SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_083509555.1 NCBI__GCF_001541235.1:WP_083509555.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
59% identity, 99% coverage: 4:476/476 of query aligns to 2:474/474 of P0A9P0

query
sites
P0A9P0

A

S

V

T

D

E

I

I

E

K

A

T

D

Q

I

V

V

V

L

L

G

G

A

A

G

G

P

P

G

A

G

G

Y

Y

T

S

A

A

F

F

R

R

A

C

A

A

D

D

L

L

G

G

K

L

K

E

V

T

V

V

V

I

V

V

E

E

R

R

G

Y

P

N

A

T

L

L

G

G

V

V

C

C

L

L

N

N

V

V

G

G

C

C

I

I

P

P

S

S

K

K

A

A

L

L

H

H

A

V

A

A

K

K

V

V

L

I

E

E

D

E

A

A

D

K

E

A

M

L

T

A

E

E

A

H

G

G

L

I

G

V

F

F

T

G

R

E

P

P

Q

K

L

T

D

D

L

I

D

D

K

K

L

I

R

R

R

T

W

W

K

K

S

E

G

K

I

V

V

I

S

N

K

Q

L

L

T

T

G

G

L

L

A

A

A

G

L

M

A

A

R

K

Q

G

R

R

K

K

V

V

I

K

V

V

T

N

G

G

F

L

G

G

R

K

F

F

V

T

S

G

P

A

N

N

E

T

I

L

E

E

V

V

T

E

H

G

E

E

G

N

A

G

T

K

K

T

R

V

V

I

A

N

F

F

T

D

Q

N

A

A

I

I

A

A

G

G

S

S

E

R

P

P

A

I

R

Q

L

L

P

P

I

F

A

I

P

P

K

H

H

E

D

D

S

P

R

R

I

I

L

W

D

D

S

S

T

T

G

D

A

A

L

L

D

E

L

L

Q

K

D

E

V

V

P

P

P

E

R

R

L

L

V

V

V

M

G

G

I

I

G

G

L

L

E

E

M

M

A

G

T

T

V

V

Y

Y

H

H

A

A

L

L

G

G

S

S

R

Q

V

I

T

D

V

V

E

E

A

M

M

F

D

D

Q

Q

L

V

I

I

S

P

G

A

A

A

D

D

R

K

D

D

I

I

V

V

A
x
K

P

V

L

F

M

T

K

K

R

R

I

I

S

S

T

K

R

K

Y

F

D

N

A

-

V

L

H

M

L

L

G

E

T

T

K

K

V

V

R

T

N

A

I

V

A

E

S

A

A

K

A

E

A

D

E

G

L

I

T

Y

V

V

T

T

F

M

E

E

G

G

A

K

N

K

A

A

P

P

-

A

E

E

E

P

Q

Q

S

R

F

Y

D

D

K

A

I

V

L

L

V

V

A

A

V

I

G

G

R

R

V

V

P

P

N

N

S

G

G

K

T

N

L

L

G

D

L

A

A

G

A

K

A

A

G

G

V

V

A

E

V

V

D

D

Q

D

R

R

G

G

F

F

I

I

R

R

V

V

D

D

K

K

Q

Q

M

L

R

R

T

T

N

N

V

V

P

P

H

H

I

I

F

F

A

A

I

I

G

G

D

D

V

I

V

V

G

G

Q

Q

P

P

M

M

L

L

A

A

H

H

K

K

A

G

V

V

H

H

E

E

G

G

K

H

V

V

A

A

E

E

V

V

A

I

A

A

G

G

L

K

K

K

A

H

A

Y

F

F

D

D

A

P

R

K

V

V

I

I

P

P

S

S

V

I

A

A

Y

Y

T

T

D

E

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

L

T

T

E

E

A

K

E

E

A

A

N

K

A

E

N

K

G

G

V

I

A

S

Y

Y

G

E

K

T

G

A

S

T

F

F

P

P

W

W

E

A

A

A

S

S

G

G

R

R

A

A

L

I

S

A

Q

S

G

D

R

C

S

A

E

D

G

G

L

M

T

T

K

K

V

L

L

I

F

F

D

D

P

K

E

E

T

S

H

H

R

R

T

V

L

I

G

G

C

G

G

A

I

I

V

V

G

G

A

T

H

N

A

G

G

G

E

E

L

L

I

L

G

G

E

E

M

I

V

G

L

L

A

A

I

I

E

E

M

M

G

G

A

C

D

D

A

A

A

E

D

D

I

I

A

A

G

L

T

T

I

I

H

H

P

A

H

H

P

P

T

T

L

L

S

H

E

E

T

S

I

V

A

G

Q

L

S

A

A

A

E

E

M

V

F

F

E

E

G

G

T

S

I

I

T

T

D

D

L

L

I

P

A

N

R

P

Q

K

A

A

R

K

Q

K

R

K

K220 (≠ A222) modified: N6-acetyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
59% identity, 99% coverage: 4:474/476 of query aligns to 1:471/471 of 4jdrA

query
sites
4jdrA

A

S

V

T

D

E

I

I

E

K

A

T

D

Q

I

V

V

V

L

L

G
|
G

A

A

G
|
G

P
|
P

G
x
A

G

G

Y

Y

T

S

A

A

F

F

R

R

A

C

A

A

D

D

L

L

G

G

K

L

K

E

V

T

V

V

V

I

V

V

E
|
E

R
|
R

G
x
Y

P

N

A

T

L
|
L

G

G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

H

A

V

A

A

K

K

V

V

L

I

E

E

D

E

A

A

D

K

E

A

M

L

T

A

E

E

A

H

G

G

L

I

G

V

F

F

T

G

R
x
E

P
|
P

Q

K

L

T

D

D

L

I

D

D

K

K

L

I

R

R

R

T

W

W

K

K

S

E

G

K

I

V

V

I

S

N

K

Q

L

L

T

T

G

G

L

L

A

A

A

G

L

M

A

A

R

K

Q

G

R

R

K

K

V

V

I

K

V

V

T

N

G

G

F
x
L

G
|
G

R

K

F

F

V

T

S

G

P

A

N

N

E

T

I

L

E

E

V

V

T

E

H

G

E

E

G

N

A

G

T

K

K

T

R

V

V

I

A

N

F

F

T

D

Q

N

A

A

I

I

A

A

A
|
A

G
|
G

S

S

E

R

P

P

A

I

R

Q

L

L

P

P

I

F

A

I

P

P

K

H

H

E

D

D

S

P

R

R

I

I

L

W

D

D

S

S

T

T

G

D

A

A

L

L

D

E

L

L

Q

K

D

E

V

V

P

P

P

E

R

R

L

L

V

V

V

M

G

G

I
|
I

G

G

L

L

E
|
E

M

M

A

G

T

T

V

V

Y

Y

H

H

A

A

L

L

G

G

S

S

R

Q

V

I

T

D

V

V

E

E

A

M

M

F

D

D

Q

Q

L

V

I

I

S

P

G

A

A

A

D

D

R

K

D

D

I

I

V

V

A

K

P

V

L

F

M

T

K

K

R

R

I

I

S

S

T

K

R

K

Y

F

D

N

A

-

V

L

H

M

L

L

G

E

T

T

K

K

V

V

R

T

N

A

I

V

A

E

S

A

A

K

A

E

A

D

E

G

L

I

T

Y

V

V

T

T

F

M

E

E

G

G

A

K

N

K

A

A

P

P

-

A

E

E

E

P

Q

Q

S

R

F

Y

D

D

K

A

I

V

L

L

V

V

A

A

V

I

G

G

R

R

V

V

P

P

N

N

S

G

G

K

T

N

L

L

G

D

L

A

A

G

A

K

A

A

G

G

V

V

A

E

V

V

D

D

Q

D

R

R

G

G

F

F

I

I

R

R

V

V

D

D

K

K

Q

Q

M

L

R

R

T

T

N

N

V

V

P

P

H

H

I

I

F

F

A

A

I

I

G
|
G

D
|
D

V

I

V

V

G

G

Q

Q

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

G

V
|
V

H

H

E

E

G

G

K

H

V

V

A

A

E

E

V

V

A

I

A

A

G

G

L

K

K

K

A

H

A

Y

F

F

D

D

A

P

R

K

V

V

I

I

P

P

S

S

V

I

A

A

Y

Y

T

T

D

E

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

L

T

T

E

E

A

K

E

E

A

A

N

K

A

E

N

K

G

G

V

I

A

S

Y

Y

G

E

K

T

G

A

S

T

F

F

P

P

W

W

E

A

A

A

S

S

G

G

R

R

A

A

L

I

S

A

Q

S

G

D

R

C

S

A

E

D

G

G

L

M

T

T

K

K

V

L

L

I

F

F

D

D

P

K

E

E

T

S

H

H

R

R

T

V

L

I

G

G

C

G

G

A

I

I

V

V

G

G

A

T

H

N

A

G

G

G

E

E

L

L

I

L

G

G

E

E

M

I

V

G

L

L

A

A

I

I

E

E

M

M

G

G

A

C

D

D

A

A

A

E

D

D

I

I

A

A

G

L

T

T

I

I

H
|
H

P

A

H
|
H

P

P

T

T

L

L

S

H

E
|
E

T

S

I

V

A

G

Q

L

S

A

A

A

E

E

M

V

F

F

E

E

G

G

T

S

I

I

T

T

D

D

L

L

I

P

A
x
N

R
x
P

Q

K

A

A

R

K

active site: P15 (= P18), L40 (= L43), C44 (= C47), C49 (= C52), S52 (= S55), E77 (≠ R80), P78 (= P81), I184 (= I187), E188 (= E191), V324 (= V327), H442 (= H445), H444 (= H447), E449 (= E452), N467 (≠ A470), P468 (≠ R471)
binding flavin-adenine dinucleotide: G12 (= G15), G14 (= G17), P15 (= P18), A16 (≠ G19), E35 (= E38), R36 (= R39), Y37 (≠ G40), V43 (= V46), C44 (= C47), G48 (= G51), C49 (= C52), K53 (= K56), L115 (≠ F118), G116 (= G119), A144 (= A147), G145 (= G148), I185 (= I188), G311 (= G314), D312 (= D315), M318 (= M321), L319 (= L322), A320 (= A323), H321 (= H324)

1bhyA Low temperature middle resolution structure of p64k from masc data (see paper)
58% identity, 98% coverage: 4:468/476 of query aligns to 2:477/482 of 1bhyA

query
sites
1bhyA

A

S

V

A

D

D

I

A

E

E

A

Y

D

D

I

V

V

V

L
|
L

G

G

A

G

G

G

P
|
P

G
|
G

G

G

Y

Y

T

S

A

A

F

F

R

A

A

A

D

D

L

E

G

G

K

L

K

K

V

V

V

A

V

I

V

V

E
|
E

R
|
R

G
x
Y

P

K

A

T

L
|
L

G

G

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

H

A

N

A

A

K

A

V

V

L

I

E

D

D

E

A

V

D

R

E

H

M

L

T

A

E

A

A

N

G

G

L

I

G

K

F

Y

T

P

R

E

P

P

Q

E

L

L

D

D

L

I

D

D

K

M

L

L

R

R

R

A

W

Y

K

K

S

D

G

G

I

V

V

V

S

S

K

R

L

L

T

T

G

G

L

L

A

A

A

G

L

M

A

A

R

K

Q

S

R

R

K

K

V

V

I

D

V

V

V

I

T

Q

G

G

F
x
D

G
|
G

R

Q

F

F

V

L

S

D

P

P

N

H

E

H

I

L

E

E

V

V

T

S

-

L

-

T

-

A

-

G

-

D

-

A

H
x
Y

E

E

-

Q

-

A

-

P

-

T

G

G

A

E

T

K

K

K

R

I

V

V

A

A

F

F

T

K

Q

N

A

C

I

I

A

A

A
|
A

G
|
G

S

S

E

R

P

V

A

T

R

K

L

L

P

P

I

F

A

I

P

P

K

E

H

-

D

D

S

P

R

R

I

I

L

I

D

D

S

S

T

S

G

G

A

A

L

L

D

A

L

L

Q

K

D

E

V

V

P

P

P

G

R

K

L

L

V

I

G

G

I
|
I

I

I

G

G

L

L

E
|
E

M

M

A

G

T

T

V

V

Y

Y

H

S

A

T

L

L

G

G

S

S

R

R

V

L

T

D

V

V

E

E

A

M

M

M

D

D

Q

G

L

L

I

M

S

Q

G

G

A

A

D

D

R

R

D

D

I

L

V

V

A

K

P

V

L

W

M

Q

K

K

R

Q

I

N

S

E

T

Y

R

R

Y

F

D

D

A

N

V

I

H

M

L

V

G

N

T

T

K

K

V

T

R

V

N

A

I

V

A

E

S

P

A

K

A

E

A

D

E

G

L

V

T

Y

V

V

T

T

F

F

E

E

G

G

A

A

N

N

A

A

P

P

E

K

E

E

-

P

Q

Q

S

R

F

Y

D

D

K

A

I

V

L

L

V

V

A

A

V

A

G

G

R

R

V

A

P

P

N

N

S

G

G

K

T

L

L

I

G

S

L

A

A

E

A

K

A

A

G

G

V

V

A

A

V

V

D

T

Q

D

R

R

G

G

F

F

I

I

R

E

V

V

D

D

K

K

Q

Q

M

M

R

R

T

T

N

N

V

V

P

P

H

H

I

I

F

Y

A

A

I

I

G

G

D
|
D

V

I

V

V

G

G

Q

Q

P

P

M

M

L
|
L

A
|
A

H

H

K

K

A

A

V

V

H

H

E

E

G

G

K

H

V

V

A

A

E

E

V

N

A

C

A

A

G

G

L

H

K

K

A

A

A

Y

F

F

D

D

A

A

R

R

V

V

I

I

P

P

S

G

V

V

A

A

Y

Y

T

T

D

S

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

E

T

T

E

E

A

L

E

S

A

A

N

K

A

A

N

S

G

A

V

R

A

K

Y

I

G

T

K

K

G

A

S

N

F

F

P

P

W

W

E

A

A

A

S

S

G

G

R

R

A

A

L

I

S

A

Q

N

G

G

R

C

S

D

E

K

G

P

L

F

T

T

K

K

V

L

L

I

F

F

D

D

P

A

E

E

T

T

H

G

R

R

T

I

L

I

G

G

C

G

G

G

I

I

V

V

G

G

A

P

H

N

A

G

G

G

E

D

L

M

I

I

G

G

E

E

M

V

V

Y

L

L

A

A

I

I

E

E

M

M

G

G

A

C

D

D

A

A

D

D

I

I

A

G

G

K

T

T

I

I

H
|
H

P

P

H
|
H

P

P

T

T

L

L

S

G

E
|
E

T

S

I

I

A

G

Q

M

S

A

A

A

E

E

M

V

F

A

E

L

G

G

T

T

I

C

T

T

D

D

L

L

active site: L41 (= L43), C45 (= C47), C50 (= C52), S53 (= S55), I195 (= I187), E199 (= E191), H454 (= H445), H456 (= H447), E461 (= E452)
binding flavin-adenine dinucleotide: L12 (= L14), P16 (= P18), G17 (= G19), E36 (= E38), R37 (= R39), Y38 (≠ G40), G43 (= G45), V44 (= V46), C45 (= C47), G49 (= G51), C50 (= C52), K54 (= K56), D116 (≠ F118), G117 (= G119), Y135 (≠ H131), A156 (= A147), G157 (= G148), D324 (= D315), L331 (= L322), A332 (= A323)

Sites not aligning to the query:

active site: 479, 480

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
44% identity, 98% coverage: 7:471/476 of query aligns to 8:470/470 of P11959

query
sites
P11959

I

I

E

E

A

T

D

E

I

T

V

L

V

V

L

V

G

G

A

A

G

G

P

P

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

Q

K

K

V

V

V

T

V

I

V

V

E
|
E

R
x
K

G
|
G

P
x
N

A

-

L
|
L

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G

G

C

C

I

I

P

P

S

S

K
|
K

A

A

L

L

L

I

H

S

A

A

A

S

K

H

V

R

L

Y

E

E

D

Q

A

A

D

K

E

H

M

S

T

E

E

E

A

M

G

G

L

I

G

K

F

A

T

E

R

N

P

V

Q

T

L

I

D

D

L

F

D

A

K

K

L

V

R

Q

R

E

W

W

K

K

S

A

G

S

I

V

V

V

S

K

K

K

L

L

T

T

G

G

L

V

A

E

A

G

L

L

A

L

R

K

Q

G

R

N

K

K

V

V

I

E

V

I

V

V

T

K

G

G

F

E

G

A

R

Y

F

F

V

V

S

D

P

A

N

N

E

T

I

V

E

R

V

V

T

V

H

N

E

G

G

D

A

S

T

A

K

Q

R

T

V

Y

A

T

F

F

T

K

Q

N

A

A

I

I

A

A

A

T

G

G

S

S

E

R

P

P

A

I

R

E

L

L

P

P

I

-

A

N

P

F

K

K

H

F

D

S

S

N

R

R

I

I

L

L

D

D

S

S

T

T

G

G

A

A

L

L

D

N

L

L

Q

G

D

E

V

V

P

P

P

K

R

S

L

L

L

V

V

V

V

I

G

G

I

Y

I

I

G

G

L

I

E

E

M

L

A

G

T

T

V

A

Y

Y

H

A

A

N

L

F

G

G

S

T

R

K

V

V

T

T

V

I

V

L

E

E

A

G

M

A

D

G

Q

E

L

I

I

L

S

S

G

G

A

F

D

E

R

K

D

Q

I

M

V

A

A

A

P

I

L

I

M

K

K

K

R

R

I

L

S

K

T

K

R

K

Y

G

D

V

A

E

V

V

H

-

L

-

G

-

T

-

K

-

V

V

R

T

N

N

I

A

A

L

S

A

A

K

A

G

A

A

E

E

-

E

-

R

-

E

-

D

-

G

L

V

T

T

V

V

T

T

F

Y

E

E

G

A

A

N

N

G

A

E

P

T

E

K

E

T

Q

I

S

D

F

A

D

D

K

Y

I

V

L

L

V

V

A

T

V

V

G

G

R

R

V

R

P

P

N

N

S

T

G

D

T

E

L

L

G

G

L

L

A

E

A

Q

A

I

G

G

V

I

A

K

V

M

D

T

Q

N

R

R

G

G

F

L

I

I

R

E

V

V

D

D

K

Q

Q

Q

M

C

R

R

T

T

N

S

V

V

P

P

H

N

I

I

F

F

A

A

I

I

G

G

D
|
D

V

I

V

V

G

P

Q

G

P

P

M

A

L

L

A
|
A

H

H

K

K

A

A

V

S

H

Y

E

E

G

G

K

K

V

V

A

A

E

E

V

A

A

I

A

A

G

G

L

H

K

P

A

S

A

A

F

V

D

D

A

Y

R

V

V

A

I

I

P

P

S

A

V

V

A

V

Y

F

T

S

D

D

P

P

E

E

V

C

A

A

W

S

V

V

G

G

L

Y

T

F

E

E

A

Q

E

Q

A

A

N

K

A

D

N

E

G

G

V

I

A

D

Y

V

G

I

K

A

G

A

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

G

R

R

A

A

L

L

S

A

Q

L

G

N

R

D

S

T

E

D

G

G

L

F

T

L

K

K

V

L

L

V

F

V

D

R

P

K

E

E

T

D

H

G

R

V

T

I

L

I

G

G

C

A

G

Q

I

I

V

I

G

G

A

P

H

N

A

A

G

S

E

D

L

M

I

I

G

A

E

E

M

L

V

G

L

L

A

A

I

I

E

E

M

A

G

G

A

M

D

T

A

A

A

E

D

D

I

I

A

A

G

L

T

T

I

I

H

H

P

A

H

H

P

P

T

T

L

L

S

G

E

E

T

I

I

A

A

M

Q

E

S

A

A

A

E

E

M

V

F

A

E

L

G

G

T

T

I

P

T

I

D

H

L

I

I

I

A

T

R

K

39:47 (vs. 38:47, 70% identical) binding FAD
K56 (= K56) binding FAD
D314 (= D315) binding FAD
A322 (= A323) binding FAD

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
45% identity, 95% coverage: 7:460/476 of query aligns to 2:453/455 of 1ebdA

query
sites
1ebdA

I

I

E

E

A

T

D

E

I

T

V

L

V

V

L

V

G
|
G

A

A

G
|
G

P
|
P

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

Q

K

K

V

V

V

T

V

I

V
|
V

E
|
E

R
x
K

G

G

P

N

A

-

L
|
L

G

G

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

I

H

S

A

A

A

S

K

H

V

R

L

Y

E

E

D

Q

A

A

D

K

E

H

M

S

T

E

E

E

A

M

G

G

L

I

G

K

F

A

T

E

R
x
N

P
x
V

Q

T

L

I

D

D

L

F

D

A

K

K

L

V

R

Q

R

E

W

W

K

K

S

A

G

S

I

V

V

V

S

K

K

K

L

L

T

T

G

G

L

V

A

E

A

G

L

L

A

L

R

K

Q

G

R

N

K

K

V

V

I

E

V

I

V

V

T

K

G

G

F
x
E

G
x
A

R

Y

F

F

V

V

S

D

P

A

N

N

E

T

I

V

E

R

V

V

T

V

H

N

E

G

G

D

A

S

T

A

K

Q

R

T

V

Y

A

T

F

F

T

K

Q

N

A

A

I

I

A

A

A
x
T

G
|
G

S

S

E

R

P

P

A

I

R

E

L

L

P

P

I

-

A

N

P

F

K

K

H

F

D

S

S

N

R

R

I

I

L

L

D

D

S

S

T

T

G

G

A

A

L

L

D

N

L

L

Q

G

D

E

V

V

P

P

P

K

R

S

L

L

L

V

V

V

V

I

G

G

I
x
Y

I
|
I

G

G

L

I

E
|
E

M

L

A

G

T

T

V

A

Y

Y

H

A

A

N

L

F

G

G

S

T

R

K

V

V

T

T

V

I

V

L

E

E

A

G

M

A

D

G

Q

E

L

I

I

L

S

S

G

G

A

F

D

E

R

K

D

Q

I

M

V

A

A

A

P

I

L

I

M

K

K

K

R

R

I

L

S

K

T

K

R

K

Y

G

D

V

A

E

V

V

H

-

L

-

G

-

T

-

K

-

V

V

R

T

N

N

I

A

A

L

S

A

A

K

A

G

A

A

E

E

-

E

-

R

-

E

-

D

-

G

L

V

T

T

V

V

T

T

F

Y

E

E

G

A

A

N

N

G

A

E

P

T

E

K

E

T

Q

I

S

D

F

A

D

D

K

Y

I

V

L

L

V

V

A

T

V

V

G

G

R
|
R

V

R

P

P

N

N

S

T

G

D

T

E

L

L

G

G

L

L

A

E

A

Q

A

I

G

G

V

I

A

K

V

M

D

T

Q

N

R

R

G

G

F

L

I

I

R

E

V

V

D

D

K

Q

Q

Q

M

C

R

R

T

T

N

S

V

V

P

P

H

N

I

I

F

F

A

A

I

I

G

G

D
|
D

V

I

V

V

G

P

Q

G

P

P

M
x
A

L
|
L

A
|
A

H

H

K

K

A

A

V
x
S

H

Y

E

E

G

G

K

K

V

V

A

A

E

E

V

A

A

I

A

A

G

G

L

H

K

P

A

S

A

A

F

V

D

D

A

Y

R

V

V

A

I

I

P

P

S

A

V

V

A

V

Y

F

T

S

D

D

P

P

E

E

V

C

A

A

W

S

V

V

G

G

L

Y

T

F

E

E

A

Q

E

Q

A

A

N

K

A

D

N

E

G

G

V

I

A

D

Y

V

G

I

K

A

G

A

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

G

R

R

A

A

L

L

S

A

Q

L

G

N

R

D

S

T

E

D

G

G

L

F

T

L

K

K

V

L

L

V

F

V

D

R

P

K

E

E

T

D

H

G

R

V

T

I

L

I

G

G

C

A

G

Q

I

I

V

I

G

G

A

P

H

N

A

A

G

S

E

D

L

M

I

I

G

A

E

E

M

L

V

G

L

L

A

A

I

I

E

E

M

A

G

G

A

M

D

T

A

A

A

E

D

D

I

I

A

A

G

L

T

T

I

I

H
|
H

P

A

H
|
H

P

P

T

T

L

L

S

G

E
|
E

T

I

I

A

A

M

Q

E

S

A

A

A

E

E

M

V

active site: P13 (= P18), L37 (= L43), C41 (= C47), C46 (= C52), S49 (= S55), N74 (≠ R80), V75 (≠ P81), Y180 (≠ I187), E184 (= E191), S320 (≠ V327), H438 (= H445), H440 (= H447), E445 (= E452)
binding flavin-adenine dinucleotide: G10 (= G15), G12 (= G17), P13 (= P18), V32 (= V37), E33 (= E38), K34 (≠ R39), G39 (= G45), V40 (= V46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), E112 (≠ F118), A113 (≠ G119), T141 (≠ A147), G142 (= G148), Y180 (≠ I187), I181 (= I188), R268 (= R275), D308 (= D315), A314 (≠ M321), L315 (= L322), A316 (= A323)

P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
43% identity, 98% coverage: 10:474/476 of query aligns to 6:478/478 of P14218

query
sites
P14218

D

D

I

V

V

V

L

I

G

G

A

A

G

G

P

P

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

C

V

I

E
|
E

R
x
K

-
x
Y

-
x
I

-
x
G

-
x
K

-
x
E

-
x
G

G
x
K

P
x
V

A
|
A

L
|
L

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

H

D

A

S

K

Y

V

K

L

Y

E

H

D

E

A

A

D

K

E

E

M

A

T

F

E

K

A

V

-

H

G

G

L

I

G

E

F

A

T

K

R

G

P

V

Q

T

L

I

D

D

L

V

D

P

K

A

L

M

R

V

R

A

W

R

K

K

S

A

G

N

I

I

V

V

S

K

K

N

L

L

T

T

G

G

L

I

A

A

A

T

L

L

A

F

R

K

Q

A

R

N

K

G

V

V

I

T

V

S

V

F

T

E

G

G

F

H

G
|
G

R

K

F

L

V

L

S

A

P

N

N

K

E

Q

I

V

E

E

V

V

T

T

H

G

-

L

E

D

G

G

A

K

T

T

K

Q

R

V

V

L

A

E

F

A

T

E

Q

N

A

V

I

I

A

A

A

S

G

G

S

S

E

R

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

L

H

S

D

D

S

D

R

I

I

I

L

V

D

D

S

S

T

T

G

G

A

A

L

L

D

E

L

F

Q

Q

D

A

V

V

P

P

P

K

R

K

L

L

L

G

V

V

V

I

G

G

G

A

G

G

I

V

I

I

G

G

L

L

E

E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E

E

A

A

M

L

D

D

Q

K

L

F

I

L

S

P

G

A

A

A

D

D

R

E

D

Q

I

I

V

A

A

K

P

E

L

A

M

L

K

K

R

V

I

L

S

T

T

K

R

Q

Y

G

D

L

A

N

V

I

H

R

L

L

G

G

T

A

K

R

V

V

R

T

N

A

I

S

A

E

S

V

A

K

E

Q

L

V

T

T

V

V

T

T

F

F

E

T

G

D

A

A

N

N

A

G

P

E

E

Q

E

K

Q

E

S

T

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V

V

G

G

R

R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

H

M

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

F

F

A

A

I

I

G

G

D
|
D

V

V

G

R

Q

G

P

A

M

M

L

L

A
|
A

H

H

K

K

A

A

V

S

H

E

E

E

G

G

K

V

V

M

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

T

A

L

N

K

A

A

N

E

G

G

V

V

A

E

Y

V

G

N

K

V

G

G

S

T

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

T

G

G

L

L

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

G

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H

F

P

S

H

H

P

P

T

T

L

L

S

S

E

E

T

A

I

L

A

H

Q

E

S

A

A

A

E

L

M

A

F

V

E

N

G

G

T

H

I

A

T

I

D

H

L

I

I

A

A

N

R

R

Q

K

A

K

R

R

34:49 (vs. 38:47, 38% identical) binding FAD
C49 (= C47) modified: Disulfide link with 54, Redox-active
C54 (= C52) modified: Disulfide link with 49, Redox-active
K58 (= K56) binding FAD
G122 (= G119) binding FAD
D319 (= D315) binding FAD
A327 (= A323) binding FAD

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
44% identity, 95% coverage: 10:463/476 of query aligns to 4:465/472 of 5u8vA

query
sites
5u8vA

D

D

I

V

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

C

V

I

E
|
E

R

K

-

Y

-

I

-

G

-

K

-

E

-

G

G

K

P

V

A

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

A

S

K

Y

V

K

L

Y

E

H

D

E

A

A

D

K

E

E

M

A

T

F

E

K

A

V

-

H

G

G

L

I

G

E

F

A

T

K

R
x
G

P
x
V

Q

T

L

I

D

D

L

V

D

P

K

A

L

M

R

V

R

A

W

R

K

K

S

A

G

N

I

I

V

V

S

K

K

N

L

L

T

T

G

G

L

I

A

A

A

T

L

L

A

F

R

K

Q

A

R

N

K

G

V

V

I

T

V

S

V

F

T

E

G

G

F
x
H

G
|
G

R

K

F

L

V

L

S

A

P

N

N

K

E

Q

I

V

E

E

V

V

T

T

H

G

-

L

E

D

G

G

A

K

T

T

K

Q

R

V

V

L

A

E

F

A

T

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

E

R

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

L

H

T

D

D

S

D

R

I

I

I

L

V

D

D

S
|
S

T

T

G

G

A

A

L

L

D

E

L

F

Q

Q

D

A

V

V

P

P

P

K

R

K

L

L

L

G

V

V

V
x
I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V
x
L

E
|
E

A
|
A

M

L

D

D

Q

K

L

F

I

L

S

P

G

A

A

A

D

D

R

E

D

Q

I

I

V

A

A

K

P

E

L

A

M

L

K

K

R

V

I

L

S

T

T

K

R

Q

Y

G

D

L

A

N

V

I

H

R

L

L

G

G

T

A

K

R

V
|
V

R

T

N

A

I

S

A

E

S

V

A

K

E

Q

L

V

T

T

V

V

T

T

F

F

E

T

G

D

A

A

N

N

A

G

P

E

E

Q

E

K

Q

E

S

T

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V
|
V

G
|
G

R
|
R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

H

M

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

F

F

A

A

I

I

G
|
G

D
|
D

V

V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V
x
S

H

E

E

E

G

G

K

V

V

M

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

T

A

L

N

K

A

A

N

E

G

G

V

V

A

E

Y

V

G

N

K

V

G

G

S

T

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

T

G

G

L

L

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

G

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

P

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

T

A

I

L

A

H

Q

E

S

A

A

A

E

L

M

A

F

V

E

N

G

G

active site: P12 (= P18), L43 (= L43), C47 (= C47), C52 (= C52), S55 (= S55), G81 (≠ R80), V82 (≠ P81), V189 (≠ I187), E193 (= E191), S329 (≠ V327), F447 (≠ H445), H449 (= H447), E454 (= E452)
binding flavin-adenine dinucleotide: I8 (≠ L14), G11 (= G17), P12 (= P18), G13 (= G19), E32 (= E38), G45 (= G45), T46 (≠ V46), C47 (= C47), G51 (= G51), C52 (= C52), K56 (= K56), H119 (≠ F118), G120 (= G119), A148 (= A146), S149 (≠ A147), G150 (= G148), S169 (= S167), I190 (= I188), R277 (= R275), G316 (= G314), D317 (= D315), M323 (= M321), L324 (= L322), A325 (= A323), H326 (= H324), H449 (= H447), P450 (= P448)
binding nicotinamide-adenine-dinucleotide: I185 (≠ V183), G186 (= G184), G188 (= G186), V189 (≠ I187), I190 (= I188), L208 (≠ V206), E209 (= E207), A210 (= A208), V243 (= V241), V275 (= V273), G276 (= G274)

Sites not aligning to the query:

active site: 472

5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
44% identity, 95% coverage: 10:463/476 of query aligns to 5:466/473 of 5u8wA

query
sites
5u8wA

D

D

I

V

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

C

V

I

E
|
E

R
x
K

-

Y

-

I

-

G

-

K

-

E

-

G

G

K

P

V

A

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

A

S

K

Y

V

K

L

Y

E

H

D

E

A

A

D

K

E

E

M

A

T

F

E

K

A

V

-

H

G

G

L

I

G

E

F

A

T

K

R
x
G

P
x
V

Q

T

L

I

D

D

L

V

D

P

K

A

L

M

R

V

R

A

W

R

K

K

S

A

G

N

I

I

V

V

S

K

K

N

L

L

T

T

G

G

L

I

A

A

A

T

L

L

A

F

R

K

Q

A

R

N

K

G

V

V

I

T

V

S

V

F

T

E

G

G

F
x
H

G
|
G

R

K

F

L

V

L

S

A

P

N

N

K

E

Q

I

V

E

E

V

V

T

T

H

G

-

L

E

D

G

G

A

K

T

T

K

Q

R

V

V

L

A

E

F

A

T

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

E

R

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

L

H

T

D

D

S

D

R

I

I

I

L

V

D

D

S
|
S

T

T

G

G

A

A

L

L

D

E

L

F

Q

Q

D

A

V

V

P

P

P

K

R

K

L

L

L

G

V

V

V
x
I

G

G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E
|
E

A
|
A

M
x
L

D

D

Q

K

L

F

I

L

S

P

G

A

A

A

D

D

R

E

D

Q

I

I

V

A

A

K

P

E

L

A

M

L

K

K

R

V

I

L

S

T

T

K

R

Q

Y

G

D

L

A

N

V

I

H

R

L

L

G

G

T

A

K

R

V

V

R

T

N

A

I

S

A

E

S

V

A

K

E

Q

L

V

T

T

V

V

T

T

F

F

E

T

G

D

A

A

N

N

A

G

P

E

E

Q

E

K

Q

E

S

T

F

F

D

D

K

K

I

L

L

I

V

V

A
|
A

V
|
V

G
|
G

R
|
R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

H

M

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

F

F

A

A

I

I

G
|
G

D
|
D

V

V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V
x
S

H

E

E

E

G

G

K

V

V

M

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

S

V
|
V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

T

A

L

N

K

A

A

N

E

G

G

V

V

A

E

Y

V

G

N

K

V

G

G

S

T

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

T

G

G

L

L

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

G

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

P

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

T

A

I

L

A

H

Q

E

S

A

A

A

E

L

M

A

F

V

E

N

G

G

active site: P13 (= P18), L44 (= L43), C48 (= C47), C53 (= C52), S56 (= S55), G82 (≠ R80), V83 (≠ P81), V190 (≠ I187), E194 (= E191), S330 (≠ V327), F448 (≠ H445), H450 (= H447), E455 (= E452)
binding flavin-adenine dinucleotide: I9 (≠ L14), G12 (= G17), P13 (= P18), G14 (= G19), E33 (= E38), K34 (≠ R39), G46 (= G45), T47 (≠ V46), C48 (= C47), G52 (= G51), C53 (= C52), K57 (= K56), H120 (≠ F118), G121 (= G119), A149 (= A146), S150 (≠ A147), G151 (= G148), S170 (= S167), G317 (= G314), D318 (= D315), M324 (= M321), L325 (= L322), A326 (= A323), H327 (= H324), Y357 (= Y354), H450 (= H447), P451 (= P448)
binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (≠ V183), G189 (= G186), V190 (≠ I187), I191 (= I188), E194 (= E191), E210 (= E207), A211 (= A208), L212 (≠ M209), A275 (= A272), V276 (= V273), G277 (= G274), R278 (= R275), M324 (= M321), L325 (= L322), V355 (= V352), Y357 (= Y354)

Sites not aligning to the query:

active site: 473

5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
44% identity, 95% coverage: 10:463/476 of query aligns to 8:469/477 of 5u8uD

query
sites
5u8uD

D

D

I

V

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

C

V

I

E
|
E

R
x
K

-

Y

-

I

-

G

-

K

-

E

-

G

G

K

P

V

A

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

A

S

K

Y

V

K

L

Y

E

H

D

E

A

A

D

K

E

E

M

A

T

F

E

K

A

V

-

H

G

G

L

I

G

E

F

A

T

K

R
x
G

P
x
V

Q

T

L

I

D

D

L

V

D

P

K

A

L

M

R

V

R

A

W

R

K

K

S

A

G

N

I

I

V

V

S

K

K

N

L

L

T

T

G

G

L

I

A

A

A

T

L

L

A

F

R

K

Q

A

R

N

K

G

V

V

I

T

V

S

V

F

T

E

G

G

F
x
H

G
|
G

R

K

F

L

V

L

S

A

P

N

N

K

E

Q

I

V

E

E

V

V

T

T

H

G

-

L

E

D

G

G

A

K

T

T

K

Q

R

V

V

L

A

E

F

A

T

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

E

R

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

L

H

T

D

D

S

D

R

I

I

I

L

V

D

D

S

S

T

T

G

G

A

A

L

L

D

E

L

F

Q

Q

D

A

V

V

P

P

P

K

R

K

L

L

L

G

V

V

V

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E

E

A

A

M

L

D

D

Q

K

L

F

I

L

S

P

G

A

A

A

D

D

R

E

D

Q

I

I

V

A

A

K

P

E

L

A

M

L

K

K

R

V

I

L

S

T

T

K

R

Q

Y

G

D

L

A

N

V

I

H

R

L

L

G

G

T

A

K

R

V

V

R

T

N

A

I

S

A

E

S

V

A

K

E

Q

L

V

T

T

V

V

T

T

F

F

E

T

G

D

A

A

N

N

A

G

P

E

E

Q

E

K

Q

E

S

T

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V

V

G

G

R
|
R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

H

M

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

F

F

A

A

I

I

G
|
G

D
|
D

V

V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V
x
S

H

E

E

E

G

G

K

V

V

M

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

T

A

L

N

K

A

A

N

E

G

G

V

V

A

E

Y

V

G

N

K

V

G

G

S

T

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

T

G

G

L

L

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

G

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

P

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

T

A

I

L

A

H

Q

E

S

A

A

A

E

L

M

A

F

V

E

N

G

G

active site: P16 (= P18), L47 (= L43), C51 (= C47), C56 (= C52), S59 (= S55), G85 (≠ R80), V86 (≠ P81), V193 (≠ I187), E197 (= E191), S333 (≠ V327), F451 (≠ H445), H453 (= H447), E458 (= E452)
binding flavin-adenine dinucleotide: I12 (≠ L14), G15 (= G17), P16 (= P18), G17 (= G19), E36 (= E38), K37 (≠ R39), G49 (= G45), T50 (≠ V46), C51 (= C47), G55 (= G51), C56 (= C52), K60 (= K56), H123 (≠ F118), G124 (= G119), A152 (= A146), S153 (≠ A147), G154 (= G148), I194 (= I188), R281 (= R275), G320 (= G314), D321 (= D315), M327 (= M321), L328 (= L322), A329 (= A323), H330 (= H324), H453 (= H447), P454 (= P448)

Sites not aligning to the query:

active site: 476, 477

2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
48% identity, 95% coverage: 10:461/476 of query aligns to 5:451/460 of 2eq6A

query
sites
2eq6A

D

D

I

L

V

I

V

V

L
x
I

G
|
G

A

T

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

H

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

L

K

K

V

V

V

L

V

A

V

V

E
|
E

R
x
A

G

G

P

-

A

E

L
x
V

G

G

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
x
T

K
|
K

A

A

L

L

H

H

A

A

K

E

V

T

L

L

E

H

D

H

A

L

D

K

E

V

M

A

T

E

E

G

A

F

G

G

L

L

G

K

F

-

T

A

R

K

P

P

Q

E

L

L

D

D

L

L

D

K

K

K

L

L

R

G

W

W

K

R

S

D

G

Q

I

V

V

V

S

K

K

K

L

L

T

T

G

G

L

V

A

G

A

T

L

L

A

L

R

K

Q

G

R

N

K

G

V

V

I

E

V

L

T

R

G

G

F
|
F

G
x
A

R

R

F

L

V

V

S

G

P

P

N

K

E

E

I

V

E

E

V

V

T

G

H

G

E

E

G

-

A

-

T

-

K

-

R

R

V

Y

A

G

F

A

T

K

Q

S

A

L

I

I

I

L

A
|
A

A
x
T

G
|
G

S

S

E

E

P

P

A

L

R

E

L

L

P

K

I

G

A

F

P

P

K

F

H

G

D

E

S

D

R

-

I

V

L

W

D

D

S
|
S

T

T

G

R

A

A

L

L

D

K

L

V

Q

E

D

E

-

G

V

L

P

P

P

K

R

R

L

L

V

V

V

I

G

G

I
x
A

I

V

G

G

L

L

E
|
E

M

L

A

G

T

Q

V

V

Y

Y

H

R

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

L

V

I

E

E

A

Y

M

M

D

P

Q

E

L

I

I

L

S

P

G

Q

A

G

D

D

R

P

D

E

I

T

V

A

A

A

P

L

L

L

M

R

K

R

R

A

I

L

S

E

T

K

R

E

Y

G

D

I

A

R

V

V

H

R

L

T

G

K

T

T

K

K

V

A

R

V

N

G

I

Y

A

E

S

K

A

K

A

D

E

G

L

L

T

H

V

V

T

R

F

L

E

E

G

P

A

A

N

E

A

G

P

G

E

E

E

G

Q

E

S

E

F

V

D

V

K

V

I

D

L

K

V

V

A

L

V

V

-

A

-

V

G

G

R

R

V

K

P

P

N
x
R

S

T

G

E

T

G

L

L

G

G

L

L

A

E

A

K

A

A

G

G

V

V

A

K

V

V

D

D

Q

E

R

R

G

G

F

F

I

I

R

R

V

V

D

N

K

A

Q

R

M

M

R

E

T

T

N

S

V

V

P

P

H

G

I

V

F

Y

A

A

I

I

G

G

D
|
D

V

A

G

R

Q

P

P

P

M
x
L

L
|
L

A
|
A

H
|
H

K

K

A

A

V

M

H

R

E

E

G

G

K

L

V

I

A

A

E

E

V

N

A

A

G

G

L

K

K

D

A

S

A

A

F

F

D

D

A

Y

R

Q

V

V

I

-

P

P

S

S

V

V

A

V

Y
|
Y

T

T

D

S

P

P

E

E

V

W

A

A

W

G

V

V

G

G

L

L

T

T

E

E

A

E

E

E

A

A

N

K

A

R

N

A

G

G

V

Y

A

K

Y

V

G

K

K

V

G

G

S

K

F

F

P

P

W

L

E

A

A

A

S

S

G

G

R

R

A

A

L

L

S

T

Q

L

G

G

R

G

S

A

E

E

G

G

L

M

T

V

K

K

V

V

L

V

F

G

D

D

P

E

E

E

T

T

H

D

R

L

T

L

L

L

G

G

C

V

G

F

I

I

V

V

G

G

A

P

H

Q

A

A

G

G

E

E

L

L

I

I

G

A

E

E

M

A

V

A

L

L

A

A

I

L

E

E

M

M

G

G

A

A

D

T

A

L

A

T

D

D

I

L

A

A

G

L

T

T

I

V

H
|
H

P

P

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

S

I

L

A

M

Q

E

S

A

A

A

E

E

M

A

F

F

active site: V37 (≠ L43), C41 (= C47), C46 (= C52), T49 (≠ S55), A176 (≠ I187), E180 (= E191), H435 (= H445), H437 (= H447), E442 (= E452)
binding flavin-adenine dinucleotide: I9 (≠ L14), G10 (= G15), G12 (= G17), P13 (= P18), G14 (= G19), E33 (= E38), A34 (≠ R39), G39 (= G45), V40 (= V46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), F111 (= F118), A112 (≠ G119), A135 (= A146), T136 (≠ A147), G137 (= G148), S155 (= S167), R269 (≠ N278), D306 (= D315), L312 (≠ M321), L313 (= L322), A314 (= A323), H315 (= H324), Y344 (= Y354)

Sites not aligning to the query:

active site: 460

6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
42% identity, 95% coverage: 10:463/476 of query aligns to 6:467/475 of 6awaA

query
sites
6awaA

D

D

I

V

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

C

V

I

E
|
E

R
x
K

G

Y

P

T

-

D

-

A

-

E

-

G

-

K

-

L

A

A

L
|
L

G

G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

A

S

K

W

V

K

L

Y

E

K

D

E

A

A

D

K

E

E

M

S

T

F

E

N

A

V

-

H

G

G

L

I

G

S

F

T

T

G

R

E

P

V

Q

K

L

M

D

D

L

V

D

A

K

A

L

M

R

V

R

G

W

R

K

K

S

A

G

G

I

I

V

V

S

K

K

N

L

L

T

T

G

G

L

V

A

A

A

T

L

L

A

F

R

K

Q

A

R

N

K

G

V

V

I

T

V

S

V

I

T

Q

G

G

F
x
H

G
|
G

R

K

F

L

V

L

S

A

P

G

N

K

E

K

I

V

E

E

V

V

T

T

H

K

-

A

E

D

G

G

A

T

T

T

K

E

R

V

V

I

A

E

F

A

T

E

Q

N

A

V

I

I

I

L

A

A

A
x
S

G
|
G

S

S

E

R

P

P

A

I

R

D

L

I

P

P

I

P

A

A

P

P

K

V

H

D

D

Q

S

N

R

V

I

I

L

V

D

D

S

S

T

T

G

G

A

A

L

L

D

E

L

F

Q

Q

D

A

V

V

P

P

P

K

R

R

L

L

L

G

V

V

V
x
I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E
|
E

A
|
A

M
x
L

D

D

Q

T

L

F

I

L

S

M

G

A

A

A

D

D

R

T

D

A

I

V

V

S

A

K

P

E

L

A

M

Q

K

K

R

T

I

L

S

T

T

K

R

Q

Y

G

D

L

A

D

V

I

H

K

L

L

G

G

T

A

K

R

V
|
V

R

T

N

G

I

S

A

K

S

V

A

N

A

G

A

N

E

E

L

V

T

E

V

V

T

T

F

Y

E

T

G

N

A

A

N

E

A

G

P

E

E

Q

E

K

Q

I

S

T

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V
|
V

G

G

R
|
R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

S

A

D

A

S

G

G

V

V

A

T

V

I

D

D

Q

E

R

R

G

G

F

Y

I

I

R

F

V

V

D

D

K

D

Q

Y

M

C

R

A

T

T

N

S

V

V

P

P

H

G

I

V

F

Y

A

A

I

I

G
|
G

D
|
D

V

V

G

R

Q

G

P

M

M
|
M

L
|
L

A
|
A

H

H

K

K

A

A

V

S

H

E

E

E

G

G

K

I

V

M

A

V

E

E

V

R

A

I

A

K

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

S

V

V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

A

A

L

N

K

A

A

N

E

G

G

V

V

A

E

Y

V

G

N

K

V

G

G

S

T

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

G

G

G

L

F

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

A

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

P

S

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

A

I

L

A

H

Q

E

S

A

A

A

E

L

M

A

F

V

E

N

G

G

active site: L45 (= L43), C49 (= C47), C54 (= C52), S57 (= S55), V191 (≠ I187), E195 (= E191), F449 (≠ H445), H451 (= H447), E456 (= E452)
binding adenosine monophosphate: I187 (≠ V183), E211 (= E207), A212 (= A208), L213 (≠ M209), V245 (= V241), V277 (= V273)
binding flavin-adenine dinucleotide: I10 (≠ L14), G13 (= G17), P14 (= P18), G15 (= G19), E34 (= E38), K35 (≠ R39), T48 (≠ V46), C49 (= C47), G53 (= G51), C54 (= C52), K58 (= K56), H121 (≠ F118), G122 (= G119), S151 (≠ A147), G152 (= G148), I192 (= I188), R279 (= R275), G318 (= G314), D319 (= D315), M325 (= M321), L326 (= L322), A327 (= A323), Y358 (= Y354)

Sites not aligning to the query:

active site: 474, 475

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
42% identity, 94% coverage: 10:458/476 of query aligns to 2:456/465 of 3urhB

query
sites
3urhB

D

D

I

L

V

I

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

C

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

M

K

K

V

V

V

A

V

V

E
|
E

R
x
K

G

R

P

S

A

T

L
x
Y

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

H

A

A

A

S

K

E

V

M

L

F

E

H

D

Q

A

A

D

Q

E

H

M

G

T

L

E

E

A

A

-

L

G

G

L

V

G

E

F

V

T

A

R

N

P

P

Q

K

L

L

D

N

L

L

D

Q

K

K

L

M

R

M

R

A

W

H

K

K

S

D

G

A

I

T

V

V

S

K

K

S

L

N

T

V

G

D

G

G

L

V

A

S

A

F

L

L

A

F

R

K

Q

K

R

N

K

K

V

I

I

D

V

G

V

F

T

Q

G

G

F
x
T

G
|
G

R

K

F

V

V

L

S

G

P

Q

N

G

E

K

I

V

E

S

V

V

T

T

H

N

E

E

-

K

G

G

A

E

T

E

K

Q

R

V

V

L

A

E

F

A

T

K

Q

N

A

V

I

V

I

I

A
|
A

A
x
T

G
|
G

S

S

E

D

P

V

A

A

R

G

L

I

P

P

-

G

I

V

A

E

P

V

K

A

H

F

D

D

S

E

R

K

-

T

I

I

L

V

D

S

S

S

T

T

G

G

A

A

L

L

D

A

L

L

Q

E

D

K

V

V

P

P

P

A

R

S

L

M

L

I

V

V

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

K

V

V

T

T

V

V

E

E

A

F

M

L

D

D

Q

T

L

I

I

L

S

G

G

G

A

M

D

D

R

G

D

E

I

V

V

A

A

K

P

Q

L

L

M

Q

K

R

R

M

I

L

S

T

T

K

R

Q

Y

G

D

I

A

D

V

F

H

K

L

L

G

G

T

A

K

K

V

V

R

T

N

G

I

A

A

V

S

K

A

S

A

G

A

D

E

G

L

A

T

K

V

V

T

T

F

F

E

E

G

P

A

V

N

K

A

G

P

G

E

E

E

A

Q

T

S

T

F

L

D

D

K

A

-

E

-

V

I

V

L

L

V

I

A

A

V

T

G

G

R
|
R

V

K

P

P

N

S

S

T

G

D

T

G

L

L

G

G

L

L

A

A

A

K

A

A

G

G

V

V

A

V

V

L

D

D

Q

S

R

R

G

G

F

R

I

V

R

E

V

I

D

D

K

R

Q

H

M

F

R

Q

T

T

N

S

V

I

P

A

H

G

I

V

F

Y

A

A

I

I

G
|
G

D
|
D

V

V

G

R

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

D

E

E

G

G

K

V

V

A

A

V

A

A

E

E

V

I

A

I

A

A

G

G

L

Q

K

A

A

G

A

H

F

V

D

N

A

Y

R

D

V

V

I

I

P

P

S

G

V

V

A

V

Y

Y

T

T

D

Q

P

P

E

E

V

V

A

A

W

S

V

V

G

G

L

K

T

T

E

E

A

E

E

E

A

L

N

K

A

A

N

A

G

G

V

V

A

A

Y

Y

G

K

K

I

G

G

S

K

F

F

P

P

W

F

E

T

A

A

S

N

G

G

R

R

A

A

L

R

S

A

Q

M

G

L

R

Q

S

T

E

D

G

G

L

F

T

V

K

K

V

I

L

L

F

A

D

D

P

K

E

E

T

T

H

D

R

R

T

V

L

L

G

G

C

G

G

H

I

I

V

I

G

G

A

F

H

G

A

A

G

G

E

E

L

M

I

I

G

H

E

E

M

I

V

A

L

V

A

L

I

M

E

E

M

F

G

G

A

G

D

S

A

S

A

E

D

D

I

L

A

G

G

R

T

T

I

C

H
|
H

P

A

H
|
H

P

P

T

T

L

M

S

S

E
|
E

T

A

I

V

A

K

Q

E

S

A

A

A

active site: Y35 (≠ L43), C39 (= C47), C44 (= C52), S47 (= S55), V183 (≠ I187), E187 (= E191), H443 (= H445), H445 (= H447), E450 (= E452)
binding flavin-adenine dinucleotide: I6 (≠ L14), G7 (= G15), G9 (= G17), P10 (= P18), G11 (= G19), E30 (= E38), K31 (≠ R39), G37 (= G45), T38 (≠ V46), C39 (= C47), G43 (= G51), C44 (= C52), K48 (= K56), T111 (≠ F118), G112 (= G119), A140 (= A146), T141 (≠ A147), G142 (= G148), I184 (= I188), R273 (= R275), G312 (= G314), D313 (= D315), M319 (= M321), L320 (= L322), A321 (= A323), H322 (= H324)

6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
42% identity, 94% coverage: 10:458/476 of query aligns to 8:459/470 of 6uziC

query
sites
6uziC

D

D

I

V

V

A

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

R

A

C

A

A

D

Q

L

L

G

G

K

F

K

K

V

T

V

V

V

I

E
|
E

R
x
K

G

Y

P

S

A

T

L

L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

A

S

K

E

V

H

L

F

E

E

D

N

A

A

D

K

E

H

-

T

M

F

T

A

E

T

A

H

G

G

L

I

G

L

F

I

T

D

R

E

P

P

Q

K

L

V

D

D

L

I

D

A

K

Q

L

M

R

I

R

S

W

R

K

K

S

N

G

D

I

V

V

V

S

D

K

Q

L

T

T

T

G

K

G

G

L

I

A

N

A

F

L

L

A

M

R

D

Q

K

R

N

K

K

V

I

I

T

V

V

V

L

T

Q

G

G

F
x
V

G
|
G

R

S

F

F

V

E

S

S

P

A

N

T

E

Q

I

I

E

K

V

V

T

T

H

K

-

A

E

D

G

G

A

S

T

S

K

E

R

V

V

I

A

E

F

A

T

K

Q

N

A

T

I

I

A

A

A
x
T

G
|
G

S

S

E

K

P

P

A

S

R

S

L

L

P

P

I

F

A

I

P

T

K

L

H

D

D

K

S

E

R

R

I

V

L

I

D

T

S

S

T

T

G

E

A

A

L

L

D

N

L

L

Q

K

D

E

V

V

P

P

P

K

R

H

L

L

L

I

V

V

V

I

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

Y

H

L

A

R

L

L

G

G

S

S

R

D

V

V

T

T

V

V

E

E

A

Y

M

L

D

D

Q

K

L

I

I

I

S

P

G

G

A

M

D

D

R

G

D

T

I

L

V

S

A

K

P

E

L

L

M

Q

K

K

R

T

I

L

S

K

T

K

R

Q

Y

G

D

M

A

K

V

F

H

M

L

L

G

S

T

T

K

A

V

V

R

S

N

G

I

V

A

E

S

R

A

N

A

G

A

D

E

T

L

V

T

K

V

V

T

T

F

A

E

K

G

D

A

K

N

K

A

G

P

E

E

D

E

V

Q

V

-

V

S

E

F

G

D

D

K

Y

I

C

L

L

V

V

A

S

V

V

G

G

R
|
R

V

R

P

P

N

Y

S

T

G

D

T

G

L

L

G

G

L

L

A

E

A

K

A

A

G

G

V

V

A

E

V

L

D

D

Q

E

R

R

G

G

F

R

I

V

R

K

V

T

D

N

K

D

Q

H

M

L

R

Q

T

T

N

N

V

V

P

P

H

N

I

I

F

Y

A

A

I

I

G

G

D
|
D

V

V

G

K

Q

G

P

A

M
|
M

L
|
L

A
|
A

H

H

K

K

A

A

V

E

H

E

E

E

G

G

K

V

V

F

A

V

A

A

E

E

V

T

A

L

A

A

G

G

L

E

K

K

A

P

A

H

F

V

D

N

A

Y

R

N

V

L

I

I

P

P

S

G

V

V

A

V

Y

Y

T

T

D

W

P

P

E

E

V

V

A

A

W

G

V

V

G

G

L

K

T

T

E

E

A

E

E

Q

A

L

N

K

A

E

N

A

G

G

V

V

A

A

Y

Y

G

K

K

T

G

G

S

S

F

F

P

P

W

M

E

R

A

A

S

L

G

G

R

R

A

S

L

R

S

A

Q

S

G

M

R

D

S

T

E

D

G

G

L

V

T

I

K

K

V

I

L

L

F

A

D

D

P

E

E

K

T

T

H

D

R

E

T

I

L

L

G

G

C

V

G

H

I

M

V

I

G

G

A

A

H

R

A

A

G

A

E

D

L

M

I

I

G

A

E

E

M

A

V

V

L

V

A

A

I

M

E

E

M

F

G

R

A

A

D

S

A

A

A

E

D

D

I

I

A

A

G

R

T

I

I

S

H

H

P

A

H
|
H

P

P

T

T

L

Y

S

T

E
|
E

T

A

I

I

A

K

Q

E

S

A

A

A

active site: C45 (= C47), C50 (= C52), S53 (= S55), V187 (≠ I187), E191 (= E191), H448 (= H447), E453 (= E452)
binding flavin-adenine dinucleotide: I12 (≠ L14), G13 (= G15), G15 (= G17), P16 (= P18), G17 (= G19), E36 (= E38), K37 (≠ R39), G43 (= G45), T44 (≠ V46), C45 (= C47), G49 (= G51), C50 (= C52), S53 (= S55), K54 (= K56), V117 (≠ F118), G118 (= G119), T147 (≠ A147), G148 (= G148), I188 (= I188), R276 (= R275), D316 (= D315), M322 (= M321), L323 (= L322), A324 (= A323)
binding zinc ion: H448 (= H447), E453 (= E452)

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
42% identity, 94% coverage: 10:458/476 of query aligns to 6:462/477 of P18925

query
sites
P18925

D

D

I

V

V

I

V

V

L

I

G

G

A

A

G

G

P

P

G

G

Y

Y

T

V

A

A

F

I

R

K

A

S

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

L

V

I

E
|
E

R
x
K

-
x
Y

-
x
K

-
x
G

-
x
K

-
x
E

-
x
G

G
x
K

P
x
T

A
|
A

L
|
L

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

H

D

A

S

K

Y

V

K

L

F

E

H

D

E

A

A

D

H

E

E

M

S

T

F

E

K

A

L

-

H

G

G

L

I

G

S

F

T

T

G

R

E

P

V

Q

A

L

I

D

D

L

V

D

P

K

T

L

M

R

I

R

A

W

R

K

K

S

D

G

Q

I

I

V

V

S

R

K

N

L

L

T

T

G

G

L

V

A

A

A

S

L

L

A

I

R

K

Q

A

R

N

K

G

V

V

I

T

V

L

V

F

T

E

G

G

F

H

G

G

R

K

F

L

V

L

S

A

P

G

N

K

E

K

I

V

E

E

V

V

T

T

H

A

E

A

G

D

A

G

T

S

K

S

R

Q

V

V

A

L

F

D

T

T

Q

E

A

N

I

V

I

I

-

L

A

A

A

S

G

G

S

S

E

K

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

V

H

D

D

Q

S

D

R

V

I

I

L

V

D

D

S

S

T

T

G

G

A

A

L

L

D

D

L

F

Q

Q

D

N

V

V

P

P

P

G

R

K

L

L

L

G

V

V

V

I

G

G

G

A

G

G

I

V

I

I

G

G

L

L

E

E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E

E

A

A

M

M

D

D

Q

K

L

F

I

L

S

P

G

A

A

V

D

D

R

E

D

Q

I

V

V

A

A

K

P

E

L

A

M

Q

K

K

R

I

I

L

S

T

T

K

R

Q

Y

G

D

L

A

K

V

I

H

L

L

L

G

G

T

A

K

R

V

V

R

T

N

G

I

T

A

E

S

V

A

K

A

N

A

K

E

Q

L

V

T

T

V

V

T

K

F

F

E

V

G

D

A

A

N

E

A

G

P

E

E

K

E

S

Q

Q

S

A

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V

V

G

G

R

R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

Y

M

C

R

A

T

T

N

S

V

V

P

P

H

G

I

V

F

Y

A

A

I

I

G

G

D
|
D

V

V

G

R

Q

G

P

A

M

M

L

L

A
|
A

H

H

K

K

A

A

V

S

H

E

E

E

G

G

K

V

V

V

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

A

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

G

V

V

G

G

L

K

T

T

E

E

A

Q

E

A

A

L

N

K

A

A

N

E

G

G

V

V

A

A

Y

I

G

N

K

V

G

G

S

V

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

A

G

G

L

F

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

A

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H

F

P

A

H

H

P

P

T

A

L

L

S

S

E

E

T

A

I

L

A

H

Q

E

S

A

A

A

34:49 (vs. 38:47, 38% identical) binding FAD
C49 (= C47) modified: Disulfide link with 54, Redox-active
C54 (= C52) modified: Disulfide link with 49, Redox-active
K58 (= K56) binding FAD
D319 (= D315) binding FAD
A327 (= A323) binding FAD

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
42% identity, 94% coverage: 10:458/476 of query aligns to 5:461/472 of 3ladA

query
sites
3ladA

D

D

I

V

V

I

V

V

L
x
I

G
|
G

A

A

G
|
G

P
|
P

G

G

Y

Y

T

V

A

A

F

I

R

K

A

S

A

A

D

Q

L

L

G

G

K

L

K

K

V

T

V

A

V

L

V

I

E
|
E

R
x
K

-

Y

-

K

-

G

-

K

-

E

-

G

G

K

P

T

A

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K

K

A

A

L

L

H

D

A

S

K

Y

V

K

L

F

E

H

D

E

A

A

D

H

E

E

M

S

T

F

E

K

A

L

-

H

G

G

L

I

G

S

F

T

T

G

R

E

P

V

Q

A

L

I

D

D

L

V

D

P

K

T

L

M

R

I

R

A

W

R

K

K

S

D

G

Q

I

I

V

V

S

R

K

N

L

L

T

T

G

G

L

V

A

A

A

S

L

L

A

I

R

K

Q

A

R

N

K

G

V

V

I

T

V

L

V

F

T

E

G

G

F
x
H

G
|
G

R

K

F

L

V

L

S

A

P

G

N

K

E

K

I

V

E

E

V

V

T

T

H

A

E

A

G

D

A

G

T

S

K

S

R

Q

V

V

A

L

F

D

T

T

Q

E

A

N

I

V

I

I

-

L

A
|
A

A
x
S

G
|
G

S

S

E

K

P

P

A

V

R

E

L

I

P

P

I

P

A

A

P

P

K

V

H

D

D

Q

S

D

R

V

I

I

L

V

D

D

S

S

T

T

G

G

A

A

L

L

D

D

L

F

Q

Q

D

N

V

V

P

P

P

G

R

K

L

L

L

G

V

V

V

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

A

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

V

V

L

E

E

A

A

M

M

D

D

Q

K

L

F

I

L

S

P

G

A

A

V

D

D

R

E

D

Q

I

V

V

A

A

K

P

E

L

A

M

Q

K

K

R

I

I

L

S

T

T

K

R

Q

Y

G

D

L

A

K

V

I

H

L

L

L

G

G

T

A

K

R

V

V

R

T

N

G

I

T

A

E

S

V

A

K

A

N

A

K

E

Q

L

V

T

T

V

V

T

K

F

F

E

V

G

D

A

A

N

E

A

G

P

E

E

K

E

S

Q

Q

S

A

F

F

D

D

K

K

I

L

L

I

V

V

A

A

V

V

G

G

R
|
R

V

R

P

P

N

V

S

T

G

T

T

D

L

L

G

L

L

A

A

A

A

D

A

S

G

G

V

V

A

T

V

L

D

D

Q

E

R

R

G

G

F

F

I

I

R

Y

V

V

D

D

K

D

Q

Y

M

C

R

A

T

T

N

S

V

V

P

P

H

G

I

V

F

Y

A

A

I

I

G

G

D
|
D

V

V

G

R

Q

G

P

A

M

M

L
|
L

A
|
A

H

H

K

K

A

A

V

S

H

E

E

E

G

G

K

V

V

V

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

H

K

K

A

A

A

Q

F

M

D

N

A

Y

R

D

V

L

I

I

P

P

S

A

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

G

V

V

G

G

L

K

T

T

E

E

A

Q

E

A

A

L

N

K

A

A

N

E

G

G

V

V

A

A

Y

I

G

N

K

V

G

G

S

V

F

F

P

P

W

F

E

A

A

A

S

S

G

G

R

R

A

A

L

M

S

A

Q

A

G

N

R

D

S

T

E

A

G

G

L

F

T

V

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

R

T

V

L

L

G

G

C

V

G

H

I

V

V

I

G

G

A

P

H

S

A

A

G

A

E

E

L

L

I

V

G

Q

E

Q

M

G

V

A

L

I

A

A

I

M

E

E

M

F

G

G

A

T

D

S

A

A

A

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

P

A

H
|
H

P

P

T

A

L

L

S

S

E
|
E

T

A

I

L

A

H

Q

E

S

A

A

A

active site: L44 (= L43), C48 (= C47), C53 (= C52), S56 (= S55), V190 (≠ I187), E194 (= E191), F448 (≠ H445), H450 (= H447), E455 (= E452)
binding flavin-adenine dinucleotide: I9 (≠ L14), G10 (= G15), G12 (= G17), P13 (= P18), E33 (= E38), K34 (≠ R39), G46 (= G45), T47 (≠ V46), C48 (= C47), G52 (= G51), C53 (= C52), H120 (≠ F118), G121 (= G119), A149 (= A146), S150 (≠ A147), G151 (= G148), I191 (= I188), R278 (= R275), D318 (= D315), L325 (= L322), A326 (= A323)

6hg8B Crystal structure of the r460g disease-causing mutant of the human dihydrolipoamide dehydrogenase.
40% identity, 95% coverage: 7:457/476 of query aligns to 13:470/482 of 6hg8B

query
sites
6hg8B

I

I

E

D

A

A

D

D

I

V

V

T

V

V

L
x
I

G

G

A

S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

F

K

K

V

T

V

V

V

C

V

I

E
|
E

R
x
K

G
x
N

P

E

A

T

L

L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

N

A

N

A

S

K

H

V

Y

L

Y

E

H

D

M

A

A

-

H

-

G

D

K

E

D

M

F

T

A

E

S

A

R

G

G

L

I

G

E

F

M

T

S

R

E

P

V

Q

R

L

L

D

N

L

L

D

D

K

K

L

M

R

M

R

E

W

Q

K

K

S

S

G

T

I

A

V

V

S

K

K

A

L

L

T

T

G

G

L

I

A

A

A

H

L

L

A

F

R

K

Q

Q

R

N

K

K

V

V

I

V

V

H

V

V

T

N

G

G

F
x
Y

G
|
G

R

K

F

I

V

T

S

G

P

K

N

N

E

Q

I

V

E

T

V

A

T

T

H

K

-

A

E

D

G

G

A

G

T

T

K

Q

R

V

V

I

A

D

F

T

T

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S

S

E

E

P

V

A

T

R

P

L

F

P

P

I

G

A

I

P

T

K

I

H

D

D

E

S

D

R

T

I

I

L

V

D

S

S

S

T

T

G

G

A

A

L

L

D

S

L

L

Q

K

D

K

V

V

P

P

P

E

R

K

L

M

L

V

V

V

V

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

V

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

Q

A

R

L

L

G

G

S

A

R

D

V

V

T

T

V

A

V

V

E

E

A

F

M

L

D

G

Q

H

L

V

I

G

S

G

-

V

G

G

A

I

D

D

R

M

D

E

I

I

V

S

A

K

P

N

L

F

M

Q

K

R

R

I

I

L

S

Q

T

K

R

Q

Y

G

D

F

A

K

V

F

H

K

L

L

G

N

T

T

K

K

V

V

R

T

N

G

I

A

A

T

S

K

A

K

A

S

-

D

A

G

E

K

L

I

T

D

V

V

T

S

F

I

E

E

G

A

A

A

N

S

A

G

P

G

E

K

E

A

Q

E

-

V

-

I

S

T

F

C

D

D

K

V

I

L

L

L

V

V

A

C

V

I

G

G

R
|
R

V

R

P

P

N
x
F

S

T

G

K

T

N

L

L

G

G

L

L

A

E

A

L

G

G

V

I

A

E

V

L

D

D

Q

P

R

R

G

G

F

R

I

I

R

P

V

V

D

N

K

T

Q

R

M

F

R

Q

T

T

N

K

V

I

P

P

H

N

I

I

F

Y

A

A

I

I

G
|
G

D
|
D

V

V

G

A

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

D

E

E

G

G

K

I

V

I

A

C

A

V

E

E

V

G

A

M

A

A

G

G

L

G

K

A

A

V

A

H

F

I

D

D

A

Y

R

N

V

C

I

V

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

A

E

E

Q

A

L

N

K

A

E

N

E

G

G

V

I

A

E

Y

Y

G

K

K

V

G

G

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

S

R

R

A

A

L

K

S

T

Q

N

G

A

R

D

S

T

E

D

G

G

L

M

T

V

K

K

V

I

L

L

F

G

D

Q

P

K

E

S

T

T

H

D

R

R

T

V

L

L

G

G

C

A

G

H

I

I

V

L

G

G

A

P

H

G

A

A

G

G

E

E

L

M

I

V

G

N

E

E

M

A

V

A

L

L

A

A

I

L

E

E

M

Y

G

G

A

A

D

S

A

C

A

E

D

D

I

I

A

A

G

R

T

V

I

C

H

H

P

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

A

I

F

A

G

Q

E

S

A

active site: C53 (= C47), C58 (= C52), S61 (= S55), V196 (≠ I187), E200 (= E191), H460 (= H447), E465 (= E452)
binding flavin-adenine dinucleotide: I20 (≠ L14), G23 (= G17), P24 (= P18), G25 (= G19), E44 (= E38), K45 (≠ R39), N46 (≠ G40), G51 (= G45), T52 (≠ V46), C53 (= C47), G57 (= G51), C58 (= C52), K62 (= K56), Y126 (≠ F118), G127 (= G119), T156 (≠ A147), G157 (= G148), I197 (= I188), R288 (= R275), F291 (≠ N278), G327 (= G314), D328 (= D315), M334 (= M321), L335 (= L322), A336 (= A323), H337 (= H324)

1zmdA Crystal structure of human dihydrolipoamide dehydrogenase complexed to nadh (see paper)
40% identity, 95% coverage: 7:457/476 of query aligns to 3:460/472 of 1zmdA

query
sites
1zmdA

I

I

E

D

A

A

D

D

I

V

V

T

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

F

K

K

V

T

V

V

V

C

V

I

E
|
E

R
x
K

G
x
N

P

E

A

T

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

N

A

N

A

S

K

H

V

Y

L

Y

E

H

D

M

A

A

D

H

-

G

-

T

E

D

M

F

T

A

E

S

A

R

G

G

L

I

G

E

F

M

T

S

R

E

P

V

Q

R

L

L

D

N

L

L

D

D

K

K

L

M

R

M

R

E

W

Q

K

K

S

S

G

T

I

A

V

V

S

K

K

A

L

L

T

T

G

G

L

I

A

A

A

H

L

L

A

F

R

K

Q

Q

R

N

K

K

V

V

I

V

V

H

V

V

T

N

G

G

F
x
Y

G
|
G

R

K

F

I

V

T

S

G

P

K

N

N

E

Q

I

V

E

T

V

A

T

T

H

K

-

A

E

D

G

G

A

G

T

T

K

Q

R

V

V

I

A

D

F

T

T

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S

S

E

E

P

V

A

T

R

P

L

F

P

P

I

G

A

I

P

T

K

I

H

D

D

E

S

D

R

T

I

I

L

V

D

S

S
|
S

T

T

G

G

A

A

L

L

D

S

L

L

Q

K

D

K

V

V

P

P

P

E

R

K

L

M

L

V

V

V

V
x
I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

V

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

Q

A

R

L

L

G

G

S

A

R

D

V

V

T

T

V

A

V

V

E
|
E

A
x
F

M
x
L

D

G

Q

H

L

V

I

G

S

G

-

V

G

G

A

I

D

D

R

M

D

E

I

I

V

S

A

K

P

N

L

F

M

Q

K

R

R

I

I

L

S

Q

T

K

R

Q

Y

G

D

F

A

K

V

F

H

K

L

L

G

N

T

T

K

K

V

V

R

T

N

G

I

A

A

T

S

K

A

K

A

S

-

D

A

G

E

K

L

I

T

D

V

V

T

S

F

I

E

E

G

A

A

A

N

S

A

G

P

G

E

K

E

A

Q

E

-

V

-

I

S

T

F

C

D

D

K

V

I

L

L

L

V

V

A

C

V
x
I

G
|
G

R
|
R

V

R

P

P

N
x
F

S

T

G

K

T

N

L

L

G

G

L

L

A

E

A

L

G

G

V

I

A

E

V

L

D

D

Q

P

R

R

G

G

F

R

I

I

R

P

V

V

D

N

K

T

Q

R

M

F

R

Q

T

T

N

K

V

I

P

P

H

N

I

I

F

Y

A

A

I

I

G
|
G

D
|
D

V

V

G

A

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

D

E

E

G

G

K

I

V

I

A

C

A

V

E

E

V

G

A

M

A

A

G

G

L

G

K

A

A

V

A

H

F

I

D

D

A

Y

R

N

V

C

I

V

P

P

S

S

V
|
V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

A

E

E

Q

A

L

N

K

A

E

N

E

G

G

V

I

A

E

Y

Y

G

K

K

V

G

G

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

S

R

R

A

A

L

K

S

T

Q

N

G

A

R

D

S

T

E

D

G

G

L

M

T

V

K

K

V

I

L

L

F

G

D

Q

P

K

E

S

T

T

H

D

R

R

T

V

L

L

G

G

C

A

G

H

I

I

V

L

G

G

A

P

H

G

A

A

G

G

E

E

L

M

I

V

G

N

E

E

M

A

V

A

L

L

A

A

I

L

E

E

M

Y

G

G

A

A

D

S

A

C

A

E

D

D

I

I

A

A

G

R

T

V

I

C

H
|
H

P

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

A

I

F

A

R

Q

E

S

A

active site: L39 (= L43), C43 (= C47), C48 (= C52), S51 (= S55), V186 (≠ I187), E190 (= E191), H448 (= H445), H450 (= H447), E455 (= E452)
binding flavin-adenine dinucleotide: I10 (≠ L14), G11 (= G15), G13 (= G17), P14 (= P18), G15 (= G19), E34 (= E38), K35 (≠ R39), N36 (≠ G40), G41 (= G45), T42 (≠ V46), C43 (= C47), G47 (= G51), C48 (= C52), K52 (= K56), Y116 (≠ F118), G117 (= G119), T146 (≠ A147), G147 (= G148), S166 (= S167), R278 (= R275), F281 (≠ N278), G317 (= G314), D318 (= D315), M324 (= M321), L325 (= L322), A326 (= A323), H327 (= H324)
binding 1,4-dihydronicotinamide adenine dinucleotide: I182 (≠ V183), G183 (= G184), G185 (= G186), V186 (≠ I187), I187 (= I188), E190 (= E191), E206 (= E207), F207 (≠ A208), L208 (≠ M209), I276 (≠ V273), G277 (= G274), R278 (= R275), M324 (= M321), L325 (= L322), V355 (= V352), Y357 (= Y354)

1zmcA Crystal structure of human dihydrolipoamide dehydrogenase complexed to NAD+ (see paper)
40% identity, 95% coverage: 7:457/476 of query aligns to 3:460/472 of 1zmcA

query
sites
1zmcA

I

I

E

D

A

A

D

D

I

V

V

T

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

F

K

K

V

T

V

V

V

C

V

I

E
|
E

R
x
K

G
x
N

P

E

A

T

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

N

A

N

A

S

K

H

V

Y

L

Y

E

H

D

M

A

A

D

H

-

G

-

T

E

D

M

F

T

A

E

S

A

R

G

G

L

I

G

E

F

M

T

S

R

E

P

V

Q

R

L

L

D

N

L

L

D

D

K

K

L

M

R

M

R

E

W

Q

K

K

S

S

G

T

I

A

V

V

S

K

K

A

L

L

T

T

G

G

L

I

A

A

A

H

L

L

A

F

R

K

Q

Q

R

N

K

K

V

V

I

V

V

H

V

V

T

N

G

G

F
x
Y

G
|
G

R

K

F

I

V

T

S

G

P

K

N

N

E

Q

I

V

E

T

V

A

T

T

H

K

-

A

E

D

G

G

A

G

T

T

K

Q

R

V

V

I

A

D

F

T

T

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S

S

E

E

P

V

A

T

R

P

L

F

P

P

I

G

A

I

P

T

K

I

H

D

D

E

S

D

R

T

I

I

L

V

D

S

S
|
S

T

T

G

G

A

A

L

L

D

S

L

L

Q

K

D

K

V

V

P

P

P

E

R

K

L

M

L

V

V

V

V

I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

V

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

Q

A

R

L

L

G

G

S

A

R

D

V

V

T

T

V

A

V
|
V

E
|
E

A
x
F

M
x
L

D

G

Q

H

L

V

I

G

S

G

-

V

G

G

A

I

D

D

R

M

D

E

I

I

V

S

A

K

P

N

L

F

M

Q

K

R

R

I

I

L

S

Q

T

K

R

Q

Y

G

D

F

A

K

V

F

H

K

L

L

G

N

T

T

K
|
K

V
|
V

R

T

N

G

I

A

A

T

S

K

A

K

A

S

-

D

A

G

E

K

L

I

T

D

V

V

T

S

F

I

E

E

G

A

A

A

N

S

A

G

P

G

E

K

E

A

Q

E

-

V

-

I

S

T

F

C

D

D

K

V

I

L

L

L

V

V

A

C

V
x
I

G
|
G

R
|
R

V

R

P

P

N
x
F

S

T

G

K

T

N

L

L

G

G

L

L

A

E

A

L

G

G

V

I

A

E

V

L

D

D

Q

P

R
|
R

G

G

F

R

I

I

R

P

V

V

D

N

K

T

Q

R

M

F

R

Q

T

T

N

K

V

I

P

P

H

N

I

I

F

Y

A

A

I

I

G
|
G

D
|
D

V

V

G

A

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

D

E

E

G

G

K

I

V

I

A

C

A

V

E

E

V

G

A

M

A

A

G

G

L

G

K

A

A

V

A

H

F

I

D

D

A

Y

R

N

V

C

I

V

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

A

E

E

Q

A

L

N

K

A

E

N

E

G

G

V

I

A

E

Y

Y

G

K

K

V

G

G

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

S

R

R

A

A

L

K

S

T

Q

N

G

A

R

D

S

T

E

D

G

G

L

M

T

V

K

K

V

I

L

L

F

G

D

Q

P

K

E

S

T

T

H

D

R

R

T

V

L

L

G

G

C

A

G

H

I

I

V

L

G

G

A

P

H

G

A

A

G

G

E

E

L

M

I

V

G

N

E

E

M

A

V

A

L

L

A

A

I

L

E

E

M

Y

G

G

A

A

D

S

A

C

A

E

D

D

I

I

A

A

G

R

T

V

I

C

H
|
H

P

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

A

I

F

A

R

Q

E

S

A

active site: L39 (= L43), C43 (= C47), C48 (= C52), S51 (= S55), V186 (≠ I187), E190 (= E191), H448 (= H445), H450 (= H447), E455 (= E452)
binding flavin-adenine dinucleotide: I10 (≠ L14), G11 (= G15), G13 (= G17), P14 (= P18), G15 (= G19), E34 (= E38), K35 (≠ R39), N36 (≠ G40), G41 (= G45), T42 (≠ V46), C43 (= C47), G47 (= G51), C48 (= C52), K52 (= K56), Y116 (≠ F118), G117 (= G119), T146 (≠ A147), G147 (= G148), S166 (= S167), I187 (= I188), F281 (≠ N278), G317 (= G314), D318 (= D315), M324 (= M321), L325 (= L322), A326 (= A323), H327 (= H324)
binding nicotinamide-adenine-dinucleotide: G183 (= G184), G185 (= G186), V205 (= V206), E206 (= E207), F207 (≠ A208), L208 (≠ M209), K240 (= K240), V241 (= V241), I276 (≠ V273), G277 (= G274), R278 (= R275), R297 (= R294), M324 (= M321)

P09622 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; EC 1.8.1.4 from Homo sapiens (Human) (see 14 papers)
40% identity, 95% coverage: 7:457/476 of query aligns to 40:497/509 of P09622

query
sites
P09622

I

I

E

D

A

A

D

D

I

V

V

T

V

V

L

I

G

G

A

S

G

G

P

P

G

G

Y

Y

T

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

K

F

K

K

V

T

V

V

V

C

V

I

E
|
E

R
x
K

G
x
N

P
x
E

A
x
T

L
|
L

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G

G

C

C

I

I

P

P

S

S

K
|
K

A

A

L

L

H

N

A

N

A

S

K

H

V

Y

L

Y

E

H

D

M

A

A

-

H

-

G

D
x
K

E

D

M

F

T

A

E

S

A

R

G

G

L

I

G

E

F

M

T

S

R

E

P

V

Q

R

L

L

D

N

L

L

D

D

K

K

L

M

R

M

R

E

W

Q

K

K

S

S

G

T

I

A

V

V

S

K

K

A

L

L

T

T

G

G

L

I

A

A

A

H

L

L

A

F

R

K

Q

Q

R

N

K

K

V

V

I

V

V

H

V

V

T

N

G

G

F

Y

G
|
G

R

K

F

I

V

T

S

G

P

K

N

N

E

Q

I

V

E

T

V

A

T

T

H

K

-

A

E

D

G

G

A

G

T

T

K

Q

R

V

V

I

A

D

F

T

T

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S
|
S

E

E

P

V

A

T

R

P

L

F

P

P

I

G

A

I

P

T

K

I

H

D

D

E

S

D

R

T

I

I

L

V

D

S

S

S

T

T

G

G

A

A

L

L

D

S

L

L

Q

K

D

K

V

V

P

P

P

E

R

K

L

M

L

V

V

V

V

I

G
|
G

G
x
A

G
|
G

I
x
V

I
|
I

G
|
G

L
x
V

E
|
E

M

L

A

G

T

S

V

V

Y

W

H

Q

A

R

L

L

G

G

S

A

R

D

V

V

T

T

V

A

V

V

E
|
E

A

F

M

L

D

G

Q

H

L

V

I

G

S

G

-

V

G

G

A

I

D

D

R

M

D

E

I

I

V

S

A

K

P

N

L

F

M

Q

K

R

R

I

I

L

S

Q

T

K

R

Q

Y

G

D

F

A

K

V

F

H

K

L

L

G

N

T

T

K

K

V
|
V

R

T

N

G

I

A

A

T

S

K

A

K

A

S

-

D

A

G

E

K

L

I

T

D

V

V

T

S

F

I

E

E

G

A

A

A

N

S

A

G

P

G

E

K

E

A

Q

E

-

V

-

I

S

T

F

C

D

D

K

V

I

L

L

L

V

V

A

C

V

I

G
|
G

R

R

V

R

P

P

N

F

S

T

G

K

T

N

L

L

G

G

L

L

A

E

A

L

G

G

V

I

A

E

V

L

D

D

Q

P

R

R

G

G

F

R

I

I

R

P

V

V

D

N

K

T

Q

R

M

F

R

Q

T

T

N

K

V

I

P

P

H

N

I

I

F

Y

A

A

I

I

G

G

D
|
D

V

V

G

A

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

D

E

E

G

G

K

I

V

I

A

C

A

V

E
|
E

V

G

A

M

A

A

G

G

L

G

K

A

A

V

A
x
H

F

I

D

D

A

Y

R

N

V

C

I

V

P

P

S

S

V

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

A

E

E

Q

A

L

N

K

A

E

N

E

G

G

V

I

A

E

Y

Y

G

K

K

V

G

G

S

K

F

F

P

P

W

F

E

A

A

A

S

N

G

S

R

R

A

A

L

K

S

T

Q

N

G

A

R

D

S

T

E

D

G

G

L

M

T

V

K

K

V

I

L

L

F

G

D

Q

P

K

E

S

T

T

H
x
D

R

R

T

V

L

L

G

G

C

A

G

H

I

I

V

L

G

G

A

P

H

G

A

A

G

G

E

E

L

M

I

V

G

N

E
|
E

M

A

V

A

L

L

A

A

I

L

E

E

M
x
Y

G

G

A

A

D

S

A

C

A

E

D
|
D

I

I

A

A

G
x
R

T

V

I

C

H
|
H

P

A

H

H

P
|
P

T

T

L

L

S
|
S

E
|
E

T

A

I

F

A
x
R

Q

E

S

A

71:80 (vs. 38:47, 50% identical) binding FAD
K72 (≠ R39) to E: in DLDD; reduced dihydrolipoyl dehydrogenase activity; no effect on interaction with PDHX; dbSNP:rs121964987
K89 (= K56) binding FAD; mutation to E: Abolishes dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.
K104 (≠ D69) to T: in dbSNP:rs1130477
G154 (= G119) binding FAD
TGS 183:185 (≠ AGS 147:149) binding FAD
220:227 (vs. 184:191, 63% identical) binding NAD(+)
E243 (= E207) binding NAD(+)
V278 (= V241) binding NAD(+)
G314 (= G274) binding NAD(+)
D355 (= D315) binding FAD
MLAH 361:364 (= MLAH 321:324) binding FAD
E375 (= E335) to K: in DLDD; loss of enzyme activity; abolished interaction with PDHX; dbSNP:rs121964992
H383 (≠ A343) mutation to A: Reduces dihydrolipoyl dehydrogenase activity.; mutation to L: Reduces dihydrolipoyl dehydrogenase activity.
D448 (≠ H408) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to N: Does not affect dihydrolipoyl dehydrogenase activity.
E466 (= E426) mutation to A: Decreases dehydrogenase activity. Loss of proteolytic activity.
Y473 (≠ M433) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to F: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to H: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.
D479 (= D439) to V: in DLDD; reduced dehydrogenase activity; increased proteolytic activity; dbSNP:rs397514649
R482 (≠ G442) to G: in DLDD; reduced enzyme activity; dbSNP:rs397514650; mutation to A: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to M: Does not affect interaction with PDHX.
H485 (= H445) mutation to A: Loss of dehydrogenase activity. Increases proteolytic activity.
P488 (= P448) to L: in DLDD; no effect on interaction with PDHX; dbSNP:rs121964988
S491 (= S451) mutation to A: Loss of proteolytic activity. Does not affect dehydrogenase activity.
E492 (= E452) mutation to Q: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.
R495 (≠ A455) to G: in DLDD; loss of enzyme activity; reduced interaction with PDHX; dbSNP:rs121964989

Sites not aligning to the query:

505 K→M: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.

6aonA 1.72 angstrom resolution crystal structure of 2-oxoglutarate dehydrogenase complex subunit dihydrolipoamide dehydrogenase from bordetella pertussis in complex with fad
41% identity, 94% coverage: 10:458/476 of query aligns to 4:463/473 of 6aonA

query
sites
6aonA

D

D

I

V

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

T

I

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

K

M

K

S

V

V

V

A

V

C

V

I

E
x
D

R
x
A

-
x
W

-

Q

-

N

-

G

-

Q

-

G

G

G

P

P

A

A

L
x
P

G

G

G
|
G

V
x
T

C
|
C

L

T

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

Q

A

S

K

E

V

H

L

Y

E

E

D

Q

A

A

D

N

E

H

-

H

M

F

T

A

E

E

A

H

G

G

L

I

G

E

F

V

T

K

R

G

P

V

Q

S

L

L

D

K

L

L

D

D

K

T

L

L

R

I

R

G

W

R

K

K

S

N

G

T

I

V

V

V

S

K

K

Q

L

N

T

N

G

D

G

G

L

I

A

L

A

Y

L

L

A

F

R

K

Q

K

R

N

K

K

V

V

I

T

V

Y

V

F

T

H

G

G

F
x
K

G
|
G

R

A

F

F

V

A

S

G

P

Q

N

V

E

D

-

G

-

W

-

S

I

I

E

K

V

V

T

T

H

G

E

T

G

T

A

D

T

A

K

D

R

L

V

V

A

A

F

-

T

K

Q

H

A

V

I

I

I

V

A

A

A
x
T

G
|
G

S

S

E

S

P

A

A

R

R

E

L

L

P

P

I

G

A

L

P

P

K

F

H

D

D

E

S

K

R

N

I

I

L

L

D

S

S
x
N

T

D

G

G

A

A

L

L

D

N

L

I

Q

G

D

A

V

V

P

P

P

K

R

K

L

L

L

G

V

V

V

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

M

A

G

T

S

V

V

Y

W

H

R

A

R

L

L

G

G

S

A

R

E

V

V

T

T

V

I

V

L

E

E

A

A

M

M

D

P

Q

E

L

F

I

L

S
x
A

G

A

A
|
A

D

D

R
x
Q

D

Q

I

V

V

A

A

K

P

E

L

A

M

L

K

K

R

S

I

F

S

A

T

K

R

Q

Y

G

D

L

A

D

V

I

H

Q

L

T

G

G

T

V

K

K

V

I

R

G

N

E

I

I

A

K

S

A

A

A

A

K

E

S

L

I

T

T

V

V

T

P

F

Y

E

V

G

D

A

A

N

K

A

G

P

A

E

E

E

Q

Q

K

-

L

S

V

F

V

D

D

K

K

I

L

L

I

V

V

A

S

V

I

G

G

R
|
R

V

V

P

P

N
x
Y

S

T

G

G

T

G

L

L

G

N

L

A

A

E

A

A

A

V

G

G

V

L

A

K

V

L

D

D

Q

E

R

R

G

G

F

F

I

V

R

A

V

V

D

D

K

E

Q

D

M

C

R

K

T

T

N

N

V

L

P

P

H

N

I

V

F

W

A

A

I

V

G
|
G

D
|
D

V

V

G

R

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

V

E

H

E

E

E

G

G

K

V

V

A

A

V

A

A

E

E

V

R

A

I

A

A

G

G

L

Q

K

H

A

G

A

H

F

V

D

N

A

F

R

A

V

T

I

V

P

P

S

W

V

V

A

I

Y

Y

T

T

D

S

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

A

Q

E

Q

A

L

N

K

A

A

N

E

G

G

V

R

A

E

Y

Y

G

K

K

A

G

G

S

S

F

F

P

P

W

F

E

M

A

A

S

N

G

G

R

R

A

A

L

R

S

A

Q

L

G

G

R

D

S

T

E

T

G

G

L

F

T

A

K

K

V

V

L

I

F

A

D

D

P

A

E

K

T

T

H

D

R

E

T

V

L

L

G

G

C

V

G

H

I

I

V

I

G

G

A

P

H

M

A

A

G

S

E

E

L

L

I

I

G

S

E

E

M

A

V

V

L

T

A

I

I

M

E

E

M

F

G

R

A

G

D

A

A

A

A

E

D

D

I

I

A

A

G

R

T

I

I

C

H
|
H

P

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

T

A

I

V

A

K

Q

E

S

A

A

A

active site: P43 (≠ L43), C47 (= C47), C52 (= C52), S55 (= S55), V191 (≠ I187), E195 (= E191), H450 (= H445), H452 (= H447), E457 (= E452)
binding calcium ion: A218 (≠ S214), A220 (= A216), Q222 (≠ R218)
binding flavin-adenine dinucleotide: I8 (≠ L14), G11 (= G17), P12 (= P18), G13 (= G19), D32 (≠ E38), A33 (≠ R39), W34 (vs. gap), G45 (= G45), T46 (≠ V46), C47 (= C47), G51 (= G51), C52 (= C52), K56 (= K56), K119 (≠ F118), G120 (= G119), T151 (≠ A147), G152 (= G148), N171 (≠ S167), I192 (= I188), R280 (= R275), Y283 (≠ N278), G319 (= G314), D320 (= D315), M326 (= M321), L327 (= L322), A328 (= A323), H329 (= H324)

Query Sequence

>WP_083509555.1 NCBI__GCF_001541235.1:WP_083509555.1
MVRAVDIEADIVVLGAGPGGYTAAFRAADLGKKVVVVERGPALGGVCLNVGCIPSKALLH
AAKVLEDADEMTEAGLGFTRPQLDLDKLRRWKSGIVSKLTGGLAALARQRKVIVVTGFGR
FVSPNEIEVTHEGATKRVAFTQAIIAAGSEPARLPIAPKHDSRILDSTGALDLQDVPPRL
LVVGGGIIGLEMATVYHALGSRVTVVEAMDQLISGADRDIVAPLMKRISTRYDAVHLGTK
VRNIASAAAELTVTFEGANAPEEQSFDKILVAVGRVPNSGTLGLAAAGVAVDQRGFIRVD
KQMRTNVPHIFAIGDVVGQPMLAHKAVHEGKVAAEVAAGLKAAFDARVIPSVAYTDPEVA
WVGLTEAEANANGVAYGKGSFPWEASGRALSQGRSEGLTKVLFDPETHRTLGCGIVGAHA
GELIGEMVLAIEMGADAADIAGTIHPHPTLSETIAQSAEMFEGTITDLIARQARQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory