SitesBLAST
Comparing WP_082109398.1 NCBI__GCF_001305595.1:WP_082109398.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P20932 (S)-mandelate dehydrogenase; MDH; L(+)-mandelate dehydrogenase; EC 1.1.99.31 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
38% identity, 91% coverage: 11:387/415 of query aligns to 8:379/393 of P20932
- PTG 79:81 (= PTG 82:84) binding FMN
- G81 (= G84) mutation to A: 23-fold decrease in catalytic activity with mandelate as substrate and DCPIP as electron acceptor, but no change in affinity for mandelate. Shows a modestly higher reactivity with molecular oxygen.
- S108 (= S111) binding FMN
- Q129 (= Q132) binding FMN
- T156 (= T160) binding FMN
- K250 (= K256) binding FMN
- DSGFR 303:307 (≠ DSGIR 311:315) binding FMN
- GR 326:327 (= GR 334:335) binding FMN
6bfgA Crystal structure of monotopic membrane protein (s)-mandelate dehydrogenase (see paper)
38% identity, 90% coverage: 11:382/415 of query aligns to 6:372/373 of 6bfgA
- active site: Y129 (= Y134), D156 (= D162), H272 (= H280)
- binding flavin mononucleotide: Y24 (= Y29), P77 (= P82), T78 (= T83), G79 (= G84), Q127 (= Q132), Y129 (= Y134), T154 (= T160), K248 (= K256), H272 (= H280), G273 (= G281), R275 (= R283), D301 (= D311), S302 (= S312), G303 (= G313), R305 (= R315), G324 (= G334), R325 (= R335)
6gmcA 1.2 a resolution structure of human hydroxyacid oxidase 1 bound with fmn and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
36% identity, 90% coverage: 6:380/415 of query aligns to 3:358/360 of 6gmcA
- active site: Y132 (= Y134), D160 (= D162), H258 (= H280)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y26 (= Y29), M82 (≠ L85), W110 (≠ V113), Y132 (= Y134), R167 (= R169), F191 (≠ T193), L203 (= L225), Y206 (≠ W228), H258 (= H280), R261 (= R283)
- binding flavin mononucleotide: Y26 (= Y29), Y27 (≠ V30), A79 (≠ P82), T80 (= T83), A81 (≠ G84), S108 (= S111), Q130 (= Q132), Y132 (= Y134), T158 (= T160), K234 (= K256), H258 (= H280), G259 (= G281), R261 (= R283), D289 (= D311), G290 (≠ S312), R293 (= R315), G312 (= G334), R313 (= R335)
Q9UJM8 2-Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GO; GOX; Glyoxylate oxidase; EC 1.1.3.15; EC 1.2.3.5 from Homo sapiens (Human) (see 2 papers)
36% identity, 90% coverage: 6:380/415 of query aligns to 3:360/370 of Q9UJM8
- Y26 (= Y29) binding glyoxylate
- ATA 79:81 (≠ PTG 82:84) binding FMN
- S108 (= S111) binding FMN
- Q130 (= Q132) binding FMN
- Y132 (= Y134) binding glyoxylate
- T158 (= T160) binding FMN
- R167 (= R169) binding glyoxylate
- K236 (= K256) binding FMN
- S258 (= S278) binding FMN
- H260 (= H280) binding glyoxylate
- R263 (= R283) binding glyoxylate
- DGGVR 291:295 (≠ DSGIR 311:315) binding FMN
- GR 314:315 (= GR 334:335) binding FMN
2rduA Crystal structure of human glycolate oxidase in complex with glyoxylate (see paper)
36% identity, 90% coverage: 6:380/415 of query aligns to 1:358/360 of 2rduA
- active site: S106 (= S111), Y130 (= Y134), T156 (= T160), D158 (= D162), K234 (= K256), H258 (= H280)
- binding flavin mononucleotide: Y24 (= Y29), Y25 (≠ V30), A77 (≠ P82), T78 (= T83), A79 (≠ G84), S106 (= S111), Q128 (= Q132), Y130 (= Y134), T156 (= T160), K234 (= K256), H258 (= H280), G259 (= G281), R261 (= R283), D289 (= D311), G290 (≠ S312), G291 (= G313), R293 (= R315), G312 (= G334), R313 (= R335)
- binding glyoxylic acid: Y24 (= Y29), W108 (≠ V113), Y130 (= Y134), R165 (= R169), H258 (= H280), R261 (= R283)
1huvA Crystal structure of a soluble mutant of the membrane-associated (s)- mandelate dehydrogenase from pseudomonas putida at 2.15a resolution (see paper)
38% identity, 90% coverage: 11:382/415 of query aligns to 5:348/349 of 1huvA
- active site: S105 (= S111), Y128 (= Y134), T153 (= T160), D155 (= D162), K224 (= K256), H248 (= H280)
- binding flavin mononucleotide: Y23 (= Y29), P76 (= P82), T77 (= T83), G78 (= G84), S105 (= S111), Q126 (= Q132), Y128 (= Y134), T153 (= T160), K224 (= K256), H248 (= H280), G249 (= G281), R251 (= R283), D277 (= D311), S278 (= S312), G279 (= G313), R281 (= R315), G300 (= G334), R301 (= R335)
6gmbA Structure of human hydroxyacid oxidase 1 bound with fmn and glycolate
36% identity, 90% coverage: 6:380/415 of query aligns to 3:360/362 of 6gmbA
- active site: Y132 (= Y134), D160 (= D162), H260 (= H280)
- binding flavin mononucleotide: Y26 (= Y29), Y27 (≠ V30), A79 (≠ P82), T80 (= T83), A81 (≠ G84), S108 (= S111), Q130 (= Q132), Y132 (= Y134), K236 (= K256), H260 (= H280), G261 (= G281), R263 (= R283), D291 (= D311), G292 (≠ S312), G293 (= G313), R295 (= R315), G314 (= G334), R315 (= R335)
- binding glycolic acid: Y26 (= Y29), W110 (≠ V113), Y132 (= Y134), R167 (= R169), H260 (= H280), R263 (= R283)
2a85A Crystal structure of the g81a mutant of the active chimera of (s)- mandelate dehydrogenase in complex with its substrate 2- hydroxyoctanoate (see paper)
38% identity, 90% coverage: 11:382/415 of query aligns to 5:352/353 of 2a85A
- active site: S105 (= S111), Y128 (= Y134), T153 (= T160), D155 (= D162), K228 (= K256), H252 (= H280)
- binding flavin mononucleotide: Y23 (= Y29), P76 (= P82), T77 (= T83), A78 (≠ G84), S105 (= S111), Q126 (= Q132), Y128 (= Y134), T153 (= T160), K228 (= K256), H252 (= H280), G253 (= G281), R255 (= R283), D281 (= D311), S282 (= S312), G283 (= G313), R285 (= R315), G304 (= G334), R305 (= R335)
- binding (2s)-2-hydroxyoctanoic acid: Y128 (= Y134), N159 (≠ Q166), G160 (= G167), R162 (= R169), H252 (= H280), R255 (= R283)
2a7pA Crystal structure of the g81a mutant of the active chimera of (s)- mandelate dehydrogenase in complex with its substrate 3-indolelactate (see paper)
38% identity, 90% coverage: 11:382/415 of query aligns to 5:352/353 of 2a7pA
- active site: S105 (= S111), Y128 (= Y134), T153 (= T160), D155 (= D162), K228 (= K256), H252 (= H280)
- binding 3-(indol-3-yl) lactate: Y128 (= Y134), R162 (= R169), H252 (= H280)
- binding flavin mononucleotide: Y23 (= Y29), P76 (= P82), T77 (= T83), A78 (≠ G84), S105 (= S111), Q126 (= Q132), Y128 (= Y134), T153 (= T160), K228 (= K256), H252 (= H280), G253 (= G281), R255 (= R283), D281 (= D311), S282 (= S312), G283 (= G313), R285 (= R315), G304 (= G334), R305 (= R335)
5qigA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1407672867
36% identity, 90% coverage: 8:380/415 of query aligns to 2:357/359 of 5qigA
- active site: Y129 (= Y134), D157 (= D162), H257 (= H280)
- binding flavin mononucleotide: Y23 (= Y29), Y24 (≠ V30), A76 (≠ P82), T77 (= T83), A78 (≠ G84), S105 (= S111), Q127 (= Q132), Y129 (= Y134), T155 (= T160), K233 (= K256), H257 (= H280), G258 (= G281), R260 (= R283), D288 (= D311), G289 (≠ S312), G290 (= G313), R292 (= R315), G311 (= G334), R312 (= R335)
- binding 2-cyclopropyl-1~{H}-imidazole-4-carboxamide: L2 (≠ I8), R223 (≠ K246), G248 (= G271), F320 (≠ A343), K324 (≠ A347), D328 (≠ R351)
5qifA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z31792168
36% identity, 90% coverage: 8:380/415 of query aligns to 2:357/359 of 5qifA
- active site: Y129 (= Y134), D157 (= D162), H257 (= H280)
- binding flavin mononucleotide: Y23 (= Y29), Y24 (≠ V30), A76 (≠ P82), T77 (= T83), A78 (≠ G84), S105 (= S111), Q127 (= Q132), Y129 (= Y134), K233 (= K256), H257 (= H280), G258 (= G281), R260 (= R283), D288 (= D311), G289 (≠ S312), R292 (= R315), G311 (= G334), R312 (= R335)
- binding 2-cyclohexyl-~{N}-pyridin-3-yl-ethanamide: R223 (≠ K246), R224 (≠ K247), S227 (≠ G250)
2rdwA Crystal structure of human glycolate oxidase in complex with sulfate (see paper)
36% identity, 90% coverage: 8:380/415 of query aligns to 2:357/359 of 2rdwA