SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_083866922.1 NCBI__GCF_000058485.1:WP_083866922.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
46% identity, 92% coverage: 33:601/621 of query aligns to 93:654/664 of P09114

query
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P09114

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P191 (≠ A131) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W510) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
46% identity, 92% coverage: 33:601/621 of query aligns to 96:657/667 of P09342

query
sites
P09342

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C161 (= C98) modified: Disulfide link with 307
P194 (≠ A131) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V246) modified: Disulfide link with 161

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
45% identity, 92% coverage: 33:601/621 of query aligns to 13:574/582 of 3ea4A

query
sites
3ea4A

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x
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-

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P

D

N

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A

A

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Q

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A

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active site: Y32 (≠ I52), G34 (= G54), G35 (= G55), A36 (= A56), S37 (≠ I57), E58 (= E78), T81 (= T101), F120 (= F140), Q121 (= Q141), E122 (= E142), K170 (= K190), M265 (= M289), V292 (= V316), V399 (= V421), G425 (= G447), M427 (= M449), D452 (= D474), N479 (= N501), H481 (≠ S503), L482 (= L504), M484 (= M506), V485 (= V507), W488 (= W510), H557 (≠ P584)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D314), R291 (= R315), W488 (= W510), S567 (≠ A594)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R180), G221 (= G243), G222 (= G244), G223 (= G245), T245 (= T269), L246 (= L270), M247 (= M271), L263 (≠ P287), G264 (= G288), M265 (= M289), H266 (= H290), G285 (= G309), R287 (= R311), D289 (= D313), R291 (= R315), D309 (= D333), I310 (= I334), G327 (= G351), D328 (= D352), V329 (≠ C353), M404 (= M426), G422 (= G444)
binding magnesium ion: D452 (= D474), N479 (= N501), H481 (≠ S503)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V421), G400 (= G422), Q401 (= Q423), H402 (= H424), M427 (= M449), G451 (= G473), D452 (= D474), G453 (= G475), S454 (≠ C476), N479 (= N501), H481 (≠ S503), L482 (= L504), G483 (= G505), M484 (= M506), V485 (= V507)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
45% identity, 92% coverage: 33:601/621 of query aligns to 13:574/582 of 3e9yA

query
sites
3e9yA

G

G

A

A

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T

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-

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I

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H

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active site: Y32 (≠ I52), G34 (= G54), G35 (= G55), A36 (= A56), S37 (≠ I57), E58 (= E78), T81 (= T101), F120 (= F140), Q121 (= Q141), E122 (= E142), K170 (= K190), M265 (= M289), V292 (= V316), V399 (= V421), G425 (= G447), M427 (= M449), D452 (= D474), N479 (= N501), H481 (≠ S503), L482 (= L504), M484 (= M506), V485 (= V507), W488 (= W510), H557 (≠ P584)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D314), R291 (= R315), W488 (= W510), S567 (≠ A594)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R180), G221 (= G243), G222 (= G244), G223 (= G245), T245 (= T269), L246 (= L270), M247 (= M271), L263 (≠ P287), G285 (= G309), R287 (= R311), D289 (= D313), R291 (= R315), D309 (= D333), I310 (= I334), G327 (= G351), D328 (= D352), V329 (≠ C353), M404 (= M426), G422 (= G444)
binding magnesium ion: D452 (= D474), N479 (= N501), H481 (≠ S503)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V421), G400 (= G422), Q401 (= Q423), H402 (= H424), M427 (= M449), G451 (= G473), G453 (= G475), S454 (≠ C476), N479 (= N501), H481 (≠ S503), L482 (= L504), G483 (= G505), M484 (= M506), V485 (= V507)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
45% identity, 92% coverage: 33:601/621 of query aligns to 14:575/582 of 7tzzA

query
sites
7tzzA

G

G

A

A

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L

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E

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A

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L

E

E

A

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G

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H

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A

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A

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E

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C

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A

A

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S

G

G

P

P

G

G

A

A

T

T

N

N

L

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P

G

I

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A

A

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D

A

A

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L

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A

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M289), R292 (= R315), W489 (= W510), S568 (≠ A594)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V421), G401 (= G422), Q402 (= Q423), H403 (= H424), G426 (= G447), M428 (= M449), G452 (= G473), D453 (= D474), G454 (= G475), S455 (≠ C476), L483 (= L504), G484 (= G505), M485 (= M506), V486 (= V507)
binding flavin-adenine dinucleotide: R161 (= R180), G222 (= G243), G223 (= G244), G224 (= G245), T246 (= T269), L247 (= L270), M248 (= M271), M263 (= M286), L264 (≠ P287), M266 (= M289), H267 (= H290), G286 (= G309), R288 (= R311), V293 (= V316), D310 (= D333), I311 (= I334), D329 (= D352), V330 (≠ C353), M405 (= M426), G423 (= G444)
binding magnesium ion: A37 (= A56), T82 (= T101), S83 (= S102), Q122 (= Q141), Y381 (≠ H402), D453 (= D474), M458 (= M479), Q461 (= Q482), N480 (= N501), H482 (≠ S503), K533 (≠ A558)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
45% identity, 92% coverage: 33:601/621 of query aligns to 99:660/670 of P17597

query
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P17597

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A122 (= A56) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L58) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E78) binding thiamine diphosphate
S186 (= S120) binding FAD
P197 (≠ A131) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A133) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q141) binding thiamine diphosphate
K220 (= K154) binding (R)-imazaquin
R246 (= R180) binding (R)-imazaquin; binding FAD
K256 (= K190) binding chlorimuron-ethyl
G308 (= G244) binding FAD
TL 331:332 (= TL 269:270) binding FAD
C340 (≠ D278) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ PGMH 287:290) binding FAD
GVRFD 371:375 (≠ GARFD 309:313) binding FAD
DR 376:377 (= DR 314:315) binding chlorimuron-ethyl
DI 395:396 (= DI 333:334) binding FAD
DV 414:415 (≠ DC 352:353) binding FAD
QH 487:488 (= QH 423:424) binding thiamine diphosphate
GG 508:509 (= GG 444:445) binding FAD
GAM 511:513 (≠ GTM 447:449) binding thiamine diphosphate
D538 (= D474) binding Mg(2+)
DGS 538:540 (≠ DGC 474:476) binding thiamine diphosphate
N565 (= N501) binding Mg(2+)
NQHLGM 565:570 (≠ NGSLGM 501:506) binding thiamine diphosphate
H567 (≠ S503) binding Mg(2+)
W574 (= W510) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A594) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
45% identity, 92% coverage: 33:601/621 of query aligns to 14:575/582 of 8et4A

query
sites
8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V421), G401 (= G422), Q402 (= Q423), H403 (= H424), G426 (= G447), M428 (= M449), G452 (= G473), D453 (= D474), G454 (= G475), S455 (≠ C476), M458 (= M479), N480 (= N501), H482 (≠ S503), L483 (= L504), G484 (= G505), M485 (= M506), V486 (= V507)
binding flavin-adenine dinucleotide: R161 (= R180), G222 (= G243), G223 (= G244), G224 (= G245), T246 (= T269), L247 (= L270), M248 (= M271), L264 (≠ P287), M266 (= M289), H267 (= H290), G286 (= G309), V287 (≠ A310), R288 (= R311), D290 (= D313), R292 (= R315), V293 (= V316), D310 (= D333), I311 (= I334), D329 (= D352), V330 (≠ C353), M405 (= M426), G423 (= G444)
binding magnesium ion: F370 (≠ Y390), D453 (= D474), M458 (= M479), Q461 (= Q482), N480 (= N501), H482 (≠ S503), K533 (≠ A558)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M289), R292 (= R315), M485 (= M506), W489 (= W510), S568 (≠ A594)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
45% identity, 92% coverage: 33:601/621 of query aligns to 14:575/582 of 5wj1A

query
sites
5wj1A

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