SitesBLAST
Comparing WP_083758313.1 NCBI__GCF_000019185.1:WP_083758313.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
41% identity, 88% coverage: 15:281/303 of query aligns to 15:280/296 of 3w6zA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G20), L21 (= L21), G22 (= G22), I23 (≠ V23), M24 (= M24), N43 (= N43), R44 (= R44), T45 (= T45), K48 (= K48), V77 (= V78), S78 (≠ G79), D82 (= D83), Q85 (= Q86), V133 (= V134), F244 (= F245), K245 (≠ A246), H248 (≠ W249), K251 (= K252)
3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
41% identity, 88% coverage: 15:281/303 of query aligns to 15:277/293 of 3ws7A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G20), L21 (= L21), G22 (= G22), I23 (≠ V23), M24 (= M24), N43 (= N43), R44 (= R44), T45 (= T45), K48 (= K48), M76 (≠ C77), V77 (= V78), S78 (≠ G79), D82 (= D83), Q85 (= Q86), V133 (= V134), F241 (= F245), K242 (≠ A246), H245 (≠ W249), K248 (= K252)
- binding sulfate ion: T134 (≠ S135), G135 (= G136), K183 (= K184)
3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
32% identity, 95% coverage: 13:299/303 of query aligns to 1:286/287 of 3pduA
- binding glycerol: R242 (≠ G255), E246 (= E259), E246 (= E259), R250 (= R263)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G20), G10 (= G22), I11 (≠ V23), M12 (= M24), N31 (= N43), R32 (= R44), N33 (≠ T45), M64 (≠ C77), L65 (≠ V78), A66 (≠ G79), A70 (≠ D83), T96 (= T109), V121 (= V134), G123 (= G136), T124 (≠ G137), K171 (= K184), S231 (≠ G244), F232 (= F245), P233 (≠ A246), H236 (≠ W249), K239 (= K252)
5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
29% identity, 96% coverage: 11:300/303 of query aligns to 1:291/294 of 5je8B
2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
34% identity, 95% coverage: 13:300/303 of query aligns to 1:282/289 of 2cvzC
- active site: S117 (= S135), K165 (= K184), N168 (= N187), N169 (≠ Q188)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G20), L9 (= L21), G10 (= G22), A11 (≠ V23), M12 (= M24), N30 (= N43), R31 (= R44), T32 (= T45), C62 (= C77), L63 (≠ V78), P64 (≠ G79), E68 (≠ D83), E71 (≠ Q86), S91 (≠ T109), V116 (= V134), F227 (= F245), K234 (= K252)
1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
34% identity, 94% coverage: 15:300/303 of query aligns to 2:281/288 of 1wp4A
- active site: S116 (= S135), K164 (= K184), N167 (= N187), N168 (≠ Q188)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G20), L8 (= L21), G9 (= G22), A10 (≠ V23), M11 (= M24), N29 (= N43), R30 (= R44), T31 (= T45), K34 (= K48), C61 (= C77), L62 (≠ V78), P63 (≠ G79), E67 (≠ D83), S90 (≠ T109), V115 (= V134), T225 (≠ G244), F226 (= F245), K233 (= K252)
- binding sulfate ion: S116 (= S135), G117 (= G136), G118 (= G137), K164 (= K184)
2uyyA Structure of the cytokine-like nuclear factor n-pac
28% identity, 96% coverage: 5:294/303 of query aligns to 2:286/292 of 2uyyA
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: G15 (= G22), L16 (≠ V23), M17 (= M24), N36 (= N43), R37 (= R44), T38 (= T45), V70 (= V78), S71 (≠ G79), A75 (≠ D83), T101 (= T109), F237 (= F245), Y238 (≠ A246), Y241 (≠ W249), K244 (= K252)
P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
30% identity, 94% coverage: 16:300/303 of query aligns to 41:331/335 of P29266
- D68 (≠ N43) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
- K208 (= K184) mutation K->A,H,N,R: Complete loss of activity.
- N212 (≠ Q188) mutation to Q: Decrease in activity.
Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
28% identity, 96% coverage: 5:294/303 of query aligns to 263:547/553 of Q49A26
- 271:285 (vs. 17:31, 40% identical) binding NAD(+)
- T362 (= T109) binding NAD(+)
- M437 (≠ K184) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.
- P496 (≠ F243) to L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
- K505 (= K252) binding NAD(+)
Sites not aligning to the query:
- 214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
- 214:217 Interaction with histone H3
- 216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
- 216:225 Interaction with KDM1B
- 217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
- 219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
- 220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
- 223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.
Q922P9 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Putative oxidoreductase GLYR1 from Mus musculus (Mouse) (see paper)
28% identity, 96% coverage: 5:294/303 of query aligns to 262:540/546 of Q922P9
- P489 (≠ F243) mutation to L: Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.
2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
30% identity, 94% coverage: 16:300/303 of query aligns to 3:293/296 of 2i9pB
- binding nicotinamide-adenine-dinucleotide: G9 (= G22), N10 (≠ V23), M11 (= M24), Y29 (= Y42), D30 (≠ N43), V31 (≠ W52), M63 (≠ C77), L64 (≠ V78), P65 (≠ G79), T95 (= T109), V120 (= V134), G122 (= G136), F238 (= F245), K245 (= K252)
P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
30% identity, 94% coverage: 16:300/303 of query aligns to 42:332/336 of P31937
- LP 103:104 (≠ VG 78:79) binding NAD(+)
- N108 (≠ D83) binding NAD(+)
- T134 (= T109) binding NAD(+)
- K284 (= K252) binding NAD(+)
Sites not aligning to the query:
- 1:36 modified: transit peptide, Mitochondrion
- 40:68 binding NAD(+)
3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
29% identity, 94% coverage: 15:300/303 of query aligns to 3:287/287 of 3pefA
- binding glycerol: D67 (≠ N80), G123 (= G136), K171 (= K184), N175 (≠ Q188), M178 (≠ I191), L203 (≠ V216), G207 (≠ S220), N213 (≠ S226), A217 (≠ E230), F232 (= F245), H236 (≠ W249), K239 (= K252), R242 (≠ G255), R269 (≠ Q282)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G22), I11 (≠ V23), M12 (= M24), N31 (= N43), R32 (= R44), S33 (≠ T45), K36 (= K48), M64 (≠ C77), L65 (≠ V78), A66 (≠ G79), A70 (≠ D83), E73 (≠ Q86), T96 (= T109), V121 (= V134), G123 (= G136), S124 (≠ G137), A231 (≠ G244), F232 (= F245), H236 (≠ W249), K239 (= K252)
P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
30% identity, 96% coverage: 13:303/303 of query aligns to 1:291/298 of P0A9V8
- QM 11:12 (≠ VM 23:24) binding NAD(+)
- D31 (≠ N43) binding NAD(+)
- L65 (≠ V78) binding NAD(+)
- T96 (= T109) binding NAD(+)
- G122 (≠ S135) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
- R123 (≠ G136) binding 2,3-dihydroxypropane-1-sulfonate; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
- T124 (≠ G137) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
- NNYMS 174:178 (≠ NQICI 187:191) binding 2,3-dihydroxypropane-1-sulfonate
- K240 (= K252) binding NAD(+)
6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
29% identity, 96% coverage: 14:303/303 of query aligns to 1:290/295 of 6smzC