SitesBLAST
Comparing WP_083453266.1 NCBI__GCF_001293685.1:WP_083453266.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1jroA Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus (see paper)
35% identity, 96% coverage: 1:455/474 of query aligns to 1:433/450 of 1jroA
- binding flavin-adenine dinucleotide: G42 (= G42), D43 (≠ E43), L189 (≠ V206), A191 (= A208), G192 (= G209), G193 (= G210), T194 (= T211), D195 (= D212), V196 (≠ L213), F258 (≠ L277), A259 (≠ G278), T268 (= T287), G271 (= G290), N272 (= N291), A274 (= A293), G280 (= G299), D281 (= D300), R318 (≠ L337), V324 (≠ I343)
- binding fe2/s2 (inorganic) cluster: E37 (= E37), G38 (= G38), C39 (= C39), N40 (≠ G40), G42 (= G42), C44 (= C44), G45 (= G45), C47 (= C47), C63 (= C59), C103 (= C98), C106 (= C101), C134 (= C133), C136 (= C135)
2w54A Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus in complex with bound inhibitor pterin-6-aldehyde (see paper)
34% identity, 96% coverage: 1:455/474 of query aligns to 1:433/450 of 2w54A
- binding flavin-adenine dinucleotide: G42 (= G42), L189 (≠ V206), A191 (= A208), G192 (= G209), G193 (= G210), T194 (= T211), D195 (= D212), V196 (≠ L213), W199 (= W216), L213 (= L230), F258 (≠ L277), A259 (≠ G278), A267 (≠ G286), T268 (= T287), G271 (= G290), N272 (= N291), A274 (= A293), N275 (≠ T294), G280 (= G299), D281 (= D300), R318 (≠ L337), V324 (≠ I343), Q347 (= Q366)
- binding fe2/s2 (inorganic) cluster: G38 (= G38), C39 (= C39), N40 (≠ G40), G42 (= G42), C44 (= C44), G45 (= G45), C47 (= C47), C63 (= C59), C103 (= C98), G104 (= G99), C106 (= C101), C134 (= C133), R135 (= R134), C136 (= C135)
- binding {[(5aR,8R,9aR)-2-amino-4-oxo-6,7-di(sulfanyl-kappaS)-3,5,5a,8,9a,10-hexahydro-4H-pyrano[3,2-g]pteridin-8-yl]methyl dihydrogenato(2-) phosphate}(hydroxy)oxo(thioxo)molybdenum: Q102 (= Q97), C136 (= C135)
4uhxA Human aldehyde oxidase in complex with phthalazine and thioridazine (see paper)
29% identity, 84% coverage: 58:456/474 of query aligns to 70:492/1290 of 4uhxA
- binding flavin-adenine dinucleotide: P229 (≠ R205), V230 (= V206), I231 (≠ F207), M232 (≠ A208), G233 (= G209), N234 (≠ G210), T235 (= T211), S236 (≠ D212), V237 (≠ L213), L310 (= L277), A311 (≠ G278), A319 (≠ G286), S320 (≠ T287), G323 (= G290), H324 (≠ N291), I326 (≠ A293), H329 (≠ S296), S332 (≠ G299), D333 (= D300), L377 (≠ I343), L404 (≠ C370)
- binding fe2/s2 (inorganic) cluster: C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
Sites not aligning to the query:
- active site: 732, 767, 843, 847, 875, 1223, 1224
- binding flavin-adenine dinucleotide: 43, 44
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69
- binding 10-{2-[(2S)-1-methylpiperidin-2-yl]ethyl}-2-(methylsulfanyl)-10H-phenothiazine: 540, 542, 543, 1014, 1015, 1018, 1019, 1020, 1079
- binding 10-{2-[(2R)-1-methylpiperidin-2-yl]ethyl}-2-(methylsulfanyl)-10H-phenothiazine: 540, 542, 543, 1014, 1015, 1018, 1019, 1020
3zyvB Crystal structure of the mouse liver aldehyde oxidase 3 (maox3) (see paper)
30% identity, 96% coverage: 5:459/474 of query aligns to 6:483/1262 of 3zyvB
- binding flavin-adenine dinucleotide: L227 (≠ V206), I229 (≠ A208), G230 (= G209), N231 (≠ G210), T232 (= T211), Y233 (≠ D212), L234 (= L213), T267 (vs. gap), L303 (= L277), A304 (≠ G278), A312 (≠ G286), S313 (≠ T287), G316 (= G290), H317 (≠ N291), I319 (≠ A293), S325 (≠ G299), D326 (= D300), L366 (≠ I343), A392 (= A369), F393 (≠ C370)
- binding fe2/s2 (inorganic) cluster: Y39 (≠ E37), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), C49 (= C47), T69 (vs. gap), C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
Sites not aligning to the query:
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
53% identity, 31% coverage: 1:147/474 of query aligns to 4:150/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ E37), C42 (= C39), E43 (≠ G40), G45 (= G42), C47 (= C44), G48 (= G45), C50 (= C47), R60 (≠ L57), C62 (= C59), C101 (= C98), G102 (= G99), C104 (= C101), C136 (= C133), C138 (= C135)
- binding pterin cytosine dinucleotide: Q100 (= Q97), C138 (= C135)
7orcB Human aldehyde oxidase in complex with raloxifene (see paper)
29% identity, 84% coverage: 58:456/474 of query aligns to 70:491/1299 of 7orcB
- binding flavin-adenine dinucleotide: P228 (≠ R205), V229 (= V206), I230 (≠ F207), M231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), S235 (≠ D212), V236 (≠ L213), L309 (= L277), A310 (≠ G278), A318 (≠ G286), S319 (≠ T287), G322 (= G290), H323 (≠ N291), S326 (≠ T294), H328 (≠ S296), S331 (≠ G299), D332 (= D300), L376 (≠ I343), L403 (≠ C370)
- binding fe2/s2 (inorganic) cluster: C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
- binding raloxifene: G417 (≠ T384), D418 (≠ G385), G419 (= G386), H449 (≠ R415), N451 (≠ D417)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 43, 44
- binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 716
- binding raloxifene: 506, 507, 616, 619, 740, 743, 746
8emtA Cryo-em analysis of the human aldehyde oxidase from liver (see paper)
28% identity, 84% coverage: 58:456/474 of query aligns to 70:478/1254 of 8emtA
- binding flavin-adenine dinucleotide: P215 (≠ R205), V216 (= V206), I217 (≠ F207), M218 (≠ A208), G219 (= G209), T221 (= T211), S222 (≠ D212), V223 (≠ L213), L296 (= L277), A297 (≠ G278), S306 (≠ T287), G309 (= G290), H310 (≠ N291), I312 (≠ A293), D319 (= D300)
- binding fe2/s2 (inorganic) cluster: C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
Sites not aligning to the query:
8emtB Cryo-em analysis of the human aldehyde oxidase from liver (see paper)
28% identity, 84% coverage: 58:456/474 of query aligns to 68:473/1221 of 8emtB
- binding flavin-adenine dinucleotide: P210 (≠ R205), V211 (= V206), M213 (≠ A208), G214 (= G209), T216 (= T211), S217 (≠ D212), V218 (≠ L213), L291 (= L277), A292 (≠ G278), A300 (≠ G286), G304 (= G290), H305 (≠ N291), I307 (≠ A293), D314 (= D300)
- binding fe2/s2 (inorganic) cluster: C69 (= C59), C109 (= C98), C112 (= C101), C144 (= C133), C146 (= C135)
Sites not aligning to the query:
2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
28% identity, 95% coverage: 5:456/474 of query aligns to 6:491/1291 of 2e3tA
- binding flavin-adenine dinucleotide: G44 (= G42), K228 (≠ R205), L229 (≠ V206), V230 (≠ F207), V231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), E235 (≠ D212), I236 (≠ L213), L309 (= L277), A318 (≠ G286), S319 (≠ T287), G322 (= G290), N323 (= N291), I325 (≠ A293), T326 (= T294), S331 (≠ G299), D332 (= D300), I375 (= I342), L376 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), N69 (vs. gap), C71 (= C59), Q109 (= Q97), C110 (= C98), C113 (= C101), C145 (= C133), C147 (= C135)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 882, 883, 887, 888, 891
- binding calcium ion: 713, 714, 716, 809, 810, 840, 843, 844, 847, 880, 881
- binding fe2/s2 (inorganic) cluster: 717
- binding uric acid: 775, 853, 887, 982, 983, 1051, 1052, 1234
6a7xA Rat xanthine oxidoreductase, d428a variant, NAD bound form
28% identity, 95% coverage: 5:456/474 of query aligns to 6:491/1295 of 6a7xA
- binding flavin-adenine dinucleotide: G44 (= G42), K228 (≠ R205), L229 (≠ V206), V230 (≠ F207), V231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), E235 (≠ D212), I236 (≠ L213), F309 (≠ L277), V314 (≠ I282), S319 (≠ T287), G322 (= G290), N323 (= N291), I325 (≠ A293), T326 (= T294), D332 (= D300), L376 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), N69 (vs. gap), C71 (= C59), Q109 (= Q97), C110 (= C98), G111 (= G99), C113 (= C101), C145 (= C133), C147 (= C135)
- binding nicotinamide-adenine-dinucleotide: P329 (= P297), Y365 (vs. gap), R366 (vs. gap), A432 (≠ G398), D433 (≠ P399), P473 (≠ R439), R480 (= R445)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 883, 884, 887, 888, 891
- binding uric acid: 775, 853, 887, 982, 983, 1052, 1234
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
49% identity, 31% coverage: 3:148/474 of query aligns to 5:150/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ E37), C41 (= C39), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C61 (= C59), C100 (= C98), G101 (= G99), C103 (= C101), C135 (= C133), C137 (= C135)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C137 (= C135)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
49% identity, 31% coverage: 1:145/474 of query aligns to 4:148/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ T41), G45 (= G42), E46 (= E43)
- binding fe2/s2 (inorganic) cluster: E40 (= E37), C42 (= C39), S43 (≠ G40), G45 (= G42), C47 (= C44), G48 (= G45), C50 (= C47), C62 (= C59), Q100 (= Q97), C101 (= C98), G102 (= G99), C104 (= C101), C136 (= C133), C138 (= C135)
- binding pterin cytosine dinucleotide: Q100 (= Q97), C138 (= C135)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
49% identity, 31% coverage: 1:145/474 of query aligns to 4:148/157 of Q0QLF3
- C42 (= C39) binding [2Fe-2S] cluster
- C47 (= C44) binding [2Fe-2S] cluster
- C50 (= C47) binding [2Fe-2S] cluster
- C62 (= C59) binding [2Fe-2S] cluster
- C101 (= C98) binding [2Fe-2S] cluster
- C104 (= C101) binding [2Fe-2S] cluster
- C136 (= C133) binding [2Fe-2S] cluster
- C138 (= C135) binding [2Fe-2S] cluster
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
47% identity, 31% coverage: 1:148/474 of query aligns to 10:156/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C39), S50 (≠ G42), C52 (= C44), G53 (= G45), C55 (= C47), C67 (= C59), C106 (= C98), G107 (= G99), C109 (= C101), C141 (= C133), C143 (= C135)
- binding pterin cytosine dinucleotide: Q105 (= Q97), C143 (= C135)
2ckjA Human milk xanthine oxidoreductase
31% identity, 92% coverage: 2:435/474 of query aligns to 3:444/1264 of 2ckjA
- binding flavin-adenine dinucleotide: L228 (≠ V206), V230 (≠ A208), G231 (= G209), N232 (≠ G210), T233 (= T211), E234 (≠ D212), F308 (≠ L277), A309 (≠ G278), A317 (≠ G286), S318 (≠ T287), G321 (= G290), N322 (= N291), I324 (≠ A293), T325 (= T294), D331 (= D300), L375 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), R147 (= R134), C148 (= C135)
Sites not aligning to the query:
2e1qA Crystal structure of human xanthine oxidoreductase mutant, glu803val (see paper)
30% identity, 92% coverage: 2:435/474 of query aligns to 3:451/1307 of 2e1qA
- binding flavin-adenine dinucleotide: E43 (≠ T41), G44 (= G42), K230 (≠ R205), L231 (≠ V206), V232 (≠ F207), V233 (≠ A208), G234 (= G209), N235 (≠ G210), T236 (= T211), E237 (≠ D212), I238 (≠ L213), F311 (≠ L277), A312 (≠ G278), V316 (≠ I282), A320 (≠ G286), S321 (≠ T287), G324 (= G290), N325 (= N291), I327 (≠ A293), T328 (= T294), S333 (≠ G299), D334 (= D300), I377 (= I342), L378 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), Q110 (= Q97), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
Sites not aligning to the query:
- active site: 742, 777, 855, 859, 887, 1235, 1236
- binding bicarbonate ion: 814, 815, 852, 886, 889, 890, 893
- binding calcium ion: 715, 716, 718, 741, 747, 780, 781, 784, 811, 812
- binding fe2/s2 (inorganic) cluster: 719
- binding hydroxy(dioxo)molybdenum: 773, 774, 887, 1053, 1054, 1236
3amzA Bovine xanthine oxidoreductase urate bound form (see paper)
30% identity, 89% coverage: 5:425/474 of query aligns to 6:440/1291 of 3amzA
- binding flavin-adenine dinucleotide: G44 (= G42), L229 (≠ V206), V230 (≠ F207), V231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), E235 (≠ D212), I236 (≠ L213), F309 (≠ L277), A318 (≠ G286), S319 (≠ T287), G322 (= G290), N323 (= N291), I325 (≠ A293), T326 (= T294), S331 (≠ G299), D332 (= D300), I375 (= I342), L376 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), Q110 (= Q97), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S328 (= S296), P329 (= P297), I330 (≠ A298), Y365 (≠ P332), R366 (≠ G333), D402 (≠ A369), I403 (≠ C370), G430 (≠ R415), A432 (≠ D417), D433 (≠ Q418)
Sites not aligning to the query:
- active site: 731, 766, 844, 848, 876, 1224, 1225
- binding bicarbonate ion: 803, 804, 873, 874, 878, 879, 882
- binding calcium ion: 831, 834, 835, 838, 871, 872
- binding fe2/s2 (inorganic) cluster: 708
- binding 1,4-dihydronicotinamide adenine dinucleotide: 473, 480, 1189
- binding uric acid: 766, 844, 878, 973, 974, 1043, 1225
3amzB Bovine xanthine oxidoreductase urate bound form (see paper)
30% identity, 89% coverage: 5:425/474 of query aligns to 6:440/1289 of 3amzB
- binding flavin-adenine dinucleotide: G44 (= G42), K228 (≠ R205), L229 (≠ V206), V230 (≠ F207), V231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), E235 (≠ D212), I236 (≠ L213), F309 (≠ L277), V314 (≠ I282), A318 (≠ G286), S319 (≠ T287), G322 (= G290), N323 (= N291), I325 (≠ A293), T326 (= T294), S331 (≠ G299), D332 (= D300), L376 (≠ I343)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), N69 (vs. gap), C71 (= C59), Q110 (= Q97), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S328 (= S296), P329 (= P297), I330 (≠ A298), Y365 (≠ P332), R366 (≠ G333), D402 (≠ A369), I403 (≠ C370), G430 (≠ R415), A432 (≠ D417), D433 (≠ Q418)
Sites not aligning to the query:
- active site: 731, 766, 844, 848, 876, 1224, 1225
- binding bicarbonate ion: 803, 804, 873, 874, 878, 879, 882
- binding calcium ion: 831, 834, 835, 838, 871, 872
- binding fe2/s2 (inorganic) cluster: 708
- binding 1,4-dihydronicotinamide adenine dinucleotide: 473, 480, 1189
- binding uric acid: 766, 844, 878, 973, 974, 1043, 1225
Q0QLF4 Nicotinate dehydrogenase FAD-subunit; NDH; Nicotinic acid hydroxylase FAD-subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
31% identity, 58% coverage: 185:459/474 of query aligns to 6:285/296 of Q0QLF4
- 29:36 (vs. 206:213, 75% identical) binding FAD
- G101 (= G278) binding FAD
- TIGGN 110:114 (= TIGGN 287:291) binding FAD
- D123 (= D300) binding FAD
- R160 (≠ Q334) binding FAD
- M169 (≠ I343) binding FAD
- K187 (= K361) binding FAD
3am9A Complex of bovine xanthine dehydrogenase and trihydroxy fyx-051 (see paper)
30% identity, 89% coverage: 5:425/474 of query aligns to 6:440/1293 of 3am9A
- binding flavin-adenine dinucleotide: E43 (≠ T41), G44 (= G42), K228 (≠ R205), L229 (≠ V206), V231 (≠ A208), G232 (= G209), N233 (≠ G210), T234 (= T211), E235 (≠ D212), I236 (≠ L213), F309 (≠ L277), A310 (≠ G278), V314 (≠ I282), A318 (≠ G286), S319 (≠ T287), G322 (= G290), N323 (= N291), I325 (≠ A293), T326 (= T294), D332 (= D300), L376 (≠ I343), K394 (= K361)
- binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), C111 (= C98), G112 (= G99), C114 (= C101), C146 (= C133), C148 (= C135)
Sites not aligning to the query:
- active site: 733, 768, 846, 850, 878, 1226, 1227
- binding bicarbonate ion: 805, 806, 875, 876, 877, 884
- binding calcium ion: 707, 709, 802, 803, 833, 836, 837, 840, 873, 874
- binding fe2/s2 (inorganic) cluster: 710
- binding 4-[5-(2,6-dioxo-1,2,3,6-tetrahydropyridin-4-yl)-1H-1,2,4-triazol-3-yl]-6-oxo-1,6-dihydropyridine-2-carbonitrile: 614, 768, 839, 842, 846, 880, 975, 980, 1045, 1227
Query Sequence
>WP_083453266.1 NCBI__GCF_001293685.1:WP_083453266.1
MKIEFELNGKPICLDAPADRRAVDLLREDLGLTGTKEGCGTGECGACTILVDGRSRLSCL
MLVPQLEGRKITTIEGLAPGEHLHPVQAAFVRHGAVQCGFCTPGMVLTAVDLLARHPEPR
HDEVIEAVSGNLCRCTGYQKIVSAVMDAGNTPPMPCSRQEPAPDPPPNGLRNAGRPGGTT
AGSPVWMPRTLEDLWPLLAQYPQARVFAGGTDLLVWRRNGKIDSPALIGLERIDALRGIA
ETPDTVRIGAATTHEALLADPLVSRHFPVLKQALRTLGSPHIRHVGTIGGNLATASPAGD
TLPPLHVLDATVTVQSAEGTRHLPIASLITGPGQTALMPGEIISAVIIPKPAADARQHFE
KVGLRQAMACAVASLAAVVTFDDTGGIDRARLAWGSVGPTVVRLPEVEAALCGGRLDQKT
LDALRPLVSRHLSPISDVRAGADYRRRVAGNLLLRLADDRPTRQATADTVEQAS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory