SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_082126415.1 NCBI__GCF_001013305.1:WP_082126415.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
56% identity, 90% coverage: 29:283/283 of query aligns to 4:257/257 of P0AAH0

query
sites
P0AAH0

V

V

E

E

S

T

A

A

K

P

V

S

K

K

M

I

R

Q

A

V

R

R

D

N

V

L

S

N

V

F

Y

Y

G

G

N

K

K

F

K

H

A

A

I

L

D

K

E

N

V

I

S

N

V

L

D

D

V

I

F

A

S

K

E

N

Y

Q

V

V

T

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

A

T

L

F

N

N

R

K

M

M

N

F

D

E

T

L

I

Y

P

P

S

E

A

Q

R

R

V

A

E

E

G

G

T

E

I

I

E

L

L

L

D

D

G

G

E

D

D

N

I

I

Y

L

S

T

S

N

G

S

M

Q

D

D

V

I

V

A

Q

L

L

L

R

R

A

A

R

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

K

P

P

N

T

P

P

F

F

P

P

K

M

S

S

I

I

Y

Y

D

D

N

N

V

I

A

A

Y

F

G

G

P

V

K

R

I

L

H

F

G

E

L

-

A

K

E

L

G

S

K

R

T

A

E

D

L

M

D

D

A

E

I

R

V

V

E

Q

K

W

S

A

L

L

T

T

R

K

A

A

G

A

L

L

W

W

E

N

E

E

V

T

K

K

D

D

R

K

L

L

Q

H

D

Q

S

S

G

G

T

Y

A

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

I

I

A

A

V

I

D

R

P

P

E

E

V

V

I

L

L

L

M

L

D

D

E

E

P

P

C

C

S

S

A

A

L

L

D

D

P

P

I

I

A

S

T

T

A

G

R

R

I

I

E

E

L

L

I

I

D

T

D

E

L

L

R

K

G

Q

R

D

Y

Y

A

T

I

V

V

V

I

I

V

V

T

T

H

H

S

N

M

M

Q

Q

A

A

R

R

V

C

S

S

Q

D

R

H

T

T

A

A

F

F

F

M

H

Y

L

L

G

G

H

E

L

L

V

I

E

E

Y

F

G

S

H

N

T

T

S

D

D

D

I

L

F

F

T

T

N

K

P

P

K

A

Q

K

R

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Q

E

D

D

Y

Y

I

I

T

T

G

G

R

R

Y

Y

G

G

G48 (= G73) mutation to I: Loss of phosphate transport.
K49 (= K74) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
35% identity, 86% coverage: 36:278/283 of query aligns to 3:236/241 of 4u00A

query
sites
4u00A

M

I

R

R

A

I

R

R

D

N

V

L

S

H

V

K

Y

W

Y
x
F

G

G

N

P

K

L

K

H

A
x
V

I

L

D

K

E

G

V

I

S

H

V

L

D

E

V

V

F

A

S

P

E

G

Y

E

V

K

T

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

A

T

L

I

N

N

R

R

M

L

N

E

D

D

T

F

I

-

P

-

S

-

A

-

R

-

V

Q

E

E

G

G

T

E

I

V

E

V

L

V

D

D

G

G

E

L

D

S

I

V

Y

K

S

D

G

-

M

R

D

A

V

L

V

R

Q

E

L

I

R

R

A

R

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

T

Y

L

G

A

P

P

-

M

K

R

I

V

H

R

G

R

L

W

A

P

E

R

G

E

K

K

T

A

E

E

L

K

D

K

A

A

I

L

V

-

E

-

K

E

S

L

L

L

T

E

R

R

A

V

G

G

L

I

W

L

E

-

V

-

K

-

D

D

R

Q

L

A

Q

R

D

K

S

Y

G

P

T

A

A

Q

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

R

E

I

V

E

L

E

D

L

V

I

M

D

R

D

D

L

L

-

A

R

Q

G

G

R

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

A

Q

D

R

R

T

V

A

V

F

F

F

M

H

D

L

G

G

G

H

Q

L

I

V

V

E

E

Y

E

G

G

H

R

T

P

S

E

D

E

I

I

F

F

T

T

N

R

P

P

K

K

Q

E

R

R

T

T

Q

R

D

S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y45), V17 (≠ A50), S37 (= S70), G38 (= G71), S39 (≠ C72), G40 (= G73), K41 (= K74), S42 (= S75), T43 (= T76)

3c4jA Abc protein artp in complex with atp-gamma-s
35% identity, 84% coverage: 45:282/283 of query aligns to 13:242/242 of 3c4jA

query
sites
3c4jA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

E

G

V

I

S

N

V

V

D

H

V

I

F

R

S

E

E

G

Y

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

P

-

S

-

A

-

R

-

V

-

E

E

G

G

T

E

I

I

E

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

K

K

I

V

H

R

G

K

L

W

A

P

E

R

G

E

K

K

T

A

E

E

L

A

D

K

A

A

I

M

V

-

E

-

K

E

S

L

L

L

T

D

R

K

A

V

G

G

L

L

W

-

E

-

V

-

K

K

D

D

R

K

L

A

Q

H

D

A

S

Y

G

P

T

D

A

S

L

L

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S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

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M

D

E

P

P

E

K

V

I

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M

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M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

R

E

I

V

E

L

E

S

L

V

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M

D

K

D

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

H

K

T

P

S

E

D

D

I

L

F

F

T

D

N

R

P

P

K

Q

Q

H

Q

E

R

R

T

T

Q

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y45), V18 (≠ A50), S38 (= S70), G39 (= G71), S40 (≠ C72), G41 (= G73), K42 (= K74), S43 (= S75), T44 (= T76)

3c41J Abc protein artp in complex with amp-pnp/mg2+
35% identity, 84% coverage: 45:282/283 of query aligns to 13:242/242 of 3c41J

query
sites
3c41J

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

E

G

V

I

S

N

V

V

D

H

V

I

F

R

S

E

E

G

Y

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

P

-

S

-

A

-

R

-

V

-

E

E

G

G

T

E

I

I

E

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

K

K

I

V

H

R

G

K

L

W

A

P

E

R

G

E

K

K

T

A

E

E

L

A

D

K

A

A

I

M

V

-

E

-

K

E

S

L

L

L

T

D

R

K

A

V

G

G

L

L

W

-

E

-

V

-

K

K

D

D

R

K

L

A

Q

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D
|
D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

R

E

I

V

E

L

E

S

L

V

I

M

D

K

D

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

H

K

T

P

S

E

D

D

I

L

F

F

T

D

N

R

P

P

K

Q

Q

H

Q

E

R

R

T

T

Q

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y45), V18 (≠ A50), S38 (= S70), G39 (= G71), G41 (= G73), K42 (= K74), S43 (= S75), T44 (= T76)
binding magnesium ion: S43 (= S75), D163 (= D204)

2olkA Abc protein artp in complex with adp-beta-s
35% identity, 84% coverage: 45:282/283 of query aligns to 13:242/242 of 2olkA

query
sites
2olkA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

E

G

V

I

S

N

V

V

D

H

V

I

F

R

S

E

E

G

Y

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

P

-

S

-

A

-

R

-

V

-

E

E

G

G

T

E

I

I

E

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

K

K

I

V

H

R

G

K

L

W

A

P

E

R

G

E

K

K

T

A

E

E

L

A

D

K

A

A

I

M

V

-

E

-

K

E

S

L

L

L

T

D

R

K

A

V

G

G

L

L

W

-

E

-

V

-

K

K

D

D

R

K

L

A

Q

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

R

E

I

V

E

L

E

S

L

V

I

M

D

K

D

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

H

K

T

P

S

E

D

D

I

L

F

F

T

D

N

R

P

P

K

Q

Q

H

Q

E

R

R

T

T

Q

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y45), V18 (≠ A50), S38 (= S70), G39 (= G71), S40 (≠ C72), G41 (= G73), K42 (= K74), S43 (= S75), T44 (= T76)

2oljA Abc protein artp in complex with adp/mg2+
35% identity, 84% coverage: 45:282/283 of query aligns to 13:242/242 of 2oljA

query
sites
2oljA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

E

G

V

I

S

N

V

V

D

H

V

I

F

R

S

E

E

G

Y

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

P

-

S

-

A

-

R

-

V

-

E

E

G

G

T

E

I

I

E

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

Y

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

K

K

I

V

H

R

G

K

L

W

A

P

E

R

G

E

K

K

T

A

E

E

L

A

D

K

A

A

I

M

V

-

E

-

K

E

S

L

L

L

T

D

R

K

A

V

G

G

L

L

W

-

E

-

V

-

K

K

D

D

R

K

L

A

Q

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

R

E

I

V

E

L

E

S

L

V

I

M

D

K

D

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

H

K

T

P

S

E

D

D

I

L

F

F

T

D

N

R

P

P

K

Q

Q

H

Q

E

R

R

T

T

Q

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding adenosine-5'-diphosphate: F13 (≠ Y45), V18 (≠ A50), S38 (= S70), G39 (= G71), S40 (≠ C72), G41 (= G73), K42 (= K74), S43 (= S75), T44 (= T76)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
35% identity, 85% coverage: 40:279/283 of query aligns to 6:237/240 of 4ymuJ

query
sites
4ymuJ

D

D

V

V

S

Y

V

K

Y

N

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

E

G

V

V

S

T

V

L

D

K

V

V

F

N

S

K

E

G

Y

E

V

V

T

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

A

C

L

I

N

N

R

L

M

L

N

E

D

E

T

-

I

-

P

P

S

T

A

-

R

-

V

-

E

K

G

G

T

E

I

V

E

F

L

I

D

D

G

G

E

V

D

K

I

I

Y

N

S

N

S

G

G

K

M

V

D

N

V

I

V

N

Q

K

L

V

R

R

A

Q

R

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

S

T

I

A

Y

I

D

E

N

N

V

I

A

T

Y

L

G

A

P

P

-

V

K

K

I

V

H

K

G

K

L

M

A

-

E

-

G

N

K

K

T

K

E

E

L

A

D

E

A

E

I

L

V

A

E

V

K

D

S

L

L

L

T

A

R

K

A

V

G

G

L

L

W

L

E

-

V

-

K

-

D

D

R

K

L

K

Q

D

D

Q

S

Y

G

P

T

I

A

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

Q

P

P

E

E

V

V

I

M

L

L

M

F

D

D

E
|
E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

K

R

E

I

V

E

L

E

N

L

V

I

M

D

K

D

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H
|
H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

I

F

F

F

M

H

D

L

D

G

G

H

V

L

I

V

V

E

E

Y

E

G

G

H

T

T

P

S

E

D

E

I

I

F

F

T

Y

N

R

P

A

K

K

Q

N

Q

E

R

R

T

T

Q

R

D

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y45), V16 (≠ A50), S36 (= S70), G37 (= G71), S38 (≠ C72), G39 (= G73), K40 (= K74), S41 (= S75), T42 (= T76), E162 (= E205), H194 (= H236)
binding magnesium ion: S41 (= S75), E162 (= E205)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 76% coverage: 63:278/283 of query aligns to 54:263/382 of 7ahhC

query
sites
7ahhC

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

A

L

L

L

N

N

R

R

M

L

N

I

D

E

T

-

I

-

P

P

S

T

A

S

R

-

V

-

E

-

G

G

T

K

I

I

E

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

K

E

I

V

H

Q

G

N

L

V

A

P

E

-

G

-

K

K

T

E

E

E

L

R

D

R

A

K

I

R

V

A

E

E

K

K

S

A

L

L

T

D

R

N

A

A

G

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

Q

P

D

K

S

Q

G

-

T

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D
|
D

E
|
E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

R

E

I

M

E

Q

E

D

L

E

I

L

D

L

D

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H

H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

H

T

T

G

S

E

D

E

I

I

F

L

T

T

N

N

P

P

K

A

Q

N

Q

D

R

Y

T

V

Q

K

D

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G71), G64 (= G73), K65 (= K74), D187 (= D204), E188 (= E205)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 76% coverage: 63:278/283 of query aligns to 54:263/382 of 7aheC

query
sites
7aheC

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

A

L

L

L

N

N

R

R

M

L

N

I

D

E

T

-

I

-

P

P

S

T

A

S

R

-

V

-

E

-

G

G

T

K

I

I

E

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

K

E

I

V

H

Q

G

N

L

V

A

P

E

-

G

-

K

K

T

E

E

E

L

R

D

R

A

K

I

R

V

A

E

E

K

K

S

A

L

L

T

D

R

N

A

A

G

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

Q

P

D

K

S

Q

G

-

T

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D

D

E

E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

R

E

I

M

E

Q

E

D

L

E

I

L

D

L

D

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H

H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

H

T

T

G

S

E

D

E

I

I

F

L

T

T

N

N

P

P

K

A

Q

N

Q

D

R

Y

T

V

Q

K

D

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 81% coverage: 49:278/283 of query aligns to 19:241/343 of P30750

query
sites
P30750

K

Q

A

A

I

L

D

N

E

N

V

V

S

S

V

L

D

H

V

V

F

P

S

A

E

G

Y

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

A

C

L

V

N

N

R

L

M

L

N

E

D

R

T

-

I

-

P

P

S

T

A

-

R

-

V

-

E

E

G

G

T

S

I

V

E

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

Y

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

K

E

I

L

H

D

G

N

L

T

A

P

E

-

G

-

K

K

T

D

E

E

L

V

D

K

A

R

I

R

V

V

E

T

K

E

S

L

L

L

T

S

R

L

A

V

G

G

L

L

W

G

E

-

V

-

K

-

D

D

R

K

L

H

Q

D

D

S

S

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

R

S

I

I

E

L

E

E

L

L

I

L

D

K

D

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

H

T

T

V

S

S

D

E

I

V

F

F

T

S

N

H

P

P

K

K

Q

T

Q

P

R

L

T

A

Q

Q

D

K

Y

F

I

I

40:46 (vs. 70:76, 86% identical) binding ATP
E166 (= E205) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

7ahdC Opua (e190q) occluded (see paper)
35% identity, 76% coverage: 63:278/283 of query aligns to 54:259/260 of 7ahdC

query
sites
7ahdC

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

A

L

L

L

N

N

R

R

M

L

N

I

D

E

T

-

I

-

P

P

S

T

A

S

R

-

V

-

E

-

G

G

T

K

I

I

E

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q
|
Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

Y

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

K

E

I

V

H

Q

G

N

L

V

A

P

E

-

G

-

K

K

T

E

E

E

L

R

D

R

A

K

I

R

V

A

E

E

K

K

S

A

L

L

T

D

R

N

A

A

G

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

Q

P

D
x
K

S
x
Q

G

-

T

-

A

-

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D

D

E
x
Q

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

R

E

I

M

E

Q

E

D

L

E

I

L

D

L

D

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H
|
H

S

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

H

T

T

G

S

E

D

E

I

I

F

L

T

T

N

N

P

P

K

-

Q

-

R

-

T

A

Q

N

D

D

Y

Y

I

V

binding adenosine-5'-triphosphate: S61 (= S70), G62 (= G71), G64 (= G73), K65 (= K74), S66 (= S75), T67 (= T76), Q111 (= Q123), K161 (≠ D175), Q162 (≠ S176), S164 (= S181), G166 (= G183), M167 (≠ Q184), Q188 (≠ E205), H221 (= H236)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
33% identity, 81% coverage: 49:278/283 of query aligns to 20:242/344 of 6cvlD

query
sites
6cvlD

K

Q

A

A

I

L

D

N

E

N

V

V

S

S

V

L

D

H

V

V

F

P

S

A

E

G

Y

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

A

C

L

V

N

N

R

L

M

L

N

E

D

R

T

-

I

-

P

P

S

T

A

-

R

-

V

-

E

E

G

G

T

S

I

V

E

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

Y

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

K

E

I

L

H

D

G

N

L

T

A

P

E

-

G

-

K

K

T

D

E

E

L

V

D

K

A

R

I

R

V

V

E

T

K

E

S

L

L

L

T

S

R

L

A

V

G

G

L

L

W

G

E

-

V

-

K

-

D

D

R

K

L

H

Q

D

D

S

S

Y

G

P

T

S

A
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

R

S

I

I

E

L

E

E

L

L

I

L

D

K

D

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H
|
H

S

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

H

T

T

V

S

S

D

E

I

V

F

F

T

S

N

H

P

P

K

K

Q

T

Q

P

R

L

T

A

Q

Q

D

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: S41 (= S70), G42 (= G71), A43 (≠ C72), G44 (= G73), K45 (= K74), S46 (= S75), T47 (= T76), N141 (≠ A179), S143 (= S181), Q146 (= Q184), H200 (= H236)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
33% identity, 81% coverage: 49:278/283 of query aligns to 20:242/344 of 3tuzC

query
sites
3tuzC

K

Q

A

A

I

L

D

N

E

N

V

V

S

S

V

L

D

H

V

V

F

P

S

A

E

G

Y

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

A

C

L

V

N

N

R

L

M

L

N

E

D

R

T

-

I

-

P

P

S

T

A

-

R

-

V

-

E

E

G

G

T

S

I

V

E

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

Y

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

K

E

I

L

H

D

G

N

L

T

A

P

E

-

G

-

K

K

T

D

E

E

L

V

D

K

A

R

I

R

V

V

E

T

K

E

S

L

L

L

T

S

R

L

A

V

G

G

L

L

W

G

E

-

V

-

K

-

D

D

R

K

L

H

Q

D

D

S

S

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

R

S

I

I

E

L

E

E

L

L

I

L

D

K

D

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

H

T

T

V

S

S

D

E

I

V

F

F

T

S

N

H

P

P

K

K

Q

T

Q

P

R

L

T

A

Q

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G71), G44 (= G73), K45 (= K74), S46 (= S75), T47 (= T76)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
33% identity, 81% coverage: 49:278/283 of query aligns to 20:242/344 of 3tuiC

query
sites
3tuiC

K

Q

A

A

I

L

D

N

E

N

V

V

S

S

V

L

D

H

V

V

F

P

S

A

E

G

Y

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

A

C

L

V

N

N

R

L

M

L

N

E

D

R

T

-

I

-

P

P

S

T

A

-

R

-

V

-

E

E

G

G

T

S

I

V

E

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

Y

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

K

E

I

L

H

D

G

N

L

T

A

P

E

-

G

-

K

K

T

D

E

E

L

V

D

K

A

R

I

R

V

V

E

T

K

E

S

L

L

L

T

S

R

L

A

V

G

G

L

L

W

G

E

-

V

-

K

-

D

D

R

K

L

H

Q

D

D

S

S

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

R

S

I

I

E

L

E

E

L

L

I

L

D

K

D

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

H

T

T

V

S

S

D

E

I

V

F

F

T

S

N

H

P

P

K

K

Q

T

Q

P

R

L

T

A

Q

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G71), A43 (≠ C72), G44 (= G73), K45 (= K74), S46 (= S75), T47 (= T76)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 87% coverage: 35:280/283 of query aligns to 6:255/258 of P02915

query
sites
P02915

K

K

M

L

R

H

A

V

R

I

D

D

V

L

S

H

V

K

Y

R

Y

Y

G

G

N

G

K

H

K

E

A

V

I

L

D

K

E

G

V

V

S

S

V

L

D

Q

V

A

F

R

S

A

E

G

Y

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

I

N

N

R

F

M

L

N

E

D

K

T

-

I

-

P

P

S

S

A

-

R

-

V

-

E

E

G

G

T

A

I

I

E

I

L

V

D

N

G

G

E

Q

D

N

I

I

-

N

-

L

-

V

-

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

Y

V

S

A

S

D

G

K

M

N

D

Q

V

L

V

R

Q

L

L

L

R

R

A

T

R

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

-

I

K

Q

I

V

H

L

G

G

L

L

A

S

E

K

G

H

K

D

T

A

E

R

L

E

D

R

A

A

I

L

V

-

E

-

K

K

S

Y

L

L

T

A

R

K

A

V

G

G

L

I

W

D

E

E

E

R

V

A

K

Q

D

G

R

K

L

Y

Q

P

D

-

S

-

G

-

T

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

D

V

V

I

L

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

L

T

V

A

G

R

E

I

V

E

L

E

R

L

I

I

M

D

Q

L

L

-

A

-

E

R

E

G

G

R

K

Y

-

A

T

I

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

Q

S

R

H

T

V

A

I

F

F

F

L

H

H

L

Q

G

G

H

K

L

I

V

E

E

E

Y

E

G

G

H

D

T

P

S

E

D

Q

I

V

F

F

T

G

N

N

P

P

K

Q

Q

S

Q

P

R

R

T

L

Q

Q

D

Q

Y

F

I

L

T

K

G

G

S41 (= S70) binding ATP
G42 (= G71) binding ATP
G44 (= G73) binding ATP
K45 (= K74) binding ATP
S46 (= S75) binding ATP
T47 (= T76) binding ATP

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
34% identity, 87% coverage: 35:280/283 of query aligns to 2:251/258 of 1b0uA

query
sites
1b0uA

K

K

M

L

R

H

A

V

R

I

D

D

V

L

S

H

V

K

Y

R

Y
|
Y

G

G

N

G

K
x
H

K

E

A

V

I

L

D

K

E

G

V

V

S

S

V

L

D

Q

V

A

F

R

S

A

E

G

Y

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

A

C

L

I

N

N

R

F

M

L

N

E

D

K

T

-

I

-

P

P

S

S

A

-

R

-

V

-

E

E

G

G

T

A

I

I

E

I

L

V

D

N

G

G

E

Q

D

N

I

I

-

N

-

L

-

V

-

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

Y

V

S

A

S

D

G

K

M

N

D

Q

V

L

V

R

Q

L

L

L

R

R

A

T

R

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

Y

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

-

I

K

Q

I

V

H

L

G

G

L

L

A

S

E

K

G

H

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D

T

A

E

R

L

E

D

R

A

A

I

L

V

-

E

-

K

K

S

Y

L

L

T

A

R

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A

V

G

G

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I

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D

E

E

E

R

V

A

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D

G

R

K

L

Y

Q

P

D

-

S

-

G

-

T

V

A

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L

L

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S

G

G

Q

Q

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R

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S

I

I

A

A

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R

A

A

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A

A

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D

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P

E

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V

V

I

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F

D

D

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E

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S

A

A

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L

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D

P

P

I

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A

L

T

V

A

G

R

E

I

V

E

L

E

R

L

I

I

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D

Q

L

L

-

A

-

E

R

E

G

G

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Y

-

A

T

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M

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V

I

V

V

V

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T

H

H

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E

M

M

Q

G

Q

F

A

A

A

R

R

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V

S

S

Q

S

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V

A

I

F

F

F

L

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H

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Q

G

G

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K

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E

E

E

Y

E

G

G

H

D

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P

S

E

D

Q

I

V

F

F

T

G

N

N

P

P

K

Q

Q

S

Q

P

R

R

T

L

Q

Q

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Q

Y

F

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G

G

binding adenosine-5'-triphosphate: Y12 (= Y45), H15 (≠ K48), S37 (= S70), G38 (= G71), G40 (= G73), K41 (= K74), S42 (= S75), T43 (= T76)

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
33% identity, 83% coverage: 30:265/283 of query aligns to 388:617/1286 of P21439

query
sites
P21439

E

D

S

S

A

I

K

K

V

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K

N

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R

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A

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N

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H

V

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Y

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Y
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Y

-

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-

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-
x
R

G

A

N

N

K

V

K

K

A

I

I

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K

E

G

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S

N

V

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S

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Q

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T

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A

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S

G
|
G

C
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C

G
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G

K
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K

S
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S

T
|
T

F

T

L

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R

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A

L

L

I

N

Q

R

R

M

L

N

Y

D

D

T

-

I

-

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P

S

D

A

-

R

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E
|
E

G

G

T

T

I

I

E

N

L

I

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D

G

G

E

Q

D
|
D

I

I

Y

R

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N

S

-

G

-

M

F

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N

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V

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N

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Y

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A

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Q

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P

N

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x
L

F

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I

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N

N

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Y

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-

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x
A

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x
E

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|
G

Q

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x
I

C

A

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A

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R

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A
|
A

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A

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I

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E

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A

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A

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A

A

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V

E

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E

A

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A

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D

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A

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E

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Y

R

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V

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A

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|
H

S
x
R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

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D

R

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T

I

A

A

F

G

F

F

H

E

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Y403 (= Y45) to H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
R406 (vs. gap) binding ATP; to Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
GCGKST 432:437 (= GCGKST 71:76) binding ATP
K435 (= K74) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (= E94) to G: in dbSNP:rs1189003716
D459 (= D103) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q123) binding ATP
P479 (= P125) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (≠ P127) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (≠ L165) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (≠ D175) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (= G182) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs1810849139
G536 (= G183) binding ATP; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ L188) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (= A193) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E205) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H236) to T: in GBD1; requires 2 nucleotide substitutions
R590 (≠ S237) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding ATP
1071:1077 binding ATP
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding ATP
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding ATP
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 80% coverage: 44:268/283 of query aligns to 14:230/280 of 5x40A

query
sites
5x40A

Y
x
F

Y

P

G

G

N

G

K
x
V

K

K

A
|
A

I

L

D

D

E

D

V

L

S

S

V

L

D

A

V

V

F

P

S

K

E

G

Y

E

V

S

T

L

A

A

F

I

I

L

G

G

P

P

S
x
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

L

A

H

L

L

N

N

R

-

M

-

N

G

D

T

T

L

I

R

P

P

S

Q

A

S

R

-

V

-

E

-

G

G

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I

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L

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G

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A

I

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Y

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A

R

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R

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Q

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D

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A

N

D

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D

-

Q

-

L

F

F

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A

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T

S

T

I

V

Y

F

D

E

N

D

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V

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N

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G

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A

A

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V

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A

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D

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T
x
H

A
x
M

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L

S
|
S

G

G

G
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G

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|
Q

Q

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Q

R

R

R

L

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C

A

I

I

A

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G

A

A

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A

A

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E

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V

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L

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D

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x
Q

P

P

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S

A

A

G

L

L

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D

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L

I

A

A

G

T

T

A

-

R

-

I

-

E

E

E

Q

L

L

I

L

D

T

D

L

L

L

R

R

G

G

R

L

Y

R

A

A

-

A

-

G

-

M

-

T

I

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V

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H
|
H

S

D

M

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E

Q

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A

A

R

A

V

L

S

A

Q

D

R

R

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A

A

F

L

F

F

H

R

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T

G

G

H

R

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V

L

E

A

Y

E

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G

H

A

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E

D

A

I

V

F

L

T

S

N

D

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y44), V18 (≠ K48), A20 (= A50), N40 (≠ S70), G41 (= G71), G43 (= G73), K44 (= K74), S45 (= S75), T46 (= T76), Q88 (= Q123), H139 (≠ T178), M140 (≠ A179), L141 (= L180), S142 (= S181), G144 (= G183), Q145 (= Q184), Q166 (≠ E205), H198 (= H236)
binding magnesium ion: S45 (= S75), Q88 (= Q123)

7niwA Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (see paper)
32% identity, 83% coverage: 30:265/283 of query aligns to 337:566/1140 of 7niwA

query
sites
7niwA

E

D

S

S

A

I

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K

V

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K

N

M

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E

A

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N

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D

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H

V

F

Y

S

Y

Y

-

P

-

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-

R

G

A

N

N

K

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K

A

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-

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S

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V

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R

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S

T

I

I

Y

A

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E

N

N

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Y

G

G

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K

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A

E

I

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-

E

K

K

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A

A

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A

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E

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G

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A

A

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G

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K

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C

A

I

I

A

A

R

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A

A

I

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A

V

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D

N

P

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K

V

I

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M

L

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A

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S

A

A

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I

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A

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A

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Q

E

A

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A

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L

D

D

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A

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A

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I

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A

H

H

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A

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A

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N

S

A

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I

A

A

F

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F

F

H

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V

E

E

Y

Q

G

G

H

S

T

H

S

S

D

E

I

L

Sites not aligning to the query:

binding cholesterol: 70, 152, 160, 266, 641, 645, 648, 839
binding 1,2-dilinoleoyl-sn-glycero-3-phosphocholine: 173, 183, 250, 254, 257, 261, 286, 294, 298, 617, 882
binding posaconazole: 250, 291, 294, 658, 661, 726, 841, 871, 874, 878, 882

6s7pA Nucleotide bound abcb4 (see paper)
32% identity, 83% coverage: 30:265/283 of query aligns to 327:556/1128 of 6s7pA

query
sites
6s7pA

E

D

S

S

A

I

K

K

V

G

K

N

M

L

R

E

A

F

R

N

D

D

V

V

S

H

V

F

Y

S

Y
|
Y

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P

-
x
S

-
x
R

G

A

N

N

K

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K

K

A

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D

K

E

G

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V

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T

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A

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S

S

G
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G

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C

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G

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S

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T

F

T

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A

L

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N

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-

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G

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T

I

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I

D

D

G

G

E

Q

D

D

I

I

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-

S

-

S

R

G

N

M

F

D

N

V

V

V

N

Q

Y

L

L

R

R

A

E

R

I

V

I

G

G

M

V

V

V

F

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Q
|
Q

K

E

P

P

N

V

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L

F

F

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K

T

S

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I

I

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A

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N

N

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I

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C

Y

Y

G

G

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-

K

-

I

-

H

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R

G

G

K

N

T

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D

D

A

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I

I

V

-

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K

K

K

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A

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L

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W

Y

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M

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K

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-

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D

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G

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x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

L

A

V

V

R

D

N

P

P

E

K

V

I

I

L

L

L

M

L

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

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D

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T

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A

A

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V

E

Q

E

A

L

A

I

L

D

D

D

K

L

A

R

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G

E

R

G

Y

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A

T

I

T

V

I

I

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I

T

A

H

H

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L

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-

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A

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V

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S

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A

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G

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V

L

I

V

V

E

E

Y

Q

G

G

H

S

T

H

S

S

D

E

I

L

binding adenosine-5'-triphosphate: Y342 (= Y45), S344 (vs. gap), R345 (vs. gap), G371 (= G71), G373 (= G73), K374 (= K74), S375 (= S75), Q416 (= Q123), Q471 (≠ A179), S473 (= S181), G475 (= G183), Q476 (= Q184)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 920, 922, 923, 948, 949, 951, 952, 953, 954, 994, 1033, 1053, 1054, 1056
binding cholesterol: 25, 32, 35, 65, 824, 834, 834, 838
binding magnesium ion: 953, 994

Query Sequence

>WP_082126415.1 NCBI__GCF_001013305.1:WP_082126415.1
MHSELNPPTEPGSPLADQTQVGAPSAENVESAKVKMRARDVSVYYGNKKAIDEVSVDVFS
EYVTAFIGPSGCGKSTFLRALNRMNDTIPSARVEGTIELDGEDIYSSGMDVVQLRARVGM
VFQKPNPFPKSIYDNVAYGPKIHGLAEGKTELDAIVEKSLTRAGLWEEVKDRLQDSGTAL
SGGQQQRLCIARAIAVDPEVILMDEPCSALDPIATARIEELIDDLRGRYAIVIVTHSMQQ
AARVSQRTAFFHLGHLVEYGHTSDIFTNPKQQRTQDYITGRYG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory