of query aligns to 13:348/476 of 9g11A
Sites not aligning to the query:
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
35% identity, 28% coverage: 94:217/440 of query aligns to 78:185/452 of Q5EXK5
- D82 (= D98) mutation to A: Loss of activity.
Sites not aligning to the query:
- 311 V→W: Loss of activity.
- 314 D→A: Loss of activity.
O42885 Putative inorganic phosphate transporter C8E4.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 42% coverage: 4:189/440 of query aligns to 20:209/572 of O42885
Sites not aligning to the query:
- 12 modified: Phosphoserine
- 14 modified: Phosphoserine
- 292 modified: Phosphoserine
- 296 modified: Phosphoserine
Q9C757 Probable inositol transporter 2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 53% coverage: 97:330/440 of query aligns to 92:353/580 of Q9C757
Sites not aligning to the query:
- 399 C→A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413.; C→S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410.
- 402 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413.
- 410 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413.; C→S: No effect on inostol transport or nickel inhibition; when associated with S-399.
- 413 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410.
P38695 Probable glucose transporter HXT5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 55% coverage: 88:331/440 of query aligns to 149:421/592 of P38695
Sites not aligning to the query:
- 61 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q09852 Putative inorganic phosphate transporter C23D3.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 25% coverage: 80:189/440 of query aligns to 95:204/559 of Q09852
Sites not aligning to the query:
- 297 modified: Phosphoserine
- 299 modified: Phosphoserine
P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
28% identity, 31% coverage: 88:225/440 of query aligns to 117:267/587 of P25297
- C145 (≠ S116) mutation to S: No significant effect on transport activity; when associated with S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- F160 (≠ P134) mutation to C: Reduces phosphate binding, transport and signaling activities.
- R168 (= R141) mutation to A: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to C: Abolishes transport and signaling activities.; mutation to E: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- G172 (= G145) mutation to C: Abolishes transport and signaling activities.
- G174 (≠ S147) mutation to C: Abolishes transport and signaling activities.
- G176 (= G149) mutation to C: Abolishes transport and signaling activities.
- D178 (≠ E151) mutation D->E,N: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates.
- Y179 (≠ Q152) mutation to A: Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity.; mutation to G: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity.; mutation Y->S,F: No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity.
- C237 (≠ V193) mutation to S: No significant effect on transport activity; when associated with S-145, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C241 (≠ L197) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C245 (≠ A203) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C263 (≠ A221) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
Sites not aligning to the query:
- 6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
- 298 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); K→A: No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization.
- 304:327 mutation Missing: Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition.
- 335 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-399, S-434, S-455, S-474, S-510 and S-519.
- 358 D→E: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.; D→N: Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- 392 V→C: Reduces transport and signaling activities.
- 399 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-434, S-455, S-474, S-510 and S-519.
- 408 V→C: Reduces signaling activity and enhances transport activity.
- 434 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-455, S-474, S-510 and S-519.
- 455 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-474, S-510 and S-519.
- 473 E→K: No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; E→Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.
- 474 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-510 and S-519.
- 492 mutation K->A,Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.; K→E: Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- 510 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-519.
- 519 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-510.
Q9LT15 Sugar transport protein 10; AtSTP10; D-glucose-H(+) symport protein STP10; D-glucose-proton symporter STP10; Hexose transporter 10 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 50% coverage: 38:258/440 of query aligns to 33:246/514 of Q9LT15
- F39 (≠ E44) mutation to A: Reduces affinity for glucose 8-fold.
- L43 (≠ F48) mutation to A: Reduces affinity for glucose 150-fold and turns STP10 into a low affinity transporter.
- C77 (≠ I70) modified: Disulfide link with 449; mutation to A: Increases sensitivity to alkaline pH and can only function fully at acidic pH (pH < 5).
- E162 (= E161) mutation to Q: Abolishes glucose transport activity; when associated with N-344.
- Q177 (≠ L184) binding beta-D-glucose; mutation to A: Reduces affinity for glucose 37-fold.
- I184 (≠ A191) mutation to A: Reduces affinity for glucose 3-fold.
Sites not aligning to the query:
- 295 binding beta-D-glucose
- 296 binding beta-D-glucose
- 301 binding beta-D-glucose
- 332 binding beta-D-glucose
- 344 D→N: Abolishes glucose transport activity; when associated with Q-162.
- 410 binding beta-D-glucose
- 449 modified: Disulfide link with 77; C→A: Increases sensitivity to alkaline pH and can only function fully at acidic pH (pH < 5).
7aaqA Sugar/h+ symporter stp10 in outward occluded conformation (see paper)
26% identity, 50% coverage: 38:258/440 of query aligns to 13:226/487 of 7aaqA
Sites not aligning to the query:
Q8IVW8 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2 from Homo sapiens (Human) (see 2 papers)
27% identity, 47% coverage: 38:245/440 of query aligns to 109:305/549 of Q8IVW8
- R200 (= R141) mutation to S: Loss of function; does not rescue the cardia bifida phenotype in the morpholino knockdown in zebrafish.
Sites not aligning to the query:
- 319 natural variant: Missing (in DFNB115; uncertain significance; dbSNP:rs749994718)
8fvzA Pipt y150a
25% identity, 43% coverage: 29:217/440 of query aligns to 1:190/433 of 8fvzA
Sites not aligning to the query:
Query Sequence
>WP_081433906.1 NCBI__GCF_000010525.1:WP_081433906.1
MRWDKASADFADDGSGEGAVSVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFAR
NFFPDSSAFIGLLSAFGMFAASFLMRPLGGILFGRVGDRLGRRRALFLSAMMMTLSTVSI
GLLPTAASAGAMAPVLLLALRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGAT
LGTLLGSAVGASVASLLPPQDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPK
APLREAMRAHGRQILHAMLIVVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVV
VLIASPAAALLSDRIGRKKVLLAALVGLILCAWPVFRLLSSDNLPQMIAGQVILALLASC
YGGPMPAALCELFQRRTRCTAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVIV
LSCVSLAGVLAMTDKSVGPI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory