SitesBLAST

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
27% identity, 40% coverage: 19:185/420 of query aligns to 54:202/444 of Q8NLB7

query
sites
Q8NLB7

E
x
D

F

G

Y

Y

D
|
D

F

L

I

I

I

V

Y

Y

A

G

L

T

M

V

A

Q

V

S

Y

A

I

L

A

A

D

K

Q

E

F

W

F

N

P

L

S

S

H

S

D

A

S

T

F

L

T

G

S

T

M

I

L

G

T

S

T

T

F

-

A

-

T

-

F

-

S

-

V

-

G

A

Y

F

F

F

V

G

R

M

P

A

L

I

G

G

G

A

L

V

V

F

F

I

G

G

H

R

L

L

G

S

D

D

R
|
R

F

V

G

G

R

R

K

K

P

A

T

A

F

V

V

I

A

G

T

S

V

V

L

L

I

I

M

L

A

S

L

V

S

F

T

T

F

M

L

L

M

C

G

A

C

F

L

A

P

P

T

N

Y

-

S

-

Q

-

V

-

G

-

L

-

W

-

A

-

P

P

L

W

M

V

L

F

V

G

G

A

L

F

R

R

V

F

L

I

Q

A

G

G

F

L

S

G

I

L

G

G

E

L

I

V

P

P

G

S

A

V

M

N

T

A

Y

M

L

T

S

S

E

D

T

L

V

V

D

P

R

R

R

K

R

-

G

-

L

-

V

T

M

M

S

S

L

A

L

W

F

A

M

T

V

V

L

M

A

M

N

S

G

G

V

V

V

P

F

I

G

G

S

G

L

S

V

I

H

A

A

A

F

-

M

V

L

L

W

A

W

L

L

V

P

V

V

V

D

P

E

S

M

S

K

E

A

E

W

W

G

G

W

W

R

R

I

F

P

M

F

F

D54 (≠ E19) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D22) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R74) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

8fvzA Pipt y150a
29% identity, 37% coverage: 8:163/420 of query aligns to 5:158/433 of 8fvzA

query
sites
8fvzA

L

L

I

V

L

L

V

L

T

A

S

G

L

V

G

G

G

F

F

F

L

L

E

D

F

A

Y

Y

D

D

F

L

I

F

I

I

Y

I

A

N

L

Q

M

V

A

A

V

P

Y

M

I

L

A

A

D

Q

Q

V

F

Y

F

F

P

P

S

K

H

T

D

G

S

L

F

P

T

A

S

Q

M

R

L

Q

T

D

T

L

F

M

-

K

A

A

T

A

F

A

S

N

V

I

G

G

Y

C

F

V

V

V

R

-

P

-

L

-

G

G

G

Q

L

V

V

M

F

F

G

G

H

V

L

L

G

G

D

D

R

S

F

F

G

G

R

R

K

K

P

F

T

V

F

Y

V

G

A

K

T

E

V

L

L

I

I

L

M

I

A

I

L

V

S

A

T

T

F

I

L

F

M

Q

G

M

C

S

L

A

P

P

T

S

Y

H

-

W

-

D

-

G

S

N

Q

R

V

V

G

L

L

T

W

W

A

I

P

T

L

I

M

-

L

-

V

-

G

-

L

C

R

R

V

V

L

F

Q

L

G

G

F

I

S

G

I

I

G

G

E

D

I

A

P

P

G

M

A

S

M

A

T

T

Y

V

L

V

S

S

E

D

T

R

V

A

D

N

-

I

R

H

R

R

G

G

L

T

V

L

M

L

S

C

L

F

L

I

F
|
F

M

A

V

N

L

Q

A

G

N

W

G

G

V

S

V

F

F

V

G

G

S

S

L

L

V

V

binding phosphate ion: F145 (= F150)

Sites not aligning to the query:

binding phosphate ion: 235, 239, 243, 346

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
37% identity, 23% coverage: 69:165/420 of query aligns to 78:167/452 of Q5EXK5

query
sites
Q5EXK5

G

G

H

P

L

L

G

S

D
|
D

R

R

F

F

G

G

R

R

K

K

P

R

T

V

F

I

V

E

A

F

T

C

V

V

L

A

I

L

M

F

A

G

L

L

S

F

T

S

F

L

L

L

M

S

G

A

C

F

L

A

P

P

T

D

Y

L

S

Q

Q

-

V

-

G

-

L

-

W

-

A

-

P

-

L

-

M

M

L

L

V

V

G

F

L

L

R

R

V

F

L

L

Q

T

G

G

F

L

S

G

I

L

G

G

E

A

I

M

P

P

G

N

A

T

M

I

T

T

Y

M

L

T

S

S

E

E

T

Y

V

L

D

P

-

A

R

R

G

G

L

A

V

L

M

V

S

T

L

L

L

M

F

F

M

C

V

G

L

F

A

T

N

L

G

G

V

S

V

A

F

F

G

G

S

G

L

V

V

V

H

S

A

A

D82 (= D73) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

O42885 Putative inorganic phosphate transporter C8E4.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 25% coverage: 63:168/420 of query aligns to 108:214/572 of O42885

query
sites
O42885

L

F

G

G

G

Q

L

L

V

L

F

F

G

G

H

F

L

M

G

G

D

D

R

F

F

F

G

G

R

R

K

K

P

F

T

V

F

Y

V

G

A

K

T

E

V

M

L

V

I

I

M

V

A

I

L

I

S

A

T

T

F

V

L

L

M

V

G

I

C

A

L

M

P

P

T

K

Y

S

S

I

Q

H

V

S

G

P

L

L

W

S

A

K

P

M

L

M

M

W

L

V

V

F

G

C

L

W

R

R

V

W

L

L

Q

L

G

G

F

V

S

G

I

I

G

G

E

D

I

Y

P

P

G

M

A

S

M

A

T

A

Y

I

L

T

S

S

E

E

T

R

V

S

D

K

-

I

R

K

R

R

G

G

L

T

V

L

M

I

S

S

L

L

L

I

F

F

M

A

V

F

L

Q

A

G

N

F

G

G

V

T

V

L

F

A

G

G

S

A

L

I

V

V

H

T

A

I

F

I

M

L

L

L

Sites not aligning to the query:

12 modified: Phosphoserine
14 modified: Phosphoserine
292 modified: Phosphoserine
296 modified: Phosphoserine

Q09852 Putative inorganic phosphate transporter C23D3.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 25% coverage: 64:168/420 of query aligns to 104:209/559 of Q09852

query
sites
Q09852

G

G

G

Q

L

I

V

L

F

F

G

G

H

F

L

M

G

G

D

D

R

F

F

F

G

G

R

R

K

K

P

F

T

V

F

Y

V

G

A

K

T

E

V

M

L

I

I

V

M

V

A

I

L

I

S

A

T

T

F

V

L

L

M

V

G

I

C

A

L

L

P

P

T

K

Y

S

S

I

Q

P

V

T

G

P

L

L

W

G

A

K

P

M

L

M

M

W

L

I

V

F

G

A

L

W

R

R

V

W

L

L

Q

L

G

G

F

L

S

G

I

I

G

G

E

D

I

Y

P

P

G

M

A

S

M

A

T

T

Y

I

L

T

S

S

E

E

-

R

T

S

V

L

D

L

R

S

R

R

G

G

L

T

V

L

M

L

S

S

L

I

L

V

F

F

M

S

V

F

L

Q

A

G

N

F

G

G

V

T

V

L

F

A

G

G

S

A

L

I

V

V

H

T

A

I

F

I

M

L

L

L

Sites not aligning to the query:

297 modified: Phosphoserine
299 modified: Phosphoserine

P10870 Low glucose sensor SNF3; High-affinity glucose receptor SNF3; High-affinity transporter-like sensor SNF3; Sucrose nonfermenting protein 3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
29% identity, 42% coverage: 13:187/420 of query aligns to 105:269/884 of P10870

query
sites
P10870

S

A

L

V

G

G

F

F

L

L

E

F

F
x
G

Y

Y

D

D

F

T

-

G

I

L

I

I

Y

N

A

S

L

I

M

T

A

S

V

M

-

N

Y

Y

I

V

A

K

D

S

Q

H

F

V

F

A

P

P

S

N

H

H

D

D

S

S

F

F

T

T

-

A

-

Q

-

M

S

S

M

I

L

L

T

V

T

S

F

F

A

-

T

-

F

L

S

S

V

L

G
|
G

Y

T

F

F

V

-

R

-

P

-

L

F

G

G

G

A

L

L

V

T

F

A

G

P

H

F

L

I

G

S

D

D

R

S

F

Y

G

G

R

R

K

K

P

P

T

T

F

I

V

I

-

F

A

S

T

T

V

I

L

F

I

I

M

F

A

S

L

I

S

G

T

N

F

S

L

L

M

-

G

-

C

-

L

-

P

-

T

-

Y

-

S

-

Q

Q

V

V

G

G

L

A

W

G

A

G

P

I

L

T

M

L

L

L

V

I

G

V

L

G

R

R

V

V

L

I

Q

S

G

G

F

I

S

G

I

I

G

G

G

A

E

I

I

S

P

A

G

V

A

V

M

P

T

L

Y

Y

L

Q

S

A

E

E

T

A

V

T

D

H

R

K

R

S

-

L

R
|
R

G

G

L

A

V

I

M

I

S

S

L

T

L

Y

F

Q

M

W

V

A

L

I

A

T

N

W

G

G

V

L

F

V

G

S

S

S

L

A

V

V

-

S

-

Q

-

G

-

T

H

H

A

A

F

-

M

-

L

-

W

-

L

-

P

-

V

-

D

-

E

R

M

N

K

D

A

A

W

S

G

S

W

Y

R

R

I

I

P

P

F

I

W

G

L

L

G112 (≠ F20) mutation to D: In SNF3-142.
G153 (= G57) mutation to R: In SNF3-72.
R229 (= R142) mutation to K: In SNF3-1; constitutively signaling glucose receptor.

Sites not aligning to the query:

374 I→V: Decreases responsiveness to mannose and fructose.
402 V→I: In SNF3-39.
462 F→Y: Decreases responsiveness to fructose.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
31% identity, 30% coverage: 65:189/420 of query aligns to 76:190/448 of Q51955

query
sites
Q51955

G

G

L

M

V

V

F

F

G

G

H

A

L

L

G

G

-

S

-
x
G

-

P

-

L

-

A

D
|
D

R

R

F

F

G
|
G

R

R

K

K

P

G

T

V

F

L

V

V

A

G

T

A

V

V

L

L

I

V

M

F

A

G

L

G

S

F

T

S

F

L

L

A

M

S

G

A

C

Y

L

A

P

T

T

N

Y

V

S

D

Q

Q

V

-

G

-

L

-

W

-

A

-

P

-

L

-

M

-

L

L

V

L

G

V

L

L

R
|
R

V

F

L

L

Q

T

G

G

F

L

S

G

I

L

G

G

G

A

E

G

I

M

P

P

G

N

A

A

M

T

T

T

Y

L

L

L

S

S

E
|
E

-

Y

T

T

V

P

D

E

R

R

R

L

R

K

G

S

L

L

V

L

M

V

S

T

L

S

L

M

F

F

M

C

V

G

L

F

A

N

N

L

G

G

V

M

V

A

F

G

G

G

S

G

L

F

V

I

H

S

A

A

F

K

M

M

L

I

W

-

L

-

P

-

V

-

D

-

E

-

M

-

K

P

A

A

W

Y

G

G

W

W

R
x
H

I

S

P

L

F

L

W

V

L

I

G

G

G85 (vs. gap) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D73) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G76) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R116) mutation to A: Abolishes 4-HBA transport.
E144 (= E136) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ R182) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

41 mutation D->A,N: Abolishes 4-HBA transport.; D→E: Decrease in 4-HBA transport.
44 mutation D->A,N: Abolishes 4-HBA transport.; D→E: Decrease in 4-HBA transport.
323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

8et8A Cryo-em structure of the organic cation transporter 1 in complex with verapamil (see paper)
33% identity, 25% coverage: 55:160/420 of query aligns to 155:256/532 of 8et8A

query
sites
8et8A

S

N

V

V

G

G

Y

F

F

F

V

-

R

-

P

-

L

L

G

G

G

S

L

L

V

G

F

V

G

G

H

Y

L

I

G

A

D

D

R

R

F

F

G

G

R

R

K

K

P

L

T

C

F

L

V

L

A

A

T

T

V

T

L

L

I

I

M

S

A

A

L

V

S

S

T

G

F

V

L

L

M

M

G

A

C

V

L

A

P

P

T

D

Y

Y

S

T

Q

S

V

M

G

L

L

L

W

F

A

-

P

-

L

-

M

-

L

-

V

-

G

-

L

-

R

R

V

L

L

L

Q

Q

G

G

F

L

S

V

I

S

G
x
K

G

G

E

S

I
x
W

P

M

G

S

A

G

M

Y

T

T

Y

L

L

I

S

T

E

E

T

F

V

V

D

G

R

S

R

G

R

Y

G

R

L

R

V

T

M

V

S

A

L

I

L

L

F

Y

-
x
Q

-

M

-

A

-

F

-

T

-

V

-

G

M

L

V

V

L

L

A

L

N

S

G

G

V

V

V

A

F

Y

G

A

binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: K213 (≠ G124), W216 (≠ I127), Q240 (vs. gap)

Sites not aligning to the query:

binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: 31, 35, 353, 360, 378, 381, 385, 449, 469

8et7A Cryo-em structure of the organic cation transporter 1 in complex with diphenhydramine (see paper)
33% identity, 25% coverage: 55:160/420 of query aligns to 155:256/532 of 8et7A

query
sites
8et7A

S

N

V

V

G

G

Y

F

F

F

V

-

R

-

P

-

L

L

G

G

G

S

L

L

V

G

F

V

G

G

H

Y

L

I

G

A

D

D

R

R

F

F

G

G

R

R

K

K

P

L

T

C

F

L

V

L

A

A

T

T

V

T

L

L

I

I

M

S

A

A

L

V

S

S

T

G

F

V

L

L

M

M

G

A

C

V

L

A

P

P

T

D

Y

Y

S

T

Q

S

V

M

G

L

L

L

W

F

A

-

P

-

L

-

M

-

L

-

V

-

G

-

L

-

R

R

V

L

L

L

Q

Q

G

G

F

L

S

V

I

S

G

K

G

G

E

S

I

W

P

M

G

S

A

G

M

Y

T

T

Y

L

L

I

S

T

E

E

T

F

V

V

D

G

R

S

R

G

R

Y

G

R

L

R

V

T

M

V

S

A

L

I

L

L

F

Y

-

Q

-

M

-

A

-

F

-

T

-

V

-

G

M

L

V

V

L

L

A

L

N

S

G

G

V

V

V

A

F

Y

G

A

Sites not aligning to the query:

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: 35, 385, 445

Query Sequence

>WP_082212236.1 NCBI__GCF_001583435.1:WP_082212236.1
MSREHKGLILVTSLGGFLEFYDFIIYALMAVYIADQFFPSHDSFTSMLTTFATFSVGYFV
RPLGGLVFGHLGDRFGRKPTFVATVLIMALSTFLMGCLPTYSQVGLWAPLMLVGLRVLQG
FSIGGEIPGAMTYLSETVDRRRGLVMSLLFMVLANGVVFGSLVHAFMLWWLPVDEMKAWG
WRIPFWLGGTMGICSYVVRKQFQESDLFLQLAKRKAQSAVPLFHLLQSHRRQLLVGIFLI
LPVATSMTLLFLFTPGYLTKMLGYSAGDVALAGSIGIFLSSVVFIAFGLLADTVSLRSLL
CVASGIIVLFSVPVFYWYGAGVDVYSVMLVSALIQGSITGVAPLALSELFPVNVRYSGIA
FCYNVSFALFGGLTPVIAMTLIGWTNNLQSPAWYLMVSGLFGLLATCMMSVPRRPSAAQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory