SitesBLAST
Comparing WP_083441911.1 NCBI__GCF_000969705.1:WP_083441911.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
46% identity, 98% coverage: 3:242/245 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G16), N111 (≠ H111), S139 (= S140), Q149 (= Q150), Y152 (= Y153), K156 (= K157)
- binding acetoacetyl-coenzyme a: D93 (= D93), K98 (≠ R98), S139 (= S140), N146 (= N147), V147 (≠ M148), Q149 (= Q150), Y152 (= Y153), F184 (≠ L185), M189 (= M190), K200 (≠ E201)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ R15), G18 (= G16), I19 (≠ L17), D38 (= D36), F39 (≠ L37), V59 (≠ C59), D60 (= D60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (= I90), T137 (≠ L138), S139 (= S140), Y152 (= Y153), K156 (= K157), P182 (= P183), F184 (≠ L185), T185 (≠ I186), T187 (= T188), M189 (= M190)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
46% identity, 99% coverage: 1:242/245 of query aligns to 4:244/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (= R15), G19 (= G16), I20 (≠ L17), D39 (= D36), R40 (≠ L37), C63 (= C59), I65 (≠ V61), N91 (= N87), G93 (= G89), I94 (= I90), V114 (= V110), Y155 (= Y153), K159 (= K157), I188 (= I186), T190 (= T188), T193 (= T191)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
46% identity, 98% coverage: 3:242/245 of query aligns to 3:236/239 of 4nbtA
- active site: G16 (= G16), S132 (= S140), Y145 (= Y153), K149 (= K157)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ R15), G16 (= G16), L17 (= L17), D36 (= D36), L37 (= L37), L52 (≠ C59), N53 (≠ D60), V54 (= V61), N80 (= N87), A81 (= A88), G82 (= G89), I130 (≠ L138), S132 (= S140), Y145 (= Y153), K149 (= K157), P177 (= P183), G178 (= G184), I180 (= I186), T182 (= T188)
7krmC Putative fabg bound to nadh from acinetobacter baumannii
45% identity, 99% coverage: 1:242/245 of query aligns to 1:240/244 of 7krmC
- active site: G18 (= G16), S140 (= S140), Y155 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S15 (vs. gap), G18 (= G16), I19 (≠ L17), D38 (= D36), L39 (= L37), A60 (≠ C59), N61 (≠ D60), V62 (= V61), N88 (= N87), V111 (= V110), S140 (= S140), Y155 (= Y153), K159 (= K157), I188 (= I186), T190 (= T188)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
43% identity, 100% coverage: 1:245/245 of query aligns to 1:247/247 of 4jroC
- active site: G16 (= G16), S142 (= S140), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), G16 (= G16), I17 (≠ L17), N35 (≠ D36), Y36 (≠ L37), N37 (≠ D38), G38 (= G39), S39 (vs. gap), N63 (≠ D60), V64 (= V61), N90 (= N87), A91 (= A88), I93 (= I90), I113 (≠ V110), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), I188 (= I186), T190 (= T188)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
42% identity, 100% coverage: 1:245/245 of query aligns to 1:245/248 of Q9KJF1
- M1 (= M1) modified: Initiator methionine, Removed
- S15 (≠ R15) binding NAD(+)
- D36 (= D36) binding NAD(+)
- D62 (= D60) binding NAD(+)
- I63 (≠ V61) binding NAD(+)
- N89 (= N87) binding NAD(+)
- Y153 (= Y153) binding NAD(+)
- K157 (= K157) binding NAD(+)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
42% identity, 99% coverage: 3:245/245 of query aligns to 2:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), M16 (≠ L17), D35 (= D36), I36 (≠ L37), I62 (≠ V61), N88 (= N87), G90 (= G89), I138 (≠ L138), S140 (= S140), Y152 (= Y153), K156 (= K157), I185 (= I186)
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
45% identity, 99% coverage: 3:245/245 of query aligns to 3:239/240 of 4dmmB
- active site: G16 (= G16), S142 (= S140), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), G16 (= G16), I17 (≠ L17), A37 (vs. gap), S38 (≠ A35), S39 (≠ D36), A62 (≠ C59), D63 (= D60), V64 (= V61), N90 (= N87), A91 (= A88), L113 (≠ V110), I140 (≠ L138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), I188 (= I186), T190 (= T188), M192 (= M190)
4cqmA Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
41% identity, 99% coverage: 3:245/245 of query aligns to 4:248/248 of 4cqmA
- active site: G17 (= G16), S143 (= S140), Y156 (= Y153), K160 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), S16 (≠ R15), G17 (= G16), I18 (≠ L17), D37 (= D36), L38 (= L37), A61 (≠ C59), V63 (= V61), C90 (≠ N87), A91 (= A88), G92 (= G89), I93 (= I90), V113 (= V110), I141 (≠ L138), S143 (= S140), Y156 (= Y153), K160 (= K157), P186 (= P183), G187 (= G184), I189 (= I186), T191 (= T188), P192 (≠ A189), M193 (= M190), T194 (= T191)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
43% identity, 98% coverage: 3:242/245 of query aligns to 4:248/252 of 1vl8B
- active site: G17 (= G16), S143 (= S140), I154 (≠ T151), Y157 (= Y153), K161 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), R16 (= R15), G17 (= G16), L18 (= L17), S37 (≠ D36), R38 (≠ L37), C63 (= C59), D64 (= D60), V65 (= V61), A91 (≠ N87), A92 (= A88), G93 (= G89), I94 (= I90), V114 (= V110), I141 (≠ L138), S143 (= S140), Y157 (= Y153), K161 (= K157), P187 (= P183), G188 (= G184), Y190 (≠ I186), T192 (= T188), M194 (= M190), T195 (= T191)
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
41% identity, 99% coverage: 3:245/245 of query aligns to 6:251/251 of 4cqlI
- active site: G19 (= G16), S146 (= S140), Y159 (= Y153), K163 (= K157)
- binding nicotinamide-adenine-dinucleotide: S18 (≠ R15), G19 (= G16), I20 (≠ L17), D39 (= D36), L40 (= L37), A64 (≠ C59), D65 (= D60), V66 (= V61), C93 (≠ N87), A94 (= A88), G95 (= G89), I96 (= I90), V116 (= V110), I144 (≠ L138), S146 (= S140), Y159 (= Y153), K163 (= K157), P189 (= P183), G190 (= G184), I192 (= I186), T194 (= T188), M196 (= M190)
Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
40% identity, 99% coverage: 3:245/245 of query aligns to 9:261/261 of Q92506
- 15:23 (vs. 9:17, 44% identical) binding NAD(+)
- D42 (= D36) mutation to A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- DL 42:43 (= DL 36:37) binding NAD(+)
- ADV 74:76 (≠ CDV 59:61) binding NAD(+)
- R148 (≠ G132) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- V158 (≠ S142) to L: in a breast cancer sample; somatic mutation
- Y169 (= Y153) mutation to A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- YAASK 169:173 (≠ YSAAK 153:157) binding NAD(+)
- K173 (= K157) mutation to A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- R189 (= R173) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- IAT 202:204 (≠ IRT 186:188) binding NAD(+)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
40% identity, 98% coverage: 6:245/245 of query aligns to 5:246/246 of 3osuA
P73826 Acetoacetyl-CoA reductase; EC 1.1.1.36 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
43% identity, 98% coverage: 3:241/245 of query aligns to 6:236/240 of P73826
- S134 (= S140) mutation to A: 12% enzymatic activity.
- Y147 (= Y153) mutation to A: No enzymatic activity.
- K151 (= K157) mutation to A: 5% enzymatic activity.
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
39% identity, 100% coverage: 1:245/245 of query aligns to 4:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (≠ L17), T40 (≠ V41), N62 (≠ D60), V63 (= V61), N89 (= N87), A90 (= A88), I92 (= I90), V139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), I187 (= I186), T189 (= T188), M191 (= M190)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
40% identity, 99% coverage: 3:245/245 of query aligns to 4:246/247 of P73574
- A14 (≠ G13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ M148) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K157) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ L185) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ P195) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
4nbwA Crystal structure of fabg from plesiocystis pacifica (see paper)
43% identity, 97% coverage: 5:242/245 of query aligns to 1:248/253 of 4nbwA
- active site: G12 (= G16), S146 (= S140), Y159 (= Y153), K163 (= K157)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), N11 (≠ R15), G12 (= G16), I13 (≠ L17), D32 (= D36), L33 (= L37), V57 (≠ C59), D58 (= D60), V59 (= V61), N85 (= N87), A86 (= A88), G87 (= G89), S146 (= S140), Y159 (= Y153), K163 (= K157), I192 (= I186), T194 (= T188)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
40% identity, 98% coverage: 6:245/245 of query aligns to 2:239/239 of 3sj7A
- active site: G12 (= G16), S138 (= S140), Q148 (= Q150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (= R15), I13 (≠ L17), N31 (≠ D36), Y32 (≠ L37), A33 (≠ D38), G34 (= G39), S35 (≠ D40), A58 (≠ C59), N59 (≠ D60), V60 (= V61), N86 (= N87), A87 (= A88), T109 (≠ V110), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
40% identity, 96% coverage: 7:242/245 of query aligns to 3:241/244 of 1edoA
- active site: G12 (= G16), S138 (= S140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (= R15), I13 (≠ L17), N31 (≠ D38), Y32 (≠ G39), A33 (≠ D40), R34 (≠ V41), S35 (≠ A42), D59 (= D60), V60 (= V61), N86 (= N87), A87 (= A88), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (= I186), S186 (≠ T188), M188 (= M190)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
39% identity, 100% coverage: 1:245/245 of query aligns to 4:242/243 of 4i08A
- active site: G19 (= G16), N113 (≠ H111), S141 (= S140), Q151 (= Q150), Y154 (= Y153), K158 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (≠ L17), T40 (≠ V41), N62 (≠ D60), V63 (= V61), N89 (= N87), A90 (= A88), G140 (≠ S139), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), T189 (= T188)
Query Sequence
>WP_083441911.1 NCBI__GCF_000969705.1:WP_083441911.1
MLLQERVAIVTGGARGLGLAIAERYVEQGARVVLADLDGDVADGAATALSDERAHGAACD
VTSEEDVQRLFDETIERHGRVDVLVNNAGITRDAMAHRMSLADFRQVVDVHLQGTWLGTR
AALTHMRGREGGGAIVNLSSLSGKIGNMGQTNYSAAKAGIVGLTKAAAKEGARFGIRANA
IQPGLIRTAMTEAMPEDVLAERIGGIPLGRMGEPAEIADVALFLASDLSSYVTGAVIEVT
GGRGM
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory